Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0012478

Physical Location & Seq

Genomic Coordinates Reverse (-)

10636339 .. 10643209

Gene Structure 6871 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1116300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3675 bp

ATGGAGAAGGGTCTTCTGATCGACAATAATCATAAGAGGAATGGTCCTATTATTGCTTCCAAGATGGTGGGATCATTTGTTAAAATTGATTCAATATCTATAGATCTTCCTGATGCTAATGACAAGGGTAGCAGCCATGTGGGAAACTGCGAGCATTTCAGTATACGTGGTTATGTAGCTGAAGTTCGCAAAAAGAATTGGAAGCTTTGTTGTCCATTTCCTCTGGATGGTGAGTTGGACAAGTTTGATCAGGAGCCAACATCCTTGCTTCCTCCTTTGGATCCTCCAAAATTCAGAAGGTGGTCTTGCCAGAATTGCGTGCTGGAAACTGGTTCTGAGAGTATTGCAAGAGATCATGGTACAGACATTCGTATTGGATCTGATTCCAATAACAAGTGTGCTCAGGTCCCACACCACAGTGATGCTCCACTGCTTCTGTTAGATTCTGAACAAGGTTCAATTTCAAAAACTGTTGATGGAAAAAGAGTTGAAGTTTATGTTTCTGTTGATGGAATTGGCAATAAGCAACATCCTTCGTCATCTCCCTTTGATAAGAAAGAAGGGAGGCATGAAGTTGCACCCACTGCCATCACAGTGAATAAGAATGTCTTAGATGATTATGCGAGTCATGAAATGCCGAGACTCAATTCTGTTGAGGCAGAAGTTGACCCAAAAAGGACGCAGGAGAGACATAAAGAAGCAGTAAATTTGGAGGGCAATGGATCTGTTGAAATCTGGAAATCAGGATGTGAAAGTCGTGATGATACCAACATTAAGCTCCATTTCTCAAACAACAAATTATTCTGGAATAAGAACCTCACCCAGACTAGTGAAGCCGAGCGCCGACCTTCTGCAGAAGAAAATCACAAGGAGTCAATGAATGCCTCTGGGACATGTAAGATAGTCGGAACTGTTGACAGGGCAGGTGATGCTACCAAAGGCCAGACCAACATTAATCATCCTCTGCATGAGTGTGATTCTGCATCTTCTGAAAATCTTGATAAGTCAAGTAGTTTGAATCGTAAGAAAGGTAGGAAGGTGCGTGTGCGTCTGCTGAGTGAGTTGGGTGATGCAAAGCCTGATTTCTTCAGGACGGAAGATTCTCCATCCAACAGAATTAGTAATACATCTGGGGCAGGAACTGTTTCCCAAGGCCAGGTGTCTGTCCAAGAAAGTGCTATAGTGGATCTTGGTGAGAATAACAAAAGGAAATTGCCTGAGGAAGAGGAACAGAGAAGCCAAGGGACGAGCCTTCCAAAAAGTTTGAATAGAAAAGTTAAGACTTCCAAGCGAGATGAAGAAACCACCAGACGCGTTGAGGATTCTGAATTAGAAGAAGATGCAGTTGCCAGAATATCTTTGCAGGCAGATATGAGGAGCAACTGGAATAGATCTGAAATTGAACGAATCCCGGCTGTTGGTAAGAAGAAAATTATGAAATCCAATGATATTGGTGTATGTTCATCCTTAGTCCCACCCAAAGATATTGTGCCAATAGAAGCGCTAGACAAAGTTGGAAGTTCAAGTAAAGGTAATGCTACTACAATAGCTTCTTCTATTCCTGCGTATGATGCATCCACAGTTAGAGAGATAGATCAAATTGCTTTGCCTGCCCCAAAAGCAGGTAGAAAGTCTGGTTCCTGTAAGCGGAAAGACAAGATGCCAGAAGTTGATACTGGACTGGCTTCTCTATTTCCTTGGCTCACGAAAGGCCCAAATACAAGGAAAGATGCAGAATTCATGCAAACTGGGCATCCATTTCATCGGGCAGAGGACTCACCCGCTGAAAAAGGGTTGAATCTTTCTCTTAACAGCTATTTGTCTGCTCAAAGAGATGACAGGAAATTTATTCCTCAATCCGAAGATGGATTTCCTTCCTGGTCAACTCGGAAAGATGGCACCGGCAAACTAGATGAGTTTATTAGGAAAAATGCGGAAGCCAACGCAAATCTGAACAGACTTCCTAAACCTGTTTCAGATGCATTTTCTGGGAAAATAGGGCATGATGATCGCAGTAGTAAATTTTTCACCTACAGCATGCCTATCCCGAATGAGAAGCAAAACTATCGCTCCCAGGTTGAAGGGAGTTTGTTGCAGCAAATGGATATTTCTCGTGCAAGAAACAAGGAGAAAATCATTGAGGTTCCGAAAAATTCAGCTGTGCCTGTTAGTAGGAAAGATAGCAACCATAGAGCTGAAAAGACGTCTCATAAAGGAGCTGCAGATGACATACCGATGGAAATTGTTGAATTCATGGCAAAAAATCATTATGAGAGAAATCAGAAAGTTTTGGAAGCAGATCATAGTTATGGCTTTGGAAACTTAAGAATATTAGAGGAAACAGGTCAGAAACGAAAACCTCAGGCAAGAAAGGCAAAAGATGGTGCAACAGCAAGAAAAAATGTGGGACCTTCCAAACAGAAGTTAGTTGACCTTTCTACTTATATCCCCGAAAAAGACACATTTCGTCTTGATCAAATGCATTGCCCTACAGGGTTTAATGCATTCAGTCAGCCTAAAAAGAAGATGAGTACTCGAGAGCAATTTCCTACTGCTGGTTACAGCAGCTGTAGTTGTGCTCAAAATTGCAAATGGAATGGAGATATGATGAGCCATGGGTTGTCAAATTCTAGCTTGCGTCCGTCTGTTCCACAATCAAAGGAAGAAGCAGCTCGCTTATGGTCACCTGCAATGCCAGCTCAAACTCCCTTAATGCATTACAATCCTCAAAAGTGTGCAGCTCAGCCTTCTAATGTCGATATGCTTTCACACCCTCCAGGCTCGCTGCATAAGGGAAATTTCCATGGTGATTATGGCCTGAAATTATTTAATTTAAATGCTACCAACCCGGAAAAGCATATTGAAGGTGTTGGTTCTGAACCTTTCAGCAGAACAAATGCAGAGTACTCATTCACTGGCAAACGTAACGGGACTGAGCCTCCTAAGAGTTCAGCGGGTTCTTTTGATCTATATTCCAATGAAACCATACCAGCAATGCATTTGCTCAGCCTCATGGATGCAGGTATGCGGTCAAGTCCGCCCTTCAGCATTGCTGGAAACCCGAAATTTCCCAAGAGACCCATTGATTTTTACTCGAAGGAGTATTCTGCGGTGGATGCTGGTGGTTATAAGGTTACCGATACTATGAAGCATCCATCATCCAGTTGTTGCGGCAAAAATCACATTTCTGAGAAGTCTCTTGACCTTTTTCCTATGAATCCTGGTGGTACATCTACCTCTTCCTATCCTAAAGGATTCAAAAGGGCTGCCGTAGAGAATAGAGGCTCCAGATCCCAGAATTCTGCATTTAAAGGTGGTGTTTTGGGCAAAAATCATGGAACTATCCCTGTTATGCACAAAGGACTCGCTACACTGGCTGATTCTACGATGTTTCCCCAGCACCACCACAACACTGAATATCCTATGCTGCAGAAATTAGATACTCATAATGCTAATGGGGCCATGAGGCCTCCAAGGAGCAGTCCCAATTCTTTAATCTGCACTATAAACAAAAACCCAGCTGAGTTTAGCTTGCCAGAAGATGGCAATGAGTACATGATTAGCGGGAAAGACTTAAAAGTTGGGAAGCAGAGGCATGGCTTTGTACAAGTAGATCAGAACAAGCAGCTGAAGAAGAGGAAACAGACTACAGGAAAAGGACGCGCACAACAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008213 GO:0009628 GO:0009651 GO:0009791 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010022 GO:0010073 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032501 GO:0032502 GO:0034968 GO:0036211 GO:0042538 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048367 GO:0048507 GO:0048519 GO:0048523 GO:0048580 GO:0048581 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0065007 GO:0070734 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

1225

Amino Acids

135.28

Weight (kDa)

9.07

Isoelectric Point (pI)

51.16

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012478)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G11530 AT5G11530 AT5G11530
fragaria_vesca	FvH4_2g35880 FvH4_2g35880
malus_domestica	MD08G1116300.v1.1 MD15G1096000.v1.1
prunus_persica	Prupe.1G450900_v2.0.a1
pyrus_communis	pycom08g09700
rosa_chinensis	RchiOBHm_Chr6g0305941
rosa_laevigata	RLG00000010844
rosa_multiflora	Rmu_sc0001809.1_g000052
rosa_roxburghii	Rroxscaffold_7G00162200
rosa_rugosa	Rorug06G0345500
rosa_samantha	Rh6AG457200 Rh6BG443900 Rh6CG471200 Rh6DG457700
rosa_wichuraiana	Rw6G039890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3129
AarI	CACCTGC	2 cut(s)	914, 2695
AatII	GACGTC	1 cut(s)	2207
Acc36I	ACCTGC	4 cut(s)	914, 1613, 2695, 3011
AccB1I	GGYRCC	1 cut(s)	1898
AccB7I	CCANNNNNTGG	1 cut(s)	3542
AccI	GTMKAC	1 cut(s)	163
AccII	CGCG	2 cut(s)	1314, 3663
AciI	CCGC	9 cut(s)	1648, 1782, 1934, 2954, 3028, 3038, 3110, 3171, 3564
AclWI	GGATC	7 cut(s)	79, 275, 288, 385, 730, 1194, 3285
AcuI	CTGAAG	4 cut(s)	201, 1072, 3028, 3650
AcyI	GRCGYC	1 cut(s)	2204
AfaI	GTAC	6 cut(s)	361, 2533, 2906, 3229, 3554, 3606
AfeI	AGCGCT	1 cut(s)	1503
AfiI	CCNNNNNNNGG	7 cut(s)	227, 1156, 1418, 1622, 1647, 2555, 3542
AflII	CTTAAG	1 cut(s)	2323
AflIII	ACRYGT	2 cut(s)	893, 1312
AhlI	ACTAGT	1 cut(s)	827
AjnI	CCWGG	5 cut(s)	1155, 1877, 2073, 2776, 3220
AleI	CACNNNNGTG	1 cut(s)	417
Alw21I	GWGCWC	2 cut(s)	403, 2581
Alw26I	GTCTC	5 cut(s)	634, 682, 2211, 3070, 3201
AlwI	GGATC	7 cut(s)	79, 275, 288, 385, 730, 1194, 3285
AlwNI	CAGNNNCTG	2 cut(s)	1142, 2568
Ama87I	CYCGRG	1 cut(s)	2535
Aor51HI	AGCGCT	1 cut(s)	1503
AoxI	GGCC	6 cut(s)	940, 1153, 1711, 2815, 3459, 3467
AseI	ATTAAT	1 cut(s)	954
Asp700I	GAANNNNTTC	1 cut(s)	1144
AspLEI	GCGC	3 cut(s)	843, 1504, 3665
AspS9I	GGNCC	5 cut(s)	44, 406, 1712, 2408, 3459
AsuC2I	CCSGG	2 cut(s)	1412, 2849
AsuHPI	GGTGA	9 cut(s)	242, 811, 938, 1079, 1205, 1770, 2020, 2676, 2819
AvaI	CYCGRG	1 cut(s)	2535
AvaII	GGWCC	3 cut(s)	44, 406, 2408
AxyI	CCTNAGG	2 cut(s)	1218, 2361
BamHI	GGATCC	1 cut(s)	280
BanI	GGYRCC	1 cut(s)	1898
BauI	CACGAG	1 cut(s)	2112
BbsI	GAAGAC	1 cut(s)	5
Bbv12I	GWGCWC	2 cut(s)	403, 2581
BceAI	ACGGC	1 cut(s)	3254
BcgI	CGANNNNNNTGC	4 cut(s)	1991, 2025, 2048, 2082
BciT130I	CCWGG	5 cut(s)	1157, 1879, 2075, 2778, 3222
BclI	TGATCA	2 cut(s)	247, 2473
BcnI	CCSGG	2 cut(s)	1412, 2849
BcoDI	GTCTC	5 cut(s)	634, 682, 2211, 3070, 3201
BcuI	ACTAGT	1 cut(s)	827
BfaI	CTAG	4 cut(s)	828, 1505, 1910, 2631
BfmI	CTRYAG	9 cut(s)	99, 852, 1179, 2032, 2220, 2490, 2569, 3428, 3648
BfoI	RGCGCY	2 cut(s)	844, 1505
BfrI	CTTAAG	1 cut(s)	2323
BfuAI	ACCTGC	4 cut(s)	914, 1613, 2695, 3011
BglII	AGATCT	2 cut(s)	103, 1391
BlpI	GCTNAGC	2 cut(s)	2742, 3005
BmcAI	AGTACT	2 cut(s)	2533, 2906
Bme1390I	CCNGG	7 cut(s)	1157, 1412, 1879, 2075, 2778, 2849, 3222
Bme18I	GGWCC	3 cut(s)	44, 406, 2408
BmeT110I	CYCGRG	1 cut(s)	2535
BmgT120I	GGNCC	5 cut(s)	44, 406, 1712, 2408, 3459
BmiI	GGNNCC	9 cut(s)	255, 282, 408, 1639, 1900, 2145, 2409, 3286, 3460
BmrFI	CCNGG	7 cut(s)	1157, 1412, 1879, 2075, 2778, 2849, 3222
BmrI	ACTGGG	1 cut(s)	1758
BmuI	ACTGGG	1 cut(s)	1758
BpiI	GAAGAC	1 cut(s)	5
BpmI	CTGGAG	2 cut(s)	2760, 3271
Bpu10I	CCTNAGC	1 cut(s)	402
Bpu1102I	GCTNAGC	2 cut(s)	2742, 3005
BpuMI	CCSGG	2 cut(s)	1412, 2849
BsaAI	YACGTR	1 cut(s)	167
BsaHI	GRCGYC	1 cut(s)	2204
BsaI	GGTCTC	1 cut(s)	3070
BsaJI	CCNNGG	7 cut(s)	1150, 1240, 1697, 2073, 2614, 2803, 3473
BsaXI	ACNNNNNCTCC	4 cut(s)	2054, 2084, 2923, 2953
Bsc4I	CCNNNNNNNGG	7 cut(s)	227, 1156, 1418, 1622, 1647, 2555, 3542
Bse118I	RCCGGY	1 cut(s)	1901
Bse1I	ACTGG	8 cut(s)	334, 1388, 1681, 1686, 1753, 2920, 3162, 3378
Bse21I	CCTNAGG	2 cut(s)	1218, 2361
Bse3DI	GCAATG	6 cut(s)	724, 2482, 2697, 3000, 3048, 3553
BseBI	CCWGG	5 cut(s)	1157, 1879, 2075, 2778, 3222
BseDI	CCNNGG	7 cut(s)	1150, 1240, 1697, 2073, 2614, 2803, 3473
BseLI	CCNNNNNNNGG	7 cut(s)	227, 1156, 1418, 1622, 1647, 2555, 3542
BseMI	GCAATG	6 cut(s)	724, 2482, 2697, 3000, 3048, 3553
BseNI	ACTGG	8 cut(s)	334, 1388, 1681, 1686, 1753, 2920, 3162, 3378
BseRI	GAGGAG	1 cut(s)	1390
BseYI	CCCAGC	2 cut(s)	3396, 3517
BsgI	GTGCAG	2 cut(s)	2757, 3484
Bsh1236I	CGCG	2 cut(s)	1314, 3663
BshFI	GGCC	6 cut(s)	942, 1155, 1713, 2817, 3461, 3469
BshNI	GGYRCC	1 cut(s)	1898
BsiHKAI	GWGCWC	2 cut(s)	403, 2581
BsiHKCI	CYCGRG	1 cut(s)	2535
BsiSI	CCGG	3 cut(s)	1412, 1902, 2849
BslFI	GGGAC	7 cut(s)	392, 904, 1258, 1457, 2421, 2944, 3468
BslI	CCNNNNNNNGG	7 cut(s)	227, 1156, 1418, 1622, 1647, 2555, 3542
BsmAI	GTCTC	5 cut(s)	634, 682, 2211, 3070, 3201
BsmBI	CGTCTC	1 cut(s)	2211
BsmFI	GGGAC	7 cut(s)	392, 904, 1258, 1457, 2421, 2944, 3468
BsmI	GAATGC	2 cut(s)	886, 2504
BsnI	GGCC	6 cut(s)	942, 1155, 1713, 2817, 3461, 3469
Bso31I	GGTCTC	1 cut(s)	3070
BsoBI	CYCGRG	1 cut(s)	2535
Bsp1286I	GDGCHC	2 cut(s)	403, 2581
Bsp1407I	TGTACA	1 cut(s)	3604
Bsp1720I	GCTNAGC	2 cut(s)	2742, 3005
Bsp19I	CCATGG	2 cut(s)	2614, 2803
BspACI	CCGC	9 cut(s)	1648, 1782, 1934, 2954, 3028, 3038, 3110, 3171, 3564
BspANI	GGCC	6 cut(s)	942, 1155, 1713, 2817, 3461, 3469
BspFNI	CGCG	2 cut(s)	1314, 3663
BspHI	TCATGA	1 cut(s)	628
BspLI	GGNNCC	9 cut(s)	255, 282, 408, 1639, 1900, 2145, 2409, 3286, 3460
BspMAI	CTGCAG	3 cut(s)	856, 2224, 3432
BspMI	ACCTGC	4 cut(s)	914, 1613, 2695, 3011
BspPI	GGATC	7 cut(s)	79, 275, 288, 385, 730, 1194, 3285
BspT107I	GGYRCC	1 cut(s)	1898
BspTI	CTTAAG	1 cut(s)	2323
BspTNI	GGTCTC	1 cut(s)	3070
BsrDI	GCAATG	6 cut(s)	724, 2482, 2697, 3000, 3048, 3553
BsrFI	RCCGGY	1 cut(s)	1901
BsrGI	TGTACA	1 cut(s)	3604
BsrI	ACTGG	8 cut(s)	334, 1388, 1681, 1686, 1753, 2920, 3162, 3378
BssAI	RCCGGY	1 cut(s)	1901
BssECI	CCNNGG	7 cut(s)	1150, 1240, 1697, 2073, 2614, 2803, 3473
BssNAI	GTATAC	1 cut(s)	164
BssNI	GRCGYC	1 cut(s)	2204
BssSI	CACGAG	1 cut(s)	2112
BssT1I	CCWWGG	6 cut(s)	1150, 1240, 1697, 2614, 2803, 3473
Bst1107I	GTATAC	1 cut(s)	164
Bst2BI	CACGAG	1 cut(s)	2112
Bst2UI	CCWGG	5 cut(s)	1157, 1879, 2075, 2778, 3222
Bst4CI	ACNGT	6 cut(s)	419, 472, 595, 913, 1144, 1582
Bst6I	CTCTTC	3 cut(s)	1218, 3244, 3629
BstACI	GRCGYC	1 cut(s)	2204
BstAFI	CTTAAG	1 cut(s)	2323
BstAPI	GCANNNNNTGC	2 cut(s)	584, 929
BstAUI	TGTACA	1 cut(s)	3604
BstBAI	YACGTR	1 cut(s)	167
BstDSI	CCRYGG	2 cut(s)	2614, 2803
BstEII	GGTNACC	2 cut(s)	2682, 3133
BstFNI	CGCG	2 cut(s)	1314, 3663
BstH2I	RGCGCY	2 cut(s)	844, 1505
BstHHI	GCGC	3 cut(s)	843, 1504, 3665
BstMAI	GTCTC	5 cut(s)	634, 682, 2211, 3070, 3201
BstNI	CCWGG	5 cut(s)	1157, 1879, 2075, 2778, 3222
BstNSI	RCATGY	2 cut(s)	897, 2041
BstPI	GGTNACC	2 cut(s)	2682, 3133
BstSCI	CCNGG	7 cut(s)	1155, 1410, 1877, 2073, 2776, 2847, 3220
BstSFI	CTRYAG	9 cut(s)	99, 852, 1179, 2032, 2220, 2490, 2569, 3428, 3648
BstUI	CGCG	2 cut(s)	1314, 3663
BstV2I	GAAGAC	1 cut(s)	5
BstX2I	RGATCY	7 cut(s)	103, 280, 377, 722, 1186, 1391, 3290
BstXI	CCANNNNNNTGG	1 cut(s)	67
BstYI	RGATCY	7 cut(s)	103, 280, 377, 722, 1186, 1391, 3290
BstZ17I	GTATAC	1 cut(s)	164
Bsu36I	CCTNAGG	2 cut(s)	1218, 2361
BsuRI	GGCC	6 cut(s)	942, 1155, 1713, 2817, 3461, 3469
BtgI	CCRYGG	2 cut(s)	2614, 2803
BtsI	GCAGTG	2 cut(s)	428, 582
BtsIMutI	CAGTG	7 cut(s)	424, 428, 582, 600, 2913, 3371, 3411
BveI	ACCTGC	4 cut(s)	914, 1613, 2695, 3011
CaiI	CAGNNNCTG	2 cut(s)	1142, 2568
CciI	TCATGA	1 cut(s)	628
CfoI	GCGC	3 cut(s)	843, 1504, 3665
Cfr10I	RCCGGY	1 cut(s)	1901
Cfr13I	GGNCC	5 cut(s)	44, 406, 1712, 2408, 3459
CseI	GACGC	5 cut(s)	688, 1037, 1320, 2627, 3669
Csp6I	GTAC	6 cut(s)	360, 2532, 2905, 3228, 3553, 3605
CviQI	GTAC	6 cut(s)	360, 2532, 2905, 3228, 3553, 3605
DraI	TTTAAA	2 cut(s)	2835, 3310
Eam1104I	CTCTTC	3 cut(s)	1218, 3244, 3629
EarI	CTCTTC	3 cut(s)	1218, 3244, 3629
EciI	GGCGGA	1 cut(s)	3027
Eco130I	CCWWGG	6 cut(s)	1150, 1240, 1697, 2614, 2803, 3473
Eco147I	AGGCCT	1 cut(s)	3469
Eco31I	GGTCTC	1 cut(s)	3070
Eco47I	GGWCC	3 cut(s)	44, 406, 2408
Eco47III	AGCGCT	1 cut(s)	1503
Eco57I	CTGAAG	4 cut(s)	201, 1072, 3028, 3650
Eco81I	CCTNAGG	2 cut(s)	1218, 2361
Eco88I	CYCGRG	1 cut(s)	2535
Eco91I	GGTNACC	2 cut(s)	2682, 3133
EcoO109I	RGGNCCY	2 cut(s)	406, 2408
EcoO65I	GGTNACC	2 cut(s)	2682, 3133
EcoRI	GAATTC	3 cut(s)	1736, 2249, 3298
EcoRII	CCWGG	5 cut(s)	1155, 1877, 2073, 2776, 3220
EcoT14I	CCWWGG	6 cut(s)	1150, 1240, 1697, 2614, 2803, 3473
EcoT22I	ATGCAT	6 cut(s)	1576, 1984, 2484, 2506, 2718, 3000
ErhI	CCWWGG	6 cut(s)	1150, 1240, 1697, 2614, 2803, 3473
Esp3I	CGTCTC	1 cut(s)	2211
FalI	AAGNNNNNCTT	4 cut(s)	289, 321, 593, 625
FaqI	GGGAC	7 cut(s)	392, 904, 1258, 1457, 2421, 2944, 3468
FauI	CCCGC	3 cut(s)	1789, 2947, 3557
FbaI	TGATCA	2 cut(s)	247, 2473
FblI	GTMKAC	1 cut(s)	163
FspBI	CTAG	4 cut(s)	828, 1505, 1910, 2631
GlaI	GCGC	3 cut(s)	842, 1503, 3664
GsaI	CCCAGC	2 cut(s)	3400, 3521
GsuI	CTGGAG	2 cut(s)	2760, 3271
HaeII	RGCGCY	2 cut(s)	844, 1505
HaeIII	GGCC	6 cut(s)	942, 1155, 1713, 2817, 3461, 3469
HapII	CCGG	3 cut(s)	1412, 1902, 2849
HgaI	GACGC	5 cut(s)	688, 1037, 1320, 2627, 3669
HhaI	GCGC	3 cut(s)	843, 1504, 3665
Hin1I	GRCGYC	1 cut(s)	2204
Hin6I	GCGC	3 cut(s)	841, 1502, 3663
HinP1I	GCGC	3 cut(s)	841, 1502, 3663
HincII	GTYRAC	4 cut(s)	667, 916, 1884, 2431
HindII	GTYRAC	4 cut(s)	667, 916, 1884, 2431
HindIII	AAGCTT	1 cut(s)	203
HpaII	CCGG	3 cut(s)	1412, 1902, 2849
HphI	GGTGA	9 cut(s)	242, 811, 938, 1079, 1205, 1770, 2020, 2676, 2819
Hpy166II	GTNNAC	5 cut(s)	164, 667, 916, 1884, 2431
Hpy8I	GTNNAC	5 cut(s)	164, 667, 916, 1884, 2431
HpyCH4III	ACNGT	6 cut(s)	419, 472, 595, 913, 1144, 1582
HpyCH4IV	ACGT	3 cut(s)	166, 2204, 2923
HpySE526I	ACGT	3 cut(s)	166, 2204, 2923
Hsp92I	GRCGYC	1 cut(s)	2204
HspAI	GCGC	3 cut(s)	841, 1502, 3663
Ksp22I	TGATCA	2 cut(s)	247, 2473
LmnI	GCTCC	8 cut(s)	253, 430, 783, 1377, 2075, 2216, 3290, 3477
MaeI	CTAG	4 cut(s)	828, 1505, 1910, 2631
MaeII	ACGT	3 cut(s)	166, 2204, 2923
MaeIII	GTNAC	4 cut(s)	2558, 2682, 2924, 3133
MflI	RGATCY	7 cut(s)	103, 280, 377, 722, 1186, 1391, 3290
MhlI	GDGCHC	2 cut(s)	403, 2581
MluI	ACGCGT	1 cut(s)	1312
MlyI	GAGTC	5 cut(s)	634, 636, 881, 1769, 3357
MmeI	TCCRAC	4 cut(s)	216, 886, 1134, 1495
Mph1103I	ATGCAT	6 cut(s)	1576, 1984, 2484, 2506, 2718, 3000
MroXI	GAANNNNTTC	1 cut(s)	1144
MslI	CAYNNNNRTG	7 cut(s)	417, 420, 593, 972, 2234, 2307, 3670
MspA1I	CMGCKG	6 cut(s)	1784, 2159, 2568, 2954, 3521, 3628
MspCI	CTTAAG	1 cut(s)	2323
MspI	CCGG	3 cut(s)	1412, 1902, 2849
MspR9I	CCNGG	7 cut(s)	1157, 1412, 1879, 2075, 2778, 2849, 3222
Mva1269I	GAATGC	2 cut(s)	886, 2504
MvaI	CCWGG	5 cut(s)	1157, 1879, 2075, 2778, 3222
MvnI	CGCG	2 cut(s)	1314, 3663
NciI	CCSGG	2 cut(s)	1412, 2849
NcoI	CCATGG	2 cut(s)	2614, 2803
NlaIV	GGNNCC	9 cut(s)	255, 282, 408, 1639, 1900, 2145, 2409, 3286, 3460
NmeAIII	GCCGAG	2 cut(s)	663, 862
NmuCI	GTSAC	1 cut(s)	2682
NsiI	ATGCAT	6 cut(s)	1576, 1984, 2484, 2506, 2718, 3000
NspI	RCATGY	2 cut(s)	897, 2041
OliI	CACNNNNGTG	1 cut(s)	417
PaeI	GCATGC	1 cut(s)	2041
PaeR7I	CTCGAG	1 cut(s)	2535
PagI	TCATGA	1 cut(s)	628
PaqCI	CACCTGC	2 cut(s)	914, 2695
PceI	AGGCCT	1 cut(s)	3469
PciI	ACATGT	1 cut(s)	893
PctI	GAATGC	2 cut(s)	886, 2504
PdmI	GAANNNNTTC	1 cut(s)	1144
PflMI	CCANNNNNTGG	1 cut(s)	3542
PleI	GAGTC	5 cut(s)	633, 636, 880, 1769, 3357
PpsI	GAGTC	5 cut(s)	633, 636, 880, 1769, 3357
Ppu21I	YACGTR	1 cut(s)	167
PpuMI	RGGWCCY	2 cut(s)	406, 2408
PscI	ACATGT	1 cut(s)	893
PshBI	ATTAAT	1 cut(s)	954
PsiI	TTATAA	1 cut(s)	3129
Psp5II	RGGWCCY	2 cut(s)	406, 2408
Psp6I	CCWGG	5 cut(s)	1155, 1877, 2073, 2776, 3220
PspEI	GGTNACC	2 cut(s)	2682, 3133
PspFI	CCCAGC	2 cut(s)	3396, 3517
PspGI	CCWGG	5 cut(s)	1155, 1877, 2073, 2776, 3220
PspN4I	GGNNCC	9 cut(s)	255, 282, 408, 1639, 1900, 2145, 2409, 3286, 3460
PspPI	GGNCC	5 cut(s)	44, 406, 1712, 2408, 3459
PspPPI	RGGWCCY	2 cut(s)	406, 2408
PsrI	GAACNNNNNNTAC	2 cut(s)	168, 200
PstI	CTGCAG	3 cut(s)	856, 2224, 3432
PstNI	CAGNNNCTG	2 cut(s)	1142, 2568
PsuI	RGATCY	7 cut(s)	103, 280, 377, 722, 1186, 1391, 3290
PvuII	CAGCTG	4 cut(s)	2159, 2568, 3521, 3628
RsaI	GTAC	6 cut(s)	361, 2533, 2906, 3229, 3554, 3606
RsaNI	GTAC	6 cut(s)	360, 2532, 2905, 3228, 3553, 3605
RseI	CAYNNNNRTG	7 cut(s)	417, 420, 593, 972, 2234, 2307, 3670
Sau96I	GGNCC	5 cut(s)	44, 406, 1712, 2408, 3459
ScaI	AGTACT	2 cut(s)	2533, 2906
SchI	GAGTC	5 cut(s)	634, 636, 881, 1769, 3357
ScrFI	CCNGG	7 cut(s)	1157, 1412, 1879, 2075, 2778, 2849, 3222
SduI	GDGCHC	2 cut(s)	403, 2581
SfcI	CTRYAG	9 cut(s)	99, 852, 1179, 2032, 2220, 2490, 2569, 3428, 3648
Sfr274I	CTCGAG	1 cut(s)	2535
SinI	GGWCC	3 cut(s)	44, 406, 2408
SlaI	CTCGAG	1 cut(s)	2535
SmiI	ATTTAAAT	1 cut(s)	2835
SmiMI	CAYNNNNRTG	7 cut(s)	417, 420, 593, 972, 2234, 2307, 3670
SmlI	CTYRAG	2 cut(s)	2323, 2535
SmoI	CTYRAG	2 cut(s)	2323, 2535
SpeI	ACTAGT	1 cut(s)	827
SphI	GCATGC	1 cut(s)	2041
SseBI	AGGCCT	1 cut(s)	3469
SsiI	CCGC	9 cut(s)	1648, 1782, 1934, 2954, 3028, 3038, 3110, 3171, 3564
SspI	AATATT	1 cut(s)	2331
SspMI	CTAG	4 cut(s)	828, 1505, 1910, 2631
StuI	AGGCCT	1 cut(s)	3469
StyD4I	CCNGG	7 cut(s)	1155, 1410, 1877, 2073, 2776, 2847, 3220
StyI	CCWWGG	6 cut(s)	1150, 1240, 1697, 2614, 2803, 3473
SwaI	ATTTAAAT	1 cut(s)	2835
TaaI	ACNGT	6 cut(s)	419, 472, 595, 913, 1144, 1582
TaiI	ACGT	3 cut(s)	169, 2207, 2926
TaqI	TCGA	4 cut(s)	21, 2536, 2757, 3095
TatI	WGTACW	4 cut(s)	2531, 2904, 3552, 3604
TauI	GCSGC	1 cut(s)	3174
TscAI	CASTG	7 cut(s)	424, 435, 589, 600, 2920, 3378, 3418
TseFI	GTSAC	1 cut(s)	2682
Tsp45I	GTSAC	1 cut(s)	2682
TspGWI	ACGGA	2 cut(s)	1109, 2631
TspRI	CASTG	7 cut(s)	424, 435, 589, 600, 2920, 3378, 3418
Van91I	CCANNNNNTGG	1 cut(s)	3542
Vha464I	CTTAAG	1 cut(s)	2323
VpaK11BI	GGWCC	3 cut(s)	44, 406, 2408
VspI	ATTAAT	1 cut(s)	954
XceI	RCATGY	2 cut(s)	897, 2041
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2810
XhoI	CTCGAG	1 cut(s)	2535
XmiI	GTMKAC	1 cut(s)	163
XmnI	GAANNNNTTC	1 cut(s)	1144
XspI	CTAG	4 cut(s)	828, 1505, 1910, 2631
ZraI	GACGTC	1 cut(s)	2205
ZrmI	AGTACT	2 cut(s)	2533, 2906
Zsp2I	ATGCAT	6 cut(s)	1576, 1984, 2484, 2506, 2718, 3000

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.