Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0012478

Physical Location & Seq

Genomic Coordinates Reverse (-)

6682537 .. 6688287

Gene Structure 5751 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1096000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3192 bp

ATGGAGAAGGGGCTTCTGATCGACAATAATCATAAGAGGAGCGATCCTATTATTGCCTCCAAGTCGGTGGGATCGTTTGTGAAAATTGATTCAATATCTATTGATCTTCCTGATGCGAATGACAAGAGTAGTAGCCATGCGGGAAACTGCGAGCATTTCACTATATGTGGTTATGTAGCTGAAGTTCGCAAAAAGAATTGGAAGCTATGTTGGCCATTTCCTTTGGATGGTGAGTTTGATCAGGAGCCAACATCCTTGCTTCCTCCCTTGGATCCTCCAAAATTCAGAAGGTGGTCTTGCCAGAATTGTGTGCAGGAAACTGGTTCTGAGAGTATTGCAAAAGATCATGGTACGGACATGCGTATAGGATCTGATTCCAATAACAATTGTGCTCAGATCCCACCCCTCAGTGATGCTTCACTGCTTCTGTTAGATTATGAGAAAGGTTCAATTTCCAAAATTGTTGATGGAAAAAGTGCTGAAGCTCATATTTCTGTTGTTGGAATTGGCAATGAGCAACATCCTTCGTCATCTACTTTTGATAAGAAAGAAGGGAGGCATGAAGTTTCACTCACTGCCATCACAGTGAATGAGAATGTCTTAGATAATTATGCGAGTCATGAAATGCCGAGACTCAATTCTGTTGGGCCAGAAGTTGACCCAAAAAGGACGCAGGAGGGACATAAAGATAACACAGAAGCAGTAAATTTGGAGAGCAATGGATCTGTTGAAGTCTGGAAATTAGGATGTGAAAGTCGTGGTGGTACCAACCTTCAACTCCATTTCTCAAACAAAAAATTATTCTGGAATAAGAATCTTGCCCAGACTAGTGAAGCTGATCACCGACCTTCTGCAGAAGAAAATCACAAGGAGTCAATGACTGCCTCTGGGACATGTAAGATAGTCGGGACGGTTGACAGGGTAAGTGATGCTACCAAGGGCCAGACCATCATTAATCATCCTCTGCATGAGTGTGATTATGCATCATCGGAAAATCCTGAGAAGTCGAGTAGTTTGAATCGTAAGAAAGGTCGGAAGGTGCGTGTGCGTCTGCTGAGTGAGTTGGGTGATGCAAAGCCTGATTGCTTCAGGACAGAAGATTCTCCATCCAACAGAATTAGCAATACATCTGGGAAAGCAACTGTTTCCCAAGGCCAGGTGTCTATCCAAGAAAGTGCTAGAGTGGATCTCGGTGAGAATAACAAAAGGAAATTGCCTGAGGAAGAGGAACAGAGATGCATAGAGAAGAGCCATCCTAAAAGTTTGAATAGAAAAGTTAAGACTTCCAAGCGAGATGAAGAAACCACCGGAAGCGTTGCAGATTCTGAGTTAGAAGAAGATGCAGTTGCCAGAGTATCTTTGCAGGCAGATATGAGGAGCAACTGGAATGGATCCGAAAATGAAAGAATCCCGGCTGCTGGTAAGAAGAAAATTATGAAATCCAATGATATTGGCGCATGTTCATCCTTAGTCCCACCCAAAGATATTGTGCCAATAGAAGCCCTAGACAAAGTTGGAAATTCAAGTAAGGGTAATGCTACTACAAGTGCTTCTTATAGTCCAGCGTATGATGCATCCACAGGTAGAGGGATGGATCAAATTGCTTTGCCTGCCCCAAAAGCAGGTAGAAAGTCCGGTTCCTGTAAGCGGAAAGACAAGATGCCGGAAGTTGATAACGGACTGGCTTCTCTATTTCCTTGGCTCACGAAATGCCCAAATACAAGGAAAGATGTAGAATTCATGCAAACTGGGAATCCATTTCACTGGGCAGAAGATGCACCCGCTGAAAAAGGGTTGGATCTTTCTCTTAACAGCTATTTGTCTGCACAAAGAGATGACAGGAAATCTATTCCTCAGTCTGAAGATGGATTTCCTTCCTGGTCAATTCAGAAAGATGGAACCGGCAAACTAGATGAGTTTATTAGGAAAAATGCAGAAGCCAGCGCAAATCTGACCAAACTTCCTAAAGCCATTTCAAATGCATTTTCTGGGAAAGTAGGACATGATGATCTGAGTAGTAAATTTTTCACCTACAGCATGCCTATCCCGAATGAGAAGCAAAACTATAGCCCCCAGGTTGAAGGGAGATGTTTGTTGCAGCATATGGATATTTCTCGTGCAAGAAACAAGGAGAAAATTGAGGTTCCGAAAAATTCAGCTGTGCCTGTTAGTAGGAAAGATAGCAAGCAAAGAGCTGAAAAGACGTCTCAGAAGGGAGCTGCAGATGACATACCGATGGAAATTGTTGAATTCATGGCGAAAAATCATTATGAGAGAAATCAGAAAGTTTTGGAAGCAGGCATTAATGCAAGGAATGTTCAGGCGATGGATCATAGTTATGGCATTGGGAACTTAAGAATATTAGAGGAAACCAGTCAGAAGCGAAAACCTCAGGCAAGAAAGGCAAAAGATGGTGCAACCGCAAGAAAAAATGTGGGACCTTCCAAACAGAAGTTAGTTGACTTTTCTACTTATATCCCCGGAAAAGACATATTCCGTCTCGATCAAATGCATTGCGCTGTAGGGTTTAATGCATTTGGTCAACCTAAAAAGAAGATGAGTACTCGAGAGCAATTTCCTGCTGCTGGTTACAGCAGCTGTAGTTGTGCCCAAAATTGCAAATGGAATGGAGATATGATGAGCGATGGGTTGTCAAATTCTAGCTTGCGTCCGTCTGTTCCACAATCAAAGGAAGACGCAGCTCTCCTATGGTCACCTGCGTTGCCAGCTCAAACAGCCTTAACACACTACAATCCTCTTAAGTATGGAGCTCAGCCTTCCAATGTCGATATGCTTTCACACCCTCCTGGCTCGCTGCATAAGGGAAACTTCCGTGGGGATTATGGCCTGAAATTATTTAATTTAAATGCTACCAACCCGGAAAAGCATAGTGAAGGTGTTGGTTCTGAAACTTTCAGCAGAACAAATGCAGAGTACTCATTCACTGGTCAACGTAATGCGACCGAGCCTCCTCAGGGTTCAGCGGGTTCTTTTGATCTATATTCTAATGAAACCATACCAGCAATGCATTTGCTCAGCCTCATGGATGCAGGTATGCGGTCAGGTCCAACCTTGAACATTGCTGGAAACCCAAAATTTCCCAAGAGGTGGCTGATACCGTCAAGCATCCATCATCCAGTTTTTGCGGCAAAAATCACATTTCTGAGAAGTCTCTTGACCTTTTTCCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008213 GO:0009628 GO:0009651 GO:0009791 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010022 GO:0010073 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032501 GO:0032502 GO:0034968 GO:0036211 GO:0042538 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048367 GO:0048507 GO:0048519 GO:0048523 GO:0048580 GO:0048581 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0065007 GO:0070734 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

1064

Amino Acids

116.88

Weight (kDa)

8.45

Isoelectric Point (pI)

44.84

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012478)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G11530 AT5G11530 AT5G11530
fragaria_vesca	FvH4_2g35880 FvH4_2g35880
malus_domestica	MD08G1116300.v1.1 MD15G1096000.v1.1
prunus_persica	Prupe.1G450900_v2.0.a1
pyrus_communis	pycom08g09700
rosa_chinensis	RchiOBHm_Chr6g0305941
rosa_laevigata	RLG00000010844
rosa_multiflora	Rmu_sc0001809.1_g000052
rosa_roxburghii	Rroxscaffold_7G00162200
rosa_rugosa	Rorug06G0345500
rosa_samantha	Rh6AG457200 Rh6BG443900 Rh6CG471200 Rh6DG457700
rosa_wichuraiana	Rw6G039890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2725
AatII	GACGTC	1 cut(s)	2207
Acc36I	ACCTGC	3 cut(s)	1613, 2725, 3041
Acc65I	GGTACC	1 cut(s)	764
AccB1I	GGYRCC	1 cut(s)	764
AccB7I	CCANNNNNTGG	1 cut(s)	3108
AciI	CCGC	7 cut(s)	140, 1648, 1782, 2421, 2984, 3058, 3146
AcoI	YGGCCR	1 cut(s)	212
AcsI	RAATTY	9 cut(s)	281, 706, 1519, 1736, 2021, 2152, 2249, 2656, 3095
AcuI	CTGAAG	4 cut(s)	201, 501, 1072, 1881
AcyI	GRCGYC	1 cut(s)	2204
AfaI	GTAC	4 cut(s)	352, 766, 2563, 2936
AfiI	CCNNNNNNNGG	8 cut(s)	227, 1156, 1418, 1622, 1647, 2585, 2975, 3108
AflII	CTTAAG	2 cut(s)	2353, 2759
AflIII	ACRYGT	1 cut(s)	893
AhlI	ACTAGT	1 cut(s)	827
AjnI	CCWGG	4 cut(s)	1155, 1877, 2073, 2806
Alw21I	GWGCWC	2 cut(s)	394, 2773
Alw26I	GTCTC	4 cut(s)	625, 2211, 2504, 3176
AlwNI	CAGNNNCTG	2 cut(s)	1325, 2598
Ama87I	CYCGRG	1 cut(s)	2565
AoxI	GGCC	5 cut(s)	212, 647, 940, 1153, 2845
ApeKI	GCWGC	7 cut(s)	1415, 2098, 2219, 2582, 2595, 2699, 2815
ApoI	RAATTY	9 cut(s)	281, 706, 1519, 1736, 2021, 2152, 2249, 2656, 3095
AseI	ATTAAT	2 cut(s)	954, 2304
Asp718I	GGTACC	1 cut(s)	764
AspLEI	GCGC	3 cut(s)	1457, 1946, 2519
AspS9I	GGNCC	4 cut(s)	647, 940, 2438, 3065
AsuC2I	CCSGG	3 cut(s)	1412, 2481, 2879
AsuHPI	GGTGA	6 cut(s)	242, 833, 1079, 1205, 2020, 2706
AvaI	CYCGRG	1 cut(s)	2565
AvaII	GGWCC	2 cut(s)	2438, 3065
AxyI	CCTNAGG	3 cut(s)	1218, 2391, 2973
BaeGI	GKGCMC	1 cut(s)	2611
BalI	TGGCCA	1 cut(s)	214
BamHI	GGATCC	2 cut(s)	271, 1391
BanI	GGYRCC	1 cut(s)	764
BanII	GRGCYC	1 cut(s)	2773
BauI	CACGAG	1 cut(s)	2115
BbsI	GAAGAC	1 cut(s)	2700
Bbv12I	GWGCWC	2 cut(s)	394, 2773
BbvI	GCAGC	7 cut(s)	1402, 2110, 2206, 2569, 2607, 2711, 2802
BciT130I	CCWGG	4 cut(s)	1157, 1879, 2075, 2808
BclI	TGATCA	2 cut(s)	238, 838
BcnI	CCSGG	3 cut(s)	1412, 2481, 2879
BcoDI	GTCTC	4 cut(s)	625, 2211, 2504, 3176
BcuI	ACTAGT	1 cut(s)	827
BfaI	CTAG	5 cut(s)	828, 1179, 1505, 1910, 2661
BfmI	CTRYAG	6 cut(s)	852, 2032, 2065, 2220, 2520, 2599
BfrI	CTTAAG	2 cut(s)	2353, 2759
BfuAI	ACCTGC	3 cut(s)	1613, 2725, 3041
BisI	GCNGC	8 cut(s)	1416, 2099, 2220, 2583, 2596, 2700, 2816, 3147
BlpI	GCTNAGC	2 cut(s)	2772, 3035
BlsI	GCNGC	8 cut(s)	1417, 2100, 2221, 2584, 2597, 2701, 2817, 3148
BmcAI	AGTACT	2 cut(s)	2563, 2936
Bme1390I	CCNGG	7 cut(s)	1157, 1412, 1879, 2075, 2481, 2808, 2879
Bme18I	GGWCC	2 cut(s)	2438, 3065
BmeT110I	CYCGRG	1 cut(s)	2565
BmgT120I	GGNCC	4 cut(s)	647, 940, 2438, 3065
BmiI	GGNNCC	8 cut(s)	246, 273, 766, 1393, 1639, 1900, 2145, 2439
BmrFI	CCNGG	7 cut(s)	1157, 1412, 1879, 2075, 2481, 2808, 2879
BmrI	ACTGGG	2 cut(s)	1758, 1774
BmuI	ACTGGG	2 cut(s)	1758, 1774
BpiI	GAAGAC	1 cut(s)	2700
BplI	GAGNNNNNCTC	2 cut(s)	1173, 1205
Bpu1102I	GCTNAGC	2 cut(s)	2772, 3035
BpuMI	CCSGG	3 cut(s)	1412, 2481, 2879
BsaHI	GRCGYC	1 cut(s)	2204
BsaJI	CCNNGG	7 cut(s)	267, 936, 1150, 1697, 2073, 2479, 2833
BsaWI	WCCGGW	2 cut(s)	1307, 1634
BsaXI	ACNNNNNCTCC	2 cut(s)	2953, 2983
Bsc4I	CCNNNNNNNGG	8 cut(s)	227, 1156, 1418, 1622, 1647, 2585, 2975, 3108
Bse118I	RCCGGY	1 cut(s)	1901
Bse1I	ACTGG	8 cut(s)	325, 1388, 1686, 1753, 1769, 2373, 2950, 3137
Bse21I	CCTNAGG	3 cut(s)	1218, 2391, 2973
Bse3DI	GCAATG	5 cut(s)	517, 724, 2512, 3030, 3078
BseBI	CCWGG	4 cut(s)	1157, 1879, 2075, 2808
BseDI	CCNNGG	7 cut(s)	267, 936, 1150, 1697, 2073, 2479, 2833
BseLI	CCNNNNNNNGG	8 cut(s)	227, 1156, 1418, 1622, 1647, 2585, 2975, 3108
BseMI	GCAATG	5 cut(s)	517, 724, 2512, 3030, 3078
BseNI	ACTGG	8 cut(s)	325, 1388, 1686, 1753, 1769, 2373, 2950, 3137
BseRI	GAGGAG	3 cut(s)	52, 1390, 2961
BseSI	GKGCMC	1 cut(s)	2611
BseXI	GCAGC	7 cut(s)	1402, 2110, 2206, 2569, 2607, 2711, 2802
BsgI	GTGCAG	2 cut(s)	332, 1809
Bsh1285I	CGRYCG	1 cut(s)	2964
BshFI	GGCC	5 cut(s)	214, 649, 942, 1155, 2847
BshNI	GGYRCC	1 cut(s)	764
BsiEI	CGRYCG	1 cut(s)	2964
BsiHKAI	GWGCWC	2 cut(s)	394, 2773
BsiHKCI	CYCGRG	1 cut(s)	2565
BsiSI	CCGG	7 cut(s)	1308, 1412, 1635, 1664, 1902, 2481, 2879
BslFI	GGGAC	5 cut(s)	693, 904, 922, 1457, 2451
BslI	CCNNNNNNNGG	8 cut(s)	227, 1156, 1418, 1622, 1647, 2585, 2975, 3108
BsmAI	GTCTC	4 cut(s)	625, 2211, 2504, 3176
BsmBI	CGTCTC	2 cut(s)	2211, 2504
BsmFI	GGGAC	5 cut(s)	693, 904, 922, 1457, 2451
BsnI	GGCC	5 cut(s)	214, 649, 942, 1155, 2847
BsoBI	CYCGRG	1 cut(s)	2565
Bsp1286I	GDGCHC	3 cut(s)	394, 2611, 2773
Bsp1720I	GCTNAGC	2 cut(s)	2772, 3035
BspACI	CCGC	7 cut(s)	140, 1648, 1782, 2421, 2984, 3058, 3146
BspANI	GGCC	5 cut(s)	214, 649, 942, 1155, 2847
BspHI	TCATGA	1 cut(s)	619
BspLI	GGNNCC	8 cut(s)	246, 273, 766, 1393, 1639, 1900, 2145, 2439
BspMAI	CTGCAG	2 cut(s)	856, 2224
BspMI	ACCTGC	3 cut(s)	1613, 2725, 3041
BspQI	GCTCTTC	1 cut(s)	1241
BspT107I	GGYRCC	1 cut(s)	764
BspTI	CTTAAG	2 cut(s)	2353, 2759
BsrDI	GCAATG	5 cut(s)	517, 724, 2512, 3030, 3078
BsrFI	RCCGGY	1 cut(s)	1901
BsrI	ACTGG	8 cut(s)	325, 1388, 1686, 1753, 1769, 2373, 2950, 3137
BssAI	RCCGGY	1 cut(s)	1901
BssECI	CCNNGG	7 cut(s)	267, 936, 1150, 1697, 2073, 2479, 2833
BssNI	GRCGYC	1 cut(s)	2204
BssSI	CACGAG	1 cut(s)	2115
BssT1I	CCWWGG	4 cut(s)	267, 936, 1150, 1697
Bst2BI	CACGAG	1 cut(s)	2115
Bst2UI	CCWGG	4 cut(s)	1157, 1879, 2075, 2808
Bst4CI	ACNGT	4 cut(s)	586, 913, 1144, 3120
Bst6I	CTCTTC	2 cut(s)	1218, 1241
BstACI	GRCGYC	1 cut(s)	2204
BstAFI	CTTAAG	2 cut(s)	2353, 2759
BstAPI	GCANNNNNTGC	1 cut(s)	1775
BstDSI	CCRYGG	1 cut(s)	2833
BstEII	GGTNACC	1 cut(s)	2712
BstHHI	GCGC	3 cut(s)	1457, 1946, 2519
BstMAI	GTCTC	4 cut(s)	625, 2211, 2504, 3176
BstMCI	CGRYCG	1 cut(s)	2964
BstMWI	GCNNNNNNNGC	8 cut(s)	211, 1571, 1610, 1619, 1775, 2402, 2726, 2735
BstNI	CCWGG	4 cut(s)	1157, 1879, 2075, 2808
BstNSI	RCATGY	4 cut(s)	361, 897, 1461, 2041
BstPI	GGTNACC	1 cut(s)	2712
BstSCI	CCNGG	7 cut(s)	1155, 1410, 1877, 2073, 2479, 2806, 2877
BstSFI	CTRYAG	6 cut(s)	852, 2032, 2065, 2220, 2520, 2599
BstSLI	GKGCMC	1 cut(s)	2611
BstV1I	GCAGC	7 cut(s)	1402, 2110, 2206, 2569, 2607, 2711, 2802
BstV2I	GAAGAC	1 cut(s)	2700
BstX2I	RGATCY	7 cut(s)	271, 368, 396, 722, 1186, 1391, 1798
BstXI	CCANNNNNNTGG	2 cut(s)	67, 1764
BstYI	RGATCY	7 cut(s)	271, 368, 396, 722, 1186, 1391, 1798
Bsu36I	CCTNAGG	3 cut(s)	1218, 2391, 2973
BsuRI	GGCC	5 cut(s)	214, 649, 942, 1155, 2847
BtgI	CCRYGG	1 cut(s)	2833
BtgZI	GCGATG	2 cut(s)	2339, 2658
BtsI	GCAGTG	2 cut(s)	419, 573
BtsIMutI	CAGTG	6 cut(s)	415, 419, 573, 591, 1762, 2943
BveI	ACCTGC	3 cut(s)	1613, 2725, 3041
CaiI	CAGNNNCTG	2 cut(s)	1325, 2598
CciI	TCATGA	1 cut(s)	619
CfoI	GCGC	3 cut(s)	1457, 1946, 2519
Cfr10I	RCCGGY	1 cut(s)	1901
Cfr13I	GGNCC	4 cut(s)	647, 940, 2438, 3065
CseI	GACGC	4 cut(s)	679, 1037, 2657, 2705
Csp6I	GTAC	4 cut(s)	351, 765, 2562, 2935
CviQI	GTAC	4 cut(s)	351, 765, 2562, 2935
DraI	TTTAAA	1 cut(s)	2865
EaeI	YGGCCR	1 cut(s)	212
Eam1104I	CTCTTC	2 cut(s)	1218, 1241
EarI	CTCTTC	2 cut(s)	1218, 1241
Ecl136II	GAGCTC	1 cut(s)	2771
Eco130I	CCWWGG	4 cut(s)	267, 936, 1150, 1697
Eco24I	GRGCYC	1 cut(s)	2773
Eco47I	GGWCC	2 cut(s)	2438, 3065
Eco53kI	GAGCTC	1 cut(s)	2771
Eco57I	CTGAAG	4 cut(s)	201, 501, 1072, 1881
Eco81I	CCTNAGG	3 cut(s)	1218, 2391, 2973
Eco88I	CYCGRG	1 cut(s)	2565
Eco91I	GGTNACC	1 cut(s)	2712
EcoICRI	GAGCTC	1 cut(s)	2771
EcoO109I	RGGNCCY	1 cut(s)	2438
EcoO65I	GGTNACC	1 cut(s)	2712
EcoRI	GAATTC	2 cut(s)	1736, 2249
EcoRII	CCWGG	4 cut(s)	1155, 1877, 2073, 2806
EcoT14I	CCWWGG	4 cut(s)	267, 936, 1150, 1697
EcoT22I	ATGCAT	7 cut(s)	985, 1241, 1576, 1984, 2514, 2536, 3030
EcoT38I	GRGCYC	1 cut(s)	2773
ErhI	CCWWGG	4 cut(s)	267, 936, 1150, 1697
Esp3I	CGTCTC	2 cut(s)	2211, 2504
FalI	AAGNNNNNCTT	6 cut(s)	280, 312, 1537, 1569, 2813, 2845
FaqI	GGGAC	5 cut(s)	693, 904, 922, 1457, 2451
FauI	CCCGC	3 cut(s)	133, 1789, 2977
FauNDI	CATATG	1 cut(s)	2103
FbaI	TGATCA	2 cut(s)	238, 838
Fnu4HI	GCNGC	8 cut(s)	1416, 2099, 2220, 2583, 2596, 2700, 2816, 3147
FriOI	GRGCYC	1 cut(s)	2773
Fsp4HI	GCNGC	8 cut(s)	1416, 2099, 2220, 2583, 2596, 2700, 2816, 3147
FspBI	CTAG	5 cut(s)	828, 1179, 1505, 1910, 2661
GlaI	GCGC	3 cut(s)	1456, 1945, 2518
GluI	GCNGC	8 cut(s)	1416, 2099, 2220, 2583, 2596, 2700, 2816, 3147
HaeIII	GGCC	5 cut(s)	214, 649, 942, 1155, 2847
HapII	CCGG	7 cut(s)	1308, 1412, 1635, 1664, 1902, 2481, 2879
HgaI	GACGC	4 cut(s)	679, 1037, 2657, 2705
HhaI	GCGC	3 cut(s)	1457, 1946, 2519
Hin1I	GRCGYC	1 cut(s)	2204
Hin6I	GCGC	3 cut(s)	1455, 1944, 2517
HinP1I	GCGC	3 cut(s)	1455, 1944, 2517
HincII	GTYRAC	5 cut(s)	658, 916, 2461, 2543, 2951
HindII	GTYRAC	5 cut(s)	658, 916, 2461, 2543, 2951
HpaII	CCGG	7 cut(s)	1308, 1412, 1635, 1664, 1902, 2481, 2879
HphI	GGTGA	6 cut(s)	242, 833, 1079, 1205, 2020, 2706
Hpy166II	GTNNAC	5 cut(s)	658, 916, 2461, 2543, 2951
Hpy8I	GTNNAC	5 cut(s)	658, 916, 2461, 2543, 2951
HpyCH4III	ACNGT	4 cut(s)	586, 913, 1144, 3120
HpyCH4IV	ACGT	2 cut(s)	2204, 2953
HpyF10VI	GCNNNNNNNGC	8 cut(s)	211, 1571, 1610, 1619, 1775, 2402, 2726, 2735
HpySE526I	ACGT	2 cut(s)	2204, 2953
Hsp92I	GRCGYC	1 cut(s)	2204
HspAI	GCGC	3 cut(s)	1455, 1944, 2517
KpnI	GGTACC	1 cut(s)	768
Ksp22I	TGATCA	2 cut(s)	238, 838
LguI	GCTCTTC	1 cut(s)	1241
LmnI	GCTCC	5 cut(s)	39, 244, 1377, 2216, 2768
Lsp1109I	GCAGC	7 cut(s)	1402, 2110, 2206, 2569, 2607, 2711, 2802
MaeI	CTAG	5 cut(s)	828, 1179, 1505, 1910, 2661
MaeII	ACGT	2 cut(s)	2204, 2953
MaeIII	GTNAC	2 cut(s)	2588, 2712
MfeI	CAATTG	1 cut(s)	385
MflI	RGATCY	7 cut(s)	271, 368, 396, 722, 1186, 1391, 1798
MhlI	GDGCHC	3 cut(s)	394, 2611, 2773
MlsI	TGGCCA	1 cut(s)	214
MluNI	TGGCCA	1 cut(s)	214
MlyI	GAGTC	3 cut(s)	625, 627, 881
MmeI	TCCRAC	6 cut(s)	481, 1013, 1134, 1495, 1776, 3092
Mox20I	TGGCCA	1 cut(s)	214
Mph1103I	ATGCAT	7 cut(s)	985, 1241, 1576, 1984, 2514, 2536, 3030
MscI	TGGCCA	1 cut(s)	214
MslI	CAYNNNNRTG	4 cut(s)	584, 972, 2234, 2337
Msp20I	TGGCCA	1 cut(s)	214
MspA1I	CMGCKG	4 cut(s)	1784, 2159, 2598, 2984
MspCI	CTTAAG	2 cut(s)	2353, 2759
MspI	CCGG	7 cut(s)	1308, 1412, 1635, 1664, 1902, 2481, 2879
MspR9I	CCNGG	7 cut(s)	1157, 1412, 1879, 2075, 2481, 2808, 2879
MunI	CAATTG	1 cut(s)	385
MvaI	CCWGG	4 cut(s)	1157, 1879, 2075, 2808
MwoI	GCNNNNNNNGC	8 cut(s)	211, 1571, 1610, 1619, 1775, 2402, 2726, 2735
NciI	CCSGG	3 cut(s)	1412, 2481, 2879
NdeI	CATATG	1 cut(s)	2103
NlaIV	GGNNCC	8 cut(s)	246, 273, 766, 1393, 1639, 1900, 2145, 2439
NmeAIII	GCCGAG	1 cut(s)	654
NmuCI	GTSAC	1 cut(s)	2712
NsiI	ATGCAT	7 cut(s)	985, 1241, 1576, 1984, 2514, 2536, 3030
NspI	RCATGY	4 cut(s)	361, 897, 1461, 2041
PaeI	GCATGC	1 cut(s)	2041
PaeR7I	CTCGAG	1 cut(s)	2565
PagI	TCATGA	1 cut(s)	619
PaqCI	CACCTGC	1 cut(s)	2725
PciI	ACATGT	1 cut(s)	893
PciSI	GCTCTTC	1 cut(s)	1241
PcsI	WCGNNNNNNNCGW	2 cut(s)	71, 1039
PfeI	GAWTC	8 cut(s)	89, 374, 814, 1018, 1100, 1322, 1407, 1753
PflMI	CCANNNNNTGG	1 cut(s)	3108
PkrI	GCNGC	8 cut(s)	1417, 2100, 2221, 2584, 2597, 2701, 2817, 3148
PleI	GAGTC	3 cut(s)	624, 627, 880
PpsI	GAGTC	3 cut(s)	624, 627, 880
PpuMI	RGGWCCY	1 cut(s)	2438
PscI	ACATGT	1 cut(s)	893
PshBI	ATTAAT	2 cut(s)	954, 2304
Psp124BI	GAGCTC	1 cut(s)	2773
Psp5II	RGGWCCY	1 cut(s)	2438
Psp6I	CCWGG	4 cut(s)	1155, 1877, 2073, 2806
PspEI	GGTNACC	1 cut(s)	2712
PspGI	CCWGG	4 cut(s)	1155, 1877, 2073, 2806
PspN4I	GGNNCC	8 cut(s)	246, 273, 766, 1393, 1639, 1900, 2145, 2439
PspPI	GGNCC	4 cut(s)	647, 940, 2438, 3065
PspPPI	RGGWCCY	1 cut(s)	2438
PsrI	GAACNNNNNNTAC	2 cut(s)	168, 200
PstI	CTGCAG	2 cut(s)	856, 2224
PstNI	CAGNNNCTG	2 cut(s)	1325, 2598
PsuI	RGATCY	7 cut(s)	271, 368, 396, 722, 1186, 1391, 1798
PvuII	CAGCTG	2 cut(s)	2159, 2598
RsaI	GTAC	4 cut(s)	352, 766, 2563, 2936
RsaNI	GTAC	4 cut(s)	351, 765, 2562, 2935
RseI	CAYNNNNRTG	4 cut(s)	584, 972, 2234, 2337
SacI	GAGCTC	1 cut(s)	2773
SapI	GCTCTTC	1 cut(s)	1241
SatI	GCNGC	8 cut(s)	1416, 2099, 2220, 2583, 2596, 2700, 2816, 3147
Sau96I	GGNCC	4 cut(s)	647, 940, 2438, 3065
ScaI	AGTACT	2 cut(s)	2563, 2936
SchI	GAGTC	3 cut(s)	625, 627, 881
ScrFI	CCNGG	7 cut(s)	1157, 1412, 1879, 2075, 2481, 2808, 2879
SduI	GDGCHC	3 cut(s)	394, 2611, 2773
SfcI	CTRYAG	6 cut(s)	852, 2032, 2065, 2220, 2520, 2599
Sfr274I	CTCGAG	1 cut(s)	2565
SinI	GGWCC	2 cut(s)	2438, 3065
SlaI	CTCGAG	1 cut(s)	2565
SmiI	ATTTAAAT	1 cut(s)	2865
SmiMI	CAYNNNNRTG	4 cut(s)	584, 972, 2234, 2337
SmlI	CTYRAG	3 cut(s)	2353, 2565, 2759
SmoI	CTYRAG	3 cut(s)	2353, 2565, 2759
SpeI	ACTAGT	1 cut(s)	827
SphI	GCATGC	1 cut(s)	2041
SsiI	CCGC	7 cut(s)	140, 1648, 1782, 2421, 2984, 3058, 3146
SspI	AATATT	1 cut(s)	2361
SspMI	CTAG	5 cut(s)	828, 1179, 1505, 1910, 2661
SstI	GAGCTC	1 cut(s)	2773
StyD4I	CCNGG	7 cut(s)	1155, 1410, 1877, 2073, 2479, 2806, 2877
StyI	CCWWGG	4 cut(s)	267, 936, 1150, 1697
SwaI	ATTTAAAT	1 cut(s)	2865
TaaI	ACNGT	4 cut(s)	586, 913, 1144, 3120
TaiI	ACGT	2 cut(s)	2207, 2956
TaqI	TCGA	5 cut(s)	21, 1007, 2502, 2566, 2787
TaqII	GACCGA	1 cut(s)	2978
TatI	WGTACW	2 cut(s)	2561, 2934
TauI	GCSGC	1 cut(s)	3149
TfiI	GAWTC	8 cut(s)	89, 374, 814, 1018, 1100, 1322, 1407, 1753
TscAI	CASTG	6 cut(s)	415, 426, 580, 591, 1769, 2950
TseFI	GTSAC	1 cut(s)	2712
TseI	GCWGC	7 cut(s)	1415, 2098, 2219, 2582, 2595, 2699, 2815
Tsp45I	GTSAC	1 cut(s)	2712
TspDTI	ATGAA	9 cut(s)	576, 636, 1311, 1416, 1451, 1452, 1729, 2242, 3024
TspGWI	ACGGA	5 cut(s)	368, 1692, 2486, 2661, 2822
TspRI	CASTG	6 cut(s)	415, 426, 580, 591, 1769, 2950
Van91I	CCANNNNNTGG	1 cut(s)	3108
Vha464I	CTTAAG	2 cut(s)	2353, 2759
VpaK11BI	GGWCC	2 cut(s)	2438, 3065
VspI	ATTAAT	2 cut(s)	954, 2304
XapI	RAATTY	9 cut(s)	281, 706, 1519, 1736, 2021, 2152, 2249, 2656, 3095
XceI	RCATGY	4 cut(s)	361, 897, 1461, 2041
XhoI	CTCGAG	1 cut(s)	2565
XspI	CTAG	5 cut(s)	828, 1179, 1505, 1910, 2661
ZraI	GACGTC	1 cut(s)	2205
ZrmI	AGTACT	2 cut(s)	2563, 2936
Zsp2I	ATGCAT	7 cut(s)	985, 1241, 1576, 1984, 2514, 2536, 3030

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.