MD08G1128200.v1.1

Cotton fibre expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006388
Physical Location & Seq
Forward (+)
12029477 .. 12029875
399 bp
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UTR
Exon/CDS
Intron
MD08G1128200.v1.1.491

Sequence Viewer

Length: 399 bp
ATGGCTAGGGAAACCTTGTCACTCGTAAGCCACCACCTCAAGAGGGCGGTGAAGAAAATCAACCTGATTTTACTCAGCTTCAAATTAAACCGATTGCGGTTAGCTTCGATCCTTGGTTGCGCTTCTCGAGCTTCTACGCGGCATTGTTTGAGCTTCAATGACCGGTTGGGGCTTTACAGCTGCATTGAAGACGAAAATACTCATGGGAATCAGCGCGGTTTGAGAAGGGTTCAAAGCACGAGGACCCATGATCGTCCGAGGGATCAGGATTCGGACGATCACGATGACGATGATGTCAATCGCAGGGCTGATGTTTTTATAGCAAATTTTCGCCGGCAGCTGATGTTTGAGAGGCAGGTTTCTTTGCAGCTAAGGTACTGCAGAGAAGAGAGTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

133

Amino Acids

15.54

Weight (kDa)

9.6

Isoelectric Point (pI)

51.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF761 PF05553 96 - 127 9.2e-10 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 293
Acc36I ACCTGC 1 cut(s) 346
AccII CGCG 2 cut(s) 139, 216
AciI CCGC 4 cut(s) 47, 97, 139, 216
AclWI GGATC 2 cut(s) 103, 270
AcsI RAATTY 1 cut(s) 325
AfaI GTAC 1 cut(s) 377
AfiI CCNNNNNNNGG 1 cut(s) 43
AgeI ACCGGT 1 cut(s) 162
AgsI TTSAA 4 cut(s) 82, 157, 188, 233
AluBI AGCT 7 cut(s) 78, 104, 131, 153, 180, 340, 370
AluI AGCT 7 cut(s) 78, 104, 131, 153, 180, 340, 370
AlwI GGATC 2 cut(s) 103, 270
Ama87I CYCGRG 1 cut(s) 126
ApeKI GCWGC 3 cut(s) 180, 337, 367
ApoI RAATTY 1 cut(s) 325
AsiGI ACCGGT 1 cut(s) 162
AspLEI GCGC 2 cut(s) 122, 216
AspS9I GGNCC 1 cut(s) 243
AsuHPI GGTGA 1 cut(s) 61
AvaI CYCGRG 1 cut(s) 126
AvaII GGWCC 1 cut(s) 243
BauI CACGAG 1 cut(s) 238
BbsI GAAGAC 1 cut(s) 195
BbvI GCAGC 3 cut(s) 167, 349, 379
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 379
BfuAI ACCTGC 1 cut(s) 346
BisI GCNGC 4 cut(s) 140, 181, 338, 368
BlsI GCNGC 4 cut(s) 141, 182, 339, 369
Bme18I GGWCC 1 cut(s) 243
BmeT110I CYCGRG 1 cut(s) 126
BmgT120I GGNCC 1 cut(s) 243
BmiI GGNNCC 1 cut(s) 245
BpiI GAAGAC 1 cut(s) 195
Bpu10I CCTNAGC 1 cut(s) 371
BpuEI CTTGAG 1 cut(s) 23
BsaBI GATNNNNATC 1 cut(s) 297
BsaJI CCNNGG 2 cut(s) 112, 257
BsaWI WCCGGW 1 cut(s) 162
Bsc4I CCNNNNNNNGG 1 cut(s) 43
Bse118I RCCGGY 2 cut(s) 162, 333
Bse8I GATNNNNATC 1 cut(s) 297
BseDI CCNNGG 2 cut(s) 112, 257
BseJI GATNNNNATC 1 cut(s) 297
BseLI CCNNNNNNNGG 1 cut(s) 43
BseMII CTCAG 1 cut(s) 88
BseXI GCAGC 3 cut(s) 167, 349, 379
Bsh1236I CGCG 2 cut(s) 139, 216
BshTI ACCGGT 1 cut(s) 162
BsiHKCI CYCGRG 1 cut(s) 126
BsiSI CCGG 2 cut(s) 163, 334
BslI CCNNNNNNNGG 1 cut(s) 43
BsoBI CYCGRG 1 cut(s) 126
Bsp143I GATC 4 cut(s) 108, 250, 262, 277
BspACI CCGC 4 cut(s) 47, 97, 139, 216
BspCNI CTCAG 1 cut(s) 87
BspFNI CGCG 2 cut(s) 139, 216
BspLI GGNNCC 1 cut(s) 245
BspMAI CTGCAG 1 cut(s) 383
BspMI ACCTGC 1 cut(s) 346
BspPI GGATC 2 cut(s) 103, 270
BsrFI RCCGGY 2 cut(s) 162, 333
BssAI RCCGGY 2 cut(s) 162, 333
BssECI CCNNGG 2 cut(s) 112, 257
BssMI GATC 4 cut(s) 108, 250, 262, 277
BssSI CACGAG 1 cut(s) 238
BssT1I CCWWGG 1 cut(s) 112
Bst2BI CACGAG 1 cut(s) 238
Bst6I CTCTTC 1 cut(s) 381
BstC8I GCNNGC 1 cut(s) 335
BstDEI CTNAG 2 cut(s) 74, 371
BstFNI CGCG 2 cut(s) 139, 216
BstHHI GCGC 2 cut(s) 122, 216
BstKTI GATC 4 cut(s) 111, 253, 265, 280
BstMBI GATC 4 cut(s) 108, 250, 262, 277
BstMWI GCNNNNNNNGC 1 cut(s) 128
BstSFI CTRYAG 1 cut(s) 379
BstUI CGCG 2 cut(s) 139, 216
BstV1I GCAGC 3 cut(s) 167, 349, 379
BstV2I GAAGAC 1 cut(s) 195
BveI ACCTGC 1 cut(s) 346
Cac8I GCNNGC 1 cut(s) 335
CfoI GCGC 2 cut(s) 122, 216
Cfr10I RCCGGY 2 cut(s) 162, 333
Cfr13I GGNCC 1 cut(s) 243
Csp6I GTAC 1 cut(s) 376
CspAI ACCGGT 1 cut(s) 162
CviAII CATG 2 cut(s) 203, 248
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 2 cut(s) 74, 371
DpnI GATC 4 cut(s) 110, 252, 264, 279
DpnII GATC 4 cut(s) 108, 250, 262, 277
DrdI GACNNNNNNGTC 1 cut(s) 293
DseDI GACNNNNNNGTC 1 cut(s) 293
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
Eco130I CCWWGG 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 243
Eco88I CYCGRG 1 cut(s) 126
EcoO109I RGGNCCY 1 cut(s) 243
EcoT14I CCWWGG 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 112
FaeI CATG 2 cut(s) 206, 251
FaiI YATR 3 cut(s) 204, 249, 320
FatI CATG 2 cut(s) 202, 247
Fnu4HI GCNGC 4 cut(s) 140, 181, 338, 368
Fsp4HI GCNGC 4 cut(s) 140, 181, 338, 368
FspBI CTAG 1 cut(s) 6
GlaI GCGC 2 cut(s) 121, 215
GluI GCNGC 4 cut(s) 140, 181, 338, 368
HapII CCGG 2 cut(s) 163, 334
HhaI GCGC 2 cut(s) 122, 216
Hin1II CATG 2 cut(s) 206, 251
Hin6I GCGC 2 cut(s) 120, 214
HinP1I GCGC 2 cut(s) 120, 214
HinfI GANTC 2 cut(s) 208, 269
HpaII CCGG 2 cut(s) 163, 334
HphI GGTGA 1 cut(s) 61
Hpy188I TCNGA 2 cut(s) 258, 274
Hpy188III TCNNGA 4 cut(s) 40, 126, 266, 281
HpyAV CCTTC 1 cut(s) 219
HpyCH4V TGCA 3 cut(s) 183, 367, 381
HpyF10VI GCNNNNNNNGC 1 cut(s) 128
HpyF3I CTNAG 2 cut(s) 74, 371
Hsp92II CATG 2 cut(s) 206, 251
HspAI GCGC 2 cut(s) 120, 214
KroI GCCGGC 1 cut(s) 333
KroNI GCCGGC 1 cut(s) 335
Kzo9I GATC 4 cut(s) 108, 250, 262, 277
LpnPI CCDG 6 cut(s) 77, 176, 251, 289, 341, 347
Lsp1109I GCAGC 3 cut(s) 167, 349, 379
MaeI CTAG 1 cut(s) 6
MaeIII GTNAC 1 cut(s) 18
MalI GATC 4 cut(s) 110, 252, 264, 279
MboI GATC 4 cut(s) 108, 250, 262, 277
MboII GAAGA 3 cut(s) 64, 200, 398
MluCI AATT 2 cut(s) 83, 325
MnlI CCTC 5 cut(s) 36, 47, 234, 252, 345
MroNI GCCGGC 1 cut(s) 333
MseI TTAA 1 cut(s) 86
MspA1I CMGCKG 2 cut(s) 180, 340
MspI CCGG 2 cut(s) 163, 334
MvnI CGCG 2 cut(s) 139, 216
MwoI GCNNNNNNNGC 1 cut(s) 128
NaeI GCCGGC 1 cut(s) 335
NdeII GATC 4 cut(s) 108, 250, 262, 277
NgoMIV GCCGGC 1 cut(s) 333
NlaIII CATG 2 cut(s) 206, 251
NlaIV GGNNCC 1 cut(s) 245
NmuCI GTSAC 1 cut(s) 18
PaeR7I CTCGAG 1 cut(s) 126
PdiI GCCGGC 1 cut(s) 335
PfeI GAWTC 2 cut(s) 208, 269
PinAI ACCGGT 1 cut(s) 162
PkrI GCNGC 4 cut(s) 141, 182, 339, 369
PpuMI RGGWCCY 1 cut(s) 243
Psp5II RGGWCCY 1 cut(s) 243
PspN4I GGNNCC 1 cut(s) 245
PspPI GGNCC 1 cut(s) 243
PspPPI RGGWCCY 1 cut(s) 243
PstI CTGCAG 1 cut(s) 383
PvuII CAGCTG 2 cut(s) 180, 340
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
SaqAI TTAA 1 cut(s) 86
SatI GCNGC 4 cut(s) 140, 181, 338, 368
Sau3AI GATC 4 cut(s) 108, 250, 262, 277
Sau96I GGNCC 1 cut(s) 243
SfcI CTRYAG 1 cut(s) 379
Sfr274I CTCGAG 1 cut(s) 126
SinI GGWCC 1 cut(s) 243
SlaI CTCGAG 1 cut(s) 126
SmlI CTYRAG 2 cut(s) 38, 126
SmoI CTYRAG 2 cut(s) 38, 126
Sse9I AATT 2 cut(s) 83, 325
SsiI CCGC 4 cut(s) 47, 97, 139, 216
SspMI CTAG 1 cut(s) 6
StyI CCWWGG 1 cut(s) 112
TaqI TCGA 2 cut(s) 107, 127
TasI AATT 2 cut(s) 83, 325
TauI GCSGC 1 cut(s) 142
TfiI GAWTC 2 cut(s) 208, 269
Tru1I TTAA 1 cut(s) 86
Tru9I TTAA 1 cut(s) 86
TseFI GTSAC 1 cut(s) 18
TseI GCWGC 3 cut(s) 180, 337, 367
Tsp45I GTSAC 1 cut(s) 18
VpaK11BI GGWCC 1 cut(s) 243
XapI RAATTY 1 cut(s) 325
XhoI CTCGAG 1 cut(s) 126
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.