pycom15g09840

Cotton fibre expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006388
Physical Location & Seq
Forward (+)
6402931 .. 6403344
414 bp
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UTR
Exon/CDS
Intron
pycom15g09840.1

Sequence Viewer

Length: 414 bp
ATGGCTAGGGAAACCTTGACACTCGTAAGCCGCAGCCTCAAGAGGGCGGTGAACAAAATCAACCTCATTCTACTCAGCTTCAAATTAAACCGATTGCGCTTGGCTTCGATCCTCGGTTGCTGCTCTTCTCGAGCTTCTACGAGGCATTGTTTGAGCTTCAATGACCGGTTGGGGCTGTACAGCTGCATTGAAGATGAAAATACTAGTACTGATGGGAATCAACGCAGTTTGAGAAGGGTTCAAAGCACAAGGGCCCGTGATCTTCCGAAGGATCAGTCTTTGGATGATCACGATGGTGATGATGATGACGTCGATCGCAGGGCTGATGTTTTCATAGCGAATTTTCACCGGCAGCTGATGTTTGAGAGACAAGTTTCTTTGCAGCTAAGGTACTGCAGACGGGATAATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

138

Amino Acids

15.94

Weight (kDa)

9.22

Isoelectric Point (pI)

57.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF761 PF05553 101 - 133 2.3e-10 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 312
AciI CCGC 2 cut(s) 31, 47
AclWI GGATC 2 cut(s) 103, 279
AcsI RAATTY 1 cut(s) 340
AcyI GRCGYC 1 cut(s) 309
AfaI GTAC 3 cut(s) 179, 208, 392
AfiI CCNNNNNNNGG 1 cut(s) 43
AgeI ACCGGT 1 cut(s) 165
AgsI TTSAA 4 cut(s) 82, 160, 191, 242
AhlI ACTAGT 1 cut(s) 203
AleI CACNNNNGTG 1 cut(s) 294
AluBI AGCT 6 cut(s) 78, 134, 156, 183, 355, 385
AluI AGCT 6 cut(s) 78, 134, 156, 183, 355, 385
Alw26I GTCTC 1 cut(s) 361
AlwI GGATC 2 cut(s) 103, 279
Ama87I CYCGRG 1 cut(s) 129
AoxI GGCC 1 cut(s) 252
ApaI GGGCCC 1 cut(s) 256
ApeKI GCWGC 5 cut(s) 33, 120, 183, 352, 382
ApoI RAATTY 1 cut(s) 340
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
AsiGI ACCGGT 1 cut(s) 165
AspLEI GCGC 1 cut(s) 99
AspS9I GGNCC 2 cut(s) 252, 253
AsuHPI GGTGA 3 cut(s) 61, 308, 338
AvaI CYCGRG 1 cut(s) 129
BaeGI GKGCMC 1 cut(s) 256
BanII GRGCYC 1 cut(s) 256
BbvI GCAGC 5 cut(s) 45, 107, 170, 364, 394
BccI CCATC 2 cut(s) 206, 287
BclI TGATCA 1 cut(s) 286
BcoDI GTCTC 1 cut(s) 361
BcuI ACTAGT 1 cut(s) 203
BfaI CTAG 2 cut(s) 6, 204
BfmI CTRYAG 1 cut(s) 394
BisI GCNGC 6 cut(s) 31, 34, 121, 184, 353, 383
BlsI GCNGC 6 cut(s) 32, 35, 122, 185, 354, 384
BmcAI AGTACT 1 cut(s) 208
BmeT110I CYCGRG 1 cut(s) 129
BmgT120I GGNCC 2 cut(s) 252, 253
BmiI GGNNCC 1 cut(s) 254
Bpu10I CCTNAGC 1 cut(s) 386
BpuEI CTTGAG 1 cut(s) 23
BsaBI GATNNNNATC 1 cut(s) 216
BsaHI GRCGYC 1 cut(s) 309
BsaJI CCNNGG 1 cut(s) 112
BsaWI WCCGGW 1 cut(s) 165
Bsc4I CCNNNNNNNGG 1 cut(s) 43
Bse118I RCCGGY 2 cut(s) 165, 348
Bse8I GATNNNNATC 1 cut(s) 216
BseDI CCNNGG 1 cut(s) 112
BseGI GGATG 1 cut(s) 289
BseJI GATNNNNATC 1 cut(s) 216
BseLI CCNNNNNNNGG 1 cut(s) 43
BseMII CTCAG 1 cut(s) 88
BseSI GKGCMC 1 cut(s) 256
BseXI GCAGC 5 cut(s) 45, 107, 170, 364, 394
Bsh1285I CGRYCG 1 cut(s) 316
BshFI GGCC 1 cut(s) 254
BshTI ACCGGT 1 cut(s) 165
BsiEI CGRYCG 1 cut(s) 316
BsiHKCI CYCGRG 1 cut(s) 129
BsiSI CCGG 2 cut(s) 166, 349
BslI CCNNNNNNNGG 1 cut(s) 43
BsmAI GTCTC 1 cut(s) 361
BsnI GGCC 1 cut(s) 254
BsoBI CYCGRG 1 cut(s) 129
Bsp120I GGGCCC 1 cut(s) 252
Bsp1286I GDGCHC 1 cut(s) 256
Bsp1407I TGTACA 1 cut(s) 177
Bsp143I GATC 5 cut(s) 108, 259, 271, 286, 313
BspACI CCGC 2 cut(s) 31, 47
BspANI GGCC 1 cut(s) 254
BspCNI CTCAG 1 cut(s) 87
BspLI GGNNCC 1 cut(s) 254
BspMAI CTGCAG 1 cut(s) 398
BspPI GGATC 2 cut(s) 103, 279
BspQI GCTCTTC 1 cut(s) 130
BsrFI RCCGGY 2 cut(s) 165, 348
BsrGI TGTACA 1 cut(s) 177
BssAI RCCGGY 2 cut(s) 165, 348
BssECI CCNNGG 1 cut(s) 112
BssMI GATC 5 cut(s) 108, 259, 271, 286, 313
BssNI GRCGYC 1 cut(s) 309
Bst6I CTCTTC 1 cut(s) 130
BstACI GRCGYC 1 cut(s) 309
BstAUI TGTACA 1 cut(s) 177
BstDEI CTNAG 2 cut(s) 74, 386
BstF5I GGATG 1 cut(s) 289
BstHHI GCGC 1 cut(s) 99
BstKTI GATC 5 cut(s) 111, 262, 274, 289, 316
BstMAI GTCTC 1 cut(s) 361
BstMBI GATC 5 cut(s) 108, 259, 271, 286, 313
BstMCI CGRYCG 1 cut(s) 316
BstSFI CTRYAG 1 cut(s) 394
BstSLI GKGCMC 1 cut(s) 256
BstV1I GCAGC 5 cut(s) 45, 107, 170, 364, 394
BsuRI GGCC 1 cut(s) 254
BtsCI GGATG 1 cut(s) 289
CfoI GCGC 1 cut(s) 99
Cfr10I RCCGGY 2 cut(s) 165, 348
Cfr13I GGNCC 2 cut(s) 252, 253
Csp6I GTAC 3 cut(s) 178, 207, 391
CspAI ACCGGT 1 cut(s) 165
CviQI GTAC 3 cut(s) 178, 207, 391
DdeI CTNAG 2 cut(s) 74, 386
DpnI GATC 5 cut(s) 110, 261, 273, 288, 315
DpnII GATC 5 cut(s) 108, 259, 271, 286, 313
Eam1104I CTCTTC 1 cut(s) 130
EarI CTCTTC 1 cut(s) 130
Eco24I GRGCYC 1 cut(s) 256
Eco88I CYCGRG 1 cut(s) 129
EcoO109I RGGNCCY 1 cut(s) 252
EcoT38I GRGCYC 1 cut(s) 256
FaiI YATR 1 cut(s) 335
FbaI TGATCA 1 cut(s) 286
Fnu4HI GCNGC 6 cut(s) 31, 34, 121, 184, 353, 383
FokI GGATG 1 cut(s) 296
FriOI GRGCYC 1 cut(s) 256
Fsp4HI GCNGC 6 cut(s) 31, 34, 121, 184, 353, 383
FspBI CTAG 2 cut(s) 6, 204
GlaI GCGC 1 cut(s) 98
GluI GCNGC 6 cut(s) 31, 34, 121, 184, 353, 383
HaeIII GGCC 1 cut(s) 254
HapII CCGG 2 cut(s) 166, 349
HhaI GCGC 1 cut(s) 99
Hin1I GRCGYC 1 cut(s) 309
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HinfI GANTC 1 cut(s) 217
HpaII CCGG 2 cut(s) 166, 349
HphI GGTGA 3 cut(s) 61, 308, 338
Hpy166II GTNNAC 1 cut(s) 52
Hpy188I TCNGA 1 cut(s) 267
Hpy188III TCNNGA 3 cut(s) 40, 129, 290
Hpy8I GTNNAC 1 cut(s) 52
Hpy99I CGWCG 1 cut(s) 314
HpyAV CCTTC 2 cut(s) 228, 262
HpyCH4IV ACGT 1 cut(s) 309
HpyCH4V TGCA 3 cut(s) 186, 382, 396
HpyF3I CTNAG 2 cut(s) 74, 386
HpySE526I ACGT 1 cut(s) 309
Hsp92I GRCGYC 1 cut(s) 309
HspAI GCGC 1 cut(s) 97
Ksp22I TGATCA 1 cut(s) 286
Kzo9I GATC 5 cut(s) 108, 259, 271, 286, 313
LguI GCTCTTC 1 cut(s) 130
LpnPI CCDG 3 cut(s) 179, 304, 362
Lsp1109I GCAGC 5 cut(s) 45, 107, 170, 364, 394
MaeI CTAG 2 cut(s) 6, 204
MaeII ACGT 1 cut(s) 309
MalI GATC 5 cut(s) 110, 261, 273, 288, 315
MboI GATC 5 cut(s) 108, 259, 271, 286, 313
MboII GAAGA 3 cut(s) 117, 203, 254
MhlI GDGCHC 1 cut(s) 256
MluCI AATT 3 cut(s) 83, 340, 406
MnlI CCTC 5 cut(s) 36, 47, 74, 122, 135
MseI TTAA 1 cut(s) 86
MslI CAYNNNNRTG 1 cut(s) 294
MspA1I CMGCKG 2 cut(s) 183, 355
MspI CCGG 2 cut(s) 166, 349
NdeII GATC 5 cut(s) 108, 259, 271, 286, 313
NlaIV GGNNCC 1 cut(s) 254
OliI CACNNNNGTG 1 cut(s) 294
PaeR7I CTCGAG 1 cut(s) 129
PciSI GCTCTTC 1 cut(s) 130
PfeI GAWTC 1 cut(s) 217
PinAI ACCGGT 1 cut(s) 165
PkrI GCNGC 6 cut(s) 32, 35, 122, 185, 354, 384
Ple19I CGATCG 1 cut(s) 316
PspN4I GGNNCC 1 cut(s) 254
PspOMI GGGCCC 1 cut(s) 252
PspPI GGNCC 2 cut(s) 252, 253
PstI CTGCAG 1 cut(s) 398
PvuI CGATCG 1 cut(s) 316
PvuII CAGCTG 2 cut(s) 183, 355
RsaI GTAC 3 cut(s) 179, 208, 392
RsaNI GTAC 3 cut(s) 178, 207, 391
RseI CAYNNNNRTG 1 cut(s) 294
SapI GCTCTTC 1 cut(s) 130
SaqAI TTAA 1 cut(s) 86
SatI GCNGC 6 cut(s) 31, 34, 121, 184, 353, 383
Sau3AI GATC 5 cut(s) 108, 259, 271, 286, 313
Sau96I GGNCC 2 cut(s) 252, 253
ScaI AGTACT 1 cut(s) 208
SduI GDGCHC 1 cut(s) 256
SfcI CTRYAG 1 cut(s) 394
Sfr274I CTCGAG 1 cut(s) 129
SlaI CTCGAG 1 cut(s) 129
SmiMI CAYNNNNRTG 1 cut(s) 294
SmlI CTYRAG 2 cut(s) 38, 129
SmoI CTYRAG 2 cut(s) 38, 129
SpeI ACTAGT 1 cut(s) 203
Sse9I AATT 3 cut(s) 83, 340, 406
SsiI CCGC 2 cut(s) 31, 47
SspMI CTAG 2 cut(s) 6, 204
TaiI ACGT 1 cut(s) 312
TaqI TCGA 3 cut(s) 107, 130, 312
TasI AATT 3 cut(s) 83, 340, 406
TatI WGTACW 2 cut(s) 177, 206
TauI GCSGC 1 cut(s) 33
TfiI GAWTC 1 cut(s) 217
Tru1I TTAA 1 cut(s) 86
Tru9I TTAA 1 cut(s) 86
TseI GCWGC 5 cut(s) 33, 120, 183, 352, 382
TspDTI ATGAA 2 cut(s) 210, 322
XapI RAATTY 1 cut(s) 340
XhoI CTCGAG 1 cut(s) 129
XspI CTAG 2 cut(s) 6, 204
ZraI GACGTC 1 cut(s) 310
ZrmI AGTACT 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.