MD08G1183000.v1.1

signal peptidase complex subunit 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009961
Physical Location & Seq
Reverse (-)
22686776 .. 22689724
2949 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1183000.v1.1.491

Sequence Viewer

Length: 333 bp
ATGGCAAACGATGCCGTTTTCAGATCTTCACTTGTTTGGTTAGCTGTAATTATGGCGGTTGTTGGAATATATACTCAGTCGTTCAAGAAGATGATATTAACGTACGTGGTTGGTGTGCTGGGTATTGCTGGGCTGCTTTTGCCTGATTGGGGTTACTTTGACCGCGACTTCTCGCGGTGGACCTCTCCGGTTTCCTTCGAGGAGAGAGCCTATGAAATTGCTCAAAGATCTGGATTAATAACAAGATTTAGAAAGTATCCCATGAGATTGGTTGCTTATGCCACAGTTTATGGGTTTGCTCTGTACAAGTGGTGGATGTTCATATCAACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPC12

Protein Analysis

111

Amino Acids

12.79

Weight (kDa)

9.69

Isoelectric Point (pI)

42.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPC12 PF06645 7 - 63 1.5e-08 Microsomal signal peptidase 12 kDa subunit (SPC12)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 165, 175
AciI CCGC 3 cut(s) 56, 163, 175
AfaI GTAC 2 cut(s) 104, 305
AfiI CCNNNNNNNGG 1 cut(s) 149
AgsI TTSAA 1 cut(s) 85
AluBI AGCT 1 cut(s) 44
AluI AGCT 1 cut(s) 44
ApeKI GCWGC 1 cut(s) 133
AseI ATTAAT 1 cut(s) 236
AspS9I GGNCC 1 cut(s) 180
AvaII GGWCC 1 cut(s) 180
BbvI GCAGC 1 cut(s) 120
BciVI GTATCC 1 cut(s) 267
BfuI GTATCC 1 cut(s) 267
BglII AGATCT 2 cut(s) 23, 227
BisI GCNGC 1 cut(s) 134
BlsI GCNGC 1 cut(s) 135
Bme18I GGWCC 1 cut(s) 180
BmgT120I GGNCC 1 cut(s) 180
BsaAI YACGTR 1 cut(s) 106
BsaWI WCCGGW 1 cut(s) 187
Bsc4I CCNNNNNNNGG 1 cut(s) 149
BseGI GGATG 1 cut(s) 321
BseLI CCNNNNNNNGG 1 cut(s) 149
BseMII CTCAG 1 cut(s) 89
BseRI GAGGAG 1 cut(s) 215
BseXI GCAGC 1 cut(s) 120
BseYI CCCAGC 2 cut(s) 118, 128
Bsh1236I CGCG 2 cut(s) 165, 175
BsiSI CCGG 1 cut(s) 188
BsiWI CGTACG 1 cut(s) 102
BslI CCNNNNNNNGG 1 cut(s) 149
Bsp1407I TGTACA 1 cut(s) 303
Bsp143I GATC 2 cut(s) 23, 227
BspACI CCGC 3 cut(s) 56, 163, 175
BspCNI CTCAG 1 cut(s) 88
BspFNI CGCG 2 cut(s) 165, 175
BsrGI TGTACA 1 cut(s) 303
BssMI GATC 2 cut(s) 23, 227
Bst4CI ACNGT 1 cut(s) 286
BstAPI GCANNNNNTGC 1 cut(s) 11
BstAUI TGTACA 1 cut(s) 303
BstBAI YACGTR 1 cut(s) 106
BstDEI CTNAG 1 cut(s) 75
BstF5I GGATG 1 cut(s) 321
BstFNI CGCG 2 cut(s) 165, 175
BstKTI GATC 2 cut(s) 26, 230
BstMBI GATC 2 cut(s) 23, 227
BstMWI GCNNNNNNNGC 2 cut(s) 11, 139
BstUI CGCG 2 cut(s) 165, 175
BstV1I GCAGC 1 cut(s) 120
BstX2I RGATCY 2 cut(s) 23, 227
BstXI CCANNNNNNTGG 1 cut(s) 268
BstYI RGATCY 2 cut(s) 23, 227
BsuI GTATCC 1 cut(s) 267
BtsCI GGATG 1 cut(s) 321
Cfr13I GGNCC 1 cut(s) 180
Csp6I GTAC 2 cut(s) 103, 304
CviAII CATG 1 cut(s) 262
CviJI RGCY 3 cut(s) 44, 133, 209
CviKI_1 RGCY 3 cut(s) 44, 133, 209
CviQI GTAC 2 cut(s) 103, 304
DdeI CTNAG 1 cut(s) 75
DpnI GATC 2 cut(s) 25, 229
DpnII GATC 2 cut(s) 23, 227
Eco47I GGWCC 1 cut(s) 180
FaeI CATG 1 cut(s) 265
FaiI YATR 8 cut(s) 53, 70, 72, 213, 263, 279, 291, 323
FatI CATG 1 cut(s) 261
Fnu4HI GCNGC 1 cut(s) 134
FokI GGATG 1 cut(s) 328
Fsp4HI GCNGC 1 cut(s) 134
GluI GCNGC 1 cut(s) 134
GsaI CCCAGC 2 cut(s) 122, 132
HapII CCGG 1 cut(s) 188
Hin1II CATG 1 cut(s) 265
HpaII CCGG 1 cut(s) 188
Hpy166II GTNNAC 1 cut(s) 180
Hpy188I TCNGA 1 cut(s) 23
Hpy188III TCNNGA 2 cut(s) 85, 231
Hpy8I GTNNAC 1 cut(s) 180
HpyAV CCTTC 1 cut(s) 205
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4IV ACGT 2 cut(s) 101, 105
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 139
HpyF3I CTNAG 1 cut(s) 75
HpySE526I ACGT 2 cut(s) 101, 105
Hsp92II CATG 1 cut(s) 265
Kzo9I GATC 2 cut(s) 23, 227
LpnPI CCDG 5 cut(s) 104, 114, 156, 201, 216
Lsp1109I GCAGC 1 cut(s) 120
MaeII ACGT 2 cut(s) 101, 105
MaeIII GTNAC 1 cut(s) 152
MalI GATC 2 cut(s) 25, 229
MboI GATC 2 cut(s) 23, 227
MboII GAAGA 2 cut(s) 18, 100
MflI RGATCY 2 cut(s) 23, 227
MluCI AATT 2 cut(s) 48, 216
MmeI TCCRAC 1 cut(s) 43
MnlI CCTC 2 cut(s) 193, 193
MseI TTAA 2 cut(s) 98, 236
MspI CCGG 1 cut(s) 188
MvnI CGCG 2 cut(s) 165, 175
MwoI GCNNNNNNNGC 2 cut(s) 11, 139
NdeII GATC 2 cut(s) 23, 227
NlaIII CATG 1 cut(s) 265
Pfl23II CGTACG 1 cut(s) 102
PkrI GCNGC 1 cut(s) 135
Ppu21I YACGTR 1 cut(s) 106
PshBI ATTAAT 1 cut(s) 236
PspFI CCCAGC 2 cut(s) 118, 128
PspLI CGTACG 1 cut(s) 102
PspPI GGNCC 1 cut(s) 180
PsuI RGATCY 2 cut(s) 23, 227
RsaI GTAC 2 cut(s) 104, 305
RsaNI GTAC 2 cut(s) 103, 304
SaqAI TTAA 2 cut(s) 98, 236
SatI GCNGC 1 cut(s) 134
Sau3AI GATC 2 cut(s) 23, 227
Sau96I GGNCC 1 cut(s) 180
SetI ASST 5 cut(s) 46, 104, 108, 185, 332
SinI GGWCC 1 cut(s) 180
Sse9I AATT 2 cut(s) 48, 216
SsiI CCGC 3 cut(s) 56, 163, 175
TaaI ACNGT 1 cut(s) 286
TaiI ACGT 2 cut(s) 104, 108
TaqI TCGA 1 cut(s) 198
TasI AATT 2 cut(s) 48, 216
TatI WGTACW 1 cut(s) 303
Tru1I TTAA 2 cut(s) 98, 236
Tru9I TTAA 2 cut(s) 98, 236
TseI GCWGC 1 cut(s) 133
TspDTI ATGAA 2 cut(s) 228, 310
VpaK11BI GGWCC 1 cut(s) 180
VspI ATTAAT 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.