Rh7CG496200

signal peptidase complex subunit 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0009961
Physical Location & Seq
Forward (+)
66805714 .. 66810756
5043 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG496200.1

Sequence Viewer

Length: 471 bp
ATGGCCAACCACGCCGTCTTCAGGTCCTCTCTGGTTTGGTTAGCGCTAGTTCTCGTGGTTGTTGGGATTTGCACTCAGTCTTTGAAGAAGATCATGGTGACTTACCTGGTTGGTGTGATCGGAATCGGGGGTGTGCTTTTACCTGATTGGGACTACTTTGACCGGGATTTTTCCCGGTGGAGTTCTCCGGTTACGGTTGAGGAAAGGGCCTCTGCGATTGCAACTGCTGAAAGATCTGGATTAGCAAGATTCAAAAGGCATCCTATGAGATTGGCTGCTTATGCCACAATTTATGGGTTTGCTCTGTACAAATGGTGGAGTCCCTCAATATCACAACACTTGCTTGCTGTAACTCTCACTCTCTCTCTCTCGATCGGCCGGCAACAATTGCAGTCCCCGTCTCTCGAGAATCGCAGGTTCTTAAACAGAAGGGAAGCAACACTCTGTACTGCTGGCTACTGCCCTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPC12

Protein Analysis

156

Amino Acids

17.51

Weight (kDa)

9.87

Isoelectric Point (pI)

60.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPC12 PF06645 8 - 63 6.1e-07 Microsomal signal peptidase 12 kDa subunit (SPC12)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 405
AcoI YGGCCR 2 cut(s) 3, 376
AcuI CTGAAG 1 cut(s) 4
AfaI GTAC 2 cut(s) 308, 448
AfeI AGCGCT 1 cut(s) 45
AfiI CCNNNNNNNGG 1 cut(s) 21
AgsI TTSAA 2 cut(s) 85, 253
AjnI CCWGG 1 cut(s) 105
Alw26I GTCTC 1 cut(s) 405
Ama87I CYCGRG 1 cut(s) 404
Aor51HI AGCGCT 1 cut(s) 45
AoxI GGCC 3 cut(s) 3, 207, 376
ApeKI GCWGC 1 cut(s) 275
AspLEI GCGC 1 cut(s) 46
AspS9I GGNCC 2 cut(s) 24, 207
AsuC2I CCSGG 2 cut(s) 164, 175
AsuHPI GGTGA 1 cut(s) 109
AvaI CYCGRG 1 cut(s) 404
AvaII GGWCC 1 cut(s) 24
BalI TGGCCA 1 cut(s) 5
BauI CACGAG 1 cut(s) 53
BbsI GAAGAC 1 cut(s) 10
BbvI GCAGC 1 cut(s) 262
BciT130I CCWGG 1 cut(s) 107
BcnI CCSGG 2 cut(s) 164, 175
BcoDI GTCTC 1 cut(s) 405
BfaI CTAG 1 cut(s) 47
BfmI CTRYAG 1 cut(s) 467
BfoI RGCGCY 1 cut(s) 47
BfuAI ACCTGC 1 cut(s) 405
BglII AGATCT 1 cut(s) 233
BisI GCNGC 1 cut(s) 276
BlsI GCNGC 1 cut(s) 277
Bme1390I CCNGG 3 cut(s) 107, 164, 175
Bme18I GGWCC 1 cut(s) 24
BmeT110I CYCGRG 1 cut(s) 404
BmgT120I GGNCC 2 cut(s) 24, 207
BmrFI CCNGG 3 cut(s) 107, 164, 175
BmsI GCATC 1 cut(s) 268
BpiI GAAGAC 1 cut(s) 10
BpuMI CCSGG 2 cut(s) 164, 175
BsaBI GATNNNNATC 1 cut(s) 122
BsaWI WCCGGW 1 cut(s) 187
Bsc4I CCNNNNNNNGG 1 cut(s) 21
Bse118I RCCGGY 1 cut(s) 378
Bse8I GATNNNNATC 1 cut(s) 122
BseBI CCWGG 1 cut(s) 107
BseGI GGATG 1 cut(s) 259
BseJI GATNNNNATC 1 cut(s) 122
BseLI CCNNNNNNNGG 1 cut(s) 21
BseMII CTCAG 1 cut(s) 89
BseX3I CGGCCG 1 cut(s) 376
BseXI GCAGC 1 cut(s) 262
Bsh1285I CGRYCG 2 cut(s) 375, 379
BshFI GGCC 3 cut(s) 5, 209, 378
BsiEI CGRYCG 2 cut(s) 375, 379
BsiHKCI CYCGRG 1 cut(s) 404
BsiSI CCGG 4 cut(s) 163, 175, 188, 379
BslFI GGGAC 3 cut(s) 164, 306, 379
BslI CCNNNNNNNGG 1 cut(s) 21
BsmAI GTCTC 1 cut(s) 405
BsmBI CGTCTC 1 cut(s) 405
BsmFI GGGAC 3 cut(s) 164, 306, 379
BsnI GGCC 3 cut(s) 5, 209, 378
BsoBI CYCGRG 1 cut(s) 404
Bsp1407I TGTACA 1 cut(s) 306
Bsp143I GATC 4 cut(s) 90, 117, 233, 372
BspANI GGCC 3 cut(s) 5, 209, 378
BspCNI CTCAG 1 cut(s) 88
BspMI ACCTGC 1 cut(s) 405
BsrFI RCCGGY 1 cut(s) 378
BsrGI TGTACA 1 cut(s) 306
BssAI RCCGGY 1 cut(s) 378
BssMI GATC 4 cut(s) 90, 117, 233, 372
BssSI CACGAG 1 cut(s) 53
Bst2BI CACGAG 1 cut(s) 53
Bst2UI CCWGG 1 cut(s) 107
Bst4CI ACNGT 1 cut(s) 196
BstAPI GCANNNNNTGC 1 cut(s) 388
BstAUI TGTACA 1 cut(s) 306
BstC8I GCNNGC 3 cut(s) 345, 380, 454
BstDEI CTNAG 1 cut(s) 75
BstF5I GGATG 1 cut(s) 259
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 1 cut(s) 46
BstKTI GATC 4 cut(s) 93, 120, 236, 375
BstMAI GTCTC 1 cut(s) 405
BstMBI GATC 4 cut(s) 90, 117, 233, 372
BstMCI CGRYCG 2 cut(s) 375, 379
BstMWI GCNNNNNNNGC 3 cut(s) 11, 281, 388
BstNI CCWGG 1 cut(s) 107
BstSCI CCNGG 3 cut(s) 105, 162, 173
BstSFI CTRYAG 1 cut(s) 467
BstV1I GCAGC 1 cut(s) 262
BstV2I GAAGAC 1 cut(s) 10
BstX2I RGATCY 1 cut(s) 233
BstYI RGATCY 1 cut(s) 233
BstZI CGGCCG 1 cut(s) 376
BsuRI GGCC 3 cut(s) 5, 209, 378
BtsCI GGATG 1 cut(s) 259
BveI ACCTGC 1 cut(s) 405
Cac8I GCNNGC 3 cut(s) 345, 380, 454
CfoI GCGC 1 cut(s) 46
Cfr10I RCCGGY 1 cut(s) 378
Cfr13I GGNCC 2 cut(s) 24, 207
CsiI ACCWGGT 1 cut(s) 105
Csp6I GTAC 2 cut(s) 307, 447
CviAII CATG 1 cut(s) 94
CviJI RGCY 5 cut(s) 5, 209, 275, 378, 456
CviKI_1 RGCY 5 cut(s) 5, 209, 275, 378, 456
CviQI GTAC 2 cut(s) 307, 447
DdeI CTNAG 1 cut(s) 75
DpnI GATC 4 cut(s) 92, 119, 235, 374
DpnII GATC 4 cut(s) 90, 117, 233, 372
EaeI YGGCCR 2 cut(s) 3, 376
EagI CGGCCG 1 cut(s) 376
EclXI CGGCCG 1 cut(s) 376
Eco47I GGWCC 1 cut(s) 24
Eco47III AGCGCT 1 cut(s) 45
Eco52I CGGCCG 1 cut(s) 376
Eco57I CTGAAG 1 cut(s) 4
Eco88I CYCGRG 1 cut(s) 404
EcoO109I RGGNCCY 2 cut(s) 24, 207
EcoRII CCWGG 1 cut(s) 105
Esp3I CGTCTC 1 cut(s) 405
FaeI CATG 1 cut(s) 97
FaiI YATR 4 cut(s) 95, 266, 282, 294
FaqI GGGAC 3 cut(s) 164, 306, 379
FatI CATG 1 cut(s) 93
Fnu4HI GCNGC 1 cut(s) 276
FokI GGATG 1 cut(s) 246
Fsp4HI GCNGC 1 cut(s) 276
FspBI CTAG 1 cut(s) 47
GlaI GCGC 1 cut(s) 45
GluI GCNGC 1 cut(s) 276
HaeII RGCGCY 1 cut(s) 47
HaeIII GGCC 3 cut(s) 5, 209, 378
HapII CCGG 4 cut(s) 163, 175, 188, 379
HhaI GCGC 1 cut(s) 46
Hin1II CATG 1 cut(s) 97
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HinfI GANTC 4 cut(s) 123, 249, 319, 409
HpaII CCGG 4 cut(s) 163, 175, 188, 379
HphI GGTGA 1 cut(s) 109
Hpy188I TCNGA 1 cut(s) 122
Hpy188III TCNNGA 4 cut(s) 237, 370, 404, 406
HpyAV CCTTC 1 cut(s) 423
HpyCH4III ACNGT 1 cut(s) 196
HpyCH4V TGCA 3 cut(s) 72, 221, 391
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 281, 388
HpyF3I CTNAG 1 cut(s) 75
Hsp92II CATG 1 cut(s) 97
HspAI GCGC 1 cut(s) 44
KroI GCCGGC 1 cut(s) 378
KroNI GCCGGC 1 cut(s) 380
Kzo9I GATC 4 cut(s) 90, 117, 233, 372
Lsp1109I GCAGC 1 cut(s) 262
LweI GCATC 1 cut(s) 268
MabI ACCWGGT 1 cut(s) 105
MaeI CTAG 1 cut(s) 47
MaeIII GTNAC 3 cut(s) 97, 190, 349
MalI GATC 4 cut(s) 92, 119, 235, 374
MboI GATC 4 cut(s) 90, 117, 233, 372
MboII GAAGA 3 cut(s) 10, 97, 100
MfeI CAATTG 1 cut(s) 386
MflI RGATCY 1 cut(s) 233
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 288, 386
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 328
MnlI CCTC 4 cut(s) 37, 193, 220, 334
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 378
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 422
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 4 cut(s) 163, 175, 188, 379
MspR9I CCNGG 3 cut(s) 107, 164, 175
MunI CAATTG 1 cut(s) 386
MvaI CCWGG 1 cut(s) 107
MwoI GCNNNNNNNGC 3 cut(s) 11, 281, 388
NaeI GCCGGC 1 cut(s) 380
NciI CCSGG 2 cut(s) 164, 175
NdeII GATC 4 cut(s) 90, 117, 233, 372
NgoMIV GCCGGC 1 cut(s) 378
NlaIII CATG 1 cut(s) 97
NmuCI GTSAC 1 cut(s) 97
PaeR7I CTCGAG 1 cut(s) 404
PdiI GCCGGC 1 cut(s) 380
PfeI GAWTC 3 cut(s) 123, 249, 409
PkrI GCNGC 1 cut(s) 277
Ple19I CGATCG 1 cut(s) 375
PleI GAGTC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 327
PpuMI RGGWCCY 1 cut(s) 24
Psp5II RGGWCCY 1 cut(s) 24
Psp6I CCWGG 1 cut(s) 105
PspGI CCWGG 1 cut(s) 105
PspPI GGNCC 2 cut(s) 24, 207
PspPPI RGGWCCY 1 cut(s) 24
PsuI RGATCY 1 cut(s) 233
PvuI CGATCG 1 cut(s) 375
RsaI GTAC 2 cut(s) 308, 448
RsaNI GTAC 2 cut(s) 307, 447
SaqAI TTAA 1 cut(s) 422
SatI GCNGC 1 cut(s) 276
Sau3AI GATC 4 cut(s) 90, 117, 233, 372
Sau96I GGNCC 2 cut(s) 24, 207
SchI GAGTC 1 cut(s) 328
ScrFI CCNGG 3 cut(s) 107, 164, 175
SetI ASST 4 cut(s) 26, 108, 145, 419
SexAI ACCWGGT 1 cut(s) 105
SfaNI GCATC 1 cut(s) 268
SfcI CTRYAG 1 cut(s) 467
Sfr274I CTCGAG 1 cut(s) 404
SinI GGWCC 1 cut(s) 24
SlaI CTCGAG 1 cut(s) 404
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
Sse9I AATT 2 cut(s) 288, 386
SspMI CTAG 1 cut(s) 47
StyD4I CCNGG 3 cut(s) 105, 162, 173
TaaI ACNGT 1 cut(s) 196
TaqI TCGA 2 cut(s) 371, 405
TasI AATT 2 cut(s) 288, 386
TatI WGTACW 2 cut(s) 306, 446
TfiI GAWTC 3 cut(s) 123, 249, 409
Tru1I TTAA 1 cut(s) 422
Tru9I TTAA 1 cut(s) 422
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 1 cut(s) 275
Tsp45I GTSAC 1 cut(s) 97
VpaK11BI GGWCC 1 cut(s) 24
XhoI CTCGAG 1 cut(s) 404
XspI CTAG 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.