MD08G1206200.v1.1

XIAP-associated factor 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012125
Physical Location & Seq
Reverse (-)
26660878 .. 26671183
10306 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1206200.v1.1.491

Sequence Viewer

Length: 624 bp
ATGCAATCACTGGTGCTTCCTTGCACTAACTGTACTCTCCTAATGGATATTTGTTTTTATGTTTCCAGTGACAGAGCTATCCCCTCGTCAAATATTGATTTGCACTCTGCTCATTGCTTCCGCAATCTAGAAAAATGTAAAGTCTGTGGTGATATGGTTCCAAAAAAATATGCCAAGGAACATTACTCGAACACCCATGCTCCGGTACCCTGTTCACTTTGTAGTGAGACAATGGAACGTGAAATATTAGCCATCCACAAAGGTGAAAACTGCCCTCAAAGAATTGTCACGTGCGAGTTCTGTGAGTTCCCATTGCCTGCAATTGATCTTGCTGAGCATCAGGAAGTATGCGGGAATCGAACGGAACTTTGTCATCTTTGTAATAGATACATCAGACTGCGAGAACGCTATAACCATGAAATTAGGTGTAATGGTGTCTCAGATAATACTGCGGGTCCTTCCAGGGATGCTAGGCCAGCTGAAAGAAGACAAGGTCCTCCAAGAAGACAGGCAAACGAGTTTTCACGGAAGCGGCTTATATTCACCGTTGCACTAACAGGCATTGCTGTTCTATTAGGGTCTCTTTTTTTCCAGAGAAAGCCAGACGAGAGTCGTGTGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

208

Amino Acids

23.62

Weight (kDa)

8.36

Isoelectric Point (pI)

50.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012125)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09920
fragaria_vesca FvH4_5g34100 FvH4_5g34100
malus_domestica MD08G1206200.v1.1 MD15G1389500.v1.1
prunus_persica Prupe.1G538600_v2.0.a1 Prupe.1G538600_v2.0.a1
pyrus_communis pycom08g17820 pycom15g34910
rosa_chinensis RchiOBHm_Chr7g0233601
rosa_laevigata RLG00000001250
rosa_multiflora Rmu_sc0003712.1_g000011
rosa_roxburghii Rroxscaffold_3G00227120
rosa_rugosa Rorug07G0281400
rosa_samantha Rh7AG438000 Rh7BG410700 Rh7CG457600 Rh7DG426900
rosa_wichuraiana Rw7G036120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 205
AccB1I GGYRCC 1 cut(s) 205
AciI CCGC 4 cut(s) 121, 351, 452, 532
AcvI CACGTG 1 cut(s) 291
AfaI GTAC 2 cut(s) 34, 207
AfiI CCNNNNNNNGG 1 cut(s) 202
AjnI CCWGG 1 cut(s) 461
AleI CACNNNNGTG 1 cut(s) 261
AluBI AGCT 2 cut(s) 77, 479
AluI AGCT 2 cut(s) 77, 479
Alw26I GTCTC 3 cut(s) 221, 442, 585
AoxI GGCC 1 cut(s) 473
Asp718I GGTACC 1 cut(s) 205
AspS9I GGNCC 2 cut(s) 455, 494
AsuHPI GGTGA 3 cut(s) 161, 275, 535
AvaII GGWCC 2 cut(s) 455, 494
BanI GGYRCC 1 cut(s) 205
BbrPI CACGTG 1 cut(s) 291
BbsI GAAGAC 2 cut(s) 493, 511
BccI CCATC 1 cut(s) 260
BciT130I CCWGG 1 cut(s) 463
BcoDI GTCTC 3 cut(s) 221, 442, 585
BfaI CTAG 2 cut(s) 128, 471
BisI GCNGC 1 cut(s) 533
BlpI GCTNAGC 1 cut(s) 333
BlsI GCNGC 1 cut(s) 534
Bme1390I CCNGG 1 cut(s) 463
Bme18I GGWCC 2 cut(s) 455, 494
BmgT120I GGNCC 2 cut(s) 455, 494
BmiI GGNNCC 3 cut(s) 159, 207, 456
BmrFI CCNGG 1 cut(s) 463
BmsI GCATC 2 cut(s) 346, 457
BoxI GACNNNNGTC 1 cut(s) 609
BpiI GAAGAC 2 cut(s) 493, 511
Bpu1102I GCTNAGC 1 cut(s) 333
BsaAI YACGTR 1 cut(s) 291
BsaI GGTCTC 1 cut(s) 585
BsaJI CCNNGG 2 cut(s) 174, 462
BsaWI WCCGGW 1 cut(s) 202
BsaXI ACNNNNNCTCC 2 cut(s) 184, 214
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse1I ACTGG 2 cut(s) 15, 66
Bse3DI GCAATG 3 cut(s) 112, 311, 561
BseBI CCWGG 1 cut(s) 463
BseDI CCNNGG 2 cut(s) 174, 462
BseGI GGATG 2 cut(s) 252, 472
BseLI CCNNNNNNNGG 1 cut(s) 202
BseMI GCAATG 3 cut(s) 112, 311, 561
BseMII CTCAG 2 cut(s) 324, 453
BseNI ACTGG 2 cut(s) 15, 66
BshFI GGCC 1 cut(s) 475
BshNI GGYRCC 1 cut(s) 205
BsiSI CCGG 1 cut(s) 203
BslI CCNNNNNNNGG 1 cut(s) 202
BsmAI GTCTC 3 cut(s) 221, 442, 585
BsnI GGCC 1 cut(s) 475
Bso31I GGTCTC 1 cut(s) 585
Bsp143I GATC 1 cut(s) 325
Bsp1720I GCTNAGC 1 cut(s) 333
BspACI CCGC 4 cut(s) 121, 351, 452, 532
BspANI GGCC 1 cut(s) 475
BspCNI CTCAG 2 cut(s) 325, 452
BspLI GGNNCC 3 cut(s) 159, 207, 456
BspT107I GGYRCC 1 cut(s) 205
BspTNI GGTCTC 1 cut(s) 585
BsrDI GCAATG 3 cut(s) 112, 311, 561
BsrI ACTGG 2 cut(s) 15, 66
BssECI CCNNGG 2 cut(s) 174, 462
BssMI GATC 1 cut(s) 325
BssT1I CCWWGG 1 cut(s) 174
Bst2UI CCWGG 1 cut(s) 463
Bst4CI ACNGT 2 cut(s) 32, 547
BstBAI YACGTR 1 cut(s) 291
BstC8I GCNNGC 2 cut(s) 318, 477
BstDEI CTNAG 2 cut(s) 333, 439
BstF5I GGATG 2 cut(s) 252, 472
BstKTI GATC 1 cut(s) 328
BstMAI GTCTC 3 cut(s) 221, 442, 585
BstMBI GATC 1 cut(s) 325
BstMWI GCNNNNNNNGC 1 cut(s) 476
BstNI CCWGG 1 cut(s) 463
BstPAI GACNNNNGTC 1 cut(s) 609
BstSCI CCNGG 1 cut(s) 461
BstV2I GAAGAC 2 cut(s) 493, 511
BsuRI GGCC 1 cut(s) 475
BtsCI GGATG 2 cut(s) 252, 472
BtsIMutI CAGTG 2 cut(s) 8, 73
Cac8I GCNNGC 2 cut(s) 318, 477
Cfr13I GGNCC 2 cut(s) 455, 494
Csp6I GTAC 2 cut(s) 33, 206
CviAII CATG 2 cut(s) 197, 416
CviJI RGCY 6 cut(s) 77, 251, 475, 479, 535, 601
CviKI_1 RGCY 6 cut(s) 77, 251, 475, 479, 535, 601
CviQI GTAC 2 cut(s) 33, 206
DdeI CTNAG 2 cut(s) 333, 439
DpnI GATC 1 cut(s) 327
DpnII GATC 1 cut(s) 325
Eco130I CCWWGG 1 cut(s) 174
Eco31I GGTCTC 1 cut(s) 585
Eco47I GGWCC 2 cut(s) 455, 494
Eco72I CACGTG 1 cut(s) 291
EcoO109I RGGNCCY 2 cut(s) 455, 494
EcoRII CCWGG 1 cut(s) 461
EcoT14I CCWWGG 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 174
FaeI CATG 2 cut(s) 200, 419
FaiI YATR 8 cut(s) 60, 155, 171, 198, 349, 411, 417, 539
FatI CATG 2 cut(s) 196, 415
FauI CCCGC 2 cut(s) 344, 445
Fnu4HI GCNGC 1 cut(s) 533
FokI GGATG 2 cut(s) 239, 479
Fsp4HI GCNGC 1 cut(s) 533
FspBI CTAG 2 cut(s) 128, 471
GluI GCNGC 1 cut(s) 533
HaeIII GGCC 1 cut(s) 475
HapII CCGG 1 cut(s) 203
Hin1II CATG 2 cut(s) 200, 419
HinfI GANTC 2 cut(s) 355, 610
HpaII CCGG 1 cut(s) 203
HphI GGTGA 3 cut(s) 161, 275, 535
Hpy166II GTNNAC 1 cut(s) 215
Hpy188I TCNGA 2 cut(s) 395, 442
Hpy188III TCNNGA 3 cut(s) 128, 341, 592
Hpy8I GTNNAC 1 cut(s) 215
HpyAV CCTTC 1 cut(s) 468
HpyCH4III ACNGT 2 cut(s) 32, 547
HpyCH4IV ACGT 2 cut(s) 238, 290
HpyCH4V TGCA 6 cut(s) 4, 24, 103, 320, 551, 619
HpyF10VI GCNNNNNNNGC 1 cut(s) 476
HpyF3I CTNAG 2 cut(s) 333, 439
HpySE526I ACGT 2 cut(s) 238, 290
Hsp92II CATG 2 cut(s) 200, 419
KpnI GGTACC 1 cut(s) 209
Kzo9I GATC 1 cut(s) 325
LmnI GCTCC 1 cut(s) 205
LweI GCATC 2 cut(s) 346, 457
MaeI CTAG 2 cut(s) 128, 471
MaeII ACGT 2 cut(s) 238, 290
MaeIII GTNAC 2 cut(s) 68, 286
MalI GATC 1 cut(s) 327
MboI GATC 1 cut(s) 325
MboII GAAGA 2 cut(s) 498, 516
MfeI CAATTG 1 cut(s) 321
MluCI AATT 3 cut(s) 282, 321, 420
MlyI GAGTC 1 cut(s) 619
MnlI CCTC 3 cut(s) 94, 285, 507
MslI CAYNNNNRTG 1 cut(s) 261
MspA1I CMGCKG 1 cut(s) 479
MspI CCGG 1 cut(s) 203
MspR9I CCNGG 1 cut(s) 463
MunI CAATTG 1 cut(s) 321
MvaI CCWGG 1 cut(s) 463
MwoI GCNNNNNNNGC 1 cut(s) 476
NdeII GATC 1 cut(s) 325
NlaIII CATG 2 cut(s) 200, 419
NlaIV GGNNCC 3 cut(s) 159, 207, 456
NmuCI GTSAC 2 cut(s) 68, 286
OliI CACNNNNGTG 1 cut(s) 261
PfeI GAWTC 1 cut(s) 355
PflFI GACNNNGTC 1 cut(s) 492
PkrI GCNGC 1 cut(s) 534
PleI GAGTC 1 cut(s) 618
PmaCI CACGTG 1 cut(s) 291
PmlI CACGTG 1 cut(s) 291
PpsI GAGTC 1 cut(s) 618
Ppu21I YACGTR 1 cut(s) 291
PpuMI RGGWCCY 2 cut(s) 455, 494
PshAI GACNNNNGTC 1 cut(s) 609
Psp5II RGGWCCY 2 cut(s) 455, 494
Psp6I CCWGG 1 cut(s) 461
PspCI CACGTG 1 cut(s) 291
PspGI CCWGG 1 cut(s) 461
PspN4I GGNNCC 3 cut(s) 159, 207, 456
PspPI GGNCC 2 cut(s) 455, 494
PspPPI RGGWCCY 2 cut(s) 455, 494
PsyI GACNNNGTC 1 cut(s) 492
PvuII CAGCTG 1 cut(s) 479
RsaI GTAC 2 cut(s) 34, 207
RsaNI GTAC 2 cut(s) 33, 206
RseI CAYNNNNRTG 1 cut(s) 261
SatI GCNGC 1 cut(s) 533
Sau3AI GATC 1 cut(s) 325
Sau96I GGNCC 2 cut(s) 455, 494
SchI GAGTC 1 cut(s) 619
ScrFI CCNGG 1 cut(s) 463
SetI ASST 7 cut(s) 79, 241, 265, 293, 428, 481, 496
SfaNI GCATC 2 cut(s) 346, 457
SinI GGWCC 2 cut(s) 455, 494
SmiMI CAYNNNNRTG 1 cut(s) 261
Sse9I AATT 3 cut(s) 282, 321, 420
SsiI CCGC 4 cut(s) 121, 351, 452, 532
SspI AATATT 2 cut(s) 94, 246
SspMI CTAG 2 cut(s) 128, 471
StyD4I CCNGG 1 cut(s) 461
StyI CCWWGG 1 cut(s) 174
TaaI ACNGT 2 cut(s) 32, 547
TaiI ACGT 2 cut(s) 241, 293
TaqI TCGA 2 cut(s) 188, 358
TasI AATT 3 cut(s) 282, 321, 420
TatI WGTACW 1 cut(s) 32
TauI GCSGC 1 cut(s) 535
TfiI GAWTC 1 cut(s) 355
TscAI CASTG 2 cut(s) 15, 73
TseFI GTSAC 2 cut(s) 68, 286
Tsp45I GTSAC 2 cut(s) 68, 286
TspDTI ATGAA 1 cut(s) 432
TspGWI ACGGA 2 cut(s) 377, 541
TspRI CASTG 2 cut(s) 15, 73
Tth111I GACNNNGTC 1 cut(s) 492
VpaK11BI GGWCC 2 cut(s) 455, 494
XbaI TCTAGA 1 cut(s) 127
XspI CTAG 2 cut(s) 128, 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.