Rorug07G0281400

XIAP-associated factor 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0012125
Physical Location & Seq
Forward (+)
27283597 .. 27284568
972 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0281400.1

Sequence Viewer

Length: 873 bp
ATGGAAGCACAGAGCTTGAAAGAGGCCAATCCTAAATTGCAGAGCTTGGAAGTAGACCCTAAATCAAAGGGCTTGGAAGAAGAAGATACTAAATTACAGAGCTTGAAAGAAGAAGACCATAAATCACAGCGATTGAAAGAAGAGGAATGTAAGTCGCACAGCTCGGAAGAAGACCTGAAGTTAAACAGCTCGGAAGAAGACCCTAAATCGCAGAGCTTGAAAGAAGAAGACCCTAAATTACAGAGCTTGGAAGAGGAAGAGCCTAAATCACAGAGCTTGAAAGAAGGACGAGATAGCTTGGGAGACCCTAAATTCAATGAAAATGCATTGCAATTCCTGGATTCAATGGATGGGTATTTAACCCTAATGGATTCATTGTCTTGGACACTTCGGCAGGGATGGCTGCAACTAGCGAGTGCGCGCCACTCAATGGGTGGGTCGCGGGTGAGCAGTGCGGTGTTGGATATGAAGCCCCACTCAGCTGCTACTTTTTTGCAAGTCAGCAAACAATCCAATCCAGGAGTAGGGTTAGGGGAAATTCATCATTTTACATTGCTGAAATGGGCCTCATCGGATAATGGAAATGGAGCTCCACATAGAGATGTGAGTCCGCAGCTAAGACAAAGAAAGGACAAGGATGGAGATCCAGCCCCTGTTACAGCTGAAGATGACCATCATCAGCAACTTCAGAAGGAGCGGTTTAAATCACTTTCGGTGTTTGGCGCTTTGGTTTCCCCCAAGCTACGGGCGGCTCAGCTCTCATTCGAAACTGCACTGGAAGCACTTGTAGAGATTGCTAACATGCGATCGTTGATGCTTTCCAATTTTCATGAAGTCAGAAAAGAAATGGAATCAGCAAATGGCAAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

290

Amino Acids

32.44

Weight (kDa)

5.32

Isoelectric Point (pI)

62.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vma22_CCDC115 PF21730 107 - 257 3.1e-09 Vma22/CCDC115
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012125)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09920
fragaria_vesca FvH4_5g34100 FvH4_5g34100
malus_domestica MD08G1206200.v1.1 MD15G1389500.v1.1
prunus_persica Prupe.1G538600_v2.0.a1 Prupe.1G538600_v2.0.a1
pyrus_communis pycom08g17820 pycom15g34910
rosa_chinensis RchiOBHm_Chr7g0233601
rosa_laevigata RLG00000001250
rosa_multiflora Rmu_sc0003712.1_g000011
rosa_roxburghii Rroxscaffold_3G00227120
rosa_rugosa Rorug07G0281400
rosa_samantha Rh7AG438000 Rh7BG410700 Rh7CG457600 Rh7DG426900
rosa_wichuraiana Rw7G036120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 430
AccBSI CCGCTC 1 cut(s) 697
AccI GTMKAC 1 cut(s) 54
AccII CGCG 2 cut(s) 421, 442
AciI CCGC 5 cut(s) 442, 455, 611, 697, 749
AclWI GGATC 1 cut(s) 638
AcsI RAATTY 2 cut(s) 311, 537
AcuI CTGAAG 3 cut(s) 197, 671, 684
AfiI CCNNNNNNNGG 3 cut(s) 430, 524, 744
AgsI TTSAA 7 cut(s) 19, 106, 136, 220, 280, 316, 345
AjnI CCWGG 2 cut(s) 336, 517
Alw21I GWGCWC 1 cut(s) 592
Alw26I GTCTC 1 cut(s) 297
AlwI GGATC 1 cut(s) 638
AlwNI CAGNNNCTG 1 cut(s) 653
AoxI GGCC 2 cut(s) 24, 564
ApeKI GCWGC 3 cut(s) 403, 482, 613
ApoI RAATTY 2 cut(s) 311, 537
AspLEI GCGC 3 cut(s) 421, 423, 725
AspS9I GGNCC 1 cut(s) 564
AsuHPI GGTGA 1 cut(s) 457
AsuII TTCGAA 1 cut(s) 765
BanII GRGCYC 1 cut(s) 592
BbsI GAAGAC 4 cut(s) 120, 177, 204, 234
Bbv12I GWGCWC 1 cut(s) 592
BbvI GCAGC 3 cut(s) 390, 469, 625
BccI CCATC 4 cut(s) 344, 393, 632, 681
BciT130I CCWGG 2 cut(s) 338, 519
BcoDI GTCTC 1 cut(s) 297
BfaI CTAG 1 cut(s) 410
BfoI RGCGCY 1 cut(s) 726
BisI GCNGC 4 cut(s) 404, 483, 614, 750
BlpI GCTNAGC 1 cut(s) 753
BlsI GCNGC 4 cut(s) 405, 484, 615, 751
Bme1390I CCNGG 2 cut(s) 338, 519
BmgT120I GGNCC 1 cut(s) 564
BmrFI CCNGG 2 cut(s) 338, 519
BmsI GCATC 1 cut(s) 804
BpiI GAAGAC 4 cut(s) 120, 177, 204, 234
Bpu1102I GCTNAGC 1 cut(s) 753
Bpu14I TTCGAA 1 cut(s) 765
BsaBI GATNNNNATC 2 cut(s) 642, 672
BsaI GGTCTC 1 cut(s) 297
Bsc4I CCNNNNNNNGG 3 cut(s) 430, 524, 744
Bse1I ACTGG 1 cut(s) 780
Bse3DI GCAATG 2 cut(s) 326, 551
Bse8I GATNNNNATC 2 cut(s) 642, 672
BseBI CCWGG 2 cut(s) 338, 519
BseGI GGATG 3 cut(s) 355, 404, 643
BseJI GATNNNNATC 2 cut(s) 642, 672
BseLI CCNNNNNNNGG 3 cut(s) 430, 524, 744
BseMI GCAATG 2 cut(s) 326, 551
BseMII CTCAG 2 cut(s) 492, 767
BseNI ACTGG 1 cut(s) 780
BsePI GCGCGC 1 cut(s) 419
BseXI GCAGC 3 cut(s) 390, 469, 625
BsgI GTGCAG 1 cut(s) 756
Bsh1236I CGCG 2 cut(s) 421, 442
Bsh1285I CGRYCG 1 cut(s) 809
BshFI GGCC 2 cut(s) 26, 566
BsiEI CGRYCG 1 cut(s) 809
BsiHKAI GWGCWC 1 cut(s) 592
BslI CCNNNNNNNGG 3 cut(s) 430, 524, 744
BsmAI GTCTC 1 cut(s) 297
BsnI GGCC 2 cut(s) 26, 566
Bso31I GGTCTC 1 cut(s) 297
Bsp119I TTCGAA 1 cut(s) 765
Bsp1286I GDGCHC 1 cut(s) 592
Bsp143I GATC 2 cut(s) 643, 806
Bsp1720I GCTNAGC 1 cut(s) 753
BspACI CCGC 5 cut(s) 442, 455, 611, 697, 749
BspANI GGCC 2 cut(s) 26, 566
BspCNI CTCAG 2 cut(s) 491, 766
BspFNI CGCG 2 cut(s) 421, 442
BspHI TCATGA 1 cut(s) 829
BspPI GGATC 1 cut(s) 638
BspQI GCTCTTC 1 cut(s) 252
BspT104I TTCGAA 1 cut(s) 765
BspTNI GGTCTC 1 cut(s) 297
BsrBI CCGCTC 1 cut(s) 697
BsrDI GCAATG 2 cut(s) 326, 551
BsrI ACTGG 1 cut(s) 780
BssHII GCGCGC 1 cut(s) 419
BssMI GATC 2 cut(s) 643, 806
Bst2UI CCWGG 2 cut(s) 338, 519
Bst6I CTCTTC 3 cut(s) 135, 246, 252
BstBI TTCGAA 1 cut(s) 765
BstC8I GCNNGC 1 cut(s) 421
BstDEI CTNAG 3 cut(s) 478, 617, 753
BstF5I GGATG 3 cut(s) 355, 404, 643
BstFNI CGCG 2 cut(s) 421, 442
BstH2I RGCGCY 1 cut(s) 726
BstHHI GCGC 3 cut(s) 421, 423, 725
BstKTI GATC 2 cut(s) 646, 809
BstMAI GTCTC 1 cut(s) 297
BstMBI GATC 2 cut(s) 643, 806
BstMCI CGRYCG 1 cut(s) 809
BstMWI GCNNNNNNNGC 2 cut(s) 400, 779
BstNI CCWGG 2 cut(s) 338, 519
BstNSI RCATGY 1 cut(s) 805
BstSCI CCNGG 2 cut(s) 336, 517
BstUI CGCG 2 cut(s) 421, 442
BstV1I GCAGC 3 cut(s) 390, 469, 625
BstV2I GAAGAC 4 cut(s) 120, 177, 204, 234
BstX2I RGATCY 1 cut(s) 643
BstYI RGATCY 1 cut(s) 643
BsuRI GGCC 2 cut(s) 26, 566
BtsCI GGATG 3 cut(s) 355, 404, 643
BtsI GCAGTG 1 cut(s) 457
BtsIMutI CAGTG 2 cut(s) 457, 773
Cac8I GCNNGC 1 cut(s) 421
CaiI CAGNNNCTG 1 cut(s) 653
CciI TCATGA 1 cut(s) 829
CfoI GCGC 3 cut(s) 421, 423, 725
Cfr13I GGNCC 1 cut(s) 564
CviAII CATG 2 cut(s) 802, 830
DdeI CTNAG 3 cut(s) 478, 617, 753
DpnI GATC 2 cut(s) 645, 808
DpnII GATC 2 cut(s) 643, 806
DraI TTTAAA 1 cut(s) 703
Eam1104I CTCTTC 3 cut(s) 135, 246, 252
EarI CTCTTC 3 cut(s) 135, 246, 252
Ecl136II GAGCTC 1 cut(s) 590
Eco24I GRGCYC 1 cut(s) 592
Eco31I GGTCTC 1 cut(s) 297
Eco53kI GAGCTC 1 cut(s) 590
Eco57I CTGAAG 3 cut(s) 197, 671, 684
EcoICRI GAGCTC 1 cut(s) 590
EcoRII CCWGG 2 cut(s) 336, 517
EcoT22I ATGCAT 1 cut(s) 328
EcoT38I GRGCYC 1 cut(s) 592
FaeI CATG 2 cut(s) 805, 833
FaiI YATR 5 cut(s) 120, 467, 597, 803, 831
FatI CATG 2 cut(s) 801, 829
FauI CCCGC 1 cut(s) 435
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 4 cut(s) 404, 483, 614, 750
FokI GGATG 3 cut(s) 362, 411, 650
FriOI GRGCYC 1 cut(s) 592
Fsp4HI GCNGC 4 cut(s) 404, 483, 614, 750
FspBI CTAG 1 cut(s) 410
GlaI GCGC 3 cut(s) 420, 422, 724
GluI GCNGC 4 cut(s) 404, 483, 614, 750
HaeII RGCGCY 1 cut(s) 726
HaeIII GGCC 2 cut(s) 26, 566
HhaI GCGC 3 cut(s) 421, 423, 725
Hin1II CATG 2 cut(s) 805, 833
Hin6I GCGC 3 cut(s) 419, 421, 723
HinP1I GCGC 3 cut(s) 419, 421, 723
HinfI GANTC 4 cut(s) 341, 371, 607, 851
HphI GGTGA 1 cut(s) 457
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 5 cut(s) 166, 193, 574, 690, 839
Hpy188III TCNNGA 1 cut(s) 830
Hpy8I GTNNAC 1 cut(s) 55
HpyAV CCTTC 2 cut(s) 278, 685
HpyCH4V TGCA 6 cut(s) 40, 326, 331, 406, 496, 773
HpyF10VI GCNNNNNNNGC 2 cut(s) 400, 779
HpyF3I CTNAG 3 cut(s) 478, 617, 753
Hsp92II CATG 2 cut(s) 805, 833
HspAI GCGC 3 cut(s) 419, 421, 723
Kzo9I GATC 2 cut(s) 643, 806
LguI GCTCTTC 1 cut(s) 252
LmnI GCTCC 3 cut(s) 587, 595, 694
LpnPI CCDG 9 cut(s) 188, 323, 350, 380, 504, 531, 660, 666, 761
Lsp1109I GCAGC 3 cut(s) 390, 469, 625
LweI GCATC 1 cut(s) 804
MaeI CTAG 1 cut(s) 410
MaeIII GTNAC 1 cut(s) 655
MalI GATC 2 cut(s) 645, 808
MbiI CCGCTC 1 cut(s) 697
MboI GATC 2 cut(s) 643, 806
MflI RGATCY 1 cut(s) 643
MhlI GDGCHC 1 cut(s) 592
MluCI AATT 7 cut(s) 35, 92, 236, 311, 332, 537, 823
MlyI GAGTC 1 cut(s) 616
MmeI TCCRAC 1 cut(s) 441
MnlI CCTC 4 cut(s) 16, 136, 247, 577
Mph1103I ATGCAT 1 cut(s) 328
MseI TTAA 3 cut(s) 182, 359, 702
MslI CAYNNNNRTG 1 cut(s) 600
MspA1I CMGCKG 2 cut(s) 482, 662
MspR9I CCNGG 2 cut(s) 338, 519
MvaI CCWGG 2 cut(s) 338, 519
MvnI CGCG 2 cut(s) 421, 442
MwoI GCNNNNNNNGC 2 cut(s) 400, 779
NdeII GATC 2 cut(s) 643, 806
NlaIII CATG 2 cut(s) 805, 833
NsiI ATGCAT 1 cut(s) 328
NspI RCATGY 1 cut(s) 805
NspV TTCGAA 1 cut(s) 765
PagI TCATGA 1 cut(s) 829
PauI GCGCGC 1 cut(s) 419
PciSI GCTCTTC 1 cut(s) 252
PfeI GAWTC 3 cut(s) 341, 371, 851
PflMI CCANNNNNTGG 1 cut(s) 430
PfoI TCCNGGA 2 cut(s) 336, 517
PkrI GCNGC 4 cut(s) 405, 484, 615, 751
Ple19I CGATCG 1 cut(s) 809
PleI GAGTC 1 cut(s) 615
PpsI GAGTC 1 cut(s) 615
Psp124BI GAGCTC 1 cut(s) 592
Psp6I CCWGG 2 cut(s) 336, 517
PspGI CCWGG 2 cut(s) 336, 517
PspPI GGNCC 1 cut(s) 564
PstNI CAGNNNCTG 1 cut(s) 653
PsuI RGATCY 1 cut(s) 643
PteI GCGCGC 1 cut(s) 419
PvuI CGATCG 1 cut(s) 809
PvuII CAGCTG 2 cut(s) 482, 662
RseI CAYNNNNRTG 1 cut(s) 600
SacI GAGCTC 1 cut(s) 592
SapI GCTCTTC 1 cut(s) 252
SaqAI TTAA 3 cut(s) 182, 359, 702
SatI GCNGC 4 cut(s) 404, 483, 614, 750
Sau3AI GATC 2 cut(s) 643, 806
Sau96I GGNCC 1 cut(s) 564
SchI GAGTC 1 cut(s) 616
ScrFI CCNGG 2 cut(s) 338, 519
SduI GDGCHC 1 cut(s) 592
SfaNI GCATC 1 cut(s) 804
SfuI TTCGAA 1 cut(s) 765
SmiMI CAYNNNNRTG 1 cut(s) 600
Sse9I AATT 7 cut(s) 35, 92, 236, 311, 332, 537, 823
SsiI CCGC 5 cut(s) 442, 455, 611, 697, 749
SspMI CTAG 1 cut(s) 410
SstI GAGCTC 1 cut(s) 592
StyD4I CCNGG 2 cut(s) 336, 517
TaqI TCGA 1 cut(s) 765
TasI AATT 7 cut(s) 35, 92, 236, 311, 332, 537, 823
TauI GCSGC 1 cut(s) 752
TfiI GAWTC 3 cut(s) 341, 371, 851
Tru1I TTAA 3 cut(s) 182, 359, 702
Tru9I TTAA 3 cut(s) 182, 359, 702
TscAI CASTG 2 cut(s) 457, 780
TseI GCWGC 3 cut(s) 403, 482, 613
TspDTI ATGAA 6 cut(s) 333, 363, 482, 530, 818, 846
TspRI CASTG 2 cut(s) 457, 780
Van91I CCANNNNNTGG 1 cut(s) 430
XapI RAATTY 2 cut(s) 311, 537
XceI RCATGY 1 cut(s) 805
XcmI CCANNNNNNNNNTGG 1 cut(s) 431
XmiI GTMKAC 1 cut(s) 54
XspI CTAG 1 cut(s) 410
Zsp2I ATGCAT 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.