MD08G1209300.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0007393
Physical Location & Seq
Forward (+)
27198756 .. 27202369
3614 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1209300.v1.1.491

Sequence Viewer

Length: 528 bp
ATGGCCGGAGCAGCTTCCAACGACCAGGCTCTCTCTCTCCTTGCCACCGTCAACAACCACGGCGATTTGGCCGTCAAGCTCTCGTCTTTGAAGCAGGCTAAGGATCATTTGCTGTCCCTCGAGCCCTCTGGCTCAGCTCATAGAGGCCCAGCTTTAACACCCGCAGAAGTGTTGGTTTCCATTCATGGTATTGTTCCTGAGAATGATGGACTTGCACTTAAGAAGATAACAGATGCTTGCTCAGCCTGTTTTGAGCAAAGCACAGTTTTCACGCAGGCGTTGGCAAAGGCCCTGAATCAGATGGTTGATCAAACTCCTCTTCCTCTTCTCTTTATGAGAACAGTTTTTCAGGCAATAGATGATTTTCCTTCACTGGTTGATTTTGTTATGGAAATACTTTCCAAACTTGTGAGAAAACGGGTACGTGTGTCATCTACGCCTGTTATTTGGTGCTGTGTACTCTTTCAGGTTGACGTGTCAGTATTGGTTATTAACATGGCTAGGGGACAAGTGATGTTCTATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko04530 map03015 map04530
Pfam Domains
Protein Families
DUF3453 Symplekin_C

Protein Analysis

176

Amino Acids

18.97

Weight (kDa)

6.4

Isoelectric Point (pI)

30.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Symplekin_C PF12295 28 - 141 4.7e-32 Symplekin tight junction protein C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 162
AclWI GGATC 1 cut(s) 111
AcoI YGGCCR 2 cut(s) 3, 69
AfaI GTAC 2 cut(s) 423, 459
AflII CTTAAG 1 cut(s) 218
AflIII ACRYGT 2 cut(s) 424, 474
AgsI TTSAA 1 cut(s) 91
AjiI CACGTC 1 cut(s) 475
AjnI CCWGG 1 cut(s) 24
AluBI AGCT 4 cut(s) 14, 79, 137, 152
AluI AGCT 4 cut(s) 14, 79, 137, 152
AlwI GGATC 1 cut(s) 111
Ama87I CYCGRG 1 cut(s) 119
AoxI GGCC 4 cut(s) 3, 69, 145, 288
ApeKI GCWGC 1 cut(s) 11
AspS9I GGNCC 2 cut(s) 146, 289
AvaI CYCGRG 1 cut(s) 119
BaeI ACNNNNGTAYC 2 cut(s) 413, 446
BanII GRGCYC 1 cut(s) 126
BbvI GCAGC 1 cut(s) 23
BccI CCATC 2 cut(s) 200, 295
BceAI ACGGC 2 cut(s) 56, 76
BciT130I CCWGG 1 cut(s) 26
BclI TGATCA 1 cut(s) 307
BfaI CTAG 1 cut(s) 501
BfrI CTTAAG 1 cut(s) 218
BisI GCNGC 1 cut(s) 12
BlpI GCTNAGC 2 cut(s) 133, 241
BlsI GCNGC 1 cut(s) 13
Bme1390I CCNGG 1 cut(s) 26
BmeT110I CYCGRG 1 cut(s) 119
BmgBI CACGTC 1 cut(s) 475
BmgT120I GGNCC 2 cut(s) 146, 289
BmrFI CCNGG 1 cut(s) 26
BmsI GCATC 1 cut(s) 223
Bpu10I CCTNAGC 1 cut(s) 99
Bpu1102I GCTNAGC 2 cut(s) 133, 241
BsaAI YACGTR 1 cut(s) 425
BsaJI CCNNGG 1 cut(s) 58
Bse1I ACTGG 1 cut(s) 378
BseBI CCWGG 1 cut(s) 26
BseDI CCNNGG 1 cut(s) 58
BseMII CTCAG 3 cut(s) 147, 189, 255
BseNI ACTGG 1 cut(s) 378
BseRI GAGGAG 1 cut(s) 306
BseXI GCAGC 1 cut(s) 23
BseYI CCCAGC 1 cut(s) 148
BshFI GGCC 4 cut(s) 5, 71, 147, 290
BsiHKCI CYCGRG 1 cut(s) 119
BsiSI CCGG 1 cut(s) 6
BslFI GGGAC 2 cut(s) 100, 519
BsmFI GGGAC 2 cut(s) 100, 519
BsnI GGCC 4 cut(s) 5, 71, 147, 290
BsoBI CYCGRG 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 126
Bsp143I GATC 2 cut(s) 103, 307
Bsp1720I GCTNAGC 2 cut(s) 133, 241
BspACI CCGC 1 cut(s) 162
BspANI GGCC 4 cut(s) 5, 71, 147, 290
BspCNI CTCAG 3 cut(s) 146, 190, 254
BspPI GGATC 1 cut(s) 111
BspTI CTTAAG 1 cut(s) 218
BsrI ACTGG 1 cut(s) 378
BssECI CCNNGG 1 cut(s) 58
BssMI GATC 2 cut(s) 103, 307
Bst2UI CCWGG 1 cut(s) 26
Bst4CI ACNGT 3 cut(s) 49, 265, 343
Bst6I CTCTTC 2 cut(s) 324, 330
BstAFI CTTAAG 1 cut(s) 218
BstBAI YACGTR 1 cut(s) 425
BstC8I GCNNGC 3 cut(s) 96, 238, 276
BstDEI CTNAG 4 cut(s) 99, 133, 198, 241
BstDSI CCRYGG 1 cut(s) 58
BstKTI GATC 2 cut(s) 106, 310
BstMBI GATC 2 cut(s) 103, 307
BstMWI GCNNNNNNNGC 2 cut(s) 11, 242
BstNI CCWGG 1 cut(s) 26
BstSCI CCNGG 1 cut(s) 24
BstV1I GCAGC 1 cut(s) 23
BsuRI GGCC 4 cut(s) 5, 71, 147, 290
BtgI CCRYGG 1 cut(s) 58
BtrI CACGTC 1 cut(s) 475
BtsIMutI CAGTG 1 cut(s) 371
Cac8I GCNNGC 3 cut(s) 96, 238, 276
Cfr13I GGNCC 2 cut(s) 146, 289
Csp6I GTAC 2 cut(s) 422, 458
CviAII CATG 2 cut(s) 185, 496
CviQI GTAC 2 cut(s) 422, 458
DdeI CTNAG 4 cut(s) 99, 133, 198, 241
DpnI GATC 2 cut(s) 105, 309
DpnII GATC 2 cut(s) 103, 307
EaeI YGGCCR 2 cut(s) 3, 69
Eam1104I CTCTTC 2 cut(s) 324, 330
EarI CTCTTC 2 cut(s) 324, 330
Eco24I GRGCYC 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 119
EcoO109I RGGNCCY 1 cut(s) 289
EcoRII CCWGG 1 cut(s) 24
EcoT38I GRGCYC 1 cut(s) 126
FaeI CATG 2 cut(s) 188, 499
FaiI YATR 7 cut(s) 141, 186, 335, 389, 497, 522, 524
FaqI GGGAC 2 cut(s) 100, 519
FatI CATG 2 cut(s) 184, 495
FauI CCCGC 1 cut(s) 169
FbaI TGATCA 1 cut(s) 307
Fnu4HI GCNGC 1 cut(s) 12
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 1 cut(s) 12
FspBI CTAG 1 cut(s) 501
GluI GCNGC 1 cut(s) 12
GsaI CCCAGC 1 cut(s) 152
HaeIII GGCC 4 cut(s) 5, 71, 147, 290
HapII CCGG 1 cut(s) 6
Hin1II CATG 2 cut(s) 188, 499
HincII GTYRAC 2 cut(s) 52, 472
HindII GTYRAC 2 cut(s) 52, 472
HinfI GANTC 1 cut(s) 295
HpaII CCGG 1 cut(s) 6
Hpy166II GTNNAC 3 cut(s) 52, 458, 472
Hpy188I TCNGA 1 cut(s) 300
Hpy188III TCNNGA 1 cut(s) 197
Hpy8I GTNNAC 3 cut(s) 52, 458, 472
HpyAV CCTTC 1 cut(s) 378
HpyCH4III ACNGT 3 cut(s) 49, 265, 343
HpyCH4IV ACGT 2 cut(s) 424, 474
HpyCH4V TGCA 1 cut(s) 215
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 242
HpyF3I CTNAG 4 cut(s) 99, 133, 198, 241
HpySE526I ACGT 2 cut(s) 424, 474
Hsp92II CATG 2 cut(s) 188, 499
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 2 cut(s) 103, 307
LmnI GCTCC 1 cut(s) 8
Lsp1109I GCAGC 1 cut(s) 23
LweI GCATC 1 cut(s) 223
MaeI CTAG 1 cut(s) 501
MaeII ACGT 2 cut(s) 424, 474
MalI GATC 2 cut(s) 105, 309
MboI GATC 2 cut(s) 103, 307
MboII GAAGA 3 cut(s) 235, 311, 317
MhlI GDGCHC 1 cut(s) 126
MmeI TCCRAC 1 cut(s) 42
MnlI CCTC 5 cut(s) 128, 136, 137, 327, 333
MseI TTAA 3 cut(s) 155, 219, 492
MspCI CTTAAG 1 cut(s) 218
MspI CCGG 1 cut(s) 6
MspR9I CCNGG 1 cut(s) 26
MvaI CCWGG 1 cut(s) 26
MwoI GCNNNNNNNGC 2 cut(s) 11, 242
NdeII GATC 2 cut(s) 103, 307
NlaIII CATG 2 cut(s) 188, 499
PaeR7I CTCGAG 1 cut(s) 119
PfeI GAWTC 1 cut(s) 295
PkrI GCNGC 1 cut(s) 13
Ppu21I YACGTR 1 cut(s) 425
Psp6I CCWGG 1 cut(s) 24
PspFI CCCAGC 1 cut(s) 148
PspGI CCWGG 1 cut(s) 24
PspPI GGNCC 2 cut(s) 146, 289
PspXI VCTCGAGB 1 cut(s) 119
RsaI GTAC 2 cut(s) 423, 459
RsaNI GTAC 2 cut(s) 422, 458
SaqAI TTAA 3 cut(s) 155, 219, 492
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 2 cut(s) 103, 307
Sau96I GGNCC 2 cut(s) 146, 289
ScrFI CCNGG 1 cut(s) 26
SduI GDGCHC 1 cut(s) 126
SetI ASST 7 cut(s) 16, 81, 139, 154, 427, 471, 477
SfaNI GCATC 1 cut(s) 223
Sfr274I CTCGAG 1 cut(s) 119
SlaI CTCGAG 1 cut(s) 119
SmlI CTYRAG 2 cut(s) 119, 218
SmoI CTYRAG 2 cut(s) 119, 218
SsiI CCGC 1 cut(s) 162
SspMI CTAG 1 cut(s) 501
StyD4I CCNGG 1 cut(s) 24
TaaI ACNGT 3 cut(s) 49, 265, 343
TaiI ACGT 2 cut(s) 427, 477
TaqI TCGA 1 cut(s) 120
TatI WGTACW 1 cut(s) 457
TfiI GAWTC 1 cut(s) 295
Tru1I TTAA 3 cut(s) 155, 219, 492
Tru9I TTAA 3 cut(s) 155, 219, 492
TscAI CASTG 1 cut(s) 378
TseI GCWGC 1 cut(s) 11
TspDTI ATGAA 1 cut(s) 173
TspRI CASTG 1 cut(s) 378
Vha464I CTTAAG 1 cut(s) 218
XhoI CTCGAG 1 cut(s) 119
XspI CTAG 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.