Domain of unknown function (DUF3453)
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G01400 AT5G01400 AT5G01400 |
| fragaria_vesca | FvH4_6g11410 |
| malus_domestica | MD08G1209300.v1.1 MD12G1166800.v1.1 |
| prunus_persica | Prupe.6G277600_v2.0.a1 |
| pyrus_communis | pycom04g13880 pycom04g13890 pycom12g15880 |
| rosa_chinensis | RchiOBHm_Chr3g0463071 |
| rosa_laevigata | RLG00000024813 |
| rosa_multiflora | Rmu_co8095628.1_g000001 Rmu_sc0000766.1_g000036 Rmu_sc0008124.1_g000011 Rmu_sc0008124.1_g000012 Rmu_sc0008124.1_g000013 |
| rosa_roxburghii | Rroxscaffold_6G00416980 |
| rosa_rugosa | Rorug03G0061200 Rorug03G0061200 |
| rosa_samantha | Rh3AG119400 Rh3BG124000 Rh3CG125800 Rh3DG124900 |
| rosa_wichuraiana | Rw3G010100 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1605, 3644 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 68 |
| Acc16I | TGCGCA | 1 cut(s) | 3721 |
| Acc36I | ACCTGC | 2 cut(s) | 787, 3166 |
| AccB1I | GGYRCC | 1 cut(s) | 2083 |
| AccBSI | CCGCTC | 1 cut(s) | 2971 |
| AccI | GTMKAC | 1 cut(s) | 388 |
| AclWI | GGATC | 6 cut(s) | 542, 815, 2577, 3394, 3443, 3646 |
| AcoI | YGGCCR | 1 cut(s) | 1965 |
| AcsI | RAATTY | 6 cut(s) | 212, 512, 596, 646, 2491, 3465 |
| AcuI | CTGAAG | 7 cut(s) | 167, 365, 1473, 3278, 3441, 3734, 3743 |
| AcyI | GRCGYC | 3 cut(s) | 1155, 1598, 2084 |
| AdeI | CACNNNGTG | 1 cut(s) | 1187 |
| AfaI | GTAC | 9 cut(s) | 907, 1539, 1555, 1672, 2324, 2559, 2593, 3103, 3598 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 485, 3152, 3175 |
| AflIII | ACRYGT | 2 cut(s) | 850, 1419 |
| AgeI | ACCGGT | 1 cut(s) | 192 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 88, 2583 |
| AhlI | ACTAGT | 2 cut(s) | 335, 3094 |
| AjnI | CCWGG | 7 cut(s) | 478, 519, 912, 1444, 1816, 2246, 3345 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1335, 1367, 3803, 3835 |
| AleI | CACNNNNGTG | 1 cut(s) | 915 |
| Alw21I | GWGCWC | 4 cut(s) | 162, 865, 1123, 3002 |
| Alw26I | GTCTC | 8 cut(s) | 132, 720, 859, 1195, 1239, 1742, 1773, 2591 |
| Alw44I | GTGCAC | 1 cut(s) | 2998 |
| AlwI | GGATC | 6 cut(s) | 542, 815, 2577, 3394, 3443, 3646 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 1859, 1939, 2816 |
| Ama87I | CYCGRG | 1 cut(s) | 1503 |
| AoxI | GGCC | 3 cut(s) | 987, 1965, 2618 |
| ApaLI | GTGCAC | 1 cut(s) | 2998 |
| ApoI | RAATTY | 6 cut(s) | 212, 512, 596, 646, 2491, 3465 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 3725, 3757 |
| AseI | ATTAAT | 4 cut(s) | 288, 747, 840, 1011 |
| AsiGI | ACCGGT | 1 cut(s) | 192 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 528, 3892 |
| AspLEI | GCGC | 3 cut(s) | 1325, 2086, 3722 |
| AspS9I | GGNCC | 2 cut(s) | 1292, 3401 |
| AsuHPI | GGTGA | 9 cut(s) | 902, 1295, 1352, 1508, 1664, 1719, 1734, 2483, 2937 |
| AsuII | TTCGAA | 2 cut(s) | 2194, 2867 |
| AsuNHI | GCTAGC | 2 cut(s) | 1396, 3833 |
| AvaI | CYCGRG | 1 cut(s) | 1503 |
| AvaII | GGWCC | 2 cut(s) | 1292, 3401 |
| BaeGI | GKGCMC | 1 cut(s) | 3002 |
| BanI | GGYRCC | 1 cut(s) | 2083 |
| BanII | GRGCYC | 6 cut(s) | 162, 570, 680, 1065, 1824, 3113 |
| BauI | CACGAG | 3 cut(s) | 576, 2300, 3117 |
| BbsI | GAAGAC | 1 cut(s) | 1158 |
| Bbv12I | GWGCWC | 4 cut(s) | 162, 865, 1123, 3002 |
| BbvCI | CCTCAGC | 3 cut(s) | 1143, 1401, 2615 |
| BccI | CCATC | 9 cut(s) | 962, 1405, 1963, 2064, 2488, 2627, 2813, 3191, 3455 |
| BceAI | ACGGC | 2 cut(s) | 53, 262 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 1814, 1848, 1857, 1891, 2619, 2653 |
| BciT130I | CCWGG | 7 cut(s) | 480, 521, 914, 1446, 1818, 2248, 3347 |
| BciVI | GTATCC | 1 cut(s) | 840 |
| BclI | TGATCA | 1 cut(s) | 688 |
| BcoDI | GTCTC | 8 cut(s) | 132, 720, 859, 1195, 1239, 1742, 1773, 2591 |
| BcuI | ACTAGT | 2 cut(s) | 335, 3094 |
| BfmI | CTRYAG | 4 cut(s) | 2258, 2654, 3380, 3801 |
| BfoI | RGCGCY | 1 cut(s) | 2087 |
| BfuAI | ACCTGC | 2 cut(s) | 787, 3166 |
| BfuI | GTATCC | 1 cut(s) | 840 |
| BglII | AGATCT | 1 cut(s) | 3121 |
| BlpI | GCTNAGC | 1 cut(s) | 1985 |
| BmcAI | AGTACT | 1 cut(s) | 1555 |
| Bme1390I | CCNGG | 7 cut(s) | 480, 521, 914, 1446, 1818, 2248, 3347 |
| Bme18I | GGWCC | 2 cut(s) | 1292, 3401 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 88, 2583 |
| BmeT110I | CYCGRG | 1 cut(s) | 1503 |
| BmgT120I | GGNCC | 2 cut(s) | 1292, 3401 |
| BmiI | GGNNCC | 7 cut(s) | 559, 701, 1062, 2085, 2185, 2478, 2603 |
| BmrFI | CCNGG | 7 cut(s) | 480, 521, 914, 1446, 1818, 2248, 3347 |
| BmsI | GCATC | 9 cut(s) | 237, 1573, 1877, 1888, 2785, 2803, 3059, 3478, 3925 |
| BmtI | GCTAGC | 2 cut(s) | 1400, 3837 |
| BpiI | GAAGAC | 1 cut(s) | 1158 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 114, 146, 3641, 3673 |
| BpmI | CTGGAG | 7 cut(s) | 1026, 1467, 2696, 2862, 3132, 3399, 3753 |
| Bpu10I | CCTNAGC | 4 cut(s) | 1143, 1335, 1401, 2615 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1985 |
| Bpu14I | TTCGAA | 2 cut(s) | 2194, 2867 |
| BpuEI | CTTGAG | 5 cut(s) | 437, 1221, 1360, 2565, 3198 |
| BsaAI | YACGTR | 2 cut(s) | 253, 851 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1587, 3434 |
| BsaHI | GRCGYC | 3 cut(s) | 1155, 1598, 2084 |
| BsaI | GGTCTC | 2 cut(s) | 132, 2591 |
| BsaWI | WCCGGW | 3 cut(s) | 192, 1123, 3929 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 485, 3152, 3175 |
| Bse118I | RCCGGY | 4 cut(s) | 102, 192, 310, 2086 |
| Bse1I | ACTGG | 5 cut(s) | 998, 1043, 1717, 1902, 3636 |
| Bse3DI | GCAATG | 3 cut(s) | 680, 2736, 3502 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1587, 3434 |
| BseBI | CCWGG | 7 cut(s) | 480, 521, 914, 1446, 1818, 2248, 3347 |
| BseGI | GGATG | 6 cut(s) | 441, 625, 1153, 1894, 2094, 3389 |
| BseJI | GATNNNNATC | 2 cut(s) | 1587, 3434 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 485, 3152, 3175 |
| BseMI | GCAATG | 3 cut(s) | 680, 2736, 3502 |
| BseNI | ACTGG | 5 cut(s) | 998, 1043, 1717, 1902, 3636 |
| BseRI | GAGGAG | 3 cut(s) | 1073, 1994, 2896 |
| BseSI | GKGCMC | 1 cut(s) | 3002 |
| BseX3I | CGGCCG | 1 cut(s) | 1965 |
| BseYI | CCCAGC | 1 cut(s) | 571 |
| BsgI | GTGCAG | 5 cut(s) | 906, 1319, 1557, 2682, 3612 |
| Bsh1285I | CGRYCG | 4 cut(s) | 103, 193, 1968, 2119 |
| BshFI | GGCC | 3 cut(s) | 989, 1967, 2620 |
| BshNI | GGYRCC | 1 cut(s) | 2083 |
| BshTI | ACCGGT | 1 cut(s) | 192 |
| BsiEI | CGRYCG | 4 cut(s) | 103, 193, 1968, 2119 |
| BsiHKAI | GWGCWC | 4 cut(s) | 162, 865, 1123, 3002 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1503 |
| BsiSI | CCGG | 7 cut(s) | 103, 133, 193, 311, 1124, 2087, 3930 |
| BslFI | GGGAC | 2 cut(s) | 186, 2310 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 485, 3152, 3175 |
| BsmAI | GTCTC | 8 cut(s) | 132, 720, 859, 1195, 1239, 1742, 1773, 2591 |
| BsmBI | CGTCTC | 2 cut(s) | 1742, 1773 |
| BsmFI | GGGAC | 2 cut(s) | 186, 2310 |
| BsnI | GGCC | 3 cut(s) | 989, 1967, 2620 |
| Bso31I | GGTCTC | 2 cut(s) | 132, 2591 |
| BsoBI | CYCGRG | 1 cut(s) | 1503 |
| Bsp119I | TTCGAA | 2 cut(s) | 2194, 2867 |
| Bsp1286I | GDGCHC | 9 cut(s) | 162, 570, 680, 865, 1065, 1123, 1824, 3002, 3113 |
| Bsp1407I | TGTACA | 1 cut(s) | 2322 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1985 |
| Bsp19I | CCATGG | 1 cut(s) | 3439 |
| BspANI | GGCC | 3 cut(s) | 989, 1967, 2620 |
| BspLI | GGNNCC | 7 cut(s) | 559, 701, 1062, 2085, 2185, 2478, 2603 |
| BspMAI | CTGCAG | 2 cut(s) | 2262, 3805 |
| BspMI | ACCTGC | 2 cut(s) | 787, 3166 |
| BspOI | GCTAGC | 2 cut(s) | 1400, 3837 |
| BspPI | GGATC | 6 cut(s) | 542, 815, 2577, 3394, 3443, 3646 |
| BspQI | GCTCTTC | 1 cut(s) | 1626 |
| BspT104I | TTCGAA | 2 cut(s) | 2194, 2867 |
| BspT107I | GGYRCC | 1 cut(s) | 2083 |
| BspTNI | GGTCTC | 2 cut(s) | 132, 2591 |
| BsrBI | CCGCTC | 1 cut(s) | 2971 |
| BsrDI | GCAATG | 3 cut(s) | 680, 2736, 3502 |
| BsrFI | RCCGGY | 4 cut(s) | 102, 192, 310, 2086 |
| BsrGI | TGTACA | 1 cut(s) | 2322 |
| BsrI | ACTGG | 5 cut(s) | 998, 1043, 1717, 1902, 3636 |
| BssAI | RCCGGY | 4 cut(s) | 102, 192, 310, 2086 |
| BssNI | GRCGYC | 3 cut(s) | 1155, 1598, 2084 |
| BssSI | CACGAG | 3 cut(s) | 576, 2300, 3117 |
| BssT1I | CCWWGG | 5 cut(s) | 1990, 2232, 3439, 3819, 3863 |
| Bst2BI | CACGAG | 3 cut(s) | 576, 2300, 3117 |
| Bst2UI | CCWGG | 7 cut(s) | 480, 521, 914, 1446, 1818, 2248, 3347 |
| Bst6I | CTCTTC | 7 cut(s) | 179, 1050, 1626, 1776, 2103, 3411, 3788 |
| BstACI | GRCGYC | 3 cut(s) | 1155, 1598, 2084 |
| BstAPI | GCANNNNNTGC | 6 cut(s) | 869, 1331, 1939, 2003, 3181, 3497 |
| BstAUI | TGTACA | 1 cut(s) | 2322 |
| BstBAI | YACGTR | 2 cut(s) | 253, 851 |
| BstBI | TTCGAA | 2 cut(s) | 2194, 2867 |
| BstDSI | CCRYGG | 2 cut(s) | 926, 3439 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 3173 |
| BstF5I | GGATG | 6 cut(s) | 441, 625, 1153, 1894, 2094, 3389 |
| BstH2I | RGCGCY | 1 cut(s) | 2087 |
| BstHHI | GCGC | 3 cut(s) | 1325, 2086, 3722 |
| BstMAI | GTCTC | 8 cut(s) | 132, 720, 859, 1195, 1239, 1742, 1773, 2591 |
| BstMCI | CGRYCG | 4 cut(s) | 103, 193, 1968, 2119 |
| BstNI | CCWGG | 7 cut(s) | 480, 521, 914, 1446, 1818, 2248, 3347 |
| BstNSI | RCATGY | 6 cut(s) | 1396, 1423, 1671, 2521, 3495, 3504 |
| BstSCI | CCNGG | 7 cut(s) | 478, 519, 912, 1444, 1816, 2246, 3345 |
| BstSFI | CTRYAG | 4 cut(s) | 2258, 2654, 3380, 3801 |
| BstSLI | GKGCMC | 1 cut(s) | 3002 |
| BstV2I | GAAGAC | 1 cut(s) | 1158 |
| BstX2I | RGATCY | 2 cut(s) | 3121, 3651 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 3699 |
| BstYI | RGATCY | 2 cut(s) | 3121, 3651 |
| BstZI | CGGCCG | 1 cut(s) | 1965 |
| BsuI | GTATCC | 1 cut(s) | 840 |
| BsuRI | GGCC | 3 cut(s) | 989, 1967, 2620 |
| BtgI | CCRYGG | 2 cut(s) | 926, 3439 |
| BtsCI | GGATG | 6 cut(s) | 441, 625, 1153, 1894, 2094, 3389 |
| BtsI | GCAGTG | 2 cut(s) | 1937, 2889 |
| BtsIMutI | CAGTG | 4 cut(s) | 1937, 2889, 3188, 3629 |
| BveI | ACCTGC | 2 cut(s) | 787, 3166 |
| CaiI | CAGNNNCTG | 3 cut(s) | 1859, 1939, 2816 |
| CfoI | GCGC | 3 cut(s) | 1325, 2086, 3722 |
| Cfr10I | RCCGGY | 4 cut(s) | 102, 192, 310, 2086 |
| Cfr13I | GGNCC | 2 cut(s) | 1292, 3401 |
| CseI | GACGC | 2 cut(s) | 1163, 1587 |
| Csp6I | GTAC | 9 cut(s) | 906, 1538, 1554, 1671, 2323, 2558, 2592, 3102, 3597 |
| CspAI | ACCGGT | 1 cut(s) | 192 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1261, 1296 |
| CviQI | GTAC | 9 cut(s) | 906, 1538, 1554, 1671, 2323, 2558, 2592, 3102, 3597 |
| DinI | GGCGCC | 1 cut(s) | 2085 |
| DraIII | CACNNNGTG | 1 cut(s) | 1187 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 68 |
| DriI | GACNNNNNGTC | 2 cut(s) | 88, 2583 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 68 |
| EaeI | YGGCCR | 1 cut(s) | 1965 |
| EagI | CGGCCG | 1 cut(s) | 1965 |
| Eam1104I | CTCTTC | 7 cut(s) | 179, 1050, 1626, 1776, 2103, 3411, 3788 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 88, 2583 |
| EarI | CTCTTC | 7 cut(s) | 179, 1050, 1626, 1776, 2103, 3411, 3788 |
| EciI | GGCGGA | 2 cut(s) | 55, 1192 |
| Ecl136II | GAGCTC | 1 cut(s) | 160 |
| EclXI | CGGCCG | 1 cut(s) | 1965 |
| Eco130I | CCWWGG | 5 cut(s) | 1990, 2232, 3439, 3819, 3863 |
| Eco147I | AGGCCT | 1 cut(s) | 2620 |
| Eco24I | GRGCYC | 6 cut(s) | 162, 570, 680, 1065, 1824, 3113 |
| Eco31I | GGTCTC | 2 cut(s) | 132, 2591 |
| Eco32I | GATATC | 3 cut(s) | 2025, 2902, 3847 |
| Eco47I | GGWCC | 2 cut(s) | 1292, 3401 |
| Eco52I | CGGCCG | 1 cut(s) | 1965 |
| Eco53kI | GAGCTC | 1 cut(s) | 160 |
| Eco57I | CTGAAG | 7 cut(s) | 167, 365, 1473, 3278, 3441, 3734, 3743 |
| Eco88I | CYCGRG | 1 cut(s) | 1503 |
| EcoICRI | GAGCTC | 1 cut(s) | 160 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 3173 |
| EcoRI | GAATTC | 1 cut(s) | 3465 |
| EcoRII | CCWGG | 7 cut(s) | 478, 519, 912, 1444, 1816, 2246, 3345 |
| EcoRV | GATATC | 3 cut(s) | 2025, 2902, 3847 |
| EcoT14I | CCWWGG | 5 cut(s) | 1990, 2232, 3439, 3819, 3863 |
| EcoT22I | ATGCAT | 4 cut(s) | 3052, 3493, 3502, 3790 |
| EcoT38I | GRGCYC | 6 cut(s) | 162, 570, 680, 1065, 1824, 3113 |
| EgeI | GGCGCC | 1 cut(s) | 2085 |
| EheI | GGCGCC | 1 cut(s) | 2085 |
| ErhI | CCWWGG | 5 cut(s) | 1990, 2232, 3439, 3819, 3863 |
| Esp3I | CGTCTC | 2 cut(s) | 1742, 1773 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 516, 548 |
| FaqI | GGGAC | 2 cut(s) | 186, 2310 |
| FauI | CCCGC | 2 cut(s) | 151, 2150 |
| FbaI | TGATCA | 1 cut(s) | 688 |
| FblI | GTMKAC | 1 cut(s) | 388 |
| FokI | GGATG | 6 cut(s) | 448, 612, 1160, 1901, 2081, 3376 |
| FriOI | GRGCYC | 6 cut(s) | 162, 570, 680, 1065, 1824, 3113 |
| FspAI | RTGCGCAY | 1 cut(s) | 3721 |
| FspI | TGCGCA | 1 cut(s) | 3721 |
| GlaI | GCGC | 3 cut(s) | 1324, 2085, 3721 |
| GsaI | CCCAGC | 1 cut(s) | 575 |
| GsuI | CTGGAG | 7 cut(s) | 1026, 1467, 2696, 2862, 3132, 3399, 3753 |
| HaeII | RGCGCY | 1 cut(s) | 2087 |
| HaeIII | GGCC | 3 cut(s) | 989, 1967, 2620 |
| HapII | CCGG | 7 cut(s) | 103, 133, 193, 311, 1124, 2087, 3930 |
| HgaI | GACGC | 2 cut(s) | 1163, 1587 |
| HhaI | GCGC | 3 cut(s) | 1325, 2086, 3722 |
| Hin1I | GRCGYC | 3 cut(s) | 1155, 1598, 2084 |
| Hin6I | GCGC | 3 cut(s) | 1323, 2084, 3720 |
| HinP1I | GCGC | 3 cut(s) | 1323, 2084, 3720 |
| HincII | GTYRAC | 5 cut(s) | 954, 2014, 2052, 2307, 3729 |
| HindII | GTYRAC | 5 cut(s) | 954, 2014, 2052, 2307, 3729 |
| HindIII | AAGCTT | 1 cut(s) | 2717 |
| HpaII | CCGG | 7 cut(s) | 103, 133, 193, 311, 1124, 2087, 3930 |
| HphI | GGTGA | 9 cut(s) | 902, 1295, 1352, 1508, 1664, 1719, 1734, 2483, 2937 |
| Hpy99I | CGWCG | 3 cut(s) | 74, 77, 283 |
| HpyCH4IV | ACGT | 4 cut(s) | 252, 850, 2309, 2701 |
| HpySE526I | ACGT | 4 cut(s) | 252, 850, 2309, 2701 |
| Hsp92I | GRCGYC | 3 cut(s) | 1155, 1598, 2084 |
| HspAI | GCGC | 3 cut(s) | 1323, 2084, 3720 |
| KasI | GGCGCC | 1 cut(s) | 2083 |
| Ksp22I | TGATCA | 1 cut(s) | 688 |
| LguI | GCTCTTC | 1 cut(s) | 1626 |
| LweI | GCATC | 9 cut(s) | 237, 1573, 1877, 1888, 2785, 2803, 3059, 3478, 3925 |
| MaeII | ACGT | 4 cut(s) | 252, 850, 2309, 2701 |
| MaeIII | GTNAC | 6 cut(s) | 1639, 2575, 2943, 3114, 3481, 3961 |
| MbiI | CCGCTC | 1 cut(s) | 2971 |
| MfeI | CAATTG | 7 cut(s) | 113, 587, 654, 762, 2763, 2955, 3557 |
| MflI | RGATCY | 2 cut(s) | 3121, 3651 |
| MhlI | GDGCHC | 9 cut(s) | 162, 570, 680, 865, 1065, 1123, 1824, 3002, 3113 |
| Mly113I | GGCGCC | 1 cut(s) | 2084 |
| MlyI | GAGTC | 7 cut(s) | 56, 428, 1515, 1936, 2229, 2572, 2846 |
| MmeI | TCCRAC | 4 cut(s) | 60, 213, 384, 622 |
| Mph1103I | ATGCAT | 4 cut(s) | 3052, 3493, 3502, 3790 |
| MroXI | GAANNNNTTC | 2 cut(s) | 528, 3892 |
| MslI | CAYNNNNRTG | 6 cut(s) | 14, 224, 396, 737, 915, 2868 |
| MspA1I | CMGCKG | 3 cut(s) | 2000, 3152, 3419 |
| MspI | CCGG | 7 cut(s) | 103, 133, 193, 311, 1124, 2087, 3930 |
| MspR9I | CCNGG | 7 cut(s) | 480, 521, 914, 1446, 1818, 2248, 3347 |
| MunI | CAATTG | 7 cut(s) | 113, 587, 654, 762, 2763, 2955, 3557 |
| MvaI | CCWGG | 7 cut(s) | 480, 521, 914, 1446, 1818, 2248, 3347 |
| NarI | GGCGCC | 1 cut(s) | 2084 |
| NcoI | CCATGG | 1 cut(s) | 3439 |
| NheI | GCTAGC | 2 cut(s) | 1396, 3833 |
| NlaIV | GGNNCC | 7 cut(s) | 559, 701, 1062, 2085, 2185, 2478, 2603 |
| NmeAIII | GCCGAG | 2 cut(s) | 210, 2986 |
| NmuCI | GTSAC | 5 cut(s) | 2575, 2943, 3114, 3481, 3961 |
| NsbI | TGCGCA | 1 cut(s) | 3721 |
| NsiI | ATGCAT | 4 cut(s) | 3052, 3493, 3502, 3790 |
| NspI | RCATGY | 6 cut(s) | 1396, 1423, 1671, 2521, 3495, 3504 |
| NspV | TTCGAA | 2 cut(s) | 2194, 2867 |
| OliI | CACNNNNGTG | 1 cut(s) | 915 |
| PaeI | GCATGC | 1 cut(s) | 3495 |
| PaeR7I | CTCGAG | 1 cut(s) | 1503 |
| PceI | AGGCCT | 1 cut(s) | 2620 |
| PciI | ACATGT | 1 cut(s) | 1419 |
| PciSI | GCTCTTC | 1 cut(s) | 1626 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 78, 258 |
| PdmI | GAANNNNTTC | 2 cut(s) | 528, 3892 |
| PfoI | TCCNGGA | 2 cut(s) | 519, 1444 |
| PinAI | ACCGGT | 1 cut(s) | 192 |
| Ple19I | CGATCG | 1 cut(s) | 2119 |
| PleI | GAGTC | 7 cut(s) | 56, 427, 1514, 1935, 2228, 2572, 2846 |
| PluTI | GGCGCC | 1 cut(s) | 2087 |
| PpsI | GAGTC | 7 cut(s) | 56, 427, 1514, 1935, 2228, 2572, 2846 |
| Ppu21I | YACGTR | 2 cut(s) | 253, 851 |
| PscI | ACATGT | 1 cut(s) | 1419 |
| PshBI | ATTAAT | 4 cut(s) | 288, 747, 840, 1011 |
| PsiI | TTATAA | 2 cut(s) | 1605, 3644 |
| Psp124BI | GAGCTC | 1 cut(s) | 162 |
| Psp6I | CCWGG | 7 cut(s) | 478, 519, 912, 1444, 1816, 2246, 3345 |
| PspFI | CCCAGC | 1 cut(s) | 571 |
| PspGI | CCWGG | 7 cut(s) | 478, 519, 912, 1444, 1816, 2246, 3345 |
| PspN4I | GGNNCC | 7 cut(s) | 559, 701, 1062, 2085, 2185, 2478, 2603 |
| PspPI | GGNCC | 2 cut(s) | 1292, 3401 |
| PstI | CTGCAG | 2 cut(s) | 2262, 3805 |
| PstNI | CAGNNNCTG | 3 cut(s) | 1859, 1939, 2816 |
| PsuI | RGATCY | 2 cut(s) | 3121, 3651 |
| PvuI | CGATCG | 1 cut(s) | 2119 |
| PvuII | CAGCTG | 3 cut(s) | 2000, 3152, 3419 |
| RsaI | GTAC | 9 cut(s) | 907, 1539, 1555, 1672, 2324, 2559, 2593, 3103, 3598 |
| RsaNI | GTAC | 9 cut(s) | 906, 1538, 1554, 1671, 2323, 2558, 2592, 3102, 3597 |
| RseI | CAYNNNNRTG | 6 cut(s) | 14, 224, 396, 737, 915, 2868 |
| SacI | GAGCTC | 1 cut(s) | 162 |
| SapI | GCTCTTC | 1 cut(s) | 1626 |
| Sau96I | GGNCC | 2 cut(s) | 1292, 3401 |
| ScaI | AGTACT | 1 cut(s) | 1555 |
| SchI | GAGTC | 7 cut(s) | 56, 428, 1515, 1936, 2229, 2572, 2846 |
| ScrFI | CCNGG | 7 cut(s) | 480, 521, 914, 1446, 1818, 2248, 3347 |
| SduI | GDGCHC | 9 cut(s) | 162, 570, 680, 865, 1065, 1123, 1824, 3002, 3113 |
| SfaNI | GCATC | 9 cut(s) | 237, 1573, 1877, 1888, 2785, 2803, 3059, 3478, 3925 |
| SfcI | CTRYAG | 4 cut(s) | 2258, 2654, 3380, 3801 |
| SfoI | GGCGCC | 1 cut(s) | 2085 |
| Sfr274I | CTCGAG | 1 cut(s) | 1503 |
| SfuI | TTCGAA | 2 cut(s) | 2194, 2867 |
| SinI | GGWCC | 2 cut(s) | 1292, 3401 |
| SlaI | CTCGAG | 1 cut(s) | 1503 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 14, 224, 396, 737, 915, 2868 |
| SmlI | CTYRAG | 6 cut(s) | 416, 1236, 1375, 1503, 2580, 3213 |
| SmoI | CTYRAG | 6 cut(s) | 416, 1236, 1375, 1503, 2580, 3213 |
| SpeI | ACTAGT | 2 cut(s) | 335, 3094 |
| SphI | GCATGC | 1 cut(s) | 3495 |
| SseBI | AGGCCT | 1 cut(s) | 2620 |
| SspDI | GGCGCC | 1 cut(s) | 2083 |
| SspI | AATATT | 4 cut(s) | 459, 1009, 1225, 3030 |
| SstI | GAGCTC | 1 cut(s) | 162 |
| StuI | AGGCCT | 1 cut(s) | 2620 |
| StyD4I | CCNGG | 7 cut(s) | 478, 519, 912, 1444, 1816, 2246, 3345 |
| StyI | CCWWGG | 5 cut(s) | 1990, 2232, 3439, 3819, 3863 |
| TaiI | ACGT | 4 cut(s) | 255, 853, 2312, 2704 |
| TatI | WGTACW | 6 cut(s) | 905, 1537, 1553, 1670, 2322, 2557 |
| TauI | GCSGC | 1 cut(s) | 80 |
| TscAI | CASTG | 4 cut(s) | 1944, 2889, 3195, 3636 |
| TseFI | GTSAC | 5 cut(s) | 2575, 2943, 3114, 3481, 3961 |
| Tsp45I | GTSAC | 5 cut(s) | 2575, 2943, 3114, 3481, 3961 |
| TspGWI | ACGGA | 4 cut(s) | 1755, 1764, 1766, 2715 |
| TspRI | CASTG | 4 cut(s) | 1944, 2889, 3195, 3636 |
| VneI | GTGCAC | 1 cut(s) | 2998 |
| VpaK11BI | GGWCC | 2 cut(s) | 1292, 3401 |
| VspI | ATTAAT | 4 cut(s) | 288, 747, 840, 1011 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 3173 |
| XapI | RAATTY | 6 cut(s) | 212, 512, 596, 646, 2491, 3465 |
| XbaI | TCTAGA | 3 cut(s) | 364, 1579, 1906 |
| XceI | RCATGY | 6 cut(s) | 1396, 1423, 1671, 2521, 3495, 3504 |
| XhoI | CTCGAG | 1 cut(s) | 1503 |
| XmiI | GTMKAC | 1 cut(s) | 388 |
| XmnI | GAANNNNTTC | 2 cut(s) | 528, 3892 |
| ZrmI | AGTACT | 1 cut(s) | 1555 |
| Zsp2I | ATGCAT | 4 cut(s) | 3052, 3493, 3502, 3790 |