MD08G1213000.v1.1

Protein phosphatase methylesterase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012939
Physical Location & Seq
Forward (+)
27637774 .. 27645062
7289 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1213000.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGGATTCTTCAAACCTCACCTCGCTTCCGGAGGACACACTCGAAGACCAACAACAACAACCACCACCACAACCCCGACCTCTCTCTGCCTTTGCCTCTCGTCCCGATCGCCCTCCCACTCAGACTAGTTCAGAAAAGTACTCAGCTTTGGATTGGAAGGAGTATTTCGACACAGAGGATGATATCAGCATTCTGGGTTCGAATGATGTTTTTCATGTATACACGGCAGGAACGGAGGGACCAGTTGTGTTCTGTCTACACGGAGGTGGTTACTCAGGGCTCTCATTTGCCTTGTCAGCAAGTAAAATTAAGGAGAAAGCTCGGGTAGTTGCCATGGACTTGAGAGGACATGGAAAATCAACTACTGAAAATGATACCGATCTTTCTATTGAGACTATGTGCAATGATGTTGTAGCTGTTGTGAAAGCAATGTATGGAGATTCCCCTCCTGCAATTGTGCTTGTTGGCCACAGTATGGGAGGGTCAGTTGCTGTGCACATTGCTGCAAGGAAAGCATTGTCTAGTTTGGCTGGGCTGGTTGTTGTTGATGTTGTAGAGGGTACAGCTTTGGCTTCATTGGTTCATATGCAGAAAATCCTATCAGGCAGGGTGCAGCATTTTTCAAGCATTGAAAAAGCGATTGAATGGAGTATGAGGGGAGGGTCTTTAAGAAACATTGAGTCTGCTCGGGTGTCAATACCTTCCACATTAAAGTATGATGATTCAAAGAAATGTTACATTTATAGAGCACGACTTGAAGAAACAGAACAATATTGGAAAAGCTGGTATGAAGGCCTTTCAGAAAAGTTTTTGTCATCTCCCGTTCCAAAGATCCTGCTGTTAGCTGGAACAGACCGACTAGACAGAGCTCTGACAATTGGTCAAATGCAAGGGAAGTTCCAAATGGTTGTCATCAGACATACTGGGCATGCCATACAAGAAGATGTACCTGATGAATTTGCAACGCTAGTAACTAATTTTATATCCCGTAATCGCATAGGCCCTCGTGGAGTTGAGATTCCAGGACTCCGTCGGCCATCCCAGCCCAAACCTTGA

Protein Analysis

352

Amino Acids

38.51

Weight (kDa)

6.16

Isoelectric Point (pI)

44.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 81 - 315 3.9e-20 alpha/beta hydrolase fold
Hydrolase_4 PF12146 82 - 181 2.3e-12 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 83 - 322 1.2e-26 Alpha/beta hydrolase family
Thioesterase PF00975 83 - 184 3.7e-08 Thioesterase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012939)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10050 AT4G10050
fragaria_vesca FvH4_5g33360 FvH4_5g33360
malus_domestica MD08G1213000.v1.1 MD15G1395500.v1.1
prunus_persica Prupe.1G544300_v2.0.a1 Prupe.1G544300_v2.0.a1 Prupe.1G544300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0232091
rosa_laevigata RLG00000001392
rosa_multiflora Rmu_sc0000616.1_g000007 Rmu_sc0004618.1_g000006
rosa_roxburghii Rroxscaffold_3G00228820
rosa_rugosa Rorug07G0269000
rosa_samantha Rh7BG397600 Rh7CG443900
rosa_wichuraiana Rw7G035030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 475
AccI GTMKAC 2 cut(s) 219, 256
AccIII TCCGGA 1 cut(s) 28
AclWI GGATC 1 cut(s) 826
AcoI YGGCCR 2 cut(s) 466, 1034
AcsI RAATTY 1 cut(s) 956
AfaI GTAC 3 cut(s) 140, 562, 948
AfiI CCNNNNNNNGG 1 cut(s) 475
AgsI TTSAA 6 cut(s) 12, 624, 632, 644, 726, 758
AhdI GACNNNNNGTC 1 cut(s) 879
AhlI ACTAGT 1 cut(s) 125
AjnI CCWGG 1 cut(s) 1021
AleI CACNNNNGTG 1 cut(s) 264
AluBI AGCT 7 cut(s) 146, 320, 416, 566, 783, 845, 869
AluI AGCT 7 cut(s) 146, 320, 416, 566, 783, 845, 869
Alw21I GWGCWC 3 cut(s) 498, 751, 871
Alw26I GTCTC 1 cut(s) 386
Alw44I GTGCAC 1 cut(s) 494
AlwI GGATC 1 cut(s) 826
AlwNI CAGNNNCTG 1 cut(s) 491
Ama87I CYCGRG 2 cut(s) 321, 687
Aor13HI TCCGGA 1 cut(s) 28
AoxI GGCC 4 cut(s) 466, 793, 1000, 1034
ApaLI GTGCAC 1 cut(s) 494
ApeKI GCWGC 2 cut(s) 503, 613
ApoI RAATTY 1 cut(s) 956
AspS9I GGNCC 2 cut(s) 239, 1001
AsuHPI GGTGA 1 cut(s) 10
AsuII TTCGAA 1 cut(s) 200
AvaI CYCGRG 2 cut(s) 321, 687
AvaII GGWCC 1 cut(s) 239
BaeGI GKGCMC 1 cut(s) 498
BalI TGGCCA 1 cut(s) 468
BanII GRGCYC 2 cut(s) 282, 871
BauI CACGAG 1 cut(s) 1005
BbsI GAAGAC 1 cut(s) 51
Bbv12I GWGCWC 3 cut(s) 498, 751, 871
BbvI GCAGC 2 cut(s) 490, 625
BccI CCATC 1 cut(s) 1045
BceAI ACGGC 1 cut(s) 240
BciT130I CCWGG 1 cut(s) 1023
BcoDI GTCTC 1 cut(s) 386
BcuI ACTAGT 1 cut(s) 125
BfaI CTAG 4 cut(s) 126, 522, 860, 968
BisI GCNGC 2 cut(s) 504, 614
BlsI GCNGC 2 cut(s) 505, 615
BmcAI AGTACT 1 cut(s) 140
Bme1390I CCNGG 1 cut(s) 1023
Bme18I GGWCC 1 cut(s) 239
BmeRI GACNNNNNGTC 1 cut(s) 879
BmeT110I CYCGRG 2 cut(s) 321, 687
BmgT120I GGNCC 2 cut(s) 239, 1001
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 1 cut(s) 1023
BmrI ACTGGG 1 cut(s) 933
BmuI ACTGGG 1 cut(s) 933
BpiI GAAGAC 1 cut(s) 51
Bpu14I TTCGAA 1 cut(s) 200
BpuEI CTTGAG 1 cut(s) 361
BsaBI GATNNNNATC 1 cut(s) 378
BsaJI CCNNGG 1 cut(s) 333
BsaWI WCCGGW 1 cut(s) 28
BsaXI ACNNNNNCTCC 2 cut(s) 471, 501
Bsc4I CCNNNNNNNGG 1 cut(s) 475
Bse1I ACTGG 2 cut(s) 242, 928
Bse3DI GCAATG 3 cut(s) 409, 435, 498
Bse8I GATNNNNATC 1 cut(s) 378
BseAI TCCGGA 1 cut(s) 28
BseBI CCWGG 1 cut(s) 1023
BseDI CCNNGG 1 cut(s) 333
BseGI GGATG 2 cut(s) 184, 1037
BseJI GATNNNNATC 1 cut(s) 378
BseLI CCNNNNNNNGG 1 cut(s) 475
BseMI GCAATG 3 cut(s) 409, 435, 498
BseMII CTCAG 3 cut(s) 134, 156, 288
BseNI ACTGG 2 cut(s) 242, 928
BseSI GKGCMC 1 cut(s) 498
BseXI GCAGC 2 cut(s) 490, 625
BseYI CCCAGC 2 cut(s) 530, 1041
BsgI GTGCAG 1 cut(s) 632
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 4 cut(s) 468, 795, 1002, 1036
BsiEI CGRYCG 1 cut(s) 109
BsiHKAI GWGCWC 3 cut(s) 498, 751, 871
BsiHKCI CYCGRG 2 cut(s) 321, 687
BsiSI CCGG 1 cut(s) 29
BslFI GGGAC 2 cut(s) 87, 252
BslI CCNNNNNNNGG 1 cut(s) 475
BsmAI GTCTC 1 cut(s) 386
BsmFI GGGAC 2 cut(s) 87, 252
BsmI GAATGC 1 cut(s) 189
BsnI GGCC 4 cut(s) 468, 795, 1002, 1036
BsoBI CYCGRG 2 cut(s) 321, 687
Bsp119I TTCGAA 1 cut(s) 200
Bsp1286I GDGCHC 4 cut(s) 282, 498, 751, 871
Bsp13I TCCGGA 1 cut(s) 28
Bsp143I GATC 3 cut(s) 106, 379, 831
Bsp19I CCATGG 1 cut(s) 333
BspANI GGCC 4 cut(s) 468, 795, 1002, 1036
BspCNI CTCAG 3 cut(s) 133, 155, 287
BspEI TCCGGA 1 cut(s) 28
BspLI GGNNCC 1 cut(s) 240
BspPI GGATC 1 cut(s) 826
BspT104I TTCGAA 1 cut(s) 200
BsrDI GCAATG 3 cut(s) 409, 435, 498
BsrI ACTGG 2 cut(s) 242, 928
BssECI CCNNGG 1 cut(s) 333
BssMI GATC 3 cut(s) 106, 379, 831
BssNAI GTATAC 1 cut(s) 220
BssSI CACGAG 1 cut(s) 1005
BssT1I CCWWGG 1 cut(s) 333
Bst1107I GTATAC 1 cut(s) 220
Bst2BI CACGAG 1 cut(s) 1005
Bst2UI CCWGG 1 cut(s) 1023
Bst4CI ACNGT 1 cut(s) 473
BstBI TTCGAA 1 cut(s) 200
BstC8I GCNNGC 1 cut(s) 930
BstDEI CTNAG 3 cut(s) 120, 142, 274
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 2 cut(s) 184, 1037
BstKTI GATC 3 cut(s) 109, 382, 834
BstMAI GTCTC 1 cut(s) 386
BstMBI GATC 3 cut(s) 106, 379, 831
BstMCI CGRYCG 1 cut(s) 109
BstMWI GCNNNNNNNGC 3 cut(s) 296, 512, 1042
BstNI CCWGG 1 cut(s) 1023
BstNSI RCATGY 1 cut(s) 932
BstSCI CCNGG 1 cut(s) 1021
BstSLI GKGCMC 1 cut(s) 498
BstV1I GCAGC 2 cut(s) 490, 625
BstV2I GAAGAC 1 cut(s) 51
BstX2I RGATCY 1 cut(s) 831
BstYI RGATCY 1 cut(s) 831
BstZ17I GTATAC 1 cut(s) 220
BsuRI GGCC 4 cut(s) 468, 795, 1002, 1036
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 2 cut(s) 184, 1037
Cac8I GCNNGC 1 cut(s) 930
CaiI CAGNNNCTG 1 cut(s) 491
Cfr13I GGNCC 2 cut(s) 239, 1001
Csp6I GTAC 3 cut(s) 139, 561, 947
CviAII CATG 4 cut(s) 215, 334, 350, 929
CviQI GTAC 3 cut(s) 139, 561, 947
DdeI CTNAG 3 cut(s) 120, 142, 274
DpnI GATC 3 cut(s) 108, 381, 833
DpnII GATC 3 cut(s) 106, 379, 831
DriI GACNNNNNGTC 1 cut(s) 879
EaeI YGGCCR 2 cut(s) 466, 1034
Eam1105I GACNNNNNGTC 1 cut(s) 879
Ecl136II GAGCTC 1 cut(s) 869
Eco130I CCWWGG 1 cut(s) 333
Eco147I AGGCCT 1 cut(s) 795
Eco24I GRGCYC 2 cut(s) 282, 871
Eco32I GATATC 1 cut(s) 184
Eco47I GGWCC 1 cut(s) 239
Eco53kI GAGCTC 1 cut(s) 869
Eco88I CYCGRG 2 cut(s) 321, 687
EcoICRI GAGCTC 1 cut(s) 869
EcoO109I RGGNCCY 1 cut(s) 1001
EcoRII CCWGG 1 cut(s) 1021
EcoRV GATATC 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 333
EcoT38I GRGCYC 2 cut(s) 282, 871
ErhI CCWWGG 1 cut(s) 333
FaeI CATG 4 cut(s) 218, 337, 353, 932
FaqI GGGAC 2 cut(s) 87, 252
FatI CATG 4 cut(s) 214, 333, 349, 928
FauNDI CATATG 1 cut(s) 585
FblI GTMKAC 2 cut(s) 219, 256
Fnu4HI GCNGC 2 cut(s) 504, 614
FokI GGATG 2 cut(s) 191, 1024
FriOI GRGCYC 2 cut(s) 282, 871
Fsp4HI GCNGC 2 cut(s) 504, 614
FspBI CTAG 4 cut(s) 126, 522, 860, 968
GluI GCNGC 2 cut(s) 504, 614
GsaI CCCAGC 2 cut(s) 534, 1045
HaeIII GGCC 4 cut(s) 468, 795, 1002, 1036
HapII CCGG 1 cut(s) 29
Hin1II CATG 4 cut(s) 218, 337, 353, 932
HinfI GANTC 6 cut(s) 5, 440, 680, 722, 1018, 1026
HpaII CCGG 1 cut(s) 29
HphI GGTGA 1 cut(s) 10
Hpy166II GTNNAC 3 cut(s) 220, 257, 496
Hpy188I TCNGA 5 cut(s) 123, 133, 802, 873, 917
Hpy188III TCNNGA 2 cut(s) 29, 104
Hpy8I GTNNAC 3 cut(s) 220, 257, 496
Hpy99I CGWCG 1 cut(s) 1035
HpyAV CCTTC 3 cut(s) 151, 711, 785
HpyCH4III ACNGT 1 cut(s) 473
HpyCH4V TGCA 8 cut(s) 402, 452, 496, 506, 589, 613, 889, 962
HpyF10VI GCNNNNNNNGC 3 cut(s) 296, 512, 1042
HpyF3I CTNAG 3 cut(s) 120, 142, 274
Hsp92II CATG 4 cut(s) 218, 337, 353, 932
Kpn2I TCCGGA 1 cut(s) 28
Kzo9I GATC 3 cut(s) 106, 379, 831
Lsp1109I GCAGC 2 cut(s) 490, 625
MaeI CTAG 4 cut(s) 126, 522, 860, 968
MaeIII GTNAC 3 cut(s) 269, 734, 970
MalI GATC 3 cut(s) 108, 381, 833
MboI GATC 3 cut(s) 106, 379, 831
MboII GAAGA 3 cut(s) 56, 770, 953
MfeI CAATTG 2 cut(s) 453, 876
MflI RGATCY 1 cut(s) 831
MhlI GDGCHC 4 cut(s) 282, 498, 751, 871
MlsI TGGCCA 1 cut(s) 468
MluCI AATT 5 cut(s) 306, 453, 876, 956, 976
MluNI TGGCCA 1 cut(s) 468
MlyI GAGTC 2 cut(s) 689, 1020
Mox20I TGGCCA 1 cut(s) 468
MroI TCCGGA 1 cut(s) 28
MscI TGGCCA 1 cut(s) 468
MseI TTAA 3 cut(s) 309, 668, 710
MslI CAYNNNNRTG 1 cut(s) 264
Msp20I TGGCCA 1 cut(s) 468
MspI CCGG 1 cut(s) 29
MspR9I CCNGG 1 cut(s) 1023
MunI CAATTG 2 cut(s) 453, 876
Mva1269I GAATGC 1 cut(s) 189
MvaI CCWGG 1 cut(s) 1023
MwoI GCNNNNNNNGC 3 cut(s) 296, 512, 1042
NcoI CCATGG 1 cut(s) 333
NdeI CATATG 1 cut(s) 585
NdeII GATC 3 cut(s) 106, 379, 831
NlaIII CATG 4 cut(s) 218, 337, 353, 932
NlaIV GGNNCC 1 cut(s) 240
NspI RCATGY 1 cut(s) 932
NspV TTCGAA 1 cut(s) 200
OliI CACNNNNGTG 1 cut(s) 264
PaeI GCATGC 1 cut(s) 932
PceI AGGCCT 1 cut(s) 795
PctI GAATGC 1 cut(s) 189
PfeI GAWTC 4 cut(s) 5, 440, 722, 1018
PflFI GACNNNGTC 1 cut(s) 1029
PflMI CCANNNNNTGG 1 cut(s) 475
PfoI TCCNGGA 1 cut(s) 1021
PkrI GCNGC 2 cut(s) 505, 615
Ple19I CGATCG 1 cut(s) 109
PleI GAGTC 2 cut(s) 688, 1020
PpsI GAGTC 2 cut(s) 688, 1020
Psp124BI GAGCTC 1 cut(s) 871
Psp6I CCWGG 1 cut(s) 1021
PspFI CCCAGC 2 cut(s) 530, 1041
PspGI CCWGG 1 cut(s) 1021
PspN4I GGNNCC 1 cut(s) 240
PspPI GGNCC 2 cut(s) 239, 1001
PstNI CAGNNNCTG 1 cut(s) 491
PsuI RGATCY 1 cut(s) 831
PsyI GACNNNGTC 1 cut(s) 1029
PvuI CGATCG 1 cut(s) 109
RsaI GTAC 3 cut(s) 140, 562, 948
RsaNI GTAC 3 cut(s) 139, 561, 947
RseI CAYNNNNRTG 1 cut(s) 264
SacI GAGCTC 1 cut(s) 871
SaqAI TTAA 3 cut(s) 309, 668, 710
SatI GCNGC 2 cut(s) 504, 614
Sau3AI GATC 3 cut(s) 106, 379, 831
Sau96I GGNCC 2 cut(s) 239, 1001
ScaI AGTACT 1 cut(s) 140
SchI GAGTC 2 cut(s) 689, 1020
ScrFI CCNGG 1 cut(s) 1023
SduI GDGCHC 4 cut(s) 282, 498, 751, 871
SfuI TTCGAA 1 cut(s) 200
SinI GGWCC 1 cut(s) 239
SmiMI CAYNNNNRTG 1 cut(s) 264
SmlI CTYRAG 1 cut(s) 340
SmoI CTYRAG 1 cut(s) 340
SpeI ACTAGT 1 cut(s) 125
SphI GCATGC 1 cut(s) 932
Sse9I AATT 5 cut(s) 306, 453, 876, 956, 976
SseBI AGGCCT 1 cut(s) 795
SspI AATATT 1 cut(s) 773
SspMI CTAG 4 cut(s) 126, 522, 860, 968
SstI GAGCTC 1 cut(s) 871
StuI AGGCCT 1 cut(s) 795
StyD4I CCNGG 1 cut(s) 1021
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 1 cut(s) 473
TaqI TCGA 3 cut(s) 42, 168, 200
TaqII GACCGA 1 cut(s) 870
TasI AATT 5 cut(s) 306, 453, 876, 956, 976
TatI WGTACW 1 cut(s) 138
TfiI GAWTC 4 cut(s) 5, 440, 722, 1018
Tru1I TTAA 3 cut(s) 309, 668, 710
Tru9I TTAA 3 cut(s) 309, 668, 710
TseI GCWGC 2 cut(s) 503, 613
TspDTI ATGAA 5 cut(s) 203, 564, 572, 804, 969
TspGWI ACGGA 3 cut(s) 248, 276, 1019
Tth111I GACNNNGTC 1 cut(s) 1029
Van91I CCANNNNNTGG 1 cut(s) 475
VneI GTGCAC 1 cut(s) 494
VpaK11BI GGWCC 1 cut(s) 239
XapI RAATTY 1 cut(s) 956
XceI RCATGY 1 cut(s) 932
XmiI GTMKAC 2 cut(s) 219, 256
XspI CTAG 4 cut(s) 126, 522, 860, 968
ZrmI AGTACT 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.