Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0012627

Physical Location & Seq

Genomic Coordinates Reverse (-)

28069171 .. 28072164

Gene Structure 2994 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1218200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1848 bp

ATGGCTGAGGAACACCAGAAGCCTGAGGCTGCAGCTGCGCCAGAACCACCACCCAGCGAGGAAGTTGTCGTGGTGGCTGATGCCCCGCCTGCGGACAAGGCTGTGACCGAGAAAGAAGCGGTTCCGGTTCCAGAGCCGGAGGCCGAGGCAGAGAAGCCGGCCGTGACGACGACTGAGGAGGTGGAGGACAAAGCTGCCCCCAAGGCTGACGAGGATAAGAAAGTCATCGCTCAGTCGGTTTCCTTCAAGGAGGAGAGCTACGTTGTCGGGGAGCTTCCTGAGCCGCAGAAGAAGGCTCTTGACGAGCTGAAACAACTCATCCAGGAGGCTCTTAACAAGCACGAGTTCACTGCCCCGCCGCCCACCAAGGAGGAGGAGAAGCCTGTGGCGGCGGCGGCTGAGGAGAAAAAGGAGGAGGGGAAAACTGAAGAAGTACCACCCAAAGCTGAAGAAGAACCCAAAACGGAAGAAGCCTCCGCGTCCGTTACTGAAGCCAAAGAACAAGCAAAAGCAGAAGCAGTAGAAGAACCCAAAGAGGAAAAAAAACCAGAAGTAGAGGAAGAGCCGGTGGTTGTGACGGAGACTGTGGTGGAGAAAATTACAGAGACTGTTGATGATGACGGCGCCAAGACTGTGGAGGCGATTGAAGAGACGATCGTCGCAGTAGTCCCTGCTCCTGCTCCGGAGGCGGAGGCAGCCCCAGCTAAAGAGACCGAGGTGGCTGCTACAGAGGCCGCTCCCGCTGCTGCAGCTGCGGAGGAGACCAAAGCAGAAGCTGAGGCCCCAGCTGAGCCACCTGTAGCACCGGAGGAGGTGGACATATGGGGAATTCCGCTTCTGGGAGACGAAAGGAGCGACGTGGTTCTGTTGAAGTTCCTGAGGGCGAGGGATTTCAAGGTGAAGGAGGCGTTCTCCATGATCAAGAACACCGTGCGGTGGAGGAAGGAGTGGGGCATCGAAGGGCTGCTGGAGGAAGATCTGGGAAGCCACTGGGACAAGGTGGTGTTCACTCATGGGGTCGACAAGGAAGGCCATCCTGTGTGCTACAACGTGTTCGGGGAGTTCCAGAACAAGGAGTTGTACCAGAATACTTTTACGGACGAAGAGAAGCGTTCCAAGTTCATCAAGTGGAGGATCCAGTTCTTGGAGAAGAGCATCAGAAAGTTCGACTTCAATCCGACCGGGATTTCGACCATCGTTCAGGTCAATGATCTCAAGAACTTTCCTGGGTTTTTCAACAGGGAGCACAACCAGGTCACCAACCAGGCCCTTCAGCTGCTTCAGGATAACTACCCTGAGTTTGTAGCCAAACAGGTGTTTATCAATGTGCCATGGTGGTATCTTGCTTTTAATAGGATGATCAGCCCCTTCCTGACCCAGAGGACCAAGAGCAAGTTTGTATTTGCTGGTCCTTCCAAGTCTGCTGAAACCCTTTTCAAATATATAGGCCCAGAGCATGTGCCAGTCAAGTATGGTGGGCTAAGCAAGGAAGGTGTTGAAGGTGAGCACGAATTCACCACATCGGACCCTGTCACTGAGGTTACCGTCAAGCCAGCGTCCAAGCACACCGTTGAAATCCCTGTGTCTGAGTGTGGGGAGGTCTTGGTGTGGGAAGTGAGAGTGGTAGGGTGGGAAGTGAGCTATGGAGCTGAATTCGTGCCAAGCGCAGAGGACAGCTACACCATAATCTTGCAGAAGACAAGGAAGGTTGGAGCAGCGGATGAGCCGGTGATCTCAAACACCTACAAGATTGGCGAGGCGGGCAAGGTTGTGCTCACCATCGATAACCAAAGCTCCAAGAAGAAGAAGCTCCTCTACAGGTCCAAGACCAAGCCCTCCGAAATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0002020 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005794 GO:0005886 GO:0005911 GO:0008150 GO:0009506 GO:0009507 GO:0009536 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0019899 GO:0022414 GO:0030054 GO:0030154 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0048046 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0051704 GO:0055044 GO:0060560 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CRAL_TRIO CRAL_TRIO_N

Protein Analysis

616

Amino Acids

68.21

Weight (kDa)

4.75

Isoelectric Point (pI)

49.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CRAL_TRIO_N	PF03765	270 - 309	2.8e-09	CRAL/TRIO, N-terminal domain
CRAL_TRIO	PF00650	332 - 493	3.2e-30	CRAL/TRIO domain
GOLD_PATL1_C	PF25099	517 - 602	7.4e-38	Patellin-1-like, C-terminal GOLD domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012627)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g31750
malus_domestica	MD08G1218200.v1.1 MD15G1406000.v1.1
prunus_persica	Prupe.1G555200_v2.0.a1 Prupe.1G555200_v2.0.a1
pyrus_communis	pycom08g18910 pycom15g36280
rosa_chinensis	RchiOBHm_Chr7g0227641
rosa_laevigata	RLG00000001631
rosa_multiflora	Rmu_co8489989.1_g000001 Rmu_sc0041066.1_g000001
rosa_roxburghii	Rroxscaffold_3G00231990
rosa_rugosa	Rorug07G0242500 Rorug07G0242600
rosa_samantha	Rh7AG396100 Rh7BG377700 Rh7CG414800 Rh7DG391700
rosa_wichuraiana	Rw7G032930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	623
AccB7I	CCANNNNNTGG	1 cut(s)	1144
AccBSI	CCGCTC	1 cut(s)	737
AccI	GTMKAC	1 cut(s)	1020
AccII	CGCG	1 cut(s)	479
AccIII	TCCGGA	1 cut(s)	682
AclWI	GGATC	2 cut(s)	1129, 1142
AcoI	YGGCCR	1 cut(s)	159
AcsI	RAATTY	3 cut(s)	828, 1511, 1652
AcuI	CTGAAG	5 cut(s)	447, 468, 510, 1256, 1265
AcyI	GRCGYC	1 cut(s)	624
AfaI	GTAC	2 cut(s)	435, 1082
AfiI	CCNNNNNNNGG	5 cut(s)	91, 839, 936, 1072, 1144
AflIII	ACRYGT	1 cut(s)	1050
AgsI	TTSAA	9 cut(s)	247, 647, 871, 895, 1174, 1237, 1438, 1499, 1574
AjiI	CACGTC	1 cut(s)	859
AjnI	CCWGG	4 cut(s)	321, 1225, 1251, 1263
Alw21I	GWGCWC	3 cut(s)	1248, 1509, 1776
Alw26I	GTCTC	6 cut(s)	575, 599, 644, 704, 755, 837
AlwI	GGATC	2 cut(s)	1129, 1142
AlwNI	CAGNNNCTG	2 cut(s)	608, 776
Aor13HI	TCCGGA	1 cut(s)	682
AoxI	GGCC	7 cut(s)	141, 159, 732, 780, 1030, 1266, 1447
ApoI	RAATTY	3 cut(s)	828, 1511, 1652
ArsI	GACNNNNNNTTYG	2 cut(s)	1171, 1203
Asp700I	GAANNNNTTC	1 cut(s)	120
AspLEI	GCGC	3 cut(s)	40, 626, 1667
AspS9I	GGNCC	7 cut(s)	781, 1267, 1383, 1409, 1448, 1525, 1821
AsuC2I	CCSGG	1 cut(s)	1183
AsuHPI	GGTGA	6 cut(s)	910, 1249, 1507, 1514, 1741, 1768
AvaII	GGWCC	4 cut(s)	1383, 1409, 1525, 1821
AxyI	CCTNAGG	2 cut(s)	24, 878
BamHI	GGATCC	1 cut(s)	1134
BanI	GGYRCC	1 cut(s)	623
BarI	GAAGNNNNNNTAC	2 cut(s)	1799, 1831
BauI	CACGAG	1 cut(s)	341
BbsI	GAAGAC	1 cut(s)	1703
Bbv12I	GWGCWC	3 cut(s)	1248, 1509, 1776
BbvCI	CCTCAGC	3 cut(s)	6, 399, 777
BccI	CCATC	3 cut(s)	1041, 1202, 1787
BceAI	ACGGC	2 cut(s)	146, 637
BcgI	CGANNNNNNTGC	4 cut(s)	332, 366, 704, 738
BciT130I	CCWGG	4 cut(s)	323, 1227, 1253, 1265
BclI	TGATCA	2 cut(s)	918, 1359
BcnI	CCSGG	1 cut(s)	1183
BcoDI	GTCTC	6 cut(s)	575, 599, 644, 704, 755, 837
BfmI	CTRYAG	5 cut(s)	30, 726, 747, 798, 1816
BfoI	RGCGCY	1 cut(s)	627
BglI	GCCNNNNNGGC	1 cut(s)	203
BglII	AGATCT	1 cut(s)	976
BlpI	GCTNAGC	2 cut(s)	789, 1481
Bme1390I	CCNGG	5 cut(s)	323, 1183, 1227, 1253, 1265
Bme18I	GGWCC	4 cut(s)	1383, 1409, 1525, 1821
BmgBI	CACGTC	1 cut(s)	859
BmgT120I	GGNCC	7 cut(s)	781, 1267, 1383, 1409, 1448, 1525, 1821
BmiI	GGNNCC	6 cut(s)	123, 129, 625, 783, 1136, 1527
BmrFI	CCNGG	5 cut(s)	323, 1183, 1227, 1253, 1265
BmrI	ACTGGG	1 cut(s)	1000
BmsI	GCATC	3 cut(s)	70, 963, 1164
BmuI	ACTGGG	1 cut(s)	1000
BoxI	GACNNNNGTC	1 cut(s)	656
BpiI	GAAGAC	1 cut(s)	1703
BplI	GAGNNNNNCTC	2 cut(s)	896, 928
BpmI	CTGGAG	1 cut(s)	989
Bpu10I	CCTNAGC	4 cut(s)	6, 279, 399, 777
Bpu1102I	GCTNAGC	2 cut(s)	789, 1481
BpuEI	CTTGAG	1 cut(s)	1199
BpuMI	CCSGG	1 cut(s)	1183
Bsa29I	ATCGAT	1 cut(s)	1782
BsaHI	GRCGYC	1 cut(s)	624
BsaI	GGTCTC	2 cut(s)	704, 755
BsaJI	CCNNGG	6 cut(s)	144, 201, 366, 714, 1226, 1331
BsaWI	WCCGGW	3 cut(s)	124, 682, 805
BsaXI	ACNNNNNCTCC	2 cut(s)	1778, 1808
Bsc4I	CCNNNNNNNGG	5 cut(s)	91, 839, 936, 1072, 1144
Bse118I	RCCGGY	3 cut(s)	157, 565, 1726
Bse1I	ACTGG	3 cut(s)	995, 1138, 1463
Bse21I	CCTNAGG	2 cut(s)	24, 878
BseAI	TCCGGA	1 cut(s)	682
BseBI	CCWGG	4 cut(s)	323, 1227, 1253, 1265
BseCI	ATCGAT	1 cut(s)	1782
BseDI	CCNNGG	6 cut(s)	144, 201, 366, 714, 1226, 1331
BseGI	GGATG	4 cut(s)	318, 1033, 1362, 1726
BseLI	CCNNNNNNNGG	5 cut(s)	91, 839, 936, 1072, 1144
BseNI	ACTGG	3 cut(s)	995, 1138, 1463
BseRI	GAGGAG	9 cut(s)	191, 266, 386, 389, 416, 428, 773, 824, 1802
BseX3I	CGGCCG	1 cut(s)	159
BseYI	CCCAGC	3 cut(s)	53, 700, 784
Bsh1236I	CGCG	1 cut(s)	479
Bsh1285I	CGRYCG	3 cut(s)	162, 657, 1182
BshFI	GGCC	7 cut(s)	143, 161, 734, 782, 1032, 1268, 1449
BshNI	GGYRCC	1 cut(s)	623
BshVI	ATCGAT	1 cut(s)	1782
BsiEI	CGRYCG	3 cut(s)	162, 657, 1182
BsiHKAI	GWGCWC	3 cut(s)	1248, 1509, 1776
BsiSI	CCGG	8 cut(s)	125, 137, 158, 566, 683, 806, 1182, 1727
BslFI	GGGAC	2 cut(s)	653, 1007
BslI	CCNNNNNNNGG	5 cut(s)	91, 839, 936, 1072, 1144
BsmAI	GTCTC	6 cut(s)	575, 599, 644, 704, 755, 837
BsmBI	CGTCTC	2 cut(s)	644, 837
BsmFI	GGGAC	2 cut(s)	653, 1007
BsnI	GGCC	7 cut(s)	143, 161, 734, 782, 1032, 1268, 1449
Bso31I	GGTCTC	2 cut(s)	704, 755
Bsp1286I	GDGCHC	3 cut(s)	1248, 1509, 1776
Bsp13I	TCCGGA	1 cut(s)	682
Bsp143I	GATC	7 cut(s)	654, 918, 976, 1134, 1210, 1359, 1731
Bsp1720I	GCTNAGC	2 cut(s)	789, 1481
Bsp19I	CCATGG	1 cut(s)	1331
BspANI	GGCC	7 cut(s)	143, 161, 734, 782, 1032, 1268, 1449
BspDI	ATCGAT	1 cut(s)	1782
BspEI	TCCGGA	1 cut(s)	682
BspFNI	CGCG	1 cut(s)	479
BspLI	GGNNCC	6 cut(s)	123, 129, 625, 783, 1136, 1527
BspMAI	CTGCAG	2 cut(s)	34, 751
BspPI	GGATC	2 cut(s)	1129, 1142
BspQI	GCTCTTC	2 cut(s)	555, 1145
BspT107I	GGYRCC	1 cut(s)	623
BspTNI	GGTCTC	2 cut(s)	704, 755
BsrBI	CCGCTC	1 cut(s)	737
BsrFI	RCCGGY	3 cut(s)	157, 565, 1726
BsrI	ACTGG	3 cut(s)	995, 1138, 1463
BssAI	RCCGGY	3 cut(s)	157, 565, 1726
BssECI	CCNNGG	6 cut(s)	144, 201, 366, 714, 1226, 1331
BssMI	GATC	7 cut(s)	654, 918, 976, 1134, 1210, 1359, 1731
BssNI	GRCGYC	1 cut(s)	624
BssSI	CACGAG	1 cut(s)	341
BssT1I	CCWWGG	3 cut(s)	201, 366, 1331
Bst2BI	CACGAG	1 cut(s)	341
Bst2UI	CCWGG	4 cut(s)	323, 1227, 1253, 1265
Bst4CI	ACNGT	6 cut(s)	586, 610, 634, 931, 1546, 1570
Bst6I	CTCTTC	4 cut(s)	555, 642, 1098, 1145
BstACI	GRCGYC	1 cut(s)	624
BstC8I	GCNNGC	4 cut(s)	90, 159, 1554, 1762
BstDSI	CCRYGG	1 cut(s)	1331
BstEII	GGTNACC	2 cut(s)	1255, 1540
BstF5I	GGATG	4 cut(s)	318, 1033, 1362, 1726
BstFNI	CGCG	1 cut(s)	479
BstH2I	RGCGCY	1 cut(s)	627
BstHHI	GCGC	3 cut(s)	40, 626, 1667
BstKTI	GATC	7 cut(s)	657, 921, 979, 1137, 1213, 1362, 1734
BstMAI	GTCTC	6 cut(s)	575, 599, 644, 704, 755, 837
BstMBI	GATC	7 cut(s)	654, 918, 976, 1134, 1210, 1359, 1731
BstMCI	CGRYCG	3 cut(s)	162, 657, 1182
BstNI	CCWGG	4 cut(s)	323, 1227, 1253, 1265
BstNSI	RCATGY	1 cut(s)	1460
BstPAI	GACNNNNGTC	1 cut(s)	656
BstPI	GGTNACC	2 cut(s)	1255, 1540
BstSCI	CCNGG	5 cut(s)	321, 1181, 1225, 1251, 1263
BstSFI	CTRYAG	5 cut(s)	30, 726, 747, 798, 1816
BstUI	CGCG	1 cut(s)	479
BstV2I	GAAGAC	1 cut(s)	1703
BstX2I	RGATCY	2 cut(s)	976, 1134
BstXI	CCANNNNNNTGG	1 cut(s)	634
BstYI	RGATCY	2 cut(s)	976, 1134
BstZI	CGGCCG	1 cut(s)	159
Bsu15I	ATCGAT	1 cut(s)	1782
Bsu36I	CCTNAGG	2 cut(s)	24, 878
BsuRI	GGCC	7 cut(s)	143, 161, 734, 782, 1032, 1268, 1449
BsuTUI	ATCGAT	1 cut(s)	1782
BtgI	CCRYGG	1 cut(s)	1331
BtgZI	GCGATG	1 cut(s)	211
BtrI	CACGTC	1 cut(s)	859
BtsCI	GGATG	4 cut(s)	318, 1033, 1362, 1726
BtsI	GCAGTG	1 cut(s)	348
BtsIMutI	CAGTG	3 cut(s)	348, 988, 1533
Cac8I	GCNNGC	4 cut(s)	90, 159, 1554, 1762
CaiI	CAGNNNCTG	2 cut(s)	608, 776
CfoI	GCGC	3 cut(s)	40, 626, 1667
Cfr10I	RCCGGY	3 cut(s)	157, 565, 1726
Cfr13I	GGNCC	7 cut(s)	781, 1267, 1383, 1409, 1448, 1525, 1821
ClaI	ATCGAT	1 cut(s)	1782
CseI	GACGC	2 cut(s)	468, 1545
CsiI	ACCWGGT	1 cut(s)	1251
Csp6I	GTAC	2 cut(s)	434, 1081
CspCI	CAANNNNNGTGG	2 cut(s)	1456, 1491
CviAII	CATG	4 cut(s)	916, 1013, 1332, 1457
CviQI	GTAC	2 cut(s)	434, 1081
DinI	GGCGCC	1 cut(s)	625
DpnI	GATC	7 cut(s)	656, 920, 978, 1136, 1212, 1361, 1733
DpnII	GATC	7 cut(s)	654, 918, 976, 1134, 1210, 1359, 1731
EaeI	YGGCCR	1 cut(s)	159
EagI	CGGCCG	1 cut(s)	159
Eam1104I	CTCTTC	4 cut(s)	555, 642, 1098, 1145
EarI	CTCTTC	4 cut(s)	555, 642, 1098, 1145
EciI	GGCGGA	1 cut(s)	704
EclXI	CGGCCG	1 cut(s)	159
Eco130I	CCWWGG	3 cut(s)	201, 366, 1331
Eco31I	GGTCTC	2 cut(s)	704, 755
Eco47I	GGWCC	4 cut(s)	1383, 1409, 1525, 1821
Eco52I	CGGCCG	1 cut(s)	159
Eco57I	CTGAAG	5 cut(s)	447, 468, 510, 1256, 1265
Eco81I	CCTNAGG	2 cut(s)	24, 878
Eco91I	GGTNACC	2 cut(s)	1255, 1540
EcoO109I	RGGNCCY	2 cut(s)	781, 1267
EcoO65I	GGTNACC	2 cut(s)	1255, 1540
EcoRI	GAATTC	3 cut(s)	828, 1511, 1652
EcoRII	CCWGG	4 cut(s)	321, 1225, 1251, 1263
EcoT14I	CCWWGG	3 cut(s)	201, 366, 1331
EgeI	GGCGCC	1 cut(s)	625
EheI	GGCGCC	1 cut(s)	625
ErhI	CCWWGG	3 cut(s)	201, 366, 1331
Esp3I	CGTCTC	2 cut(s)	644, 837
FaeI	CATG	4 cut(s)	919, 1016, 1335, 1460
FalI	AAGNNNNNCTT	2 cut(s)	1154, 1186
FaqI	GGGAC	2 cut(s)	653, 1007
FatI	CATG	4 cut(s)	915, 1012, 1331, 1456
FauI	CCCGC	4 cut(s)	93, 363, 748, 1753
FauNDI	CATATG	1 cut(s)	821
FbaI	TGATCA	2 cut(s)	918, 1359
FblI	GTMKAC	1 cut(s)	1020
FokI	GGATG	4 cut(s)	305, 1020, 1369, 1733
GlaI	GCGC	3 cut(s)	39, 625, 1666
GsaI	CCCAGC	3 cut(s)	57, 704, 788
GsuI	CTGGAG	1 cut(s)	989
HaeII	RGCGCY	1 cut(s)	627
HaeIII	GGCC	7 cut(s)	143, 161, 734, 782, 1032, 1268, 1449
HapII	CCGG	8 cut(s)	125, 137, 158, 566, 683, 806, 1182, 1727
HgaI	GACGC	2 cut(s)	468, 1545
HhaI	GCGC	3 cut(s)	40, 626, 1667
Hin1I	GRCGYC	1 cut(s)	624
Hin1II	CATG	4 cut(s)	919, 1016, 1335, 1460
Hin6I	GCGC	3 cut(s)	38, 624, 1665
HinP1I	GCGC	3 cut(s)	38, 624, 1665
HincII	GTYRAC	1 cut(s)	1021
HindII	GTYRAC	1 cut(s)	1021
HpaII	CCGG	8 cut(s)	125, 137, 158, 566, 683, 806, 1182, 1727
HphI	GGTGA	6 cut(s)	910, 1249, 1507, 1514, 1741, 1768
Hpy166II	GTNNAC	4 cut(s)	348, 817, 1008, 1021
Hpy188I	TCNGA	6 cut(s)	1160, 1179, 1525, 1588, 1840, 1847
Hpy8I	GTNNAC	4 cut(s)	348, 817, 1008, 1021
Hpy99I	CGWCG	3 cut(s)	172, 662, 860
HpyCH4III	ACNGT	6 cut(s)	586, 610, 634, 931, 1546, 1570
HpyCH4IV	ACGT	3 cut(s)	261, 858, 1050
HpyCH4V	TGCA	3 cut(s)	32, 749, 1693
HpySE526I	ACGT	3 cut(s)	261, 858, 1050
Hsp92I	GRCGYC	1 cut(s)	624
Hsp92II	CATG	4 cut(s)	919, 1016, 1335, 1460
HspAI	GCGC	3 cut(s)	38, 624, 1665
KasI	GGCGCC	1 cut(s)	623
Kpn2I	TCCGGA	1 cut(s)	682
KroI	GCCGGC	1 cut(s)	157
KroNI	GCCGGC	1 cut(s)	159
Ksp22I	TGATCA	2 cut(s)	918, 1359
Kzo9I	GATC	7 cut(s)	654, 918, 976, 1134, 1210, 1359, 1731
LguI	GCTCTTC	2 cut(s)	555, 1145
LweI	GCATC	3 cut(s)	70, 963, 1164
MabI	ACCWGGT	1 cut(s)	1251
MaeII	ACGT	3 cut(s)	261, 858, 1050
MaeIII	GTNAC	7 cut(s)	103, 163, 484, 574, 1255, 1531, 1540
MalI	GATC	7 cut(s)	656, 920, 978, 1136, 1212, 1361, 1733
MbiI	CCGCTC	1 cut(s)	737
MboI	GATC	7 cut(s)	654, 918, 976, 1134, 1210, 1359, 1731
MflI	RGATCY	2 cut(s)	976, 1134
MhlI	GDGCHC	3 cut(s)	1248, 1509, 1776
MluCI	AATT	4 cut(s)	597, 828, 1511, 1652
Mly113I	GGCGCC	1 cut(s)	624
MmeI	TCCRAC	2 cut(s)	1202, 1690
MroI	TCCGGA	1 cut(s)	682
MroNI	GCCGGC	1 cut(s)	157
MroXI	GAANNNNTTC	1 cut(s)	120
MseI	TTAA	2 cut(s)	333, 1350
MspA1I	CMGCKG	6 cut(s)	35, 743, 752, 788, 1276, 1718
MspI	CCGG	8 cut(s)	125, 137, 158, 566, 683, 806, 1182, 1727
MspR9I	CCNGG	5 cut(s)	323, 1183, 1227, 1253, 1265
MvaI	CCWGG	4 cut(s)	323, 1227, 1253, 1265
MvnI	CGCG	1 cut(s)	479
NaeI	GCCGGC	1 cut(s)	159
NarI	GGCGCC	1 cut(s)	624
NciI	CCSGG	1 cut(s)	1183
NcoI	CCATGG	1 cut(s)	1331
NdeI	CATATG	1 cut(s)	821
NdeII	GATC	7 cut(s)	654, 918, 976, 1134, 1210, 1359, 1731
NgoMIV	GCCGGC	1 cut(s)	157
NlaIII	CATG	4 cut(s)	919, 1016, 1335, 1460
NlaIV	GGNNCC	6 cut(s)	123, 129, 625, 783, 1136, 1527
NmeAIII	GCCGAG	1 cut(s)	169
NmuCI	GTSAC	5 cut(s)	103, 163, 574, 1255, 1531
NspI	RCATGY	1 cut(s)	1460
PciSI	GCTCTTC	2 cut(s)	555, 1145
PcsI	WCGNNNNNNNCGW	1 cut(s)	852
PdiI	GCCGGC	1 cut(s)	159
PdmI	GAANNNNTTC	1 cut(s)	120
PflFI	GACNNNGTC	1 cut(s)	1529
PflMI	CCANNNNNTGG	1 cut(s)	1144
PfoI	TCCNGGA	1 cut(s)	321
Ple19I	CGATCG	1 cut(s)	657
PluTI	GGCGCC	1 cut(s)	627
PshAI	GACNNNNGTC	1 cut(s)	656
Psp6I	CCWGG	4 cut(s)	321, 1225, 1251, 1263
PspEI	GGTNACC	2 cut(s)	1255, 1540
PspFI	CCCAGC	3 cut(s)	53, 700, 784
PspGI	CCWGG	4 cut(s)	321, 1225, 1251, 1263
PspN4I	GGNNCC	6 cut(s)	123, 129, 625, 783, 1136, 1527
PspPI	GGNCC	7 cut(s)	781, 1267, 1383, 1409, 1448, 1525, 1821
PstI	CTGCAG	2 cut(s)	34, 751
PstNI	CAGNNNCTG	2 cut(s)	608, 776
PsuI	RGATCY	2 cut(s)	976, 1134
PsyI	GACNNNGTC	1 cut(s)	1529
PvuI	CGATCG	1 cut(s)	657
PvuII	CAGCTG	4 cut(s)	35, 752, 788, 1276
RsaI	GTAC	2 cut(s)	435, 1082
RsaNI	GTAC	2 cut(s)	434, 1081
SalI	GTCGAC	1 cut(s)	1019
SapI	GCTCTTC	2 cut(s)	555, 1145
SaqAI	TTAA	2 cut(s)	333, 1350
Sau3AI	GATC	7 cut(s)	654, 918, 976, 1134, 1210, 1359, 1731
Sau96I	GGNCC	7 cut(s)	781, 1267, 1383, 1409, 1448, 1525, 1821
ScrFI	CCNGG	5 cut(s)	323, 1183, 1227, 1253, 1265
SduI	GDGCHC	3 cut(s)	1248, 1509, 1776
SexAI	ACCWGGT	1 cut(s)	1251
SfaNI	GCATC	3 cut(s)	70, 963, 1164
SfcI	CTRYAG	5 cut(s)	30, 726, 747, 798, 1816
SfoI	GGCGCC	1 cut(s)	625
SinI	GGWCC	4 cut(s)	1383, 1409, 1525, 1821
SmlI	CTYRAG	1 cut(s)	1214
SmoI	CTYRAG	1 cut(s)	1214
Sse9I	AATT	4 cut(s)	597, 828, 1511, 1652
SspDI	GGCGCC	1 cut(s)	623
StyD4I	CCNGG	5 cut(s)	321, 1181, 1225, 1251, 1263
StyI	CCWWGG	3 cut(s)	201, 366, 1331
TaaI	ACNGT	6 cut(s)	586, 610, 634, 931, 1546, 1570
TaiI	ACGT	3 cut(s)	264, 861, 1053
TaqI	TCGA	5 cut(s)	957, 1020, 1167, 1190, 1782
TaqII	GACCGA	2 cut(s)	122, 728
TasI	AATT	4 cut(s)	597, 828, 1511, 1652
TauI	GCSGC	6 cut(s)	286, 361, 392, 395, 398, 737
Tru1I	TTAA	2 cut(s)	333, 1350
Tru9I	TTAA	2 cut(s)	333, 1350
TscAI	CASTG	3 cut(s)	355, 995, 1540
TseFI	GTSAC	5 cut(s)	103, 163, 574, 1255, 1531
Tsp45I	GTSAC	5 cut(s)	103, 163, 574, 1255, 1531
TspDTI	ATGAA	1 cut(s)	1111
TspGWI	ACGGA	4 cut(s)	472, 479, 593, 1112
TspRI	CASTG	3 cut(s)	355, 995, 1540
Tth111I	GACNNNGTC	1 cut(s)	1529
Van91I	CCANNNNNTGG	1 cut(s)	1144
VpaK11BI	GGWCC	4 cut(s)	1383, 1409, 1525, 1821
XapI	RAATTY	3 cut(s)	828, 1511, 1652
XceI	RCATGY	1 cut(s)	1460
XmiI	GTMKAC	1 cut(s)	1020
XmnI	GAANNNNTTC	1 cut(s)	120

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.