Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0012627

Physical Location & Seq

Genomic Coordinates Reverse (-)

50667774 .. 50670786

Gene Structure 3013 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1406000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1845 bp

ATGGCTGAGGAACACCAGAAGCCTGAGGCTGCAGCTGCGCCAGAAGCACCACCATCCGAGGAAGTTGTGGTGGTGGCTGATGCGCCGCCTGCCGATAAGTCTGTGACCGAGAAAGAAGCGGCTCCGGTTCCAGAGCCGGAGGCCGAGGCAGAGAAGCCGGCTGTGACGACGACTGAGGAGGTGGAGGACAAGGCTGCTCCCAAGGCTGACGAGGATCAGCAAGCCATCGCCCAGTCGGTTTCTTTCAAGGAAGAGAGCTACGTTGTTAGCGAGCTTCCCGAGCCTCAGAAGAAGGCTCTTGAGGAGCTGAAAGTACTCATCCAGGAGGCCCTTAATAAGCACGAGTTCACTGCTCCGCCGCCCGCCAAAGAGGAGGAGAAGCCTGTACCGGCGGCTGAGGAGAAGAAGGAGGAGGAGAAAACTGAAGAAGTACCACCCAAAGCTGAAGAGGAACCCAAAACGGAAGAAGCAAAAGCCGAAGCAGTAGAAGAAGCCAAAGAGGAAGAAAAGCCAAAAGAAGTGGAGGAGGAGAAGCCAAAAGAAGTGGAGGAGGAGAAGCCAAAAGCAGAGGAAGAGACGGTAGTTGTGACGGAGACTGTGGTGGAGAAAGTTACAGAGACTGTTGATGACGACGGCGCCAAGACTGTGGAGGCGATTGAAGAGACCATAGTCGCAGTAGCCCCTGCTCCGGCTGCAGAGGCCGAAGCAGCCCCATCTAAAGAGACCGAGGTGGCGGTGGAGGCTACTCCCGCTGCTGCTGCTGCTGCTGAGGAGGCCCCCGCGGCGCCACCTGTGGCGCCGGAGGAGGTGTACATATGGGGAATTCCGCTTCTGGGAGACGAAAGGAGCGACGTGGTTCTGTTGAAGTTCCTGAGGGCGAGGGATTTCAAGGTGAAGGAGGCGTTCGCCATGATCAAGAACAGCGTGCGGTGGAGGAAGGAGTGGGGCATCGAGGGGCTGCTGGAGGAAGATCTCGGAAGCCACTGGGACAAGGTGGTGTTCACTCATGGCGTCGACAAGGAAGGCCACCCTGTGTGCTACAACGTGTTCGGGGAGTTTCAGAACAAGGAGTTGTATCAGAATACTTTTACGGACGAAGAGAAGAGGGCCAAGTTCATCAAGTGGAGGATCCAGTTCTTGGAGAAGAGCATCAGAAAGTTCGACTTCAACCCGACCGGAATATCGACCATCGTTCAGGTCAATGATATCAAGAACTCTCCTGGGCTTTTCAAGAGGGAGCACAACCAGGTCACCAACCAGGCCCTTCATCTCCTTCAGGATAACTACCCTGAGTTTGTTGCCAGACAGGTGTTCATCAATGTGCCATGGTGGTATCTTGCTTTCAATAGGATGATCAGCCCCTTCCTGACCCAGAGGACCAAGAGCAAGTTTGTATTTGCTGGTCCTTCCAAGTCTGCTGAAACCCTTTTCAAATATATAGGCCCAGAGCATGTGCCAGTTCAGTATGGTGGGCTAAGCAAGGAAGGTGTTGAGGGTGAGCATGAATTCACCACATCTGACACTGTCACTGAGATTACTGTCAAGCCAGCATCCAAGCACACCGTCGAAATCCCCGTGTCTGAGTCTGGGGAGGTTTTGGTGTGGGAAGTGAGAGTGGTGGGATGGGAAGTAACCTATGGAGCTGAATTCGTGCCAAGCGCGGAGGACGGCTACACCATAATCTTACATAAGGCAAGGAAGGTCGGGGCAGCGGATGAGCCGGTGATCACAAACACGTACAAGATTGGCGAGGCGGGGAAGGTTGTGCTGACCATCGATAACCAAAGCTCGAAGAAGAAGAAGCTCCTCTACAGGTCCAAGACCAAGCCCTCCTCTGAAATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0002020 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005794 GO:0005886 GO:0005911 GO:0008150 GO:0009506 GO:0009507 GO:0009536 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0019899 GO:0022414 GO:0030054 GO:0030154 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0048046 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0051704 GO:0055044 GO:0060560 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CRAL_TRIO CRAL_TRIO_N

Protein Analysis

615

Amino Acids

68.25

Weight (kDa)

4.75

Isoelectric Point (pI)

50.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CRAL_TRIO_N	PF03765	272 - 307	3.6e-09	CRAL/TRIO, N-terminal domain
CRAL_TRIO	PF00650	330 - 491	3.9e-29	CRAL/TRIO domain
GOLD_PATL1_C	PF25099	515 - 600	7.4e-37	Patellin-1-like, C-terminal GOLD domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012627)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g31750
malus_domestica	MD08G1218200.v1.1 MD15G1406000.v1.1
prunus_persica	Prupe.1G555200_v2.0.a1 Prupe.1G555200_v2.0.a1
pyrus_communis	pycom08g18910 pycom15g36280
rosa_chinensis	RchiOBHm_Chr7g0227641
rosa_laevigata	RLG00000001631
rosa_multiflora	Rmu_co8489989.1_g000001 Rmu_sc0041066.1_g000001
rosa_roxburghii	Rroxscaffold_3G00231990
rosa_rugosa	Rorug07G0242500 Rorug07G0242600
rosa_samantha	Rh7AG396100 Rh7BG377700 Rh7CG414800 Rh7DG391700
rosa_wichuraiana	Rw7G032930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	635, 784, 796
AccB7I	CCANNNNNTGG	1 cut(s)	1138
AccI	GTMKAC	1 cut(s)	1014
AccII	CGCG	2 cut(s)	782, 1661
AclWI	GGATC	3 cut(s)	222, 1123, 1136
AcsI	RAATTY	3 cut(s)	822, 1505, 1646
AcuI	CTGAAG	3 cut(s)	444, 465, 1259
AcyI	GRCGYC	4 cut(s)	636, 785, 797, 1011
AdeI	CACNNNGTG	1 cut(s)	1033
AfaI	GTAC	5 cut(s)	315, 387, 432, 812, 1739
AfiI	CCNNNNNNNGG	3 cut(s)	688, 833, 1138
AflIII	ACRYGT	2 cut(s)	1044, 1734
AgsI	TTSAA	8 cut(s)	247, 659, 865, 889, 1168, 1231, 1345, 1432
AjiI	CACGTC	1 cut(s)	853
AjnI	CCWGG	4 cut(s)	321, 1219, 1245, 1257
AluBI	AGCT	8 cut(s)	35, 258, 274, 307, 443, 1643, 1788, 1804
AluI	AGCT	8 cut(s)	35, 258, 274, 307, 443, 1643, 1788, 1804
Alw21I	GWGCWC	1 cut(s)	1242
Alw26I	GTCTC	6 cut(s)	569, 587, 611, 656, 716, 831
AlwI	GGATC	3 cut(s)	222, 1123, 1136
AlwNI	CAGNNNCTG	1 cut(s)	620
Ama87I	CYCGRG	1 cut(s)	278
AoxI	GGCC	8 cut(s)	141, 327, 699, 774, 1024, 1107, 1260, 1441
ApoI	RAATTY	3 cut(s)	822, 1505, 1646
AspLEI	GCGC	6 cut(s)	40, 85, 638, 787, 799, 1661
AspS9I	GGNCC	8 cut(s)	328, 775, 1107, 1261, 1377, 1403, 1442, 1815
AsuHPI	GGTGA	5 cut(s)	904, 1243, 1501, 1508, 1735
AvaI	CYCGRG	1 cut(s)	278
AvaII	GGWCC	3 cut(s)	1377, 1403, 1815
AxyI	CCTNAGG	2 cut(s)	24, 872
BamHI	GGATCC	1 cut(s)	1128
BanI	GGYRCC	3 cut(s)	635, 784, 796
BarI	GAAGNNNNNNTAC	2 cut(s)	1793, 1825
BauI	CACGAG	1 cut(s)	341
Bbv12I	GWGCWC	1 cut(s)	1242
BbvCI	CCTCAGC	3 cut(s)	6, 396, 768
BccI	CCATC	6 cut(s)	61, 233, 721, 1196, 1617, 1781
BceAI	ACGGC	2 cut(s)	649, 1684
BcgI	CGANNNNNNTGC	2 cut(s)	332, 366
BciT130I	CCWGG	4 cut(s)	323, 1221, 1247, 1259
BclI	TGATCA	3 cut(s)	912, 1353, 1725
BcoDI	GTCTC	6 cut(s)	569, 587, 611, 656, 716, 831
BfmI	CTRYAG	3 cut(s)	30, 693, 1810
BfoI	RGCGCY	3 cut(s)	639, 788, 800
BglI	GCCNNNNNGGC	1 cut(s)	782
BglII	AGATCT	1 cut(s)	970
BlpI	GCTNAGC	1 cut(s)	1475
BmcAI	AGTACT	1 cut(s)	315
Bme1390I	CCNGG	4 cut(s)	323, 1221, 1247, 1259
Bme18I	GGWCC	3 cut(s)	1377, 1403, 1815
BmeT110I	CYCGRG	1 cut(s)	278
BmgBI	CACGTC	1 cut(s)	853
BmgT120I	GGNCC	8 cut(s)	328, 775, 1107, 1261, 1377, 1403, 1442, 1815
BmiI	GGNNCC	8 cut(s)	123, 129, 453, 637, 777, 786, 798, 1130
BmrFI	CCNGG	4 cut(s)	323, 1221, 1247, 1259
BmrI	ACTGGG	2 cut(s)	226, 994
BmsI	GCATC	4 cut(s)	70, 957, 1158, 1559
BmuI	ACTGGG	2 cut(s)	226, 994
BoxI	GACNNNNGTC	1 cut(s)	668
BpmI	CTGGAG	1 cut(s)	983
Bpu10I	CCTNAGC	3 cut(s)	6, 396, 768
Bpu1102I	GCTNAGC	1 cut(s)	1475
BpuEI	CTTGAG	1 cut(s)	320
Bsa29I	ATCGAT	1 cut(s)	1776
BsaAI	YACGTR	1 cut(s)	1737
BsaHI	GRCGYC	4 cut(s)	636, 785, 797, 1011
BsaI	GGTCTC	2 cut(s)	656, 716
BsaJI	CCNNGG	7 cut(s)	57, 144, 201, 726, 780, 1220, 1325
BsaWI	WCCGGW	2 cut(s)	124, 1175
BsaXI	ACNNNNNCTCC	2 cut(s)	794, 824
Bsc4I	CCNNNNNNNGG	3 cut(s)	688, 833, 1138
Bse118I	RCCGGY	3 cut(s)	157, 388, 1720
Bse1I	ACTGG	4 cut(s)	232, 989, 1132, 1457
Bse21I	CCTNAGG	2 cut(s)	24, 872
BseBI	CCWGG	4 cut(s)	323, 1221, 1247, 1259
BseCI	ATCGAT	1 cut(s)	1776
BseDI	CCNNGG	7 cut(s)	57, 144, 201, 726, 780, 1220, 1325
BseGI	GGATG	6 cut(s)	53, 318, 1356, 1550, 1628, 1720
BseLI	CCNNNNNNNGG	3 cut(s)	688, 833, 1138
BseMII	CTCAG	9 cut(s)	15, 165, 299, 387, 759, 863, 1281, 1521, 1572
BseNI	ACTGG	4 cut(s)	232, 989, 1132, 1457
Bsh1236I	CGCG	2 cut(s)	782, 1661
Bsh1285I	CGRYCG	1 cut(s)	1176
BshFI	GGCC	8 cut(s)	143, 329, 701, 776, 1026, 1109, 1262, 1443
BshNI	GGYRCC	3 cut(s)	635, 784, 796
BshVI	ATCGAT	1 cut(s)	1776
BsiEI	CGRYCG	1 cut(s)	1176
BsiHKAI	GWGCWC	1 cut(s)	1242
BsiHKCI	CYCGRG	1 cut(s)	278
BsiSI	CCGG	8 cut(s)	125, 137, 158, 389, 689, 800, 1176, 1721
BslFI	GGGAC	1 cut(s)	1001
BslI	CCNNNNNNNGG	3 cut(s)	688, 833, 1138
BsmAI	GTCTC	6 cut(s)	569, 587, 611, 656, 716, 831
BsmBI	CGTCTC	2 cut(s)	569, 831
BsmFI	GGGAC	1 cut(s)	1001
BsnI	GGCC	8 cut(s)	143, 329, 701, 776, 1026, 1109, 1262, 1443
Bso31I	GGTCTC	2 cut(s)	656, 716
BsoBI	CYCGRG	1 cut(s)	278
Bsp1286I	GDGCHC	1 cut(s)	1242
Bsp1407I	TGTACA	1 cut(s)	810
Bsp143I	GATC	6 cut(s)	214, 912, 970, 1128, 1353, 1725
Bsp1720I	GCTNAGC	1 cut(s)	1475
Bsp19I	CCATGG	1 cut(s)	1325
BspANI	GGCC	8 cut(s)	143, 329, 701, 776, 1026, 1109, 1262, 1443
BspCNI	CTCAG	9 cut(s)	16, 166, 298, 388, 760, 864, 1282, 1522, 1573
BspDI	ATCGAT	1 cut(s)	1776
BspFNI	CGCG	2 cut(s)	782, 1661
BspLI	GGNNCC	8 cut(s)	123, 129, 453, 637, 777, 786, 798, 1130
BspMAI	CTGCAG	2 cut(s)	34, 697
BspPI	GGATC	3 cut(s)	222, 1123, 1136
BspQI	GCTCTTC	1 cut(s)	1139
BspT107I	GGYRCC	3 cut(s)	635, 784, 796
BspTNI	GGTCTC	2 cut(s)	656, 716
BsrFI	RCCGGY	3 cut(s)	157, 388, 1720
BsrGI	TGTACA	1 cut(s)	810
BsrI	ACTGG	4 cut(s)	232, 989, 1132, 1457
BssAI	RCCGGY	3 cut(s)	157, 388, 1720
BssECI	CCNNGG	7 cut(s)	57, 144, 201, 726, 780, 1220, 1325
BssMI	GATC	6 cut(s)	214, 912, 970, 1128, 1353, 1725
BssNI	GRCGYC	4 cut(s)	636, 785, 797, 1011
BssSI	CACGAG	1 cut(s)	341
BssT1I	CCWWGG	2 cut(s)	201, 1325
Bst2BI	CACGAG	1 cut(s)	341
Bst2UI	CCWGG	4 cut(s)	323, 1221, 1247, 1259
Bst4CI	ACNGT	7 cut(s)	580, 598, 622, 646, 1525, 1540, 1564
Bst6I	CTCTTC	7 cut(s)	246, 441, 567, 654, 1092, 1097, 1139
BstACI	GRCGYC	4 cut(s)	636, 785, 797, 1011
BstAUI	TGTACA	1 cut(s)	810
BstBAI	YACGTR	1 cut(s)	1737
BstC8I	GCNNGC	7 cut(s)	90, 159, 222, 272, 363, 926, 1548
BstDSI	CCRYGG	2 cut(s)	780, 1325
BstEII	GGTNACC	1 cut(s)	1249
BstF5I	GGATG	6 cut(s)	53, 318, 1356, 1550, 1628, 1720
BstFNI	CGCG	2 cut(s)	782, 1661
BstH2I	RGCGCY	3 cut(s)	639, 788, 800
BstHHI	GCGC	6 cut(s)	40, 85, 638, 787, 799, 1661
BstKTI	GATC	6 cut(s)	217, 915, 973, 1131, 1356, 1728
BstMAI	GTCTC	6 cut(s)	569, 587, 611, 656, 716, 831
BstMBI	GATC	6 cut(s)	214, 912, 970, 1128, 1353, 1725
BstMCI	CGRYCG	1 cut(s)	1176
BstNI	CCWGG	4 cut(s)	323, 1221, 1247, 1259
BstNSI	RCATGY	1 cut(s)	1454
BstPAI	GACNNNNGTC	1 cut(s)	668
BstPI	GGTNACC	1 cut(s)	1249
BstSCI	CCNGG	4 cut(s)	321, 1219, 1245, 1257
BstSFI	CTRYAG	3 cut(s)	30, 693, 1810
BstUI	CGCG	2 cut(s)	782, 1661
BstX2I	RGATCY	2 cut(s)	970, 1128
BstXI	CCANNNNNNTGG	1 cut(s)	646
BstYI	RGATCY	2 cut(s)	970, 1128
Bsu15I	ATCGAT	1 cut(s)	1776
Bsu36I	CCTNAGG	2 cut(s)	24, 872
BsuRI	GGCC	8 cut(s)	143, 329, 701, 776, 1026, 1109, 1262, 1443
BsuTUI	ATCGAT	1 cut(s)	1776
BtgI	CCRYGG	2 cut(s)	780, 1325
BtgZI	GCGATG	1 cut(s)	211
BtrI	CACGTC	1 cut(s)	853
BtsCI	GGATG	6 cut(s)	53, 318, 1356, 1550, 1628, 1720
BtsI	GCAGTG	1 cut(s)	348
BtsIMutI	CAGTG	4 cut(s)	348, 982, 1521, 1527
Cac8I	GCNNGC	7 cut(s)	90, 159, 222, 272, 363, 926, 1548
CaiI	CAGNNNCTG	1 cut(s)	620
CfoI	GCGC	6 cut(s)	40, 85, 638, 787, 799, 1661
Cfr10I	RCCGGY	3 cut(s)	157, 388, 1720
Cfr13I	GGNCC	8 cut(s)	328, 775, 1107, 1261, 1377, 1403, 1442, 1815
Cfr42I	CCGCGG	1 cut(s)	783
ClaI	ATCGAT	1 cut(s)	1776
CseI	GACGC	1 cut(s)	1000
CsiI	ACCWGGT	1 cut(s)	1245
Csp6I	GTAC	5 cut(s)	314, 386, 431, 811, 1738
CspCI	CAANNNNNGTGG	4 cut(s)	501, 525, 536, 560
CviAII	CATG	5 cut(s)	910, 1007, 1326, 1451, 1502
CviQI	GTAC	5 cut(s)	314, 386, 431, 811, 1738
DinI	GGCGCC	3 cut(s)	637, 786, 798
DpnI	GATC	6 cut(s)	216, 914, 972, 1130, 1355, 1727
DpnII	GATC	6 cut(s)	214, 912, 970, 1128, 1353, 1725
DraIII	CACNNNGTG	1 cut(s)	1033
Eam1104I	CTCTTC	7 cut(s)	246, 441, 567, 654, 1092, 1097, 1139
EarI	CTCTTC	7 cut(s)	246, 441, 567, 654, 1092, 1097, 1139
EciI	GGCGGA	1 cut(s)	345
Eco130I	CCWWGG	2 cut(s)	201, 1325
Eco31I	GGTCTC	2 cut(s)	656, 716
Eco32I	GATATC	1 cut(s)	1207
Eco47I	GGWCC	3 cut(s)	1377, 1403, 1815
Eco57I	CTGAAG	3 cut(s)	444, 465, 1259
Eco81I	CCTNAGG	2 cut(s)	24, 872
Eco88I	CYCGRG	1 cut(s)	278
Eco91I	GGTNACC	1 cut(s)	1249
EcoO109I	RGGNCCY	3 cut(s)	328, 775, 1261
EcoO65I	GGTNACC	1 cut(s)	1249
EcoRI	GAATTC	3 cut(s)	822, 1505, 1646
EcoRII	CCWGG	4 cut(s)	321, 1219, 1245, 1257
EcoRV	GATATC	1 cut(s)	1207
EcoT14I	CCWWGG	2 cut(s)	201, 1325
EgeI	GGCGCC	3 cut(s)	637, 786, 798
EheI	GGCGCC	3 cut(s)	637, 786, 798
ErhI	CCWWGG	2 cut(s)	201, 1325
Esp3I	CGTCTC	2 cut(s)	569, 831
FaeI	CATG	5 cut(s)	913, 1010, 1329, 1454, 1505
FalI	AAGNNNNNCTT	2 cut(s)	1148, 1180
FaqI	GGGAC	1 cut(s)	1001
FatI	CATG	5 cut(s)	909, 1006, 1325, 1450, 1501
FauI	CCCGC	4 cut(s)	370, 757, 787, 1747
FauNDI	CATATG	1 cut(s)	815
FbaI	TGATCA	3 cut(s)	912, 1353, 1725
FblI	GTMKAC	1 cut(s)	1014
FokI	GGATG	6 cut(s)	40, 305, 1363, 1537, 1635, 1727
GlaI	GCGC	6 cut(s)	39, 84, 637, 786, 798, 1660
GsuI	CTGGAG	1 cut(s)	983
HaeII	RGCGCY	3 cut(s)	639, 788, 800
HaeIII	GGCC	8 cut(s)	143, 329, 701, 776, 1026, 1109, 1262, 1443
HapII	CCGG	8 cut(s)	125, 137, 158, 389, 689, 800, 1176, 1721
HgaI	GACGC	1 cut(s)	1000
HhaI	GCGC	6 cut(s)	40, 85, 638, 787, 799, 1661
Hin1I	GRCGYC	4 cut(s)	636, 785, 797, 1011
Hin1II	CATG	5 cut(s)	913, 1010, 1329, 1454, 1505
Hin6I	GCGC	6 cut(s)	38, 83, 636, 785, 797, 1659
HinP1I	GCGC	6 cut(s)	38, 83, 636, 785, 797, 1659
HincII	GTYRAC	1 cut(s)	1015
HindII	GTYRAC	1 cut(s)	1015
HinfI	GANTC	1 cut(s)	1583
HpaII	CCGG	8 cut(s)	125, 137, 158, 389, 689, 800, 1176, 1721
HphI	GGTGA	5 cut(s)	904, 1243, 1501, 1508, 1735
Hpy166II	GTNNAC	4 cut(s)	348, 811, 1002, 1015
Hpy188III	TCNNGA	9 cut(s)	131, 278, 299, 871, 916, 1210, 1231, 1277, 1366
Hpy8I	GTNNAC	4 cut(s)	348, 811, 1002, 1015
Hpy99I	CGWCG	5 cut(s)	172, 635, 854, 1016, 1568
HpyCH4III	ACNGT	7 cut(s)	580, 598, 622, 646, 1525, 1540, 1564
HpyCH4IV	ACGT	4 cut(s)	261, 852, 1044, 1736
HpyCH4V	TGCA	2 cut(s)	32, 695
HpySE526I	ACGT	4 cut(s)	261, 852, 1044, 1736
Hsp92I	GRCGYC	4 cut(s)	636, 785, 797, 1011
Hsp92II	CATG	5 cut(s)	913, 1010, 1329, 1454, 1505
HspAI	GCGC	6 cut(s)	38, 83, 636, 785, 797, 1659
KasI	GGCGCC	3 cut(s)	635, 784, 796
KroI	GCCGGC	1 cut(s)	157
KroNI	GCCGGC	1 cut(s)	159
Ksp22I	TGATCA	3 cut(s)	912, 1353, 1725
KspI	CCGCGG	1 cut(s)	783
Kzo9I	GATC	6 cut(s)	214, 912, 970, 1128, 1353, 1725
LguI	GCTCTTC	1 cut(s)	1139
LmnI	GCTCC	9 cut(s)	127, 202, 304, 358, 691, 846, 1237, 1640, 1809
LweI	GCATC	4 cut(s)	70, 957, 1158, 1559
MabI	ACCWGGT	1 cut(s)	1245
MaeII	ACGT	4 cut(s)	261, 852, 1044, 1736
MaeIII	GTNAC	7 cut(s)	103, 163, 586, 610, 1249, 1525, 1630
MalI	GATC	6 cut(s)	216, 914, 972, 1130, 1355, 1727
MboI	GATC	6 cut(s)	214, 912, 970, 1128, 1353, 1725
MflI	RGATCY	2 cut(s)	970, 1128
MhlI	GDGCHC	1 cut(s)	1242
MluCI	AATT	3 cut(s)	822, 1505, 1646
Mly113I	GGCGCC	3 cut(s)	636, 785, 797
MlyI	GAGTC	1 cut(s)	1592
MroNI	GCCGGC	1 cut(s)	157
MseI	TTAA	1 cut(s)	333
MslI	CAYNNNNRTG	1 cut(s)	1319
MspA1I	CMGCKG	4 cut(s)	35, 752, 782, 1712
MspI	CCGG	8 cut(s)	125, 137, 158, 389, 689, 800, 1176, 1721
MspR9I	CCNGG	4 cut(s)	323, 1221, 1247, 1259
MvaI	CCWGG	4 cut(s)	323, 1221, 1247, 1259
MvnI	CGCG	2 cut(s)	782, 1661
NaeI	GCCGGC	1 cut(s)	159
NarI	GGCGCC	3 cut(s)	636, 785, 797
NcoI	CCATGG	1 cut(s)	1325
NdeI	CATATG	1 cut(s)	815
NdeII	GATC	6 cut(s)	214, 912, 970, 1128, 1353, 1725
NgoMIV	GCCGGC	1 cut(s)	157
NlaIII	CATG	5 cut(s)	913, 1010, 1329, 1454, 1505
NlaIV	GGNNCC	8 cut(s)	123, 129, 453, 637, 777, 786, 798, 1130
NmeAIII	GCCGAG	1 cut(s)	169
NmuCI	GTSAC	5 cut(s)	103, 163, 586, 1249, 1525
NspI	RCATGY	1 cut(s)	1454
PciSI	GCTCTTC	1 cut(s)	1139
PcsI	WCGNNNNNNNCGW	3 cut(s)	267, 846, 1572
PdiI	GCCGGC	1 cut(s)	159
PflFI	GACNNNGTC	1 cut(s)	1523
PflMI	CCANNNNNTGG	1 cut(s)	1138
PfoI	TCCNGGA	1 cut(s)	321
PleI	GAGTC	1 cut(s)	1591
PluTI	GGCGCC	3 cut(s)	639, 788, 800
PpsI	GAGTC	1 cut(s)	1591
Ppu21I	YACGTR	1 cut(s)	1737
PshAI	GACNNNNGTC	1 cut(s)	668
Psp6I	CCWGG	4 cut(s)	321, 1219, 1245, 1257
PspEI	GGTNACC	1 cut(s)	1249
PspGI	CCWGG	4 cut(s)	321, 1219, 1245, 1257
PspN4I	GGNNCC	8 cut(s)	123, 129, 453, 637, 777, 786, 798, 1130
PspPI	GGNCC	8 cut(s)	328, 775, 1107, 1261, 1377, 1403, 1442, 1815
PstI	CTGCAG	2 cut(s)	34, 697
PstNI	CAGNNNCTG	1 cut(s)	620
PsuI	RGATCY	2 cut(s)	970, 1128
PsyI	GACNNNGTC	1 cut(s)	1523
PvuII	CAGCTG	1 cut(s)	35
RsaI	GTAC	5 cut(s)	315, 387, 432, 812, 1739
RsaNI	GTAC	5 cut(s)	314, 386, 431, 811, 1738
RseI	CAYNNNNRTG	1 cut(s)	1319
SacII	CCGCGG	1 cut(s)	783
SalI	GTCGAC	1 cut(s)	1013
SapI	GCTCTTC	1 cut(s)	1139
SaqAI	TTAA	1 cut(s)	333
Sau3AI	GATC	6 cut(s)	214, 912, 970, 1128, 1353, 1725
Sau96I	GGNCC	8 cut(s)	328, 775, 1107, 1261, 1377, 1403, 1442, 1815
ScaI	AGTACT	1 cut(s)	315
SchI	GAGTC	1 cut(s)	1592
ScrFI	CCNGG	4 cut(s)	323, 1221, 1247, 1259
SduI	GDGCHC	1 cut(s)	1242
SexAI	ACCWGGT	1 cut(s)	1245
SfaNI	GCATC	4 cut(s)	70, 957, 1158, 1559
SfcI	CTRYAG	3 cut(s)	30, 693, 1810
SfoI	GGCGCC	3 cut(s)	637, 786, 798
Sfr303I	CCGCGG	1 cut(s)	783
SgrBI	CCGCGG	1 cut(s)	783
SinI	GGWCC	3 cut(s)	1377, 1403, 1815
SmiMI	CAYNNNNRTG	1 cut(s)	1319
SmlI	CTYRAG	1 cut(s)	299
SmoI	CTYRAG	1 cut(s)	299
Sse9I	AATT	3 cut(s)	822, 1505, 1646
SspDI	GGCGCC	3 cut(s)	635, 784, 796
StyD4I	CCNGG	4 cut(s)	321, 1219, 1245, 1257
StyI	CCWWGG	2 cut(s)	201, 1325
TaaI	ACNGT	7 cut(s)	580, 598, 622, 646, 1525, 1540, 1564
TaiI	ACGT	4 cut(s)	264, 855, 1047, 1739
TaqI	TCGA	7 cut(s)	951, 1014, 1161, 1184, 1566, 1776, 1790
TaqII	GACCGA	2 cut(s)	122, 740
TasI	AATT	3 cut(s)	822, 1505, 1646
TatI	WGTACW	2 cut(s)	313, 810
TauI	GCSGC	5 cut(s)	88, 122, 361, 395, 785
Tru1I	TTAA	1 cut(s)	333
Tru9I	TTAA	1 cut(s)	333
TscAI	CASTG	4 cut(s)	355, 989, 1528, 1534
TseFI	GTSAC	5 cut(s)	103, 163, 586, 1249, 1525
Tsp45I	GTSAC	5 cut(s)	103, 163, 586, 1249, 1525
TspDTI	ATGAA	4 cut(s)	1105, 1256, 1303, 1518
TspGWI	ACGGA	3 cut(s)	476, 605, 1106
TspRI	CASTG	4 cut(s)	355, 989, 1528, 1534
Tth111I	GACNNNGTC	1 cut(s)	1523
Van91I	CCANNNNNTGG	1 cut(s)	1138
VpaK11BI	GGWCC	3 cut(s)	1377, 1403, 1815
XapI	RAATTY	3 cut(s)	822, 1505, 1646
XceI	RCATGY	1 cut(s)	1454
XmiI	GTMKAC	1 cut(s)	1014
ZrmI	AGTACT	1 cut(s)	315

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.