Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0008588

Physical Location & Seq

Genomic Coordinates Reverse (-)

28289218 .. 28296897

Gene Structure 7680 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1220100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1359 bp

ATGAGCACCGCCAACTCCAATAACAGTACCAACCACCACCACGGCGACCGAGATCTCGGCTTTTGGTTCCTCGAGCAATCGCGGCTCGCCTCTGCTCGGGGGCTGACCGACATGGACGAGGACGAGGACGCCCCCACGGAGCTCAACACCATCAACAGCTCCGGCGGCTTCCTCGTCGTGTCCCCTGACAAGCTCTCCGCCAAGTACCCCAACGTCAATCTTCACGGCCACGACGTCGGCGTAGTTCAGGCTAACAAGCCGGCTCCGGTCAAGCGGCTCGTCTACTACTTCGAGATTTATGTCAAGGATGCTGGGACCAAGGGCCATATTGCCATTGGATTTACTTCTGAGAGCTTCAAGATGCGGAGGCAGCCCGGATGGGAGGCAAATAGTTGTGGCTATCATGGCGATGATGGACTACTTTATCGGGGACATGGAAAAGGAGAAGCGTTTGGCCCAACCTACACCTCTGGGGATATAGTTGGAGGTGGTATCAATTATGATTCACAGGAATTCTTTTTCACTAAAAATGGTGCTGTGGTGGGAACAGTGCCCAAGGATATCAAGGGCCCCTTATTTCCTACCATTGCTGTACACAGCCAAAATGAGGAGTATGTGGAATTTGAAGCGCAAGAGAGGATGAAACAACAAATGTCAATTGAAAAAATTTCTTTGCCGACTAATGTTAGTCATCAAATTGTTCGCTCCTACTTACTACATTATGGATATGAAGATACGCTCAATTCATTTGACATGGCTAGTAAAAGTACTGTTCCTCCAGTACATATAGCTCAAGAAAATGGTTTTGATGAGCAGGACATTCTGTTTGCACTAAATGAGAGAAAGACTCTTAGACAGCTCATTAGGAATGGGGAGATCGACTTTGCTCTTGGTAAACTCCGTGAATGGTATCCCCAAATTGTTCAGGATGATAAATCTGCTACATGCTTTCTGCTCCACTGTCAAAAGTTTATTGAATTGGTTCGGGTCGGAGCACTGGAGGAGGCTGTCAAATATGGGAGGATGGAATTGGCAAGGTTCTTCGACTTGCCGCTGTTTGAGGACCTAGTTCAGGACTGTGTAACACTGCTGGCGTACGAAAGGCCACAGGAATCATCGGTTGGATATCTGCTTGAAGAGTCTCAGCGTGAAGTTGTAGCAGACACAGTTAATGCAATGATCTTGTCAACAAATCCCAACCTGAAAGATTCTTCGCACGGTTGCTTGCATTCATATCTGGAGAGGTTACTCAGGCAGCTTACTGCTTGCTGTTTGGAGAGAAGGTTTTTGAGCGGGGATCAAGGCGAAGCCTTCCATCTGCAGAGAGTACTTAACAGTAGTAAGAAAGCTAGGTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CLTH SPRY

Protein Analysis

453

Amino Acids

50.81

Weight (kDa)

5.55

Isoelectric Point (pI)

43.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPRY	PF00622	95 - 207	2.6e-20	SPRY domain
CTLH	PF10607	279 - 403	1.7e-20	CTLH/CRA C-terminal to LisH motif domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008588)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G35470 AT1G35470 AT4G09340
fragaria_vesca	FvH4_5g32270
malus_domestica	MD08G1220100.v1.1 MD15G1410300.v1.1
prunus_persica	Prupe.1G552200_v2.0.a1
pyrus_communis	pycom08g19100 pycom15g36520
rosa_chinensis	RchiOBHm_Chr7g0228461 RchiOBHm_Chr7g0228471
rosa_laevigata	RLG00000001364 RLG00000001561
rosa_multiflora	Rmu_sc0004041.1_g000005
rosa_roxburghii	Rroxscaffold_3G00231120
rosa_rugosa	Rorug07G0249700.1 Rorug07G0249800
rosa_samantha	Rh7AG402000 Rh7BG383000 Rh7BG399200 Rh7CG421000 Rh7DG398100
rosa_wichuraiana	Rw7G033420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	237
AccBSI	CCGCTC	1 cut(s)	1293
AccI	GTMKAC	1 cut(s)	282
AccII	CGCG	1 cut(s)	82
AciI	CCGC	8 cut(s)	9, 82, 165, 198, 274, 364, 1052, 1293
AclWI	GGATC	1 cut(s)	1305
AcoI	YGGCCR	1 cut(s)	226
AcsI	RAATTY	3 cut(s)	512, 620, 666
AcyI	GRCGYC	2 cut(s)	129, 234
AfaI	GTAC	7 cut(s)	28, 206, 594, 769, 783, 1097, 1329
AfiI	CCNNNNNNNGG	3 cut(s)	96, 607, 1072
AgsI	TTSAA	5 cut(s)	358, 626, 662, 977, 1136
AjuI	GAANNNNNNNTTGG	2 cut(s)	1104, 1136
AluBI	AGCT	8 cut(s)	142, 159, 193, 354, 791, 859, 1258, 1349
AluI	AGCT	8 cut(s)	142, 159, 193, 354, 791, 859, 1258, 1349
Alw21I	GWGCWC	3 cut(s)	8, 144, 997
Alw26I	GTCTC	1 cut(s)	1146
AlwI	GGATC	1 cut(s)	1305
Ama87I	CYCGRG	2 cut(s)	71, 96
AoxI	GGCC	5 cut(s)	226, 322, 454, 568, 1103
ApaI	GGGCCC	1 cut(s)	572
ApeKI	GCWGC	2 cut(s)	370, 1255
ApoI	RAATTY	3 cut(s)	512, 620, 666
ArsI	GACNNNNNNTTYG	2 cut(s)	809, 841
Asp700I	GAANNNNTTC	1 cut(s)	981
AspLEI	GCGC	1 cut(s)	631
AspS9I	GGNCC	6 cut(s)	315, 322, 455, 568, 569, 1063
AsuC2I	CCSGG	1 cut(s)	375
AvaI	CYCGRG	2 cut(s)	71, 96
AvaII	GGWCC	2 cut(s)	315, 1063
BaeGI	GKGCMC	2 cut(s)	555, 572
BanII	GRGCYC	2 cut(s)	144, 572
Bbv12I	GWGCWC	3 cut(s)	8, 144, 997
BbvI	GCAGC	2 cut(s)	382, 1267
BccI	CCATC	5 cut(s)	158, 372, 407, 1018, 1323
BceAI	ACGGC	2 cut(s)	58, 241
BciVI	GTATCC	1 cut(s)	921
BcnI	CCSGG	1 cut(s)	375
BcoDI	GTCTC	1 cut(s)	1146
BfaI	CTAG	4 cut(s)	759, 1067, 1350, 1357
BfmI	CTRYAG	1 cut(s)	1319
BfuI	GTATCC	1 cut(s)	921
BglII	AGATCT	1 cut(s)	52
BisI	GCNGC	6 cut(s)	83, 166, 275, 371, 1052, 1256
BlsI	GCNGC	6 cut(s)	84, 167, 276, 372, 1053, 1257
BmcAI	AGTACT	2 cut(s)	769, 1329
Bme1390I	CCNGG	1 cut(s)	375
Bme18I	GGWCC	2 cut(s)	315, 1063
BmeT110I	CYCGRG	2 cut(s)	71, 96
BmgT120I	GGNCC	6 cut(s)	315, 322, 455, 568, 569, 1063
BmiI	GGNNCC	5 cut(s)	68, 264, 316, 570, 571
BmrFI	CCNGG	1 cut(s)	375
BmsI	GCATC	2 cut(s)	298, 351
BplI	GAGNNNNNCTC	2 cut(s)	832, 864
BpmI	CTGGAG	3 cut(s)	762, 1019, 1259
BpuEI	CTTGAG	1 cut(s)	777
BpuMI	CCSGG	1 cut(s)	375
BsaHI	GRCGYC	2 cut(s)	129, 234
BsaJI	CCNNGG	4 cut(s)	40, 135, 318, 555
BsaWI	WCCGGW	1 cut(s)	265
BsaXI	ACNNNNNCTCC	6 cut(s)	179, 209, 760, 790, 1268, 1298
Bsc4I	CCNNNNNNNGG	3 cut(s)	96, 607, 1072
Bse118I	RCCGGY	1 cut(s)	259
Bse1I	ACTGG	2 cut(s)	779, 1002
Bse3DI	GCAATG	2 cut(s)	585, 1182
BseDI	CCNNGG	4 cut(s)	40, 135, 318, 555
BseGI	GGATG	5 cut(s)	313, 383, 645, 934, 1029
BseLI	CCNNNNNNNGG	3 cut(s)	96, 607, 1072
BseMI	GCAATG	2 cut(s)	585, 1182
BseMII	CTCAG	3 cut(s)	339, 1157, 1264
BseNI	ACTGG	2 cut(s)	779, 1002
BseRI	GAGGAG	2 cut(s)	623, 1016
BseSI	GKGCMC	2 cut(s)	555, 572
BseXI	GCAGC	2 cut(s)	382, 1267
BseYI	CCCAGC	1 cut(s)	311
Bsh1236I	CGCG	1 cut(s)	82
Bsh1285I	CGRYCG	1 cut(s)	49
BshFI	GGCC	5 cut(s)	228, 324, 456, 570, 1105
BsiEI	CGRYCG	1 cut(s)	49
BsiHKAI	GWGCWC	3 cut(s)	8, 144, 997
BsiHKCI	CYCGRG	2 cut(s)	71, 96
BsiSI	CCGG	4 cut(s)	162, 260, 266, 375
BsiWI	CGTACG	1 cut(s)	1095
BslFI	GGGAC	3 cut(s)	166, 328, 444
BslI	CCNNNNNNNGG	3 cut(s)	96, 607, 1072
BsmAI	GTCTC	1 cut(s)	1146
BsmFI	GGGAC	3 cut(s)	166, 328, 444
BsmI	GAATGC	1 cut(s)	1228
BsnI	GGCC	5 cut(s)	228, 324, 456, 570, 1105
BsoBI	CYCGRG	2 cut(s)	71, 96
Bsp120I	GGGCCC	1 cut(s)	568
Bsp1286I	GDGCHC	5 cut(s)	8, 144, 555, 572, 997
Bsp1407I	TGTACA	1 cut(s)	592
Bsp143I	GATC	4 cut(s)	52, 876, 1179, 1297
BspACI	CCGC	8 cut(s)	9, 82, 165, 198, 274, 364, 1052, 1293
BspANI	GGCC	5 cut(s)	228, 324, 456, 570, 1105
BspCNI	CTCAG	3 cut(s)	340, 1156, 1263
BspFNI	CGCG	1 cut(s)	82
BspLI	GGNNCC	5 cut(s)	68, 264, 316, 570, 571
BspMAI	CTGCAG	1 cut(s)	1323
BspPI	GGATC	1 cut(s)	1305
BsrBI	CCGCTC	1 cut(s)	1293
BsrDI	GCAATG	2 cut(s)	585, 1182
BsrFI	RCCGGY	1 cut(s)	259
BsrGI	TGTACA	1 cut(s)	592
BsrI	ACTGG	2 cut(s)	779, 1002
BssAI	RCCGGY	1 cut(s)	259
BssECI	CCNNGG	4 cut(s)	40, 135, 318, 555
BssMI	GATC	4 cut(s)	52, 876, 1179, 1297
BssNI	GRCGYC	2 cut(s)	129, 234
BssT1I	CCWWGG	2 cut(s)	318, 555
Bst4CI	ACNGT	8 cut(s)	26, 550, 772, 962, 1079, 1168, 1220, 1337
Bst6I	CTCTTC	1 cut(s)	1131
BstACI	GRCGYC	2 cut(s)	129, 234
BstAUI	TGTACA	1 cut(s)	592
BstC8I	GCNNGC	5 cut(s)	87, 261, 1092, 1226, 1267
BstDEI	CTNAG	4 cut(s)	348, 851, 1143, 1250
BstDSI	CCRYGG	2 cut(s)	40, 135
BstENI	CCTNNNNNAGG	1 cut(s)	1070
BstF5I	GGATG	5 cut(s)	313, 383, 645, 934, 1029
BstFNI	CGCG	1 cut(s)	82
BstHHI	GCGC	1 cut(s)	631
BstKTI	GATC	4 cut(s)	55, 879, 1182, 1300
BstMAI	GTCTC	1 cut(s)	1146
BstMBI	GATC	4 cut(s)	52, 876, 1179, 1297
BstMCI	CGRYCG	1 cut(s)	49
BstMWI	GCNNNNNNNGC	4 cut(s)	82, 165, 370, 405
BstNSI	RCATGY	1 cut(s)	948
BstSCI	CCNGG	1 cut(s)	373
BstSFI	CTRYAG	1 cut(s)	1319
BstSLI	GKGCMC	2 cut(s)	555, 572
BstUI	CGCG	1 cut(s)	82
BstV1I	GCAGC	2 cut(s)	382, 1267
BstX2I	RGATCY	1 cut(s)	52
BstYI	RGATCY	1 cut(s)	52
BsuI	GTATCC	1 cut(s)	921
BsuRI	GGCC	5 cut(s)	228, 324, 456, 570, 1105
BtgI	CCRYGG	2 cut(s)	40, 135
BtgZI	GCGATG	1 cut(s)	423
BtsCI	GGATG	5 cut(s)	313, 383, 645, 934, 1029
BtsI	GCAGTG	1 cut(s)	1085
BtsIMutI	CAGTG	4 cut(s)	555, 958, 995, 1085
Cac8I	GCNNGC	5 cut(s)	87, 261, 1092, 1226, 1267
CfoI	GCGC	1 cut(s)	631
Cfr10I	RCCGGY	1 cut(s)	259
Cfr13I	GGNCC	6 cut(s)	315, 322, 455, 568, 569, 1063
CseI	GACGC	1 cut(s)	137
Csp6I	GTAC	7 cut(s)	27, 205, 593, 768, 782, 1096, 1328
CviAII	CATG	5 cut(s)	112, 404, 434, 754, 945
CviQI	GTAC	7 cut(s)	27, 205, 593, 768, 782, 1096, 1328
DdeI	CTNAG	4 cut(s)	348, 851, 1143, 1250
DpnI	GATC	4 cut(s)	54, 878, 1181, 1299
DpnII	GATC	4 cut(s)	52, 876, 1179, 1297
EaeI	YGGCCR	1 cut(s)	226
Eam1104I	CTCTTC	1 cut(s)	1131
EarI	CTCTTC	1 cut(s)	1131
EciI	GGCGGA	1 cut(s)	187
Ecl136II	GAGCTC	1 cut(s)	142
Eco130I	CCWWGG	2 cut(s)	318, 555
Eco24I	GRGCYC	2 cut(s)	144, 572
Eco32I	GATATC	2 cut(s)	562, 1127
Eco47I	GGWCC	2 cut(s)	315, 1063
Eco53kI	GAGCTC	1 cut(s)	142
Eco88I	CYCGRG	2 cut(s)	71, 96
EcoICRI	GAGCTC	1 cut(s)	142
EcoNI	CCTNNNNNAGG	1 cut(s)	1070
EcoO109I	RGGNCCY	3 cut(s)	568, 569, 1063
EcoRI	GAATTC	1 cut(s)	512
EcoRV	GATATC	2 cut(s)	562, 1127
EcoT14I	CCWWGG	2 cut(s)	318, 555
EcoT38I	GRGCYC	2 cut(s)	144, 572
ErhI	CCWWGG	2 cut(s)	318, 555
FaeI	CATG	5 cut(s)	115, 407, 437, 757, 948
FalI	AAGNNNNNCTT	2 cut(s)	557, 589
FaqI	GGGAC	3 cut(s)	166, 328, 444
FatI	CATG	5 cut(s)	111, 403, 433, 753, 944
FauI	CCCGC	1 cut(s)	1286
FblI	GTMKAC	1 cut(s)	282
Fnu4HI	GCNGC	6 cut(s)	83, 166, 275, 371, 1052, 1256
FokI	GGATG	5 cut(s)	320, 390, 652, 941, 1036
FriOI	GRGCYC	2 cut(s)	144, 572
Fsp4HI	GCNGC	6 cut(s)	83, 166, 275, 371, 1052, 1256
FspBI	CTAG	4 cut(s)	759, 1067, 1350, 1357
GlaI	GCGC	1 cut(s)	630
GluI	GCNGC	6 cut(s)	83, 166, 275, 371, 1052, 1256
GsaI	CCCAGC	1 cut(s)	315
GsuI	CTGGAG	3 cut(s)	762, 1019, 1259
HaeIII	GGCC	5 cut(s)	228, 324, 456, 570, 1105
HapII	CCGG	4 cut(s)	162, 260, 266, 375
HgaI	GACGC	1 cut(s)	137
HhaI	GCGC	1 cut(s)	631
Hin1I	GRCGYC	2 cut(s)	129, 234
Hin1II	CATG	5 cut(s)	115, 407, 437, 757, 948
Hin6I	GCGC	1 cut(s)	629
HinP1I	GCGC	1 cut(s)	629
HincII	GTYRAC	1 cut(s)	1188
HindII	GTYRAC	1 cut(s)	1188
HinfI	GANTC	5 cut(s)	503, 847, 1112, 1139, 1208
HpaII	CCGG	4 cut(s)	162, 260, 266, 375
Hpy166II	GTNNAC	4 cut(s)	283, 595, 896, 1188
Hpy188I	TCNGA	2 cut(s)	349, 992
Hpy188III	TCNNGA	6 cut(s)	292, 358, 794, 926, 1073, 1238
Hpy8I	GTNNAC	4 cut(s)	283, 595, 896, 1188
Hpy99I	CGWCG	3 cut(s)	179, 236, 239
HpyAV	CCTTC	2 cut(s)	1275, 1321
HpyCH4III	ACNGT	8 cut(s)	26, 550, 772, 962, 1079, 1168, 1220, 1337
HpyCH4IV	ACGT	2 cut(s)	213, 234
HpyCH4V	TGCA	4 cut(s)	830, 1175, 1228, 1321
HpyF10VI	GCNNNNNNNGC	4 cut(s)	82, 165, 370, 405
HpyF3I	CTNAG	4 cut(s)	348, 851, 1143, 1250
HpySE526I	ACGT	2 cut(s)	213, 234
Hsp92I	GRCGYC	2 cut(s)	129, 234
Hsp92II	CATG	5 cut(s)	115, 407, 437, 757, 948
HspAI	GCGC	1 cut(s)	629
KroI	GCCGGC	1 cut(s)	259
KroNI	GCCGGC	1 cut(s)	261
Kzo9I	GATC	4 cut(s)	52, 876, 1179, 1297
LmnI	GCTCC	6 cut(s)	139, 164, 268, 710, 960, 992
Lsp1109I	GCAGC	2 cut(s)	382, 1267
LweI	GCATC	2 cut(s)	298, 351
MaeI	CTAG	4 cut(s)	759, 1067, 1350, 1357
MaeII	ACGT	2 cut(s)	213, 234
MaeIII	GTNAC	2 cut(s)	1081, 1245
MalI	GATC	4 cut(s)	54, 878, 1181, 1299
MbiI	CCGCTC	1 cut(s)	1293
MboI	GATC	4 cut(s)	52, 876, 1179, 1297
MboII	GAAGA	5 cut(s)	212, 743, 1033, 1148, 1203
MfeI	CAATTG	1 cut(s)	657
MflI	RGATCY	1 cut(s)	52
MhlI	GDGCHC	5 cut(s)	8, 144, 555, 572, 997
MlyI	GAGTC	2 cut(s)	841, 1148
MmeI	TCCRAC	3 cut(s)	463, 970, 1102
MroNI	GCCGGC	1 cut(s)	259
MroXI	GAANNNNTTC	1 cut(s)	981
MseI	TTAA	2 cut(s)	1170, 1332
MslI	CAYNNNNRTG	1 cut(s)	408
MspA1I	CMGCKG	1 cut(s)	1054
MspI	CCGG	4 cut(s)	162, 260, 266, 375
MspR9I	CCNGG	1 cut(s)	375
MunI	CAATTG	1 cut(s)	657
Mva1269I	GAATGC	1 cut(s)	1228
MvnI	CGCG	1 cut(s)	82
MwoI	GCNNNNNNNGC	4 cut(s)	82, 165, 370, 405
NaeI	GCCGGC	1 cut(s)	261
NciI	CCSGG	1 cut(s)	375
NdeII	GATC	4 cut(s)	52, 876, 1179, 1297
NgoMIV	GCCGGC	1 cut(s)	259
NlaIII	CATG	5 cut(s)	115, 407, 437, 757, 948
NlaIV	GGNNCC	5 cut(s)	68, 264, 316, 570, 571
NmeAIII	GCCGAG	1 cut(s)	36
NspI	RCATGY	1 cut(s)	948
PaeR7I	CTCGAG	1 cut(s)	71
PcsI	WCGNNNNNNNCGW	2 cut(s)	231, 237
PctI	GAATGC	1 cut(s)	1228
PdiI	GCCGGC	1 cut(s)	261
PdmI	GAANNNNTTC	1 cut(s)	981
PfeI	GAWTC	3 cut(s)	503, 1112, 1208
Pfl23II	CGTACG	1 cut(s)	1095
PkrI	GCNGC	6 cut(s)	84, 167, 276, 372, 1053, 1257
PleI	GAGTC	2 cut(s)	841, 1147
PpsI	GAGTC	2 cut(s)	841, 1147
PpuMI	RGGWCCY	1 cut(s)	1063
Psp124BI	GAGCTC	1 cut(s)	144
Psp5II	RGGWCCY	1 cut(s)	1063
PspFI	CCCAGC	1 cut(s)	311
PspLI	CGTACG	1 cut(s)	1095
PspN4I	GGNNCC	5 cut(s)	68, 264, 316, 570, 571
PspOMI	GGGCCC	1 cut(s)	568
PspPI	GGNCC	6 cut(s)	315, 322, 455, 568, 569, 1063
PspPPI	RGGWCCY	1 cut(s)	1063
PspXI	VCTCGAGB	1 cut(s)	71
PstI	CTGCAG	1 cut(s)	1323
PsuI	RGATCY	1 cut(s)	52
RsaI	GTAC	7 cut(s)	28, 206, 594, 769, 783, 1097, 1329
RsaNI	GTAC	7 cut(s)	27, 205, 593, 768, 782, 1096, 1328
RseI	CAYNNNNRTG	1 cut(s)	408
SacI	GAGCTC	1 cut(s)	144
SaqAI	TTAA	2 cut(s)	1170, 1332
SatI	GCNGC	6 cut(s)	83, 166, 275, 371, 1052, 1256
Sau3AI	GATC	4 cut(s)	52, 876, 1179, 1297
Sau96I	GGNCC	6 cut(s)	315, 322, 455, 568, 569, 1063
ScaI	AGTACT	2 cut(s)	769, 1329
SchI	GAGTC	2 cut(s)	841, 1148
ScrFI	CCNGG	1 cut(s)	375
SduI	GDGCHC	5 cut(s)	8, 144, 555, 572, 997
SfaNI	GCATC	2 cut(s)	298, 351
SfcI	CTRYAG	1 cut(s)	1319
Sfr274I	CTCGAG	1 cut(s)	71
SinI	GGWCC	2 cut(s)	315, 1063
SlaI	CTCGAG	1 cut(s)	71
SmiMI	CAYNNNNRTG	1 cut(s)	408
SmlI	CTYRAG	2 cut(s)	71, 792
SmoI	CTYRAG	2 cut(s)	71, 792
SsiI	CCGC	8 cut(s)	9, 82, 165, 198, 274, 364, 1052, 1293
SspMI	CTAG	4 cut(s)	759, 1067, 1350, 1357
SstI	GAGCTC	1 cut(s)	144
StyD4I	CCNGG	1 cut(s)	373
StyI	CCWWGG	2 cut(s)	318, 555
TaaI	ACNGT	8 cut(s)	26, 550, 772, 962, 1079, 1168, 1220, 1337
TaiI	ACGT	2 cut(s)	216, 237
TaqI	TCGA	4 cut(s)	72, 291, 879, 1044
TaqII	GACCGA	2 cut(s)	63, 122
TatI	WGTACW	4 cut(s)	592, 767, 781, 1327
TauI	GCSGC	4 cut(s)	85, 168, 277, 1054
TfiI	GAWTC	3 cut(s)	503, 1112, 1208
Tru1I	TTAA	2 cut(s)	1170, 1332
Tru9I	TTAA	2 cut(s)	1170, 1332
TscAI	CASTG	4 cut(s)	555, 965, 1002, 1092
TseI	GCWGC	2 cut(s)	370, 1255
TspDTI	ATGAA	4 cut(s)	656, 735, 744, 1221
TspGWI	ACGGA	2 cut(s)	152, 890
TspRI	CASTG	4 cut(s)	555, 965, 1002, 1092
VpaK11BI	GGWCC	2 cut(s)	315, 1063
XagI	CCTNNNNNAGG	1 cut(s)	1070
XapI	RAATTY	3 cut(s)	512, 620, 666
XceI	RCATGY	1 cut(s)	948
XcmI	CCANNNNNNNNNTGG	1 cut(s)	332
XhoI	CTCGAG	1 cut(s)	71
XmiI	GTMKAC	1 cut(s)	282
XmnI	GAANNNNTTC	1 cut(s)	981
XspI	CTAG	4 cut(s)	759, 1067, 1350, 1357
ZraI	GACGTC	1 cut(s)	235
ZrmI	AGTACT	2 cut(s)	769, 1329

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.