RchiOBHm_Chr7g0228471

Ran-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0008588
Physical Location & Seq
Forward (+)
51811213 .. 51817378
6166 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20467

Sequence Viewer

Length: 978 bp
ATGATGATCACTGGTATTGTTGCTGTTGCTGTTACAATATCTTTTGCTTATCTGCGTGCGCTAGCTGTCATCGTAAGGTTTCAACACTCAAAGACCGATGCTTTTGTTCAACTCTCAATCAACCGTCTTCAAAAATGTACATGCCACTTTTGTCCCGATCTACAAGTAAATAAAGCAACTTTTGAGTCCTATTCATGGACAAAGCAAACAAGTGGAATAAGAAAGAGAACAGAATTTGAAGCACAAGAGAGGATGAAGCAGCAAATGATGATTGAAAAGATTTCGTTGCCAACTAATGTTAGTCATGGAATTGTGCGCTCCTACTTGCTACATTATGGCTATGAAGATACGCTCAATTCATTTGACATGGCTAGTAAAAGTACTGTTCCCCCTGTATATATAGCTCAAGAAAATGGCTTTGATGAGCAGGATATTTTGTATGCACTAAATCAAAGAAACACTCTTAGACAGCTTGTCAGGAATGGAGAGATAGACTCTGCACTCGTTAAACTCCGTGAATGGTATCCCCAAATTGTTCAGGATGATAAATCAGCTACTTGCTTTCTGCTTCACTGTCAGAAGTTTATTGAATTGGTTCGGGTTGGATCATTGGAGGAGGCAGTGAAATATGGAAGGATTGAATTGGCAAAGTTTTTTGGCTTGCCGGTGTTTAAGGAAATAGTTCAGGATTGTGTTGCTCTGCTGGCATACGTACGCCCACGGGAGTCCTCGGTTGGATATCTGCTTGAAGAGTCGCAACGTGAAGTTGTGGCAGACACAGTCAATGCAATGATCTTGTCGACGAATCCTAACCTGAAAGATTCAAATAGTTTCTTGCATTCTTATCTTGAGAGGTTACTTAGGCAGCTCACGGCTTGCAGTTTGGAGCGAAGGTCCTTGAGTGGGGATCAAGGTGAAGCATTCCATTTGCACAGGGTACTTAACAGTTGTAAGAAGGCTAGATTAGGTGCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLTH SPRY

Protein Analysis

325

Amino Acids

37.08

Weight (kDa)

8.54

Isoelectric Point (pI)

41.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CTLH PF10607 150 - 284 3.2e-21 CTLH/CRA C-terminal to LisH motif domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 800
AclWI GGATC 2 cut(s) 613, 915
AcsI RAATTY 1 cut(s) 233
AfaI GTAC 4 cut(s) 139, 382, 714, 939
AfiI CCNNNNNNNGG 2 cut(s) 195, 903
AgsI TTSAA 9 cut(s) 83, 110, 131, 239, 275, 590, 641, 749, 825
AhdI GACNNNNNGTC 1 cut(s) 473
AluBI AGCT 6 cut(s) 65, 404, 472, 554, 868, 975
AluI AGCT 6 cut(s) 65, 404, 472, 554, 868, 975
AlwI GGATC 2 cut(s) 613, 915
ApeKI GCWGC 2 cut(s) 259, 865
ApoI RAATTY 1 cut(s) 233
Asp700I GAANNNNTTC 2 cut(s) 594, 681
AspLEI GCGC 2 cut(s) 61, 318
AspS9I GGNCC 1 cut(s) 894
AsuHPI GGTGA 1 cut(s) 926
AsuNHI GCTAGC 2 cut(s) 61, 971
AvaII GGWCC 1 cut(s) 894
BbsI GAAGAC 1 cut(s) 119
BbvI GCAGC 2 cut(s) 271, 877
BceAI ACGGC 1 cut(s) 888
BciVI GTATCC 1 cut(s) 534
BclI TGATCA 1 cut(s) 6
BfaI CTAG 5 cut(s) 62, 372, 960, 972, 976
BfuI GTATCC 1 cut(s) 534
BisI GCNGC 2 cut(s) 260, 866
BlsI GCNGC 2 cut(s) 261, 867
BmcAI AGTACT 1 cut(s) 382
Bme18I GGWCC 1 cut(s) 894
BmeRI GACNNNNNGTC 1 cut(s) 473
BmgT120I GGNCC 1 cut(s) 894
BmsI GCATC 1 cut(s) 88
BmtI GCTAGC 2 cut(s) 65, 975
BpiI GAAGAC 1 cut(s) 119
BplI GAGNNNNNCTC 2 cut(s) 479, 511
BpuEI CTTGAG 3 cut(s) 390, 869, 919
BsaAI YACGTR 1 cut(s) 712
BsaJI CCNNGG 2 cut(s) 719, 729
BsaXI ACNNNNNCTCC 2 cut(s) 878, 908
Bsc4I CCNNNNNNNGG 2 cut(s) 195, 903
Bse118I RCCGGY 1 cut(s) 664
Bse1I ACTGG 1 cut(s) 16
Bse3DI GCAATG 1 cut(s) 795
BseDI CCNNGG 2 cut(s) 719, 729
BseGI GGATG 2 cut(s) 258, 547
BseLI CCNNNNNNNGG 2 cut(s) 195, 903
BseMI GCAATG 1 cut(s) 795
BseNI ACTGG 1 cut(s) 16
BseRI GAGGAG 1 cut(s) 629
BseXI GCAGC 2 cut(s) 271, 877
BsgI GTGCAG 1 cut(s) 483
BsiSI CCGG 1 cut(s) 665
BsiWI CGTACG 1 cut(s) 712
BslFI GGGAC 1 cut(s) 138
BslI CCNNNNNNNGG 2 cut(s) 195, 903
BsmFI GGGAC 1 cut(s) 138
BsmI GAATGC 2 cut(s) 838, 920
Bsp1407I TGTACA 1 cut(s) 137
Bsp143I GATC 5 cut(s) 6, 157, 605, 792, 907
BspOI GCTAGC 2 cut(s) 65, 975
BspPI GGATC 2 cut(s) 613, 915
BsrDI GCAATG 1 cut(s) 795
BsrFI RCCGGY 1 cut(s) 664
BsrGI TGTACA 1 cut(s) 137
BsrI ACTGG 1 cut(s) 16
BssAI RCCGGY 1 cut(s) 664
BssECI CCNNGG 2 cut(s) 719, 729
BssMI GATC 5 cut(s) 6, 157, 605, 792, 907
Bst4CI ACNGT 5 cut(s) 125, 385, 575, 781, 947
Bst6I CTCTTC 1 cut(s) 744
BstAUI TGTACA 1 cut(s) 137
BstBAI YACGTR 1 cut(s) 712
BstC8I GCNNGC 6 cut(s) 57, 63, 662, 705, 877, 973
BstDEI CTNAG 2 cut(s) 464, 860
BstDSI CCRYGG 1 cut(s) 719
BstF5I GGATG 2 cut(s) 258, 547
BstHHI GCGC 2 cut(s) 61, 318
BstKTI GATC 5 cut(s) 9, 160, 608, 795, 910
BstMBI GATC 5 cut(s) 6, 157, 605, 792, 907
BstMWI GCNNNNNNNGC 1 cut(s) 704
BstNSI RCATGY 1 cut(s) 144
BstSNI TACGTA 1 cut(s) 712
BstV1I GCAGC 2 cut(s) 271, 877
BstV2I GAAGAC 1 cut(s) 119
BsuI GTATCC 1 cut(s) 534
BtgI CCRYGG 1 cut(s) 719
BtsCI GGATG 2 cut(s) 258, 547
BtsI GCAGTG 1 cut(s) 627
BtsIMutI CAGTG 3 cut(s) 9, 571, 627
Cac8I GCNNGC 6 cut(s) 57, 63, 662, 705, 877, 973
CfoI GCGC 2 cut(s) 61, 318
Cfr10I RCCGGY 1 cut(s) 664
Cfr13I GGNCC 1 cut(s) 894
Csp6I GTAC 4 cut(s) 138, 381, 713, 938
CviAII CATG 4 cut(s) 141, 195, 305, 367
CviQI GTAC 4 cut(s) 138, 381, 713, 938
DdeI CTNAG 2 cut(s) 464, 860
DpnI GATC 5 cut(s) 8, 159, 607, 794, 909
DpnII GATC 5 cut(s) 6, 157, 605, 792, 907
DriI GACNNNNNGTC 1 cut(s) 473
Eam1104I CTCTTC 1 cut(s) 744
Eam1105I GACNNNNNGTC 1 cut(s) 473
EarI CTCTTC 1 cut(s) 744
Eco105I TACGTA 1 cut(s) 712
Eco32I GATATC 1 cut(s) 740
Eco47I GGWCC 1 cut(s) 894
EcoO109I RGGNCCY 1 cut(s) 894
EcoRV GATATC 1 cut(s) 740
FaeI CATG 4 cut(s) 144, 198, 308, 370
FaqI GGGAC 1 cut(s) 138
FatI CATG 4 cut(s) 140, 194, 304, 366
FbaI TGATCA 1 cut(s) 6
FblI GTMKAC 1 cut(s) 800
Fnu4HI GCNGC 2 cut(s) 260, 866
FokI GGATG 2 cut(s) 265, 554
Fsp4HI GCNGC 2 cut(s) 260, 866
FspBI CTAG 5 cut(s) 62, 372, 960, 972, 976
GlaI GCGC 2 cut(s) 60, 317
GluI GCNGC 2 cut(s) 260, 866
HapII CCGG 1 cut(s) 665
HhaI GCGC 2 cut(s) 61, 318
Hin1II CATG 4 cut(s) 144, 198, 308, 370
Hin6I GCGC 2 cut(s) 59, 316
HinP1I GCGC 2 cut(s) 59, 316
HincII GTYRAC 1 cut(s) 801
HindII GTYRAC 1 cut(s) 801
HinfI GANTC 6 cut(s) 185, 494, 725, 752, 805, 821
HpaII CCGG 1 cut(s) 665
HphI GGTGA 1 cut(s) 926
Hpy166II GTNNAC 1 cut(s) 801
Hpy188I TCNGA 1 cut(s) 579
Hpy188III TCNNGA 6 cut(s) 155, 407, 478, 539, 686, 848
Hpy8I GTNNAC 1 cut(s) 801
Hpy99I CGWCG 1 cut(s) 805
HpyAV CCTTC 3 cut(s) 627, 885, 949
HpyCH4III ACNGT 5 cut(s) 125, 385, 575, 781, 947
HpyCH4IV ACGT 2 cut(s) 711, 760
HpyCH4V TGCA 6 cut(s) 443, 500, 788, 838, 879, 931
HpyF10VI GCNNNNNNNGC 1 cut(s) 704
HpyF3I CTNAG 2 cut(s) 464, 860
HpySE526I ACGT 2 cut(s) 711, 760
Hsp92II CATG 4 cut(s) 144, 198, 308, 370
HspAI GCGC 2 cut(s) 59, 316
Ksp22I TGATCA 1 cut(s) 6
Kzo9I GATC 5 cut(s) 6, 157, 605, 792, 907
LmnI GCTCC 2 cut(s) 323, 886
LpnPI CCDG 9 cut(s) 405, 413, 463, 524, 671, 678, 689, 827, 919
Lsp1109I GCAGC 2 cut(s) 271, 877
LweI GCATC 1 cut(s) 88
MaeI CTAG 5 cut(s) 62, 372, 960, 972, 976
MaeII ACGT 2 cut(s) 711, 760
MaeIII GTNAC 2 cut(s) 31, 855
MalI GATC 5 cut(s) 8, 159, 607, 794, 909
MboI GATC 5 cut(s) 6, 157, 605, 792, 907
MboII GAAGA 3 cut(s) 119, 356, 761
MluCI AATT 6 cut(s) 233, 309, 355, 531, 590, 641
MlyI GAGTC 4 cut(s) 194, 488, 734, 761
MmeI TCCRAC 2 cut(s) 583, 715
MnlI CCTC 5 cut(s) 243, 607, 610, 739, 846
MroXI GAANNNNTTC 2 cut(s) 594, 681
MseI TTAA 3 cut(s) 507, 672, 942
MspI CCGG 1 cut(s) 665
Mva1269I GAATGC 2 cut(s) 838, 920
MwoI GCNNNNNNNGC 1 cut(s) 704
NdeII GATC 5 cut(s) 6, 157, 605, 792, 907
NheI GCTAGC 2 cut(s) 61, 971
NlaIII CATG 4 cut(s) 144, 198, 308, 370
NspI RCATGY 1 cut(s) 144
PctI GAATGC 2 cut(s) 838, 920
PdmI GAANNNNTTC 2 cut(s) 594, 681
PfeI GAWTC 2 cut(s) 805, 821
Pfl23II CGTACG 1 cut(s) 712
PflFI GACNNNGTC 1 cut(s) 779
PkrI GCNGC 2 cut(s) 261, 867
PleI GAGTC 4 cut(s) 193, 488, 733, 760
PpsI GAGTC 4 cut(s) 193, 488, 733, 760
Ppu21I YACGTR 1 cut(s) 712
PpuMI RGGWCCY 1 cut(s) 894
Psp5II RGGWCCY 1 cut(s) 894
PspLI CGTACG 1 cut(s) 712
PspPI GGNCC 1 cut(s) 894
PspPPI RGGWCCY 1 cut(s) 894
PsyI GACNNNGTC 1 cut(s) 779
RsaI GTAC 4 cut(s) 139, 382, 714, 939
RsaNI GTAC 4 cut(s) 138, 381, 713, 938
SalI GTCGAC 1 cut(s) 799
SaqAI TTAA 3 cut(s) 507, 672, 942
SatI GCNGC 2 cut(s) 260, 866
Sau3AI GATC 5 cut(s) 6, 157, 605, 792, 907
Sau96I GGNCC 1 cut(s) 894
ScaI AGTACT 1 cut(s) 382
SchI GAGTC 4 cut(s) 194, 488, 734, 761
SfaNI GCATC 1 cut(s) 88
SinI GGWCC 1 cut(s) 894
SmlI CTYRAG 3 cut(s) 405, 848, 898
SmoI CTYRAG 3 cut(s) 405, 848, 898
SnaBI TACGTA 1 cut(s) 712
Sse9I AATT 6 cut(s) 233, 309, 355, 531, 590, 641
SspMI CTAG 5 cut(s) 62, 372, 960, 972, 976
TaaI ACNGT 5 cut(s) 125, 385, 575, 781, 947
TaiI ACGT 2 cut(s) 714, 763
TaqI TCGA 1 cut(s) 800
TaqII GACCGA 1 cut(s) 110
TasI AATT 6 cut(s) 233, 309, 355, 531, 590, 641
TatI WGTACW 2 cut(s) 137, 380
TfiI GAWTC 2 cut(s) 805, 821
Tru1I TTAA 3 cut(s) 507, 672, 942
Tru9I TTAA 3 cut(s) 507, 672, 942
TscAI CASTG 3 cut(s) 16, 578, 627
TseI GCWGC 2 cut(s) 259, 865
TspDTI ATGAA 4 cut(s) 183, 269, 348, 357
TspGWI ACGGA 1 cut(s) 503
TspRI CASTG 3 cut(s) 16, 578, 627
Tth111I GACNNNGTC 1 cut(s) 779
VpaK11BI GGWCC 1 cut(s) 894
XapI RAATTY 1 cut(s) 233
XceI RCATGY 1 cut(s) 144
XmiI GTMKAC 1 cut(s) 800
XmnI GAANNNNTTC 2 cut(s) 594, 681
XspI CTAG 5 cut(s) 62, 372, 960, 972, 976
ZrmI AGTACT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.