pycom08g19100

Ran-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008588
Physical Location & Seq
Reverse (-)
19146028 .. 19153128
7101 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g19100.1

Sequence Viewer

Length: 1392 bp
ATGAGCACCGCCAACTCCAATAACAGTACCAACCACCACCACGGCGACCGAGATCTCAGCTTGTGGTTCCTCGAGCAACCGCGGCTCGCCTCTGCTCGGGGGCTGACCGACGAGGACGAGGACTCCCCCACGGAGCTCAACACCATCAACAGCTCCGGCGGCTTCATCGTCGTGTCCCCTGATAAGCTCTCCGCCAAGTACACCAGCGTCAATCTTCACGGCCACGACGTCGGCGTAGTTCAGGCTAACAAGCCAGCTCCGGTCAAGCGGCTCGTCTACTACTTCGAGATTTATGTCAAGGATGCTGGGACCAAGGGCCAGATTTCCATTGGATTTACTTCTGAGAGCTTCAAGATGCGGAGGCAGCCCGGATGGGAGGCAAATAGTTGTGGCTATCATGGCGATGATGGACTACTTTATCGGGGACATGGAAAAGGAGAAGCGTTTGGCCCAACCTACACCTCTGGGGATATAGTTGGAGGTGGTATCAATTATGATTCACAGGAATTCTTTTTCACTAAAAATGGTGCTGTGGTGGGAACAGTGCCCAAGGATATCAAGGGCCCCTTATTTCCTACCATTGCTGTACACAGCCAAAATGAGGAGGTACACGTTAACTTTGGACAGCAACCATTTGCGTTTGATCTTAAGGAATTTGAAGCGCAAGAGAGGATGAAACAACAAATGTCAATTGAAAAAATTTCTTTGCCGACTAATGTTAGTCATCAAATTGTTCGCTCCTACTTACTACATTATGGATATGAAGATACGCTCAATTCATTTGACATGGCTAGTAAAAGTACTGTTCCTCCAGTACATATAGCTCAAGAAAATGGTTTTGATGAGCAGGACATTCTGTTTGCACTAAATGAGAGAAAGGCTCTTAGACAGCTCATTAGGAATGGGGAGATCGACTCTGCTCTTGGTAAACTCCGTGAATGGTATCCCCAAATTGTTCAGGATGATAAATCTGCTACGTGCTTTCTGCTCCACTGTCAAAAGTTTATTGAATTGGTTCGGGTCGGAGCACTGGAGGAGGCTGTCAAATATGGGAGGATGGAATTGGCAAAGTTCTTTGGCTTGCCGCTGTTTGAGGACCTAGTTCAGGACTGTGTTGCACTGCTGGCGTACGAAAGGCCACGGGAATCATCGGTTGGATATCTGCTTGAAGAGTCTCAGCGTGAAGTTGTAGCAGACACAGTTAATGCAATGATCCTGTCAACAAATCCCAACCTGAAAGATTCTTCGCACGGTGGCTTGCATTCATATCTGGAGAGGTTACTCAGGCAGCTTACTGCTTGCTGTTTGGAGAGAAGGTTTTTGAGTGGGGATCAAGGCGAAGCCTTCCATCTGCAGAGAGTACTTAACAGTAGTAAGAAAGCTAGGTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLTH SPRY

Protein Analysis

464

Amino Acids

51.81

Weight (kDa)

5.76

Isoelectric Point (pI)

41.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPRY PF00622 93 - 210 5.3e-22 SPRY domain
CTLH PF10607 290 - 414 6.9e-21 CTLH/CRA C-terminal to LisH motif domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 231
AccI GTMKAC 1 cut(s) 276
AccII CGCG 1 cut(s) 82
AciI CCGC 8 cut(s) 9, 80, 82, 159, 192, 268, 358, 1085
AclWI GGATC 2 cut(s) 1207, 1338
AcoI YGGCCR 1 cut(s) 220
AcsI RAATTY 3 cut(s) 506, 653, 699
AcyI GRCGYC 1 cut(s) 228
AfaI GTAC 8 cut(s) 28, 200, 588, 609, 802, 816, 1130, 1362
AfiI CCNNNNNNNGG 3 cut(s) 96, 601, 1105
AflII CTTAAG 1 cut(s) 647
AflIII ACRYGT 1 cut(s) 610
AgsI TTSAA 5 cut(s) 352, 659, 695, 1010, 1169
AjuI GAANNNNNNNTTGG 2 cut(s) 1137, 1169
Alw21I GWGCWC 3 cut(s) 8, 138, 1030
Alw26I GTCTC 1 cut(s) 1179
AlwI GGATC 2 cut(s) 1207, 1338
Ama87I CYCGRG 2 cut(s) 71, 96
AoxI GGCC 5 cut(s) 220, 316, 448, 562, 1136
ApaI GGGCCC 1 cut(s) 566
ApeKI GCWGC 2 cut(s) 364, 1288
ApoI RAATTY 3 cut(s) 506, 653, 699
ArsI GACNNNNNNTTYG 2 cut(s) 842, 874
Asp700I GAANNNNTTC 1 cut(s) 1014
AspLEI GCGC 1 cut(s) 664
AspS9I GGNCC 6 cut(s) 309, 316, 449, 562, 563, 1096
AsuC2I CCSGG 1 cut(s) 369
AvaI CYCGRG 2 cut(s) 71, 96
AvaII GGWCC 2 cut(s) 309, 1096
BaeGI GKGCMC 2 cut(s) 549, 566
BanII GRGCYC 2 cut(s) 138, 566
Bbv12I GWGCWC 3 cut(s) 8, 138, 1030
BbvI GCAGC 2 cut(s) 376, 1300
BccI CCATC 5 cut(s) 152, 366, 401, 1051, 1356
BceAI ACGGC 2 cut(s) 58, 235
BciVI GTATCC 1 cut(s) 954
BcnI CCSGG 1 cut(s) 369
BcoDI GTCTC 1 cut(s) 1179
BfaI CTAG 4 cut(s) 792, 1100, 1383, 1390
BfmI CTRYAG 1 cut(s) 1352
BfrI CTTAAG 1 cut(s) 647
BfuI GTATCC 1 cut(s) 954
BglII AGATCT 1 cut(s) 52
BisI GCNGC 6 cut(s) 83, 160, 269, 365, 1085, 1289
BlsI GCNGC 6 cut(s) 84, 161, 270, 366, 1086, 1290
BmcAI AGTACT 2 cut(s) 802, 1362
Bme1390I CCNGG 1 cut(s) 369
Bme18I GGWCC 2 cut(s) 309, 1096
BmeT110I CYCGRG 2 cut(s) 71, 96
BmgT120I GGNCC 6 cut(s) 309, 316, 449, 562, 563, 1096
BmiI GGNNCC 4 cut(s) 68, 310, 564, 565
BmrFI CCNGG 1 cut(s) 369
BmsI GCATC 2 cut(s) 292, 345
BplI GAGNNNNNCTC 4 cut(s) 865, 897, 899, 931
BpmI CTGGAG 3 cut(s) 795, 1052, 1292
BpuEI CTTGAG 1 cut(s) 810
BpuMI CCSGG 1 cut(s) 369
BsaAI YACGTR 1 cut(s) 978
BsaHI GRCGYC 1 cut(s) 228
BsaJI CCNNGG 6 cut(s) 40, 80, 129, 312, 549, 1139
BsaWI WCCGGW 1 cut(s) 259
BsaXI ACNNNNNCTCC 6 cut(s) 107, 137, 793, 823, 1301, 1331
Bsc4I CCNNNNNNNGG 3 cut(s) 96, 601, 1105
Bse1I ACTGG 2 cut(s) 812, 1035
Bse3DI GCAATG 2 cut(s) 579, 1215
BseDI CCNNGG 6 cut(s) 40, 80, 129, 312, 549, 1139
BseGI GGATG 5 cut(s) 307, 377, 678, 967, 1062
BseLI CCNNNNNNNGG 3 cut(s) 96, 601, 1105
BseMI GCAATG 2 cut(s) 579, 1215
BseMII CTCAG 4 cut(s) 70, 333, 1190, 1297
BseNI ACTGG 2 cut(s) 812, 1035
BseRI GAGGAG 2 cut(s) 617, 1049
BseSI GKGCMC 2 cut(s) 549, 566
BseXI GCAGC 2 cut(s) 376, 1300
BseYI CCCAGC 1 cut(s) 305
Bsh1236I CGCG 1 cut(s) 82
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 5 cut(s) 222, 318, 450, 564, 1138
BsiEI CGRYCG 1 cut(s) 49
BsiHKAI GWGCWC 3 cut(s) 8, 138, 1030
BsiHKCI CYCGRG 2 cut(s) 71, 96
BsiSI CCGG 3 cut(s) 156, 260, 369
BsiWI CGTACG 1 cut(s) 1128
BslFI GGGAC 3 cut(s) 160, 322, 438
BslI CCNNNNNNNGG 3 cut(s) 96, 601, 1105
BsmAI GTCTC 1 cut(s) 1179
BsmFI GGGAC 3 cut(s) 160, 322, 438
BsmI GAATGC 1 cut(s) 1261
BsnI GGCC 5 cut(s) 222, 318, 450, 564, 1138
BsoBI CYCGRG 2 cut(s) 71, 96
Bsp120I GGGCCC 1 cut(s) 562
Bsp1286I GDGCHC 5 cut(s) 8, 138, 549, 566, 1030
Bsp1407I TGTACA 1 cut(s) 586
Bsp143I GATC 5 cut(s) 52, 643, 909, 1212, 1330
BspACI CCGC 8 cut(s) 9, 80, 82, 159, 192, 268, 358, 1085
BspANI GGCC 5 cut(s) 222, 318, 450, 564, 1138
BspCNI CTCAG 4 cut(s) 69, 334, 1189, 1296
BspFNI CGCG 1 cut(s) 82
BspLI GGNNCC 4 cut(s) 68, 310, 564, 565
BspMAI CTGCAG 1 cut(s) 1356
BspPI GGATC 2 cut(s) 1207, 1338
BspTI CTTAAG 1 cut(s) 647
BsrDI GCAATG 2 cut(s) 579, 1215
BsrGI TGTACA 1 cut(s) 586
BsrI ACTGG 2 cut(s) 812, 1035
BssECI CCNNGG 6 cut(s) 40, 80, 129, 312, 549, 1139
BssMI GATC 5 cut(s) 52, 643, 909, 1212, 1330
BssNI GRCGYC 1 cut(s) 228
BssT1I CCWWGG 2 cut(s) 312, 549
Bst4CI ACNGT 8 cut(s) 26, 544, 805, 995, 1112, 1201, 1253, 1370
Bst6I CTCTTC 1 cut(s) 1164
BstACI GRCGYC 1 cut(s) 228
BstAFI CTTAAG 1 cut(s) 647
BstAUI TGTACA 1 cut(s) 586
BstBAI YACGTR 1 cut(s) 978
BstC8I GCNNGC 6 cut(s) 87, 255, 1082, 1125, 1259, 1300
BstDEI CTNAG 5 cut(s) 56, 342, 884, 1176, 1283
BstDSI CCRYGG 4 cut(s) 40, 80, 129, 1139
BstENI CCTNNNNNAGG 1 cut(s) 1103
BstF5I GGATG 5 cut(s) 307, 377, 678, 967, 1062
BstFNI CGCG 1 cut(s) 82
BstHHI GCGC 1 cut(s) 664
BstKTI GATC 5 cut(s) 55, 646, 912, 1215, 1333
BstMAI GTCTC 1 cut(s) 1179
BstMBI GATC 5 cut(s) 52, 643, 909, 1212, 1330
BstMCI CGRYCG 1 cut(s) 49
BstMWI GCNNNNNNNGC 5 cut(s) 82, 159, 364, 399, 1124
BstSCI CCNGG 1 cut(s) 367
BstSFI CTRYAG 1 cut(s) 1352
BstSLI GKGCMC 2 cut(s) 549, 566
BstUI CGCG 1 cut(s) 82
BstV1I GCAGC 2 cut(s) 376, 1300
BstX2I RGATCY 1 cut(s) 52
BstYI RGATCY 1 cut(s) 52
BsuI GTATCC 1 cut(s) 954
BsuRI GGCC 5 cut(s) 222, 318, 450, 564, 1138
BtgI CCRYGG 4 cut(s) 40, 80, 129, 1139
BtgZI GCGATG 1 cut(s) 417
BtsCI GGATG 5 cut(s) 307, 377, 678, 967, 1062
BtsI GCAGTG 1 cut(s) 1118
BtsIMutI CAGTG 4 cut(s) 549, 991, 1028, 1118
Cac8I GCNNGC 6 cut(s) 87, 255, 1082, 1125, 1259, 1300
CfoI GCGC 1 cut(s) 664
Cfr13I GGNCC 6 cut(s) 309, 316, 449, 562, 563, 1096
Cfr42I CCGCGG 1 cut(s) 83
CseI GACGC 1 cut(s) 196
Csp6I GTAC 8 cut(s) 27, 199, 587, 608, 801, 815, 1129, 1361
CviAII CATG 3 cut(s) 398, 428, 787
CviQI GTAC 8 cut(s) 27, 199, 587, 608, 801, 815, 1129, 1361
DdeI CTNAG 5 cut(s) 56, 342, 884, 1176, 1283
DpnI GATC 5 cut(s) 54, 645, 911, 1214, 1332
DpnII GATC 5 cut(s) 52, 643, 909, 1212, 1330
EaeI YGGCCR 1 cut(s) 220
Eam1104I CTCTTC 1 cut(s) 1164
EarI CTCTTC 1 cut(s) 1164
EciI GGCGGA 1 cut(s) 181
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 2 cut(s) 312, 549
Eco24I GRGCYC 2 cut(s) 138, 566
Eco32I GATATC 2 cut(s) 556, 1160
Eco47I GGWCC 2 cut(s) 309, 1096
Eco53kI GAGCTC 1 cut(s) 136
Eco88I CYCGRG 2 cut(s) 71, 96
EcoICRI GAGCTC 1 cut(s) 136
EcoNI CCTNNNNNAGG 1 cut(s) 1103
EcoO109I RGGNCCY 3 cut(s) 562, 563, 1096
EcoRI GAATTC 1 cut(s) 506
EcoRV GATATC 2 cut(s) 556, 1160
EcoT14I CCWWGG 2 cut(s) 312, 549
EcoT38I GRGCYC 2 cut(s) 138, 566
ErhI CCWWGG 2 cut(s) 312, 549
FaeI CATG 3 cut(s) 401, 431, 790
FalI AAGNNNNNCTT 2 cut(s) 551, 583
FaqI GGGAC 3 cut(s) 160, 322, 438
FatI CATG 3 cut(s) 397, 427, 786
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 6 cut(s) 83, 160, 269, 365, 1085, 1289
FokI GGATG 5 cut(s) 314, 384, 685, 974, 1069
FriOI GRGCYC 2 cut(s) 138, 566
Fsp4HI GCNGC 6 cut(s) 83, 160, 269, 365, 1085, 1289
FspBI CTAG 4 cut(s) 792, 1100, 1383, 1390
GlaI GCGC 1 cut(s) 663
GluI GCNGC 6 cut(s) 83, 160, 269, 365, 1085, 1289
GsaI CCCAGC 1 cut(s) 309
GsuI CTGGAG 3 cut(s) 795, 1052, 1292
HaeIII GGCC 5 cut(s) 222, 318, 450, 564, 1138
HapII CCGG 3 cut(s) 156, 260, 369
HgaI GACGC 1 cut(s) 196
HhaI GCGC 1 cut(s) 664
Hin1I GRCGYC 1 cut(s) 228
Hin1II CATG 3 cut(s) 401, 431, 790
Hin6I GCGC 1 cut(s) 662
HinP1I GCGC 1 cut(s) 662
HincII GTYRAC 2 cut(s) 616, 1221
HindII GTYRAC 2 cut(s) 616, 1221
HinfI GANTC 6 cut(s) 122, 497, 914, 1145, 1172, 1241
HpaI GTTAAC 1 cut(s) 616
HpaII CCGG 3 cut(s) 156, 260, 369
Hpy166II GTNNAC 7 cut(s) 201, 277, 589, 610, 616, 929, 1221
Hpy188I TCNGA 2 cut(s) 343, 1025
Hpy188III TCNNGA 6 cut(s) 286, 352, 827, 959, 1106, 1271
Hpy8I GTNNAC 7 cut(s) 201, 277, 589, 610, 616, 929, 1221
Hpy99I CGWCG 4 cut(s) 113, 173, 230, 233
HpyAV CCTTC 2 cut(s) 1308, 1354
HpyCH4III ACNGT 8 cut(s) 26, 544, 805, 995, 1112, 1201, 1253, 1370
HpyCH4IV ACGT 3 cut(s) 228, 612, 977
HpyCH4V TGCA 5 cut(s) 863, 1118, 1208, 1261, 1354
HpyF10VI GCNNNNNNNGC 5 cut(s) 82, 159, 364, 399, 1124
HpyF3I CTNAG 5 cut(s) 56, 342, 884, 1176, 1283
HpySE526I ACGT 3 cut(s) 228, 612, 977
Hsp92I GRCGYC 1 cut(s) 228
Hsp92II CATG 3 cut(s) 401, 431, 790
HspAI GCGC 1 cut(s) 662
KspAI GTTAAC 1 cut(s) 616
KspI CCGCGG 1 cut(s) 83
Kzo9I GATC 5 cut(s) 52, 643, 909, 1212, 1330
LmnI GCTCC 6 cut(s) 133, 158, 262, 743, 993, 1025
Lsp1109I GCAGC 2 cut(s) 376, 1300
LweI GCATC 2 cut(s) 292, 345
MaeI CTAG 4 cut(s) 792, 1100, 1383, 1390
MaeII ACGT 3 cut(s) 228, 612, 977
MaeIII GTNAC 1 cut(s) 1278
MalI GATC 5 cut(s) 54, 645, 911, 1214, 1332
MboI GATC 5 cut(s) 52, 643, 909, 1212, 1330
MboII GAAGA 4 cut(s) 206, 776, 1181, 1236
MfeI CAATTG 1 cut(s) 690
MflI RGATCY 1 cut(s) 52
MhlI GDGCHC 5 cut(s) 8, 138, 549, 566, 1030
MlyI GAGTC 3 cut(s) 116, 908, 1181
MmeI TCCRAC 3 cut(s) 457, 1003, 1135
MroXI GAANNNNTTC 1 cut(s) 1014
MseI TTAA 4 cut(s) 615, 648, 1203, 1365
MslI CAYNNNNRTG 2 cut(s) 170, 402
MspA1I CMGCKG 2 cut(s) 82, 1087
MspCI CTTAAG 1 cut(s) 647
MspI CCGG 3 cut(s) 156, 260, 369
MspR9I CCNGG 1 cut(s) 369
MunI CAATTG 1 cut(s) 690
Mva1269I GAATGC 1 cut(s) 1261
MvnI CGCG 1 cut(s) 82
MwoI GCNNNNNNNGC 5 cut(s) 82, 159, 364, 399, 1124
NciI CCSGG 1 cut(s) 369
NdeII GATC 5 cut(s) 52, 643, 909, 1212, 1330
NlaIII CATG 3 cut(s) 401, 431, 790
NlaIV GGNNCC 4 cut(s) 68, 310, 564, 565
PaeR7I CTCGAG 1 cut(s) 71
PcsI WCGNNNNNNNCGW 2 cut(s) 225, 231
PctI GAATGC 1 cut(s) 1261
PdmI GAANNNNTTC 1 cut(s) 1014
PfeI GAWTC 3 cut(s) 497, 1145, 1241
Pfl23II CGTACG 1 cut(s) 1128
PkrI GCNGC 6 cut(s) 84, 161, 270, 366, 1086, 1290
PleI GAGTC 3 cut(s) 116, 908, 1180
PpsI GAGTC 3 cut(s) 116, 908, 1180
Ppu21I YACGTR 1 cut(s) 978
PpuMI RGGWCCY 1 cut(s) 1096
Psp124BI GAGCTC 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 1096
PspFI CCCAGC 1 cut(s) 305
PspLI CGTACG 1 cut(s) 1128
PspN4I GGNNCC 4 cut(s) 68, 310, 564, 565
PspOMI GGGCCC 1 cut(s) 562
PspPI GGNCC 6 cut(s) 309, 316, 449, 562, 563, 1096
PspPPI RGGWCCY 1 cut(s) 1096
PspXI VCTCGAGB 1 cut(s) 71
PstI CTGCAG 1 cut(s) 1356
PsuI RGATCY 1 cut(s) 52
RsaI GTAC 8 cut(s) 28, 200, 588, 609, 802, 816, 1130, 1362
RsaNI GTAC 8 cut(s) 27, 199, 587, 608, 801, 815, 1129, 1361
RseI CAYNNNNRTG 2 cut(s) 170, 402
SacI GAGCTC 1 cut(s) 138
SacII CCGCGG 1 cut(s) 83
SaqAI TTAA 4 cut(s) 615, 648, 1203, 1365
SatI GCNGC 6 cut(s) 83, 160, 269, 365, 1085, 1289
Sau3AI GATC 5 cut(s) 52, 643, 909, 1212, 1330
Sau96I GGNCC 6 cut(s) 309, 316, 449, 562, 563, 1096
ScaI AGTACT 2 cut(s) 802, 1362
SchI GAGTC 3 cut(s) 116, 908, 1181
ScrFI CCNGG 1 cut(s) 369
SduI GDGCHC 5 cut(s) 8, 138, 549, 566, 1030
SfaNI GCATC 2 cut(s) 292, 345
SfcI CTRYAG 1 cut(s) 1352
Sfr274I CTCGAG 1 cut(s) 71
Sfr303I CCGCGG 1 cut(s) 83
SgrBI CCGCGG 1 cut(s) 83
SinI GGWCC 2 cut(s) 309, 1096
SlaI CTCGAG 1 cut(s) 71
SmiMI CAYNNNNRTG 2 cut(s) 170, 402
SmlI CTYRAG 3 cut(s) 71, 647, 825
SmoI CTYRAG 3 cut(s) 71, 647, 825
SsiI CCGC 8 cut(s) 9, 80, 82, 159, 192, 268, 358, 1085
SspMI CTAG 4 cut(s) 792, 1100, 1383, 1390
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 1 cut(s) 367
StyI CCWWGG 2 cut(s) 312, 549
TaaI ACNGT 8 cut(s) 26, 544, 805, 995, 1112, 1201, 1253, 1370
TaiI ACGT 3 cut(s) 231, 615, 980
TaqI TCGA 3 cut(s) 72, 285, 912
TaqII GACCGA 2 cut(s) 63, 122
TatI WGTACW 5 cut(s) 198, 586, 800, 814, 1360
TauI GCSGC 4 cut(s) 85, 162, 271, 1087
TfiI GAWTC 3 cut(s) 497, 1145, 1241
Tru1I TTAA 4 cut(s) 615, 648, 1203, 1365
Tru9I TTAA 4 cut(s) 615, 648, 1203, 1365
TscAI CASTG 4 cut(s) 549, 998, 1035, 1125
TseI GCWGC 2 cut(s) 364, 1288
TspDTI ATGAA 5 cut(s) 154, 689, 768, 777, 1254
TspGWI ACGGA 2 cut(s) 146, 923
TspRI CASTG 4 cut(s) 549, 998, 1035, 1125
Vha464I CTTAAG 1 cut(s) 647
VpaK11BI GGWCC 2 cut(s) 309, 1096
XagI CCTNNNNNAGG 1 cut(s) 1103
XapI RAATTY 3 cut(s) 506, 653, 699
XcmI CCANNNNNNNNNTGG 1 cut(s) 326
XhoI CTCGAG 1 cut(s) 71
XmiI GTMKAC 1 cut(s) 276
XmnI GAANNNNTTC 1 cut(s) 1014
XspI CTAG 4 cut(s) 792, 1100, 1383, 1390
ZraI GACGTC 1 cut(s) 229
ZrmI AGTACT 2 cut(s) 802, 1362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.