Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0015638

Physical Location & Seq

Genomic Coordinates Forward (+)

28327919 .. 28329873

Gene Structure 1955 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1220500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 786 bp

ATGGGGAGAGCACCTTGTTGTGAGAAAGCTCACACCAACAAAGGGGCATGGTCGAAAGAGGAGGACCAGCGCCTCATCGACTACATCCGCCAACACGGCGAGGGTTGCTGGCGCTCCCTCCCTAAAGCCGCCGGGTTGCTTAGGTGCGGCAAGAGTTGCAGATTGAGATGGATAAACTACCTCCGCCCCGACCTCAAGCGTGGGAATTTCACACAGGAAGAAGATGAACTCATCATCAAGCTTCATAGCTTGCTGGGAAACAAATGGTCGTTGATTGCGGCGCGATTGCCGGGAAGAACCGATAACGAGATTAAAAACTACTGGAACACTCACGTCAAGCGAAATCTCATCAGTCGCGGCCTCGACCCTCAAACCCACCGCCCGCTTAACCAAGCAACAACCGCCACTTCCACTGCTCCCGCCTCTCGCTTGGGCTTGAGAAACCGTCCTCCGCCGTCTTCCGTGTTGTTTGATCATAAACCCGTCAACAACCACAGCAAAATTGAATTGTTGAAGCACCCCAAGATGGAGCAGGACTACTACAATTACAAAATAGAATCGGAGGCCAATTGCTCCACCGCTACCGGCAGCGGCACCACAACCGAAGAAGACCAACAACAACAACAGCAGAAATACAAGTGTGGTGATCTCAACTTGGATCTTTCAATTGGGTTAGAGCCGTTTCAGTCCAAGCCAACTCGGGCGTCGTCTGGGAACTCGGCCGAGTCAAGACTACAGCAGATCATAGTTCCTCCTAATAGTAATAATAATCATCTGTTTTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006082 GO:0006355 GO:0006357 GO:0006725 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009611 GO:0009628 GO:0009651 GO:0009698 GO:0009699 GO:0009719 GO:0009725 GO:0009737 GO:0009739 GO:0009751 GO:0009753 GO:0009800 GO:0009803 GO:0009889 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0014070 GO:0016053 GO:0019219 GO:0019222 GO:0019438 GO:0019748 GO:0019752 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0032787 GO:0033993 GO:0042221 GO:0042493 GO:0042537 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0044212 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0044550 GO:0046394 GO:0046677 GO:0048519 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0071704 GO:0072330 GO:0080090 GO:0097159 GO:0097305 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-binding

Protein Analysis

262

Amino Acids

29.66

Weight (kDa)

9.31

Isoelectric Point (pI)

56.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	14 - 61	7.1e-17	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	17 - 76	2.9e-13	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	67 - 111	1.2e-19	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	70 - 115	7.6e-10	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015638)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g32320
malus_domestica	MD08G1220500.v1.1 MD15G1411200.v1.1
prunus_persica	Prupe.1G551400_v2.0.a1 Prupe.1G551400_v2.0.a1
pyrus_communis	pycom08g19130 pycom15g36600
rosa_chinensis	RchiOBHm_Chr7g0228621
rosa_laevigata	RLG00000001551
rosa_roxburghii	Rroxscaffold_3G00231000
rosa_rugosa	Rorug07G0251000.1
rosa_samantha	Rh7AG403100 Rh7BG383900 Rh7CG422400
rosa_wichuraiana	Rw7G033490 Rw7G033520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	593
AccII	CGCG	2 cut(s)	283, 357
AclWI	GGATC	1 cut(s)	666
AcoI	YGGCCR	1 cut(s)	720
AcsI	RAATTY	1 cut(s)	205
AcyI	GRCGYC	1 cut(s)	704
AfiI	CCNNNNNNNGG	2 cut(s)	42, 526
AgsI	TTSAA	3 cut(s)	506, 514, 666
AjiI	CACGTC	1 cut(s)	334
AluBI	AGCT	3 cut(s)	29, 241, 249
AluI	AGCT	3 cut(s)	29, 241, 249
Alw21I	GWGCWC	1 cut(s)	13
AlwI	GGATC	1 cut(s)	666
Ama87I	CYCGRG	1 cut(s)	699
AoxI	GGCC	3 cut(s)	358, 564, 720
ApeKI	GCWGC	1 cut(s)	588
ApoI	RAATTY	1 cut(s)	205
AspLEI	GCGC	3 cut(s)	72, 114, 283
AspS9I	GGNCC	1 cut(s)	64
AsuC2I	CCSGG	2 cut(s)	133, 291
AsuHPI	GGTGA	1 cut(s)	656
AvaI	CYCGRG	1 cut(s)	699
AvaII	GGWCC	1 cut(s)	64
BanI	GGYRCC	1 cut(s)	593
BbsI	GAAGAC	2 cut(s)	450, 615
Bbv12I	GWGCWC	1 cut(s)	13
BbvI	GCAGC	1 cut(s)	600
BccI	CCATC	2 cut(s)	162, 520
BceAI	ACGGC	3 cut(s)	112, 439, 664
BclI	TGATCA	1 cut(s)	472
BcnI	CCSGG	2 cut(s)	133, 291
BfmI	CTRYAG	1 cut(s)	734
BfoI	RGCGCY	2 cut(s)	73, 115
BglI	GCCNNNNNGGC	1 cut(s)	96
BisI	GCNGC	6 cut(s)	129, 148, 279, 358, 589, 592
BlsI	GCNGC	6 cut(s)	130, 149, 280, 359, 590, 593
Bme1390I	CCNGG	2 cut(s)	133, 291
Bme18I	GGWCC	1 cut(s)	64
BmeT110I	CYCGRG	1 cut(s)	699
BmgBI	CACGTC	1 cut(s)	334
BmgT120I	GGNCC	1 cut(s)	64
BmiI	GGNNCC	1 cut(s)	595
BmrFI	CCNGG	2 cut(s)	133, 291
BpiI	GAAGAC	2 cut(s)	450, 615
Bpu10I	CCTNAGC	1 cut(s)	140
BpuEI	CTTGAG	2 cut(s)	179, 457
BpuMI	CCSGG	2 cut(s)	133, 291
BsaHI	GRCGYC	1 cut(s)	704
Bsc4I	CCNNNNNNNGG	2 cut(s)	42, 526
Bse118I	RCCGGY	1 cut(s)	584
Bse1I	ACTGG	1 cut(s)	326
BseGI	GGATG	1 cut(s)	84
BseLI	CCNNNNNNNGG	2 cut(s)	42, 526
BseNI	ACTGG	1 cut(s)	326
BseRI	GAGGAG	1 cut(s)	74
BseX3I	CGGCCG	1 cut(s)	720
BseXI	GCAGC	1 cut(s)	600
BseYI	CCCAGC	1 cut(s)	253
Bsh1236I	CGCG	2 cut(s)	283, 357
Bsh1285I	CGRYCG	1 cut(s)	723
BshFI	GGCC	3 cut(s)	360, 566, 722
BshNI	GGYRCC	1 cut(s)	593
BsiEI	CGRYCG	1 cut(s)	723
BsiHKAI	GWGCWC	1 cut(s)	13
BsiHKCI	CYCGRG	1 cut(s)	699
BsiSI	CCGG	3 cut(s)	132, 290, 585
BslI	CCNNNNNNNGG	2 cut(s)	42, 526
BsnI	GGCC	3 cut(s)	360, 566, 722
BsoBI	CYCGRG	1 cut(s)	699
Bsp1286I	GDGCHC	1 cut(s)	13
Bsp143I	GATC	4 cut(s)	472, 646, 658, 741
BspANI	GGCC	3 cut(s)	360, 566, 722
BspFNI	CGCG	2 cut(s)	283, 357
BspLI	GGNNCC	1 cut(s)	595
BspPI	GGATC	1 cut(s)	666
BspT107I	GGYRCC	1 cut(s)	593
BsrFI	RCCGGY	1 cut(s)	584
BsrI	ACTGG	1 cut(s)	326
BssAI	RCCGGY	1 cut(s)	584
BssMI	GATC	4 cut(s)	472, 646, 658, 741
BssNI	GRCGYC	1 cut(s)	704
Bst4CI	ACNGT	1 cut(s)	446
BstACI	GRCGYC	1 cut(s)	704
BstAPI	GCANNNNNTGC	1 cut(s)	156
BstC8I	GCNNGC	3 cut(s)	110, 251, 383
BstDEI	CTNAG	1 cut(s)	140
BstF5I	GGATG	1 cut(s)	84
BstFNI	CGCG	2 cut(s)	283, 357
BstH2I	RGCGCY	2 cut(s)	73, 115
BstHHI	GCGC	3 cut(s)	72, 114, 283
BstKTI	GATC	4 cut(s)	475, 649, 661, 744
BstMBI	GATC	4 cut(s)	472, 646, 658, 741
BstMCI	CGRYCG	1 cut(s)	723
BstMWI	GCNNNNNNNGC	4 cut(s)	96, 105, 156, 401
BstSCI	CCNGG	2 cut(s)	131, 289
BstSFI	CTRYAG	1 cut(s)	734
BstUI	CGCG	2 cut(s)	283, 357
BstV1I	GCAGC	1 cut(s)	600
BstV2I	GAAGAC	2 cut(s)	450, 615
BstX2I	RGATCY	1 cut(s)	658
BstYI	RGATCY	1 cut(s)	658
BstZI	CGGCCG	1 cut(s)	720
BsuRI	GGCC	3 cut(s)	360, 566, 722
BtrI	CACGTC	1 cut(s)	334
BtsCI	GGATG	1 cut(s)	84
BtsI	GCAGTG	1 cut(s)	411
BtsIMutI	CAGTG	1 cut(s)	411
Cac8I	GCNNGC	3 cut(s)	110, 251, 383
CfoI	GCGC	3 cut(s)	72, 114, 283
Cfr10I	RCCGGY	1 cut(s)	584
Cfr13I	GGNCC	1 cut(s)	64
CseI	GACGC	1 cut(s)	693
CviAII	CATG	1 cut(s)	48
DdeI	CTNAG	1 cut(s)	140
DpnI	GATC	4 cut(s)	474, 648, 660, 743
DpnII	GATC	4 cut(s)	472, 646, 658, 741
EaeI	YGGCCR	1 cut(s)	720
EagI	CGGCCG	1 cut(s)	720
EciI	GGCGGA	3 cut(s)	77, 173, 441
EclXI	CGGCCG	1 cut(s)	720
Eco47I	GGWCC	1 cut(s)	64
Eco52I	CGGCCG	1 cut(s)	720
Eco88I	CYCGRG	1 cut(s)	699
FaeI	CATG	1 cut(s)	51
FaiI	YATR	4 cut(s)	49, 246, 477, 746
FatI	CATG	1 cut(s)	47
FauI	CCCGC	2 cut(s)	390, 427
FbaI	TGATCA	1 cut(s)	472
Fnu4HI	GCNGC	6 cut(s)	129, 148, 279, 358, 589, 592
FokI	GGATG	1 cut(s)	71
Fsp4HI	GCNGC	6 cut(s)	129, 148, 279, 358, 589, 592
GlaI	GCGC	3 cut(s)	71, 113, 282
GluI	GCNGC	6 cut(s)	129, 148, 279, 358, 589, 592
GsaI	CCCAGC	1 cut(s)	257
HaeII	RGCGCY	2 cut(s)	73, 115
HaeIII	GGCC	3 cut(s)	360, 566, 722
HapII	CCGG	3 cut(s)	132, 290, 585
HgaI	GACGC	1 cut(s)	693
HhaI	GCGC	3 cut(s)	72, 114, 283
Hin1I	GRCGYC	1 cut(s)	704
Hin1II	CATG	1 cut(s)	51
Hin6I	GCGC	3 cut(s)	70, 112, 281
HinP1I	GCGC	3 cut(s)	70, 112, 281
HincII	GTYRAC	1 cut(s)	487
HindII	GTYRAC	1 cut(s)	487
HindIII	AAGCTT	1 cut(s)	239
HinfI	GANTC	2 cut(s)	557, 725
HpaII	CCGG	3 cut(s)	132, 290, 585
HphI	GGTGA	1 cut(s)	656
Hpy166II	GTNNAC	1 cut(s)	487
Hpy188I	TCNGA	1 cut(s)	562
Hpy188III	TCNNGA	1 cut(s)	729
Hpy8I	GTNNAC	1 cut(s)	487
Hpy99I	CGWCG	1 cut(s)	709
HpyCH4III	ACNGT	1 cut(s)	446
HpyCH4IV	ACGT	1 cut(s)	333
HpyCH4V	TGCA	1 cut(s)	159
HpyF10VI	GCNNNNNNNGC	4 cut(s)	96, 105, 156, 401
HpyF3I	CTNAG	1 cut(s)	140
HpySE526I	ACGT	1 cut(s)	333
Hsp92I	GRCGYC	1 cut(s)	704
Hsp92II	CATG	1 cut(s)	51
HspAI	GCGC	3 cut(s)	70, 112, 281
Ksp22I	TGATCA	1 cut(s)	472
Kzo9I	GATC	4 cut(s)	472, 646, 658, 741
LmnI	GCTCC	4 cut(s)	119, 421, 529, 578
Lsp1109I	GCAGC	1 cut(s)	600
MaeII	ACGT	1 cut(s)	333
MalI	GATC	4 cut(s)	474, 648, 660, 743
MboI	GATC	4 cut(s)	472, 646, 658, 741
MboII	GAAGA	6 cut(s)	230, 233, 306, 450, 617, 620
MfeI	CAATTG	2 cut(s)	568, 666
MflI	RGATCY	1 cut(s)	658
MhlI	GDGCHC	1 cut(s)	13
MluCI	AATT	6 cut(s)	205, 501, 506, 544, 568, 666
MlyI	GAGTC	1 cut(s)	734
MseI	TTAA	2 cut(s)	312, 387
MspA1I	CMGCKG	1 cut(s)	591
MspI	CCGG	3 cut(s)	132, 290, 585
MspR9I	CCNGG	2 cut(s)	133, 291
MunI	CAATTG	2 cut(s)	568, 666
MvnI	CGCG	2 cut(s)	283, 357
MwoI	GCNNNNNNNGC	4 cut(s)	96, 105, 156, 401
NciI	CCSGG	2 cut(s)	133, 291
NdeII	GATC	4 cut(s)	472, 646, 658, 741
NlaIII	CATG	1 cut(s)	51
NlaIV	GGNNCC	1 cut(s)	595
NmeAIII	GCCGAG	2 cut(s)	698, 748
PfeI	GAWTC	1 cut(s)	557
PkrI	GCNGC	6 cut(s)	130, 149, 280, 359, 590, 593
PleI	GAGTC	1 cut(s)	733
PpsI	GAGTC	1 cut(s)	733
PspFI	CCCAGC	1 cut(s)	253
PspN4I	GGNNCC	1 cut(s)	595
PspPI	GGNCC	1 cut(s)	64
PsuI	RGATCY	1 cut(s)	658
SaqAI	TTAA	2 cut(s)	312, 387
SatI	GCNGC	6 cut(s)	129, 148, 279, 358, 589, 592
Sau3AI	GATC	4 cut(s)	472, 646, 658, 741
Sau96I	GGNCC	1 cut(s)	64
SchI	GAGTC	1 cut(s)	734
ScrFI	CCNGG	2 cut(s)	133, 291
SduI	GDGCHC	1 cut(s)	13
SetI	ASST	8 cut(s)	16, 31, 146, 183, 195, 243, 251, 336
SfcI	CTRYAG	1 cut(s)	734
SinI	GGWCC	1 cut(s)	64
SmlI	CTYRAG	2 cut(s)	194, 436
SmoI	CTYRAG	2 cut(s)	194, 436
Sse9I	AATT	6 cut(s)	205, 501, 506, 544, 568, 666
StyD4I	CCNGG	2 cut(s)	131, 289
TaaI	ACNGT	1 cut(s)	446
TaiI	ACGT	1 cut(s)	336
TaqI	TCGA	3 cut(s)	53, 78, 363
TasI	AATT	6 cut(s)	205, 501, 506, 544, 568, 666
TauI	GCSGC	5 cut(s)	131, 150, 281, 360, 594
TfiI	GAWTC	1 cut(s)	557
Tru1I	TTAA	2 cut(s)	312, 387
Tru9I	TTAA	2 cut(s)	312, 387
TscAI	CASTG	1 cut(s)	418
TseI	GCWGC	1 cut(s)	588
TspDTI	ATGAA	2 cut(s)	233, 240
TspGWI	ACGGA	1 cut(s)	451
TspRI	CASTG	1 cut(s)	418
VpaK11BI	GGWCC	1 cut(s)	64
XapI	RAATTY	1 cut(s)	205

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.