Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0005632

Physical Location & Seq

Genomic Coordinates Forward (+)

30997471 .. 31011169

Gene Structure 13699 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1245400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3006 bp

ATGATCAAAACATTCAGCATTATTCACCAACTCTATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTAATCTGCGGGAGTTTGCTGTCTCTGTGAACCCAGTTGGTTGGAGTTGCAGTCTGATAACTGTCGGTATGCTTGAGAAGAAGATATTCGAGAATGAGTTTGCTGAAAGAGATAACGAGCATTCCGATTCTTCACTTGAAAAGGTTGACCCTGGGAAGCCTGCATCACTGACATGGCAACGAAAATTAAACAGCACGGGCAACGCACCTTTGCCATTCAATCTATATTTAAAAGAGATCATTCATTTGGCTCCAATAGGTATTCGGCTATGGCGTCATCTACGAGAAGAAGCTGCTAAGGGAAGGGAGGGCTTCATTAATCCATTTGTTAAGCACCATTTGACATCTAATCATGGTGTCCCACTTGGTGGAATTGGGGCAGGAAGCATCGGAAGAAGTTATAGTGGTGATTTTCAGCGGTGGCAGCTCTTCCCTGAAAGATGTGAAGAGAAACCTGTTTTAGCAGATCAATTTTCTGTATTTGTTTCCCGCACGAATGGTGAAAAATACTCTACTGTGCTCTGTCCAAGGATCCCAGACGTTCTGAAAGAAAGTCAAGTTTCAGGGATTGGATCTTGGGACTGGAATCTGAACGGGGATAATTCTACATATCATGCCCTTTTTCCAAGGGCTTGGTCTGTTTACGACGGTGAACCTGATCCAGCTCTCAAAATAGTCTGTCGTCAAATTTCGCCTTTTATTCCCCATAATTACAAGGAGAGCAGCCTACCTGTTTCAGTTTTTACTTACACGATATATAATTCTGGGAAGACTGCAGCTGATGTCACACTGCTTTTCACTTGGGCAAATTCTGTTGGAGGGACTTCTGAACTTTCTGGTCATCACTCCAATTCAAGAGCAGTGATGAAGGATGGCGTGCATGGCGTACTTCTACATCACAAGACTGCAAATGGGCTGCCCCCAGTGACATTTGCAATAGCAGCACAGGAGACTGATGGTGTTCACGTATCAGAGTGCCCTTGCTTTGTGATTTCTGGCGACTCCAAAGGTATCACAGCTAAGAACATGTGGAGTGAGATAAAAGAGCATGGATCCTTTGACAGTCTTAATTCTGCAGAAACGCCATTACCTTCAGAACCCGGATCATCTATTGGGGCAGCCATTGCTGCGTCTGTGGCAGTTCCACCTGATGGAGTACGCACTGTAACATTTTCATTGGCATGGGACTGCCCAGAAGTAAAATTTATGGGTGGGAAGACGTATCACAGGCGGTACTCTAAGTTCTATGGCAAACTTGGGGATGCAGCTGCAAATATTGCTCATGATGCTATTCTTGAGCATCACCATTGGGAATCCCAGATTGAAGCATGGCAAAGACCAGTTCTTGAGGACAAGAGACTTCCTGAATGGTACCCTGTCACTCTGTTCAATGAGCTCTACATTCTGAATTCTGGGGGCACAGTTTGGACAGATGGAGCACCGCCAGTCCATAGCTTAACGAGCATTGGGGAAAGGAAGTTTTCCCTGGATAAGTCATCATTAGGTTTGAAAAGCGTCATTGACGCATCCCAGCAAAATGATACTGCTATTGACATTCTTGGAAGGATGACATCAATACTTGAGCAAGTTCACACTCCCATAGCATCAAACTCTGCGTTTGGAACAAATCTCCTTCAGGAGGGAGAAGAAAACATTGGCCAATTTCTCTGTCTTGAAGGAATTGAATATCATATGTGGAACAGTTACGATGTCCATTTCTACTCTTCGTTTGCACTAGTCATGCTATTCCCAAAACTTGAGCTCAGCATCCAACGGGACTTTGCAGCAGCAGTAATGATGCACGACCCCTCTAAGATGAGACTTCTATCTGATGGAAAATGGGTCCAAAGAAAGGTTCTTGGAGCTGTTCCTCATGATATCGGCCTCCATGACCCCTGGTTTGAAGTAAATGCGTATAACCTGTATAACACGGATAGGTGGAAAGACTTGAATCCTAAGTTTGTTCTCCAAGTCTACAGGGATGTGGTTGCCACTGGTGATAAGAAATTTGCAGAAGCCGTTTGGCCCGCTGTTTATGTTGCAATGGCTTATATGGAACAATTTGATAAGGATGGCGATGGAATGGTTGAGAGTGATGGCTCCCCAGATCAGACTTATGATACGTGGTCGGTCTCTGGTGTGGGTGCATATAGTGGTGGGCTGTGGGTAGCAGCATTGCAGGCTGCATCAGCCCTGGCACGAGAAGTAGGTGACAAGGGTTCAGAGGTTTATTTTTGGCACAAGTATCAGAAGGCAAAATCTGTGTATGCGAAATTATGGAATGGATCTTACTTTAATTATGACAACAGTGGTCAAACATCAAGTTCATCAATCCAAGCTGATCAACTGGCCGGACAATGGTATGCAAGAGCATCCGGTCTGTTGCCAATTGTTGATGAAGACAAGGCACGGAGTGCGCTGGAAATGGTTTATAATTACAATGTCCTAAAGTTTAAGGATGGGAGACAAGGAGCAGTTAATGGGATGCTACCCGATGGAAAGGTTGACATGTCATCAATGCAGTCAAGAGAAATATGGTCTGGAGTTACATACGCTGTAGCTGCAACGATGATTCAGGAAGATATGGTTGATATAGGATTTCATACTGCGGCTGGAGTCTATGAAGCTGCATGGTCCAAAGAAGGTCTTGGATACTCTTTTCAGACTCCCGAAGCTTGGACCACCAGCGGAGAATTCAGATCTTTAACTTACATGCGCCCTCTGGCCATTTGGTCAATGCAGTGGGCATTATCAAAACCACCGCTGTTCAAGCAGGAGACGGAGTTAGAAGCAGCTGAAGTTTCTTCGTTTAGGCACGAGGCTGGATTCTCGAAAGTTGCTCAGCTTCTAAAGTTGCCGGAAGACAATTCATCAAGAAGTATTTTGCAGACTGTTTTCGATTATACTTGCAAGAGGATGTGGTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004348 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005790 GO:0005886 GO:0006082 GO:0006629 GO:0006643 GO:0006664 GO:0006665 GO:0006687 GO:0006807 GO:0007275 GO:0007399 GO:0007417 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008422 GO:0009056 GO:0009987 GO:0012505 GO:0015926 GO:0016020 GO:0016021 GO:0016137 GO:0016139 GO:0016787 GO:0016798 GO:0019752 GO:0021953 GO:0021954 GO:0022008 GO:0030154 GO:0030182 GO:0031224 GO:0032501 GO:0032502 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0048468 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0071704 GO:0071944 GO:1901135 GO:1901136 GO:1901360 GO:1901564 GO:1901575 GO:1901615 GO:1901657 GO:1901658 GO:1903509

KEGG Pathways

Metabolic & Signaling

ko00511 ko00600 ko01100 map00511 map00600 map01100

Pfam Domains

Protein Families

DUF608 Glyco_hydr_116N

Protein Analysis

1002

Amino Acids

111.3

Weight (kDa)

5.72

Isoelectric Point (pI)

36.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydr_116N	PF12215	145 - 463	8e-112	beta-glucosidase 2, glycosyl-hydrolase family 116 N-term
DUF608	PF04685	579 - 939	6.4e-162	Glycosyl-hydrolase family 116, catalytic region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005632)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49900 AT5G49900 AT5G49900
fragaria_vesca	FvH4_5g39140 FvH4_5g39140 FvH4_5g39140 FvH4_5g39140 FvH4_5g39140
malus_domestica	MD08G1245400.v1.1 MD15G1440400.v1.1
prunus_persica	Prupe.1G582500_v2.0.a1 Prupe.1G582500_v2.0.a1
pyrus_communis	pycom08g21280 pycom15g38820
rosa_chinensis	RchiOBHm_Chr7g0241141
rosa_laevigata	RLG00000000592
rosa_multiflora	Rmu_sc0006665.1_g000004
rosa_roxburghii	Rroxscaffold_3G00219230
rosa_rugosa	Rorug07G0343100 Rorug07G0343200 Rorug07G0343300 Rorug07G0343400.1 Rorug07G0343500.1
rosa_samantha	Rh7AG498100 Rh7BG468700 Rh7CG514800 Rh7DG482200
rosa_wichuraiana	Rw7G042010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2511
Acc65I	GGTACC	1 cut(s)	1449
AccB1I	GGYRCC	1 cut(s)	1449
AccB7I	CCANNNNNTGG	2 cut(s)	446, 1229
AccI	GTMKAC	1 cut(s)	2052
AciI	CCGC	9 cut(s)	88, 496, 568, 1309, 1520, 2106, 2687, 2766, 2840
AclWI	GGATC	8 cut(s)	604, 617, 658, 731, 1125, 1138, 1189, 2371
AcoI	YGGCCR	3 cut(s)	1735, 2427, 2802
AcsI	RAATTY	6 cut(s)	765, 886, 1280, 1486, 2084, 2771
AcuI	CTGAAG	3 cut(s)	1155, 1697, 2895
AcyI	GRCGYC	1 cut(s)	352
AdeI	CACNNNGTG	2 cut(s)	446, 2492
AfaI	GTAC	4 cut(s)	966, 1236, 1313, 1451
AfiI	CCNNNNNNNGG	6 cut(s)	446, 1229, 1717, 1930, 2436, 2754
AflIII	ACRYGT	2 cut(s)	1104, 2586
AhlI	ACTAGT	1 cut(s)	1813
AjnI	CCWGG	4 cut(s)	229, 1563, 1973, 2271
AjuI	GAANNNNNNNTTGG	4 cut(s)	1716, 1748, 1917, 1949
Alw21I	GWGCWC	4 cut(s)	600, 1476, 1519, 1842
Alw26I	GTCTC	7 cut(s)	106, 1022, 1429, 1891, 2215, 2536, 2849
AlwI	GGATC	8 cut(s)	604, 617, 658, 731, 1125, 1138, 1189, 2371
AoxI	GGCC	5 cut(s)	1735, 1960, 2102, 2427, 2802
ApoI	RAATTY	6 cut(s)	765, 886, 1280, 1486, 2084, 2771
ArsI	GACNNNNNNTTYG	2 cut(s)	1961, 1993
AseI	ATTAAT	1 cut(s)	396
Asp700I	GAANNNNTTC	1 cut(s)	164
Asp718I	GGTACC	1 cut(s)	1449
AspLEI	GCGC	2 cut(s)	2497, 2796
AspS9I	GGNCC	4 cut(s)	1921, 2103, 2712, 2757
AsuC2I	CCSGG	1 cut(s)	1179
AsuHPI	GGTGA	7 cut(s)	17, 497, 590, 740, 1373, 2087, 2300
AvaII	GGWCC	3 cut(s)	1921, 2712, 2757
BaeGI	GKGCMC	2 cut(s)	1058, 1499
BalI	TGGCCA	2 cut(s)	1737, 2804
BamHI	GGATCC	2 cut(s)	609, 1130
BanI	GGYRCC	1 cut(s)	1449
BanII	GRGCYC	2 cut(s)	1476, 1842
BauI	CACGAG	2 cut(s)	2277, 2894
BbsI	GAAGAC	4 cut(s)	854, 1301, 2484, 2946
Bbv12I	GWGCWC	4 cut(s)	600, 1476, 1519, 1842
BceAI	ACGGC	1 cut(s)	2081
BciT130I	CCWGG	4 cut(s)	231, 1565, 1975, 2273
BciVI	GTATCC	1 cut(s)	2723
BclI	TGATCA	2 cut(s)	3, 2419
BcnI	CCSGG	1 cut(s)	1179
BcoDI	GTCTC	7 cut(s)	106, 1022, 1429, 1891, 2215, 2536, 2849
BcuI	ACTAGT	1 cut(s)	1813
BfaI	CTAG	1 cut(s)	1814
BfmI	CTRYAG	4 cut(s)	852, 1152, 2053, 2634
BfuI	GTATCC	1 cut(s)	2723
BglII	AGATCT	1 cut(s)	2777
BlpI	GCTNAGC	2 cut(s)	1841, 2919
Bme1390I	CCNGG	5 cut(s)	231, 1179, 1565, 1975, 2273
Bme18I	GGWCC	3 cut(s)	1921, 2712, 2757
BmgT120I	GGNCC	4 cut(s)	1921, 2103, 2712, 2757
BmiI	GGNNCC	6 cut(s)	330, 611, 1132, 1451, 1922, 2179
BmrFI	CCNGG	5 cut(s)	231, 1179, 1565, 1975, 2273
BmrI	ACTGGG	2 cut(s)	107, 995
BmuI	ACTGGG	2 cut(s)	107, 995
BpiI	GAAGAC	4 cut(s)	854, 1301, 2484, 2946
BpmI	CTGGAG	2 cut(s)	2640, 2712
Bpu10I	CCTNAGC	1 cut(s)	375
Bpu1102I	GCTNAGC	2 cut(s)	1841, 2919
BpuEI	CTTGAG	5 cut(s)	173, 1394, 1445, 1679, 1856
BpuMI	CCSGG	1 cut(s)	1179
BsaAI	YACGTR	2 cut(s)	1045, 2202
BsaHI	GRCGYC	1 cut(s)	352
BsaI	GGTCTC	1 cut(s)	2215
BsaJI	CCNNGG	7 cut(s)	229, 230, 605, 704, 1563, 1973, 2271
BsaWI	WCCGGW	1 cut(s)	2453
BsaXI	ACNNNNNCTCC	4 cut(s)	115, 145, 2846, 2876
Bsc4I	CCNNNNNNNGG	6 cut(s)	446, 1229, 1717, 1930, 2436, 2754
Bse1I	ACTGG	7 cut(s)	113, 665, 1001, 1418, 1523, 2077, 2430
Bse3DI	GCAATG	3 cut(s)	1200, 2127, 2252
BseBI	CCWGG	4 cut(s)	231, 1565, 1975, 2273
BseDI	CCNNGG	7 cut(s)	229, 230, 605, 704, 1563, 1973, 2271
BseLI	CCNNNNNNNGG	6 cut(s)	446, 1229, 1717, 1930, 2436, 2754
BseMI	GCAATG	3 cut(s)	1200, 2127, 2252
BseMII	CTCAG	2 cut(s)	1855, 2933
BseNI	ACTGG	7 cut(s)	113, 665, 1001, 1418, 1523, 2077, 2430
BseSI	GKGCMC	2 cut(s)	1058, 1499
BseYI	CCCAGC	1 cut(s)	1608
BshFI	GGCC	5 cut(s)	1737, 1962, 2104, 2429, 2804
BshNI	GGYRCC	1 cut(s)	1449
BsiHKAI	GWGCWC	4 cut(s)	600, 1476, 1519, 1842
BsiSI	CCGG	4 cut(s)	1179, 2430, 2454, 2936
BslFI	GGGAC	5 cut(s)	422, 671, 913, 1277, 1868
BslI	CCNNNNNNNGG	6 cut(s)	446, 1229, 1717, 1930, 2436, 2754
BsmAI	GTCTC	7 cut(s)	106, 1022, 1429, 1891, 2215, 2536, 2849
BsmBI	CGTCTC	1 cut(s)	2849
BsmFI	GGGAC	5 cut(s)	422, 671, 913, 1277, 1868
BsmI	GAATGC	1 cut(s)	199
BsnI	GGCC	5 cut(s)	1737, 1962, 2104, 2429, 2804
Bso31I	GGTCTC	1 cut(s)	2215
Bsp1286I	GDGCHC	6 cut(s)	600, 1058, 1476, 1499, 1519, 1842
Bsp1720I	GCTNAGC	2 cut(s)	1841, 2919
BspACI	CCGC	9 cut(s)	88, 496, 568, 1309, 1520, 2106, 2687, 2766, 2840
BspANI	GGCC	5 cut(s)	1737, 1962, 2104, 2429, 2804
BspCNI	CTCAG	2 cut(s)	1854, 2932
BspHI	TCATGA	2 cut(s)	1360, 1951
BspLI	GGNNCC	6 cut(s)	330, 611, 1132, 1451, 1922, 2179
BspMAI	CTGCAG	2 cut(s)	856, 1156
BspPI	GGATC	8 cut(s)	604, 617, 658, 731, 1125, 1138, 1189, 2371
BspQI	GCTCTTC	1 cut(s)	512
BspT107I	GGYRCC	1 cut(s)	1449
BspTNI	GGTCTC	1 cut(s)	2215
BsrDI	GCAATG	3 cut(s)	1200, 2127, 2252
BsrI	ACTGG	7 cut(s)	113, 665, 1001, 1418, 1523, 2077, 2430
BssECI	CCNNGG	7 cut(s)	229, 230, 605, 704, 1563, 1973, 2271
BssNI	GRCGYC	1 cut(s)	352
BssSI	CACGAG	2 cut(s)	2277, 2894
BssT1I	CCWWGG	2 cut(s)	605, 704
Bst2BI	CACGAG	2 cut(s)	2277, 2894
Bst2UI	CCWGG	4 cut(s)	231, 1565, 1975, 2273
Bst4CI	ACNGT	9 cut(s)	142, 595, 728, 1142, 1243, 1501, 1781, 2387, 2971
Bst6I	CTCTTC	3 cut(s)	512, 519, 1807
BstACI	GRCGYC	1 cut(s)	352
BstAPI	GCANNNNNTGC	3 cut(s)	1202, 1355, 2492
BstBAI	YACGTR	2 cut(s)	1045, 2202
BstC8I	GCNNGC	4 cut(s)	240, 956, 2106, 2259
BstDEI	CTNAG	7 cut(s)	375, 1098, 1317, 1841, 1890, 2034, 2919
BstENI	CCTNNNNNAGG	1 cut(s)	1715
BstHHI	GCGC	2 cut(s)	2497, 2796
BstMAI	GTCTC	7 cut(s)	106, 1022, 1429, 1891, 2215, 2536, 2849
BstNI	CCWGG	4 cut(s)	231, 1565, 1975, 2273
BstNSI	RCATGY	3 cut(s)	1108, 2590, 2794
BstSCI	CCNGG	5 cut(s)	229, 1177, 1563, 1973, 2271
BstSFI	CTRYAG	4 cut(s)	852, 1152, 2053, 2634
BstSLI	GKGCMC	2 cut(s)	1058, 1499
BstV2I	GAAGAC	4 cut(s)	854, 1301, 2484, 2946
BstX2I	RGATCY	5 cut(s)	609, 650, 1130, 2363, 2777
BstXI	CCANNNNNNTGG	2 cut(s)	120, 711
BstYI	RGATCY	5 cut(s)	609, 650, 1130, 2363, 2777
BsuI	GTATCC	1 cut(s)	2723
BsuRI	GGCC	5 cut(s)	1737, 1962, 2104, 2429, 2804
BtgZI	GCGATG	1 cut(s)	2169
BtsI	GCAGTG	3 cut(s)	866, 945, 2825
BtsIMutI	CAGTG	8 cut(s)	245, 866, 945, 1008, 1239, 2070, 2392, 2825
Cac8I	GCNNGC	4 cut(s)	240, 956, 2106, 2259
CciI	TCATGA	2 cut(s)	1360, 1951
CfoI	GCGC	2 cut(s)	2497, 2796
Cfr13I	GGNCC	4 cut(s)	1921, 2103, 2712, 2757
CseI	GACGC	4 cut(s)	341, 1197, 1582, 1610
Csp6I	GTAC	4 cut(s)	965, 1235, 1312, 1450
CviQI	GTAC	4 cut(s)	965, 1235, 1312, 1450
DdeI	CTNAG	7 cut(s)	375, 1098, 1317, 1841, 1890, 2034, 2919
DraI	TTTAAA	1 cut(s)	309
DraIII	CACNNNGTG	2 cut(s)	446, 2492
EaeI	YGGCCR	3 cut(s)	1735, 2427, 2802
Eam1104I	CTCTTC	3 cut(s)	512, 519, 1807
EarI	CTCTTC	3 cut(s)	512, 519, 1807
Ecl136II	GAGCTC	2 cut(s)	1474, 1840
Eco130I	CCWWGG	2 cut(s)	605, 704
Eco24I	GRGCYC	2 cut(s)	1476, 1842
Eco31I	GGTCTC	1 cut(s)	2215
Eco32I	GATATC	1 cut(s)	1957
Eco47I	GGWCC	3 cut(s)	1921, 2712, 2757
Eco53kI	GAGCTC	2 cut(s)	1474, 1840
Eco57I	CTGAAG	3 cut(s)	1155, 1697, 2895
EcoICRI	GAGCTC	2 cut(s)	1474, 1840
EcoNI	CCTNNNNNAGG	1 cut(s)	1715
EcoRI	GAATTC	2 cut(s)	1486, 2771
EcoRII	CCWGG	4 cut(s)	229, 1563, 1973, 2271
EcoRV	GATATC	1 cut(s)	1957
EcoT14I	CCWWGG	2 cut(s)	605, 704
EcoT38I	GRGCYC	2 cut(s)	1476, 1842
ErhI	CCWWGG	2 cut(s)	605, 704
Esp3I	CGTCTC	1 cut(s)	2849
FalI	AAGNNNNNCTT	2 cut(s)	2709, 2741
FaqI	GGGAC	5 cut(s)	422, 671, 913, 1277, 1868
FauI	CCCGC	3 cut(s)	81, 575, 2113
FauNDI	CATATG	1 cut(s)	1770
FbaI	TGATCA	2 cut(s)	3, 2419
FblI	GTMKAC	1 cut(s)	2052
FriOI	GRGCYC	2 cut(s)	1476, 1842
FspBI	CTAG	1 cut(s)	1814
GlaI	GCGC	2 cut(s)	2496, 2795
GsaI	CCCAGC	1 cut(s)	1612
GsuI	CTGGAG	2 cut(s)	2640, 2712
HaeIII	GGCC	5 cut(s)	1737, 1962, 2104, 2429, 2804
HapII	CCGG	4 cut(s)	1179, 2430, 2454, 2936
HgaI	GACGC	4 cut(s)	341, 1197, 1582, 1610
HhaI	GCGC	2 cut(s)	2497, 2796
Hin1I	GRCGYC	1 cut(s)	352
Hin6I	GCGC	2 cut(s)	2495, 2794
HinP1I	GCGC	2 cut(s)	2495, 2794
HincII	GTYRAC	2 cut(s)	226, 2584
HindII	GTYRAC	2 cut(s)	226, 2584
HindIII	AAGCTT	1 cut(s)	2751
HinfI	GANTC	9 cut(s)	206, 664, 1079, 1391, 2029, 2650, 2694, 2743, 2904
HpaII	CCGG	4 cut(s)	1179, 2430, 2454, 2936
HphI	GGTGA	7 cut(s)	17, 497, 590, 740, 1373, 2087, 2300
Hpy166II	GTNNAC	8 cut(s)	109, 226, 721, 731, 1042, 1669, 2053, 2584
Hpy8I	GTNNAC	8 cut(s)	109, 226, 721, 731, 1042, 1669, 2053, 2584
Hpy99I	CGWCG	1 cut(s)	728
HpyAV	CCTTC	8 cut(s)	375, 940, 1179, 1635, 1721, 1748, 2323, 2714
HpyCH4III	ACNGT	9 cut(s)	142, 595, 728, 1142, 1243, 1501, 1781, 2387, 2971
HpyCH4IV	ACGT	4 cut(s)	618, 1044, 1298, 2201
HpyF3I	CTNAG	7 cut(s)	375, 1098, 1317, 1841, 1890, 2034, 2919
HpySE526I	ACGT	4 cut(s)	618, 1044, 1298, 2201
Hsp92I	GRCGYC	1 cut(s)	352
HspAI	GCGC	2 cut(s)	2495, 2794
KpnI	GGTACC	1 cut(s)	1453
Ksp22I	TGATCA	2 cut(s)	3, 2419
LguI	GCTCTTC	1 cut(s)	512
LmnI	GCTCC	5 cut(s)	334, 1514, 1940, 2183, 2549
MaeI	CTAG	1 cut(s)	1814
MaeII	ACGT	4 cut(s)	618, 1044, 1298, 2201
MaeIII	GTNAC	7 cut(s)	862, 1003, 1243, 1456, 1781, 2288, 2623
MfeI	CAATTG	1 cut(s)	2466
MflI	RGATCY	5 cut(s)	609, 650, 1130, 2363, 2777
MhlI	GDGCHC	6 cut(s)	600, 1058, 1476, 1499, 1519, 1842
MlsI	TGGCCA	2 cut(s)	1737, 2804
MluNI	TGGCCA	2 cut(s)	1737, 2804
MlyI	GAGTC	3 cut(s)	1073, 2703, 2737
MmeI	TCCRAC	3 cut(s)	101, 874, 1873
Mox20I	TGGCCA	2 cut(s)	1737, 2804
MroXI	GAANNNNTTC	1 cut(s)	164
MscI	TGGCCA	2 cut(s)	1737, 2804
MslI	CAYNNNNRTG	2 cut(s)	250, 1258
Msp20I	TGGCCA	2 cut(s)	1737, 2804
MspA1I	CMGCKG	7 cut(s)	496, 857, 1346, 2108, 2766, 2842, 2873
MspI	CCGG	4 cut(s)	1179, 2430, 2454, 2936
MspR9I	CCNGG	5 cut(s)	231, 1179, 1565, 1975, 2273
MunI	CAATTG	1 cut(s)	2466
Mva1269I	GAATGC	1 cut(s)	199
MvaI	CCWGG	4 cut(s)	231, 1565, 1975, 2273
NciI	CCSGG	1 cut(s)	1179
NdeI	CATATG	1 cut(s)	1770
NlaIV	GGNNCC	6 cut(s)	330, 611, 1132, 1451, 1922, 2179
NmuCI	GTSAC	4 cut(s)	862, 1003, 1456, 2288
NspI	RCATGY	3 cut(s)	1108, 2590, 2794
PagI	TCATGA	2 cut(s)	1360, 1951
PasI	CCCWGGG	1 cut(s)	230
PciI	ACATGT	2 cut(s)	1104, 2586
PciSI	GCTCTTC	1 cut(s)	512
PcsI	WCGNNNNNNNCGW	2 cut(s)	201, 349
PctI	GAATGC	1 cut(s)	199
PdmI	GAANNNNTTC	1 cut(s)	164
PfeI	GAWTC	6 cut(s)	206, 664, 1391, 2029, 2650, 2904
PflMI	CCANNNNNTGG	2 cut(s)	446, 1229
PleI	GAGTC	3 cut(s)	1073, 2702, 2737
PpsI	GAGTC	3 cut(s)	1073, 2702, 2737
Ppu21I	YACGTR	2 cut(s)	1045, 2202
PscI	ACATGT	2 cut(s)	1104, 2586
PshBI	ATTAAT	1 cut(s)	396
PsiI	TTATAA	1 cut(s)	2511
Psp124BI	GAGCTC	2 cut(s)	1476, 1842
Psp6I	CCWGG	4 cut(s)	229, 1563, 1973, 2271
PspFI	CCCAGC	1 cut(s)	1608
PspGI	CCWGG	4 cut(s)	229, 1563, 1973, 2271
PspN4I	GGNNCC	6 cut(s)	330, 611, 1132, 1451, 1922, 2179
PspPI	GGNCC	4 cut(s)	1921, 2103, 2712, 2757
PsrI	GAACNNNNNNTAC	2 cut(s)	1304, 1336
PstI	CTGCAG	2 cut(s)	856, 1156
PsuI	RGATCY	5 cut(s)	609, 650, 1130, 2363, 2777
PvuII	CAGCTG	3 cut(s)	857, 1346, 2873
RsaI	GTAC	4 cut(s)	966, 1236, 1313, 1451
RsaNI	GTAC	4 cut(s)	965, 1235, 1312, 1450
RseI	CAYNNNNRTG	2 cut(s)	250, 1258
SacI	GAGCTC	2 cut(s)	1476, 1842
SapI	GCTCTTC	1 cut(s)	512
Sau96I	GGNCC	4 cut(s)	1921, 2103, 2712, 2757
SchI	GAGTC	3 cut(s)	1073, 2703, 2737
ScrFI	CCNGG	5 cut(s)	231, 1179, 1565, 1975, 2273
SduI	GDGCHC	6 cut(s)	600, 1058, 1476, 1499, 1519, 1842
SfcI	CTRYAG	4 cut(s)	852, 1152, 2053, 2634
SinI	GGWCC	3 cut(s)	1921, 2712, 2757
SmiMI	CAYNNNNRTG	2 cut(s)	250, 1258
SmlI	CTYRAG	5 cut(s)	152, 1373, 1424, 1658, 1835
SmoI	CTYRAG	5 cut(s)	152, 1373, 1424, 1658, 1835
SpeI	ACTAGT	1 cut(s)	1813
SsiI	CCGC	9 cut(s)	88, 496, 568, 1309, 1520, 2106, 2687, 2766, 2840
SspI	AATATT	1 cut(s)	1354
SspMI	CTAG	1 cut(s)	1814
SstI	GAGCTC	2 cut(s)	1476, 1842
StyD4I	CCNGG	5 cut(s)	229, 1177, 1563, 1973, 2271
StyI	CCWWGG	2 cut(s)	605, 704
TaaI	ACNGT	9 cut(s)	142, 595, 728, 1142, 1243, 1501, 1781, 2387, 2971
TaiI	ACGT	4 cut(s)	621, 1047, 1301, 2204
TaqI	TCGA	3 cut(s)	168, 2909, 2976
TaqII	GACCGA	1 cut(s)	2197
TauI	GCSGC	1 cut(s)	2690
TfiI	GAWTC	6 cut(s)	206, 664, 1391, 2029, 2650, 2904
TscAI	CASTG	8 cut(s)	252, 873, 945, 1008, 1246, 2077, 2392, 2825
TseFI	GTSAC	4 cut(s)	862, 1003, 1456, 2288
Tsp45I	GTSAC	4 cut(s)	862, 1003, 1456, 2288
TspDTI	ATGAA	9 cut(s)	311, 382, 959, 1242, 2394, 2490, 2669, 2715, 2937
TspGWI	ACGGA	3 cut(s)	2024, 2503, 2873
TspRI	CASTG	8 cut(s)	252, 873, 945, 1008, 1246, 2077, 2392, 2825
Van91I	CCANNNNNTGG	2 cut(s)	446, 1229
VpaK11BI	GGWCC	3 cut(s)	1921, 2712, 2757
VspI	ATTAAT	1 cut(s)	396
XagI	CCTNNNNNAGG	1 cut(s)	1715
XapI	RAATTY	6 cut(s)	765, 886, 1280, 1486, 2084, 2771
XceI	RCATGY	3 cut(s)	1108, 2590, 2794
XmiI	GTMKAC	1 cut(s)	2052
XmnI	GAANNNNTTC	1 cut(s)	164
XspI	CTAG	1 cut(s)	1814

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.