Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0005632

Physical Location & Seq

Genomic Coordinates Forward (+)

54027016 .. 54035374

Gene Structure 8359 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1440400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2865 bp

ATGCCTGAGAAGAAGATACTGGAGAATGGGTTTGCTGAAAGAGATCACGAGCTTTCGGATTCTTCACTTGAAAAGGTCGACCCTGGGAAGCCTGCATCACTGACATGGCAACGAAAATTAAACAGCAAGGGCAGTGCACCTTTGCTATTCACTCTAAGTTTAAAAGAGATAATTCATTTGGCTCCAATAGGTATTCGCCTATGGCGTCATTTACGAGAAGAAGCTGCTAACAAAAGGGAGGGCTTCATTGATCCCTTTGCCAAGCGCCATCTAACATCTAGTCATGGTGTCCCACTTGGTGGTATTGGGGCAGGAAGCATCGGAAGAAGTTACAGTGGGGATTTTCAGCGGTGGCAGCTGTTCCCTGGAAGATGTGAAGAGAAACCTGTTTTAGCAGATCAATTTTCTGTATTTGTTTCCCGCACCAATGGTGAAAAATATTCTACTGTGCTCTGTCCGAGGAGCCCAGATGATCTGAAAGAAAGTCAAGTTTCAGGGATTGGATCTTGGGACTGGAATCTGAACGGGGATAATTCTACATATTATGCCCTTTTTCCAAGGGCTTGGTCTGTTTATGACGGTGAACCTGACCCAGCTCTCAAAATAGTCTGTCGTCAAATTTCACCTTTTATCCCCCATAATTACAAGGAGAGCAGCCTACCTGTTTCAGTTTTTACTTATACACTATATAATTCTGGGAAGACTTCAGCTGATGTCACACTGCTTTTCACCTGGGCAAATTCTGTTGGAGGGCTTTCTGAATTTTCTGGTCATCACTCCAATTCAAGAGCATTGATGAAGGATGGTGTGCATGGCGTACTTCTACATCACAAATACAAGACTGCAAGTGGGCTGTCCCCAGTGACATTTGCAATAGCAGCACAGGAGACTGATGGTGTTCACGTATCAGAGTGTCCTTGCTTTGTAATATCTGGTGACTCAAAGGGTGTCACGGCTAAGGACATGTGGAGTGAGATAAAAGAGCATGGATCCTTTGATCGTCTTAATTCTGCAGAAATGCCATTACCTTCAGAACCCGGATCATCTATTGGGGCAGCCATTGCTGCGTCTGTGGCAGTTCCATCTGGTGAAGTACGTACTGTAACATTTTCATTGGCATGGGATTGCCCAGAAGCAAAATTTACGGGTGGGAAGACGTATCACAGGCGGTACACTAAATTTTATGGAACACATGGGGAAGCTGCTGCAAATATTGCTCATGATGCTATTCTTGAGCATCGCCATTGGGAATCCCAGATTGAAGCATGGCAAAGACCAGTTCTTGAGGACAAGAGACTTCCAGAATGGTACCCTGTCACTCTGTTCAATGAGCTATACTTTCTAAATTCTGGGGGCACAGTTTGGACAGATGGATCACCTGCAGTCCATAGCTTAAGAAGTATTATAGAAAGGAAGTTTTCCCTTGATAAGTCCTCATTAGGTTTGAAAAGCATCATTGATGCACCCGAACAAAATGATACTGCTATCGACATTCTTGGAAGGATGACATCAACACTTGAGCAAGTTCACATGCCCATTGCAGCAAACTCTGCATTTGGAACAAATCTCCTTCAGGAGGGAGAAGAAAACATTGGTCAATTCCTTTATCTTGAAGGAATTGAATATCAGATGTGGAATACTTACGATGTCCATTTCTACTCATCTTTTGCACTAGTCATGCTATTTCCAAAACTTCAACTCAGCATCCAACGAGACTTTGCAGCAGCAGTAATGATGCATGACCCCAGTAAAATGAGACTTCTCTGTGATGGAACATGGGTCCAAAGAAAGGTTCTTGGAGCTGTTCCTCATGATATCGGCCTCCATGACCCCTGGTTTGAAGTAAATGCTTATAACCTGTATAACACAGATAGGTGGAAAGACTTGAATCCTAAGTTTGTTCTCCAAGTTTACAGGGATGTGGTTGCCACGGGTGATAAGAAGTTTGCAGAAGCCGTTTGGCCCGCTGTTTATGTTGCAATGGCTTATATGGAACAATTTGATAAAGATGGAGATGGAATGATTGAGAATGATGGCTTCCCAGATCAGACTTATGATACGTGGTCAGTCTATGGTGTGAGTGCATATAGTGGTGGGCTGTGGGTAGCAGCATTGCAGGCTGCATCAGCCATGGCACGAGAAGTAGGTGACAAGGGTTCAGAAGTTTATTTTTGGCAAAAGTTTCAGAAGGCAAAAGCCGTGTATGCGAAATTATGGAATGGATCTTACTTTAATTATGACAACAGTGGTCAAGCTTCTAGTTCATCAATTCAAGCTGATCAACTGGCCGGACAATGGTATGCCAGAGCATGCGGTCTTTTGCCAATTGTTGACGAAGACAAGGCACGAACTGCACTGGAAAAGGTTTATAATTACAATGTCCTAAAGTTTAAGGATGGAAGACGAGGGGCTGTTAACGGGATGCTACCTAATGGAAAGGTTGACATGTCAACATTGCAGTCAAGAGAAATATGGTCCGGAGTTACATACGCTGTAGCTGCAACAATGATTCAGGAAGATGCAATTGATATGGGATTTCATACTGCGGAGGGAATTTATGAAGCTGGATGGTCCAAAGAAGGACTTGGATACTCTTTTCAGACTCCTGAAGCTTGGACCACCAGCGGCGAATACAGATCTTTAGCTTACATGCGCCCTCTGGCGATTTGGTCAATGCAGTGGGCTTTAACAAAACCACCGCTGTTCAAGCAGGAGACGGAATTAGAAGTGGATGAAGTTATTTTGCTTAGGCACAAGGCTGGATTCTCGAAAGTGGCTCAGCTTCTAAAGTTGCCACCAGACGAATCGTCAAGAAGTATTTTGCAGACTGTTTTCGATTATACCTGCAAGAGGATGTGGCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004348 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005790 GO:0005886 GO:0006082 GO:0006629 GO:0006643 GO:0006664 GO:0006665 GO:0006687 GO:0006807 GO:0007275 GO:0007399 GO:0007417 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008422 GO:0009056 GO:0009987 GO:0012505 GO:0015926 GO:0016020 GO:0016021 GO:0016137 GO:0016139 GO:0016787 GO:0016798 GO:0019752 GO:0021953 GO:0021954 GO:0022008 GO:0030154 GO:0030182 GO:0031224 GO:0032501 GO:0032502 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0048468 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0071704 GO:0071944 GO:1901135 GO:1901136 GO:1901360 GO:1901564 GO:1901575 GO:1901615 GO:1901657 GO:1901658 GO:1903509

KEGG Pathways

Metabolic & Signaling

ko00511 ko00600 ko01100 map00511 map00600 map01100

Pfam Domains

Protein Families

DUF608 Glyco_hydr_116N

Protein Analysis

955

Amino Acids

106.69

Weight (kDa)

5.8

Isoelectric Point (pI)

41.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydr_116N	PF12215	96 - 416	4.3e-112	beta-glucosidase 2, glycosyl-hydrolase family 116 N-term
DUF608	PF04685	532 - 892	1.4e-166	Glycosyl-hydrolase family 116, catalytic region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005632)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49900 AT5G49900 AT5G49900
fragaria_vesca	FvH4_5g39140 FvH4_5g39140 FvH4_5g39140 FvH4_5g39140 FvH4_5g39140
malus_domestica	MD08G1245400.v1.1 MD15G1440400.v1.1
prunus_persica	Prupe.1G582500_v2.0.a1 Prupe.1G582500_v2.0.a1
pyrus_communis	pycom08g21280 pycom15g38820
rosa_chinensis	RchiOBHm_Chr7g0241141
rosa_laevigata	RLG00000000592
rosa_multiflora	Rmu_sc0006665.1_g000004
rosa_roxburghii	Rroxscaffold_3G00219230
rosa_rugosa	Rorug07G0343100 Rorug07G0343200 Rorug07G0343300 Rorug07G0343400.1 Rorug07G0343500.1
rosa_samantha	Rh7AG498100 Rh7BG468700 Rh7CG514800 Rh7DG482200
rosa_wichuraiana	Rw7G042010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1854, 2370
AarI	CACCTGC	1 cut(s)	1387
Acc36I	ACCTGC	2 cut(s)	1387, 2852
Acc65I	GGTACC	1 cut(s)	1308
AccB1I	GGYRCC	1 cut(s)	1308
AccB7I	CCANNNNNTGG	1 cut(s)	299
AccI	GTMKAC	1 cut(s)	78
AccIII	TCCGGA	1 cut(s)	2477
AciI	CCGC	8 cut(s)	349, 421, 1168, 1965, 2313, 2546, 2625, 2699
AclWI	GGATC	7 cut(s)	245, 511, 984, 997, 1048, 1381, 2230
AcoI	YGGCCR	1 cut(s)	2286
AcsI	RAATTY	7 cut(s)	618, 739, 761, 1139, 1178, 1345, 2553
AcuI	CTGAAG	4 cut(s)	690, 1014, 1556, 2628
AcyI	GRCGYC	1 cut(s)	205
AdeI	CACNNNGTG	1 cut(s)	299
AfaI	GTAC	5 cut(s)	819, 1095, 1099, 1172, 1310
AfiI	CCNNNNNNNGG	5 cut(s)	299, 1576, 1789, 2295, 2850
AflII	CTTAAG	1 cut(s)	1393
AflIII	ACRYGT	2 cut(s)	963, 2445
AhdI	GACNNNNNGTC	1 cut(s)	2806
AhlI	ACTAGT	1 cut(s)	1672
AjnI	CCWGG	4 cut(s)	82, 364, 731, 1832
AjuI	GAANNNNNNNTTGG	4 cut(s)	1575, 1607, 1776, 1808
Alw21I	GWGCWC	2 cut(s)	139, 453
Alw26I	GTCTC	5 cut(s)	881, 1288, 1707, 1750, 2708
Alw44I	GTGCAC	1 cut(s)	135
AlwI	GGATC	7 cut(s)	245, 511, 984, 997, 1048, 1381, 2230
Aor13HI	TCCGGA	1 cut(s)	2477
AoxI	GGCC	3 cut(s)	1819, 1961, 2286
ApaLI	GTGCAC	1 cut(s)	135
ApoI	RAATTY	7 cut(s)	618, 739, 761, 1139, 1178, 1345, 2553
ArsI	GACNNNNNNTTYG	2 cut(s)	1820, 1852
Asp718I	GGTACC	1 cut(s)	1308
AspLEI	GCGC	2 cut(s)	267, 2655
AspS9I	GGNCC	5 cut(s)	1780, 1962, 2475, 2571, 2616
AsuC2I	CCSGG	1 cut(s)	1038
AsuHPI	GGTGA	9 cut(s)	443, 593, 615, 721, 947, 1100, 1368, 1946, 2159
AvaII	GGWCC	4 cut(s)	1780, 2475, 2571, 2616
BaeGI	GKGCMC	2 cut(s)	139, 1358
BamHI	GGATCC	1 cut(s)	989
BanI	GGYRCC	1 cut(s)	1308
BanII	GRGCYC	1 cut(s)	467
BauI	CACGAG	2 cut(s)	47, 2136
BbsI	GAAGAC	4 cut(s)	707, 1160, 2343, 2407
Bbv12I	GWGCWC	2 cut(s)	139, 453
BceAI	ACGGC	3 cut(s)	969, 1940, 2183
BciT130I	CCWGG	4 cut(s)	84, 366, 733, 1834
BciVI	GTATCC	1 cut(s)	2582
BclI	TGATCA	1 cut(s)	2278
BcnI	CCSGG	1 cut(s)	1038
BcoDI	GTCTC	5 cut(s)	881, 1288, 1707, 1750, 2708
BcuI	ACTAGT	1 cut(s)	1672
BfaI	CTAG	3 cut(s)	279, 1673, 2259
BfmI	CTRYAG	3 cut(s)	1011, 1380, 2493
BfoI	RGCGCY	1 cut(s)	268
BfrI	CTTAAG	1 cut(s)	1393
BfuAI	ACCTGC	2 cut(s)	1387, 2852
BfuI	GTATCC	1 cut(s)	2582
BglII	AGATCT	1 cut(s)	2636
BlpI	GCTNAGC	1 cut(s)	2778
Bme1390I	CCNGG	5 cut(s)	84, 366, 733, 1038, 1834
Bme18I	GGWCC	4 cut(s)	1780, 2475, 2571, 2616
BmeRI	GACNNNNNGTC	1 cut(s)	2806
BmgT120I	GGNCC	5 cut(s)	1780, 1962, 2475, 2571, 2616
BmiI	GGNNCC	5 cut(s)	183, 464, 991, 1310, 1781
BmrFI	CCNGG	5 cut(s)	84, 366, 733, 1038, 1834
BmrI	ACTGGG	2 cut(s)	854, 1740
BmuI	ACTGGG	2 cut(s)	854, 1740
BpiI	GAAGAC	4 cut(s)	707, 1160, 2343, 2407
BpmI	CTGGAG	1 cut(s)	41
Bpu10I	CCTNAGC	2 cut(s)	957, 2747
Bpu1102I	GCTNAGC	1 cut(s)	2778
BpuEI	CTTGAG	3 cut(s)	1253, 1304, 1538
BpuMI	CCSGG	1 cut(s)	1038
BsaAI	YACGTR	3 cut(s)	904, 1097, 2061
BsaHI	GRCGYC	1 cut(s)	205
BsaJI	CCNNGG	9 cut(s)	82, 83, 364, 458, 557, 732, 1832, 1929, 2130
BsaWI	WCCGGW	1 cut(s)	2477
BsaXI	ACNNNNNCTCC	2 cut(s)	14, 44
Bsc4I	CCNNNNNNNGG	5 cut(s)	299, 1576, 1789, 2295, 2850
Bse1I	ACTGG	7 cut(s)	24, 518, 860, 1277, 1746, 2289, 2361
Bse3DI	GCAATG	5 cut(s)	1059, 1536, 1986, 2111, 2453
BseAI	TCCGGA	1 cut(s)	2477
BseBI	CCWGG	4 cut(s)	84, 366, 733, 1834
BseDI	CCNNGG	9 cut(s)	82, 83, 364, 458, 557, 732, 1832, 1929, 2130
BseGI	GGATG	9 cut(s)	808, 1509, 1704, 1924, 2401, 2427, 2573, 2737, 2859
BseLI	CCNNNNNNNGG	5 cut(s)	299, 1576, 1789, 2295, 2850
BseMI	GCAATG	5 cut(s)	1059, 1536, 1986, 2111, 2453
BseMII	CTCAG	2 cut(s)	1714, 2792
BseNI	ACTGG	7 cut(s)	24, 518, 860, 1277, 1746, 2289, 2361
BseRI	GAGGAG	1 cut(s)	475
BseSI	GKGCMC	2 cut(s)	139, 1358
BseYI	CCCAGC	1 cut(s)	592
BsgI	GTGCAG	1 cut(s)	2337
BshFI	GGCC	3 cut(s)	1821, 1963, 2288
BshNI	GGYRCC	1 cut(s)	1308
BsiHKAI	GWGCWC	2 cut(s)	139, 453
BsiSI	CCGG	3 cut(s)	1038, 2289, 2478
BslFI	GGGAC	3 cut(s)	275, 524, 841
BslI	CCNNNNNNNGG	5 cut(s)	299, 1576, 1789, 2295, 2850
BsmAI	GTCTC	5 cut(s)	881, 1288, 1707, 1750, 2708
BsmBI	CGTCTC	1 cut(s)	2708
BsmFI	GGGAC	3 cut(s)	275, 524, 841
BsnI	GGCC	3 cut(s)	1821, 1963, 2288
Bsp1286I	GDGCHC	4 cut(s)	139, 453, 467, 1358
Bsp13I	TCCGGA	1 cut(s)	2477
Bsp1720I	GCTNAGC	1 cut(s)	2778
Bsp19I	CCATGG	1 cut(s)	2130
BspACI	CCGC	8 cut(s)	349, 421, 1168, 1965, 2313, 2546, 2625, 2699
BspANI	GGCC	3 cut(s)	1821, 1963, 2288
BspCNI	CTCAG	2 cut(s)	1713, 2791
BspEI	TCCGGA	1 cut(s)	2477
BspHI	TCATGA	2 cut(s)	1219, 1810
BspLI	GGNNCC	5 cut(s)	183, 464, 991, 1310, 1781
BspMAI	CTGCAG	2 cut(s)	1015, 1384
BspMI	ACCTGC	2 cut(s)	1387, 2852
BspPI	GGATC	7 cut(s)	245, 511, 984, 997, 1048, 1381, 2230
BspT107I	GGYRCC	1 cut(s)	1308
BspTI	CTTAAG	1 cut(s)	1393
BsrDI	GCAATG	5 cut(s)	1059, 1536, 1986, 2111, 2453
BsrI	ACTGG	7 cut(s)	24, 518, 860, 1277, 1746, 2289, 2361
BssECI	CCNNGG	9 cut(s)	82, 83, 364, 458, 557, 732, 1832, 1929, 2130
BssNI	GRCGYC	1 cut(s)	205
BssSI	CACGAG	2 cut(s)	47, 2136
BssT1I	CCWWGG	2 cut(s)	557, 2130
Bst2BI	CACGAG	2 cut(s)	47, 2136
Bst2UI	CCWGG	4 cut(s)	84, 366, 733, 1834
Bst4CI	ACNGT	7 cut(s)	335, 448, 581, 1102, 1360, 2246, 2830
Bst6I	CTCTTC	1 cut(s)	372
BstACI	GRCGYC	1 cut(s)	205
BstAFI	CTTAAG	1 cut(s)	1393
BstAPI	GCANNNNNTGC	4 cut(s)	1061, 1214, 1550, 2351
BstBAI	YACGTR	3 cut(s)	904, 1097, 2061
BstC8I	GCNNGC	4 cut(s)	93, 1965, 2118, 2311
BstDEI	CTNAG	7 cut(s)	6, 155, 957, 1700, 1893, 2747, 2778
BstDSI	CCRYGG	2 cut(s)	1929, 2130
BstENI	CCTNNNNNAGG	2 cut(s)	1574, 2848
BstF5I	GGATG	9 cut(s)	808, 1509, 1704, 1924, 2401, 2427, 2573, 2737, 2859
BstH2I	RGCGCY	1 cut(s)	268
BstHHI	GCGC	2 cut(s)	267, 2655
BstMAI	GTCTC	5 cut(s)	881, 1288, 1707, 1750, 2708
BstNI	CCWGG	4 cut(s)	84, 366, 733, 1834
BstNSI	RCATGY	5 cut(s)	967, 1534, 2313, 2449, 2653
BstSCI	CCNGG	5 cut(s)	82, 364, 731, 1036, 1832
BstSFI	CTRYAG	3 cut(s)	1011, 1380, 2493
BstSLI	GKGCMC	2 cut(s)	139, 1358
BstSNI	TACGTA	1 cut(s)	1097
BstV2I	GAAGAC	4 cut(s)	707, 1160, 2343, 2407
BstX2I	RGATCY	4 cut(s)	503, 989, 2222, 2636
BstXI	CCANNNNNNTGG	1 cut(s)	564
BstYI	RGATCY	4 cut(s)	503, 989, 2222, 2636
BsuI	GTATCC	1 cut(s)	2582
BsuRI	GGCC	3 cut(s)	1821, 1963, 2288
BtgI	CCRYGG	2 cut(s)	1929, 2130
BtgZI	GCGATG	1 cut(s)	1223
BtsCI	GGATG	9 cut(s)	808, 1509, 1704, 1924, 2401, 2427, 2573, 2737, 2859
BtsI	GCAGTG	3 cut(s)	139, 719, 2684
BtsIMutI	CAGTG	8 cut(s)	98, 139, 340, 719, 867, 2251, 2354, 2684
BveI	ACCTGC	2 cut(s)	1387, 2852
Cac8I	GCNNGC	4 cut(s)	93, 1965, 2118, 2311
CciI	TCATGA	2 cut(s)	1219, 1810
CfoI	GCGC	2 cut(s)	267, 2655
Cfr13I	GGNCC	5 cut(s)	1780, 1962, 2475, 2571, 2616
CseI	GACGC	2 cut(s)	194, 1056
Csp6I	GTAC	5 cut(s)	818, 1094, 1098, 1171, 1309
CviQI	GTAC	5 cut(s)	818, 1094, 1098, 1171, 1309
DdeI	CTNAG	7 cut(s)	6, 155, 957, 1700, 1893, 2747, 2778
DraI	TTTAAA	1 cut(s)	162
DraIII	CACNNNGTG	1 cut(s)	299
DriI	GACNNNNNGTC	1 cut(s)	2806
EaeI	YGGCCR	1 cut(s)	2286
Eam1104I	CTCTTC	1 cut(s)	372
Eam1105I	GACNNNNNGTC	1 cut(s)	2806
EarI	CTCTTC	1 cut(s)	372
Eco105I	TACGTA	1 cut(s)	1097
Eco130I	CCWWGG	2 cut(s)	557, 2130
Eco24I	GRGCYC	1 cut(s)	467
Eco32I	GATATC	1 cut(s)	1816
Eco47I	GGWCC	4 cut(s)	1780, 2475, 2571, 2616
Eco57I	CTGAAG	4 cut(s)	690, 1014, 1556, 2628
EcoNI	CCTNNNNNAGG	2 cut(s)	1574, 2848
EcoRII	CCWGG	4 cut(s)	82, 364, 731, 1832
EcoRV	GATATC	1 cut(s)	1816
EcoT14I	CCWWGG	2 cut(s)	557, 2130
EcoT22I	ATGCAT	1 cut(s)	1740
EcoT38I	GRGCYC	1 cut(s)	467
ErhI	CCWWGG	2 cut(s)	557, 2130
Esp3I	CGTCTC	1 cut(s)	2708
FalI	AAGNNNNNCTT	2 cut(s)	2568, 2600
FaqI	GGGAC	3 cut(s)	275, 524, 841
FauI	CCCGC	2 cut(s)	428, 1972
FbaI	TGATCA	1 cut(s)	2278
FblI	GTMKAC	1 cut(s)	78
FokI	GGATG	8 cut(s)	815, 1516, 1691, 1931, 2408, 2434, 2580, 2744
FriOI	GRGCYC	1 cut(s)	467
FspBI	CTAG	3 cut(s)	279, 1673, 2259
GlaI	GCGC	2 cut(s)	266, 2654
GsaI	CCCAGC	1 cut(s)	596
GsuI	CTGGAG	1 cut(s)	41
HaeII	RGCGCY	1 cut(s)	268
HaeIII	GGCC	3 cut(s)	1821, 1963, 2288
HapII	CCGG	3 cut(s)	1038, 2289, 2478
HgaI	GACGC	2 cut(s)	194, 1056
HhaI	GCGC	2 cut(s)	267, 2655
Hin1I	GRCGYC	1 cut(s)	205
Hin6I	GCGC	2 cut(s)	265, 2653
HinP1I	GCGC	2 cut(s)	265, 2653
HincII	GTYRAC	5 cut(s)	79, 2332, 2416, 2443, 2451
HindII	GTYRAC	5 cut(s)	79, 2332, 2416, 2443, 2451
HindIII	AAGCTT	2 cut(s)	2253, 2610
HinfI	GANTC	9 cut(s)	59, 517, 938, 1250, 1888, 2509, 2602, 2763, 2804
HpaI	GTTAAC	1 cut(s)	2416
HpaII	CCGG	3 cut(s)	1038, 2289, 2478
HphI	GGTGA	9 cut(s)	443, 593, 615, 721, 947, 1100, 1368, 1946, 2159
HpyAV	CCTTC	7 cut(s)	793, 1038, 1494, 1580, 1607, 2182, 2573
HpyCH4III	ACNGT	7 cut(s)	335, 448, 581, 1102, 1360, 2246, 2830
HpyCH4IV	ACGT	4 cut(s)	903, 1096, 1157, 2060
HpyF3I	CTNAG	7 cut(s)	6, 155, 957, 1700, 1893, 2747, 2778
HpySE526I	ACGT	4 cut(s)	903, 1096, 1157, 2060
Hsp92I	GRCGYC	1 cut(s)	205
HspAI	GCGC	2 cut(s)	265, 2653
Kpn2I	TCCGGA	1 cut(s)	2477
KpnI	GGTACC	1 cut(s)	1312
Ksp22I	TGATCA	1 cut(s)	2278
KspAI	GTTAAC	1 cut(s)	2416
LmnI	GCTCC	3 cut(s)	187, 462, 1799
MaeI	CTAG	3 cut(s)	279, 1673, 2259
MaeII	ACGT	4 cut(s)	903, 1096, 1157, 2060
MaeIII	GTNAC	9 cut(s)	329, 715, 862, 935, 949, 1102, 1315, 2147, 2482
MfeI	CAATTG	2 cut(s)	2325, 2523
MflI	RGATCY	4 cut(s)	503, 989, 2222, 2636
MhlI	GDGCHC	4 cut(s)	139, 453, 467, 1358
MlyI	GAGTC	2 cut(s)	932, 2596
MmeI	TCCRAC	2 cut(s)	727, 1732
Mph1103I	ATGCAT	1 cut(s)	1740
MroI	TCCGGA	1 cut(s)	2477
MseI	TTAA	8 cut(s)	119, 161, 1005, 1394, 2232, 2391, 2415, 2687
MslI	CAYNNNNRTG	2 cut(s)	103, 1117
MspA1I	CMGCKG	6 cut(s)	349, 358, 710, 1967, 2625, 2701
MspCI	CTTAAG	1 cut(s)	1393
MspI	CCGG	3 cut(s)	1038, 2289, 2478
MspR9I	CCNGG	5 cut(s)	84, 366, 733, 1038, 1834
MunI	CAATTG	2 cut(s)	2325, 2523
MvaI	CCWGG	4 cut(s)	84, 366, 733, 1834
NciI	CCSGG	1 cut(s)	1038
NcoI	CCATGG	1 cut(s)	2130
NlaIV	GGNNCC	5 cut(s)	183, 464, 991, 1310, 1781
NmuCI	GTSAC	6 cut(s)	715, 862, 935, 949, 1315, 2147
NsiI	ATGCAT	1 cut(s)	1740
NspI	RCATGY	5 cut(s)	967, 1534, 2313, 2449, 2653
PaeI	GCATGC	1 cut(s)	2313
PagI	TCATGA	2 cut(s)	1219, 1810
PaqCI	CACCTGC	1 cut(s)	1387
PasI	CCCWGGG	1 cut(s)	83
PciI	ACATGT	2 cut(s)	963, 2445
PcsI	WCGNNNNNNNCGW	1 cut(s)	202
PfeI	GAWTC	7 cut(s)	59, 517, 1250, 1888, 2509, 2763, 2804
PflMI	CCANNNNNTGG	1 cut(s)	299
PleI	GAGTC	2 cut(s)	932, 2596
PpsI	GAGTC	2 cut(s)	932, 2596
Ppu21I	YACGTR	3 cut(s)	904, 1097, 2061
PscI	ACATGT	2 cut(s)	963, 2445
PsiI	TTATAA	2 cut(s)	1854, 2370
Psp6I	CCWGG	4 cut(s)	82, 364, 731, 1832
PspFI	CCCAGC	1 cut(s)	592
PspGI	CCWGG	4 cut(s)	82, 364, 731, 1832
PspN4I	GGNNCC	5 cut(s)	183, 464, 991, 1310, 1781
PspPI	GGNCC	5 cut(s)	1780, 1962, 2475, 2571, 2616
PstI	CTGCAG	2 cut(s)	1015, 1384
PsuI	RGATCY	4 cut(s)	503, 989, 2222, 2636
PvuII	CAGCTG	2 cut(s)	358, 710
RsaI	GTAC	5 cut(s)	819, 1095, 1099, 1172, 1310
RsaNI	GTAC	5 cut(s)	818, 1094, 1098, 1171, 1309
RseI	CAYNNNNRTG	2 cut(s)	103, 1117
SalI	GTCGAC	1 cut(s)	77
SaqAI	TTAA	8 cut(s)	119, 161, 1005, 1394, 2232, 2391, 2415, 2687
Sau96I	GGNCC	5 cut(s)	1780, 1962, 2475, 2571, 2616
SchI	GAGTC	2 cut(s)	932, 2596
ScrFI	CCNGG	5 cut(s)	84, 366, 733, 1038, 1834
SduI	GDGCHC	4 cut(s)	139, 453, 467, 1358
SfcI	CTRYAG	3 cut(s)	1011, 1380, 2493
SinI	GGWCC	4 cut(s)	1780, 2475, 2571, 2616
SmiMI	CAYNNNNRTG	2 cut(s)	103, 1117
SmlI	CTYRAG	4 cut(s)	1232, 1283, 1393, 1517
SmoI	CTYRAG	4 cut(s)	1232, 1283, 1393, 1517
SnaBI	TACGTA	1 cut(s)	1097
SpeI	ACTAGT	1 cut(s)	1672
SphI	GCATGC	1 cut(s)	2313
SsiI	CCGC	8 cut(s)	349, 421, 1168, 1965, 2313, 2546, 2625, 2699
SspI	AATATT	2 cut(s)	440, 1213
SspMI	CTAG	3 cut(s)	279, 1673, 2259
StyD4I	CCNGG	5 cut(s)	82, 364, 731, 1036, 1832
StyI	CCWWGG	2 cut(s)	557, 2130
TaaI	ACNGT	7 cut(s)	335, 448, 581, 1102, 1360, 2246, 2830
TaiI	ACGT	4 cut(s)	906, 1099, 1160, 2063
TaqI	TCGA	4 cut(s)	78, 1488, 2768, 2835
TauI	GCSGC	1 cut(s)	2628
TfiI	GAWTC	7 cut(s)	59, 517, 1250, 1888, 2509, 2763, 2804
Tru1I	TTAA	8 cut(s)	119, 161, 1005, 1394, 2232, 2391, 2415, 2687
Tru9I	TTAA	8 cut(s)	119, 161, 1005, 1394, 2232, 2391, 2415, 2687
TscAI	CASTG	8 cut(s)	105, 139, 340, 726, 867, 2251, 2361, 2684
TseFI	GTSAC	6 cut(s)	715, 862, 935, 949, 1315, 2147
Tsp45I	GTSAC	6 cut(s)	715, 862, 935, 949, 1315, 2147
TspDTI	ATGAA	8 cut(s)	164, 235, 812, 1101, 2253, 2528, 2574, 2748
TspGWI	ACGGA	1 cut(s)	2732
TspRI	CASTG	8 cut(s)	105, 139, 340, 726, 867, 2251, 2361, 2684
Van91I	CCANNNNNTGG	1 cut(s)	299
Vha464I	CTTAAG	1 cut(s)	1393
VneI	GTGCAC	1 cut(s)	135
VpaK11BI	GGWCC	4 cut(s)	1780, 2475, 2571, 2616
XagI	CCTNNNNNAGG	2 cut(s)	1574, 2848
XapI	RAATTY	7 cut(s)	618, 739, 761, 1139, 1178, 1345, 2553
XceI	RCATGY	5 cut(s)	967, 1534, 2313, 2449, 2653
XmiI	GTMKAC	1 cut(s)	78
XspI	CTAG	3 cut(s)	279, 1673, 2259
Zsp2I	ATGCAT	1 cut(s)	1740

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.