Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0014986

Physical Location & Seq

Genomic Coordinates Forward (+)

31363858 .. 31368317

Gene Structure 4460 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1249400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 762 bp

ATGATTTTTTATTTTTTTTGTTTGATGATGCAGAAACCAGAGTCTAGACTAATAGTGAAAGAGAAGAGCGTGTCGGTGATTCTTTTGGCTGGAGGGCAGGGCAAAAGGATGGGGGCCAGCATGCCAAAGCAATATCTTCCCCTTTTAGGCCAGCCAATTGCATTATACAGCTTCTACACTTTCTCCCAAATGATTGAAGTAAAGGAGATAATTGTAGTTTGTGATCCCTCCTGTAAGGATGTTTTTGAAGACGCCAAACCTAAGATCCAGAAGGAACTTAAATTCACACTACCAGGGAAGGAAAGACAAGATTCCGTTTACAGTGGACTCCAGGCAATTGATTTGACATCTGAGCTTGTTTGTATTCATGATTCTGCTAGACCTCTGGTATCATCAGGAGATGTAGAGAAGGTTCTCAAAGATGGTTGCTTAAATGGTGCAGCTGTACTTGGTGTTCCAGTAAAAGCTACAATCAAAGAGGCAAACAGTGAATCTTATGTAGTCAAAACCCTTGACAGGAGAACACTTTGGGAAATGCAGACACCCCAGGTCATCAAAACTCAGTTGCTTAAAGAGGGTTTTGAGCTTGTCAATAGGGAAGGCCTTGAAGTGACGGACGACGTGTCTATTGTGGAGCACCTTCAGCATCCGGTGTACATCACCGAAGGATCTTATACCAACATTAAGGTGACAACGCCGGATGATTTATTACTCGCGGAAAGAATGTTAAGTATGAGCTCGGGAAAGTCTTCTTCTGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006081 GO:0006082 GO:0006090 GO:0006629 GO:0006644 GO:0006720 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0008654 GO:0009058 GO:0009240 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016740 GO:0016772 GO:0016779 GO:0019288 GO:0019637 GO:0019682 GO:0019752 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046490 GO:0050518 GO:0070567 GO:0071704 GO:0090407 GO:1901135 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00900 ko01100 ko01110 ko01130 map00900 map01100 map01110 map01130

Pfam Domains

Protein Families

IspD

Protein Analysis

254

Amino Acids

28.17

Weight (kDa)

5.58

Isoelectric Point (pI)

42.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IspD	PF01128	24 - 243	1.4e-56	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
NTP_transf_3	PF12804	26 - 140	7e-09	MobA-like NTP transferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014986)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G02500 AT2G02500
fragaria_vesca	FvH4_5g27480
malus_domestica	MD08G1249400.v1.1 MD15G1444200.v1.1
prunus_persica	Prupe.1G586600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0218881
rosa_laevigata	RLG00000002347
rosa_multiflora	Rmu_sc0003823.1_g000002
rosa_roxburghii	Rroxscaffold_3G00240430
rosa_rugosa	Rorug07G0179400
rosa_samantha	Rh7AG322700 Rh7BG313800 Rh7CG339500 Rh7DG319900
rosa_wichuraiana	Rw7G027320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	716
AciI	CCGC	1 cut(s)	716
AclWI	GGATC	3 cut(s)	218, 259, 676
AcsI	RAATTY	1 cut(s)	281
AcuI	CTGAAG	1 cut(s)	626
AcyI	GRCGYC	1 cut(s)	252
AfaI	GTAC	2 cut(s)	447, 656
AfiI	CCNNNNNNNGG	2 cut(s)	146, 516
AflIII	ACRYGT	1 cut(s)	621
AgsI	TTSAA	3 cut(s)	197, 248, 608
AhdI	GACNNNNNGTC	1 cut(s)	622
AjiI	CACGTC	1 cut(s)	622
AjnI	CCWGG	3 cut(s)	292, 330, 546
AluBI	AGCT	6 cut(s)	171, 355, 443, 467, 586, 738
AluI	AGCT	6 cut(s)	171, 355, 443, 467, 586, 738
Alw21I	GWGCWC	2 cut(s)	639, 740
AlwI	GGATC	3 cut(s)	218, 259, 676
Ama87I	CYCGRG	1 cut(s)	739
AoxI	GGCC	3 cut(s)	114, 148, 601
ApeKI	GCWGC	1 cut(s)	440
ApoI	RAATTY	1 cut(s)	281
Asp700I	GAANNNNTTC	1 cut(s)	748
AspS9I	GGNCC	1 cut(s)	114
AsuHPI	GGTGA	3 cut(s)	88, 652, 700
AvaI	CYCGRG	1 cut(s)	739
BanII	GRGCYC	1 cut(s)	740
BbsI	GAAGAC	2 cut(s)	255, 741
Bbv12I	GWGCWC	2 cut(s)	639, 740
BbvI	GCAGC	1 cut(s)	452
BccI	CCATC	2 cut(s)	103, 416
BciT130I	CCWGG	3 cut(s)	294, 332, 548
BfaI	CTAG	2 cut(s)	45, 378
BisI	GCNGC	1 cut(s)	441
BlsI	GCNGC	1 cut(s)	442
Bme1390I	CCNGG	3 cut(s)	294, 332, 548
BmeRI	GACNNNNNGTC	1 cut(s)	622
BmeT110I	CYCGRG	1 cut(s)	739
BmgBI	CACGTC	1 cut(s)	622
BmgT120I	GGNCC	1 cut(s)	114
BmiI	GGNNCC	1 cut(s)	115
BmrFI	CCNGG	3 cut(s)	294, 332, 548
BmsI	GCATC	2 cut(s)	18, 655
BpiI	GAAGAC	2 cut(s)	255, 741
BpmI	CTGGAG	2 cut(s)	111, 314
BsaHI	GRCGYC	1 cut(s)	252
BsaJI	CCNNGG	2 cut(s)	293, 546
BsaWI	WCCGGW	1 cut(s)	649
BsaXI	ACNNNNNCTCC	2 cut(s)	167, 197
Bsc4I	CCNNNNNNNGG	2 cut(s)	146, 516
Bse1I	ACTGG	1 cut(s)	458
BseBI	CCWGG	3 cut(s)	294, 332, 548
BseDI	CCNNGG	2 cut(s)	293, 546
BseGI	GGATG	4 cut(s)	114, 244, 646, 706
BseLI	CCNNNNNNNGG	2 cut(s)	146, 516
BseMII	CTCAG	2 cut(s)	342, 575
BseNI	ACTGG	1 cut(s)	458
BseXI	GCAGC	1 cut(s)	452
BsgI	GTGCAG	1 cut(s)	459
Bsh1236I	CGCG	1 cut(s)	716
BshFI	GGCC	3 cut(s)	116, 150, 603
BsiHKAI	GWGCWC	2 cut(s)	639, 740
BsiHKCI	CYCGRG	1 cut(s)	739
BsiSI	CCGG	2 cut(s)	650, 698
BslI	CCNNNNNNNGG	2 cut(s)	146, 516
BsnI	GGCC	3 cut(s)	116, 150, 603
BsoBI	CYCGRG	1 cut(s)	739
Bsp1286I	GDGCHC	2 cut(s)	639, 740
Bsp1407I	TGTACA	1 cut(s)	654
Bsp143I	GATC	3 cut(s)	223, 264, 668
BspACI	CCGC	1 cut(s)	716
BspANI	GGCC	3 cut(s)	116, 150, 603
BspCNI	CTCAG	2 cut(s)	343, 574
BspFNI	CGCG	1 cut(s)	716
BspHI	TCATGA	1 cut(s)	367
BspLI	GGNNCC	1 cut(s)	115
BspPI	GGATC	3 cut(s)	218, 259, 676
BspQI	GCTCTTC	1 cut(s)	59
BsrGI	TGTACA	1 cut(s)	654
BsrI	ACTGG	1 cut(s)	458
BssECI	CCNNGG	2 cut(s)	293, 546
BssMI	GATC	3 cut(s)	223, 264, 668
BssNI	GRCGYC	1 cut(s)	252
Bst2UI	CCWGG	3 cut(s)	294, 332, 548
Bst4CI	ACNGT	2 cut(s)	323, 488
Bst6I	CTCTTC	1 cut(s)	59
BstACI	GRCGYC	1 cut(s)	252
BstAUI	TGTACA	1 cut(s)	654
BstC8I	GCNNGC	3 cut(s)	118, 122, 152
BstDEI	CTNAG	3 cut(s)	261, 351, 561
BstF5I	GGATG	4 cut(s)	114, 244, 646, 706
BstFNI	CGCG	1 cut(s)	716
BstKTI	GATC	3 cut(s)	226, 267, 671
BstMBI	GATC	3 cut(s)	223, 264, 668
BstMWI	GCNNNNNNNGC	1 cut(s)	643
BstNI	CCWGG	3 cut(s)	294, 332, 548
BstNSI	RCATGY	1 cut(s)	124
BstSCI	CCNGG	3 cut(s)	292, 330, 546
BstUI	CGCG	1 cut(s)	716
BstV1I	GCAGC	1 cut(s)	452
BstV2I	GAAGAC	2 cut(s)	255, 741
BstX2I	RGATCY	2 cut(s)	264, 668
BstYI	RGATCY	2 cut(s)	264, 668
BsuRI	GGCC	3 cut(s)	116, 150, 603
BtrI	CACGTC	1 cut(s)	622
BtsCI	GGATG	4 cut(s)	114, 244, 646, 706
BtsIMutI	CAGTG	2 cut(s)	328, 493
Cac8I	GCNNGC	3 cut(s)	118, 122, 152
CciI	TCATGA	1 cut(s)	367
Cfr13I	GGNCC	1 cut(s)	114
CseI	GACGC	1 cut(s)	260
Csp6I	GTAC	2 cut(s)	446, 655
CviAII	CATG	2 cut(s)	121, 368
CviQI	GTAC	2 cut(s)	446, 655
DdeI	CTNAG	3 cut(s)	261, 351, 561
DpnI	GATC	3 cut(s)	225, 266, 670
DpnII	GATC	3 cut(s)	223, 264, 668
DriI	GACNNNNNGTC	1 cut(s)	622
Eam1104I	CTCTTC	1 cut(s)	59
Eam1105I	GACNNNNNGTC	1 cut(s)	622
EarI	CTCTTC	1 cut(s)	59
Ecl136II	GAGCTC	1 cut(s)	738
Eco147I	AGGCCT	1 cut(s)	603
Eco24I	GRGCYC	1 cut(s)	740
Eco53kI	GAGCTC	1 cut(s)	738
Eco57I	CTGAAG	1 cut(s)	626
Eco88I	CYCGRG	1 cut(s)	739
EcoICRI	GAGCTC	1 cut(s)	738
EcoRII	CCWGG	3 cut(s)	292, 330, 546
EcoT38I	GRGCYC	1 cut(s)	740
FaeI	CATG	2 cut(s)	124, 371
FaiI	YATR	6 cut(s)	122, 166, 369, 498, 675, 734
FatI	CATG	2 cut(s)	120, 367
Fnu4HI	GCNGC	1 cut(s)	441
FokI	GGATG	4 cut(s)	121, 251, 633, 713
FriOI	GRGCYC	1 cut(s)	740
Fsp4HI	GCNGC	1 cut(s)	441
FspBI	CTAG	2 cut(s)	45, 378
GluI	GCNGC	1 cut(s)	441
GsuI	CTGGAG	2 cut(s)	111, 314
HaeIII	GGCC	3 cut(s)	116, 150, 603
HapII	CCGG	2 cut(s)	650, 698
HgaI	GACGC	1 cut(s)	260
Hin1I	GRCGYC	1 cut(s)	252
Hin1II	CATG	2 cut(s)	124, 371
HinfI	GANTC	6 cut(s)	41, 79, 311, 327, 371, 491
HpaII	CCGG	2 cut(s)	650, 698
HphI	GGTGA	3 cut(s)	88, 652, 700
Hpy166II	GTNNAC	3 cut(s)	319, 326, 655
Hpy188I	TCNGA	2 cut(s)	352, 757
Hpy188III	TCNNGA	5 cut(s)	45, 268, 368, 396, 741
Hpy8I	GTNNAC	3 cut(s)	319, 326, 655
Hpy99I	CGWCG	1 cut(s)	623
HpyAV	CCTTC	6 cut(s)	265, 292, 403, 593, 650, 659
HpyCH4III	ACNGT	2 cut(s)	323, 488
HpyCH4IV	ACGT	1 cut(s)	621
HpyCH4V	TGCA	4 cut(s)	31, 161, 440, 538
HpyF10VI	GCNNNNNNNGC	1 cut(s)	643
HpyF3I	CTNAG	3 cut(s)	261, 351, 561
HpySE526I	ACGT	1 cut(s)	621
Hsp92I	GRCGYC	1 cut(s)	252
Hsp92II	CATG	2 cut(s)	124, 371
Kzo9I	GATC	3 cut(s)	223, 264, 668
LguI	GCTCTTC	1 cut(s)	59
LmnI	GCTCC	1 cut(s)	634
Lsp1109I	GCAGC	1 cut(s)	452
LweI	GCATC	2 cut(s)	18, 655
MaeI	CTAG	2 cut(s)	45, 378
MaeII	ACGT	1 cut(s)	621
MaeIII	GTNAC	2 cut(s)	610, 688
MalI	GATC	3 cut(s)	225, 266, 670
MboI	GATC	3 cut(s)	223, 264, 668
MboII	GAAGA	5 cut(s)	76, 128, 260, 741, 744
MfeI	CAATTG	2 cut(s)	156, 336
MflI	RGATCY	2 cut(s)	264, 668
MhlI	GDGCHC	2 cut(s)	639, 740
MluCI	AATT	4 cut(s)	156, 210, 281, 336
MlyI	GAGTC	2 cut(s)	50, 321
MnlI	CCTC	5 cut(s)	86, 238, 393, 472, 568
MroXI	GAANNNNTTC	1 cut(s)	748
MseI	TTAA	5 cut(s)	279, 431, 570, 684, 728
MslI	CAYNNNNRTG	1 cut(s)	686
MspA1I	CMGCKG	1 cut(s)	443
MspI	CCGG	2 cut(s)	650, 698
MspR9I	CCNGG	3 cut(s)	294, 332, 548
MunI	CAATTG	2 cut(s)	156, 336
MvaI	CCWGG	3 cut(s)	294, 332, 548
MvnI	CGCG	1 cut(s)	716
MwoI	GCNNNNNNNGC	1 cut(s)	643
NdeII	GATC	3 cut(s)	223, 264, 668
NlaIII	CATG	2 cut(s)	124, 371
NlaIV	GGNNCC	1 cut(s)	115
NmuCI	GTSAC	2 cut(s)	610, 688
NspI	RCATGY	1 cut(s)	124
PaeI	GCATGC	1 cut(s)	124
PagI	TCATGA	1 cut(s)	367
PceI	AGGCCT	1 cut(s)	603
PciSI	GCTCTTC	1 cut(s)	59
PdmI	GAANNNNTTC	1 cut(s)	748
PfeI	GAWTC	4 cut(s)	79, 311, 371, 491
PkrI	GCNGC	1 cut(s)	442
PleI	GAGTC	2 cut(s)	49, 321
PpsI	GAGTC	2 cut(s)	49, 321
Psp124BI	GAGCTC	1 cut(s)	740
Psp6I	CCWGG	3 cut(s)	292, 330, 546
PspGI	CCWGG	3 cut(s)	292, 330, 546
PspN4I	GGNNCC	1 cut(s)	115
PspPI	GGNCC	1 cut(s)	114
PsrI	GAACNNNNNNTAC	4 cut(s)	396, 428, 438, 470
PsuI	RGATCY	2 cut(s)	264, 668
PvuII	CAGCTG	1 cut(s)	443
RsaI	GTAC	2 cut(s)	447, 656
RsaNI	GTAC	2 cut(s)	446, 655
RseI	CAYNNNNRTG	1 cut(s)	686
SacI	GAGCTC	1 cut(s)	740
SapI	GCTCTTC	1 cut(s)	59
SaqAI	TTAA	5 cut(s)	279, 431, 570, 684, 728
SatI	GCNGC	1 cut(s)	441
Sau3AI	GATC	3 cut(s)	223, 264, 668
Sau96I	GGNCC	1 cut(s)	114
SchI	GAGTC	2 cut(s)	50, 321
ScrFI	CCNGG	3 cut(s)	294, 332, 548
SduI	GDGCHC	2 cut(s)	639, 740
SfaNI	GCATC	2 cut(s)	18, 655
SmiMI	CAYNNNNRTG	1 cut(s)	686
SphI	GCATGC	1 cut(s)	124
Sse9I	AATT	4 cut(s)	156, 210, 281, 336
SseBI	AGGCCT	1 cut(s)	603
SsiI	CCGC	1 cut(s)	716
SspMI	CTAG	2 cut(s)	45, 378
SstI	GAGCTC	1 cut(s)	740
StuI	AGGCCT	1 cut(s)	603
StyD4I	CCNGG	3 cut(s)	292, 330, 546
TaaI	ACNGT	2 cut(s)	323, 488
TaiI	ACGT	1 cut(s)	624
TasI	AATT	4 cut(s)	156, 210, 281, 336
TatI	WGTACW	2 cut(s)	445, 654
TfiI	GAWTC	4 cut(s)	79, 311, 371, 491
Tru1I	TTAA	5 cut(s)	279, 431, 570, 684, 728
Tru9I	TTAA	5 cut(s)	279, 431, 570, 684, 728
TscAI	CASTG	2 cut(s)	328, 493
TseFI	GTSAC	2 cut(s)	610, 688
TseI	GCWGC	1 cut(s)	440
Tsp45I	GTSAC	2 cut(s)	610, 688
TspDTI	ATGAA	1 cut(s)	356
TspGWI	ACGGA	2 cut(s)	304, 629
TspRI	CASTG	2 cut(s)	328, 493
XapI	RAATTY	1 cut(s)	281
XbaI	TCTAGA	1 cut(s)	44
XceI	RCATGY	1 cut(s)	124
XmnI	GAANNNNTTC	1 cut(s)	748
XspI	CTAG	2 cut(s)	45, 378

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.