MD09G1006700.v1.1

Belongs to the DHHC palmitoyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008943
Physical Location & Seq
Reverse (-)
487224 .. 490996
3773 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1006700.v1.1.491

Sequence Viewer

Length: 1338 bp
ATGTACGTGGTGCCTCCACCGCAGCGATCGGATCCGGCTTCCGGATCCGCCGGCGCCGCCGATGATTTGCGAGTCTACCAGAATTGGAAGGGCAGCAATATATTTTTTTTTCAGGGGAGGTTTGTTTTCGGACCAGATGTAAGATCGTTGGGGCTGACCATATTTCTGATTGTTGCTCCTGTCACAATATTCTGCATCTTTGTTGCCAGAAAGTTGATGGATCATTACTCCCATCACTTGGGAATATTCATCATGGCTGTCGCTGTTGTATTCACAGTTTATGATTTAGTTCTTCTCCTGCTTACCTCTGGAAGAGATCCTGGTATAATTCCCCGTAACACACACCCCCCAGAGCCTGAAGGATTTGATGGGAATATAGAAGGTGGGGCTGGACAAACTCCACAGTTGCGCTTGCCTCGCATCAAAGAAGTTGAGGTGAATGGAATCTCTGTCAAGATCAAGTACTGCGATACTTGTATGCTTTATAGACCTCCTCGCTGCTCCCACTGTTCAATATGCAACAACTGTGTAGAAAGATTCGATCATCATTGTCCCTGGGTTGGGCAGTGTATTGGTCTGAGGAACTATCGATTCTTCTTCATGTTTGTCTTCAACACAACTCTTCTTTGTATATATGTTTTTGCATTCTGCTGGGTGTACGTTAGAAGGATCATGGGAGCTGAGGAGACAACGATTTGGAAGGCAATGATTAAAACTCCAGCATCTATTGTGCTAATAATTTACACTTTCATATCGATGTGGTTTGTTGGTGGCCTTACTGCCTTCCATTTGTATCTCATCAGTACAAATCAGACTACATATGAGAATTTCCGGTACCGATATGATAGGCGAGCCAACCCCTATAACAAGGGAGTTGTCGACAATTTCAAGGAGATATTTTGTACCGCCATTCCCGAGTCTAAGAACCATTTCAGGGACAAGGTGCCAAGGGAGCCGGCGTTACCTCCTCGATCAGGGGGTGGGGGTTTTATGAGCCCAAATATGGGAAAAGCTGTGGAGGACATTGAAATGGGCAGAAAAACCGTGTGGGGTGACATGAGTGCTGGGGGAGATAATTACGAAGGGCAGCATAACGGCAATGATCGCGTGAATGTAAAAGATGGTGAATTAAGTGAAGTATCCCCAGATATAAGGACGACCGTAGATGAAACTGTGGATCGTGCTGGAATGCATCCCAGGCGAGCCAGCTGGGGAAGGAAAAGTGGAAGTTGGGAAATGTCCCCAGAAGTTCTTGCCTTGGCAGCTAGAGTCGGGGAACCAAACCGCATGGGCGGGAGTAGCAGCGGTGGCCTAACGAACGAGAACCGACATACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04391 map04391
Pfam Domains
Protein Families
DHHC

Protein Analysis

446

Amino Acids

50.06

Weight (kDa)

8.53

Isoelectric Point (pI)

37.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHHC PF01529 152 - 278 8.7e-40 DHHC palmitoyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 834
AccB1I GGYRCC 4 cut(s) 10, 53, 834, 943
AccB7I CCANNNNNTGG 1 cut(s) 238
AccI GTMKAC 2 cut(s) 75, 879
AccII CGCG 1 cut(s) 1107
AccIII TCCGGA 1 cut(s) 41
AciI CCGC 7 cut(s) 20, 48, 57, 906, 1285, 1293, 1305
AclWI GGATC 8 cut(s) 26, 39, 39, 52, 228, 311, 677, 1185
AcsI RAATTY 1 cut(s) 826
AcuI CTGAAG 1 cut(s) 378
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 6 cut(s) 5, 464, 659, 805, 836, 904
AfiI CCNNNNNNNGG 8 cut(s) 41, 238, 560, 561, 934, 974, 1003, 1004
AgsI TTSAA 4 cut(s) 513, 613, 889, 1028
AjnI CCWGG 3 cut(s) 319, 554, 1196
AluBI AGCT 4 cut(s) 680, 1013, 1209, 1265
AluI AGCT 4 cut(s) 680, 1013, 1209, 1265
Alw26I GTCTC 1 cut(s) 680
AlwI GGATC 8 cut(s) 26, 39, 39, 52, 228, 311, 677, 1185
AlwNI CAGNNNCTG 1 cut(s) 356
Ama87I CYCGRG 1 cut(s) 914
Aor13HI TCCGGA 1 cut(s) 41
AoxI GGCC 2 cut(s) 772, 1309
ApeKI GCWGC 6 cut(s) 22, 93, 498, 1087, 1262, 1302
ApoI RAATTY 1 cut(s) 826
Asp700I GAANNNNTTC 1 cut(s) 929
Asp718I GGTACC 1 cut(s) 834
AspLEI GCGC 2 cut(s) 56, 411
AspS9I GGNCC 1 cut(s) 131
AsuHPI GGTGA 3 cut(s) 448, 1064, 1136
AvaI CYCGRG 1 cut(s) 914
AvaII GGWCC 1 cut(s) 131
BamHI GGATCC 2 cut(s) 31, 44
BanI GGYRCC 4 cut(s) 10, 53, 834, 943
BanII GRGCYC 1 cut(s) 998
BbsI GAAGAC 1 cut(s) 601
BbvCI CCTCAGC 1 cut(s) 681
BbvI GCAGC 6 cut(s) 34, 105, 485, 1099, 1274, 1314
BccI CCATC 4 cut(s) 211, 240, 362, 1115
BceAI ACGGC 1 cut(s) 1111
BciT130I CCWGG 3 cut(s) 321, 556, 1198
BciVI GTATCC 1 cut(s) 1150
BcoDI GTCTC 1 cut(s) 680
BfaI CTAG 1 cut(s) 1266
BfoI RGCGCY 1 cut(s) 57
BfuI GTATCC 1 cut(s) 1150
BisI GCNGC 7 cut(s) 23, 57, 94, 499, 1088, 1263, 1303
BlsI GCNGC 7 cut(s) 24, 58, 95, 500, 1089, 1264, 1304
BmcAI AGTACT 1 cut(s) 464
Bme1390I CCNGG 3 cut(s) 321, 556, 1198
Bme18I GGWCC 1 cut(s) 131
BmeT110I CYCGRG 1 cut(s) 914
BmgT120I GGNCC 1 cut(s) 131
BmiI GGNNCC 8 cut(s) 12, 33, 46, 55, 836, 945, 954, 1278
BmrFI CCNGG 3 cut(s) 321, 556, 1198
BmsI GCATC 4 cut(s) 204, 429, 731, 1201
BpiI GAAGAC 1 cut(s) 601
BpmI CTGGAG 1 cut(s) 702
Bpu10I CCTNAGC 1 cut(s) 681
Bsa29I ATCGAT 2 cut(s) 589, 755
BsaAI YACGTR 1 cut(s) 7
BsaHI GRCGYC 1 cut(s) 54
BsaJI CCNNGG 5 cut(s) 554, 555, 947, 1196, 1257
BsaWI WCCGGW 2 cut(s) 41, 831
BsaXI ACNNNNNCTCC 2 cut(s) 944, 974
Bsc4I CCNNNNNNNGG 8 cut(s) 41, 238, 560, 561, 934, 974, 1003, 1004
Bse118I RCCGGY 2 cut(s) 50, 955
Bse3DI GCAATG 2 cut(s) 711, 1105
BseAI TCCGGA 1 cut(s) 41
BseBI CCWGG 3 cut(s) 321, 556, 1198
BseCI ATCGAT 2 cut(s) 589, 755
BseDI CCNNGG 5 cut(s) 554, 555, 947, 1196, 1257
BseGI GGATG 1 cut(s) 1192
BseLI CCNNNNNNNGG 8 cut(s) 41, 238, 560, 561, 934, 974, 1003, 1004
BseMI GCAATG 2 cut(s) 711, 1105
BseMII CTCAG 2 cut(s) 569, 672
BseRI GAGGAG 3 cut(s) 483, 698, 957
BseXI GCAGC 6 cut(s) 34, 105, 485, 1099, 1274, 1314
BseYI CCCAGC 3 cut(s) 651, 1064, 1209
Bsh1236I CGCG 1 cut(s) 1107
Bsh1285I CGRYCG 2 cut(s) 29, 1161
BshFI GGCC 2 cut(s) 774, 1311
BshNI GGYRCC 4 cut(s) 10, 53, 834, 943
BshVI ATCGAT 2 cut(s) 589, 755
BsiEI CGRYCG 2 cut(s) 29, 1161
BsiHKCI CYCGRG 1 cut(s) 914
BsiSI CCGG 5 cut(s) 35, 42, 51, 832, 956
BslFI GGGAC 3 cut(s) 537, 950, 1225
BslI CCNNNNNNNGG 8 cut(s) 41, 238, 560, 561, 934, 974, 1003, 1004
BsmAI GTCTC 1 cut(s) 680
BsmFI GGGAC 3 cut(s) 537, 950, 1225
BsmI GAATGC 2 cut(s) 644, 1194
BsnI GGCC 2 cut(s) 774, 1311
BsoBI CYCGRG 1 cut(s) 914
Bsp1286I GDGCHC 1 cut(s) 998
Bsp13I TCCGGA 1 cut(s) 41
BspACI CCGC 7 cut(s) 20, 48, 57, 906, 1285, 1293, 1305
BspANI GGCC 2 cut(s) 774, 1311
BspCNI CTCAG 2 cut(s) 570, 673
BspDI ATCGAT 2 cut(s) 589, 755
BspEI TCCGGA 1 cut(s) 41
BspFNI CGCG 1 cut(s) 1107
BspLI GGNNCC 8 cut(s) 12, 33, 46, 55, 836, 945, 954, 1278
BspPI GGATC 8 cut(s) 26, 39, 39, 52, 228, 311, 677, 1185
BspT107I GGYRCC 4 cut(s) 10, 53, 834, 943
BsrDI GCAATG 2 cut(s) 711, 1105
BsrFI RCCGGY 2 cut(s) 50, 955
BssAI RCCGGY 2 cut(s) 50, 955
BssECI CCNNGG 5 cut(s) 554, 555, 947, 1196, 1257
BssNI GRCGYC 1 cut(s) 54
BssT1I CCWWGG 2 cut(s) 947, 1257
Bst2UI CCWGG 3 cut(s) 321, 556, 1198
Bst4CI ACNGT 7 cut(s) 277, 405, 509, 527, 1045, 1162, 1174
Bst6I CTCTTC 2 cut(s) 307, 627
BstACI GRCGYC 1 cut(s) 54
BstBAI YACGTR 1 cut(s) 7
BstC8I GCNNGC 6 cut(s) 52, 413, 852, 957, 1203, 1207
BstDEI CTNAG 3 cut(s) 578, 681, 921
BstENI CCTNNNNNAGG 1 cut(s) 972
BstF5I GGATG 1 cut(s) 1192
BstFNI CGCG 1 cut(s) 1107
BstH2I RGCGCY 1 cut(s) 57
BstHHI GCGC 2 cut(s) 56, 411
BstMAI GTCTC 1 cut(s) 680
BstMCI CGRYCG 2 cut(s) 29, 1161
BstMWI GCNNNNNNNGC 9 cut(s) 19, 56, 417, 952, 1104, 1198, 1262, 1299, 1308
BstNI CCWGG 3 cut(s) 321, 556, 1198
BstSCI CCNGG 3 cut(s) 319, 554, 1196
BstUI CGCG 1 cut(s) 1107
BstV1I GCAGC 6 cut(s) 34, 105, 485, 1099, 1274, 1314
BstV2I GAAGAC 1 cut(s) 601
BstX2I RGATCY 3 cut(s) 31, 44, 316
BstYI RGATCY 3 cut(s) 31, 44, 316
Bsu15I ATCGAT 2 cut(s) 589, 755
BsuI GTATCC 1 cut(s) 1150
BsuRI GGCC 2 cut(s) 774, 1311
BsuTUI ATCGAT 2 cut(s) 589, 755
BtsCI GGATG 1 cut(s) 1192
BtsI GCAGTG 1 cut(s) 572
BtsIMutI CAGTG 2 cut(s) 505, 572
Cac8I GCNNGC 6 cut(s) 52, 413, 852, 957, 1203, 1207
CaiI CAGNNNCTG 1 cut(s) 356
CfoI GCGC 2 cut(s) 56, 411
Cfr10I RCCGGY 2 cut(s) 50, 955
Cfr13I GGNCC 1 cut(s) 131
ClaI ATCGAT 2 cut(s) 589, 755
Csp6I GTAC 6 cut(s) 4, 463, 658, 804, 835, 903
CviAII CATG 5 cut(s) 253, 601, 673, 1057, 1288
CviQI GTAC 6 cut(s) 4, 463, 658, 804, 835, 903
DdeI CTNAG 3 cut(s) 578, 681, 921
DinI GGCGCC 1 cut(s) 55
Eam1104I CTCTTC 2 cut(s) 307, 627
EarI CTCTTC 2 cut(s) 307, 627
EciI GGCGGA 1 cut(s) 37
Eco130I CCWWGG 2 cut(s) 947, 1257
Eco24I GRGCYC 1 cut(s) 998
Eco47I GGWCC 1 cut(s) 131
Eco57I CTGAAG 1 cut(s) 378
Eco88I CYCGRG 1 cut(s) 914
EcoNI CCTNNNNNAGG 1 cut(s) 972
EcoRII CCWGG 3 cut(s) 319, 554, 1196
EcoT14I CCWWGG 2 cut(s) 947, 1257
EcoT22I ATGCAT 1 cut(s) 1194
EcoT38I GRGCYC 1 cut(s) 998
EgeI GGCGCC 1 cut(s) 55
EheI GGCGCC 1 cut(s) 55
ErhI CCWWGG 2 cut(s) 947, 1257
FaeI CATG 5 cut(s) 256, 604, 676, 1060, 1291
FaqI GGGAC 3 cut(s) 537, 950, 1225
FatI CATG 5 cut(s) 252, 600, 672, 1056, 1287
FauI CCCGC 1 cut(s) 1286
FauNDI CATATG 1 cut(s) 820
FblI GTMKAC 2 cut(s) 75, 879
Fnu4HI GCNGC 7 cut(s) 23, 57, 94, 499, 1088, 1263, 1303
FokI GGATG 1 cut(s) 1179
FriOI GRGCYC 1 cut(s) 998
Fsp4HI GCNGC 7 cut(s) 23, 57, 94, 499, 1088, 1263, 1303
FspBI CTAG 1 cut(s) 1266
GlaI GCGC 2 cut(s) 55, 410
GluI GCNGC 7 cut(s) 23, 57, 94, 499, 1088, 1263, 1303
GsaI CCCAGC 3 cut(s) 655, 1068, 1213
GsuI CTGGAG 1 cut(s) 702
HaeII RGCGCY 1 cut(s) 57
HaeIII GGCC 2 cut(s) 774, 1311
HapII CCGG 5 cut(s) 35, 42, 51, 832, 956
HhaI GCGC 2 cut(s) 56, 411
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 5 cut(s) 256, 604, 676, 1060, 1291
Hin6I GCGC 2 cut(s) 54, 409
HinP1I GCGC 2 cut(s) 54, 409
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HinfI GANTC 6 cut(s) 72, 444, 537, 591, 917, 1269
HpaII CCGG 5 cut(s) 35, 42, 51, 832, 956
HphI GGTGA 3 cut(s) 448, 1064, 1136
Hpy166II GTNNAC 3 cut(s) 76, 658, 880
Hpy188I TCNGA 5 cut(s) 31, 131, 168, 579, 813
Hpy188III TCNNGA 4 cut(s) 42, 309, 454, 914
Hpy8I GTNNAC 3 cut(s) 76, 658, 880
HpyAV CCTTC 8 cut(s) 82, 353, 374, 660, 694, 793, 1076, 1209
HpyCH4III ACNGT 7 cut(s) 277, 405, 509, 527, 1045, 1162, 1174
HpyCH4IV ACGT 2 cut(s) 6, 660
HpyCH4V TGCA 4 cut(s) 195, 519, 644, 1192
HpyF10VI GCNNNNNNNGC 9 cut(s) 19, 56, 417, 952, 1104, 1198, 1262, 1299, 1308
HpyF3I CTNAG 3 cut(s) 578, 681, 921
HpySE526I ACGT 2 cut(s) 6, 660
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 5 cut(s) 256, 604, 676, 1060, 1291
HspAI GCGC 2 cut(s) 54, 409
KasI GGCGCC 1 cut(s) 53
Kpn2I TCCGGA 1 cut(s) 41
KpnI GGTACC 1 cut(s) 838
KroI GCCGGC 2 cut(s) 50, 955
KroNI GCCGGC 2 cut(s) 52, 957
LmnI GCTCC 4 cut(s) 181, 506, 677, 952
Lsp1109I GCAGC 6 cut(s) 34, 105, 485, 1099, 1274, 1314
LweI GCATC 4 cut(s) 204, 429, 731, 1201
MaeI CTAG 1 cut(s) 1266
MaeII ACGT 2 cut(s) 6, 660
MaeIII GTNAC 4 cut(s) 181, 335, 960, 1052
MboII GAAGA 6 cut(s) 284, 324, 586, 589, 601, 614
MflI RGATCY 3 cut(s) 31, 44, 316
MhlI GDGCHC 1 cut(s) 998
MluCI AATT 7 cut(s) 82, 327, 738, 826, 883, 1075, 1127
Mly113I GGCGCC 1 cut(s) 54
MlyI GAGTC 3 cut(s) 81, 926, 1278
Mph1103I ATGCAT 1 cut(s) 1194
MreI CGCCGGCG 1 cut(s) 50
MroI TCCGGA 1 cut(s) 41
MroNI GCCGGC 2 cut(s) 50, 955
MroXI GAANNNNTTC 1 cut(s) 929
MseI TTAA 2 cut(s) 711, 1130
MslI CAYNNNNRTG 2 cut(s) 755, 1028
MspA1I CMGCKG 2 cut(s) 1209, 1305
MspI CCGG 5 cut(s) 35, 42, 51, 832, 956
MspR9I CCNGG 3 cut(s) 321, 556, 1198
Mva1269I GAATGC 2 cut(s) 644, 1194
MvaI CCWGG 3 cut(s) 321, 556, 1198
MvnI CGCG 1 cut(s) 1107
MwoI GCNNNNNNNGC 9 cut(s) 19, 56, 417, 952, 1104, 1198, 1262, 1299, 1308
NaeI GCCGGC 2 cut(s) 52, 957
NarI GGCGCC 1 cut(s) 54
NdeI CATATG 1 cut(s) 820
NgoMIV GCCGGC 2 cut(s) 50, 955
NlaIII CATG 5 cut(s) 256, 604, 676, 1060, 1291
NlaIV GGNNCC 8 cut(s) 12, 33, 46, 55, 836, 945, 954, 1278
NmuCI GTSAC 2 cut(s) 181, 1052
NsiI ATGCAT 1 cut(s) 1194
PasI CCCWGGG 1 cut(s) 555
PctI GAATGC 2 cut(s) 644, 1194
PdiI GCCGGC 2 cut(s) 52, 957
PdmI GAANNNNTTC 1 cut(s) 929
PfeI GAWTC 3 cut(s) 444, 537, 591
PflMI CCANNNNNTGG 1 cut(s) 238
PkrI GCNGC 7 cut(s) 24, 58, 95, 500, 1089, 1264, 1304
Ple19I CGATCG 1 cut(s) 29
PleI GAGTC 3 cut(s) 80, 925, 1277
PluTI GGCGCC 1 cut(s) 57
PpsI GAGTC 3 cut(s) 80, 925, 1277
Ppu21I YACGTR 1 cut(s) 7
Psp6I CCWGG 3 cut(s) 319, 554, 1196
PspFI CCCAGC 3 cut(s) 651, 1064, 1209
PspGI CCWGG 3 cut(s) 319, 554, 1196
PspN4I GGNNCC 8 cut(s) 12, 33, 46, 55, 836, 945, 954, 1278
PspPI GGNCC 1 cut(s) 131
PstNI CAGNNNCTG 1 cut(s) 356
PsuI RGATCY 3 cut(s) 31, 44, 316
PvuI CGATCG 1 cut(s) 29
PvuII CAGCTG 1 cut(s) 1209
RsaI GTAC 6 cut(s) 5, 464, 659, 805, 836, 904
RsaNI GTAC 6 cut(s) 4, 463, 658, 804, 835, 903
RseI CAYNNNNRTG 2 cut(s) 755, 1028
SalI GTCGAC 1 cut(s) 878
SaqAI TTAA 2 cut(s) 711, 1130
SatI GCNGC 7 cut(s) 23, 57, 94, 499, 1088, 1263, 1303
Sau96I GGNCC 1 cut(s) 131
ScaI AGTACT 1 cut(s) 464
SchI GAGTC 3 cut(s) 81, 926, 1278
ScrFI CCNGG 3 cut(s) 321, 556, 1198
SduI GDGCHC 1 cut(s) 998
SfaNI GCATC 4 cut(s) 204, 429, 731, 1201
SfoI GGCGCC 1 cut(s) 55
SgrAI CRCCGGYG 1 cut(s) 50
SinI GGWCC 1 cut(s) 131
SmiMI CAYNNNNRTG 2 cut(s) 755, 1028
Sse9I AATT 7 cut(s) 82, 327, 738, 826, 883, 1075, 1127
SsiI CCGC 7 cut(s) 20, 48, 57, 906, 1285, 1293, 1305
SspDI GGCGCC 1 cut(s) 53
SspI AATATT 2 cut(s) 189, 246
SspMI CTAG 1 cut(s) 1266
StyD4I CCNGG 3 cut(s) 319, 554, 1196
StyI CCWWGG 2 cut(s) 947, 1257
TaaI ACNGT 7 cut(s) 277, 405, 509, 527, 1045, 1162, 1174
TaiI ACGT 2 cut(s) 9, 663
TaqI TCGA 5 cut(s) 540, 589, 755, 879, 970
TasI AATT 7 cut(s) 82, 327, 738, 826, 883, 1075, 1127
TatI WGTACW 2 cut(s) 462, 803
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 3 cut(s) 444, 537, 591
Tru1I TTAA 2 cut(s) 711, 1130
Tru9I TTAA 2 cut(s) 711, 1130
TscAI CASTG 2 cut(s) 512, 572
TseFI GTSAC 2 cut(s) 181, 1052
TseI GCWGC 6 cut(s) 22, 93, 498, 1087, 1262, 1302
Tsp45I GTSAC 2 cut(s) 181, 1052
TspDTI ATGAA 4 cut(s) 238, 589, 739, 1182
TspRI CASTG 2 cut(s) 512, 572
Van91I CCANNNNNTGG 1 cut(s) 238
VpaK11BI GGWCC 1 cut(s) 131
XagI CCTNNNNNAGG 1 cut(s) 972
XapI RAATTY 1 cut(s) 826
XcmI CCANNNNNNNNNTGG 1 cut(s) 214
XmiI GTMKAC 2 cut(s) 75, 879
XmnI GAANNNNTTC 1 cut(s) 929
XspI CTAG 1 cut(s) 1266
ZrmI AGTACT 1 cut(s) 464
Zsp2I ATGCAT 1 cut(s) 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.