MD09G1015600.v1.1

heme protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0010327
Physical Location & Seq
Forward (+)
961297 .. 964466
3170 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1015600.v1.1.491

Sequence Viewer

Length: 945 bp
ATGCGCAGAATATTGACTGAAATGGCTGGAGGAGCGATCCACCAGTTATTGAGGAGGAAGCTTCATTCTCAATCTACTGCCTCTCCATTGTTGGCTTCTCTAATACATAAGAAAGATGATGCTGGCTCTGCTGGGGCAAGGTCCCTCAGAGCACTTGCGCTCCTTGGAGCAGGTGTTTCAGGACTGTTAAGTTTTGCAACTGTTGCATCTGCAGATGAGGCTGAACATGGATTAGAGAGCCCAAACTACCCCTGGCCTCATGCAGGAATCCTTAGCTCATATGATCATGCTTCGATTCGTCGTGGTCATCAGGTTTACACACAAGTCTGTGCATCCTGCCATTCCATGTCTTTAATATCCTACCGTGATTTGGTGGGTGTTGCTTATACCGAAGAGGAGACAAAGGCTATGGCAGCTGAGATTGAGGTAACAGATGGGCCGAACGACGAGGGTGAGTTGTTTACTCGGCCTGGTAAACTTAGTGATCGATTCCCTCAGCCATATGCAAATGAGCAGGCAGCTAGGTTTGCTAATGGTGGAGCATATCCTCCTGATTTGAGTCTTATTACAAAGGCTCGTCACAATGGTCAGAATTATGTATTTGCCCTTCTCACCGGTTACCGTGATCCTCCAGCTGGTGTTCAGATCCGAGATGGCCTGCACTATAATCCGTACTTTCCTGGGGGAGCAATTGCTATGCCTAAAATGCTTAATGATGGTGCTGTTGAGTACGAAGATGGTACTCCTGCAACTGAGGCTCAGATGGGGAAAGATGTTGTGTCATTCTTGACATGGGCCGCAGAACCAGAAATGGAAGAGAGGAAACTCATGGGATTCAAATGGATCTTTGTTCTCTCTCTGGCGCTACTTCAAGCTGCATACTACCGGCGCTTGAAGTGGTCAGTTCTCAAGTCCCGCAAGCTCGTTCTCGACGTTGTCAACTAA

Protein Analysis

315

Amino Acids

34.37

Weight (kDa)

6.56

Isoelectric Point (pI)

45.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_C1 PF02167 85 - 301 8.4e-100 Cytochrome C1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 161
Acc16I TGCGCA 1 cut(s) 5
Acc36I ACCTGC 1 cut(s) 161
AciI CCGC 2 cut(s) 798, 916
AclWI GGATC 4 cut(s) 31, 620, 640, 851
AfaI GTAC 3 cut(s) 674, 731, 742
AfiI CCNNNNNNNGG 3 cut(s) 263, 370, 635
AgeI ACCGGT 1 cut(s) 614
AgsI TTSAA 3 cut(s) 838, 872, 895
AjnI CCWGG 3 cut(s) 251, 469, 679
AluBI AGCT 7 cut(s) 61, 276, 416, 521, 635, 875, 922
AluI AGCT 7 cut(s) 61, 276, 416, 521, 635, 875, 922
Alw21I GWGCWC 1 cut(s) 154
Alw26I GTCTC 1 cut(s) 392
AlwI GGATC 4 cut(s) 31, 620, 640, 851
AoxI GGCC 5 cut(s) 254, 437, 467, 655, 795
ApeKI GCWGC 3 cut(s) 413, 518, 875
AsiGI ACCGGT 1 cut(s) 614
AspLEI GCGC 4 cut(s) 6, 160, 865, 891
AspS9I GGNCC 3 cut(s) 141, 437, 795
AsuHPI GGTGA 2 cut(s) 464, 604
AvaII GGWCC 1 cut(s) 141
BanII GRGCYC 1 cut(s) 242
BarI GAAGNNNNNNTAC 2 cut(s) 591, 623
Bbv12I GWGCWC 1 cut(s) 154
BbvCI CCTCAGC 1 cut(s) 495
BbvI GCAGC 3 cut(s) 425, 530, 862
BccI CCATC 5 cut(s) 428, 647, 710, 731, 757
BciT130I CCWGG 3 cut(s) 253, 471, 681
BclI TGATCA 1 cut(s) 283
BcoDI GTCTC 1 cut(s) 392
BfaI CTAG 1 cut(s) 522
BfmI CTRYAG 1 cut(s) 210
BfoI RGCGCY 2 cut(s) 866, 892
BfuAI ACCTGC 1 cut(s) 161
BisI GCNGC 4 cut(s) 414, 519, 798, 876
BlsI GCNGC 4 cut(s) 415, 520, 799, 877
Bme1390I CCNGG 3 cut(s) 253, 471, 681
Bme18I GGWCC 1 cut(s) 141
BmgT120I GGNCC 3 cut(s) 141, 437, 795
BmiI GGNNCC 1 cut(s) 143
BmrFI CCNGG 3 cut(s) 253, 471, 681
BmsI GCATC 3 cut(s) 109, 215, 341
BpmI CTGGAG 2 cut(s) 48, 615
Bpu10I CCTNAGC 2 cut(s) 272, 495
BpuEI CTTGAG 1 cut(s) 893
Bsa29I ATCGAT 1 cut(s) 487
BsaJI CCNNGG 3 cut(s) 163, 251, 680
BsaWI WCCGGW 1 cut(s) 614
BsaXI ACNNNNNCTCC 6 cut(s) 67, 97, 144, 159, 174, 189
Bsc4I CCNNNNNNNGG 3 cut(s) 263, 370, 635
Bse118I RCCGGY 2 cut(s) 614, 885
Bse1I ACTGG 1 cut(s) 43
BseBI CCWGG 3 cut(s) 253, 471, 681
BseCI ATCGAT 1 cut(s) 487
BseDI CCNNGG 3 cut(s) 163, 251, 680
BseGI GGATG 1 cut(s) 332
BseLI CCNNNNNNNGG 3 cut(s) 263, 370, 635
BseMII CTCAG 5 cut(s) 160, 408, 509, 744, 773
BseNI ACTGG 1 cut(s) 43
BseRI GAGGAG 3 cut(s) 45, 67, 410
BseXI GCAGC 3 cut(s) 425, 530, 862
BseYI CCCAGC 1 cut(s) 131
BsgI GTGCAG 1 cut(s) 644
BshFI GGCC 5 cut(s) 256, 439, 469, 657, 797
BshTI ACCGGT 1 cut(s) 614
BshVI ATCGAT 1 cut(s) 487
BsiHKAI GWGCWC 1 cut(s) 154
BsiSI CCGG 2 cut(s) 615, 886
BslFI GGGAC 2 cut(s) 127, 898
BslI CCNNNNNNNGG 3 cut(s) 263, 370, 635
BsmAI GTCTC 1 cut(s) 392
BsmFI GGGAC 2 cut(s) 127, 898
BsnI GGCC 5 cut(s) 256, 439, 469, 657, 797
Bsp1286I GDGCHC 2 cut(s) 154, 242
Bsp143I GATC 6 cut(s) 36, 283, 484, 625, 645, 843
BspACI CCGC 2 cut(s) 798, 916
BspANI GGCC 5 cut(s) 256, 439, 469, 657, 797
BspCNI CTCAG 5 cut(s) 159, 409, 508, 745, 772
BspDI ATCGAT 1 cut(s) 487
BspLI GGNNCC 1 cut(s) 143
BspMAI CTGCAG 1 cut(s) 214
BspMI ACCTGC 1 cut(s) 161
BspPI GGATC 4 cut(s) 31, 620, 640, 851
BsrFI RCCGGY 2 cut(s) 614, 885
BsrI ACTGG 1 cut(s) 43
BssAI RCCGGY 2 cut(s) 614, 885
BssECI CCNNGG 3 cut(s) 163, 251, 680
BssMI GATC 6 cut(s) 36, 283, 484, 625, 645, 843
BssT1I CCWWGG 1 cut(s) 163
Bst2UI CCWGG 3 cut(s) 253, 471, 681
Bst4CI ACNGT 4 cut(s) 186, 202, 365, 623
Bst6I CTCTTC 2 cut(s) 387, 810
BstAPI GCANNNNNTGC 1 cut(s) 203
BstC8I GCNNGC 4 cut(s) 124, 516, 659, 920
BstDEI CTNAG 7 cut(s) 146, 272, 417, 479, 495, 753, 759
BstEII GGTNACC 1 cut(s) 617
BstENI CCTNNNNNAGG 1 cut(s) 261
BstF5I GGATG 1 cut(s) 332
BstH2I RGCGCY 2 cut(s) 866, 892
BstHHI GCGC 4 cut(s) 6, 160, 865, 891
BstKTI GATC 6 cut(s) 39, 286, 487, 628, 648, 846
BstMAI GTCTC 1 cut(s) 392
BstMBI GATC 6 cut(s) 36, 283, 484, 625, 645, 843
BstMWI GCNNNNNNNGC 8 cut(s) 32, 128, 203, 218, 413, 527, 706, 755
BstNI CCWGG 3 cut(s) 253, 471, 681
BstPI GGTNACC 1 cut(s) 617
BstSCI CCNGG 3 cut(s) 251, 469, 679
BstSFI CTRYAG 1 cut(s) 210
BstV1I GCAGC 3 cut(s) 425, 530, 862
BstX2I RGATCY 2 cut(s) 645, 843
BstYI RGATCY 2 cut(s) 645, 843
Bsu15I ATCGAT 1 cut(s) 487
BsuRI GGCC 5 cut(s) 256, 439, 469, 657, 797
BsuTUI ATCGAT 1 cut(s) 487
BtsCI GGATG 1 cut(s) 332
BveI ACCTGC 1 cut(s) 161
Cac8I GCNNGC 4 cut(s) 124, 516, 659, 920
CfoI GCGC 4 cut(s) 6, 160, 865, 891
Cfr10I RCCGGY 2 cut(s) 614, 885
Cfr13I GGNCC 3 cut(s) 141, 437, 795
ClaI ATCGAT 1 cut(s) 487
Csp6I GTAC 3 cut(s) 673, 730, 741
CspAI ACCGGT 1 cut(s) 614
CviAII CATG 6 cut(s) 227, 260, 287, 346, 792, 829
CviQI GTAC 3 cut(s) 673, 730, 741
DdeI CTNAG 7 cut(s) 146, 272, 417, 479, 495, 753, 759
DpnI GATC 6 cut(s) 38, 285, 486, 627, 647, 845
DpnII GATC 6 cut(s) 36, 283, 484, 625, 645, 843
Eam1104I CTCTTC 2 cut(s) 387, 810
EarI CTCTTC 2 cut(s) 387, 810
Eco130I CCWWGG 1 cut(s) 163
Eco24I GRGCYC 1 cut(s) 242
Eco47I GGWCC 1 cut(s) 141
Eco91I GGTNACC 1 cut(s) 617
EcoNI CCTNNNNNAGG 1 cut(s) 261
EcoO109I RGGNCCY 1 cut(s) 141
EcoO65I GGTNACC 1 cut(s) 617
EcoRII CCWGG 3 cut(s) 251, 469, 679
EcoT14I CCWWGG 1 cut(s) 163
EcoT38I GRGCYC 1 cut(s) 242
ErhI CCWWGG 1 cut(s) 163
FaeI CATG 6 cut(s) 230, 263, 290, 349, 795, 832
FaqI GGGAC 2 cut(s) 127, 898
FatI CATG 6 cut(s) 226, 259, 286, 345, 791, 828
FauI CCCGC 1 cut(s) 923
FauNDI CATATG 2 cut(s) 280, 502
FbaI TGATCA 1 cut(s) 283
Fnu4HI GCNGC 4 cut(s) 414, 519, 798, 876
FokI GGATG 1 cut(s) 319
FriOI GRGCYC 1 cut(s) 242
Fsp4HI GCNGC 4 cut(s) 414, 519, 798, 876
FspBI CTAG 1 cut(s) 522
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 4 cut(s) 5, 159, 864, 890
GluI GCNGC 4 cut(s) 414, 519, 798, 876
GsaI CCCAGC 1 cut(s) 135
GsuI CTGGAG 2 cut(s) 48, 615
HaeII RGCGCY 2 cut(s) 866, 892
HaeIII GGCC 5 cut(s) 256, 439, 469, 657, 797
HapII CCGG 2 cut(s) 615, 886
HhaI GCGC 4 cut(s) 6, 160, 865, 891
Hin1II CATG 6 cut(s) 230, 263, 290, 349, 795, 832
Hin6I GCGC 4 cut(s) 4, 158, 863, 889
HinP1I GCGC 4 cut(s) 4, 158, 863, 889
HincII GTYRAC 1 cut(s) 940
HindII GTYRAC 1 cut(s) 940
HindIII AAGCTT 1 cut(s) 59
HinfI GANTC 5 cut(s) 267, 295, 489, 559, 834
HpaII CCGG 2 cut(s) 615, 886
HphI GGTGA 2 cut(s) 464, 604
Hpy166II GTNNAC 4 cut(s) 316, 462, 476, 940
Hpy188I TCNGA 5 cut(s) 149, 591, 645, 650, 762
Hpy188III TCNNGA 4 cut(s) 180, 551, 787, 929
Hpy8I GTNNAC 4 cut(s) 316, 462, 476, 940
Hpy99I CGWCG 3 cut(s) 303, 449, 935
HpyAV CCTTC 1 cut(s) 617
HpyCH4III ACNGT 4 cut(s) 186, 202, 365, 623
HpyCH4IV ACGT 1 cut(s) 933
HpyCH4V TGCA 9 cut(s) 197, 206, 212, 263, 332, 506, 661, 749, 878
HpyF10VI GCNNNNNNNGC 8 cut(s) 32, 128, 203, 218, 413, 527, 706, 755
HpyF3I CTNAG 7 cut(s) 146, 272, 417, 479, 495, 753, 759
HpySE526I ACGT 1 cut(s) 933
Hsp92II CATG 6 cut(s) 230, 263, 290, 349, 795, 832
HspAI GCGC 4 cut(s) 4, 158, 863, 889
Ksp22I TGATCA 1 cut(s) 283
Kzo9I GATC 6 cut(s) 36, 283, 484, 625, 645, 843
LmnI GCTCC 5 cut(s) 32, 165, 167, 539, 686
Lsp1109I GCAGC 3 cut(s) 425, 530, 862
LweI GCATC 3 cut(s) 109, 215, 341
MaeI CTAG 1 cut(s) 522
MaeII ACGT 1 cut(s) 933
MaeIII GTNAC 3 cut(s) 427, 578, 617
MalI GATC 6 cut(s) 38, 285, 486, 627, 647, 845
MboI GATC 6 cut(s) 36, 283, 484, 625, 645, 843
MboII GAAGA 3 cut(s) 404, 746, 827
MfeI CAATTG 1 cut(s) 690
MflI RGATCY 2 cut(s) 645, 843
MhlI GDGCHC 2 cut(s) 154, 242
MluCI AATT 2 cut(s) 592, 690
MlyI GAGTC 1 cut(s) 568
MseI TTAA 3 cut(s) 188, 353, 711
MspA1I CMGCKG 2 cut(s) 416, 635
MspI CCGG 2 cut(s) 615, 886
MspR9I CCNGG 3 cut(s) 253, 471, 681
MunI CAATTG 1 cut(s) 690
MvaI CCWGG 3 cut(s) 253, 471, 681
MwoI GCNNNNNNNGC 8 cut(s) 32, 128, 203, 218, 413, 527, 706, 755
NdeI CATATG 2 cut(s) 280, 502
NdeII GATC 6 cut(s) 36, 283, 484, 625, 645, 843
NlaIII CATG 6 cut(s) 230, 263, 290, 349, 795, 832
NlaIV GGNNCC 1 cut(s) 143
NmeAIII GCCGAG 1 cut(s) 445
NmuCI GTSAC 1 cut(s) 578
NsbI TGCGCA 1 cut(s) 5
PaqCI CACCTGC 1 cut(s) 161
PcsI WCGNNNNNNNCGW 1 cut(s) 930
PfeI GAWTC 4 cut(s) 267, 295, 489, 834
PflFI GACNNNGTC 1 cut(s) 935
PinAI ACCGGT 1 cut(s) 614
PkrI GCNGC 4 cut(s) 415, 520, 799, 877
PleI GAGTC 1 cut(s) 567
PpsI GAGTC 1 cut(s) 567
PpuMI RGGWCCY 1 cut(s) 141
Psp5II RGGWCCY 1 cut(s) 141
Psp6I CCWGG 3 cut(s) 251, 469, 679
PspEI GGTNACC 1 cut(s) 617
PspFI CCCAGC 1 cut(s) 131
PspGI CCWGG 3 cut(s) 251, 469, 679
PspN4I GGNNCC 1 cut(s) 143
PspPI GGNCC 3 cut(s) 141, 437, 795
PspPPI RGGWCCY 1 cut(s) 141
PstI CTGCAG 1 cut(s) 214
PsuI RGATCY 2 cut(s) 645, 843
PsyI GACNNNGTC 1 cut(s) 935
PvuII CAGCTG 2 cut(s) 416, 635
RsaI GTAC 3 cut(s) 674, 731, 742
RsaNI GTAC 3 cut(s) 673, 730, 741
SaqAI TTAA 3 cut(s) 188, 353, 711
SatI GCNGC 4 cut(s) 414, 519, 798, 876
Sau3AI GATC 6 cut(s) 36, 283, 484, 625, 645, 843
Sau96I GGNCC 3 cut(s) 141, 437, 795
SchI GAGTC 1 cut(s) 568
ScrFI CCNGG 3 cut(s) 253, 471, 681
SduI GDGCHC 2 cut(s) 154, 242
SfaNI GCATC 3 cut(s) 109, 215, 341
SfcI CTRYAG 1 cut(s) 210
SinI GGWCC 1 cut(s) 141
SmlI CTYRAG 1 cut(s) 908
SmoI CTYRAG 1 cut(s) 908
Sse9I AATT 2 cut(s) 592, 690
SsiI CCGC 2 cut(s) 798, 916
SspI AATATT 1 cut(s) 12
SspMI CTAG 1 cut(s) 522
StyD4I CCNGG 3 cut(s) 251, 469, 679
StyI CCWWGG 1 cut(s) 163
TaaI ACNGT 4 cut(s) 186, 202, 365, 623
TaiI ACGT 1 cut(s) 936
TaqI TCGA 3 cut(s) 293, 487, 930
TasI AATT 2 cut(s) 592, 690
TauI GCSGC 1 cut(s) 800
TfiI GAWTC 4 cut(s) 267, 295, 489, 834
Tru1I TTAA 3 cut(s) 188, 353, 711
Tru9I TTAA 3 cut(s) 188, 353, 711
TseFI GTSAC 1 cut(s) 578
TseI GCWGC 3 cut(s) 413, 518, 875
Tsp45I GTSAC 1 cut(s) 578
TspDTI ATGAA 1 cut(s) 53
TspGWI ACGGA 1 cut(s) 660
Tth111I GACNNNGTC 1 cut(s) 935
VpaK11BI GGWCC 1 cut(s) 141
XagI CCTNNNNNAGG 1 cut(s) 261
XcmI CCANNNNNNNNNTGG 1 cut(s) 249
XspI CTAG 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.