Rorug02G0578700

heme protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0010327
Physical Location & Seq
Reverse (-)
70209014 .. 70212984
3971 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0578700.1

Sequence Viewer

Length: 1710 bp
ATGGCAAGTATCAGTGTACCAACGGCCCAAGAATTAACAAAGCCAGATGAGGCTAAATCGAGACGAAATTCTACAGGAAAGATATTATCGTCGAACAGTGGAGAAAAAGTTCCTCATTATCTCAGGGCTTCCACTGGTTCCTGTCATGATTTTTGTAAATATGGGAGGAAAGATGCATTTGAAGAGAAGGAAAGATGCCCCATACGGGTTGTACCAAGAAGACTCTCAACTAAATCACTTGATAGCCAAAACTCAGCAGAAAGTGTAGTTTTGCCAGAGATCAAGAATATATCAGTGATCAAGCTCAATCATTCACCCGAGTCCACAATCCTCTCACCTGATACATGTACTACCATCGCCAAGCAGGCCAAGCAGCAACTGCCAAAAAGGTCAGCTGACAGAAAAACTGCTGTTGGGAGTGAGCTTCTTGCTGAGAGGAAGAAACCATCATTGGTGAAGTTCAAAACGTCATCCAATTCAAAACCTCCTGTATCTGCTGTGCCCAAAACTGTGAAGCAGGAAGTTTCGTCATCTCCTGAAAAGCTGAAAGTCTCTTCAAAGAAAGGTTGGACAAAACTTAAGGAGAAAGACTTGTCTACAAAACATGTCACTTCTTCGAAGCCAAAATCTCTGACCACGAAGGAATTGTCATCTCCTGATACTTCAGGAGGTTCAGAGGGGATAAGAAACAGTAATTCAAAGGTAGGCCAGAGGACAGTCACCTCTCTGAAGCCAAAATCTTTGGCTGCGAAGCAAATTTCTTCTCTTGACTCTTCAGGAGGCTTAAATGGCGGTAGAAAAAGTGAGGCGAAGATAGGCAAGAGGGCAGGAACGTCCTTTGTTGCTTTAAAGAAAGTATTGGTGCCCCCAACAGGATCATTGTCCCCAAAACCTTCTCTCAGGAGAGTTGCAAGCTTAAAAGCACAGGACAGGAATGTGAAAGTTACTCCTCTTAAGAATCAGAACAAGATCGGAGAAGTTATAGCTAAGCAACTCAACAATGATGAGACTCCGGAGAAGACCTTGTATGTCATTAAGATTGAGACTGAGAACAAACCTCTGGAATCTGATCAAAACAAAAATTGTGAAGGTCCACCACCTTCATCCTCCTCATCATCGCCCAAGTCTTTGTCCCTCCCAAACAGCATGTCCCTTTCTCCCCTTGAAGGACAAGATCAAGAGTCTGAATATACAATGACAGAAACAGAAGAGGAGTCTTTCTCAGAAGACGATGAAGTGGACAGCGAAGAAAATGCAGAGACCCTAGATGAGGATTACAAAGGGAAGCCCAGAAAGTCTGGGATGGTTTGTTCTGAAGATGTGGATAGCCATCCTTTAAAGTTTAGGAGGGGAACGGTGGTTGACATGCAATTTCAGAATAATCGACCAAGGAGGCTCAAATTTAGGCGAGGAAAAGTGTTGGGAGAGAACGAAAATGTCAAGGCTGATGCCCAAAGGCGAAGATATAAGAAGAGAGAAGGTGTTGATGGCGATGCAATTGGTACCAAACCTGGTGCAGAAAAGGTTGTTTTGAGACATCAAGATGTTCAGGGGAAGAAAGATGGGAAGGGGTTGTTTAATAATGTAATTGAAGAAACTGCAAGTAAACTGGTTGAAACCCGGAAGAGTAAGGTCAAGGCCTTAGTTGGTGCTTTTGAAACAGTGATCTCACTCCAAGAGTGCAAACCTTCTGCAAATTCGGTTTCTTGA

Protein Analysis

569

Amino Acids

62.2

Weight (kDa)

9.63

Isoelectric Point (pI)

51.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 446 - 557 5.2e-36 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1502
AccB1I GGYRCC 2 cut(s) 862, 1502
AccI GTMKAC 1 cut(s) 596
AccIII TCCGGA 1 cut(s) 1012
AciI CCGC 1 cut(s) 792
AclWI GGATC 1 cut(s) 883
AcsI RAATTY 4 cut(s) 67, 756, 1400, 1696
AcuI CTGAAG 4 cut(s) 648, 749, 759, 1335
AfaI GTAC 4 cut(s) 18, 213, 349, 1504
AfiI CCNNNNNNNGG 4 cut(s) 205, 872, 1166, 1270
AflII CTTAAG 2 cut(s) 578, 953
AflIII ACRYGT 2 cut(s) 344, 604
AgsI TTSAA 9 cut(s) 182, 463, 480, 558, 699, 1166, 1592, 1616, 1658
AjnI CCWGG 1 cut(s) 1510
AjuI GAANNNNNNNTTGG 2 cut(s) 434, 466
AloI GAACNNNNNNTCC 4 cut(s) 93, 125, 1294, 1326
AluBI AGCT 6 cut(s) 304, 395, 424, 544, 915, 986
AluI AGCT 6 cut(s) 304, 395, 424, 544, 915, 986
Alw26I GTCTC 6 cut(s) 55, 556, 1001, 1037, 1253, 1528
AlwI GGATC 1 cut(s) 883
AlwNI CAGNNNCTG 1 cut(s) 379
Ama87I CYCGRG 1 cut(s) 317
Aor13HI TCCGGA 1 cut(s) 1012
AoxI GGCC 4 cut(s) 24, 366, 706, 1638
ApeKI GCWGC 2 cut(s) 373, 746
ApoI RAATTY 4 cut(s) 67, 756, 1400, 1696
ArsI GACNNNNNNTTYG 4 cut(s) 632, 664, 1133, 1165
Asp700I GAANNNNTTC 1 cut(s) 108
Asp718I GGTACC 1 cut(s) 1502
AspS9I GGNCC 2 cut(s) 25, 1091
AsuC2I CCSGG 1 cut(s) 1621
AsuHPI GGTGA 4 cut(s) 306, 327, 466, 712
AsuII TTCGAA 1 cut(s) 617
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 1 cut(s) 1091
BaeGI GKGCMC 2 cut(s) 504, 867
BanI GGYRCC 2 cut(s) 862, 1502
BbsI GAAGAC 3 cut(s) 226, 1025, 1233
BbvI GCAGC 2 cut(s) 385, 733
BccI CCATC 6 cut(s) 362, 454, 1297, 1338, 1481, 1556
BceAI ACGGC 1 cut(s) 39
BcgI CGANNNNNNTGC 2 cut(s) 1235, 1269
BciT130I CCWGG 1 cut(s) 1512
BclI TGATCA 2 cut(s) 297, 1069
BcnI CCSGG 1 cut(s) 1621
BcoDI GTCTC 6 cut(s) 55, 556, 1001, 1037, 1253, 1528
BfaI CTAG 1 cut(s) 1265
BfmI CTRYAG 1 cut(s) 72
BfrI CTTAAG 2 cut(s) 578, 953
BglI GCCNNNNNGGC 1 cut(s) 365
BisI GCNGC 2 cut(s) 374, 747
BlpI GCTNAGC 1 cut(s) 987
BlsI GCNGC 2 cut(s) 375, 748
Bme1390I CCNGG 2 cut(s) 1512, 1621
Bme18I GGWCC 1 cut(s) 1091
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 2 cut(s) 25, 1091
BmiI GGNNCC 3 cut(s) 139, 864, 1504
BmrFI CCNGG 2 cut(s) 1512, 1621
BmsI GCATC 4 cut(s) 163, 185, 1438, 1483
BpiI GAAGAC 3 cut(s) 226, 1025, 1233
BplI GAGNNNNNCTC 2 cut(s) 1205, 1237
Bpu1102I GCTNAGC 1 cut(s) 987
Bpu14I TTCGAA 1 cut(s) 617
BpuMI CCSGG 1 cut(s) 1621
BsaBI GATNNNNATC 1 cut(s) 1329
BsaI GGTCTC 1 cut(s) 1253
BsaJI CCNNGG 1 cut(s) 1388
BsaWI WCCGGW 1 cut(s) 1012
BsaXI ACNNNNNCTCC 2 cut(s) 93, 123
Bsc4I CCNNNNNNNGG 4 cut(s) 205, 872, 1166, 1270
Bse1I ACTGG 2 cut(s) 139, 1614
Bse8I GATNNNNATC 1 cut(s) 1329
BseAI TCCGGA 1 cut(s) 1012
BseBI CCWGG 1 cut(s) 1512
BseDI CCNNGG 1 cut(s) 1388
BseGI GGATG 4 cut(s) 470, 1103, 1308, 1330
BseJI GATNNNNATC 1 cut(s) 1329
BseLI CCNNNNNNNGG 4 cut(s) 205, 872, 1166, 1270
BseMII CTCAG 6 cut(s) 136, 267, 423, 913, 1038, 1236
BseNI ACTGG 2 cut(s) 139, 1614
BseRI GAGGAG 3 cut(s) 939, 1099, 1226
BseSI GKGCMC 2 cut(s) 504, 867
BseXI GCAGC 2 cut(s) 385, 733
BsgI GTGCAG 1 cut(s) 1536
BshFI GGCC 4 cut(s) 26, 368, 708, 1640
BshNI GGYRCC 2 cut(s) 862, 1502
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 2 cut(s) 1013, 1621
BslFI GGGAC 3 cut(s) 868, 1117, 1135
BslI CCNNNNNNNGG 4 cut(s) 205, 872, 1166, 1270
BsmAI GTCTC 6 cut(s) 55, 556, 1001, 1037, 1253, 1528
BsmBI CGTCTC 1 cut(s) 55
BsmFI GGGAC 3 cut(s) 868, 1117, 1135
BsnI GGCC 4 cut(s) 26, 368, 708, 1640
Bso31I GGTCTC 1 cut(s) 1253
BsoBI CYCGRG 1 cut(s) 317
Bsp119I TTCGAA 1 cut(s) 617
Bsp1286I GDGCHC 2 cut(s) 504, 867
Bsp13I TCCGGA 1 cut(s) 1012
Bsp143I GATC 7 cut(s) 279, 297, 875, 969, 1069, 1174, 1665
Bsp1720I GCTNAGC 1 cut(s) 987
BspACI CCGC 1 cut(s) 792
BspANI GGCC 4 cut(s) 26, 368, 708, 1640
BspCNI CTCAG 6 cut(s) 135, 266, 424, 912, 1039, 1235
BspEI TCCGGA 1 cut(s) 1012
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 3 cut(s) 139, 864, 1504
BspPI GGATC 1 cut(s) 883
BspT104I TTCGAA 1 cut(s) 617
BspT107I GGYRCC 2 cut(s) 862, 1502
BspTI CTTAAG 2 cut(s) 578, 953
BspTNI GGTCTC 1 cut(s) 1253
BsrI ACTGG 2 cut(s) 139, 1614
BssECI CCNNGG 1 cut(s) 1388
BssMI GATC 7 cut(s) 279, 297, 875, 969, 1069, 1174, 1665
BssT1I CCWWGG 1 cut(s) 1388
Bst2UI CCWGG 1 cut(s) 1512
Bst4CI ACNGT 6 cut(s) 98, 511, 692, 718, 1357, 1663
Bst6I CTCTTC 6 cut(s) 177, 559, 778, 1203, 1466, 1619
BstAFI CTTAAG 2 cut(s) 578, 953
BstAPI GCANNNNNTGC 1 cut(s) 379
BstBI TTCGAA 1 cut(s) 617
BstC8I GCNNGC 2 cut(s) 366, 913
BstDEI CTNAG 8 cut(s) 122, 253, 432, 899, 987, 1047, 1222, 1642
BstENI CCTNNNNNAGG 1 cut(s) 1268
BstF5I GGATG 4 cut(s) 470, 1103, 1308, 1330
BstKTI GATC 7 cut(s) 282, 300, 878, 972, 1072, 1177, 1668
BstMAI GTCTC 6 cut(s) 55, 556, 1001, 1037, 1253, 1528
BstMBI GATC 7 cut(s) 279, 297, 875, 969, 1069, 1174, 1665
BstMWI GCNNNNNNNGC 4 cut(s) 365, 370, 379, 789
BstNI CCWGG 1 cut(s) 1512
BstNSI RCATGY 4 cut(s) 348, 608, 1150, 1369
BstSCI CCNGG 2 cut(s) 1510, 1619
BstSFI CTRYAG 1 cut(s) 72
BstSLI GKGCMC 2 cut(s) 504, 867
BstV1I GCAGC 2 cut(s) 385, 733
BstV2I GAAGAC 3 cut(s) 226, 1025, 1233
BsuRI GGCC 4 cut(s) 26, 368, 708, 1640
BtgZI GCGATG 3 cut(s) 340, 1101, 1506
BtsCI GGATG 4 cut(s) 470, 1103, 1308, 1330
BtsIMutI CAGTG 5 cut(s) 19, 103, 132, 300, 1668
Cac8I GCNNGC 2 cut(s) 366, 913
CaiI CAGNNNCTG 1 cut(s) 379
CciI TCATGA 1 cut(s) 145
Cfr13I GGNCC 2 cut(s) 25, 1091
CsiI ACCWGGT 1 cut(s) 1510
Csp6I GTAC 4 cut(s) 17, 212, 348, 1503
CviAII CATG 5 cut(s) 146, 345, 605, 1147, 1366
CviQI GTAC 4 cut(s) 17, 212, 348, 1503
DdeI CTNAG 8 cut(s) 122, 253, 432, 899, 987, 1047, 1222, 1642
DpnI GATC 7 cut(s) 281, 299, 877, 971, 1071, 1176, 1667
DpnII GATC 7 cut(s) 279, 297, 875, 969, 1069, 1174, 1665
DraI TTTAAA 2 cut(s) 849, 1338
Eam1104I CTCTTC 6 cut(s) 177, 559, 778, 1203, 1466, 1619
EarI CTCTTC 6 cut(s) 177, 559, 778, 1203, 1466, 1619
Eco130I CCWWGG 1 cut(s) 1388
Eco147I AGGCCT 1 cut(s) 1640
Eco31I GGTCTC 1 cut(s) 1253
Eco47I GGWCC 1 cut(s) 1091
Eco57I CTGAAG 4 cut(s) 648, 749, 759, 1335
Eco88I CYCGRG 1 cut(s) 317
EcoNI CCTNNNNNAGG 1 cut(s) 1268
EcoRII CCWGG 1 cut(s) 1510
EcoT14I CCWWGG 1 cut(s) 1388
EcoT22I ATGCAT 1 cut(s) 178
ErhI CCWWGG 1 cut(s) 1388
Esp3I CGTCTC 1 cut(s) 55
FaeI CATG 5 cut(s) 149, 348, 608, 1150, 1369
FaqI GGGAC 3 cut(s) 868, 1117, 1135
FatI CATG 5 cut(s) 145, 344, 604, 1146, 1365
FbaI TGATCA 2 cut(s) 297, 1069
FblI GTMKAC 1 cut(s) 596
Fnu4HI GCNGC 2 cut(s) 374, 747
FokI GGATG 4 cut(s) 457, 1090, 1315, 1317
Fsp4HI GCNGC 2 cut(s) 374, 747
FspBI CTAG 1 cut(s) 1265
GluI GCNGC 2 cut(s) 374, 747
HaeIII GGCC 4 cut(s) 26, 368, 708, 1640
HapII CCGG 2 cut(s) 1013, 1621
Hin1II CATG 5 cut(s) 149, 348, 608, 1150, 1369
HincII GTYRAC 1 cut(s) 1363
HindII GTYRAC 1 cut(s) 1363
HindIII AAGCTT 1 cut(s) 913
HinfI GANTC 8 cut(s) 222, 320, 770, 958, 1009, 1064, 1181, 1214
HpaII CCGG 2 cut(s) 1013, 1621
HphI GGTGA 4 cut(s) 306, 327, 466, 712
Hpy166II GTNNAC 7 cut(s) 17, 324, 597, 1094, 1240, 1363, 1607
Hpy8I GTNNAC 7 cut(s) 17, 324, 597, 1094, 1240, 1363, 1607
Hpy99I CGWCG 1 cut(s) 94
HpyAV CCTTC 9 cut(s) 181, 634, 903, 1082, 1110, 1160, 1472, 1561, 1698
HpyCH4III ACNGT 6 cut(s) 98, 511, 692, 718, 1357, 1663
HpyCH4IV ACGT 2 cut(s) 467, 833
HpyCH4V TGCA 9 cut(s) 176, 911, 1256, 1369, 1496, 1517, 1601, 1683, 1694
HpyF10VI GCNNNNNNNGC 4 cut(s) 365, 370, 379, 789
HpyF3I CTNAG 8 cut(s) 122, 253, 432, 899, 987, 1047, 1222, 1642
HpySE526I ACGT 2 cut(s) 467, 833
Hsp92II CATG 5 cut(s) 149, 348, 608, 1150, 1369
Kpn2I TCCGGA 1 cut(s) 1012
KpnI GGTACC 1 cut(s) 1506
Ksp22I TGATCA 2 cut(s) 297, 1069
Kzo9I GATC 7 cut(s) 279, 297, 875, 969, 1069, 1174, 1665
Lsp1109I GCAGC 2 cut(s) 385, 733
LweI GCATC 4 cut(s) 163, 185, 1438, 1483
MabI ACCWGGT 1 cut(s) 1510
MaeI CTAG 1 cut(s) 1265
MaeII ACGT 2 cut(s) 467, 833
MaeIII GTNAC 3 cut(s) 607, 718, 943
MalI GATC 7 cut(s) 281, 299, 877, 971, 1071, 1176, 1667
MboI GATC 7 cut(s) 279, 297, 875, 969, 1069, 1174, 1665
MfeI CAATTG 1 cut(s) 1497
MhlI GDGCHC 2 cut(s) 504, 867
MlyI GAGTC 6 cut(s) 216, 329, 764, 1003, 1190, 1223
MmeI TCCRAC 1 cut(s) 548
Mph1103I ATGCAT 1 cut(s) 178
MroI TCCGGA 1 cut(s) 1012
MroXI GAANNNNTTC 1 cut(s) 108
MseI TTAA 9 cut(s) 35, 579, 785, 848, 917, 954, 1035, 1337, 1578
MslI CAYNNNNRTG 1 cut(s) 1542
MspA1I CMGCKG 1 cut(s) 395
MspCI CTTAAG 2 cut(s) 578, 953
MspI CCGG 2 cut(s) 1013, 1621
MspR9I CCNGG 2 cut(s) 1512, 1621
MunI CAATTG 1 cut(s) 1497
MvaI CCWGG 1 cut(s) 1512
MwoI GCNNNNNNNGC 4 cut(s) 365, 370, 379, 789
NciI CCSGG 1 cut(s) 1621
NdeII GATC 7 cut(s) 279, 297, 875, 969, 1069, 1174, 1665
NlaIII CATG 5 cut(s) 149, 348, 608, 1150, 1369
NlaIV GGNNCC 3 cut(s) 139, 864, 1504
NmuCI GTSAC 2 cut(s) 607, 718
NsiI ATGCAT 1 cut(s) 178
NspI RCATGY 4 cut(s) 348, 608, 1150, 1369
NspV TTCGAA 1 cut(s) 617
PagI TCATGA 1 cut(s) 145
PceI AGGCCT 1 cut(s) 1640
PciI ACATGT 2 cut(s) 344, 604
PdmI GAANNNNTTC 1 cut(s) 108
PfeI GAWTC 2 cut(s) 958, 1064
PkrI GCNGC 2 cut(s) 375, 748
PleI GAGTC 6 cut(s) 216, 328, 764, 1003, 1189, 1222
PpsI GAGTC 6 cut(s) 216, 328, 764, 1003, 1189, 1222
PscI ACATGT 2 cut(s) 344, 604
Psp6I CCWGG 1 cut(s) 1510
PspGI CCWGG 1 cut(s) 1510
PspN4I GGNNCC 3 cut(s) 139, 864, 1504
PspPI GGNCC 2 cut(s) 25, 1091
PstNI CAGNNNCTG 1 cut(s) 379
PvuII CAGCTG 1 cut(s) 395
RsaI GTAC 4 cut(s) 18, 213, 349, 1504
RsaNI GTAC 4 cut(s) 17, 212, 348, 1503
RseI CAYNNNNRTG 1 cut(s) 1542
SaqAI TTAA 9 cut(s) 35, 579, 785, 848, 917, 954, 1035, 1337, 1578
SatI GCNGC 2 cut(s) 374, 747
Sau3AI GATC 7 cut(s) 279, 297, 875, 969, 1069, 1174, 1665
Sau96I GGNCC 2 cut(s) 25, 1091
SchI GAGTC 6 cut(s) 216, 329, 764, 1003, 1190, 1223
ScrFI CCNGG 2 cut(s) 1512, 1621
SduI GDGCHC 2 cut(s) 504, 867
SexAI ACCWGGT 1 cut(s) 1510
SfaNI GCATC 4 cut(s) 163, 185, 1438, 1483
SfcI CTRYAG 1 cut(s) 72
SfuI TTCGAA 1 cut(s) 617
SinI GGWCC 1 cut(s) 1091
SmiMI CAYNNNNRTG 1 cut(s) 1542
SmlI CTYRAG 2 cut(s) 578, 953
SmoI CTYRAG 2 cut(s) 578, 953
SseBI AGGCCT 1 cut(s) 1640
SsiI CCGC 1 cut(s) 792
SspMI CTAG 1 cut(s) 1265
StuI AGGCCT 1 cut(s) 1640
StyD4I CCNGG 2 cut(s) 1510, 1619
StyI CCWWGG 1 cut(s) 1388
TaaI ACNGT 6 cut(s) 98, 511, 692, 718, 1357, 1663
TaiI ACGT 2 cut(s) 470, 836
TaqI TCGA 4 cut(s) 59, 92, 617, 1384
TatI WGTACW 1 cut(s) 347
TfiI GAWTC 2 cut(s) 958, 1064
Tru1I TTAA 9 cut(s) 35, 579, 785, 848, 917, 954, 1035, 1337, 1578
Tru9I TTAA 9 cut(s) 35, 579, 785, 848, 917, 954, 1035, 1337, 1578
TscAI CASTG 5 cut(s) 19, 103, 139, 300, 1668
TseFI GTSAC 2 cut(s) 607, 718
TseI GCWGC 2 cut(s) 373, 746
Tsp45I GTSAC 2 cut(s) 607, 718
TspDTI ATGAA 2 cut(s) 1092, 1248
TspRI CASTG 5 cut(s) 19, 103, 139, 300, 1668
Vha464I CTTAAG 2 cut(s) 578, 953
VpaK11BI GGWCC 1 cut(s) 1091
XagI CCTNNNNNAGG 1 cut(s) 1268
XapI RAATTY 4 cut(s) 67, 756, 1400, 1696
XceI RCATGY 4 cut(s) 348, 608, 1150, 1369
XmiI GTMKAC 1 cut(s) 596
XmnI GAANNNNTTC 1 cut(s) 108
XspI CTAG 1 cut(s) 1265
Zsp2I ATGCAT 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.