pycom17g01290

heme protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0010327
Physical Location & Seq
Forward (+)
877110 .. 878455
1346 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g01290.1

Sequence Viewer

Length: 600 bp
ATGTCTTTAATATCTTACCGTGATCTGGTGGGTGTTGCTTATACCGAAGAGGAGACAAAGGCTATGGCAGCTGAAATTGAGGTAACTGATGGGCCTAACGATGAGGGTGAGATGTTTACTCGCCCTGGTAAACTTAGTGATCGATTCCCGGAGCCATATGCAAATGAGCAGGCAGCTAGGTTTGCTAATGGTGGAGCATATCCTCCTGATTTGAGTCTTATTACAAAGGCTCGTCACAACGGTCAGAATTATGTGTTTGCCCTTCTCACCGGTTACCGTGATCCTCCAGCTGGTGTTCAGATCCGAGAGGGCCTGCACTATAATCCTTACTTTCCTGGTGGAGCAATTGCTATGCCTAAAATGCTTAATGATGGTGCTGTTGAGTACGAAGATGGTACTCCTGCAACTGAGGCTCAGATGGGGAAAGATGTTGTATCATTCTTGACATGGGCCGCAGAACCAGAAATGGAAGAGAGGAAACTCATGGGATTCAAATGGATCTTTGTTCTCTCGCTGGCGCTACTTCAAGCTGCATACTACCGGCGCTTAAAGTGGTCAGTTCTCAAGTCCCGCAAGCTGGTTCTTGATGTTGTGAACTAA

Protein Analysis

200

Amino Acids

22.24

Weight (kDa)

5.07

Isoelectric Point (pI)

37.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_C1 PF02167 1 - 186 3e-81 Cytochrome C1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 453, 571
AclWI GGATC 3 cut(s) 275, 295, 506
AfaI GTAC 2 cut(s) 386, 397
AfiI CCNNNNNNNGG 3 cut(s) 25, 290, 577
AgeI ACCGGT 1 cut(s) 269
AgsI TTSAA 2 cut(s) 493, 527
AjnI CCWGG 2 cut(s) 124, 334
AluBI AGCT 5 cut(s) 71, 176, 290, 530, 577
AluI AGCT 5 cut(s) 71, 176, 290, 530, 577
Alw26I GTCTC 1 cut(s) 47
AlwI GGATC 3 cut(s) 275, 295, 506
AoxI GGCC 3 cut(s) 92, 310, 450
ApeKI GCWGC 3 cut(s) 68, 173, 530
AsiGI ACCGGT 1 cut(s) 269
AspLEI GCGC 2 cut(s) 520, 546
AspS9I GGNCC 3 cut(s) 92, 310, 450
AsuC2I CCSGG 1 cut(s) 149
AsuHPI GGTGA 2 cut(s) 119, 259
BbvI GCAGC 3 cut(s) 80, 185, 517
BccI CCATC 4 cut(s) 83, 365, 386, 412
BciT130I CCWGG 2 cut(s) 126, 336
BcnI CCSGG 1 cut(s) 149
BcoDI GTCTC 1 cut(s) 47
BfaI CTAG 1 cut(s) 177
BfoI RGCGCY 2 cut(s) 521, 547
BisI GCNGC 4 cut(s) 69, 174, 453, 531
BlsI GCNGC 4 cut(s) 70, 175, 454, 532
Bme1390I CCNGG 3 cut(s) 126, 149, 336
BmgT120I GGNCC 3 cut(s) 92, 310, 450
BmiI GGNNCC 1 cut(s) 153
BmrFI CCNGG 3 cut(s) 126, 149, 336
BpmI CTGGAG 1 cut(s) 270
BpuEI CTTGAG 1 cut(s) 548
BpuMI CCSGG 1 cut(s) 149
Bsa29I ATCGAT 1 cut(s) 142
BsaJI CCNNGG 1 cut(s) 124
BsaWI WCCGGW 1 cut(s) 269
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 290, 577
Bse118I RCCGGY 2 cut(s) 269, 540
BseBI CCWGG 2 cut(s) 126, 336
BseCI ATCGAT 1 cut(s) 142
BseDI CCNNGG 1 cut(s) 124
BseLI CCNNNNNNNGG 3 cut(s) 25, 290, 577
BseMII CTCAG 2 cut(s) 399, 428
BseRI GAGGAG 1 cut(s) 65
BseXI GCAGC 3 cut(s) 80, 185, 517
BsgI GTGCAG 1 cut(s) 299
BshFI GGCC 3 cut(s) 94, 312, 452
BshTI ACCGGT 1 cut(s) 269
BshVI ATCGAT 1 cut(s) 142
BsiSI CCGG 3 cut(s) 149, 270, 541
BslFI GGGAC 1 cut(s) 553
BslI CCNNNNNNNGG 3 cut(s) 25, 290, 577
BsmAI GTCTC 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 553
BsnI GGCC 3 cut(s) 94, 312, 452
Bsp143I GATC 5 cut(s) 22, 139, 280, 300, 498
BspACI CCGC 2 cut(s) 453, 571
BspANI GGCC 3 cut(s) 94, 312, 452
BspCNI CTCAG 2 cut(s) 400, 427
BspDI ATCGAT 1 cut(s) 142
BspLI GGNNCC 1 cut(s) 153
BspPI GGATC 3 cut(s) 275, 295, 506
BsrFI RCCGGY 2 cut(s) 269, 540
BssAI RCCGGY 2 cut(s) 269, 540
BssECI CCNNGG 1 cut(s) 124
BssMI GATC 5 cut(s) 22, 139, 280, 300, 498
Bst2UI CCWGG 2 cut(s) 126, 336
Bst4CI ACNGT 3 cut(s) 20, 242, 278
Bst6I CTCTTC 2 cut(s) 42, 465
BstC8I GCNNGC 4 cut(s) 171, 314, 516, 575
BstDEI CTNAG 3 cut(s) 134, 408, 414
BstEII GGTNACC 1 cut(s) 272
BstH2I RGCGCY 2 cut(s) 521, 547
BstHHI GCGC 2 cut(s) 520, 546
BstKTI GATC 5 cut(s) 25, 142, 283, 303, 501
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 5 cut(s) 22, 139, 280, 300, 498
BstMWI GCNNNNNNNGC 4 cut(s) 68, 182, 361, 410
BstNI CCWGG 2 cut(s) 126, 336
BstPI GGTNACC 1 cut(s) 272
BstSCI CCNGG 3 cut(s) 124, 147, 334
BstV1I GCAGC 3 cut(s) 80, 185, 517
BstX2I RGATCY 2 cut(s) 300, 498
BstYI RGATCY 2 cut(s) 300, 498
Bsu15I ATCGAT 1 cut(s) 142
BsuRI GGCC 3 cut(s) 94, 312, 452
BsuTUI ATCGAT 1 cut(s) 142
Cac8I GCNNGC 4 cut(s) 171, 314, 516, 575
CfoI GCGC 2 cut(s) 520, 546
Cfr10I RCCGGY 2 cut(s) 269, 540
Cfr13I GGNCC 3 cut(s) 92, 310, 450
ClaI ATCGAT 1 cut(s) 142
Csp6I GTAC 2 cut(s) 385, 396
CspAI ACCGGT 1 cut(s) 269
CviAII CATG 2 cut(s) 447, 484
CviQI GTAC 2 cut(s) 385, 396
DdeI CTNAG 3 cut(s) 134, 408, 414
DpnI GATC 5 cut(s) 24, 141, 282, 302, 500
DpnII GATC 5 cut(s) 22, 139, 280, 300, 498
Eam1104I CTCTTC 2 cut(s) 42, 465
EarI CTCTTC 2 cut(s) 42, 465
Eco91I GGTNACC 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 310
EcoO65I GGTNACC 1 cut(s) 272
EcoRII CCWGG 2 cut(s) 124, 334
FaeI CATG 2 cut(s) 450, 487
FaqI GGGAC 1 cut(s) 553
FatI CATG 2 cut(s) 446, 483
FauI CCCGC 1 cut(s) 578
FauNDI CATATG 1 cut(s) 157
Fnu4HI GCNGC 4 cut(s) 69, 174, 453, 531
Fsp4HI GCNGC 4 cut(s) 69, 174, 453, 531
FspBI CTAG 1 cut(s) 177
GlaI GCGC 2 cut(s) 519, 545
GluI GCNGC 4 cut(s) 69, 174, 453, 531
GsuI CTGGAG 1 cut(s) 270
HaeII RGCGCY 2 cut(s) 521, 547
HaeIII GGCC 3 cut(s) 94, 312, 452
HapII CCGG 3 cut(s) 149, 270, 541
HhaI GCGC 2 cut(s) 520, 546
Hin1II CATG 2 cut(s) 450, 487
Hin6I GCGC 2 cut(s) 518, 544
HinP1I GCGC 2 cut(s) 518, 544
HinfI GANTC 3 cut(s) 144, 214, 489
HpaII CCGG 3 cut(s) 149, 270, 541
HphI GGTGA 2 cut(s) 119, 259
Hpy166II GTNNAC 3 cut(s) 117, 131, 595
Hpy188I TCNGA 4 cut(s) 246, 300, 305, 417
Hpy188III TCNNGA 3 cut(s) 206, 442, 584
Hpy8I GTNNAC 3 cut(s) 117, 131, 595
HpyAV CCTTC 1 cut(s) 272
HpyCH4III ACNGT 3 cut(s) 20, 242, 278
HpyCH4V TGCA 4 cut(s) 161, 316, 404, 533
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 182, 361, 410
HpyF3I CTNAG 3 cut(s) 134, 408, 414
Hsp92II CATG 2 cut(s) 450, 487
HspAI GCGC 2 cut(s) 518, 544
Kzo9I GATC 5 cut(s) 22, 139, 280, 300, 498
LmnI GCTCC 3 cut(s) 151, 194, 341
Lsp1109I GCAGC 3 cut(s) 80, 185, 517
MaeI CTAG 1 cut(s) 177
MaeIII GTNAC 3 cut(s) 82, 233, 272
MalI GATC 5 cut(s) 24, 141, 282, 302, 500
MboI GATC 5 cut(s) 22, 139, 280, 300, 498
MboII GAAGA 3 cut(s) 59, 401, 482
MfeI CAATTG 1 cut(s) 345
MflI RGATCY 2 cut(s) 300, 498
MluCI AATT 3 cut(s) 75, 247, 345
MlyI GAGTC 1 cut(s) 223
MnlI CCTC 8 cut(s) 43, 73, 97, 213, 294, 301, 403, 468
MseI TTAA 3 cut(s) 8, 366, 548
MspA1I CMGCKG 2 cut(s) 71, 290
MspI CCGG 3 cut(s) 149, 270, 541
MspR9I CCNGG 3 cut(s) 126, 149, 336
MunI CAATTG 1 cut(s) 345
MvaI CCWGG 2 cut(s) 126, 336
MwoI GCNNNNNNNGC 4 cut(s) 68, 182, 361, 410
NciI CCSGG 1 cut(s) 149
NdeI CATATG 1 cut(s) 157
NdeII GATC 5 cut(s) 22, 139, 280, 300, 498
NlaIII CATG 2 cut(s) 450, 487
NlaIV GGNNCC 1 cut(s) 153
NmuCI GTSAC 1 cut(s) 233
PfeI GAWTC 2 cut(s) 144, 489
PfoI TCCNGGA 1 cut(s) 147
PinAI ACCGGT 1 cut(s) 269
PkrI GCNGC 4 cut(s) 70, 175, 454, 532
PleI GAGTC 1 cut(s) 222
PpsI GAGTC 1 cut(s) 222
Psp6I CCWGG 2 cut(s) 124, 334
PspEI GGTNACC 1 cut(s) 272
PspGI CCWGG 2 cut(s) 124, 334
PspN4I GGNNCC 1 cut(s) 153
PspPI GGNCC 3 cut(s) 92, 310, 450
PsuI RGATCY 2 cut(s) 300, 498
PvuII CAGCTG 2 cut(s) 71, 290
RsaI GTAC 2 cut(s) 386, 397
RsaNI GTAC 2 cut(s) 385, 396
SaqAI TTAA 3 cut(s) 8, 366, 548
SatI GCNGC 4 cut(s) 69, 174, 453, 531
Sau3AI GATC 5 cut(s) 22, 139, 280, 300, 498
Sau96I GGNCC 3 cut(s) 92, 310, 450
SchI GAGTC 1 cut(s) 223
ScrFI CCNGG 3 cut(s) 126, 149, 336
SetI ASST 7 cut(s) 73, 84, 178, 182, 292, 532, 579
SmlI CTYRAG 1 cut(s) 563
SmoI CTYRAG 1 cut(s) 563
Sse9I AATT 3 cut(s) 75, 247, 345
SsiI CCGC 2 cut(s) 453, 571
SspMI CTAG 1 cut(s) 177
StyD4I CCNGG 3 cut(s) 124, 147, 334
TaaI ACNGT 3 cut(s) 20, 242, 278
TaqI TCGA 1 cut(s) 142
TasI AATT 3 cut(s) 75, 247, 345
TauI GCSGC 1 cut(s) 455
TfiI GAWTC 2 cut(s) 144, 489
Tru1I TTAA 3 cut(s) 8, 366, 548
Tru9I TTAA 3 cut(s) 8, 366, 548
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 3 cut(s) 68, 173, 530
Tsp45I GTSAC 1 cut(s) 233
XspI CTAG 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.