MD09G1023400.v1.1

Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008999
Physical Location & Seq
Forward (+)
1404287 .. 1407130
2844 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1023400.v1.1.491

Sequence Viewer

Length: 1164 bp
ATGGCTGATGTGTATTACAGTTCGAAGAAGACTGACGATGTCTGCGACGACGTTTGTGGCCAGCAGGGATCAGAAGCAGCGTTGAGCATGGCTAGACTGCGATGCATTTTGCGGGGCTTGGATTTGAAGACTTACATTTTCCTGTTTGCGGTTGTTCCATTAGGCGTATTTGCGATATTTTTACATGGGCAGAAGATCTCGTATTTCCTAAGACCTCTTTGGGAAAAACCTCCGAAGCCCTTCATTGATATCCCACACTACTATAATCAGAATGTGTCGATGGAGACTCTTTGCAGGCTTCACGGATGGAAGATTCGTGAATCACCTAGACGAGTCTATGATGCAGTTCTTTTCAGCAATGAAGTAGACATGCTTACAATCCGATGGAATGAATTGTATCCTTTCGTGACACAGTTTGTTCTCCTCGAATCAAACTCAACGTTTACTGGCTTGCCAAAGCCATTGCTTTTTGGAAGAAACCGGGACAAGTTTAAGTTTGTTGAAAACCGGCTTACATATGTTACAACAGGAGGAAGATTCCAGAAAGGTGAAAACCCATTTTATGAGGAGGCGTATCAGAGAGTGGCACTTGACCAGCTTCTTAGAGTTGCCGGTATAGAAGATGATGACCTGCTGATCATGTCCGATGTTGATGAGATTCCTAGCGCCCACACAATTAATCTCTTGAGGTGGTGTGATGACACCCCGCCTGTTGTTCATCTTGGGCTGAGGAATTACTTGTACTCTTTTGAGTATTATGTAGATGACAAGAGCTGGAGAGCATCCGTTCACAGGTATCAGACTGGTAATACTACCTATGCACACTTCAGACAGAGCGACTACATTTTGCCAGATTCAGGTTGGCATTGCAGCTTTTGTTTCCGCCATATCAACGAGTTCATATTCAAGATGAAAGCTTACAGCCACAATGACCGGGTGAGGTTCTCTCATTATCTGAACCCTAAAAGGATCCAGGATGTAATATGTAAAGGGGCTGATCTATTCGACATGCTTCCCGAGGAGTACACATTTAAGGAGATTATCGGAAAGATGGGACCTATTCCTAGATCTCATTCAGCAGTTCATCTCCCTGCATATTTGTTGAACAATGCTGAGAAGTACAAATACCTATTGCCCGGGAACTGCAGGAGGGAAAATGGCTAA

Protein Analysis

388

Amino Acids

45.32

Weight (kDa)

7.6

Isoelectric Point (pI)

44.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_17 PF04724 40 - 385 2.1e-184 Glycosyltransferase family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 639
AccI GTMKAC 1 cut(s) 366
AciI CCGC 4 cut(s) 112, 149, 707, 883
AclI AACGTT 1 cut(s) 440
AclWI GGATC 3 cut(s) 76, 964, 977
AcoI YGGCCR 1 cut(s) 58
AcuI CTGAAG 1 cut(s) 811
AfaI GTAC 3 cut(s) 743, 1025, 1121
AfiI CCNNNNNNNGG 3 cut(s) 148, 792, 857
AgsI TTSAA 4 cut(s) 127, 503, 907, 1105
AjnI CCWGG 1 cut(s) 972
AluBI AGCT 4 cut(s) 598, 774, 873, 917
AluI AGCT 4 cut(s) 598, 774, 873, 917
Alw26I GTCTC 1 cut(s) 278
AlwI GGATC 3 cut(s) 76, 964, 977
Ama87I CYCGRG 2 cut(s) 1016, 1136
AoxI GGCC 1 cut(s) 58
ApeKI GCWGC 2 cut(s) 77, 870
AseI ATTAAT 1 cut(s) 678
Asp700I GAANNNNTTC 1 cut(s) 239
AspLEI GCGC 1 cut(s) 668
AspS9I GGNCC 1 cut(s) 1055
AsuC2I CCSGG 4 cut(s) 482, 935, 1137, 1138
AsuHPI GGTGA 3 cut(s) 315, 560, 949
AsuII TTCGAA 1 cut(s) 23
AvaI CYCGRG 2 cut(s) 1016, 1136
AvaII GGWCC 1 cut(s) 1055
BalI TGGCCA 1 cut(s) 60
BamHI GGATCC 1 cut(s) 969
BarI GAAGNNNNNNTAC 2 cut(s) 1109, 1141
BbsI GAAGAC 2 cut(s) 35, 134
BbvCI CCTCAGC 1 cut(s) 728
BbvI GCAGC 2 cut(s) 89, 882
BccI CCATC 4 cut(s) 274, 300, 378, 1045
BciT130I CCWGG 1 cut(s) 974
BciVI GTATCC 1 cut(s) 408
BclI TGATCA 1 cut(s) 636
BcnI CCSGG 4 cut(s) 482, 935, 1137, 1138
BcoDI GTCTC 1 cut(s) 278
BfaI CTAG 4 cut(s) 93, 327, 663, 1065
BfmI CTRYAG 1 cut(s) 1144
BfoI RGCGCY 1 cut(s) 669
BfuAI ACCTGC 1 cut(s) 639
BfuI GTATCC 1 cut(s) 408
BglII AGATCT 2 cut(s) 195, 1067
BisI GCNGC 2 cut(s) 78, 871
BlsI GCNGC 2 cut(s) 79, 872
Bme1390I CCNGG 5 cut(s) 482, 935, 974, 1137, 1138
Bme18I GGWCC 1 cut(s) 1055
BmeT110I CYCGRG 2 cut(s) 1016, 1136
BmgT120I GGNCC 1 cut(s) 1055
BmiI GGNNCC 2 cut(s) 971, 1056
BmrFI CCNGG 5 cut(s) 482, 935, 974, 1137, 1138
BmsI GCATC 3 cut(s) 92, 331, 791
BpiI GAAGAC 2 cut(s) 35, 134
BplI GAGNNNNNCTC 2 cut(s) 931, 963
BpmI CTGGAG 1 cut(s) 796
Bpu10I CCTNAGC 1 cut(s) 728
Bpu14I TTCGAA 1 cut(s) 23
BpuEI CTTGAG 1 cut(s) 706
BpuMI CCSGG 4 cut(s) 482, 935, 1137, 1138
BsaJI CCNNGG 2 cut(s) 1017, 1136
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 792, 857
Bse118I RCCGGY 2 cut(s) 507, 611
Bse1I ACTGG 2 cut(s) 451, 808
Bse3DI GCAATG 3 cut(s) 364, 461, 865
BseBI CCWGG 1 cut(s) 974
BseDI CCNNGG 2 cut(s) 1017, 1136
BseGI GGATG 3 cut(s) 311, 782, 982
BseLI CCNNNNNNNGG 3 cut(s) 148, 792, 857
BseMI GCAATG 3 cut(s) 364, 461, 865
BseMII CTCAG 2 cut(s) 719, 1104
BseNI ACTGG 2 cut(s) 451, 808
BseRI GAGGAG 3 cut(s) 413, 581, 1034
BseXI GCAGC 2 cut(s) 89, 882
BshFI GGCC 1 cut(s) 60
BsiHKCI CYCGRG 2 cut(s) 1016, 1136
BsiSI CCGG 5 cut(s) 481, 508, 612, 934, 1137
BslFI GGGAC 2 cut(s) 497, 1068
BslI CCNNNNNNNGG 3 cut(s) 148, 792, 857
BsmAI GTCTC 1 cut(s) 278
BsmFI GGGAC 2 cut(s) 497, 1068
BsnI GGCC 1 cut(s) 60
BsoBI CYCGRG 2 cut(s) 1016, 1136
Bsp119I TTCGAA 1 cut(s) 23
Bsp143I GATC 6 cut(s) 68, 195, 636, 969, 997, 1067
BspACI CCGC 4 cut(s) 112, 149, 707, 883
BspANI GGCC 1 cut(s) 60
BspCNI CTCAG 2 cut(s) 720, 1105
BspLI GGNNCC 2 cut(s) 971, 1056
BspMAI CTGCAG 1 cut(s) 1148
BspMI ACCTGC 1 cut(s) 639
BspPI GGATC 3 cut(s) 76, 964, 977
BspT104I TTCGAA 1 cut(s) 23
BsrDI GCAATG 3 cut(s) 364, 461, 865
BsrFI RCCGGY 2 cut(s) 507, 611
BsrI ACTGG 2 cut(s) 451, 808
BssAI RCCGGY 2 cut(s) 507, 611
BssECI CCNNGG 2 cut(s) 1017, 1136
BssMI GATC 6 cut(s) 68, 195, 636, 969, 997, 1067
Bst2UI CCWGG 1 cut(s) 974
Bst4CI ACNGT 2 cut(s) 20, 414
BstBI TTCGAA 1 cut(s) 23
BstC8I GCNNGC 3 cut(s) 62, 296, 452
BstDEI CTNAG 4 cut(s) 209, 602, 728, 1113
BstF5I GGATG 3 cut(s) 311, 782, 982
BstH2I RGCGCY 1 cut(s) 669
BstHHI GCGC 1 cut(s) 668
BstKTI GATC 6 cut(s) 71, 198, 639, 972, 1000, 1070
BstMAI GTCTC 1 cut(s) 278
BstMBI GATC 6 cut(s) 68, 195, 636, 969, 997, 1067
BstNI CCWGG 1 cut(s) 974
BstNSI RCATGY 2 cut(s) 373, 1012
BstSCI CCNGG 5 cut(s) 480, 933, 972, 1135, 1136
BstSFI CTRYAG 1 cut(s) 1144
BstV1I GCAGC 2 cut(s) 89, 882
BstV2I GAAGAC 2 cut(s) 35, 134
BstX2I RGATCY 3 cut(s) 195, 969, 1067
BstYI RGATCY 3 cut(s) 195, 969, 1067
BsuI GTATCC 1 cut(s) 408
BsuRI GGCC 1 cut(s) 60
BtgZI GCGATG 1 cut(s) 115
BtsCI GGATG 3 cut(s) 311, 782, 982
BveI ACCTGC 1 cut(s) 639
Cac8I GCNNGC 3 cut(s) 62, 296, 452
CfoI GCGC 1 cut(s) 668
Cfr10I RCCGGY 2 cut(s) 507, 611
Cfr13I GGNCC 1 cut(s) 1055
Cfr9I CCCGGG 1 cut(s) 1136
Csp6I GTAC 3 cut(s) 742, 1024, 1120
CviAII CATG 5 cut(s) 88, 185, 370, 640, 1009
CviQI GTAC 3 cut(s) 742, 1024, 1120
DdeI CTNAG 4 cut(s) 209, 602, 728, 1113
DpnI GATC 6 cut(s) 70, 197, 638, 971, 999, 1069
DpnII GATC 6 cut(s) 68, 195, 636, 969, 997, 1067
EaeI YGGCCR 1 cut(s) 58
EciI GGCGGA 1 cut(s) 872
Eco32I GATATC 1 cut(s) 250
Eco47I GGWCC 1 cut(s) 1055
Eco57I CTGAAG 1 cut(s) 811
Eco88I CYCGRG 2 cut(s) 1016, 1136
EcoO109I RGGNCCY 1 cut(s) 1055
EcoRII CCWGG 1 cut(s) 972
EcoRV GATATC 1 cut(s) 250
EcoT22I ATGCAT 1 cut(s) 107
FaeI CATG 5 cut(s) 91, 188, 373, 643, 1012
FaqI GGGAC 2 cut(s) 497, 1068
FatI CATG 5 cut(s) 87, 184, 369, 639, 1008
FauI CCCGC 2 cut(s) 105, 714
FauNDI CATATG 1 cut(s) 517
FbaI TGATCA 1 cut(s) 636
FblI GTMKAC 1 cut(s) 366
Fnu4HI GCNGC 2 cut(s) 78, 871
FokI GGATG 3 cut(s) 318, 769, 989
Fsp4HI GCNGC 2 cut(s) 78, 871
FspBI CTAG 4 cut(s) 93, 327, 663, 1065
GlaI GCGC 1 cut(s) 667
GluI GCNGC 2 cut(s) 78, 871
GsuI CTGGAG 1 cut(s) 796
HaeII RGCGCY 1 cut(s) 669
HaeIII GGCC 1 cut(s) 60
HapII CCGG 5 cut(s) 481, 508, 612, 934, 1137
HhaI GCGC 1 cut(s) 668
Hin1II CATG 5 cut(s) 91, 188, 373, 643, 1012
Hin6I GCGC 1 cut(s) 666
HinP1I GCGC 1 cut(s) 666
HindIII AAGCTT 1 cut(s) 915
HinfI GANTC 8 cut(s) 286, 313, 320, 333, 428, 537, 658, 854
HpaII CCGG 5 cut(s) 481, 508, 612, 934, 1137
HphI GGTGA 3 cut(s) 315, 560, 949
Hpy166II GTNNAC 4 cut(s) 367, 444, 790, 1026
Hpy188III TCNNGA 6 cut(s) 317, 406, 541, 685, 907, 1016
Hpy8I GTNNAC 4 cut(s) 367, 444, 790, 1026
Hpy99I CGWCG 2 cut(s) 50, 53
HpyAV CCTTC 1 cut(s) 250
HpyCH4III ACNGT 2 cut(s) 20, 414
HpyCH4IV ACGT 2 cut(s) 51, 440
HpyCH4V TGCA 7 cut(s) 105, 294, 344, 821, 870, 1094, 1146
HpyF3I CTNAG 4 cut(s) 209, 602, 728, 1113
HpySE526I ACGT 2 cut(s) 51, 440
Hsp92II CATG 5 cut(s) 91, 188, 373, 643, 1012
HspAI GCGC 1 cut(s) 666
Ksp22I TGATCA 1 cut(s) 636
Kzo9I GATC 6 cut(s) 68, 195, 636, 969, 997, 1067
Lsp1109I GCAGC 2 cut(s) 89, 882
LweI GCATC 3 cut(s) 92, 331, 791
MaeI CTAG 4 cut(s) 93, 327, 663, 1065
MaeII ACGT 2 cut(s) 51, 440
MaeIII GTNAC 2 cut(s) 406, 520
MalI GATC 6 cut(s) 70, 197, 638, 971, 999, 1069
MboI GATC 6 cut(s) 68, 195, 636, 969, 997, 1067
MboII GAAGA 8 cut(s) 37, 40, 139, 205, 322, 486, 546, 632
MflI RGATCY 3 cut(s) 195, 969, 1067
MlsI TGGCCA 1 cut(s) 60
MluCI AATT 3 cut(s) 392, 675, 733
MluNI TGGCCA 1 cut(s) 60
MlyI GAGTC 2 cut(s) 280, 342
Mox20I TGGCCA 1 cut(s) 60
Mph1103I ATGCAT 1 cut(s) 107
MroXI GAANNNNTTC 1 cut(s) 239
MscI TGGCCA 1 cut(s) 60
MseI TTAA 3 cut(s) 492, 678, 1032
Msp20I TGGCCA 1 cut(s) 60
MspI CCGG 5 cut(s) 481, 508, 612, 934, 1137
MspR9I CCNGG 5 cut(s) 482, 935, 974, 1137, 1138
MvaI CCWGG 1 cut(s) 974
NciI CCSGG 4 cut(s) 482, 935, 1137, 1138
NdeI CATATG 1 cut(s) 517
NdeII GATC 6 cut(s) 68, 195, 636, 969, 997, 1067
NlaIII CATG 5 cut(s) 91, 188, 373, 643, 1012
NlaIV GGNNCC 2 cut(s) 971, 1056
NmuCI GTSAC 1 cut(s) 406
NsiI ATGCAT 1 cut(s) 107
NspI RCATGY 2 cut(s) 373, 1012
NspV TTCGAA 1 cut(s) 23
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PdmI GAANNNNTTC 1 cut(s) 239
PfeI GAWTC 6 cut(s) 313, 320, 428, 537, 658, 854
PflFI GACNNNGTC 1 cut(s) 38
PfoI TCCNGGA 1 cut(s) 972
PkrI GCNGC 2 cut(s) 79, 872
PleI GAGTC 2 cut(s) 280, 341
PpsI GAGTC 2 cut(s) 280, 341
PpuMI RGGWCCY 1 cut(s) 1055
PshBI ATTAAT 1 cut(s) 678
Psp1406I AACGTT 1 cut(s) 440
Psp5II RGGWCCY 1 cut(s) 1055
Psp6I CCWGG 1 cut(s) 972
PspGI CCWGG 1 cut(s) 972
PspN4I GGNNCC 2 cut(s) 971, 1056
PspPI GGNCC 1 cut(s) 1055
PspPPI RGGWCCY 1 cut(s) 1055
PstI CTGCAG 1 cut(s) 1148
PsuI RGATCY 3 cut(s) 195, 969, 1067
PsyI GACNNNGTC 1 cut(s) 38
RsaI GTAC 3 cut(s) 743, 1025, 1121
RsaNI GTAC 3 cut(s) 742, 1024, 1120
SaqAI TTAA 3 cut(s) 492, 678, 1032
SatI GCNGC 2 cut(s) 78, 871
Sau3AI GATC 6 cut(s) 68, 195, 636, 969, 997, 1067
Sau96I GGNCC 1 cut(s) 1055
SchI GAGTC 2 cut(s) 280, 342
ScrFI CCNGG 5 cut(s) 482, 935, 974, 1137, 1138
SfaNI GCATC 3 cut(s) 92, 331, 791
SfcI CTRYAG 1 cut(s) 1144
SfuI TTCGAA 1 cut(s) 23
SinI GGWCC 1 cut(s) 1055
SmaI CCCGGG 1 cut(s) 1138
SmlI CTYRAG 1 cut(s) 685
SmoI CTYRAG 1 cut(s) 685
Sse9I AATT 3 cut(s) 392, 675, 733
SsiI CCGC 4 cut(s) 112, 149, 707, 883
SspMI CTAG 4 cut(s) 93, 327, 663, 1065
StyD4I CCNGG 5 cut(s) 480, 933, 972, 1135, 1136
TaaI ACNGT 2 cut(s) 20, 414
TaiI ACGT 2 cut(s) 54, 443
TaqI TCGA 4 cut(s) 23, 278, 426, 1005
TasI AATT 3 cut(s) 392, 675, 733
TatI WGTACW 3 cut(s) 741, 1023, 1119
TfiI GAWTC 6 cut(s) 313, 320, 428, 537, 658, 854
Tru1I TTAA 3 cut(s) 492, 678, 1032
Tru9I TTAA 3 cut(s) 492, 678, 1032
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 2 cut(s) 77, 870
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 7 cut(s) 232, 375, 405, 707, 889, 926, 1073
TspGWI ACGGA 2 cut(s) 318, 775
TspMI CCCGGG 1 cut(s) 1136
Tth111I GACNNNGTC 1 cut(s) 38
VpaK11BI GGWCC 1 cut(s) 1055
VspI ATTAAT 1 cut(s) 678
XceI RCATGY 2 cut(s) 373, 1012
XcmI CCANNNNNNNNNTGG 1 cut(s) 858
XmaI CCCGGG 1 cut(s) 1136
XmiI GTMKAC 1 cut(s) 366
XmnI GAANNNNTTC 1 cut(s) 239
XspI CTAG 4 cut(s) 93, 327, 663, 1065
Zsp2I ATGCAT 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.