Rw2G053110

Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0008999
Physical Location & Seq
Reverse (-)
80953398 .. 80956307
2910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G053110.1

Sequence Viewer

Length: 1161 bp
ATGTCTGATGGGTATTACAGTTCCAAGAAGACAGATGATATATGCGACGACGTTTGTGGAGAGGGATCAGAAGCAGCATTGAGCATGTCCAGACTACGATGCGTTTTGCGGGGGTTCGATTTGAAGACCTATATTTTCGTTTTTGCAGTTCTTCCATTATGCATCTTCGGTGTGTATTTACATGGGCAGAAGGTTTCGTATTTTTTACGACCTCTTTGGGAATCTCCTCCGAAGTCTTTCATTGAACTTCCACACTATTATCATGAGAATGTTTCAATGGAGACTCTCTGCAGACTTCATGGGTGGAAGACTCGTGAGTCACCAAGACGAGTTTATGATGCAGTTCTCTTCAGTAATGAGGTAGACATCCTTACAATTCGGTGGAATGAATTATATCCGTATGTCACCCAGTTTGTGCTGCTGGAATCAAACTCAACATTTACTGGCCTGCCCAAACCATTGGTTTTTGCTAAGAACCGGGACAAGTTTAAGTTTGTTGAAGACCGGCTCACTTATGTGACAACTGGAGGAAGATTTAAGAAAGGCGAGAACCCATTTTATGAGGAGGCATTTCAAAGAGTAGCACTAGACCAGCTTCTGAGAGTAGCCGGTATAGAAGATGATGATCTGCTGATAATGTCTGATGTTGACGAGATTCCTAGTGCTCACACAATTAATCTCTTGAGGTGGTGCGATGACATCCCACCTGTTGTTCATCTCAGGCTGAGGAACTACTTGTACTCTTTCGAGTATTACGTTGATGAAAAGAGCTGGAGGGCATCAGTTCACAGGTATCAAACTGGTAATACTACATATGCGCACTTCAGACAGAGTGACTACCTTTTGTCAGATGCAGGGTGGCATTGTAGCTTTTGTTTCCGCCATATCAGTGAGTTCATATTCAAGATGAAAGCTTACAGCCACTATGATCGGGTGAGGTTCTCTCATTACTTGAACCCTAAAAGGATTCAGGATGTTATATGCAAAGGGGCCGACCTATTTGACATGATTCCTGAGGAGTACACGTTTAAGGAGATTATTGGAAAGATGGGACCTATTCCTAGATCTTATTCAGCAGTTCATCTTCCTGCATATTTGCTGAACAATGCTGAGAAGTACAAGTACCTGCTGCCTGGAAACTGCAAGAGGGAAAATGGCTGA

Protein Analysis

386

Amino Acids

45.3

Weight (kDa)

6.89

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_17 PF04724 39 - 384 7.1e-187 Glycosyltransferase family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 316
Acc16I TGCGCA 1 cut(s) 819
Acc36I ACCTGC 1 cut(s) 1134
AccI GTMKAC 1 cut(s) 363
AciI CCGC 2 cut(s) 109, 880
AclWI GGATC 1 cut(s) 73
AcuI CTGAAG 2 cut(s) 334, 808
AfaI GTAC 4 cut(s) 740, 1022, 1118, 1124
AflIII ACRYGT 1 cut(s) 1023
AgsI TTSAA 7 cut(s) 124, 245, 276, 500, 575, 904, 955
AjnI CCWGG 1 cut(s) 1132
AleI CACNNNNGTG 1 cut(s) 515
AluBI AGCT 4 cut(s) 595, 771, 870, 914
AluI AGCT 4 cut(s) 595, 771, 870, 914
Alw21I GWGCWC 1 cut(s) 667
Alw26I GTCTC 1 cut(s) 275
AlwI GGATC 1 cut(s) 73
AlwNI CAGNNNCTG 1 cut(s) 598
AoxI GGCC 2 cut(s) 445, 990
ApeKI GCWGC 3 cut(s) 74, 418, 1129
AseI ATTAAT 1 cut(s) 675
Asp700I GAANNNNTTC 1 cut(s) 236
AspLEI GCGC 1 cut(s) 820
AspS9I GGNCC 2 cut(s) 990, 1052
AsuC2I CCSGG 1 cut(s) 479
AsuHPI GGTGA 3 cut(s) 312, 397, 946
AvaII GGWCC 1 cut(s) 1052
AxyI CCTNAGG 1 cut(s) 1014
BarI GAAGNNNNNNTAC 2 cut(s) 1106, 1138
BauI CACGAG 1 cut(s) 312
BbsI GAAGAC 4 cut(s) 35, 131, 314, 507
Bbv12I GWGCWC 1 cut(s) 667
BbvCI CCTCAGC 1 cut(s) 725
BbvI GCAGC 3 cut(s) 86, 405, 1116
BccI CCATC 2 cut(s) 2, 1042
BciT130I CCWGG 1 cut(s) 1134
BcnI CCSGG 1 cut(s) 479
BcoDI GTCTC 1 cut(s) 275
BfaI CTAG 3 cut(s) 587, 660, 1062
BfmI CTRYAG 1 cut(s) 289
BfuAI ACCTGC 1 cut(s) 1134
BglII AGATCT 1 cut(s) 1064
BisI GCNGC 3 cut(s) 75, 419, 1130
BlsI GCNGC 3 cut(s) 76, 420, 1131
Bme1390I CCNGG 2 cut(s) 479, 1134
Bme18I GGWCC 1 cut(s) 1052
BmgT120I GGNCC 2 cut(s) 990, 1052
BmiI GGNNCC 2 cut(s) 991, 1053
BmrFI CCNGG 2 cut(s) 479, 1134
BmrI ACTGGG 1 cut(s) 403
BmsI GCATC 5 cut(s) 89, 171, 328, 788, 841
BmuI ACTGGG 1 cut(s) 403
BpiI GAAGAC 4 cut(s) 35, 131, 314, 507
BplI GAGNNNNNCTC 2 cut(s) 928, 960
BpmI CTGGAG 2 cut(s) 546, 793
Bpu10I CCTNAGC 1 cut(s) 725
BpuEI CTTGAG 1 cut(s) 703
BpuMI CCSGG 1 cut(s) 479
BsaBI GATNNNNATC 1 cut(s) 624
BsaXI ACNNNNNCTCC 2 cut(s) 1010, 1040
Bse118I RCCGGY 2 cut(s) 504, 608
Bse1I ACTGG 4 cut(s) 409, 448, 529, 805
Bse21I CCTNAGG 1 cut(s) 1014
Bse8I GATNNNNATC 1 cut(s) 624
BseBI CCWGG 1 cut(s) 1134
BseGI GGATG 3 cut(s) 366, 699, 979
BseJI GATNNNNATC 1 cut(s) 624
BseMII CTCAG 5 cut(s) 590, 716, 733, 1005, 1101
BseNI ACTGG 4 cut(s) 409, 448, 529, 805
BseRI GAGGAG 3 cut(s) 216, 578, 1031
BseXI GCAGC 3 cut(s) 86, 405, 1116
BshFI GGCC 2 cut(s) 447, 992
BsiHKAI GWGCWC 1 cut(s) 667
BsiSI CCGG 3 cut(s) 478, 505, 609
BslFI GGGAC 2 cut(s) 494, 1065
BsmAI GTCTC 1 cut(s) 275
BsmFI GGGAC 2 cut(s) 494, 1065
BsnI GGCC 2 cut(s) 447, 992
Bsp1286I GDGCHC 1 cut(s) 667
Bsp143I GATC 4 cut(s) 65, 625, 928, 1064
BspACI CCGC 2 cut(s) 109, 880
BspANI GGCC 2 cut(s) 447, 992
BspCNI CTCAG 5 cut(s) 591, 717, 732, 1006, 1102
BspHI TCATGA 1 cut(s) 262
BspLI GGNNCC 2 cut(s) 991, 1053
BspMAI CTGCAG 1 cut(s) 293
BspMI ACCTGC 1 cut(s) 1134
BspPI GGATC 1 cut(s) 73
BsrFI RCCGGY 2 cut(s) 504, 608
BsrI ACTGG 4 cut(s) 409, 448, 529, 805
BssAI RCCGGY 2 cut(s) 504, 608
BssMI GATC 4 cut(s) 65, 625, 928, 1064
BssSI CACGAG 1 cut(s) 312
Bst2BI CACGAG 1 cut(s) 312
Bst2UI CCWGG 1 cut(s) 1134
Bst4CI ACNGT 1 cut(s) 20
Bst6I CTCTTC 1 cut(s) 353
BstC8I GCNNGC 1 cut(s) 449
BstDEI CTNAG 6 cut(s) 471, 599, 719, 725, 1014, 1110
BstF5I GGATG 3 cut(s) 366, 699, 979
BstHHI GCGC 1 cut(s) 820
BstKTI GATC 4 cut(s) 68, 628, 931, 1067
BstMAI GTCTC 1 cut(s) 275
BstMBI GATC 4 cut(s) 65, 625, 928, 1064
BstNI CCWGG 1 cut(s) 1134
BstNSI RCATGY 1 cut(s) 88
BstSCI CCNGG 2 cut(s) 477, 1132
BstSFI CTRYAG 1 cut(s) 289
BstV1I GCAGC 3 cut(s) 86, 405, 1116
BstV2I GAAGAC 4 cut(s) 35, 131, 314, 507
BstX2I RGATCY 1 cut(s) 1064
BstXI CCANNNNNNTGG 1 cut(s) 460
BstYI RGATCY 1 cut(s) 1064
Bsu36I CCTNAGG 1 cut(s) 1014
BsuRI GGCC 2 cut(s) 447, 992
BtgZI GCGATG 1 cut(s) 708
BtsCI GGATG 3 cut(s) 366, 699, 979
BtsIMutI CAGTG 1 cut(s) 895
BveI ACCTGC 1 cut(s) 1134
Cac8I GCNNGC 1 cut(s) 449
CaiI CAGNNNCTG 1 cut(s) 598
CciI TCATGA 1 cut(s) 262
CfoI GCGC 1 cut(s) 820
Cfr10I RCCGGY 2 cut(s) 504, 608
Cfr13I GGNCC 2 cut(s) 990, 1052
Csp6I GTAC 4 cut(s) 739, 1021, 1117, 1123
CviAII CATG 5 cut(s) 85, 182, 263, 299, 1006
CviQI GTAC 4 cut(s) 739, 1021, 1117, 1123
DdeI CTNAG 6 cut(s) 471, 599, 719, 725, 1014, 1110
DpnI GATC 4 cut(s) 67, 627, 930, 1066
DpnII GATC 4 cut(s) 65, 625, 928, 1064
DrdI GACNNNNNNGTC 1 cut(s) 316
DseDI GACNNNNNNGTC 1 cut(s) 316
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
EciI GGCGGA 1 cut(s) 869
Eco47I GGWCC 1 cut(s) 1052
Eco57I CTGAAG 2 cut(s) 334, 808
Eco81I CCTNAGG 1 cut(s) 1014
EcoO109I RGGNCCY 1 cut(s) 1052
EcoRII CCWGG 1 cut(s) 1132
EcoT22I ATGCAT 1 cut(s) 164
FaeI CATG 5 cut(s) 88, 185, 266, 302, 1009
FaqI GGGAC 2 cut(s) 494, 1065
FatI CATG 5 cut(s) 84, 181, 262, 298, 1005
FauI CCCGC 1 cut(s) 102
FauNDI CATATG 1 cut(s) 814
FblI GTMKAC 1 cut(s) 363
Fnu4HI GCNGC 3 cut(s) 75, 419, 1130
FokI GGATG 3 cut(s) 353, 686, 986
Fsp4HI GCNGC 3 cut(s) 75, 419, 1130
FspAI RTGCGCAY 1 cut(s) 819
FspBI CTAG 3 cut(s) 587, 660, 1062
FspI TGCGCA 1 cut(s) 819
GlaI GCGC 1 cut(s) 819
GluI GCNGC 3 cut(s) 75, 419, 1130
GsuI CTGGAG 2 cut(s) 546, 793
HaeIII GGCC 2 cut(s) 447, 992
HapII CCGG 3 cut(s) 478, 505, 609
HhaI GCGC 1 cut(s) 820
Hin1II CATG 5 cut(s) 88, 185, 266, 302, 1009
Hin6I GCGC 1 cut(s) 818
HinP1I GCGC 1 cut(s) 818
HincII GTYRAC 1 cut(s) 649
HindII GTYRAC 1 cut(s) 649
HindIII AAGCTT 1 cut(s) 912
HinfI GANTC 8 cut(s) 221, 283, 310, 317, 425, 655, 967, 1009
HpaII CCGG 3 cut(s) 478, 505, 609
HphI GGTGA 3 cut(s) 312, 397, 946
Hpy166II GTNNAC 4 cut(s) 364, 649, 787, 1023
Hpy188I TCNGA 7 cut(s) 7, 70, 231, 600, 643, 827, 850
Hpy188III TCNNGA 7 cut(s) 90, 263, 314, 682, 904, 971, 1013
Hpy8I GTNNAC 4 cut(s) 364, 649, 787, 1023
Hpy99I CGWCG 2 cut(s) 50, 53
HpyAV CCTTC 1 cut(s) 184
HpyCH4III ACNGT 1 cut(s) 20
HpyCH4IV ACGT 3 cut(s) 51, 756, 1025
HpyCH4V TGCA 8 cut(s) 146, 162, 291, 341, 854, 984, 1091, 1143
HpyF3I CTNAG 6 cut(s) 471, 599, 719, 725, 1014, 1110
HpySE526I ACGT 3 cut(s) 51, 756, 1025
Hsp92II CATG 5 cut(s) 88, 185, 266, 302, 1009
HspAI GCGC 1 cut(s) 818
Kzo9I GATC 4 cut(s) 65, 625, 928, 1064
Lsp1109I GCAGC 3 cut(s) 86, 405, 1116
LweI GCATC 5 cut(s) 89, 171, 328, 788, 841
MaeI CTAG 3 cut(s) 587, 660, 1062
MaeII ACGT 3 cut(s) 51, 756, 1025
MaeIII GTNAC 4 cut(s) 318, 403, 517, 833
MalI GATC 4 cut(s) 67, 627, 930, 1066
MboI GATC 4 cut(s) 65, 625, 928, 1064
MflI RGATCY 1 cut(s) 1064
MhlI GDGCHC 1 cut(s) 667
MluCI AATT 3 cut(s) 375, 389, 672
MlyI GAGTC 3 cut(s) 277, 304, 326
Mph1103I ATGCAT 1 cut(s) 164
MroXI GAANNNNTTC 1 cut(s) 236
MseI TTAA 4 cut(s) 489, 537, 675, 1029
MslI CAYNNNNRTG 3 cut(s) 267, 515, 888
MspI CCGG 3 cut(s) 478, 505, 609
MspR9I CCNGG 2 cut(s) 479, 1134
MvaI CCWGG 1 cut(s) 1134
NciI CCSGG 1 cut(s) 479
NdeI CATATG 1 cut(s) 814
NdeII GATC 4 cut(s) 65, 625, 928, 1064
NlaIII CATG 5 cut(s) 88, 185, 266, 302, 1009
NlaIV GGNNCC 2 cut(s) 991, 1053
NmuCI GTSAC 4 cut(s) 318, 403, 517, 833
NsbI TGCGCA 1 cut(s) 819
NsiI ATGCAT 1 cut(s) 164
NspI RCATGY 1 cut(s) 88
OliI CACNNNNGTG 1 cut(s) 515
PagI TCATGA 1 cut(s) 262
PcsI WCGNNNNNNNCGW 1 cut(s) 753
PdmI GAANNNNTTC 1 cut(s) 236
PfeI GAWTC 5 cut(s) 221, 425, 655, 967, 1009
PkrI GCNGC 3 cut(s) 76, 420, 1131
PleI GAGTC 3 cut(s) 277, 304, 325
PpsI GAGTC 3 cut(s) 277, 304, 325
PpuMI RGGWCCY 1 cut(s) 1052
PshBI ATTAAT 1 cut(s) 675
Psp5II RGGWCCY 1 cut(s) 1052
Psp6I CCWGG 1 cut(s) 1132
PspGI CCWGG 1 cut(s) 1132
PspN4I GGNNCC 2 cut(s) 991, 1053
PspPI GGNCC 2 cut(s) 990, 1052
PspPPI RGGWCCY 1 cut(s) 1052
PsrI GAACNNNNNNTAC 2 cut(s) 722, 754
PstI CTGCAG 1 cut(s) 293
PstNI CAGNNNCTG 1 cut(s) 598
PsuI RGATCY 1 cut(s) 1064
RsaI GTAC 4 cut(s) 740, 1022, 1118, 1124
RsaNI GTAC 4 cut(s) 739, 1021, 1117, 1123
RseI CAYNNNNRTG 3 cut(s) 267, 515, 888
SaqAI TTAA 4 cut(s) 489, 537, 675, 1029
SatI GCNGC 3 cut(s) 75, 419, 1130
Sau3AI GATC 4 cut(s) 65, 625, 928, 1064
Sau96I GGNCC 2 cut(s) 990, 1052
SchI GAGTC 3 cut(s) 277, 304, 326
ScrFI CCNGG 2 cut(s) 479, 1134
SduI GDGCHC 1 cut(s) 667
SfaNI GCATC 5 cut(s) 89, 171, 328, 788, 841
SfcI CTRYAG 1 cut(s) 289
SinI GGWCC 1 cut(s) 1052
SmiMI CAYNNNNRTG 3 cut(s) 267, 515, 888
SmlI CTYRAG 1 cut(s) 682
SmoI CTYRAG 1 cut(s) 682
Sse9I AATT 3 cut(s) 375, 389, 672
SsiI CCGC 2 cut(s) 109, 880
SspMI CTAG 3 cut(s) 587, 660, 1062
StyD4I CCNGG 2 cut(s) 477, 1132
TaaI ACNGT 1 cut(s) 20
TaiI ACGT 3 cut(s) 54, 759, 1028
TaqI TCGA 2 cut(s) 117, 747
TasI AATT 3 cut(s) 375, 389, 672
TatI WGTACW 3 cut(s) 738, 1020, 1116
TfiI GAWTC 5 cut(s) 221, 425, 655, 967, 1009
Tru1I TTAA 4 cut(s) 489, 537, 675, 1029
Tru9I TTAA 4 cut(s) 489, 537, 675, 1029
TscAI CASTG 1 cut(s) 895
TseFI GTSAC 4 cut(s) 318, 403, 517, 833
TseI GCWGC 3 cut(s) 74, 418, 1129
Tsp45I GTSAC 4 cut(s) 318, 403, 517, 833
TspDTI ATGAA 8 cut(s) 229, 287, 402, 704, 777, 886, 923, 1070
TspGWI ACGGA 1 cut(s) 387
TspRI CASTG 1 cut(s) 895
VpaK11BI GGWCC 1 cut(s) 1052
VspI ATTAAT 1 cut(s) 675
XceI RCATGY 1 cut(s) 88
XmiI GTMKAC 1 cut(s) 363
XmnI GAANNNNTTC 1 cut(s) 236
XspI CTAG 3 cut(s) 587, 660, 1062
Zsp2I ATGCAT 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.