Rh2CG622500

Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0008999
Physical Location & Seq
Reverse (-)
79657079 .. 79660300
3222 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG622500.1

Sequence Viewer

Length: 1164 bp
ATGTCTGATGGGTATTATAGTTCCAAGAAGACAGATGATATATGCGACGACGTTTGTGGAGAGCAGGGATCAGAAGCAGCATTGAGCATGTCCAGACTACGATGCGTTTTGCGGGGGTTCGATTTGAAGACCTATATTTTCGTTTTTGCAGTTCTTCCATTATGCATCTTCGGTGTGTATTTACATGGGCAGAAGGTTTCGTATTTTTTACGACCTCTTTGGGAATCTCCTCCGAAGTCTTTCATTGAACTTCCACACTATTATCATGAGAATGTTTCAATGGAGACTCTCTGCAGACTTCATGGGTGGAAGACTCGTGAGTCACCAAGACGAGTTTATGATGCAGTTCTCTTCAGTAATGAGGTAGACATCCTTACAATTCGGTGGAATGAATTATATCCGTATGTCACCCAGTTTGTGCTGCTGGAATCAAACTCAACATTTACTGGCCTGCCCAAACCATTGGTTTTTGCTAAGAACCGGGACAAGTTTAAGTTTGTTGAAGACCGGCTCACTTATGTGACAACTGGAGGAAGATTTAAGAAAGGCGAGAACCCATTTTATGAGGAGGCATTTCAAAGAGTAGCACTAGACCAGCTTCTGAGAGTAGCCGGTATAGAAGATGATGATCTGCTGATAATGTCTGATGTTGACGAGATTCCTAGTGCTCACACAATTAATCTCTTGAGGTGGTGCGATGACATCCCACCTGTTGTTCATCTCAGGCTGAGGAACTACTTGTACTCTTTCGAGTATTATGTTGATAAAAAGAGCTGGAGGGCATCAGTTCACAGGTATCAAACTGGTAATACTACATATGCGCACTTCAGACAGAGTGACTACCTTTTGTCAGATGCAGGGTGGCATTGTAGCTTTTGTTTCCGCCATATCAGTGAGTTCATATTCAAGATGAAAGCTTACAGCCACTATGATCGGGTGAGGTTCTCTCATTACTTGAACCCTAAAAGGATTCAGGATGTTATATGCAAAGGGGCCGACCTATTTGACATGATTCCTGAGGAGTACACGTTTAAGGAGATTATTGGAAAGATGGGACCTATTCCTAGATCTTATTCAGCAGTTCATCTTCCTGCATATTTGCTGAACAATGCTGAGAAGTACAAGTACCTGCTGCCTGGAAACTGCAAGAGGGAAAATGGCTGA

Protein Analysis

387

Amino Acids

45.43

Weight (kDa)

7.58

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_17 PF04724 40 - 385 6.9e-187 Glycosyltransferase family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 319
Acc16I TGCGCA 1 cut(s) 822
Acc36I ACCTGC 1 cut(s) 1137
AccI GTMKAC 1 cut(s) 366
AciI CCGC 2 cut(s) 112, 883
AclWI GGATC 1 cut(s) 76
AcuI CTGAAG 2 cut(s) 337, 811
AfaI GTAC 4 cut(s) 743, 1025, 1121, 1127
AflIII ACRYGT 1 cut(s) 1026
AgsI TTSAA 7 cut(s) 127, 248, 279, 503, 578, 907, 958
AjnI CCWGG 1 cut(s) 1135
AleI CACNNNNGTG 1 cut(s) 518
AluBI AGCT 4 cut(s) 598, 774, 873, 917
AluI AGCT 4 cut(s) 598, 774, 873, 917
Alw21I GWGCWC 1 cut(s) 670
Alw26I GTCTC 1 cut(s) 278
AlwI GGATC 1 cut(s) 76
AlwNI CAGNNNCTG 1 cut(s) 601
AoxI GGCC 2 cut(s) 448, 993
ApeKI GCWGC 3 cut(s) 77, 421, 1132
AseI ATTAAT 1 cut(s) 678
Asp700I GAANNNNTTC 1 cut(s) 239
AspLEI GCGC 1 cut(s) 823
AspS9I GGNCC 2 cut(s) 993, 1055
AsuC2I CCSGG 1 cut(s) 482
AsuHPI GGTGA 3 cut(s) 315, 400, 949
AvaII GGWCC 1 cut(s) 1055
AxyI CCTNAGG 1 cut(s) 1017
BarI GAAGNNNNNNTAC 2 cut(s) 1109, 1141
BauI CACGAG 1 cut(s) 315
BbsI GAAGAC 4 cut(s) 35, 134, 317, 510
Bbv12I GWGCWC 1 cut(s) 670
BbvCI CCTCAGC 1 cut(s) 728
BbvI GCAGC 3 cut(s) 89, 408, 1119
BccI CCATC 2 cut(s) 2, 1045
BciT130I CCWGG 1 cut(s) 1137
BcnI CCSGG 1 cut(s) 482
BcoDI GTCTC 1 cut(s) 278
BfaI CTAG 3 cut(s) 590, 663, 1065
BfmI CTRYAG 1 cut(s) 292
BfuAI ACCTGC 1 cut(s) 1137
BglII AGATCT 1 cut(s) 1067
BisI GCNGC 3 cut(s) 78, 422, 1133
BlsI GCNGC 3 cut(s) 79, 423, 1134
Bme1390I CCNGG 2 cut(s) 482, 1137
Bme18I GGWCC 1 cut(s) 1055
BmgT120I GGNCC 2 cut(s) 993, 1055
BmiI GGNNCC 2 cut(s) 994, 1056
BmrFI CCNGG 2 cut(s) 482, 1137
BmrI ACTGGG 1 cut(s) 406
BmsI GCATC 5 cut(s) 92, 174, 331, 791, 844
BmuI ACTGGG 1 cut(s) 406
BpiI GAAGAC 4 cut(s) 35, 134, 317, 510
BplI GAGNNNNNCTC 2 cut(s) 931, 963
BpmI CTGGAG 2 cut(s) 549, 796
Bpu10I CCTNAGC 1 cut(s) 728
BpuEI CTTGAG 1 cut(s) 706
BpuMI CCSGG 1 cut(s) 482
BsaBI GATNNNNATC 1 cut(s) 627
BsaXI ACNNNNNCTCC 2 cut(s) 1013, 1043
Bse118I RCCGGY 2 cut(s) 507, 611
Bse1I ACTGG 4 cut(s) 412, 451, 532, 808
Bse21I CCTNAGG 1 cut(s) 1017
Bse8I GATNNNNATC 1 cut(s) 627
BseBI CCWGG 1 cut(s) 1137
BseGI GGATG 3 cut(s) 369, 702, 982
BseJI GATNNNNATC 1 cut(s) 627
BseMII CTCAG 5 cut(s) 593, 719, 736, 1008, 1104
BseNI ACTGG 4 cut(s) 412, 451, 532, 808
BseRI GAGGAG 3 cut(s) 219, 581, 1034
BseXI GCAGC 3 cut(s) 89, 408, 1119
BshFI GGCC 2 cut(s) 450, 995
BsiHKAI GWGCWC 1 cut(s) 670
BsiSI CCGG 3 cut(s) 481, 508, 612
BslFI GGGAC 2 cut(s) 497, 1068
BsmAI GTCTC 1 cut(s) 278
BsmFI GGGAC 2 cut(s) 497, 1068
BsnI GGCC 2 cut(s) 450, 995
Bsp1286I GDGCHC 1 cut(s) 670
Bsp143I GATC 4 cut(s) 68, 628, 931, 1067
BspACI CCGC 2 cut(s) 112, 883
BspANI GGCC 2 cut(s) 450, 995
BspCNI CTCAG 5 cut(s) 594, 720, 735, 1009, 1105
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 2 cut(s) 994, 1056
BspMAI CTGCAG 1 cut(s) 296
BspMI ACCTGC 1 cut(s) 1137
BspPI GGATC 1 cut(s) 76
BsrFI RCCGGY 2 cut(s) 507, 611
BsrI ACTGG 4 cut(s) 412, 451, 532, 808
BssAI RCCGGY 2 cut(s) 507, 611
BssMI GATC 4 cut(s) 68, 628, 931, 1067
BssSI CACGAG 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 315
Bst2UI CCWGG 1 cut(s) 1137
Bst6I CTCTTC 1 cut(s) 356
BstC8I GCNNGC 1 cut(s) 452
BstDEI CTNAG 6 cut(s) 474, 602, 722, 728, 1017, 1113
BstF5I GGATG 3 cut(s) 369, 702, 982
BstHHI GCGC 1 cut(s) 823
BstKTI GATC 4 cut(s) 71, 631, 934, 1070
BstMAI GTCTC 1 cut(s) 278
BstMBI GATC 4 cut(s) 68, 628, 931, 1067
BstNI CCWGG 1 cut(s) 1137
BstNSI RCATGY 1 cut(s) 91
BstSCI CCNGG 2 cut(s) 480, 1135
BstSFI CTRYAG 1 cut(s) 292
BstV1I GCAGC 3 cut(s) 89, 408, 1119
BstV2I GAAGAC 4 cut(s) 35, 134, 317, 510
BstX2I RGATCY 1 cut(s) 1067
BstXI CCANNNNNNTGG 1 cut(s) 463
BstYI RGATCY 1 cut(s) 1067
Bsu36I CCTNAGG 1 cut(s) 1017
BsuRI GGCC 2 cut(s) 450, 995
BtgZI GCGATG 1 cut(s) 711
BtsCI GGATG 3 cut(s) 369, 702, 982
BtsIMutI CAGTG 1 cut(s) 898
BveI ACCTGC 1 cut(s) 1137
Cac8I GCNNGC 1 cut(s) 452
CaiI CAGNNNCTG 1 cut(s) 601
CciI TCATGA 1 cut(s) 265
CfoI GCGC 1 cut(s) 823
Cfr10I RCCGGY 2 cut(s) 507, 611
Cfr13I GGNCC 2 cut(s) 993, 1055
Csp6I GTAC 4 cut(s) 742, 1024, 1120, 1126
CviAII CATG 5 cut(s) 88, 185, 266, 302, 1009
CviQI GTAC 4 cut(s) 742, 1024, 1120, 1126
DdeI CTNAG 6 cut(s) 474, 602, 722, 728, 1017, 1113
DpnI GATC 4 cut(s) 70, 630, 933, 1069
DpnII GATC 4 cut(s) 68, 628, 931, 1067
DrdI GACNNNNNNGTC 1 cut(s) 319
DseDI GACNNNNNNGTC 1 cut(s) 319
Eam1104I CTCTTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 356
EciI GGCGGA 1 cut(s) 872
Eco47I GGWCC 1 cut(s) 1055
Eco57I CTGAAG 2 cut(s) 337, 811
Eco81I CCTNAGG 1 cut(s) 1017
EcoO109I RGGNCCY 1 cut(s) 1055
EcoRII CCWGG 1 cut(s) 1135
EcoT22I ATGCAT 1 cut(s) 167
FaeI CATG 5 cut(s) 91, 188, 269, 305, 1012
FaqI GGGAC 2 cut(s) 497, 1068
FatI CATG 5 cut(s) 87, 184, 265, 301, 1008
FauI CCCGC 1 cut(s) 105
FauNDI CATATG 1 cut(s) 817
FblI GTMKAC 1 cut(s) 366
Fnu4HI GCNGC 3 cut(s) 78, 422, 1133
FokI GGATG 3 cut(s) 356, 689, 989
Fsp4HI GCNGC 3 cut(s) 78, 422, 1133
FspAI RTGCGCAY 1 cut(s) 822
FspBI CTAG 3 cut(s) 590, 663, 1065
FspI TGCGCA 1 cut(s) 822
GlaI GCGC 1 cut(s) 822
GluI GCNGC 3 cut(s) 78, 422, 1133
GsuI CTGGAG 2 cut(s) 549, 796
HaeIII GGCC 2 cut(s) 450, 995
HapII CCGG 3 cut(s) 481, 508, 612
HhaI GCGC 1 cut(s) 823
Hin1II CATG 5 cut(s) 91, 188, 269, 305, 1012
Hin6I GCGC 1 cut(s) 821
HinP1I GCGC 1 cut(s) 821
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HindIII AAGCTT 1 cut(s) 915
HinfI GANTC 8 cut(s) 224, 286, 313, 320, 428, 658, 970, 1012
HpaII CCGG 3 cut(s) 481, 508, 612
HphI GGTGA 3 cut(s) 315, 400, 949
Hpy166II GTNNAC 4 cut(s) 367, 652, 790, 1026
Hpy188I TCNGA 7 cut(s) 7, 73, 234, 603, 646, 830, 853
Hpy188III TCNNGA 7 cut(s) 93, 266, 317, 685, 907, 974, 1016
Hpy8I GTNNAC 4 cut(s) 367, 652, 790, 1026
Hpy99I CGWCG 2 cut(s) 50, 53
HpyAV CCTTC 1 cut(s) 187
HpyCH4IV ACGT 2 cut(s) 51, 1028
HpyCH4V TGCA 8 cut(s) 149, 165, 294, 344, 857, 987, 1094, 1146
HpyF3I CTNAG 6 cut(s) 474, 602, 722, 728, 1017, 1113
HpySE526I ACGT 2 cut(s) 51, 1028
Hsp92II CATG 5 cut(s) 91, 188, 269, 305, 1012
HspAI GCGC 1 cut(s) 821
Kzo9I GATC 4 cut(s) 68, 628, 931, 1067
Lsp1109I GCAGC 3 cut(s) 89, 408, 1119
LweI GCATC 5 cut(s) 92, 174, 331, 791, 844
MaeI CTAG 3 cut(s) 590, 663, 1065
MaeII ACGT 2 cut(s) 51, 1028
MaeIII GTNAC 4 cut(s) 321, 406, 520, 836
MalI GATC 4 cut(s) 70, 630, 933, 1069
MboI GATC 4 cut(s) 68, 628, 931, 1067
MflI RGATCY 1 cut(s) 1067
MhlI GDGCHC 1 cut(s) 670
MluCI AATT 3 cut(s) 378, 392, 675
MlyI GAGTC 3 cut(s) 280, 307, 329
Mph1103I ATGCAT 1 cut(s) 167
MroXI GAANNNNTTC 1 cut(s) 239
MseI TTAA 4 cut(s) 492, 540, 678, 1032
MslI CAYNNNNRTG 3 cut(s) 270, 518, 891
MspI CCGG 3 cut(s) 481, 508, 612
MspR9I CCNGG 2 cut(s) 482, 1137
MvaI CCWGG 1 cut(s) 1137
NciI CCSGG 1 cut(s) 482
NdeI CATATG 1 cut(s) 817
NdeII GATC 4 cut(s) 68, 628, 931, 1067
NlaIII CATG 5 cut(s) 91, 188, 269, 305, 1012
NlaIV GGNNCC 2 cut(s) 994, 1056
NmuCI GTSAC 4 cut(s) 321, 406, 520, 836
NsbI TGCGCA 1 cut(s) 822
NsiI ATGCAT 1 cut(s) 167
NspI RCATGY 1 cut(s) 91
OliI CACNNNNGTG 1 cut(s) 518
PagI TCATGA 1 cut(s) 265
PdmI GAANNNNTTC 1 cut(s) 239
PfeI GAWTC 5 cut(s) 224, 428, 658, 970, 1012
PkrI GCNGC 3 cut(s) 79, 423, 1134
PleI GAGTC 3 cut(s) 280, 307, 328
PpsI GAGTC 3 cut(s) 280, 307, 328
PpuMI RGGWCCY 1 cut(s) 1055
PshBI ATTAAT 1 cut(s) 678
Psp5II RGGWCCY 1 cut(s) 1055
Psp6I CCWGG 1 cut(s) 1135
PspGI CCWGG 1 cut(s) 1135
PspN4I GGNNCC 2 cut(s) 994, 1056
PspPI GGNCC 2 cut(s) 993, 1055
PspPPI RGGWCCY 1 cut(s) 1055
PsrI GAACNNNNNNTAC 2 cut(s) 725, 757
PstI CTGCAG 1 cut(s) 296
PstNI CAGNNNCTG 1 cut(s) 601
PsuI RGATCY 1 cut(s) 1067
RsaI GTAC 4 cut(s) 743, 1025, 1121, 1127
RsaNI GTAC 4 cut(s) 742, 1024, 1120, 1126
RseI CAYNNNNRTG 3 cut(s) 270, 518, 891
SaqAI TTAA 4 cut(s) 492, 540, 678, 1032
SatI GCNGC 3 cut(s) 78, 422, 1133
Sau3AI GATC 4 cut(s) 68, 628, 931, 1067
Sau96I GGNCC 2 cut(s) 993, 1055
SchI GAGTC 3 cut(s) 280, 307, 329
ScrFI CCNGG 2 cut(s) 482, 1137
SduI GDGCHC 1 cut(s) 670
SfaNI GCATC 5 cut(s) 92, 174, 331, 791, 844
SfcI CTRYAG 1 cut(s) 292
SinI GGWCC 1 cut(s) 1055
SmiMI CAYNNNNRTG 3 cut(s) 270, 518, 891
SmlI CTYRAG 1 cut(s) 685
SmoI CTYRAG 1 cut(s) 685
Sse9I AATT 3 cut(s) 378, 392, 675
SsiI CCGC 2 cut(s) 112, 883
SspMI CTAG 3 cut(s) 590, 663, 1065
StyD4I CCNGG 2 cut(s) 480, 1135
TaiI ACGT 2 cut(s) 54, 1031
TaqI TCGA 2 cut(s) 120, 750
TasI AATT 3 cut(s) 378, 392, 675
TatI WGTACW 3 cut(s) 741, 1023, 1119
TfiI GAWTC 5 cut(s) 224, 428, 658, 970, 1012
Tru1I TTAA 4 cut(s) 492, 540, 678, 1032
Tru9I TTAA 4 cut(s) 492, 540, 678, 1032
TscAI CASTG 1 cut(s) 898
TseFI GTSAC 4 cut(s) 321, 406, 520, 836
TseI GCWGC 3 cut(s) 77, 421, 1132
Tsp45I GTSAC 4 cut(s) 321, 406, 520, 836
TspDTI ATGAA 7 cut(s) 232, 290, 405, 707, 889, 926, 1073
TspGWI ACGGA 1 cut(s) 390
TspRI CASTG 1 cut(s) 898
VpaK11BI GGWCC 1 cut(s) 1055
VspI ATTAAT 1 cut(s) 678
XceI RCATGY 1 cut(s) 91
XmiI GTMKAC 1 cut(s) 366
XmnI GAANNNNTTC 1 cut(s) 239
XspI CTAG 3 cut(s) 590, 663, 1065
Zsp2I ATGCAT 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.