MD09G1039300.v1.1

Zinc finger AN1 domain-containing stress-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011887
Physical Location & Seq
Forward (+)
2400303 .. 2400884
582 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1039300.v1.1.491

Sequence Viewer

Length: 582 bp
ATGATGGGAGGAACAGAAGCTTTTCCGGATTTGGGTCGACACTGCCAGCTCTCCGATTGCCACCAACTCGATTTCCTCCCCTTCCAATGCGACGGTTGCCGTAAGGTTTTCTGCGTTGAGCATCGGTCTTACAAGTCCCACGAGTGCCCCAAATCGGACCACAACAGCAGAAAGGTGGTGGTTTGCGAAATCTGCTCCGCCTCCGTAGAAACCACAGGCTGCGACGGCGAACAAGACCACAAATTGCTGTTGCAGAAGCACGCCAAGTCCGGGAATTGCGACCCCAAAAACAAGAAGAAGCCCACCTGTCCCGTTCGCCGGTGCAAGGAGACTCTCACCTTTTCTAATACCAGCACCTGCAAAACCTGCCAGATCAAGGTCTGCCTCAAGCACCGGTTCCCGGCCGACCATGTTTGCAGGAAGCAAACGGCAGCTCAGCCGTCGTTGCTGGCGGGTAATGGCGTCAGCTGGAACGACAGGTTCATGGCTGCTTTTGCTTCGAGGAAAGGGAAAGAATGCGGGAAGAGTGAGCGGGATTCCAAGTCTTCTGCTTCGAGTGGGCCATCTGTTCGAGCGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0010200 GO:0010243 GO:0033993 GO:0042221 GO:0042493 GO:0050896 GO:0097305 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-AN1

Protein Analysis

194

Amino Acids

21.39

Weight (kDa)

9.04

Isoelectric Point (pI)

44.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-AN1 PF01428 15 - 52 5.1e-12 AN1-like Zinc finger
zf-AN1 PF01428 103 - 142 5.3e-08 AN1-like Zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28210 AT3G28210
fragaria_vesca FvH4_6g50080
malus_domestica MD09G1039300.v1.1 MD17G1040300.v1.1
prunus_persica Prupe.3G278700_v2.0.a1
pyrus_communis pycom17g03610
rosa_chinensis RchiOBHm_Chr2g0170351
rosa_laevigata RLG00000021948
rosa_multiflora Rmu_sc0011601.1_g000011 Rmu_sc0012445.1_g000015
rosa_roxburghii Rroxscaffold_2G00081420
rosa_rugosa Rorug02G0548400
rosa_samantha Rh2AG619800 Rh2BG630700 Rh2CG600800 Rh2DG643600
rosa_wichuraiana Rw2G051340 Rw2G051350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 365
Acc36I ACCTGC 2 cut(s) 365, 374
AccBSI CCGCTC 1 cut(s) 532
AccI GTMKAC 1 cut(s) 37
AccIII TCCGGA 1 cut(s) 25
AciI CCGC 4 cut(s) 198, 452, 519, 532
AcoI YGGCCR 1 cut(s) 402
AcyI GRCGYC 1 cut(s) 462
AfiI CCNNNNNNNGG 7 cut(s) 32, 154, 270, 318, 325, 376, 400
AgeI ACCGGT 1 cut(s) 393
AluBI AGCT 4 cut(s) 20, 49, 434, 468
AluI AGCT 4 cut(s) 20, 49, 434, 468
Alw26I GTCTC 1 cut(s) 323
AlwNI CAGNNNCTG 1 cut(s) 357
Aor13HI TCCGGA 1 cut(s) 25
AoxI GGCC 2 cut(s) 402, 560
ApeKI GCWGC 3 cut(s) 219, 431, 488
AsiGI ACCGGT 1 cut(s) 393
Asp700I GAANNNNTTC 1 cut(s) 21
AspS9I GGNCC 2 cut(s) 157, 560
AsuC2I CCSGG 2 cut(s) 271, 401
AsuHPI GGTGA 1 cut(s) 328
AvaII GGWCC 1 cut(s) 157
BaeGI GKGCMC 1 cut(s) 149
BauI CACGAG 1 cut(s) 140
BbsI GAAGAC 1 cut(s) 537
BbvI GCAGC 3 cut(s) 206, 443, 475
BccI CCATC 1 cut(s) 571
BceAI ACGGC 4 cut(s) 84, 241, 424, 444
BcnI CCSGG 2 cut(s) 271, 401
BcoDI GTCTC 1 cut(s) 323
BfuAI ACCTGC 2 cut(s) 365, 374
BisI GCNGC 3 cut(s) 220, 432, 489
BlpI GCTNAGC 1 cut(s) 435
BlsI GCNGC 3 cut(s) 221, 433, 490
Bme1390I CCNGG 2 cut(s) 271, 401
Bme18I GGWCC 1 cut(s) 157
BmgT120I GGNCC 2 cut(s) 157, 560
BmiI GGNNCC 1 cut(s) 398
BmrFI CCNGG 2 cut(s) 271, 401
BmsI GCATC 1 cut(s) 130
BpiI GAAGAC 1 cut(s) 537
Bpu1102I GCTNAGC 1 cut(s) 435
BpuEI CTTGAG 1 cut(s) 371
BpuMI CCSGG 2 cut(s) 271, 401
BsaHI GRCGYC 1 cut(s) 462
BsaWI WCCGGW 2 cut(s) 25, 393
Bsc4I CCNNNNNNNGG 7 cut(s) 32, 154, 270, 318, 325, 376, 400
Bse118I RCCGGY 2 cut(s) 318, 393
BseAI TCCGGA 1 cut(s) 25
BseLI CCNNNNNNNGG 7 cut(s) 32, 154, 270, 318, 325, 376, 400
BseMII CTCAG 1 cut(s) 449
BseSI GKGCMC 1 cut(s) 149
BseX3I CGGCCG 1 cut(s) 402
BseXI GCAGC 3 cut(s) 206, 443, 475
Bsh1285I CGRYCG 1 cut(s) 405
BshFI GGCC 2 cut(s) 404, 562
BshTI ACCGGT 1 cut(s) 393
BsiEI CGRYCG 1 cut(s) 405
BsiSI CCGG 5 cut(s) 26, 270, 319, 394, 401
BslFI GGGAC 2 cut(s) 121, 294
BslI CCNNNNNNNGG 7 cut(s) 32, 154, 270, 318, 325, 376, 400
BsmAI GTCTC 1 cut(s) 323
BsmFI GGGAC 2 cut(s) 121, 294
BsmI GAATGC 1 cut(s) 521
BsnI GGCC 2 cut(s) 404, 562
Bsp1286I GDGCHC 1 cut(s) 149
Bsp13I TCCGGA 1 cut(s) 25
Bsp143I GATC 1 cut(s) 372
Bsp1720I GCTNAGC 1 cut(s) 435
BspACI CCGC 4 cut(s) 198, 452, 519, 532
BspANI GGCC 2 cut(s) 404, 562
BspCNI CTCAG 1 cut(s) 448
BspEI TCCGGA 1 cut(s) 25
BspLI GGNNCC 1 cut(s) 398
BspMI ACCTGC 2 cut(s) 365, 374
BsrBI CCGCTC 1 cut(s) 532
BsrFI RCCGGY 2 cut(s) 318, 393
BssAI RCCGGY 2 cut(s) 318, 393
BssMI GATC 1 cut(s) 372
BssNI GRCGYC 1 cut(s) 462
BssSI CACGAG 1 cut(s) 140
Bst2BI CACGAG 1 cut(s) 140
Bst4CI ACNGT 1 cut(s) 95
Bst6I CTCTTC 1 cut(s) 518
BstACI GRCGYC 1 cut(s) 462
BstAPI GCANNNNNTGC 1 cut(s) 366
BstC8I GCNNGC 3 cut(s) 47, 261, 450
BstDEI CTNAG 1 cut(s) 435
BstKTI GATC 1 cut(s) 375
BstMAI GTCTC 1 cut(s) 323
BstMBI GATC 1 cut(s) 372
BstMCI CGRYCG 1 cut(s) 405
BstMWI GCNNNNNNNGC 6 cut(s) 96, 192, 225, 366, 445, 494
BstSCI CCNGG 2 cut(s) 269, 399
BstSLI GKGCMC 1 cut(s) 149
BstV1I GCAGC 3 cut(s) 206, 443, 475
BstV2I GAAGAC 1 cut(s) 537
BstZI CGGCCG 1 cut(s) 402
BsuRI GGCC 2 cut(s) 404, 562
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 40
BveI ACCTGC 2 cut(s) 365, 374
Cac8I GCNNGC 3 cut(s) 47, 261, 450
CaiI CAGNNNCTG 1 cut(s) 357
Cfr10I RCCGGY 2 cut(s) 318, 393
Cfr13I GGNCC 2 cut(s) 157, 560
CseI GACGC 1 cut(s) 451
CspAI ACCGGT 1 cut(s) 393
CviAII CATG 2 cut(s) 410, 484
DdeI CTNAG 1 cut(s) 435
DpnI GATC 1 cut(s) 374
DpnII GATC 1 cut(s) 372
EaeI YGGCCR 1 cut(s) 402
EagI CGGCCG 1 cut(s) 402
Eam1104I CTCTTC 1 cut(s) 518
EarI CTCTTC 1 cut(s) 518
EciI GGCGGA 1 cut(s) 187
EclXI CGGCCG 1 cut(s) 402
Eco47I GGWCC 1 cut(s) 157
Eco52I CGGCCG 1 cut(s) 402
FaeI CATG 2 cut(s) 413, 487
FaiI YATR 2 cut(s) 411, 485
FaqI GGGAC 2 cut(s) 121, 294
FatI CATG 2 cut(s) 409, 483
FauI CCCGC 3 cut(s) 445, 512, 525
FblI GTMKAC 1 cut(s) 37
Fnu4HI GCNGC 3 cut(s) 220, 432, 489
Fsp4HI GCNGC 3 cut(s) 220, 432, 489
GluI GCNGC 3 cut(s) 220, 432, 489
HaeIII GGCC 2 cut(s) 404, 562
HapII CCGG 5 cut(s) 26, 270, 319, 394, 401
HgaI GACGC 1 cut(s) 451
Hin1I GRCGYC 1 cut(s) 462
Hin1II CATG 2 cut(s) 413, 487
HincII GTYRAC 1 cut(s) 38
HindII GTYRAC 1 cut(s) 38
HindIII AAGCTT 1 cut(s) 18
HinfI GANTC 2 cut(s) 331, 536
HpaII CCGG 5 cut(s) 26, 270, 319, 394, 401
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 1 cut(s) 38
Hpy188I TCNGA 2 cut(s) 55, 157
Hpy188III TCNNGA 1 cut(s) 26
Hpy8I GTNNAC 1 cut(s) 38
Hpy99I CGWCG 3 cut(s) 95, 227, 445
HpyAV CCTTC 1 cut(s) 91
HpyCH4III ACNGT 1 cut(s) 95
HpyCH4V TGCA 4 cut(s) 253, 324, 360, 417
HpyF10VI GCNNNNNNNGC 6 cut(s) 96, 192, 225, 366, 445, 494
HpyF3I CTNAG 1 cut(s) 435
Hsp92I GRCGYC 1 cut(s) 462
Hsp92II CATG 2 cut(s) 413, 487
Kpn2I TCCGGA 1 cut(s) 25
Kzo9I GATC 1 cut(s) 372
LmnI GCTCC 1 cut(s) 200
Lsp1109I GCAGC 3 cut(s) 206, 443, 475
LweI GCATC 1 cut(s) 130
MalI GATC 1 cut(s) 374
MbiI CCGCTC 1 cut(s) 532
MboI GATC 1 cut(s) 372
MboII GAAGA 3 cut(s) 307, 535, 537
MhlI GDGCHC 1 cut(s) 149
MluCI AATT 2 cut(s) 242, 274
MlyI GAGTC 1 cut(s) 325
MnlI CCTC 4 cut(s) 86, 211, 395, 495
MroI TCCGGA 1 cut(s) 25
MroXI GAANNNNTTC 1 cut(s) 21
MspA1I CMGCKG 1 cut(s) 468
MspI CCGG 5 cut(s) 26, 270, 319, 394, 401
MspR9I CCNGG 2 cut(s) 271, 401
Mva1269I GAATGC 1 cut(s) 521
MwoI GCNNNNNNNGC 6 cut(s) 96, 192, 225, 366, 445, 494
NciI CCSGG 2 cut(s) 271, 401
NdeII GATC 1 cut(s) 372
NlaIII CATG 2 cut(s) 413, 487
NlaIV GGNNCC 1 cut(s) 398
PaqCI CACCTGC 1 cut(s) 365
PctI GAATGC 1 cut(s) 521
PdmI GAANNNNTTC 1 cut(s) 21
PfeI GAWTC 1 cut(s) 536
PfoI TCCNGGA 1 cut(s) 269
PinAI ACCGGT 1 cut(s) 393
PkrI GCNGC 3 cut(s) 221, 433, 490
PleI GAGTC 1 cut(s) 325
PpsI GAGTC 1 cut(s) 325
PspN4I GGNNCC 1 cut(s) 398
PspPI GGNCC 2 cut(s) 157, 560
PstNI CAGNNNCTG 1 cut(s) 357
PvuII CAGCTG 1 cut(s) 468
SalI GTCGAC 1 cut(s) 36
SatI GCNGC 3 cut(s) 220, 432, 489
Sau3AI GATC 1 cut(s) 372
Sau96I GGNCC 2 cut(s) 157, 560
SchI GAGTC 1 cut(s) 325
ScrFI CCNGG 2 cut(s) 271, 401
SduI GDGCHC 1 cut(s) 149
SfaNI GCATC 1 cut(s) 130
SgrAI CRCCGGYG 1 cut(s) 318
SinI GGWCC 1 cut(s) 157
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
Sse9I AATT 2 cut(s) 242, 274
SsiI CCGC 4 cut(s) 198, 452, 519, 532
StyD4I CCNGG 2 cut(s) 269, 399
TaaI ACNGT 1 cut(s) 95
TaqI TCGA 5 cut(s) 37, 69, 500, 554, 571
TaqII GACCGA 1 cut(s) 114
TasI AATT 2 cut(s) 242, 274
TfiI GAWTC 1 cut(s) 536
TscAI CASTG 1 cut(s) 47
TseI GCWGC 3 cut(s) 219, 431, 488
TspDTI ATGAA 1 cut(s) 472
TspGWI ACGGA 1 cut(s) 193
TspRI CASTG 1 cut(s) 47
VpaK11BI GGWCC 1 cut(s) 157
XmiI GTMKAC 1 cut(s) 37
XmnI GAANNNNTTC 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.