MD09G1054600.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011588
Physical Location & Seq
Forward (+)
3626751 .. 3628238
1488 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1054600.v1.1.491

Sequence Viewer

Length: 1488 bp
ATGCCGCTGCCGGGGCAACAACAATGGCAAGCAAGCCTGAGCTTCAGCCTGGCCTACATGGCCCAGAACTGCCTCTCCATGACACACCTCAAGCAAATCCACGGCCGCGCAGTCGTCACAGACCTCCACCACCACGCCATCGTCCTCGCCAAGATGTTCCGTTTCGCCGCCATCTCACCCGCCGGCGACTTAAGCTACGCCCACCGCCTGTTCGACCAAATGCCCCGACCAAACACTTTCTTCTACAATACCCTCATCCGTGGCCACTCTAAAAGCTCGTCTCCTTCGGAATCGGTGGTTCTTTTCAACCAGATGAGGCTTAACTGCGTCGACCCAGATGAGTTTACCTTCACGTTTTTGCTGAAATCGCGGTCAAGGATGAAAATTGATGCTGCTCCGATTGTGGGGTCCGATGAGATTCATGGGATTGCGTTGAAGTTTGGATTTTGCGCGCATTTGTTTGTACAGAACGCATTGATTCATATATATGGGGCGAGAGGGATAACGGTTGCGGCGAGGCGAGTTTTCGATGAGGCGGTGGCTCCTGACGTCGTTTCGTGGTCGGGTTTGGTTATTGCGCATGTGAGAGCTGGGGAATTGGAGTTTGCACGGCAGGTGTTTGATGAGATGCCTGAGAGGGATGTGGTTTCCTGGACTGCTATGATTTCTGCGTATTCCCAAGCAAAGTATTCGAGGGAAGCGCTTGATTTGTTTTGGGAGATGAATCGTGAGGGAGTGATGCCTGATGAAGTCACCATGGTGAGTGTAATCTCAGCGTGTGCAGATTCCGCAGATGTAGAAACGGGGATATGCATCCATCGATATATTGATGAGAACGGGTTTTTGTGGATGGTTTCACTTTGCAATGCGCTGATTGACATGTACGCAAAGTGTGGATGCATGGACCGAGCATGGCAACTGTTCAATAAGATGAGCCGGAAGAGCTTGGTCACTTGGAATTCGATGATTTCAGCATGCGCTAACCATGGCAATGCCGATGATGCATTTGGTTTGTTTGAGTGCATGACCGCTGCTGGTGTTGCACTGGATGGTGTCACCTTTTTAGCCCTTTTAGTTGCGTATACACACAAGGGATTGGTAGATGAAGGGCTCAAACTATTCGAGAGAATGCAAACAGACTATAGAATTGAGGCACGAATTGAGCATTATGGTTGTGTGGTTGATATGTTAGGGAAGGCAGGACGACTAGAGGAAGCATACAGATTGATTAGTAGTATGCCAATTCCGGGCAACGATGTTGTTTGGGGAACACTGCTTGCAGCTTGCAGAACTTATGGGGATGCTGATATGGGGGAGAGGGTGGTGAACAGGCTGTTAGAGTTGAAACCAGATGAAGGGGGATACTACATTCTCCTTCGTGATATTTATGTCGCGGCCGGAAGGACAATGGAAGCAAATGCAACGAGGCAAAGCATGAAGGTTAACGGTGCTAGTAAGACTCCCGGATGCAGCTGGGTGGGAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

496

Amino Acids

55.06

Weight (kDa)

6.02

Isoelectric Point (pI)

35.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 77 - 122 2.1e-09 PPR repeat family
PPR_2 PF13041 213 - 261 2.8e-12 PPR repeat family
PPR PF01535 216 - 246 7.9e-08 PPR repeat
PPR_long PF17177 281 - 411 5.9e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 286 - 315 2.8e-06 PPR repeat family
PPR PF01535 288 - 314 4.6e-06 PPR repeat
PPR_2 PF13041 315 - 358 3.5e-08 PPR repeat family
PPR PF01535 317 - 347 2.6e-08 PPR repeat
E_motif PF20431 432 - 493 2.1e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 604
AatII GACGTC 1 cut(s) 552
Acc16I TGCGCA 1 cut(s) 579
Acc36I ACCTGC 1 cut(s) 604
AccI GTMKAC 2 cut(s) 330, 1082
AccII CGCG 4 cut(s) 108, 370, 452, 1394
AcoI YGGCCR 3 cut(s) 103, 262, 1395
AcsI RAATTY 1 cut(s) 958
AcuI CTGAAG 1 cut(s) 28
AcyI GRCGYC 1 cut(s) 549
AfaI GTAC 2 cut(s) 465, 884
AfeI AGCGCT 1 cut(s) 702
AfiI CCNNNNNNNGG 4 cut(s) 11, 404, 1247, 1355
AflII CTTAAG 1 cut(s) 190
AflIII ACRYGT 1 cut(s) 879
AgsI TTSAA 4 cut(s) 307, 436, 925, 1345
AjnI CCWGG 2 cut(s) 48, 650
AleI CACNNNNGTG 1 cut(s) 758
AloI GAACNNNNNNTCC 2 cut(s) 59, 91
AluBI AGCT 7 cut(s) 42, 195, 276, 590, 945, 1283, 1473
AluI AGCT 7 cut(s) 42, 195, 276, 590, 945, 1283, 1473
Alw26I GTCTC 1 cut(s) 285
Aor51HI AGCGCT 1 cut(s) 702
AoxI GGCC 5 cut(s) 51, 60, 103, 262, 1395
ApeKI GCWGC 5 cut(s) 7, 392, 1031, 1280, 1470
ApoI RAATTY 1 cut(s) 958
AspLEI GCGC 7 cut(s) 110, 452, 454, 580, 703, 871, 980
AspS9I GGNCC 3 cut(s) 61, 408, 904
AsuC2I CCSGG 3 cut(s) 12, 1248, 1464
AsuHPI GGTGA 5 cut(s) 168, 745, 772, 1048, 1336
AvaII GGWCC 2 cut(s) 408, 904
BalI TGGCCA 1 cut(s) 264
BanII GRGCYC 1 cut(s) 1113
BbvI GCAGC 4 cut(s) 379, 1018, 1292, 1482
BccI CCATC 5 cut(s) 146, 179, 825, 844, 1043
BceAI ACGGC 2 cut(s) 118, 626
BcgI CGANNNNNNTGC 2 cut(s) 672, 706
BciT130I CCWGG 2 cut(s) 50, 652
BciVI GTATCC 1 cut(s) 1355
BcnI CCSGG 3 cut(s) 12, 1248, 1464
BcoDI GTCTC 1 cut(s) 285
BfaI CTAG 2 cut(s) 1208, 1452
BfmI CTRYAG 1 cut(s) 1141
BfoI RGCGCY 1 cut(s) 704
BfrI CTTAAG 1 cut(s) 190
BfuAI ACCTGC 1 cut(s) 604
BfuI GTATCC 1 cut(s) 1355
BglI GCCNNNNNGGC 1 cut(s) 59
Bme1390I CCNGG 5 cut(s) 12, 50, 652, 1248, 1464
Bme18I GGWCC 2 cut(s) 408, 904
BmgT120I GGNCC 3 cut(s) 61, 408, 904
BmiI GGNNCC 2 cut(s) 409, 543
BmrFI CCNGG 5 cut(s) 12, 50, 652, 1248, 1464
BmsI GCATC 8 cut(s) 379, 618, 729, 822, 887, 991, 1291, 1457
Bpu10I CCTNAGC 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 74
BpuMI CCSGG 3 cut(s) 12, 1248, 1464
Bsa29I ATCGAT 1 cut(s) 820
BsaBI GATNNNNATC 1 cut(s) 812
BsaHI GRCGYC 1 cut(s) 549
BsaJI CCNNGG 5 cut(s) 11, 100, 259, 756, 985
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 4 cut(s) 11, 404, 1247, 1355
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 1 cut(s) 1050
Bse3DI GCAATG 2 cut(s) 871, 997
Bse8I GATNNNNATC 1 cut(s) 812
BseBI CCWGG 2 cut(s) 50, 652
BseCI ATCGAT 1 cut(s) 820
BseDI CCNNGG 5 cut(s) 11, 100, 259, 756, 985
BseGI GGATG 9 cut(s) 255, 384, 646, 813, 855, 902, 1054, 1306, 1472
BseJI GATNNNNATC 1 cut(s) 812
BseLI CCNNNNNNNGG 4 cut(s) 11, 404, 1247, 1355
BseMI GCAATG 2 cut(s) 871, 997
BseMII CTCAG 3 cut(s) 29, 624, 786
BseNI ACTGG 1 cut(s) 1050
BsePI GCGCGC 1 cut(s) 450
BseX3I CGGCCG 2 cut(s) 103, 1395
BseXI GCAGC 4 cut(s) 379, 1018, 1292, 1482
BseYI CCCAGC 2 cut(s) 590, 1473
BsgI GTGCAG 1 cut(s) 801
Bsh1236I CGCG 4 cut(s) 108, 370, 452, 1394
Bsh1285I CGRYCG 2 cut(s) 106, 1398
BshFI GGCC 5 cut(s) 53, 62, 105, 264, 1397
BshVI ATCGAT 1 cut(s) 820
BsiEI CGRYCG 2 cut(s) 106, 1398
BsiSI CCGG 6 cut(s) 11, 183, 937, 1247, 1398, 1464
BslI CCNNNNNNNGG 4 cut(s) 11, 404, 1247, 1355
BsmAI GTCTC 1 cut(s) 285
BsmBI CGTCTC 1 cut(s) 285
BsmI GAATGC 1 cut(s) 1134
BsnI GGCC 5 cut(s) 53, 62, 105, 264, 1397
Bsp1286I GDGCHC 1 cut(s) 1113
Bsp1407I TGTACA 1 cut(s) 463
Bsp19I CCATGG 2 cut(s) 756, 985
BspANI GGCC 5 cut(s) 53, 62, 105, 264, 1397
BspCNI CTCAG 3 cut(s) 30, 625, 785
BspDI ATCGAT 1 cut(s) 820
BspFNI CGCG 4 cut(s) 108, 370, 452, 1394
BspLI GGNNCC 2 cut(s) 409, 543
BspMI ACCTGC 1 cut(s) 604
BspQI GCTCTTC 1 cut(s) 935
BspTI CTTAAG 1 cut(s) 190
BsrDI GCAATG 2 cut(s) 871, 997
BsrFI RCCGGY 1 cut(s) 182
BsrGI TGTACA 1 cut(s) 463
BsrI ACTGG 1 cut(s) 1050
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 5 cut(s) 11, 100, 259, 756, 985
BssHII GCGCGC 1 cut(s) 450
BssNAI GTATAC 1 cut(s) 1083
BssNI GRCGYC 1 cut(s) 549
BssT1I CCWWGG 2 cut(s) 756, 985
Bst1107I GTATAC 1 cut(s) 1083
Bst2UI CCWGG 2 cut(s) 50, 652
Bst4CI ACNGT 3 cut(s) 508, 921, 1448
Bst6I CTCTTC 1 cut(s) 935
BstACI GRCGYC 1 cut(s) 549
BstAFI CTTAAG 1 cut(s) 190
BstAUI TGTACA 1 cut(s) 463
BstC8I GCNNGC 7 cut(s) 30, 34, 184, 452, 976, 1278, 1285
BstDEI CTNAG 3 cut(s) 38, 633, 772
BstDSI CCRYGG 4 cut(s) 100, 259, 756, 985
BstF5I GGATG 9 cut(s) 255, 384, 646, 813, 855, 902, 1054, 1306, 1472
BstFNI CGCG 4 cut(s) 108, 370, 452, 1394
BstH2I RGCGCY 1 cut(s) 704
BstHHI GCGC 7 cut(s) 110, 452, 454, 580, 703, 871, 980
BstMAI GTCTC 1 cut(s) 285
BstMCI CGRYCG 2 cut(s) 106, 1398
BstMWI GCNNNNNNNGC 8 cut(s) 13, 59, 192, 367, 788, 942, 1001, 1040
BstNI CCWGG 2 cut(s) 50, 652
BstNSI RCATGY 3 cut(s) 584, 883, 978
BstSCI CCNGG 5 cut(s) 10, 48, 650, 1246, 1462
BstSFI CTRYAG 1 cut(s) 1141
BstUI CGCG 4 cut(s) 108, 370, 452, 1394
BstV1I GCAGC 4 cut(s) 379, 1018, 1292, 1482
BstZ17I GTATAC 1 cut(s) 1083
BstZI CGGCCG 2 cut(s) 103, 1395
Bsu15I ATCGAT 1 cut(s) 820
BsuI GTATCC 1 cut(s) 1355
BsuRI GGCC 5 cut(s) 53, 62, 105, 264, 1397
BsuTUI ATCGAT 1 cut(s) 820
BtgI CCRYGG 4 cut(s) 100, 259, 756, 985
BtsCI GGATG 9 cut(s) 255, 384, 646, 813, 855, 902, 1054, 1306, 1472
BtsI GCAGTG 1 cut(s) 1271
BtsIMutI CAGTG 2 cut(s) 1043, 1271
BveI ACCTGC 1 cut(s) 604
Cac8I GCNNGC 7 cut(s) 30, 34, 184, 452, 976, 1278, 1285
CfoI GCGC 7 cut(s) 110, 452, 454, 580, 703, 871, 980
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 3 cut(s) 61, 408, 904
ClaI ATCGAT 1 cut(s) 820
CseI GACGC 1 cut(s) 316
Csp6I GTAC 2 cut(s) 464, 883
CviQI GTAC 2 cut(s) 464, 883
DdeI CTNAG 3 cut(s) 38, 633, 772
EaeI YGGCCR 3 cut(s) 103, 262, 1395
EagI CGGCCG 2 cut(s) 103, 1395
Eam1104I CTCTTC 1 cut(s) 935
EarI CTCTTC 1 cut(s) 935
EclXI CGGCCG 2 cut(s) 103, 1395
Eco130I CCWWGG 2 cut(s) 756, 985
Eco24I GRGCYC 1 cut(s) 1113
Eco47I GGWCC 2 cut(s) 408, 904
Eco47III AGCGCT 1 cut(s) 702
Eco52I CGGCCG 2 cut(s) 103, 1395
Eco57I CTGAAG 1 cut(s) 28
EcoRI GAATTC 1 cut(s) 958
EcoRII CCWGG 2 cut(s) 48, 650
EcoT14I CCWWGG 2 cut(s) 756, 985
EcoT22I ATGCAT 3 cut(s) 815, 902, 1006
EcoT38I GRGCYC 1 cut(s) 1113
ErhI CCWWGG 2 cut(s) 756, 985
Esp3I CGTCTC 1 cut(s) 285
FauI CCCGC 1 cut(s) 187
FblI GTMKAC 2 cut(s) 330, 1082
FokI GGATG 9 cut(s) 242, 391, 653, 800, 862, 909, 1061, 1313, 1479
FriOI GRGCYC 1 cut(s) 1113
FspBI CTAG 2 cut(s) 1208, 1452
FspI TGCGCA 1 cut(s) 579
GlaI GCGC 7 cut(s) 109, 451, 453, 579, 702, 870, 979
GsaI CCCAGC 2 cut(s) 594, 1477
HaeII RGCGCY 1 cut(s) 704
HaeIII GGCC 5 cut(s) 53, 62, 105, 264, 1397
HapII CCGG 6 cut(s) 11, 183, 937, 1247, 1398, 1464
HgaI GACGC 1 cut(s) 316
HhaI GCGC 7 cut(s) 110, 452, 454, 580, 703, 871, 980
Hin1I GRCGYC 1 cut(s) 549
Hin6I GCGC 7 cut(s) 108, 450, 452, 578, 701, 869, 978
HinP1I GCGC 7 cut(s) 108, 450, 452, 578, 701, 869, 978
HincII GTYRAC 2 cut(s) 331, 1444
HindII GTYRAC 2 cut(s) 331, 1444
HinfI GANTC 6 cut(s) 290, 418, 478, 724, 785, 1459
HpaI GTTAAC 1 cut(s) 1444
HpaII CCGG 6 cut(s) 11, 183, 937, 1247, 1398, 1464
HphI GGTGA 5 cut(s) 168, 745, 772, 1048, 1336
Hpy166II GTNNAC 5 cut(s) 331, 345, 1083, 1327, 1444
Hpy188I TCNGA 3 cut(s) 289, 399, 412
Hpy188III TCNNGA 4 cut(s) 545, 728, 1123, 1379
Hpy8I GTNNAC 5 cut(s) 331, 345, 1083, 1327, 1444
Hpy99I CGWCG 2 cut(s) 332, 554
HpyAV CCTTC 8 cut(s) 294, 358, 1100, 1189, 1349, 1385, 1395, 1432
HpyCH4III ACNGT 3 cut(s) 508, 921, 1448
HpyCH4IV ACGT 2 cut(s) 353, 549
HpyF10VI GCNNNNNNNGC 8 cut(s) 13, 59, 192, 367, 788, 942, 1001, 1040
HpyF3I CTNAG 3 cut(s) 38, 633, 772
HpySE526I ACGT 2 cut(s) 353, 549
Hsp92I GRCGYC 1 cut(s) 549
HspAI GCGC 7 cut(s) 108, 450, 452, 578, 701, 869, 978
KroI GCCGGC 1 cut(s) 182
KroNI GCCGGC 1 cut(s) 184
KspAI GTTAAC 1 cut(s) 1444
LguI GCTCTTC 1 cut(s) 935
LmnI GCTCC 3 cut(s) 400, 547, 1481
Lsp1109I GCAGC 4 cut(s) 379, 1018, 1292, 1482
LweI GCATC 8 cut(s) 379, 618, 729, 822, 887, 991, 1291, 1457
MaeI CTAG 2 cut(s) 1208, 1452
MaeII ACGT 2 cut(s) 353, 549
MaeIII GTNAC 4 cut(s) 115, 751, 949, 1054
MboII GAAGA 2 cut(s) 232, 952
MhlI GDGCHC 1 cut(s) 1113
MlsI TGGCCA 1 cut(s) 264
MluCI AATT 6 cut(s) 384, 596, 958, 1146, 1158, 1242
MluNI TGGCCA 1 cut(s) 264
MlyI GAGTC 1 cut(s) 1453
Mox20I TGGCCA 1 cut(s) 264
Mph1103I ATGCAT 3 cut(s) 815, 902, 1006
MreI CGCCGGCG 1 cut(s) 182
MroNI GCCGGC 1 cut(s) 182
MscI TGGCCA 1 cut(s) 264
MseI TTAA 3 cut(s) 191, 321, 1443
MslI CAYNNNNRTG 3 cut(s) 486, 758, 990
Msp20I TGGCCA 1 cut(s) 264
MspA1I CMGCKG 3 cut(s) 7, 1031, 1473
MspCI CTTAAG 1 cut(s) 190
MspI CCGG 6 cut(s) 11, 183, 937, 1247, 1398, 1464
MspR9I CCNGG 5 cut(s) 12, 50, 652, 1248, 1464
Mva1269I GAATGC 1 cut(s) 1134
MvaI CCWGG 2 cut(s) 50, 652
MvnI CGCG 4 cut(s) 108, 370, 452, 1394
MwoI GCNNNNNNNGC 8 cut(s) 13, 59, 192, 367, 788, 942, 1001, 1040
NaeI GCCGGC 1 cut(s) 184
NciI CCSGG 3 cut(s) 12, 1248, 1464
NcoI CCATGG 2 cut(s) 756, 985
NgoMIV GCCGGC 1 cut(s) 182
NlaIV GGNNCC 2 cut(s) 409, 543
NmuCI GTSAC 4 cut(s) 115, 751, 949, 1054
NsbI TGCGCA 1 cut(s) 579
NsiI ATGCAT 3 cut(s) 815, 902, 1006
NspI RCATGY 3 cut(s) 584, 883, 978
OliI CACNNNNGTG 1 cut(s) 758
PaeI GCATGC 1 cut(s) 978
PaqCI CACCTGC 1 cut(s) 604
PauI GCGCGC 1 cut(s) 450
PciI ACATGT 1 cut(s) 879
PciSI GCTCTTC 1 cut(s) 935
PcsI WCGNNNNNNNCGW 1 cut(s) 512
PctI GAATGC 1 cut(s) 1134
PdiI GCCGGC 1 cut(s) 184
PfeI GAWTC 5 cut(s) 290, 418, 478, 724, 785
PfoI TCCNGGA 2 cut(s) 650, 1462
PleI GAGTC 1 cut(s) 1453
PpsI GAGTC 1 cut(s) 1453
PscI ACATGT 1 cut(s) 879
Psp6I CCWGG 2 cut(s) 48, 650
PspFI CCCAGC 2 cut(s) 590, 1473
PspGI CCWGG 2 cut(s) 48, 650
PspN4I GGNNCC 2 cut(s) 409, 543
PspPI GGNCC 3 cut(s) 61, 408, 904
PteI GCGCGC 1 cut(s) 450
PvuII CAGCTG 1 cut(s) 1473
RsaI GTAC 2 cut(s) 465, 884
RsaNI GTAC 2 cut(s) 464, 883
RseI CAYNNNNRTG 3 cut(s) 486, 758, 990
SalI GTCGAC 1 cut(s) 329
SapI GCTCTTC 1 cut(s) 935
SaqAI TTAA 3 cut(s) 191, 321, 1443
Sau96I GGNCC 3 cut(s) 61, 408, 904
SchI GAGTC 1 cut(s) 1453
ScrFI CCNGG 5 cut(s) 12, 50, 652, 1248, 1464
SduI GDGCHC 1 cut(s) 1113
SfaNI GCATC 8 cut(s) 379, 618, 729, 822, 887, 991, 1291, 1457
SfcI CTRYAG 1 cut(s) 1141
SfiI GGCCNNNNNGGCC 1 cut(s) 59
SgrAI CRCCGGYG 1 cut(s) 182
SinI GGWCC 2 cut(s) 408, 904
SmiMI CAYNNNNRTG 3 cut(s) 486, 758, 990
SmlI CTYRAG 2 cut(s) 89, 190
SmoI CTYRAG 2 cut(s) 89, 190
SphI GCATGC 1 cut(s) 978
Sse9I AATT 6 cut(s) 384, 596, 958, 1146, 1158, 1242
SspMI CTAG 2 cut(s) 1208, 1452
StyD4I CCNGG 5 cut(s) 10, 48, 650, 1246, 1462
StyI CCWWGG 2 cut(s) 756, 985
TaaI ACNGT 3 cut(s) 508, 921, 1448
TaiI ACGT 2 cut(s) 356, 552
TaqI TCGA 7 cut(s) 213, 330, 528, 692, 820, 962, 1122
TaqII GACCGA 1 cut(s) 921
TasI AATT 6 cut(s) 384, 596, 958, 1146, 1158, 1242
TatI WGTACW 1 cut(s) 463
TauI GCSGC 5 cut(s) 7, 108, 170, 515, 1397
TfiI GAWTC 5 cut(s) 290, 418, 478, 724, 785
Tru1I TTAA 3 cut(s) 191, 321, 1443
Tru9I TTAA 3 cut(s) 191, 321, 1443
TscAI CASTG 2 cut(s) 1050, 1278
TseFI GTSAC 4 cut(s) 115, 751, 949, 1054
TseI GCWGC 5 cut(s) 7, 392, 1031, 1280, 1470
Tsp45I GTSAC 4 cut(s) 115, 751, 949, 1054
TspDTI ATGAA 8 cut(s) 395, 410, 470, 737, 762, 1119, 1368, 1451
TspGWI ACGGA 2 cut(s) 149, 248
TspRI CASTG 2 cut(s) 1050, 1278
Vha464I CTTAAG 1 cut(s) 190
VpaK11BI GGWCC 2 cut(s) 408, 904
XapI RAATTY 1 cut(s) 958
XceI RCATGY 3 cut(s) 584, 883, 978
XmiI GTMKAC 2 cut(s) 330, 1082
XspI CTAG 2 cut(s) 1208, 1452
ZraI GACGTC 1 cut(s) 550
Zsp2I ATGCAT 3 cut(s) 815, 902, 1006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.