Rw2G049650

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0011588
Physical Location & Seq
Reverse (-)
77255761 .. 77257224
1464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G049650.1

Sequence Viewer

Length: 1464 bp
ATGCAACAGCAATGGCAAGCAAGCCTGAGCTCCAGGCTCGCTTCCATGGCCCAGACCTGCCTCTCCATGAAACAACTCAAGCAAATCCACGCCCGTGCAATCCTTTCAAATCTCCACAACCACGCCATCGTTCTCGGCAAGATGTTCCGCTTCGCCGCGGTCTCACCCTCCGGTGACTTAAACTATGCCCACCAACTGTTCGATCAAATGCCCCAGCCAACTACTTTCTTCTACAACCTCCTCATCCGGGGTCACTCCAAAAGCTCGTCGCCTTGGCATTCGGTGCTTCTATTCAACCAGATGAGACTAAACTCAATCGACCCAGATGAGTTTACCTTCACATTTTTGCTGAAATCTCGTTCGAGGATGAAGATGAATCTCGAAAATGATGAGATACATGGGGCTGTGTTGAAGTCTGGGCTTTCCTTGCATTTGTTTGTGCAGAATTCGTTGATTCATTTGTATGCTGGAAGAGGGATTCCAAGTGCGGCTAGGAGAGTGTTCGATGAGGCTGTGGCTGCTGATGTTGTGTCGTGGTCGGGTTTGGTTATTGCTCATGTGAGAGCCAATGAATTGGATTTTGCACGGCGGGTCTTTGATGAAATGCCTGAGAGGGATGTTGTTTCGTGGACCGCTATGATATCTGCGTATTCACAAGCTAAGTATTCGAGGGAAGCGCTTGGGTTGTTTTGGGAGATGAATCGTGAGGGAGTGAGACCCGACGAGGTCACCATGGTGAGTGTAATCTCAGCATGCACGGATTTAGGAGATGTTCAAACCGGGATAAGCATCCACCGGTTTATTGAGGAGAATGGGTTTGCGTGGATGGTTTCGCTTTGCAATGCACTGATTGATATGTATGCAAAGTGTGGATGCATGGACCGAGCATGGCAACTGTTTGATAGCATGGACCGAAAGAGCTTGATTACTTGGAATTCTATGATTTCAGCATGTGCAAACCATGGCAATGCTGATAATGCATTTGGGTTGTTTGAGTGTATGGTTAGTGCTGGTGTTCCACCTGATGGGGTCACATTCTTGGCCCTTTTAGTTGCTTATACACACAAGGGATTGGTAGATGAAGGGCTCAGACTATTTGAGAGAATGCAAAGGGAGTACAGAATCGAGGCTCGCATTGAGCATTATGGGTGCATGGCAGATATGTTAGGCAAGGCAGGGCGATTGGAGGAAGCATACAGATTAATTAGTAGTATGCCGATTCCAGGCAATGATGTTGTTTGGGGAACACTGCTTGCAGCTTGCAGAACTTATGGCGATGTTGATATGGGCGAGAGGGTTGTAAAGAGGCTAATAAAGCTGAAACCTGATGAAGGTGGGTACTATATTCTCCTTCGTGATATTTATGTTGCAGCAGGAAGGACGGCAGAAGCAAACGATATGAGGCAAACGATGATGGATAATGGTGCTAGTAAGACTCCTGGACGCAGTTGGGTTGGAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

487

Amino Acids

54.67

Weight (kDa)

6.71

Isoelectric Point (pI)

40.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 73 - 118 4.1e-07 PPR repeat family
PPR_2 PF13041 205 - 252 2.2e-11 PPR repeat family
PPR PF01535 208 - 238 1.7e-07 PPR repeat
PPR_long PF17177 273 - 403 1.1e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 307 - 350 1.6e-09 PPR repeat family
PPR PF01535 309 - 339 2.8e-08 PPR repeat
E_motif PF20431 424 - 485 1.8e-10 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 65
AccB7I CCANNNNNTGG 1 cut(s) 1025
AccII CGCG 1 cut(s) 158
AciI CCGC 6 cut(s) 148, 156, 158, 488, 589, 633
AcsI RAATTY 2 cut(s) 445, 934
AfaI GTAC 2 cut(s) 1118, 1340
AfeI AGCGCT 1 cut(s) 678
AfiI CCNNNNNNNGG 4 cut(s) 247, 1025, 1223, 1331
AgeI ACCGGT 1 cut(s) 795
AgsI TTSAA 4 cut(s) 108, 295, 412, 776
AjnI CCWGG 3 cut(s) 32, 1222, 1438
AleI CACNNNNGTG 2 cut(s) 93, 734
AluBI AGCT 6 cut(s) 30, 264, 659, 921, 1259, 1318
AluI AGCT 6 cut(s) 30, 264, 659, 921, 1259, 1318
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 3 cut(s) 166, 298, 709
Aor51HI AGCGCT 1 cut(s) 678
AoxI GGCC 2 cut(s) 48, 1041
ApeKI GCWGC 3 cut(s) 518, 1256, 1370
ApoI RAATTY 2 cut(s) 445, 934
AseI ATTAAT 1 cut(s) 1202
AsiGI ACCGGT 1 cut(s) 795
AspLEI GCGC 1 cut(s) 679
AspS9I GGNCC 5 cut(s) 49, 630, 880, 910, 1042
AsuC2I CCSGG 2 cut(s) 248, 781
AsuHPI GGTGA 4 cut(s) 156, 185, 721, 748
AvaII GGWCC 3 cut(s) 630, 880, 910
BanII GRGCYC 2 cut(s) 32, 1089
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 3 cut(s) 505, 1268, 1382
BccI CCATC 4 cut(s) 134, 820, 1019, 1408
BceAI ACGGC 2 cut(s) 602, 1398
BciT130I CCWGG 3 cut(s) 34, 1224, 1440
BcnI CCSGG 2 cut(s) 248, 781
BcoDI GTCTC 3 cut(s) 166, 298, 709
BfaI CTAG 2 cut(s) 492, 1428
BfoI RGCGCY 1 cut(s) 680
BfuAI ACCTGC 1 cut(s) 65
BisI GCNGC 5 cut(s) 156, 489, 519, 1257, 1371
BlsI GCNGC 5 cut(s) 157, 490, 520, 1258, 1372
Bme1390I CCNGG 5 cut(s) 34, 248, 781, 1224, 1440
Bme18I GGWCC 3 cut(s) 630, 880, 910
BmgT120I GGNCC 5 cut(s) 49, 630, 880, 910, 1042
BmrFI CCNGG 5 cut(s) 34, 248, 781, 1224, 1440
BmsI GCATC 2 cut(s) 798, 863
BpmI CTGGAG 1 cut(s) 16
Bpu10I CCTNAGC 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 62
BpuMI CCSGG 2 cut(s) 248, 781
BsaBI GATNNNNATC 1 cut(s) 788
BsaI GGTCTC 2 cut(s) 166, 709
BsaJI CCNNGG 6 cut(s) 45, 156, 247, 272, 732, 961
BsaWI WCCGGW 2 cut(s) 170, 795
BsaXI ACNNNNNCTCC 2 cut(s) 800, 830
Bsc4I CCNNNNNNNGG 4 cut(s) 247, 1025, 1223, 1331
Bse118I RCCGGY 1 cut(s) 795
Bse3DI GCAATG 4 cut(s) 17, 847, 973, 1234
Bse8I GATNNNNATC 1 cut(s) 788
BseBI CCWGG 3 cut(s) 34, 1224, 1440
BseDI CCNNGG 6 cut(s) 45, 156, 247, 272, 732, 961
BseGI GGATG 6 cut(s) 243, 372, 622, 789, 831, 878
BseJI GATNNNNATC 1 cut(s) 788
BseLI CCNNNNNNNGG 4 cut(s) 247, 1025, 1223, 1331
BseMI GCAATG 4 cut(s) 17, 847, 973, 1234
BseMII CTCAG 4 cut(s) 17, 600, 762, 1102
BseRI GAGGAG 2 cut(s) 230, 821
BseXI GCAGC 3 cut(s) 505, 1268, 1382
BseYI CCCAGC 1 cut(s) 213
BsgI GTGCAG 1 cut(s) 461
Bsh1236I CGCG 1 cut(s) 158
BshFI GGCC 2 cut(s) 50, 1043
BshTI ACCGGT 1 cut(s) 795
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 4 cut(s) 171, 247, 780, 796
BslI CCNNNNNNNGG 4 cut(s) 247, 1025, 1223, 1331
BsmAI GTCTC 3 cut(s) 166, 298, 709
BsmI GAATGC 2 cut(s) 277, 1110
BsnI GGCC 2 cut(s) 50, 1043
Bso31I GGTCTC 2 cut(s) 166, 709
Bsp1286I GDGCHC 2 cut(s) 32, 1089
Bsp143I GATC 1 cut(s) 202
Bsp19I CCATGG 3 cut(s) 45, 732, 961
BspACI CCGC 6 cut(s) 148, 156, 158, 488, 589, 633
BspANI GGCC 2 cut(s) 50, 1043
BspCNI CTCAG 4 cut(s) 18, 601, 761, 1101
BspFNI CGCG 1 cut(s) 158
BspMI ACCTGC 1 cut(s) 65
BspTNI GGTCTC 2 cut(s) 166, 709
BsrDI GCAATG 4 cut(s) 17, 847, 973, 1234
BsrFI RCCGGY 1 cut(s) 795
BssAI RCCGGY 1 cut(s) 795
BssECI CCNNGG 6 cut(s) 45, 156, 247, 272, 732, 961
BssMI GATC 1 cut(s) 202
BssT1I CCWWGG 4 cut(s) 45, 272, 732, 961
Bst2UI CCWGG 3 cut(s) 34, 1224, 1440
Bst4CI ACNGT 2 cut(s) 198, 897
Bst6I CTCTTC 1 cut(s) 466
BstAPI GCANNNNNTGC 1 cut(s) 283
BstC8I GCNNGC 7 cut(s) 18, 22, 39, 754, 1132, 1254, 1261
BstDEI CTNAG 5 cut(s) 26, 609, 660, 748, 1088
BstDSI CCRYGG 4 cut(s) 45, 156, 732, 961
BstEII GGTNACC 1 cut(s) 727
BstENI CCTNNNNNAGG 1 cut(s) 1329
BstF5I GGATG 6 cut(s) 243, 372, 622, 789, 831, 878
BstFNI CGCG 1 cut(s) 158
BstH2I RGCGCY 1 cut(s) 680
BstHHI GCGC 1 cut(s) 679
BstKTI GATC 1 cut(s) 205
BstMAI GTCTC 3 cut(s) 166, 298, 709
BstMBI GATC 1 cut(s) 202
BstMWI GCNNNNNNNGC 6 cut(s) 47, 283, 427, 518, 977, 1315
BstNI CCWGG 3 cut(s) 34, 1224, 1440
BstNSI RCATGY 2 cut(s) 756, 954
BstPI GGTNACC 1 cut(s) 727
BstSCI CCNGG 5 cut(s) 32, 246, 779, 1222, 1438
BstUI CGCG 1 cut(s) 158
BstV1I GCAGC 3 cut(s) 505, 1268, 1382
BstXI CCANNNNNNTGG 1 cut(s) 574
BsuRI GGCC 2 cut(s) 50, 1043
BtgI CCRYGG 4 cut(s) 45, 156, 732, 961
BtgZI GCGATG 1 cut(s) 1290
BtsCI GGATG 6 cut(s) 243, 372, 622, 789, 831, 878
BtsI GCAGTG 1 cut(s) 1247
BtsIMutI CAGTG 2 cut(s) 845, 1247
BveI ACCTGC 1 cut(s) 65
Cac8I GCNNGC 7 cut(s) 18, 22, 39, 754, 1132, 1254, 1261
CfoI GCGC 1 cut(s) 679
Cfr10I RCCGGY 1 cut(s) 795
Cfr13I GGNCC 5 cut(s) 49, 630, 880, 910, 1042
Cfr42I CCGCGG 1 cut(s) 159
CseI GACGC 1 cut(s) 1452
Csp6I GTAC 2 cut(s) 1117, 1339
CspAI ACCGGT 1 cut(s) 795
CviQI GTAC 2 cut(s) 1117, 1339
DdeI CTNAG 5 cut(s) 26, 609, 660, 748, 1088
DpnI GATC 1 cut(s) 204
DpnII GATC 1 cut(s) 202
Eam1104I CTCTTC 1 cut(s) 466
EarI CTCTTC 1 cut(s) 466
Ecl136II GAGCTC 1 cut(s) 30
Eco130I CCWWGG 4 cut(s) 45, 272, 732, 961
Eco24I GRGCYC 2 cut(s) 32, 1089
Eco31I GGTCTC 2 cut(s) 166, 709
Eco32I GATATC 1 cut(s) 642
Eco47I GGWCC 3 cut(s) 630, 880, 910
Eco47III AGCGCT 1 cut(s) 678
Eco53kI GAGCTC 1 cut(s) 30
Eco91I GGTNACC 1 cut(s) 727
EcoICRI GAGCTC 1 cut(s) 30
EcoNI CCTNNNNNAGG 1 cut(s) 1329
EcoO65I GGTNACC 1 cut(s) 727
EcoRI GAATTC 2 cut(s) 445, 934
EcoRII CCWGG 3 cut(s) 32, 1222, 1438
EcoRV GATATC 1 cut(s) 642
EcoT14I CCWWGG 4 cut(s) 45, 272, 732, 961
EcoT22I ATGCAT 2 cut(s) 878, 982
EcoT38I GRGCYC 2 cut(s) 32, 1089
ErhI CCWWGG 4 cut(s) 45, 272, 732, 961
FauI CCCGC 1 cut(s) 582
Fnu4HI GCNGC 5 cut(s) 156, 489, 519, 1257, 1371
FokI GGATG 6 cut(s) 230, 379, 629, 776, 838, 885
FriOI GRGCYC 2 cut(s) 32, 1089
Fsp4HI GCNGC 5 cut(s) 156, 489, 519, 1257, 1371
FspBI CTAG 2 cut(s) 492, 1428
GlaI GCGC 1 cut(s) 678
GluI GCNGC 5 cut(s) 156, 489, 519, 1257, 1371
GsaI CCCAGC 1 cut(s) 217
GsuI CTGGAG 1 cut(s) 16
HaeII RGCGCY 1 cut(s) 680
HaeIII GGCC 2 cut(s) 50, 1043
HapII CCGG 4 cut(s) 171, 247, 780, 796
HgaI GACGC 1 cut(s) 1452
HhaI GCGC 1 cut(s) 679
Hin6I GCGC 1 cut(s) 677
HinP1I GCGC 1 cut(s) 677
HinfI GANTC 7 cut(s) 376, 454, 478, 700, 1122, 1219, 1435
HpaII CCGG 4 cut(s) 171, 247, 780, 796
HphI GGTGA 4 cut(s) 156, 185, 721, 748
Hpy166II GTNNAC 2 cut(s) 333, 630
Hpy188I TCNGA 1 cut(s) 1091
Hpy188III TCNNGA 3 cut(s) 380, 704, 1355
Hpy8I GTNNAC 2 cut(s) 333, 630
Hpy99I CGWCG 2 cut(s) 271, 725
HpyAV CCTTC 5 cut(s) 346, 1076, 1325, 1361, 1371
HpyCH4III ACNGT 2 cut(s) 198, 897
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 283, 427, 518, 977, 1315
HpyF3I CTNAG 5 cut(s) 26, 609, 660, 748, 1088
HspAI GCGC 1 cut(s) 677
KspI CCGCGG 1 cut(s) 159
Kzo9I GATC 1 cut(s) 202
LmnI GCTCC 2 cut(s) 35, 1457
Lsp1109I GCAGC 3 cut(s) 505, 1268, 1382
LweI GCATC 2 cut(s) 798, 863
MaeI CTAG 2 cut(s) 492, 1428
MaeIII GTNAC 4 cut(s) 173, 251, 727, 1030
MalI GATC 1 cut(s) 204
MboI GATC 1 cut(s) 202
MboII GAAGA 3 cut(s) 220, 382, 483
MhlI GDGCHC 2 cut(s) 32, 1089
MluCI AATT 4 cut(s) 445, 572, 934, 1203
MlyI GAGTC 1 cut(s) 1429
MmeI TCCRAC 1 cut(s) 1435
Mph1103I ATGCAT 2 cut(s) 878, 982
MseI TTAA 2 cut(s) 179, 1202
MslI CAYNNNNRTG 4 cut(s) 93, 462, 734, 966
MspA1I CMGCKG 1 cut(s) 158
MspI CCGG 4 cut(s) 171, 247, 780, 796
MspR9I CCNGG 5 cut(s) 34, 248, 781, 1224, 1440
Mva1269I GAATGC 2 cut(s) 277, 1110
MvaI CCWGG 3 cut(s) 34, 1224, 1440
MvnI CGCG 1 cut(s) 158
MwoI GCNNNNNNNGC 6 cut(s) 47, 283, 427, 518, 977, 1315
NciI CCSGG 2 cut(s) 248, 781
NcoI CCATGG 3 cut(s) 45, 732, 961
NdeII GATC 1 cut(s) 202
NmeAIII GCCGAG 1 cut(s) 114
NmuCI GTSAC 4 cut(s) 173, 251, 727, 1030
NsiI ATGCAT 2 cut(s) 878, 982
NspI RCATGY 2 cut(s) 756, 954
OliI CACNNNNGTG 2 cut(s) 93, 734
PaeI GCATGC 1 cut(s) 756
PctI GAATGC 2 cut(s) 277, 1110
PfeI GAWTC 6 cut(s) 376, 454, 478, 700, 1122, 1219
PflFI GACNNNGTC 1 cut(s) 725
PflMI CCANNNNNTGG 1 cut(s) 1025
PfoI TCCNGGA 1 cut(s) 1438
PinAI ACCGGT 1 cut(s) 795
PkrI GCNGC 5 cut(s) 157, 490, 520, 1258, 1372
PleI GAGTC 1 cut(s) 1429
PpsI GAGTC 1 cut(s) 1429
PshBI ATTAAT 1 cut(s) 1202
Psp124BI GAGCTC 1 cut(s) 32
Psp6I CCWGG 3 cut(s) 32, 1222, 1438
PspEI GGTNACC 1 cut(s) 727
PspFI CCCAGC 1 cut(s) 213
PspGI CCWGG 3 cut(s) 32, 1222, 1438
PspPI GGNCC 5 cut(s) 49, 630, 880, 910, 1042
PsyI GACNNNGTC 1 cut(s) 725
RsaI GTAC 2 cut(s) 1118, 1340
RsaNI GTAC 2 cut(s) 1117, 1339
RseI CAYNNNNRTG 4 cut(s) 93, 462, 734, 966
SacI GAGCTC 1 cut(s) 32
SacII CCGCGG 1 cut(s) 159
SaqAI TTAA 2 cut(s) 179, 1202
SatI GCNGC 5 cut(s) 156, 489, 519, 1257, 1371
Sau3AI GATC 1 cut(s) 202
Sau96I GGNCC 5 cut(s) 49, 630, 880, 910, 1042
SchI GAGTC 1 cut(s) 1429
ScrFI CCNGG 5 cut(s) 34, 248, 781, 1224, 1440
SduI GDGCHC 2 cut(s) 32, 1089
SfaNI GCATC 2 cut(s) 798, 863
Sfr303I CCGCGG 1 cut(s) 159
SgrBI CCGCGG 1 cut(s) 159
SinI GGWCC 3 cut(s) 630, 880, 910
SmiMI CAYNNNNRTG 4 cut(s) 93, 462, 734, 966
SmlI CTYRAG 1 cut(s) 77
SmoI CTYRAG 1 cut(s) 77
SphI GCATGC 1 cut(s) 756
Sse9I AATT 4 cut(s) 445, 572, 934, 1203
SsiI CCGC 6 cut(s) 148, 156, 158, 488, 589, 633
SspMI CTAG 2 cut(s) 492, 1428
SstI GAGCTC 1 cut(s) 32
StyD4I CCNGG 5 cut(s) 32, 246, 779, 1222, 1438
StyI CCWWGG 4 cut(s) 45, 272, 732, 961
TaaI ACNGT 2 cut(s) 198, 897
TaqI TCGA 7 cut(s) 201, 318, 362, 381, 504, 668, 1125
TaqII GACCGA 2 cut(s) 897, 927
TasI AATT 4 cut(s) 445, 572, 934, 1203
TatI WGTACW 1 cut(s) 1116
TauI GCSGC 2 cut(s) 158, 491
TfiI GAWTC 6 cut(s) 376, 454, 478, 700, 1122, 1219
Tru1I TTAA 2 cut(s) 179, 1202
Tru9I TTAA 2 cut(s) 179, 1202
TscAI CASTG 2 cut(s) 852, 1254
TseFI GTSAC 4 cut(s) 173, 251, 727, 1030
TseI GCWGC 3 cut(s) 518, 1256, 1370
Tsp45I GTSAC 4 cut(s) 173, 251, 727, 1030
TspDTI ATGAA 9 cut(s) 83, 383, 389, 446, 585, 615, 713, 1095, 1344
TspGWI ACGGA 1 cut(s) 773
TspRI CASTG 2 cut(s) 852, 1254
Tth111I GACNNNGTC 1 cut(s) 725
Van91I CCANNNNNTGG 1 cut(s) 1025
VpaK11BI GGWCC 3 cut(s) 630, 880, 910
VspI ATTAAT 1 cut(s) 1202
XagI CCTNNNNNAGG 1 cut(s) 1329
XapI RAATTY 2 cut(s) 445, 934
XceI RCATGY 2 cut(s) 756, 954
XspI CTAG 2 cut(s) 492, 1428
Zsp2I ATGCAT 2 cut(s) 878, 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.