RLG00000021730

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011588
Physical Location & Seq
Forward (+)
77592175 .. 77593873
1699 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021730

Sequence Viewer

Length: 1578 bp
ATGCAACAGCAATGGCAAGCAAGCCTGAGCTCCAGGCTCGCTTCCATGGCCCAGACCTGCCTCTCCATGAAACAACTCAAGCAAATCCACGCCCGCGCAATCCTTTCAAATCTCCACAACCACACCATCGTTCTCGGCAAGATGTTCCGTTTCGCCGCGGTCTCACCCTCCGGTGACTTAAACTATGCCCACCAACTGTTCGATCAAATGCCCCAACCAACTACTTTCTTCTACAACCTCCTCATACGGGGTTACTCTAAAAGCTCGTCGCCTTGGCATTCGGTGCTTCTATTCAACCAGATGAGACTAAACTCAATCTACCCAGATGAGTTTACCTTCACATTTTTGCTGAAATCTCGTTCGAGGATGAAGATGAATATCGAAAATGATGAGATACATGGGGCTGTGTTGAAGTCTGGTTTTTCCCTGCATTTGTTTGTGCAGAATTCGTTGATTCATTTGTATGCGGGGAGAGGGATTCCCAGTGCGGCTAGGAGAGTGTTCGACGAGGCTGTGGCTGCTGATGTAGTGTCGTGGTCGGGTTTGGTTATTGCTTATGTGAAAGCCGATGAACTGAATTTTGCACGGCGGCTGTTTGATGAAATGCCTGAGAGGGATGTTGTTTCGTGGACCGCTATGATATCTGGGTATTCACAAGCTAAGTATTCGAGGGAAGCGCTTGAGTTGTTTTGGGAGATGAACCGTGAGGGAGTGAGACCGGATGAGGTTACCATGGTGAGTATAATCTCAGCTTGCACTGATTTAGGAGATGTTCAAACCGGGATTAGCATCCACCGATTTATTGAGGAGAATGGGTTTGCGTGGATGGTTTCGCTTTGCAATGCGCTGATTGATATGTATGCAAAGTGTGGATGCATGAACCGAGCATGGCAACTGTTTGATAGCATGGACCGAAAGAGCTTGATTACTTGGAATTCTATGATTTCAGCATGTGCAAACCATGGCAATGCTGATGATGCATTTGGGTTGTTTGAGTGTATGGTTAGTGCTGGTGTTCCACCTGATGGGGTCACATTCTTGGCCCTTTTAGTTGCTTATACACACAAGGGATTGGTAGATGAAGGGCTCAGACTATTTGAGAGAATGCAAAGGGAGTACAGAATCGAGGCTCGCATTGAGCATTATGGGTGTGTGGCAGATATGTTAGGCAAGGCAGGGCGATTGGAGGAAGCATACAGATTAATTAGTAGTATGCCGATTCCGGGGAATGATGTTGTTGGGGGAACACTGCTTGCAGCTTGCAGAACTTATGGCGATGTTGATATGGGCGAGAGGGTTGTAAAGAGGCTAATAGAGCTGAAACCGGATGAAGGTGGGTACTATATTCTCCTTGGTGATATTTATGTTGCAGCAGGATGGACTGCAGAAGCAAACGATATGAGGCAAACGATGATGGATAATGGTGCTAGTAAGACTCCTGGACGCACTAGCATATGTGGAAAACGGAAGACCAAGGACATCCGGATATTAAACCAAGATTCAGGACTTCACTATCAAACAGGAGAAGCAGATTCGGGATGCATTGGTCATCAGACATTTCAATGCAGAGCTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

526

Amino Acids

58.84

Weight (kDa)

6.41

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 73 - 118 1.8e-07 PPR repeat family
PPR_2 PF13041 205 - 252 2.2e-12 PPR repeat family
PPR PF01535 208 - 238 3.2e-08 PPR repeat
PPR_long PF17177 273 - 402 4.2e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 307 - 350 6.1e-10 PPR repeat family
PPR PF01535 309 - 339 9.6e-09 PPR repeat
E_motif PF20431 424 - 485 2.6e-09 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 65
AccB7I CCANNNNNTGG 1 cut(s) 1025
AccII CGCG 2 cut(s) 96, 158
AccIII TCCGGA 1 cut(s) 1482
AciI CCGC 7 cut(s) 94, 156, 158, 467, 488, 589, 633
AcsI RAATTY 3 cut(s) 445, 577, 934
AfaI GTAC 2 cut(s) 1118, 1340
AfeI AGCGCT 1 cut(s) 678
AfiI CCNNNNNNNGG 4 cut(s) 247, 1025, 1223, 1331
AgsI TTSAA 5 cut(s) 108, 295, 412, 776, 1562
AjnI CCWGG 2 cut(s) 32, 1438
AluBI AGCT 8 cut(s) 30, 264, 659, 752, 921, 1259, 1318, 1571
AluI AGCT 8 cut(s) 30, 264, 659, 752, 921, 1259, 1318, 1571
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 3 cut(s) 166, 298, 709
Aor13HI TCCGGA 1 cut(s) 1482
Aor51HI AGCGCT 1 cut(s) 678
AoxI GGCC 2 cut(s) 48, 1041
ApeKI GCWGC 3 cut(s) 518, 1256, 1370
ApoI RAATTY 3 cut(s) 445, 577, 934
AseI ATTAAT 1 cut(s) 1202
AspLEI GCGC 3 cut(s) 98, 679, 847
AspS9I GGNCC 4 cut(s) 49, 630, 910, 1042
AsuC2I CCSGG 2 cut(s) 781, 1224
AsuHPI GGTGA 4 cut(s) 156, 185, 748, 1367
AvaII GGWCC 2 cut(s) 630, 910
BanII GRGCYC 2 cut(s) 32, 1089
BbsI GAAGAC 1 cut(s) 1475
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 3 cut(s) 505, 1268, 1382
BccI CCATC 5 cut(s) 134, 820, 1019, 1371, 1408
BceAI ACGGC 1 cut(s) 602
BciT130I CCWGG 2 cut(s) 34, 1440
BcnI CCSGG 2 cut(s) 781, 1224
BcoDI GTCTC 3 cut(s) 166, 298, 709
BfaI CTAG 3 cut(s) 492, 1428, 1449
BfmI CTRYAG 1 cut(s) 1383
BfoI RGCGCY 1 cut(s) 680
BfuAI ACCTGC 1 cut(s) 65
BisI GCNGC 6 cut(s) 156, 489, 519, 590, 1257, 1371
BlsI GCNGC 6 cut(s) 157, 490, 520, 591, 1258, 1372
Bme1390I CCNGG 4 cut(s) 34, 781, 1224, 1440
Bme18I GGWCC 2 cut(s) 630, 910
BmgT120I GGNCC 4 cut(s) 49, 630, 910, 1042
BmrFI CCNGG 4 cut(s) 34, 781, 1224, 1440
BmrI ACTGGG 1 cut(s) 477
BmsI GCATC 4 cut(s) 798, 863, 967, 1529
BmuI ACTGGG 1 cut(s) 477
BpiI GAAGAC 1 cut(s) 1475
BpmI CTGGAG 1 cut(s) 16
Bpu10I CCTNAGC 1 cut(s) 26
BpuEI CTTGAG 2 cut(s) 62, 701
BpuMI CCSGG 2 cut(s) 781, 1224
BsaBI GATNNNNATC 2 cut(s) 377, 788
BsaI GGTCTC 2 cut(s) 166, 709
BsaJI CCNNGG 8 cut(s) 45, 156, 272, 732, 961, 1223, 1351, 1473
BsaWI WCCGGW 4 cut(s) 170, 718, 1324, 1482
BsaXI ACNNNNNCTCC 2 cut(s) 800, 830
Bsc4I CCNNNNNNNGG 4 cut(s) 247, 1025, 1223, 1331
Bse1I ACTGG 1 cut(s) 483
Bse3DI GCAATG 3 cut(s) 17, 847, 973
Bse8I GATNNNNATC 2 cut(s) 377, 788
BseAI TCCGGA 1 cut(s) 1482
BseBI CCWGG 2 cut(s) 34, 1440
BseDI CCNNGG 8 cut(s) 45, 156, 272, 732, 961, 1223, 1351, 1473
BseJI GATNNNNATC 2 cut(s) 377, 788
BseLI CCNNNNNNNGG 4 cut(s) 247, 1025, 1223, 1331
BseMI GCAATG 3 cut(s) 17, 847, 973
BseMII CTCAG 4 cut(s) 17, 600, 762, 1102
BseNI ACTGG 1 cut(s) 483
BseRI GAGGAG 2 cut(s) 230, 821
BseXI GCAGC 3 cut(s) 505, 1268, 1382
BsgI GTGCAG 1 cut(s) 461
Bsh1236I CGCG 2 cut(s) 96, 158
BshFI GGCC 2 cut(s) 50, 1043
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 6 cut(s) 171, 719, 780, 1223, 1325, 1483
BslI CCNNNNNNNGG 4 cut(s) 247, 1025, 1223, 1331
BsmAI GTCTC 3 cut(s) 166, 298, 709
BsmI GAATGC 2 cut(s) 277, 1110
BsnI GGCC 2 cut(s) 50, 1043
Bso31I GGTCTC 2 cut(s) 166, 709
Bsp1286I GDGCHC 2 cut(s) 32, 1089
Bsp13I TCCGGA 1 cut(s) 1482
Bsp143I GATC 1 cut(s) 202
Bsp19I CCATGG 3 cut(s) 45, 732, 961
BspACI CCGC 7 cut(s) 94, 156, 158, 467, 488, 589, 633
BspANI GGCC 2 cut(s) 50, 1043
BspCNI CTCAG 4 cut(s) 18, 601, 761, 1101
BspEI TCCGGA 1 cut(s) 1482
BspFNI CGCG 2 cut(s) 96, 158
BspMAI CTGCAG 1 cut(s) 1387
BspMI ACCTGC 1 cut(s) 65
BspTNI GGTCTC 2 cut(s) 166, 709
BsrDI GCAATG 3 cut(s) 17, 847, 973
BsrI ACTGG 1 cut(s) 483
BssECI CCNNGG 8 cut(s) 45, 156, 272, 732, 961, 1223, 1351, 1473
BssMI GATC 1 cut(s) 202
BssT1I CCWWGG 6 cut(s) 45, 272, 732, 961, 1351, 1473
Bst2UI CCWGG 2 cut(s) 34, 1440
Bst4CI ACNGT 3 cut(s) 198, 704, 897
BstAPI GCANNNNNTGC 1 cut(s) 283
BstC8I GCNNGC 8 cut(s) 18, 22, 39, 94, 754, 1132, 1254, 1261
BstDEI CTNAG 5 cut(s) 26, 609, 660, 748, 1088
BstDSI CCRYGG 4 cut(s) 45, 156, 732, 961
BstEII GGTNACC 1 cut(s) 727
BstFNI CGCG 2 cut(s) 96, 158
BstH2I RGCGCY 1 cut(s) 680
BstHHI GCGC 3 cut(s) 98, 679, 847
BstKTI GATC 1 cut(s) 205
BstMAI GTCTC 3 cut(s) 166, 298, 709
BstMBI GATC 1 cut(s) 202
BstMWI GCNNNNNNNGC 5 cut(s) 47, 283, 518, 977, 1315
BstNI CCWGG 2 cut(s) 34, 1440
BstNSI RCATGY 1 cut(s) 954
BstPI GGTNACC 1 cut(s) 727
BstSCI CCNGG 4 cut(s) 32, 779, 1222, 1438
BstSFI CTRYAG 1 cut(s) 1383
BstUI CGCG 2 cut(s) 96, 158
BstV1I GCAGC 3 cut(s) 505, 1268, 1382
BstV2I GAAGAC 1 cut(s) 1475
BsuRI GGCC 2 cut(s) 50, 1043
BtgI CCRYGG 4 cut(s) 45, 156, 732, 961
BtgZI GCGATG 1 cut(s) 1290
BtsI GCAGTG 1 cut(s) 1247
BtsIMutI CAGTG 3 cut(s) 490, 756, 1247
BveI ACCTGC 1 cut(s) 65
Cac8I GCNNGC 8 cut(s) 18, 22, 39, 94, 754, 1132, 1254, 1261
CfoI GCGC 3 cut(s) 98, 679, 847
Cfr13I GGNCC 4 cut(s) 49, 630, 910, 1042
Cfr42I CCGCGG 1 cut(s) 159
CseI GACGC 1 cut(s) 1452
Csp6I GTAC 2 cut(s) 1117, 1339
CviAII CATG 9 cut(s) 46, 67, 398, 733, 877, 888, 907, 951, 962
CviQI GTAC 2 cut(s) 1117, 1339
DdeI CTNAG 5 cut(s) 26, 609, 660, 748, 1088
DpnI GATC 1 cut(s) 204
DpnII GATC 1 cut(s) 202
Ecl136II GAGCTC 1 cut(s) 30
Eco130I CCWWGG 6 cut(s) 45, 272, 732, 961, 1351, 1473
Eco24I GRGCYC 2 cut(s) 32, 1089
Eco31I GGTCTC 2 cut(s) 166, 709
Eco32I GATATC 1 cut(s) 642
Eco47I GGWCC 2 cut(s) 630, 910
Eco47III AGCGCT 1 cut(s) 678
Eco53kI GAGCTC 1 cut(s) 30
Eco91I GGTNACC 1 cut(s) 727
EcoICRI GAGCTC 1 cut(s) 30
EcoO65I GGTNACC 1 cut(s) 727
EcoRI GAATTC 2 cut(s) 445, 934
EcoRII CCWGG 2 cut(s) 32, 1438
EcoRV GATATC 1 cut(s) 642
EcoT14I CCWWGG 6 cut(s) 45, 272, 732, 961, 1351, 1473
EcoT22I ATGCAT 3 cut(s) 878, 982, 1544
EcoT38I GRGCYC 2 cut(s) 32, 1089
ErhI CCWWGG 6 cut(s) 45, 272, 732, 961, 1351, 1473
FaeI CATG 9 cut(s) 49, 70, 401, 736, 880, 891, 910, 954, 965
FatI CATG 9 cut(s) 45, 66, 397, 732, 876, 887, 906, 950, 961
FauI CCCGC 2 cut(s) 101, 460
FauNDI CATATG 1 cut(s) 1454
Fnu4HI GCNGC 6 cut(s) 156, 489, 519, 590, 1257, 1371
FriOI GRGCYC 2 cut(s) 32, 1089
Fsp4HI GCNGC 6 cut(s) 156, 489, 519, 590, 1257, 1371
FspBI CTAG 3 cut(s) 492, 1428, 1449
GlaI GCGC 3 cut(s) 97, 678, 846
GluI GCNGC 6 cut(s) 156, 489, 519, 590, 1257, 1371
GsuI CTGGAG 1 cut(s) 16
HaeII RGCGCY 1 cut(s) 680
HaeIII GGCC 2 cut(s) 50, 1043
HapII CCGG 6 cut(s) 171, 719, 780, 1223, 1325, 1483
HgaI GACGC 1 cut(s) 1452
HhaI GCGC 3 cut(s) 98, 679, 847
Hin1II CATG 9 cut(s) 49, 70, 401, 736, 880, 891, 910, 954, 965
Hin6I GCGC 3 cut(s) 96, 677, 845
HinP1I GCGC 3 cut(s) 96, 677, 845
HinfI GANTC 7 cut(s) 454, 478, 1122, 1219, 1435, 1499, 1532
HpaII CCGG 6 cut(s) 171, 719, 780, 1223, 1325, 1483
HphI GGTGA 4 cut(s) 156, 185, 748, 1367
Hpy166II GTNNAC 2 cut(s) 333, 630
Hpy188I TCNGA 2 cut(s) 1091, 1554
Hpy188III TCNNGA 3 cut(s) 1483, 1503, 1536
Hpy8I GTNNAC 2 cut(s) 333, 630
Hpy99I CGWCG 2 cut(s) 271, 509
HpyAV CCTTC 3 cut(s) 346, 1076, 1325
HpyCH4III ACNGT 3 cut(s) 198, 704, 897
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 283, 518, 977, 1315
HpyF3I CTNAG 5 cut(s) 26, 609, 660, 748, 1088
Hsp92II CATG 9 cut(s) 49, 70, 401, 736, 880, 891, 910, 954, 965
HspAI GCGC 3 cut(s) 96, 677, 845
Kpn2I TCCGGA 1 cut(s) 1482
KspI CCGCGG 1 cut(s) 159
Kzo9I GATC 1 cut(s) 202
LmnI GCTCC 1 cut(s) 35
Lsp1109I GCAGC 3 cut(s) 505, 1268, 1382
LweI GCATC 4 cut(s) 798, 863, 967, 1529
MaeI CTAG 3 cut(s) 492, 1428, 1449
MaeIII GTNAC 4 cut(s) 173, 251, 727, 1030
MalI GATC 1 cut(s) 204
MboI GATC 1 cut(s) 202
MboII GAAGA 3 cut(s) 220, 382, 1480
MhlI GDGCHC 2 cut(s) 32, 1089
MluCI AATT 4 cut(s) 445, 577, 934, 1203
MlyI GAGTC 1 cut(s) 1429
Mph1103I ATGCAT 3 cut(s) 878, 982, 1544
MroI TCCGGA 1 cut(s) 1482
MseI TTAA 3 cut(s) 179, 1202, 1490
MslI CAYNNNNRTG 3 cut(s) 462, 966, 1561
MspA1I CMGCKG 1 cut(s) 158
MspI CCGG 6 cut(s) 171, 719, 780, 1223, 1325, 1483
MspR9I CCNGG 4 cut(s) 34, 781, 1224, 1440
Mva1269I GAATGC 2 cut(s) 277, 1110
MvaI CCWGG 2 cut(s) 34, 1440
MvnI CGCG 2 cut(s) 96, 158
MwoI GCNNNNNNNGC 5 cut(s) 47, 283, 518, 977, 1315
NciI CCSGG 2 cut(s) 781, 1224
NcoI CCATGG 3 cut(s) 45, 732, 961
NdeI CATATG 1 cut(s) 1454
NdeII GATC 1 cut(s) 202
NlaIII CATG 9 cut(s) 49, 70, 401, 736, 880, 891, 910, 954, 965
NmeAIII GCCGAG 1 cut(s) 114
NmuCI GTSAC 2 cut(s) 173, 1030
NsiI ATGCAT 3 cut(s) 878, 982, 1544
NspI RCATGY 1 cut(s) 954
PctI GAATGC 2 cut(s) 277, 1110
PfeI GAWTC 6 cut(s) 454, 478, 1122, 1219, 1499, 1532
PflMI CCANNNNNTGG 1 cut(s) 1025
PfoI TCCNGGA 1 cut(s) 1438
PkrI GCNGC 6 cut(s) 157, 490, 520, 591, 1258, 1372
PleI GAGTC 1 cut(s) 1429
PpsI GAGTC 1 cut(s) 1429
PshBI ATTAAT 1 cut(s) 1202
Psp124BI GAGCTC 1 cut(s) 32
Psp6I CCWGG 2 cut(s) 32, 1438
PspEI GGTNACC 1 cut(s) 727
PspGI CCWGG 2 cut(s) 32, 1438
PspPI GGNCC 4 cut(s) 49, 630, 910, 1042
PstI CTGCAG 1 cut(s) 1387
RsaI GTAC 2 cut(s) 1118, 1340
RsaNI GTAC 2 cut(s) 1117, 1339
RseI CAYNNNNRTG 3 cut(s) 462, 966, 1561
SacI GAGCTC 1 cut(s) 32
SacII CCGCGG 1 cut(s) 159
SaqAI TTAA 3 cut(s) 179, 1202, 1490
SatI GCNGC 6 cut(s) 156, 489, 519, 590, 1257, 1371
Sau3AI GATC 1 cut(s) 202
Sau96I GGNCC 4 cut(s) 49, 630, 910, 1042
SchI GAGTC 1 cut(s) 1429
ScrFI CCNGG 4 cut(s) 34, 781, 1224, 1440
SduI GDGCHC 2 cut(s) 32, 1089
SfaNI GCATC 4 cut(s) 798, 863, 967, 1529
SfcI CTRYAG 1 cut(s) 1383
Sfr303I CCGCGG 1 cut(s) 159
SgrBI CCGCGG 1 cut(s) 159
SinI GGWCC 2 cut(s) 630, 910
SmiMI CAYNNNNRTG 3 cut(s) 462, 966, 1561
SmlI CTYRAG 2 cut(s) 77, 680
SmoI CTYRAG 2 cut(s) 77, 680
Sse9I AATT 4 cut(s) 445, 577, 934, 1203
SsiI CCGC 7 cut(s) 94, 156, 158, 467, 488, 589, 633
SspMI CTAG 3 cut(s) 492, 1428, 1449
SstI GAGCTC 1 cut(s) 32
StyD4I CCNGG 4 cut(s) 32, 779, 1222, 1438
StyI CCWWGG 6 cut(s) 45, 272, 732, 961, 1351, 1473
TaaI ACNGT 3 cut(s) 198, 704, 897
TaqI TCGA 6 cut(s) 201, 362, 381, 504, 668, 1125
TaqII GACCGA 1 cut(s) 927
TasI AATT 4 cut(s) 445, 577, 934, 1203
TatI WGTACW 1 cut(s) 1116
TauI GCSGC 3 cut(s) 158, 491, 592
TfiI GAWTC 6 cut(s) 454, 478, 1122, 1219, 1499, 1532
Tru1I TTAA 3 cut(s) 179, 1202, 1490
Tru9I TTAA 3 cut(s) 179, 1202, 1490
TscAI CASTG 3 cut(s) 490, 763, 1254
TseFI GTSAC 2 cut(s) 173, 1030
TseI GCWGC 3 cut(s) 518, 1256, 1370
Tsp45I GTSAC 2 cut(s) 173, 1030
TspGWI ACGGA 2 cut(s) 137, 1480
TspRI CASTG 3 cut(s) 490, 763, 1254
Van91I CCANNNNNTGG 1 cut(s) 1025
VpaK11BI GGWCC 2 cut(s) 630, 910
VspI ATTAAT 1 cut(s) 1202
XapI RAATTY 3 cut(s) 445, 577, 934
XceI RCATGY 1 cut(s) 954
XspI CTAG 3 cut(s) 492, 1428, 1449
Zsp2I ATGCAT 3 cut(s) 878, 982, 1544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.