MD09G1084700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0009703
Physical Location & Seq
Forward (+)
6066743 .. 6072026
5284 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1084700.v1.1.491

Sequence Viewer

Length: 1404 bp
ATGGTAATTGTCCAAGCTTCCAAGCTCAGCCTCCCAAACCCTTCTCTCTCCTCCCCTCCCCAAATAACGTCCCTCCAATTTGAACCCCATTCTCTCTCTCTAGCCCTCATGCACTCTGACTCCACTTTCTCTCTCTACCCCTCTCTCCCCTCCCCTCTTTCCCTCTCCTCCCTCCCTCCTCCCCAAACCCTAATTGCCCCTCCCTCATCATCCTCCACCTTCCTCCTTCTGCAAAACCCTAATTCTAGCCCCAACACCTGGACCCTCTTCATTGCCTCCGGCCCTCACAAAGGTGGGTCCCAGGTCCTGCTCAGGTTCTACATTCTGCAGAACCAGAAGCAATTCGTGAGGGCTCAAGTTGTTTGCACCCAGAAAGGGCTGAAATTTGATGGGAAATTGGGGGTTTTGGTTGATGCCTATCATGGGGTTTCGATTAAGCTTGCCGGGTCCGTCAATTTCTTCGCCATGTACTCAGTTTCGAGCTCACAGATTTCGGTTTTTGCTGTGAAAGCAGTTGGCGATGATGGGGTGGTTGTGAAGTTGATGAGGTGTGCTGTCATTGAGTGTTGCAAGCTTGTTTGGTCGATTAGCATTTCGTTTGGGTTCTTGATCCTAGGAGAAGATAATGGAGTCAGGGTTTTCAACTTGAGGCAGCTTGTTAAAGGGCGGGTTCGAAAGGCCAAGAATTTGAGCTCAAGTTCGAAAAATGAGGGCCGAAATTTGGGCTTGCCTAATGGGGTGATTGGTGACCAAGTGCATAGTGATTTGGGCGATTGTGGAAATAAGCATGGAGGCAGCAAATGTGGTGGCGTTGAGGGGAGTTTGGAGATAACTTGCAGTGGTAACTTGTGTGCGAAGAATGATAGGAATTATGTTTCTGTCAAGCAGAGGTCTGTTAAACTGAACCAAGACTCTTGTGAAGAGGGAGTATGTTTTGTGGAATTCAAGGGAAAGGAGTTTGAAACCTCAAAATCGAAAAGAACGATACCAGCAAAGGCAATTTCTATTGAAGCACTATCCCCCAACAAATTTTTAATCTTGGATTCAGATGGAGGTTTACGCATTTTGCACCTCTCCGGTCCTGTAATTGGATCAGAAATAACTTCTTGCGTGCAGCAGTTGCCTCACATTATGAAAGTGCAAAAGCTGGCTGTTCTTCCTGATATAGATTCACGAACTCAATCTGTTTGGGCATCAGATGGATACAATTCTGTGCAGATGATGTTGGCATCTGACGTGGATATTGCAGAAAATGGGAAAAATGACAGTGAGGAAAAGCTGATTCATGTCTCAGTTCTTCTTTCAGTTTTTGCCAGCGAAAAAATTCAAGATCTTATTCCTATGGCTGCAAATGCTATTCTGATTCTTGGACAAGGAAGCTTGTACACATATGCGATTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

468

Amino Acids

50.19

Weight (kDa)

8.65

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 258
AciI CCGC 1 cut(s) 667
AclWI GGATC 2 cut(s) 604, 1099
AcsI RAATTY 6 cut(s) 383, 685, 718, 941, 1028, 1323
AfaI GTAC 2 cut(s) 470, 1385
AfiI CCNNNNNNNGG 6 cut(s) 258, 290, 375, 423, 721, 1088
AgsI TTSAA 6 cut(s) 83, 643, 946, 962, 1010, 1328
AjiI CACGTC 1 cut(s) 1237
AjnI CCWGG 2 cut(s) 257, 300
AleI CACNNNNGTG 1 cut(s) 291
Alw21I GWGCWC 2 cut(s) 485, 695
Alw26I GTCTC 1 cut(s) 1294
AlwI GGATC 2 cut(s) 604, 1099
AlwNI CAGNNNCTG 1 cut(s) 307
AoxI GGCC 3 cut(s) 280, 678, 712
ApeKI GCWGC 4 cut(s) 652, 795, 1114, 1346
ApoI RAATTY 6 cut(s) 383, 685, 718, 941, 1028, 1323
Asp700I GAANNNNTTC 2 cut(s) 341, 1323
AspA2I CCTAGG 1 cut(s) 613
AspS9I GGNCC 7 cut(s) 261, 281, 297, 304, 447, 712, 1079
AsuC2I CCSGG 1 cut(s) 445
AsuHPI GGTGA 2 cut(s) 751, 758
AsuII TTCGAA 2 cut(s) 673, 701
AvaII GGWCC 5 cut(s) 261, 297, 304, 447, 1079
AvrII CCTAGG 1 cut(s) 613
BanII GRGCYC 3 cut(s) 355, 485, 695
Bbv12I GWGCWC 2 cut(s) 485, 695
BbvI GCAGC 4 cut(s) 664, 807, 1126, 1333
BccI CCATC 4 cut(s) 383, 518, 1043, 1193
BciT130I CCWGG 2 cut(s) 259, 302
BciVI GTATCC 1 cut(s) 1196
BcnI CCSGG 1 cut(s) 445
BcoDI GTCTC 1 cut(s) 1294
BfaI CTAG 3 cut(s) 101, 246, 614
BfmI CTRYAG 1 cut(s) 326
BfuI GTATCC 1 cut(s) 1196
BglII AGATCT 1 cut(s) 1330
BisI GCNGC 4 cut(s) 653, 796, 1115, 1347
BlnI CCTAGG 1 cut(s) 613
BlpI GCTNAGC 1 cut(s) 26
BlsI GCNGC 4 cut(s) 654, 797, 1116, 1348
Bme1390I CCNGG 3 cut(s) 259, 302, 445
Bme18I GGWCC 5 cut(s) 261, 297, 304, 447, 1079
BmgBI CACGTC 1 cut(s) 1237
BmgT120I GGNCC 7 cut(s) 261, 281, 297, 304, 447, 712, 1079
BmiI GGNNCC 4 cut(s) 263, 298, 299, 448
BmrFI CCNGG 3 cut(s) 259, 302, 445
BmsI GCATC 3 cut(s) 403, 1202, 1238
Bpu10I CCTNAGC 1 cut(s) 311
Bpu1102I GCTNAGC 1 cut(s) 26
Bpu14I TTCGAA 2 cut(s) 673, 701
BpuEI CTTGAG 3 cut(s) 339, 667, 679
BpuMI CCSGG 1 cut(s) 445
BsaBI GATNNNNATC 1 cut(s) 417
BsaJI CCNNGG 2 cut(s) 300, 613
BsaWI WCCGGW 1 cut(s) 1076
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 621, 651
Bsc4I CCNNNNNNNGG 6 cut(s) 258, 290, 375, 423, 721, 1088
Bse3DI GCAATG 1 cut(s) 270
Bse8I GATNNNNATC 1 cut(s) 417
BseBI CCWGG 2 cut(s) 259, 302
BseDI CCNNGG 2 cut(s) 300, 613
BseGI GGATG 1 cut(s) 209
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 6 cut(s) 258, 290, 375, 423, 721, 1088
BseMI GCAATG 1 cut(s) 270
BseMII CTCAG 4 cut(s) 40, 325, 486, 1305
BseRI GAGGAG 3 cut(s) 40, 157, 168
BseXI GCAGC 4 cut(s) 664, 807, 1126, 1333
BsgI GTGCAG 2 cut(s) 1133, 1235
BshFI GGCC 3 cut(s) 282, 680, 714
BsiHKAI GWGCWC 2 cut(s) 485, 695
BsiSI CCGG 3 cut(s) 279, 444, 1077
BslFI GGGAC 2 cut(s) 55, 283
BslI CCNNNNNNNGG 6 cut(s) 258, 290, 375, 423, 721, 1088
BsmAI GTCTC 1 cut(s) 1294
BsmFI GGGAC 2 cut(s) 55, 283
BsnI GGCC 3 cut(s) 282, 680, 714
Bsp119I TTCGAA 2 cut(s) 673, 701
Bsp1286I GDGCHC 3 cut(s) 355, 485, 695
Bsp1407I TGTACA 1 cut(s) 1383
Bsp143I GATC 3 cut(s) 609, 1091, 1330
Bsp1720I GCTNAGC 1 cut(s) 26
BspACI CCGC 1 cut(s) 667
BspANI GGCC 3 cut(s) 282, 680, 714
BspCNI CTCAG 4 cut(s) 39, 324, 485, 1304
BspLI GGNNCC 4 cut(s) 263, 298, 299, 448
BspMAI CTGCAG 1 cut(s) 330
BspPI GGATC 2 cut(s) 604, 1099
BspT104I TTCGAA 2 cut(s) 673, 701
BsrDI GCAATG 1 cut(s) 270
BsrGI TGTACA 1 cut(s) 1383
BssECI CCNNGG 2 cut(s) 300, 613
BssMI GATC 3 cut(s) 609, 1091, 1330
BssT1I CCWWGG 1 cut(s) 613
Bst2UI CCWGG 2 cut(s) 259, 302
Bst4CI ACNGT 1 cut(s) 1268
Bst6I CTCTTC 2 cut(s) 272, 915
BstAPI GCANNNNNTGC 1 cut(s) 1120
BstAUI TGTACA 1 cut(s) 1383
BstBI TTCGAA 2 cut(s) 673, 701
BstC8I GCNNGC 6 cut(s) 441, 572, 728, 1112, 1149, 1315
BstDEI CTNAG 4 cut(s) 26, 311, 472, 1291
BstEII GGTNACC 1 cut(s) 746
BstENI CCTNNNNNAGG 1 cut(s) 288
BstF5I GGATG 1 cut(s) 209
BstKTI GATC 3 cut(s) 612, 1094, 1333
BstMAI GTCTC 1 cut(s) 1294
BstMBI GATC 3 cut(s) 609, 1091, 1330
BstMWI GCNNNNNNNGC 3 cut(s) 509, 1120, 1352
BstNI CCWGG 2 cut(s) 259, 302
BstPI GGTNACC 1 cut(s) 746
BstSCI CCNGG 3 cut(s) 257, 300, 443
BstSFI CTRYAG 1 cut(s) 326
BstV1I GCAGC 4 cut(s) 664, 807, 1126, 1333
BstX2I RGATCY 1 cut(s) 1330
BstYI RGATCY 1 cut(s) 1330
BsuI GTATCC 1 cut(s) 1196
BsuRI GGCC 3 cut(s) 282, 680, 714
BtgZI GCGATG 1 cut(s) 534
BtrI CACGTC 1 cut(s) 1237
BtsCI GGATG 1 cut(s) 209
BtsI GCAGTG 1 cut(s) 844
BtsIMutI CAGTG 2 cut(s) 844, 1273
Cac8I GCNNGC 6 cut(s) 441, 572, 728, 1112, 1149, 1315
CaiI CAGNNNCTG 1 cut(s) 307
Cfr13I GGNCC 7 cut(s) 261, 281, 297, 304, 447, 712, 1079
Csp6I GTAC 2 cut(s) 469, 1384
CspCI CAANNNNNGTGG 2 cut(s) 787, 822
CviAII CATG 5 cut(s) 109, 422, 466, 788, 1286
CviQI GTAC 2 cut(s) 469, 1384
DdeI CTNAG 4 cut(s) 26, 311, 472, 1291
DpnI GATC 3 cut(s) 611, 1093, 1332
DpnII GATC 3 cut(s) 609, 1091, 1330
Eam1104I CTCTTC 2 cut(s) 272, 915
EarI CTCTTC 2 cut(s) 272, 915
Ecl136II GAGCTC 2 cut(s) 483, 693
Eco130I CCWWGG 1 cut(s) 613
Eco24I GRGCYC 3 cut(s) 355, 485, 695
Eco47I GGWCC 5 cut(s) 261, 297, 304, 447, 1079
Eco53kI GAGCTC 2 cut(s) 483, 693
Eco91I GGTNACC 1 cut(s) 746
EcoICRI GAGCTC 2 cut(s) 483, 693
EcoNI CCTNNNNNAGG 1 cut(s) 288
EcoO109I RGGNCCY 2 cut(s) 297, 304
EcoO65I GGTNACC 1 cut(s) 746
EcoRI GAATTC 1 cut(s) 941
EcoRII CCWGG 2 cut(s) 257, 300
EcoT14I CCWWGG 1 cut(s) 613
EcoT38I GRGCYC 3 cut(s) 355, 485, 695
ErhI CCWWGG 1 cut(s) 613
FaeI CATG 5 cut(s) 112, 425, 469, 791, 1289
FaqI GGGAC 2 cut(s) 55, 283
FatI CATG 5 cut(s) 108, 421, 465, 787, 1285
FauI CCCGC 1 cut(s) 660
FauNDI CATATG 1 cut(s) 1390
Fnu4HI GCNGC 4 cut(s) 653, 796, 1115, 1347
FokI GGATG 1 cut(s) 196
FriOI GRGCYC 3 cut(s) 355, 485, 695
Fsp4HI GCNGC 4 cut(s) 653, 796, 1115, 1347
FspBI CTAG 3 cut(s) 101, 246, 614
GluI GCNGC 4 cut(s) 653, 796, 1115, 1347
HaeIII GGCC 3 cut(s) 282, 680, 714
HapII CCGG 3 cut(s) 279, 444, 1077
Hin1II CATG 5 cut(s) 112, 425, 469, 791, 1289
HindIII AAGCTT 4 cut(s) 15, 437, 572, 1378
HinfI GANTC 7 cut(s) 119, 630, 911, 1043, 1169, 1282, 1363
HpaII CCGG 3 cut(s) 279, 444, 1077
HphI GGTGA 2 cut(s) 751, 758
Hpy166II GTNNAC 2 cut(s) 1058, 1386
Hpy188I TCNGA 6 cut(s) 118, 1048, 1096, 1198, 1234, 1362
Hpy188III TCNNGA 6 cut(s) 346, 607, 1160, 1173, 1328, 1401
Hpy8I GTNNAC 2 cut(s) 1058, 1386
HpyAV CCTTC 3 cut(s) 51, 229, 236
HpyCH4III ACNGT 1 cut(s) 1268
HpyCH4IV ACGT 2 cut(s) 68, 1236
HpyF10VI GCNNNNNNNGC 3 cut(s) 509, 1120, 1352
HpyF3I CTNAG 4 cut(s) 26, 311, 472, 1291
HpySE526I ACGT 2 cut(s) 68, 1236
Hsp92II CATG 5 cut(s) 112, 425, 469, 791, 1289
KflI GGGWCCC 1 cut(s) 297
Kzo9I GATC 3 cut(s) 609, 1091, 1330
Lsp1109I GCAGC 4 cut(s) 664, 807, 1126, 1333
LweI GCATC 3 cut(s) 403, 1202, 1238
MaeI CTAG 3 cut(s) 101, 246, 614
MaeII ACGT 2 cut(s) 68, 1236
MaeIII GTNAC 2 cut(s) 746, 842
MalI GATC 3 cut(s) 611, 1093, 1332
MboI GATC 3 cut(s) 609, 1091, 1330
MboII GAAGA 7 cut(s) 259, 451, 632, 868, 932, 1148, 1289
MflI RGATCY 1 cut(s) 1330
MhlI GDGCHC 3 cut(s) 355, 485, 695
MlyI GAGTC 3 cut(s) 113, 639, 905
MroXI GAANNNNTTC 2 cut(s) 341, 1323
MseI TTAA 4 cut(s) 435, 660, 897, 1034
MslI CAYNNNNRTG 1 cut(s) 291
MspI CCGG 3 cut(s) 279, 444, 1077
MspR9I CCNGG 3 cut(s) 259, 302, 445
MvaI CCWGG 2 cut(s) 259, 302
MwoI GCNNNNNNNGC 3 cut(s) 509, 1120, 1352
NciI CCSGG 1 cut(s) 445
NdeI CATATG 1 cut(s) 1390
NdeII GATC 3 cut(s) 609, 1091, 1330
NlaIII CATG 5 cut(s) 112, 425, 469, 791, 1289
NlaIV GGNNCC 4 cut(s) 263, 298, 299, 448
NmuCI GTSAC 1 cut(s) 746
NspV TTCGAA 2 cut(s) 673, 701
OliI CACNNNNGTG 1 cut(s) 291
PcsI WCGNNNNNNNCGW 1 cut(s) 980
PdmI GAANNNNTTC 2 cut(s) 341, 1323
PfeI GAWTC 4 cut(s) 1043, 1169, 1282, 1363
PflMI CCANNNNNTGG 1 cut(s) 258
PkrI GCNGC 4 cut(s) 654, 797, 1116, 1348
PleI GAGTC 3 cut(s) 113, 638, 905
PpsI GAGTC 3 cut(s) 113, 638, 905
PpuMI RGGWCCY 2 cut(s) 297, 304
Psp124BI GAGCTC 2 cut(s) 485, 695
Psp5II RGGWCCY 2 cut(s) 297, 304
Psp6I CCWGG 2 cut(s) 257, 300
PspEI GGTNACC 1 cut(s) 746
PspGI CCWGG 2 cut(s) 257, 300
PspN4I GGNNCC 4 cut(s) 263, 298, 299, 448
PspPI GGNCC 7 cut(s) 261, 281, 297, 304, 447, 712, 1079
PspPPI RGGWCCY 2 cut(s) 297, 304
PstI CTGCAG 1 cut(s) 330
PstNI CAGNNNCTG 1 cut(s) 307
PsuI RGATCY 1 cut(s) 1330
RsaI GTAC 2 cut(s) 470, 1385
RsaNI GTAC 2 cut(s) 469, 1384
RseI CAYNNNNRTG 1 cut(s) 291
SacI GAGCTC 2 cut(s) 485, 695
SaqAI TTAA 4 cut(s) 435, 660, 897, 1034
SatI GCNGC 4 cut(s) 653, 796, 1115, 1347
Sau3AI GATC 3 cut(s) 609, 1091, 1330
Sau96I GGNCC 7 cut(s) 261, 281, 297, 304, 447, 712, 1079
SchI GAGTC 3 cut(s) 113, 639, 905
ScrFI CCNGG 3 cut(s) 259, 302, 445
SduI GDGCHC 3 cut(s) 355, 485, 695
SfaNI GCATC 3 cut(s) 403, 1202, 1238
SfcI CTRYAG 1 cut(s) 326
SfuI TTCGAA 2 cut(s) 673, 701
SinI GGWCC 5 cut(s) 261, 297, 304, 447, 1079
SmiMI CAYNNNNRTG 1 cut(s) 291
SmlI CTYRAG 3 cut(s) 354, 646, 694
SmoI CTYRAG 3 cut(s) 354, 646, 694
SsiI CCGC 1 cut(s) 667
SspMI CTAG 3 cut(s) 101, 246, 614
SstI GAGCTC 2 cut(s) 485, 695
StyD4I CCNGG 3 cut(s) 257, 300, 443
StyI CCWWGG 1 cut(s) 613
TaaI ACNGT 1 cut(s) 1268
TaiI ACGT 2 cut(s) 71, 1239
TaqI TCGA 6 cut(s) 431, 479, 584, 673, 701, 974
TatI WGTACW 2 cut(s) 468, 1383
TfiI GAWTC 4 cut(s) 1043, 1169, 1282, 1363
Tru1I TTAA 4 cut(s) 435, 660, 897, 1034
Tru9I TTAA 4 cut(s) 435, 660, 897, 1034
TscAI CASTG 2 cut(s) 844, 1273
TseFI GTSAC 1 cut(s) 746
TseI GCWGC 4 cut(s) 652, 795, 1114, 1346
Tsp45I GTSAC 1 cut(s) 746
TspDTI ATGAA 3 cut(s) 259, 1148, 1274
TspGWI ACGGA 1 cut(s) 439
TspRI CASTG 2 cut(s) 844, 1273
Van91I CCANNNNNTGG 1 cut(s) 258
VpaK11BI GGWCC 5 cut(s) 261, 297, 304, 447, 1079
XagI CCTNNNNNAGG 1 cut(s) 288
XapI RAATTY 6 cut(s) 383, 685, 718, 941, 1028, 1323
XmaJI CCTAGG 1 cut(s) 613
XmnI GAANNNNTTC 2 cut(s) 341, 1323
XspI CTAG 3 cut(s) 101, 246, 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.