RLG00000021377

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009703
Physical Location & Seq
Reverse (-)
74686327 .. 74690498
4172 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021377

Sequence Viewer

Length: 1389 bp
ATGGTTGTTGTCCAAGCCTCCAAGCTCAACCTCCCCTACCCTTCCCTCTCTTCCCCTCACATCAGCTCTCTCCTCTTTGAACCCAACTCTCTCTCTCTAGCCCTCATGCACTCTGACTCCTCTTTCTCTCTCTACCCTTCTCTCTCCTCCCCTCTCTCTCTCTCCTCCCTCCCCCCTCCCCAAACCCTAATCCACCCCCCTTCCTCCTCCTCCACTTTCCTCCTCCTTCAAAACCCTAACCCTAACCCTAACCCCAATCCCAATCCCAATCCCAGAGTGCTTTTCATTGTCTCAGGGCCTTATAAAGGTGGGTCCCAGGTACTGCTCAGGTTCTATATTCTGCACAAGCAGAGCCAATTCGTGAGGGCCCAAGTCGTCTGCACTCAGAAAGGTCTCAACTTTGATCACAAATTGGGGGTTTTGGTGGATTCTAATCATGGGGTTTCGATTAGGCTTTCTGGGTCTGTCAATTTCTTTGCAATGCATTCGGTTTCCGGCTCCAAGATTTCGGTTTTCGCCGTCAAAGATGTTGGTGGTGATGGGGTGGCTGTGAAATTGATGAGGTGTGCTGTCATTGATTGCTGCAAGCTTGTGTGGTCCATTAGCGTTTCGTTCGGGTTCTTGATTCTGGGGGAAGATGATGGAGTGAGGGTTTTCAATTTGAGGCAGCTTGTGAAAGGGCGGGTTCCAAAGGCTAAGAGTTCGAATTCGTGTTTGAAATTGGGTTTGTTAAATGGGGTGATTGGTGACAATGTGCATAGTGAAGCTGGGGATTGTGGAAATAAACATGGAAGTGGAAAGTGTGAAGGGACTTGCGATGGTTACTTGTGCCGGAAGAATGATAGGAATTGTGTCTCTGTCAAGCAGAGATCTGTTAAACTGAAACAAGACTCATGTGAAGACGGAGTGTGTTTTGCGGCGTTCAAGGGCAAGGAGTTAGAAACATCTAAATCAAGAAGAAGGATACCAGTGAAGGCAATTTCCATTGACGCACTGTCCCCTAACAAACTTTTAATCCTGGACTCAGATGGAGGTTTACAACTTTTGCACCTTTCCATCCCTGTAATTGGATCAGATATAACTACTTTCATGCAGCAGTTGCCTAACACTATGAAAGTGCAAAAGGTGGCTGTTCTTCCTGAAATAGCTGCAAGAACACAATCTGTTTGGGTGTCAGATGGCTACAATTCTGTGCAGATGATGGTGGCATCTGACATGGATAGTGCAGTTACCCAAAATGATTTAAATGAGAGCAAGGAAAAGCCGATGCATATCACAGTTGGTCTCACAATTTTTACTGGGGAAAAGATTCAAGACCTTACTCCTTTGGCCGGAAATGCTATTTTGATTCTTGGACAAGGAAACTTGTACACGTATGCTACCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

463

Amino Acids

49.78

Weight (kDa)

8.97

Isoelectric Point (pI)

44.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
AciI CCGC 2 cut(s) 682, 917
AclWI GGATC 1 cut(s) 1078
AcoI YGGCCR 1 cut(s) 1329
AcsI RAATTY 1 cut(s) 706
AfaI GTAC 2 cut(s) 321, 1370
AfiI CCNNNNNNNGG 3 cut(s) 305, 1067, 1331
AflIII ACRYGT 1 cut(s) 1371
AgsI TTSAA 6 cut(s) 80, 230, 658, 718, 925, 1313
AhdI GACNNNNNGTC 1 cut(s) 994
AjnI CCWGG 2 cut(s) 315, 1017
AluBI AGCT 6 cut(s) 25, 66, 589, 670, 767, 1148
AluI AGCT 6 cut(s) 25, 66, 589, 670, 767, 1148
Alw26I GTCTC 4 cut(s) 295, 398, 859, 1289
AlwI GGATC 1 cut(s) 1078
AlwNI CAGNNNCTG 1 cut(s) 322
AoxI GGCC 3 cut(s) 296, 366, 1329
ApaI GGGCCC 1 cut(s) 370
ApeKI GCWGC 4 cut(s) 582, 667, 1093, 1148
ApoI RAATTY 1 cut(s) 706
Asp700I GAANNNNTTC 1 cut(s) 1308
AspS9I GGNCC 5 cut(s) 296, 312, 366, 367, 597
AsuHPI GGTGA 3 cut(s) 548, 751, 758
AsuII TTCGAA 1 cut(s) 704
AvaII GGWCC 2 cut(s) 312, 597
BaeGI GKGCMC 1 cut(s) 370
BanII GRGCYC 1 cut(s) 370
BbsI GAAGAC 1 cut(s) 906
BbvI GCAGC 4 cut(s) 569, 679, 1105, 1135
BccI CCATC 7 cut(s) 533, 635, 812, 1022, 1064, 1172, 1195
BceAI ACGGC 1 cut(s) 503
BcgI CGANNNNNNTGC 2 cut(s) 468, 502
BciT130I CCWGG 2 cut(s) 317, 1019
BciVI GTATCC 1 cut(s) 957
BclI TGATCA 1 cut(s) 403
BcoDI GTCTC 4 cut(s) 295, 398, 859, 1289
BfaI CTAG 1 cut(s) 98
BfuI GTATCC 1 cut(s) 957
BglII AGATCT 1 cut(s) 869
BisI GCNGC 5 cut(s) 583, 668, 918, 1094, 1149
BlsI GCNGC 5 cut(s) 584, 669, 919, 1095, 1150
Bme1390I CCNGG 2 cut(s) 317, 1019
Bme18I GGWCC 2 cut(s) 312, 597
BmeRI GACNNNNNGTC 1 cut(s) 994
BmgT120I GGNCC 5 cut(s) 296, 312, 366, 367, 597
BmiI GGNNCC 5 cut(s) 313, 314, 368, 499, 687
BmrFI CCNGG 2 cut(s) 317, 1019
BmrI ACTGGG 1 cut(s) 1308
BmsI GCATC 2 cut(s) 1217, 1257
BmuI ACTGGG 1 cut(s) 1308
BpiI GAAGAC 1 cut(s) 906
Bpu10I CCTNAGC 1 cut(s) 326
Bpu14I TTCGAA 1 cut(s) 704
BsaAI YACGTR 1 cut(s) 1374
BsaBI GATNNNNATC 2 cut(s) 432, 1271
BsaI GGTCTC 2 cut(s) 398, 1289
BsaJI CCNNGG 1 cut(s) 315
BsaXI ACNNNNNCTCC 4 cut(s) 101, 131, 636, 666
Bsc4I CCNNNNNNNGG 3 cut(s) 305, 1067, 1331
Bse1I ACTGG 2 cut(s) 968, 1303
Bse3DI GCAATG 1 cut(s) 486
Bse8I GATNNNNATC 2 cut(s) 432, 1271
BseBI CCWGG 2 cut(s) 317, 1019
BseDI CCNNGG 1 cut(s) 315
BseGI GGATG 1 cut(s) 1056
BseJI GATNNNNATC 2 cut(s) 432, 1271
BseLI CCNNNNNNNGG 3 cut(s) 305, 1067, 1331
BseMI GCAATG 1 cut(s) 486
BseMII CTCAG 4 cut(s) 306, 340, 398, 1038
BseNI ACTGG 2 cut(s) 968, 1303
BseRI GAGGAG 7 cut(s) 62, 109, 136, 154, 196, 199, 212
BseSI GKGCMC 1 cut(s) 370
BseXI GCAGC 4 cut(s) 569, 679, 1105, 1135
BseYI CCCAGC 1 cut(s) 767
BsgI GTGCAG 4 cut(s) 326, 364, 1214, 1245
BshFI GGCC 3 cut(s) 298, 368, 1331
BsiSI CCGG 3 cut(s) 495, 832, 1332
BslFI GGGAC 3 cut(s) 298, 823, 982
BslI CCNNNNNNNGG 3 cut(s) 305, 1067, 1331
BsmAI GTCTC 4 cut(s) 295, 398, 859, 1289
BsmFI GGGAC 3 cut(s) 298, 823, 982
BsmI GAATGC 1 cut(s) 484
BsnI GGCC 3 cut(s) 298, 368, 1331
Bso31I GGTCTC 2 cut(s) 398, 1289
Bsp119I TTCGAA 1 cut(s) 704
Bsp120I GGGCCC 1 cut(s) 366
Bsp1286I GDGCHC 1 cut(s) 370
Bsp1407I TGTACA 1 cut(s) 1368
Bsp143I GATC 3 cut(s) 403, 869, 1070
BspACI CCGC 2 cut(s) 682, 917
BspANI GGCC 3 cut(s) 298, 368, 1331
BspCNI CTCAG 4 cut(s) 305, 339, 397, 1037
BspLI GGNNCC 5 cut(s) 313, 314, 368, 499, 687
BspPI GGATC 1 cut(s) 1078
BspT104I TTCGAA 1 cut(s) 704
BspTNI GGTCTC 2 cut(s) 398, 1289
BsrDI GCAATG 1 cut(s) 486
BsrGI TGTACA 1 cut(s) 1368
BsrI ACTGG 2 cut(s) 968, 1303
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 3 cut(s) 403, 869, 1070
Bst2UI CCWGG 2 cut(s) 317, 1019
Bst4CI ACNGT 2 cut(s) 996, 1279
Bst6I CTCTTC 1 cut(s) 55
BstAPI GCANNNNNTGC 1 cut(s) 1099
BstAUI TGTACA 1 cut(s) 1368
BstBAI YACGTR 1 cut(s) 1374
BstBI TTCGAA 1 cut(s) 704
BstC8I GCNNGC 1 cut(s) 587
BstDEI CTNAG 5 cut(s) 292, 326, 384, 696, 1024
BstENI CCTNNNNNAGG 1 cut(s) 303
BstF5I GGATG 1 cut(s) 1056
BstKTI GATC 3 cut(s) 406, 872, 1073
BstMAI GTCTC 4 cut(s) 295, 398, 859, 1289
BstMBI GATC 3 cut(s) 403, 869, 1070
BstMWI GCNNNNNNNGC 2 cut(s) 1099, 1337
BstNI CCWGG 2 cut(s) 317, 1019
BstSCI CCNGG 2 cut(s) 315, 1017
BstSLI GKGCMC 1 cut(s) 370
BstV1I GCAGC 4 cut(s) 569, 679, 1105, 1135
BstV2I GAAGAC 1 cut(s) 906
BstX2I RGATCY 1 cut(s) 869
BstYI RGATCY 1 cut(s) 869
BsuI GTATCC 1 cut(s) 957
BsuRI GGCC 3 cut(s) 298, 368, 1331
BtgZI GCGATG 1 cut(s) 831
BtsCI GGATG 1 cut(s) 1056
BtsIMutI CAGTG 2 cut(s) 975, 992
Cac8I GCNNGC 1 cut(s) 587
CaiI CAGNNNCTG 1 cut(s) 322
Cfr13I GGNCC 5 cut(s) 296, 312, 366, 367, 597
CseI GACGC 1 cut(s) 998
Csp6I GTAC 2 cut(s) 320, 1369
CviAII CATG 6 cut(s) 106, 437, 788, 894, 1090, 1216
CviQI GTAC 2 cut(s) 320, 1369
DdeI CTNAG 5 cut(s) 292, 326, 384, 696, 1024
DpnI GATC 3 cut(s) 405, 871, 1072
DpnII GATC 3 cut(s) 403, 869, 1070
DraI TTTAAA 1 cut(s) 1245
DriI GACNNNNNGTC 1 cut(s) 994
EaeI YGGCCR 1 cut(s) 1329
Eam1104I CTCTTC 1 cut(s) 55
Eam1105I GACNNNNNGTC 1 cut(s) 994
EarI CTCTTC 1 cut(s) 55
Eco24I GRGCYC 1 cut(s) 370
Eco31I GGTCTC 2 cut(s) 398, 1289
Eco47I GGWCC 2 cut(s) 312, 597
EcoNI CCTNNNNNAGG 1 cut(s) 303
EcoO109I RGGNCCY 3 cut(s) 296, 312, 366
EcoRI GAATTC 1 cut(s) 706
EcoRII CCWGG 2 cut(s) 315, 1017
EcoT22I ATGCAT 2 cut(s) 486, 1272
EcoT38I GRGCYC 1 cut(s) 370
FaeI CATG 6 cut(s) 109, 440, 791, 897, 1093, 1219
FaqI GGGAC 3 cut(s) 298, 823, 982
FatI CATG 6 cut(s) 105, 436, 787, 893, 1089, 1215
FauI CCCGC 1 cut(s) 675
FbaI TGATCA 1 cut(s) 403
Fnu4HI GCNGC 5 cut(s) 583, 668, 918, 1094, 1149
FokI GGATG 1 cut(s) 1043
FriOI GRGCYC 1 cut(s) 370
Fsp4HI GCNGC 5 cut(s) 583, 668, 918, 1094, 1149
FspBI CTAG 1 cut(s) 98
GluI GCNGC 5 cut(s) 583, 668, 918, 1094, 1149
GsaI CCCAGC 1 cut(s) 771
HaeIII GGCC 3 cut(s) 298, 368, 1331
HapII CCGG 3 cut(s) 495, 832, 1332
HgaI GACGC 1 cut(s) 998
Hin1II CATG 6 cut(s) 109, 440, 791, 897, 1093, 1219
HindIII AAGCTT 1 cut(s) 587
HinfI GANTC 7 cut(s) 116, 428, 625, 890, 1022, 1309, 1348
HpaII CCGG 3 cut(s) 495, 832, 1332
HphI GGTGA 3 cut(s) 548, 751, 758
Hpy166II GTNNAC 2 cut(s) 1037, 1371
Hpy188I TCNGA 6 cut(s) 115, 387, 1027, 1075, 1177, 1213
Hpy188III TCNNGA 6 cut(s) 361, 622, 954, 1139, 1313, 1386
Hpy8I GTNNAC 2 cut(s) 1037, 1371
HpyAV CCTTC 7 cut(s) 51, 147, 210, 236, 800, 954, 967
HpyCH4III ACNGT 2 cut(s) 996, 1279
HpyCH4IV ACGT 1 cut(s) 1373
HpyF10VI GCNNNNNNNGC 2 cut(s) 1099, 1337
HpyF3I CTNAG 5 cut(s) 292, 326, 384, 696, 1024
HpySE526I ACGT 1 cut(s) 1373
Hsp92II CATG 6 cut(s) 109, 440, 791, 897, 1093, 1219
KflI GGGWCCC 1 cut(s) 312
Ksp22I TGATCA 1 cut(s) 403
Kzo9I GATC 3 cut(s) 403, 869, 1070
LmnI GCTCC 1 cut(s) 503
Lsp1109I GCAGC 4 cut(s) 569, 679, 1105, 1135
LweI GCATC 2 cut(s) 1217, 1257
MaeI CTAG 1 cut(s) 98
MaeII ACGT 1 cut(s) 1373
MaeIII GTNAC 3 cut(s) 746, 821, 1228
MalI GATC 3 cut(s) 405, 871, 1072
MboI GATC 3 cut(s) 403, 869, 1070
MboII GAAGA 6 cut(s) 42, 647, 847, 911, 969, 1127
MflI RGATCY 1 cut(s) 869
MhlI GDGCHC 1 cut(s) 370
MlyI GAGTC 3 cut(s) 110, 884, 1016
Mph1103I ATGCAT 2 cut(s) 486, 1272
MroXI GAANNNNTTC 1 cut(s) 1308
MseI TTAA 4 cut(s) 731, 876, 1013, 1244
MslI CAYNNNNRTG 1 cut(s) 792
MspI CCGG 3 cut(s) 495, 832, 1332
MspR9I CCNGG 2 cut(s) 317, 1019
Mva1269I GAATGC 1 cut(s) 484
MvaI CCWGG 2 cut(s) 317, 1019
MwoI GCNNNNNNNGC 2 cut(s) 1099, 1337
NdeII GATC 3 cut(s) 403, 869, 1070
NlaIII CATG 6 cut(s) 109, 440, 791, 897, 1093, 1219
NlaIV GGNNCC 5 cut(s) 313, 314, 368, 499, 687
NmuCI GTSAC 1 cut(s) 746
NsiI ATGCAT 2 cut(s) 486, 1272
NspV TTCGAA 1 cut(s) 704
PctI GAATGC 1 cut(s) 484
PdmI GAANNNNTTC 1 cut(s) 1308
PfeI GAWTC 4 cut(s) 428, 625, 1309, 1348
PfoI TCCNGGA 1 cut(s) 1017
PkrI GCNGC 5 cut(s) 584, 669, 919, 1095, 1150
PleI GAGTC 3 cut(s) 110, 884, 1016
PpsI GAGTC 3 cut(s) 110, 884, 1016
Ppu21I YACGTR 1 cut(s) 1374
PpuMI RGGWCCY 1 cut(s) 312
PsiI TTATAA 1 cut(s) 303
Psp5II RGGWCCY 1 cut(s) 312
Psp6I CCWGG 2 cut(s) 315, 1017
PspFI CCCAGC 1 cut(s) 767
PspGI CCWGG 2 cut(s) 315, 1017
PspN4I GGNNCC 5 cut(s) 313, 314, 368, 499, 687
PspOMI GGGCCC 1 cut(s) 366
PspPI GGNCC 5 cut(s) 296, 312, 366, 367, 597
PspPPI RGGWCCY 1 cut(s) 312
PstNI CAGNNNCTG 1 cut(s) 322
PsuI RGATCY 1 cut(s) 869
RsaI GTAC 2 cut(s) 321, 1370
RsaNI GTAC 2 cut(s) 320, 1369
RseI CAYNNNNRTG 1 cut(s) 792
SaqAI TTAA 4 cut(s) 731, 876, 1013, 1244
SatI GCNGC 5 cut(s) 583, 668, 918, 1094, 1149
Sau3AI GATC 3 cut(s) 403, 869, 1070
Sau96I GGNCC 5 cut(s) 296, 312, 366, 367, 597
SchI GAGTC 3 cut(s) 110, 884, 1016
ScrFI CCNGG 2 cut(s) 317, 1019
SduI GDGCHC 1 cut(s) 370
SfaNI GCATC 2 cut(s) 1217, 1257
SfuI TTCGAA 1 cut(s) 704
SinI GGWCC 2 cut(s) 312, 597
SmiI ATTTAAAT 1 cut(s) 1245
SmiMI CAYNNNNRTG 1 cut(s) 792
SsiI CCGC 2 cut(s) 682, 917
SspMI CTAG 1 cut(s) 98
StyD4I CCNGG 2 cut(s) 315, 1017
SwaI ATTTAAAT 1 cut(s) 1245
TaaI ACNGT 2 cut(s) 996, 1279
TaiI ACGT 1 cut(s) 1376
TaqI TCGA 2 cut(s) 446, 704
TatI WGTACW 1 cut(s) 1368
TauI GCSGC 1 cut(s) 920
TfiI GAWTC 4 cut(s) 428, 625, 1309, 1348
Tru1I TTAA 4 cut(s) 731, 876, 1013, 1244
Tru9I TTAA 4 cut(s) 731, 876, 1013, 1244
TscAI CASTG 2 cut(s) 975, 999
TseFI GTSAC 1 cut(s) 746
TseI GCWGC 4 cut(s) 582, 667, 1093, 1148
Tsp45I GTSAC 1 cut(s) 746
TspDTI ATGAA 3 cut(s) 274, 1078, 1127
TspGWI ACGGA 1 cut(s) 918
TspRI CASTG 2 cut(s) 975, 999
VpaK11BI GGWCC 2 cut(s) 312, 597
XagI CCTNNNNNAGG 1 cut(s) 303
XapI RAATTY 1 cut(s) 706
XmnI GAANNNNTTC 1 cut(s) 1308
XspI CTAG 1 cut(s) 98
Zsp2I ATGCAT 2 cut(s) 486, 1272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.