Rroxscaffold_2G00087510

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009703
Physical Location & Seq
Forward (+)
9538044 .. 9545924
7881 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00087510.1

Sequence Viewer

Length: 1464 bp
ATGGATCGCCTTCACGTTGTGTCGGTTTATGTATTATCAGAAGAAAAAAATATTGCTACAATAATGCGAATTTCAAGGCAATATAATCAGCTTTGCCAATGTGGTTTTATAGTGCTTTTCATTGTCTCAGGGCCTTATAAAGGTGGGTCCCAGGTCCTTCTCAGGTTCTATATTCTGCACAAGCAGACCCAATTTGTGAGGGCCCAAGTGGTTTGCTCTCAGAAAGATCTCAACTTTGATCACAAATTGGGGGTTTTGGTGGATTCTAATCATGGGGTTTCGATTAGGCTTTCTGGGTCTGTCAATTTCTTTGCAATGTATTCGGTTTCCGGCTCCAAGATTTCGGTTTTCGCTGTCAAAGATGTCGGTGGTGATGGGGTGGCTGTGAAATTGATGAGGTGTGCTGTCATTGACTGCTGCAAGCTTGTGTGGTCTATTAGCGTTTCGTTCGGGTTCTTGATTCTGGGGGAAGATGATGGAGTGAGGGTTTTCAATTTGAGGCAGCTTGTGAAAGGGCGGGTTCGAAAGGCTAAGAGTTCGAATTCGAGTTTGAAATTGGGTTTGTTAAATGGGGTGATTGGTGACAATGTGCATAGTGAAGCTGGGGGTTGTGGAAATAAGCATGGAAGTGGAAAGTGCGAAGGGACTTGCGATGATTACTTGTGCCGGAAGAATGATAGGAATTGTGTCTCTGTCAAGCGGAGATCTGTTAAACTGAAACAAGACTCATGTGAAGACGGAGTGTGTTTTGCGGCATTCAAGGGCAAGGAGTTAGAAACCTCAAAATCAAGAAGAAGGATACCAGTGAAGGCAATTTCCATTGACGCACTGTCCCCTAACAAACTTTTAATCCTGGACTCAGATGGAGGTTTACAGCTTTTGCACCTTTCCATCCCTGTAATTGGATCAGATATAACTACTTGCATGCAGCAGTTGCCTAACACTATGAAAGTGCAAAAGGTGGCTGTTCTTCCTGAAATAGCTGCAAGAACACAATCTGTTTGGGTGTCAGATGGCTACAATTCTGTGCAGATGATGGTGGCATCTGACATGGATAGTGCAGTTAGCCAAAATGATTTAAATGAGAGCAAGGAAAAGCCGATGCATATCACAGTTGGTCTCACAATTTTTACTGGGGAAAAGATTCAAGACCTTACTCCTTTGGCCGGAAATGCTATTTTGATTCTTGGACAAGGTAACATGAGGCAATATGGAACCGGGACCAATTTGTTGCCTGCCATTGCTTTCCAAATACTGAAGGTCGAGTCTTTCAGCAATCAAAGCACTTCTGTTGTTCTACCTGACTCCACGAGCAAAAACTTTGTTTGCTGTTTTGGTTGCTCAGAGTTGCCTTGTTTGGAGCGTCTCTATTCCAAGGTGGTCAACTGGAGAGCTGAACGTGCATTTGACCAACTGGAGAGCGTTTCTGATGACTCGCCAACTTATGAAACTTTCAGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

487

Amino Acids

53.31

Weight (kDa)

8.7

Isoelectric Point (pI)

35.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 138
AciI CCGC 3 cut(s) 517, 700, 752
AclWI GGATC 2 cut(s) 12, 913
AcoI YGGCCR 1 cut(s) 1164
AcsI RAATTY 2 cut(s) 69, 541
AcuI CTGAAG 1 cut(s) 1277
AdeI CACNNNGTG 1 cut(s) 19
AfiI CCNNNNNNNGG 3 cut(s) 140, 902, 1166
AgsI TTSAA 5 cut(s) 75, 493, 553, 760, 1148
AhdI GACNNNNNGTC 1 cut(s) 829
AjnI CCWGG 2 cut(s) 150, 852
AluBI AGCT 7 cut(s) 91, 424, 505, 602, 877, 983, 1394
AluI AGCT 7 cut(s) 91, 424, 505, 602, 877, 983, 1394
Alw26I GTCTC 4 cut(s) 130, 694, 1124, 1370
AlwI GGATC 2 cut(s) 12, 913
AoxI GGCC 3 cut(s) 131, 201, 1164
ApaI GGGCCC 1 cut(s) 205
ApeKI GCWGC 4 cut(s) 417, 502, 928, 983
ApoI RAATTY 2 cut(s) 69, 541
Asp700I GAANNNNTTC 1 cut(s) 1143
AspS9I GGNCC 6 cut(s) 131, 147, 154, 201, 202, 1221
AsuC2I CCSGG 1 cut(s) 1219
AsuHPI GGTGA 3 cut(s) 383, 586, 593
AsuII TTCGAA 2 cut(s) 523, 539
AvaII GGWCC 3 cut(s) 147, 154, 1221
BaeGI GKGCMC 1 cut(s) 205
BanII GRGCYC 1 cut(s) 205
BauI CACGAG 1 cut(s) 1309
BbsI GAAGAC 1 cut(s) 741
BbvI GCAGC 4 cut(s) 404, 514, 940, 970
BccI CCATC 6 cut(s) 368, 470, 857, 899, 1007, 1030
BcgI CGANNNNNNTGC 2 cut(s) 303, 337
BciT130I CCWGG 2 cut(s) 152, 854
BciVI GTATCC 1 cut(s) 792
BclI TGATCA 1 cut(s) 238
BcnI CCSGG 1 cut(s) 1219
BcoDI GTCTC 4 cut(s) 130, 694, 1124, 1370
BfuI GTATCC 1 cut(s) 792
BglII AGATCT 2 cut(s) 226, 704
BisI GCNGC 5 cut(s) 418, 503, 753, 929, 984
BlsI GCNGC 5 cut(s) 419, 504, 754, 930, 985
Bme1390I CCNGG 3 cut(s) 152, 854, 1219
Bme18I GGWCC 3 cut(s) 147, 154, 1221
BmeRI GACNNNNNGTC 1 cut(s) 829
BmgT120I GGNCC 6 cut(s) 131, 147, 154, 201, 202, 1221
BmiI GGNNCC 6 cut(s) 148, 149, 203, 334, 1216, 1222
BmrFI CCNGG 3 cut(s) 152, 854, 1219
BmrI ACTGGG 1 cut(s) 1143
BmsI GCATC 2 cut(s) 1052, 1092
BmuI ACTGGG 1 cut(s) 1143
BpiI GAAGAC 1 cut(s) 741
BpmI CTGGAG 2 cut(s) 1408, 1436
Bpu14I TTCGAA 2 cut(s) 523, 539
BpuMI CCSGG 1 cut(s) 1219
BsaBI GATNNNNATC 2 cut(s) 267, 1106
BsaI GGTCTC 1 cut(s) 1124
BsaJI CCNNGG 2 cut(s) 150, 1374
BsaXI ACNNNNNCTCC 2 cut(s) 471, 501
Bsc4I CCNNNNNNNGG 3 cut(s) 140, 902, 1166
Bse1I ACTGG 4 cut(s) 803, 1138, 1391, 1419
Bse3DI GCAATG 2 cut(s) 321, 1239
Bse8I GATNNNNATC 2 cut(s) 267, 1106
BseBI CCWGG 2 cut(s) 152, 854
BseDI CCNNGG 2 cut(s) 150, 1374
BseGI GGATG 1 cut(s) 891
BseJI GATNNNNATC 2 cut(s) 267, 1106
BseLI CCNNNNNNNGG 3 cut(s) 140, 902, 1166
BseMI GCAATG 2 cut(s) 321, 1239
BseMII CTCAG 5 cut(s) 141, 175, 233, 873, 1356
BseNI ACTGG 4 cut(s) 803, 1138, 1391, 1419
BseSI GKGCMC 1 cut(s) 205
BseXI GCAGC 4 cut(s) 404, 514, 940, 970
BseYI CCCAGC 1 cut(s) 602
BsgI GTGCAG 3 cut(s) 161, 1049, 1080
BshFI GGCC 3 cut(s) 133, 203, 1166
BsiSI CCGG 4 cut(s) 330, 667, 1167, 1218
BslFI GGGAC 4 cut(s) 133, 658, 817, 1234
BslI CCNNNNNNNGG 3 cut(s) 140, 902, 1166
BsmAI GTCTC 4 cut(s) 130, 694, 1124, 1370
BsmBI CGTCTC 1 cut(s) 1370
BsmFI GGGAC 4 cut(s) 133, 658, 817, 1234
BsmI GAATGC 1 cut(s) 755
BsnI GGCC 3 cut(s) 133, 203, 1166
Bso31I GGTCTC 1 cut(s) 1124
Bsp119I TTCGAA 2 cut(s) 523, 539
Bsp120I GGGCCC 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 205
Bsp143I GATC 5 cut(s) 4, 226, 238, 704, 905
BspACI CCGC 3 cut(s) 517, 700, 752
BspANI GGCC 3 cut(s) 133, 203, 1166
BspCNI CTCAG 5 cut(s) 140, 174, 232, 872, 1355
BspLI GGNNCC 6 cut(s) 148, 149, 203, 334, 1216, 1222
BspPI GGATC 2 cut(s) 12, 913
BspT104I TTCGAA 2 cut(s) 523, 539
BspTNI GGTCTC 1 cut(s) 1124
BsrDI GCAATG 2 cut(s) 321, 1239
BsrI ACTGG 4 cut(s) 803, 1138, 1391, 1419
BssECI CCNNGG 2 cut(s) 150, 1374
BssMI GATC 5 cut(s) 4, 226, 238, 704, 905
BssSI CACGAG 1 cut(s) 1309
BssT1I CCWWGG 1 cut(s) 1374
Bst2BI CACGAG 1 cut(s) 1309
Bst2UI CCWGG 2 cut(s) 152, 854
Bst4CI ACNGT 2 cut(s) 831, 1114
BstAPI GCANNNNNTGC 1 cut(s) 934
BstBI TTCGAA 2 cut(s) 523, 539
BstC8I GCNNGC 3 cut(s) 422, 926, 1236
BstDEI CTNAG 6 cut(s) 127, 161, 219, 531, 859, 1342
BstENI CCTNNNNNAGG 1 cut(s) 138
BstF5I GGATG 1 cut(s) 891
BstKTI GATC 5 cut(s) 7, 229, 241, 707, 908
BstMAI GTCTC 4 cut(s) 130, 694, 1124, 1370
BstMBI GATC 5 cut(s) 4, 226, 238, 704, 905
BstMWI GCNNNNNNNGC 4 cut(s) 934, 1172, 1281, 1400
BstNI CCWGG 2 cut(s) 152, 854
BstNSI RCATGY 1 cut(s) 928
BstSCI CCNGG 3 cut(s) 150, 852, 1217
BstSLI GKGCMC 1 cut(s) 205
BstV1I GCAGC 4 cut(s) 404, 514, 940, 970
BstV2I GAAGAC 1 cut(s) 741
BstX2I RGATCY 2 cut(s) 226, 704
BstYI RGATCY 2 cut(s) 226, 704
BsuI GTATCC 1 cut(s) 792
BsuRI GGCC 3 cut(s) 133, 203, 1166
BtgZI GCGATG 1 cut(s) 666
BtsCI GGATG 1 cut(s) 891
BtsIMutI CAGTG 2 cut(s) 810, 827
Cac8I GCNNGC 3 cut(s) 422, 926, 1236
Cfr13I GGNCC 6 cut(s) 131, 147, 154, 201, 202, 1221
CseI GACGC 2 cut(s) 833, 1352
CviAII CATG 6 cut(s) 272, 623, 729, 925, 1051, 1201
DdeI CTNAG 6 cut(s) 127, 161, 219, 531, 859, 1342
DpnI GATC 5 cut(s) 6, 228, 240, 706, 907
DpnII GATC 5 cut(s) 4, 226, 238, 704, 905
DraI TTTAAA 1 cut(s) 1080
DraIII CACNNNGTG 1 cut(s) 19
DriI GACNNNNNGTC 1 cut(s) 829
EaeI YGGCCR 1 cut(s) 1164
Eam1105I GACNNNNNGTC 1 cut(s) 829
Eco130I CCWWGG 1 cut(s) 1374
Eco24I GRGCYC 1 cut(s) 205
Eco31I GGTCTC 1 cut(s) 1124
Eco47I GGWCC 3 cut(s) 147, 154, 1221
Eco57I CTGAAG 1 cut(s) 1277
EcoNI CCTNNNNNAGG 1 cut(s) 138
EcoO109I RGGNCCY 4 cut(s) 131, 147, 154, 201
EcoRI GAATTC 1 cut(s) 541
EcoRII CCWGG 2 cut(s) 150, 852
EcoT14I CCWWGG 1 cut(s) 1374
EcoT22I ATGCAT 1 cut(s) 1107
EcoT38I GRGCYC 1 cut(s) 205
ErhI CCWWGG 1 cut(s) 1374
Esp3I CGTCTC 1 cut(s) 1370
FaeI CATG 6 cut(s) 275, 626, 732, 928, 1054, 1204
FaqI GGGAC 4 cut(s) 133, 658, 817, 1234
FatI CATG 6 cut(s) 271, 622, 728, 924, 1050, 1200
FauI CCCGC 1 cut(s) 510
FbaI TGATCA 1 cut(s) 238
Fnu4HI GCNGC 5 cut(s) 418, 503, 753, 929, 984
FokI GGATG 1 cut(s) 878
FriOI GRGCYC 1 cut(s) 205
Fsp4HI GCNGC 5 cut(s) 418, 503, 753, 929, 984
GluI GCNGC 5 cut(s) 418, 503, 753, 929, 984
GsaI CCCAGC 1 cut(s) 606
GsuI CTGGAG 2 cut(s) 1408, 1436
HaeIII GGCC 3 cut(s) 133, 203, 1166
HapII CCGG 4 cut(s) 330, 667, 1167, 1218
HgaI GACGC 2 cut(s) 833, 1352
Hin1II CATG 6 cut(s) 275, 626, 732, 928, 1054, 1204
HincII GTYRAC 1 cut(s) 1384
HindII GTYRAC 1 cut(s) 1384
HindIII AAGCTT 1 cut(s) 422
HinfI GANTC 9 cut(s) 263, 460, 725, 857, 1144, 1183, 1265, 1304, 1433
HpaII CCGG 4 cut(s) 330, 667, 1167, 1218
HphI GGTGA 3 cut(s) 383, 586, 593
Hpy166II GTNNAC 2 cut(s) 872, 1384
Hpy188I TCNGA 8 cut(s) 40, 222, 862, 910, 1012, 1048, 1345, 1429
Hpy188III TCNNGA 4 cut(s) 457, 789, 974, 1148
Hpy8I GTNNAC 2 cut(s) 872, 1384
HpyAV CCTTC 6 cut(s) 20, 167, 635, 789, 802, 1252
HpyCH4III ACNGT 2 cut(s) 831, 1114
HpyCH4IV ACGT 2 cut(s) 15, 1399
HpyF10VI GCNNNNNNNGC 4 cut(s) 934, 1172, 1281, 1400
HpyF3I CTNAG 6 cut(s) 127, 161, 219, 531, 859, 1342
HpySE526I ACGT 2 cut(s) 15, 1399
Hsp92II CATG 6 cut(s) 275, 626, 732, 928, 1054, 1204
KflI GGGWCCC 1 cut(s) 147
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 5 cut(s) 4, 226, 238, 704, 905
LmnI GCTCC 2 cut(s) 338, 1360
Lsp1109I GCAGC 4 cut(s) 404, 514, 940, 970
LweI GCATC 2 cut(s) 1052, 1092
MaeII ACGT 2 cut(s) 15, 1399
MaeIII GTNAC 2 cut(s) 581, 1196
MalI GATC 5 cut(s) 6, 228, 240, 706, 907
MboI GATC 5 cut(s) 4, 226, 238, 704, 905
MboII GAAGA 6 cut(s) 53, 482, 682, 746, 804, 962
MflI RGATCY 2 cut(s) 226, 704
MhlI GDGCHC 1 cut(s) 205
MlyI GAGTC 5 cut(s) 719, 851, 1274, 1298, 1427
MnlI CCTC 7 cut(s) 192, 390, 477, 492, 790, 860, 1197
Mph1103I ATGCAT 1 cut(s) 1107
MroXI GAANNNNTTC 1 cut(s) 1143
MseI TTAA 5 cut(s) 566, 711, 848, 1079, 1462
MslI CAYNNNNRTG 1 cut(s) 627
MspI CCGG 4 cut(s) 330, 667, 1167, 1218
MspR9I CCNGG 3 cut(s) 152, 854, 1219
Mva1269I GAATGC 1 cut(s) 755
MvaI CCWGG 2 cut(s) 152, 854
MwoI GCNNNNNNNGC 4 cut(s) 934, 1172, 1281, 1400
NciI CCSGG 1 cut(s) 1219
NdeII GATC 5 cut(s) 4, 226, 238, 704, 905
NlaIII CATG 6 cut(s) 275, 626, 732, 928, 1054, 1204
NlaIV GGNNCC 6 cut(s) 148, 149, 203, 334, 1216, 1222
NmuCI GTSAC 1 cut(s) 581
NsiI ATGCAT 1 cut(s) 1107
NspI RCATGY 1 cut(s) 928
NspV TTCGAA 2 cut(s) 523, 539
PaeI GCATGC 1 cut(s) 928
PctI GAATGC 1 cut(s) 755
PdmI GAANNNNTTC 1 cut(s) 1143
PfeI GAWTC 4 cut(s) 263, 460, 1144, 1183
PfoI TCCNGGA 1 cut(s) 852
PkrI GCNGC 5 cut(s) 419, 504, 754, 930, 985
PleI GAGTC 5 cut(s) 719, 851, 1273, 1298, 1427
PpsI GAGTC 5 cut(s) 719, 851, 1273, 1298, 1427
PpuMI RGGWCCY 2 cut(s) 147, 154
PsiI TTATAA 1 cut(s) 138
Psp5II RGGWCCY 2 cut(s) 147, 154
Psp6I CCWGG 2 cut(s) 150, 852
PspFI CCCAGC 1 cut(s) 602
PspGI CCWGG 2 cut(s) 150, 852
PspN4I GGNNCC 6 cut(s) 148, 149, 203, 334, 1216, 1222
PspOMI GGGCCC 1 cut(s) 201
PspPI GGNCC 6 cut(s) 131, 147, 154, 201, 202, 1221
PspPPI RGGWCCY 2 cut(s) 147, 154
PsuI RGATCY 2 cut(s) 226, 704
RseI CAYNNNNRTG 1 cut(s) 627
SaqAI TTAA 5 cut(s) 566, 711, 848, 1079, 1462
SatI GCNGC 5 cut(s) 418, 503, 753, 929, 984
Sau3AI GATC 5 cut(s) 4, 226, 238, 704, 905
Sau96I GGNCC 6 cut(s) 131, 147, 154, 201, 202, 1221
SchI GAGTC 5 cut(s) 719, 851, 1274, 1298, 1427
ScrFI CCNGG 3 cut(s) 152, 854, 1219
SduI GDGCHC 1 cut(s) 205
SfaNI GCATC 2 cut(s) 1052, 1092
SfuI TTCGAA 2 cut(s) 523, 539
SinI GGWCC 3 cut(s) 147, 154, 1221
SmiI ATTTAAAT 1 cut(s) 1080
SmiMI CAYNNNNRTG 1 cut(s) 627
SphI GCATGC 1 cut(s) 928
SsiI CCGC 3 cut(s) 517, 700, 752
SspI AATATT 1 cut(s) 52
StyD4I CCNGG 3 cut(s) 150, 852, 1217
StyI CCWWGG 1 cut(s) 1374
SwaI ATTTAAAT 1 cut(s) 1080
TaaI ACNGT 2 cut(s) 831, 1114
TaiI ACGT 2 cut(s) 18, 1402
TaqI TCGA 5 cut(s) 281, 523, 539, 545, 1263
TauI GCSGC 1 cut(s) 755
TfiI GAWTC 4 cut(s) 263, 460, 1144, 1183
Tru1I TTAA 5 cut(s) 566, 711, 848, 1079, 1462
Tru9I TTAA 5 cut(s) 566, 711, 848, 1079, 1462
TscAI CASTG 2 cut(s) 810, 834
TseFI GTSAC 1 cut(s) 581
TseI GCWGC 4 cut(s) 417, 502, 928, 983
Tsp45I GTSAC 1 cut(s) 581
TspDTI ATGAA 3 cut(s) 109, 962, 1461
TspGWI ACGGA 1 cut(s) 753
TspRI CASTG 2 cut(s) 810, 834
VpaK11BI GGWCC 3 cut(s) 147, 154, 1221
XagI CCTNNNNNAGG 1 cut(s) 138
XapI RAATTY 2 cut(s) 69, 541
XceI RCATGY 1 cut(s) 928
XmnI GAANNNNTTC 1 cut(s) 1143
Zsp2I ATGCAT 1 cut(s) 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.