MD09G1105400.v1.1

traB domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011100
Physical Location & Seq
Forward (+)
7672732 .. 7676036
3305 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1105400.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGATTCGCTCGACCCGCCTGGTCGCCTCACTCTACTCGCCCGAGTCCCGCCGCGTCTTCCTCTCTCTCCGCCGTTCTCCTAACTCCCTCGCGCAATTCTCCACCGTCAGGAATCTCAGATTTGCTTCCTCTGCCACCGACGGGTGTCCGCGTCAGCGCGAACCGATACGGCCCCTGATGGACGCGGAATCACCTTTGCCCGACCCGGCGTCAGCCGAAAACTTTGTCCACATAGAGAACCCTACGACTGAGGATTTGTCCGATAGCATCGTAGGCGTCGATGCGCCGAGGGATGAGTACGACGACGGCATTTCGATGACCGCCGTGGCTGAGAGTTTCGAACAGAGGAGGTTTCTTCCTGAGGAGCTGTCGAGGAGCGTGGTGGTGCTCACTTGTGAGTCCACGGCTGAGGGCGGCGTTTGCGACGTGCACCTGGTTGGAACGGCACACGTTTCGGTGGAGTCGTGCAGAGAAGTTGAAGCCGTGATCAGCTATTTGAAACCAGAGGTTGTCTTCTTGGAGTTGTGCTCGAGTCGAGTGGCGGCACTCACCCCTCAGAATTTGAAGGTACCGACGATGGGAGAAATGATTGAAATGTGGAAGAAAAAGCACAATGCCTTTGGAATTGTTTACAGCTGGTTCCTTGCAAAGGTTTCAAGTAGGCTTGAAGTGTTTCCTGGTGCTGAGTTTCGTGTAGCGTATGAAGAAGCAATGAAGTATGGCGGTAGGGTGATACTTGGTGATCGCCCCGTGCAGATTACCTTGCGAAGGACGTGGGCAAAGATGCCACTTTGGCATAAGATAAAACTGCTATATTCCTTACTTTTTCAAGCTGTCTTTCTACCAAGTCCCGATGCTCTTAATAGTATGCTTAAGGAAATGGATGATGTTGACATGCTTACTCTTGTGATTCAAGAGATGAGCAAGGAGTACCCAACTCTAATGGAAACACTCGTACATGAGCGAGATCAGTACATGTCGTCTACATTACTGAGAATTGCCAGCGAGCATCGTTCAGTAGTTGCAGTAGTGGGAAAGGGGCACCTCCAAGGAATTAAGAAGCATTGGCAGCAGCCTGTTGTGATGAAGGATCTTATGGTAATTCCATCACAAAAGTCCTTTTTTACCACCGCGAGGATCCTTAAATCTTTGGGTGTCGCGGTAACTGGGGCGGCCATAGTATCAGGCATCTATCTCGCATCCAAGAGGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TraB

Protein Analysis

405

Amino Acids

45.28

Weight (kDa)

7.68

Isoelectric Point (pI)

53.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TraB_PrgY_gumN PF01963 139 - 358 4.3e-23 TraB/PrgY/gumN family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011100)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05270 AT2G32340 AT2G32340
fragaria_vesca FvH4_6g43520 FvH4_6g43520
malus_domestica MD09G1105400.v1.1 MD17G1093000.v1.1
prunus_persica Prupe.3G220000_v2.0.a1
pyrus_communis pycom09g02840 pycom17g09060
rosa_chinensis RchiOBHm_Chr2g0160041
rosa_laevigata RLG00000021193
rosa_multiflora Rmu_sc0002023.1_g000016
rosa_roxburghii Rroxscaffold_2G00090070
rosa_rugosa Rorug02G0479900
rosa_samantha Rh2AG546500 Rh2BG559900 Rh2CG529900 Rh2DG568600
rosa_wichuraiana Rw2G045190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 568
AccB1I GGYRCC 2 cut(s) 568, 1041
AccI GTMKAC 1 cut(s) 983
AccII CGCG 7 cut(s) 54, 92, 151, 159, 185, 1133, 1160
AclWI GGATC 3 cut(s) 1098, 1132, 1145
AcoI YGGCCR 1 cut(s) 1173
AcsI RAATTY 1 cut(s) 559
AcyI GRCGYC 2 cut(s) 209, 276
AfaI GTAC 5 cut(s) 299, 570, 932, 957, 974
AfiI CCNNNNNNNGG 6 cut(s) 108, 141, 578, 649, 768, 1134
AflII CTTAAG 1 cut(s) 872
AflIII ACRYGT 2 cut(s) 448, 975
AgsI TTSAA 8 cut(s) 479, 499, 565, 593, 657, 668, 830, 914
AhdI GACNNNNNGTC 1 cut(s) 208
AjiI CACGTC 2 cut(s) 427, 774
AjnI CCWGG 3 cut(s) 18, 432, 676
AluBI AGCT 4 cut(s) 367, 492, 636, 833
AluI AGCT 4 cut(s) 367, 492, 636, 833
Alw21I GWGCWC 3 cut(s) 390, 432, 530
Alw44I GTGCAC 1 cut(s) 428
AlwI GGATC 3 cut(s) 1098, 1132, 1145
Ama87I CYCGRG 2 cut(s) 41, 529
AoxI GGCC 2 cut(s) 170, 1173
ApaLI GTGCAC 1 cut(s) 428
ApeKI GCWGC 2 cut(s) 1069, 1072
ApoI RAATTY 1 cut(s) 559
ArsI GACNNNNNNTTYG 4 cut(s) 210, 238, 242, 270
Asp700I GAANNNNTTC 1 cut(s) 672
Asp718I GGTACC 1 cut(s) 568
AspLEI GCGC 3 cut(s) 94, 159, 286
AspS9I GGNCC 1 cut(s) 171
AsuC2I CCSGG 1 cut(s) 206
AsuHPI GGTGA 4 cut(s) 183, 541, 742, 752
AsuII TTCGAA 1 cut(s) 339
AvaI CYCGRG 2 cut(s) 41, 529
AxyI CCTNAGG 1 cut(s) 360
BaeGI GKGCMC 2 cut(s) 432, 1044
BamHI GGATCC 1 cut(s) 1137
BanI GGYRCC 2 cut(s) 568, 1041
BbsI GAAGAC 2 cut(s) 49, 505
Bbv12I GWGCWC 3 cut(s) 390, 432, 530
BbvCI CCTCAGC 1 cut(s) 408
BbvI GCAGC 2 cut(s) 1081, 1084
BccI CCATC 3 cut(s) 172, 571, 1114
BceAI ACGGC 7 cut(s) 57, 185, 308, 322, 420, 459, 467
BcgI CGANNNNNNTGC 4 cut(s) 435, 469, 1177, 1211
BciT130I CCWGG 3 cut(s) 20, 434, 678
BclI TGATCA 1 cut(s) 486
BcnI CCSGG 1 cut(s) 206
BfrI CTTAAG 1 cut(s) 872
BglI GCCNNNNNGGC 1 cut(s) 793
BisI GCNGC 6 cut(s) 52, 415, 543, 1070, 1073, 1173
BlsI GCNGC 6 cut(s) 53, 416, 544, 1071, 1074, 1174
Bme1390I CCNGG 4 cut(s) 20, 206, 434, 678
BmeRI GACNNNNNGTC 1 cut(s) 208
BmeT110I CYCGRG 2 cut(s) 41, 529
BmgBI CACGTC 2 cut(s) 427, 774
BmgT120I GGNCC 1 cut(s) 171
BmiI GGNNCC 5 cut(s) 173, 570, 641, 1043, 1139
BmrFI CCNGG 4 cut(s) 20, 206, 434, 678
BmrI ACTGGG 1 cut(s) 1176
BmsI GCATC 7 cut(s) 271, 276, 774, 844, 1018, 1197, 1208
BmuI ACTGGG 1 cut(s) 1176
BoxI GACNNNNGTC 1 cut(s) 144
BpiI GAAGAC 2 cut(s) 49, 505
BplI GAGNNNNNCTC 2 cut(s) 512, 544
Bpu10I CCTNAGC 1 cut(s) 408
Bpu14I TTCGAA 1 cut(s) 339
BpuMI CCSGG 1 cut(s) 206
BsaHI GRCGYC 2 cut(s) 209, 276
BsaJI CCNNGG 4 cut(s) 287, 324, 402, 1048
Bsc4I CCNNNNNNNGG 6 cut(s) 108, 141, 578, 649, 768, 1134
Bse1I ACTGG 1 cut(s) 1171
Bse21I CCTNAGG 1 cut(s) 360
Bse3DI GCAATG 1 cut(s) 717
BseBI CCWGG 3 cut(s) 20, 434, 678
BseDI CCNNGG 4 cut(s) 287, 324, 402, 1048
BseGI GGATG 3 cut(s) 298, 889, 1199
BseLI CCNNNNNNNGG 6 cut(s) 108, 141, 578, 649, 768, 1134
BseMI GCAATG 1 cut(s) 717
BseMII CTCAG 8 cut(s) 130, 240, 321, 351, 399, 569, 675, 983
BseNI ACTGG 1 cut(s) 1171
BseRI GAGGAG 3 cut(s) 361, 377, 388
BseSI GKGCMC 2 cut(s) 432, 1044
BseXI GCAGC 2 cut(s) 1081, 1084
BsgI GTGCAG 2 cut(s) 487, 773
Bsh1236I CGCG 7 cut(s) 54, 92, 151, 159, 185, 1133, 1160
BshFI GGCC 2 cut(s) 172, 1175
BshNI GGYRCC 2 cut(s) 568, 1041
BsiHKAI GWGCWC 3 cut(s) 390, 432, 530
BsiHKCI CYCGRG 2 cut(s) 41, 529
BsiSI CCGG 1 cut(s) 206
BslFI GGGAC 2 cut(s) 31, 834
BslI CCNNNNNNNGG 6 cut(s) 108, 141, 578, 649, 768, 1134
BsmFI GGGAC 2 cut(s) 31, 834
BsnI GGCC 2 cut(s) 172, 1175
BsoBI CYCGRG 2 cut(s) 41, 529
Bsp119I TTCGAA 1 cut(s) 339
Bsp1286I GDGCHC 4 cut(s) 390, 432, 530, 1044
Bsp143I GATC 5 cut(s) 486, 742, 967, 1090, 1137
BspANI GGCC 2 cut(s) 172, 1175
BspCNI CTCAG 8 cut(s) 129, 241, 322, 352, 400, 568, 676, 984
BspFNI CGCG 7 cut(s) 54, 92, 151, 159, 185, 1133, 1160
BspLI GGNNCC 5 cut(s) 173, 570, 641, 1043, 1139
BspPI GGATC 3 cut(s) 1098, 1132, 1145
BspT104I TTCGAA 1 cut(s) 339
BspT107I GGYRCC 2 cut(s) 568, 1041
BspTI CTTAAG 1 cut(s) 872
BsrDI GCAATG 1 cut(s) 717
BsrI ACTGG 1 cut(s) 1171
BssECI CCNNGG 4 cut(s) 287, 324, 402, 1048
BssMI GATC 5 cut(s) 486, 742, 967, 1090, 1137
BssNI GRCGYC 2 cut(s) 209, 276
BssT1I CCWWGG 1 cut(s) 1048
Bst2UI CCWGG 3 cut(s) 20, 434, 678
Bst4CI ACNGT 1 cut(s) 106
BstACI GRCGYC 2 cut(s) 209, 276
BstAFI CTTAAG 1 cut(s) 872
BstBI TTCGAA 1 cut(s) 339
BstC8I GCNNGC 2 cut(s) 1003, 1007
BstDEI CTNAG 8 cut(s) 116, 249, 330, 360, 408, 555, 684, 992
BstDSI CCRYGG 2 cut(s) 324, 402
BstENI CCTNNNNNAGG 2 cut(s) 647, 766
BstF5I GGATG 3 cut(s) 298, 889, 1199
BstFNI CGCG 7 cut(s) 54, 92, 151, 159, 185, 1133, 1160
BstHHI GCGC 3 cut(s) 94, 159, 286
BstKTI GATC 5 cut(s) 489, 745, 970, 1093, 1140
BstMBI GATC 5 cut(s) 486, 742, 967, 1090, 1137
BstMWI GCNNNNNNNGC 6 cut(s) 15, 131, 273, 420, 793, 1069
BstNI CCWGG 3 cut(s) 20, 434, 678
BstNSI RCATGY 2 cut(s) 898, 979
BstPAI GACNNNNGTC 1 cut(s) 144
BstSCI CCNGG 4 cut(s) 18, 204, 432, 676
BstSLI GKGCMC 2 cut(s) 432, 1044
BstUI CGCG 7 cut(s) 54, 92, 151, 159, 185, 1133, 1160
BstV1I GCAGC 2 cut(s) 1081, 1084
BstV2I GAAGAC 2 cut(s) 49, 505
BstX2I RGATCY 2 cut(s) 1090, 1137
BstYI RGATCY 2 cut(s) 1090, 1137
Bsu36I CCTNAGG 1 cut(s) 360
BsuRI GGCC 2 cut(s) 172, 1175
BtgI CCRYGG 2 cut(s) 324, 402
BtrI CACGTC 2 cut(s) 427, 774
BtsCI GGATG 3 cut(s) 298, 889, 1199
Cac8I GCNNGC 2 cut(s) 1003, 1007
CfoI GCGC 3 cut(s) 94, 159, 286
Cfr13I GGNCC 1 cut(s) 171
CseI GACGC 5 cut(s) 43, 140, 191, 198, 265
CsiI ACCWGGT 1 cut(s) 432
Csp6I GTAC 5 cut(s) 298, 569, 931, 956, 973
CviAII CATG 3 cut(s) 895, 959, 976
CviQI GTAC 5 cut(s) 298, 569, 931, 956, 973
DdeI CTNAG 8 cut(s) 116, 249, 330, 360, 408, 555, 684, 992
DpnI GATC 5 cut(s) 488, 744, 969, 1092, 1139
DpnII GATC 5 cut(s) 486, 742, 967, 1090, 1137
DriI GACNNNNNGTC 1 cut(s) 208
EaeI YGGCCR 1 cut(s) 1173
Eam1105I GACNNNNNGTC 1 cut(s) 208
EciI GGCGGA 1 cut(s) 59
Eco130I CCWWGG 1 cut(s) 1048
Eco81I CCTNAGG 1 cut(s) 360
Eco88I CYCGRG 2 cut(s) 41, 529
EcoNI CCTNNNNNAGG 2 cut(s) 647, 766
EcoRII CCWGG 3 cut(s) 18, 432, 676
EcoT14I CCWWGG 1 cut(s) 1048
ErhI CCWWGG 1 cut(s) 1048
FaeI CATG 3 cut(s) 898, 962, 979
FaqI GGGAC 2 cut(s) 31, 834
FatI CATG 3 cut(s) 894, 958, 975
FauI CCCGC 2 cut(s) 23, 56
FbaI TGATCA 1 cut(s) 486
FblI GTMKAC 1 cut(s) 983
Fnu4HI GCNGC 6 cut(s) 52, 415, 543, 1070, 1073, 1173
FokI GGATG 3 cut(s) 305, 896, 1186
Fsp4HI GCNGC 6 cut(s) 52, 415, 543, 1070, 1073, 1173
GlaI GCGC 3 cut(s) 93, 158, 285
GluI GCNGC 6 cut(s) 52, 415, 543, 1070, 1073, 1173
HaeIII GGCC 2 cut(s) 172, 1175
HapII CCGG 1 cut(s) 206
HgaI GACGC 5 cut(s) 43, 140, 191, 198, 265
HhaI GCGC 3 cut(s) 94, 159, 286
Hin1I GRCGYC 2 cut(s) 209, 276
Hin1II CATG 3 cut(s) 898, 962, 979
Hin6I GCGC 3 cut(s) 92, 157, 284
HinP1I GCGC 3 cut(s) 92, 157, 284
HincII GTYRAC 1 cut(s) 892
HindII GTYRAC 1 cut(s) 892
HinfI GANTC 8 cut(s) 4, 44, 112, 188, 398, 461, 532, 910
HpaII CCGG 1 cut(s) 206
HphI GGTGA 4 cut(s) 183, 541, 742, 752
Hpy166II GTNNAC 6 cut(s) 229, 402, 430, 631, 892, 984
Hpy188I TCNGA 3 cut(s) 119, 262, 558
Hpy188III TCNNGA 4 cut(s) 109, 359, 851, 914
Hpy8I GTNNAC 6 cut(s) 229, 402, 430, 631, 892, 984
Hpy99I CGWCG 6 cut(s) 143, 281, 305, 308, 428, 577
HpyAV CCTTC 3 cut(s) 559, 762, 1081
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4IV ACGT 3 cut(s) 426, 450, 773
HpyCH4V TGCA 5 cut(s) 430, 468, 647, 754, 1025
HpyF10VI GCNNNNNNNGC 6 cut(s) 15, 131, 273, 420, 793, 1069
HpyF3I CTNAG 8 cut(s) 116, 249, 330, 360, 408, 555, 684, 992
HpySE526I ACGT 3 cut(s) 426, 450, 773
Hsp92I GRCGYC 2 cut(s) 209, 276
Hsp92II CATG 3 cut(s) 898, 962, 979
HspAI GCGC 3 cut(s) 92, 157, 284
KpnI GGTACC 1 cut(s) 572
Ksp22I TGATCA 1 cut(s) 486
Kzo9I GATC 5 cut(s) 486, 742, 967, 1090, 1137
LmnI GCTCC 2 cut(s) 364, 375
Lsp1109I GCAGC 2 cut(s) 1081, 1084
LweI GCATC 7 cut(s) 271, 276, 774, 844, 1018, 1197, 1208
MabI ACCWGGT 1 cut(s) 432
MaeII ACGT 3 cut(s) 426, 450, 773
MaeIII GTNAC 1 cut(s) 1162
MalI GATC 5 cut(s) 488, 744, 969, 1092, 1139
MboI GATC 5 cut(s) 486, 742, 967, 1090, 1137
MboII GAAGA 5 cut(s) 49, 347, 505, 613, 716
MflI RGATCY 2 cut(s) 1090, 1137
MhlI GDGCHC 4 cut(s) 390, 432, 530, 1044
MluCI AATT 6 cut(s) 95, 559, 624, 996, 1053, 1101
MlyI GAGTC 4 cut(s) 53, 407, 470, 541
MmeI TCCRAC 1 cut(s) 418
MroXI GAANNNNTTC 1 cut(s) 672
MseI TTAA 4 cut(s) 861, 873, 1056, 1143
MslI CAYNNNNRTG 1 cut(s) 314
MspA1I CMGCKG 1 cut(s) 636
MspCI CTTAAG 1 cut(s) 872
MspI CCGG 1 cut(s) 206
MspR9I CCNGG 4 cut(s) 20, 206, 434, 678
MvaI CCWGG 3 cut(s) 20, 434, 678
MvnI CGCG 7 cut(s) 54, 92, 151, 159, 185, 1133, 1160
MwoI GCNNNNNNNGC 6 cut(s) 15, 131, 273, 420, 793, 1069
NciI CCSGG 1 cut(s) 206
NdeII GATC 5 cut(s) 486, 742, 967, 1090, 1137
NlaIII CATG 3 cut(s) 898, 962, 979
NlaIV GGNNCC 5 cut(s) 173, 570, 641, 1043, 1139
NmeAIII GCCGAG 1 cut(s) 312
NspI RCATGY 2 cut(s) 898, 979
NspV TTCGAA 1 cut(s) 339
PaeR7I CTCGAG 1 cut(s) 529
PciI ACATGT 1 cut(s) 975
PcsI WCGNNNNNNNCGW 2 cut(s) 276, 461
PdmI GAANNNNTTC 1 cut(s) 672
PfeI GAWTC 4 cut(s) 4, 112, 188, 910
PkrI GCNGC 6 cut(s) 53, 416, 544, 1071, 1074, 1174
PleI GAGTC 4 cut(s) 52, 406, 469, 540
PpsI GAGTC 4 cut(s) 52, 406, 469, 540
PscI ACATGT 1 cut(s) 975
PshAI GACNNNNGTC 1 cut(s) 144
Psp6I CCWGG 3 cut(s) 18, 432, 676
PspGI CCWGG 3 cut(s) 18, 432, 676
PspN4I GGNNCC 5 cut(s) 173, 570, 641, 1043, 1139
PspPI GGNCC 1 cut(s) 171
PspXI VCTCGAGB 1 cut(s) 529
PsuI RGATCY 2 cut(s) 1090, 1137
PvuII CAGCTG 1 cut(s) 636
RsaI GTAC 5 cut(s) 299, 570, 932, 957, 974
RsaNI GTAC 5 cut(s) 298, 569, 931, 956, 973
RseI CAYNNNNRTG 1 cut(s) 314
SaqAI TTAA 4 cut(s) 861, 873, 1056, 1143
SatI GCNGC 6 cut(s) 52, 415, 543, 1070, 1073, 1173
Sau3AI GATC 5 cut(s) 486, 742, 967, 1090, 1137
Sau96I GGNCC 1 cut(s) 171
SchI GAGTC 4 cut(s) 53, 407, 470, 541
ScrFI CCNGG 4 cut(s) 20, 206, 434, 678
SduI GDGCHC 4 cut(s) 390, 432, 530, 1044
SexAI ACCWGGT 1 cut(s) 432
SfaNI GCATC 7 cut(s) 271, 276, 774, 844, 1018, 1197, 1208
Sfr274I CTCGAG 1 cut(s) 529
SfuI TTCGAA 1 cut(s) 339
SlaI CTCGAG 1 cut(s) 529
SmiMI CAYNNNNRTG 1 cut(s) 314
SmlI CTYRAG 2 cut(s) 529, 872
SmoI CTYRAG 2 cut(s) 529, 872
Sse9I AATT 6 cut(s) 95, 559, 624, 996, 1053, 1101
StyD4I CCNGG 4 cut(s) 18, 204, 432, 676
StyI CCWWGG 1 cut(s) 1048
TaaI ACNGT 1 cut(s) 106
TaiI ACGT 3 cut(s) 429, 453, 776
TaqI TCGA 7 cut(s) 11, 279, 314, 339, 371, 530, 535
TasI AATT 6 cut(s) 95, 559, 624, 996, 1053, 1101
TatI WGTACW 1 cut(s) 972
TauI GCSGC 4 cut(s) 54, 417, 545, 1175
TfiI GAWTC 4 cut(s) 4, 112, 188, 910
Tru1I TTAA 4 cut(s) 861, 873, 1056, 1143
Tru9I TTAA 4 cut(s) 861, 873, 1056, 1143
TseI GCWGC 2 cut(s) 1069, 1072
TspDTI ATGAA 3 cut(s) 717, 728, 1100
Vha464I CTTAAG 1 cut(s) 872
VneI GTGCAC 1 cut(s) 428
XagI CCTNNNNNAGG 2 cut(s) 647, 766
XapI RAATTY 1 cut(s) 559
XceI RCATGY 2 cut(s) 898, 979
XhoI CTCGAG 1 cut(s) 529
XmiI GTMKAC 1 cut(s) 983
XmnI GAANNNNTTC 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.