MD17G1093000.v1.1

traB domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011100
Physical Location & Seq
Forward (+)
7795400 .. 7798925
3526 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1093000.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGATTCGCTCGACTCGCCTGATCGCCACACTCTACTCGCCCGAGTCACGCCACGTCTTCCTCTCTCTCCGCCGCTCTCCGAACACCCTCGCGCAATTCTCCACCGTAAGGAATCTCAGATTTGCAGCCACTGCCACCGCCGGCGGTCCGCGTCAGCGCGACCCGATACGGCTCCTGATGGATGCGGAATCACCCTTGCCCGACCCGGCGGCATCCGAAAACTTTGTCCACGTCGGGAACCCTACGATTGGGGACTTGGCCGATAGCATCGTCGGTGTCGATGCGCCGAGGGACGAGTACGACGACAATGTTTCGACGACCGCCGAGGCTGAGAGTTCCGAACAGAGGAGGTTTCTTCCTGAGGAGCTGTCGAGGAGCGTCGTCGTGCTCACTTGTGAGTCCACGGCTGAGGGCGGCGTTTGCGACGTGCACCTGGTTGGAACGGCTCATGTTTCGGTGGAATCGTGCAGAGAAGTTGAAGCAGTGATCAGCTATTTGAAACCACAGGTTGTGTTCTTGGAGTTATGCTCGAGTCGAGTGACTGCACTCACCCCTCAGAATTTGAAGGTGCCGACAATGGGAGAAATGATCGAAATGTGGAAGAAAAAGCATAATGCCTTTGGGATTGTTTACAGCTGGTTCCTTGCCAAGGTTTCAAGTAGGCTTGAGGTGTTTCCCGGTGCTGAGTTTCGTGTAGCATATGAAGAAGCAATGAAGTATGGCGGTAGGGTGATACTTGGTGATCGCCCAGTGCAGATTACGATACGAAGGACGTGGGCAAAAATGCCACTTTGGCATAAGATAAAACTGCTGTATTCCTTGCTTTTTCAAGCAGTCTTTCTACCCAGCCCTGATGCTCTTAATAATATGTTAAAGGAAATGGATGATGTTGACATGCTGACTCTCGTGATTCAAGAGATGAGCAAGGAGTACCCAACTCTAATGGAAACACTCGTACATGAACGAGATCAGTACATGTCATCAACATTACTGAGAATTGCGAGCGAGAATCGTTCAGTAGTTGCAGTAGTGGGAAAGGGGCACCTCCAAGGAATTAAGAAGCATTGGCAGCAGCCTGTTGTGGTGAAGGATCTTTTGGTAATTCCATCAGAAAGGTCAGTTTTTTCCACCCGGAGAATCGTTAAATCTATGGGTGTGGCGGTGACTGGGGCGGCCATAGTATCAGGCATCTATCTTGCATCCAAGAGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TraB

Protein Analysis

405

Amino Acids

45.07

Weight (kDa)

8.55

Isoelectric Point (pI)

50.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TraB_PrgY_gumN PF01963 139 - 358 3.4e-23 TraB/PrgY/gumN family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011100)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05270 AT2G32340 AT2G32340
fragaria_vesca FvH4_6g43520 FvH4_6g43520
malus_domestica MD09G1105400.v1.1 MD17G1093000.v1.1
prunus_persica Prupe.3G220000_v2.0.a1
pyrus_communis pycom09g02840 pycom17g09060
rosa_chinensis RchiOBHm_Chr2g0160041
rosa_laevigata RLG00000021193
rosa_multiflora Rmu_sc0002023.1_g000016
rosa_roxburghii Rroxscaffold_2G00090070
rosa_rugosa Rorug02G0479900
rosa_samantha Rh2AG546500 Rh2BG559900 Rh2CG529900 Rh2DG568600
rosa_wichuraiana Rw2G045190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 568, 1041
AccBSI CCGCTC 1 cut(s) 75
AccII CGCG 3 cut(s) 92, 151, 159
AclWI GGATC 1 cut(s) 1098
AcoI YGGCCR 2 cut(s) 258, 1173
AcsI RAATTY 1 cut(s) 559
AfaI GTAC 4 cut(s) 299, 932, 957, 974
AfiI CCNNNNNNNGG 5 cut(s) 108, 248, 345, 578, 649
AflIII ACRYGT 1 cut(s) 975
AgsI TTSAA 6 cut(s) 479, 499, 565, 657, 830, 914
AjiI CACGTC 4 cut(s) 55, 232, 427, 774
AjnI CCWGG 1 cut(s) 432
AjuI GAANNNNNNNTTGG 2 cut(s) 1079, 1111
AluBI AGCT 3 cut(s) 367, 492, 636
AluI AGCT 3 cut(s) 367, 492, 636
Alw21I GWGCWC 2 cut(s) 390, 432
Alw44I GTGCAC 1 cut(s) 428
AlwI GGATC 1 cut(s) 1098
AlwNI CAGNNNCTG 1 cut(s) 131
Ama87I CYCGRG 2 cut(s) 41, 529
AoxI GGCC 2 cut(s) 258, 1173
ApaLI GTGCAC 1 cut(s) 428
ApeKI GCWGC 3 cut(s) 125, 1069, 1072
ApoI RAATTY 1 cut(s) 559
ArsI GACNNNNNNTTYG 2 cut(s) 210, 242
AspLEI GCGC 3 cut(s) 94, 159, 286
AspS9I GGNCC 1 cut(s) 146
AsuC2I CCSGG 3 cut(s) 206, 678, 1132
AsuHPI GGTGA 6 cut(s) 183, 541, 742, 752, 1096, 1174
AvaI CYCGRG 2 cut(s) 41, 529
AvaII GGWCC 1 cut(s) 146
AxyI CCTNAGG 1 cut(s) 360
BaeGI GKGCMC 2 cut(s) 432, 1044
BanI GGYRCC 2 cut(s) 568, 1041
BauI CACGAG 1 cut(s) 905
BbsI GAAGAC 1 cut(s) 49
Bbv12I GWGCWC 2 cut(s) 390, 432
BbvCI CCTCAGC 1 cut(s) 408
BbvI GCAGC 3 cut(s) 137, 1081, 1084
BccI CCATC 2 cut(s) 172, 1114
BceAI ACGGC 3 cut(s) 185, 420, 459
BciT130I CCWGG 1 cut(s) 434
BclI TGATCA 1 cut(s) 486
BcnI CCSGG 3 cut(s) 206, 678, 1132
BglI GCCNNNNNGGC 1 cut(s) 793
BisI GCNGC 7 cut(s) 73, 126, 210, 415, 1070, 1073, 1173
BlsI GCNGC 7 cut(s) 74, 127, 211, 416, 1071, 1074, 1174
Bme1390I CCNGG 4 cut(s) 206, 434, 678, 1132
Bme18I GGWCC 1 cut(s) 146
BmeT110I CYCGRG 2 cut(s) 41, 529
BmgBI CACGTC 4 cut(s) 55, 232, 427, 774
BmgT120I GGNCC 1 cut(s) 146
BmiI GGNNCC 5 cut(s) 173, 239, 570, 641, 1043
BmrFI CCNGG 4 cut(s) 206, 434, 678, 1132
BmrI ACTGGG 2 cut(s) 743, 1176
BmsI GCATC 7 cut(s) 172, 221, 271, 276, 844, 1197, 1208
BmuI ACTGGG 2 cut(s) 743, 1176
BpiI GAAGAC 1 cut(s) 49
BplI GAGNNNNNCTC 2 cut(s) 512, 544
Bpu10I CCTNAGC 1 cut(s) 408
BpuEI CTTGAG 1 cut(s) 686
BpuMI CCSGG 3 cut(s) 206, 678, 1132
BsaJI CCNNGG 5 cut(s) 287, 324, 402, 648, 1048
Bsc4I CCNNNNNNNGG 5 cut(s) 108, 248, 345, 578, 649
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 2 cut(s) 749, 1171
Bse21I CCTNAGG 1 cut(s) 360
Bse3DI GCAATG 1 cut(s) 717
BseBI CCWGG 1 cut(s) 434
BseDI CCNNGG 5 cut(s) 287, 324, 402, 648, 1048
BseGI GGATG 4 cut(s) 187, 212, 889, 1199
BseLI CCNNNNNNNGG 5 cut(s) 108, 248, 345, 578, 649
BseMI GCAATG 1 cut(s) 717
BseMII CTCAG 7 cut(s) 130, 321, 351, 399, 569, 675, 983
BseNI ACTGG 2 cut(s) 749, 1171
BseRI GAGGAG 3 cut(s) 361, 377, 388
BseSI GKGCMC 2 cut(s) 432, 1044
BseXI GCAGC 3 cut(s) 137, 1081, 1084
BseYI CCCAGC 1 cut(s) 845
BsgI GTGCAG 3 cut(s) 487, 528, 773
Bsh1236I CGCG 3 cut(s) 92, 151, 159
Bsh1285I CGRYCG 1 cut(s) 321
BshFI GGCC 2 cut(s) 260, 1175
BshNI GGYRCC 2 cut(s) 568, 1041
BsiEI CGRYCG 1 cut(s) 321
BsiHKAI GWGCWC 2 cut(s) 390, 432
BsiHKCI CYCGRG 2 cut(s) 41, 529
BsiSI CCGG 4 cut(s) 141, 206, 678, 1132
BslFI GGGAC 2 cut(s) 266, 305
BslI CCNNNNNNNGG 5 cut(s) 108, 248, 345, 578, 649
BsmFI GGGAC 2 cut(s) 266, 305
BsnI GGCC 2 cut(s) 260, 1175
BsoBI CYCGRG 2 cut(s) 41, 529
Bsp1286I GDGCHC 3 cut(s) 390, 432, 1044
Bsp143I GATC 6 cut(s) 21, 486, 588, 742, 967, 1090
BspANI GGCC 2 cut(s) 260, 1175
BspCNI CTCAG 7 cut(s) 129, 322, 352, 400, 568, 676, 984
BspFNI CGCG 3 cut(s) 92, 151, 159
BspLI GGNNCC 5 cut(s) 173, 239, 570, 641, 1043
BspPI GGATC 1 cut(s) 1098
BspT107I GGYRCC 2 cut(s) 568, 1041
BsrBI CCGCTC 1 cut(s) 75
BsrDI GCAATG 1 cut(s) 717
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 2 cut(s) 749, 1171
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 5 cut(s) 287, 324, 402, 648, 1048
BssMI GATC 6 cut(s) 21, 486, 588, 742, 967, 1090
BssSI CACGAG 1 cut(s) 905
BssT1I CCWWGG 2 cut(s) 648, 1048
Bst2BI CACGAG 1 cut(s) 905
Bst2UI CCWGG 1 cut(s) 434
Bst4CI ACNGT 1 cut(s) 106
BstAPI GCANNNNNTGC 1 cut(s) 131
BstC8I GCNNGC 2 cut(s) 142, 1003
BstDEI CTNAG 7 cut(s) 116, 330, 360, 408, 555, 684, 992
BstDSI CCRYGG 1 cut(s) 402
BstENI CCTNNNNNAGG 1 cut(s) 647
BstF5I GGATG 4 cut(s) 187, 212, 889, 1199
BstFNI CGCG 3 cut(s) 92, 151, 159
BstHHI GCGC 3 cut(s) 94, 159, 286
BstKTI GATC 6 cut(s) 24, 489, 591, 745, 970, 1093
BstMBI GATC 6 cut(s) 21, 486, 588, 742, 967, 1090
BstMCI CGRYCG 1 cut(s) 321
BstMWI GCNNNNNNNGC 5 cut(s) 15, 131, 420, 793, 1069
BstNI CCWGG 1 cut(s) 434
BstNSI RCATGY 2 cut(s) 898, 979
BstSCI CCNGG 4 cut(s) 204, 432, 676, 1130
BstSLI GKGCMC 2 cut(s) 432, 1044
BstUI CGCG 3 cut(s) 92, 151, 159
BstV1I GCAGC 3 cut(s) 137, 1081, 1084
BstV2I GAAGAC 1 cut(s) 49
BstX2I RGATCY 1 cut(s) 1090
BstYI RGATCY 1 cut(s) 1090
Bsu36I CCTNAGG 1 cut(s) 360
BsuRI GGCC 2 cut(s) 260, 1175
BtgI CCRYGG 1 cut(s) 402
BtrI CACGTC 4 cut(s) 55, 232, 427, 774
BtsCI GGATG 4 cut(s) 187, 212, 889, 1199
BtsI GCAGTG 2 cut(s) 129, 489
BtsIMutI CAGTG 3 cut(s) 129, 489, 756
Cac8I GCNNGC 2 cut(s) 142, 1003
CaiI CAGNNNCTG 1 cut(s) 131
CfoI GCGC 3 cut(s) 94, 159, 286
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 146
CpoI CGGWCCG 1 cut(s) 146
CseI GACGC 2 cut(s) 140, 367
CsiI ACCWGGT 1 cut(s) 432
Csp6I GTAC 4 cut(s) 298, 931, 956, 973
CspI CGGWCCG 1 cut(s) 146
CviAII CATG 4 cut(s) 449, 895, 959, 976
CviQI GTAC 4 cut(s) 298, 931, 956, 973
DdeI CTNAG 7 cut(s) 116, 330, 360, 408, 555, 684, 992
DpnI GATC 6 cut(s) 23, 488, 590, 744, 969, 1092
DpnII GATC 6 cut(s) 21, 486, 588, 742, 967, 1090
EaeI YGGCCR 2 cut(s) 258, 1173
EciI GGCGGA 1 cut(s) 59
Eco130I CCWWGG 2 cut(s) 648, 1048
Eco47I GGWCC 1 cut(s) 146
Eco81I CCTNAGG 1 cut(s) 360
Eco88I CYCGRG 2 cut(s) 41, 529
EcoNI CCTNNNNNAGG 1 cut(s) 647
EcoRII CCWGG 1 cut(s) 432
EcoT14I CCWWGG 2 cut(s) 648, 1048
ErhI CCWWGG 2 cut(s) 648, 1048
FaeI CATG 4 cut(s) 452, 898, 962, 979
FaqI GGGAC 2 cut(s) 266, 305
FatI CATG 4 cut(s) 448, 894, 958, 975
FauNDI CATATG 1 cut(s) 700
FbaI TGATCA 1 cut(s) 486
Fnu4HI GCNGC 7 cut(s) 73, 126, 210, 415, 1070, 1073, 1173
FokI GGATG 4 cut(s) 194, 199, 896, 1186
Fsp4HI GCNGC 7 cut(s) 73, 126, 210, 415, 1070, 1073, 1173
GlaI GCGC 3 cut(s) 93, 158, 285
GluI GCNGC 7 cut(s) 73, 126, 210, 415, 1070, 1073, 1173
GsaI CCCAGC 1 cut(s) 849
HaeIII GGCC 2 cut(s) 260, 1175
HapII CCGG 4 cut(s) 141, 206, 678, 1132
HgaI GACGC 2 cut(s) 140, 367
HhaI GCGC 3 cut(s) 94, 159, 286
Hin1II CATG 4 cut(s) 452, 898, 962, 979
Hin6I GCGC 3 cut(s) 92, 157, 284
HinP1I GCGC 3 cut(s) 92, 157, 284
HincII GTYRAC 1 cut(s) 892
HindII GTYRAC 1 cut(s) 892
HpaII CCGG 4 cut(s) 141, 206, 678, 1132
HphI GGTGA 6 cut(s) 183, 541, 742, 752, 1096, 1174
Hpy166II GTNNAC 5 cut(s) 229, 402, 430, 631, 892
Hpy188I TCNGA 6 cut(s) 81, 119, 217, 340, 558, 1111
Hpy188III TCNNGA 5 cut(s) 175, 235, 359, 907, 914
Hpy8I GTNNAC 5 cut(s) 229, 402, 430, 631, 892
Hpy99I CGWCG 7 cut(s) 236, 275, 305, 319, 383, 386, 428
HpyAV CCTTC 3 cut(s) 559, 762, 1081
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4IV ACGT 4 cut(s) 54, 231, 426, 773
HpyCH4V TGCA 7 cut(s) 125, 430, 468, 545, 754, 1025, 1199
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 131, 420, 793, 1069
HpyF3I CTNAG 7 cut(s) 116, 330, 360, 408, 555, 684, 992
HpySE526I ACGT 4 cut(s) 54, 231, 426, 773
Hsp92II CATG 4 cut(s) 452, 898, 962, 979
HspAI GCGC 3 cut(s) 92, 157, 284
KroI GCCGGC 1 cut(s) 140
KroNI GCCGGC 1 cut(s) 142
Ksp22I TGATCA 1 cut(s) 486
Kzo9I GATC 6 cut(s) 21, 486, 588, 742, 967, 1090
LmnI GCTCC 3 cut(s) 177, 364, 375
Lsp1109I GCAGC 3 cut(s) 137, 1081, 1084
LweI GCATC 7 cut(s) 172, 221, 271, 276, 844, 1197, 1208
MabI ACCWGGT 1 cut(s) 432
MaeII ACGT 4 cut(s) 54, 231, 426, 773
MaeIII GTNAC 3 cut(s) 45, 538, 1162
MalI GATC 6 cut(s) 23, 488, 590, 744, 969, 1092
MbiI CCGCTC 1 cut(s) 75
MboI GATC 6 cut(s) 21, 486, 588, 742, 967, 1090
MboII GAAGA 4 cut(s) 49, 347, 613, 716
MflI RGATCY 1 cut(s) 1090
MhlI GDGCHC 3 cut(s) 390, 432, 1044
MluCI AATT 5 cut(s) 95, 559, 996, 1053, 1101
MlyI GAGTC 5 cut(s) 7, 53, 407, 541, 895
MmeI TCCRAC 1 cut(s) 418
MreI CGCCGGCG 1 cut(s) 140
MroNI GCCGGC 1 cut(s) 140
MseI TTAA 4 cut(s) 861, 872, 1056, 1143
MspA1I CMGCKG 1 cut(s) 636
MspI CCGG 4 cut(s) 141, 206, 678, 1132
MspR9I CCNGG 4 cut(s) 206, 434, 678, 1132
MvaI CCWGG 1 cut(s) 434
MvnI CGCG 3 cut(s) 92, 151, 159
MwoI GCNNNNNNNGC 5 cut(s) 15, 131, 420, 793, 1069
NaeI GCCGGC 1 cut(s) 142
NciI CCSGG 3 cut(s) 206, 678, 1132
NdeI CATATG 1 cut(s) 700
NdeII GATC 6 cut(s) 21, 486, 588, 742, 967, 1090
NgoMIV GCCGGC 1 cut(s) 140
NlaIII CATG 4 cut(s) 452, 898, 962, 979
NlaIV GGNNCC 5 cut(s) 173, 239, 570, 641, 1043
NmeAIII GCCGAG 2 cut(s) 312, 349
NmuCI GTSAC 3 cut(s) 45, 538, 1162
NspI RCATGY 2 cut(s) 898, 979
PaeR7I CTCGAG 1 cut(s) 529
PciI ACATGT 1 cut(s) 975
PcsI WCGNNNNNNNCGW 3 cut(s) 276, 300, 461
PdiI GCCGGC 1 cut(s) 142
PfeI GAWTC 7 cut(s) 4, 112, 188, 461, 910, 1009, 1137
PkrI GCNGC 7 cut(s) 74, 127, 211, 416, 1071, 1074, 1174
PleI GAGTC 5 cut(s) 7, 52, 406, 540, 895
PpsI GAGTC 5 cut(s) 7, 52, 406, 540, 895
PscI ACATGT 1 cut(s) 975
Psp6I CCWGG 1 cut(s) 432
PspFI CCCAGC 1 cut(s) 845
PspGI CCWGG 1 cut(s) 432
PspN4I GGNNCC 5 cut(s) 173, 239, 570, 641, 1043
PspPI GGNCC 1 cut(s) 146
PspXI VCTCGAGB 1 cut(s) 529
PstNI CAGNNNCTG 1 cut(s) 131
PsuI RGATCY 1 cut(s) 1090
PvuII CAGCTG 1 cut(s) 636
RsaI GTAC 4 cut(s) 299, 932, 957, 974
RsaNI GTAC 4 cut(s) 298, 931, 956, 973
Rsr2I CGGWCCG 1 cut(s) 146
RsrII CGGWCCG 1 cut(s) 146
SaqAI TTAA 4 cut(s) 861, 872, 1056, 1143
SatI GCNGC 7 cut(s) 73, 126, 210, 415, 1070, 1073, 1173
Sau3AI GATC 6 cut(s) 21, 486, 588, 742, 967, 1090
Sau96I GGNCC 1 cut(s) 146
SchI GAGTC 5 cut(s) 7, 53, 407, 541, 895
ScrFI CCNGG 4 cut(s) 206, 434, 678, 1132
SduI GDGCHC 3 cut(s) 390, 432, 1044
SexAI ACCWGGT 1 cut(s) 432
SfaNI GCATC 7 cut(s) 172, 221, 271, 276, 844, 1197, 1208
Sfr274I CTCGAG 1 cut(s) 529
SgrAI CRCCGGYG 1 cut(s) 140
SinI GGWCC 1 cut(s) 146
SlaI CTCGAG 1 cut(s) 529
SmlI CTYRAG 2 cut(s) 529, 665
SmoI CTYRAG 2 cut(s) 529, 665
Sse9I AATT 5 cut(s) 95, 559, 996, 1053, 1101
StyD4I CCNGG 4 cut(s) 204, 432, 676, 1130
StyI CCWWGG 2 cut(s) 648, 1048
TaaI ACNGT 1 cut(s) 106
TaiI ACGT 4 cut(s) 57, 234, 429, 776
TaqI TCGA 7 cut(s) 11, 279, 314, 371, 530, 535, 591
TasI AATT 5 cut(s) 95, 559, 996, 1053, 1101
TatI WGTACW 1 cut(s) 972
TauI GCSGC 4 cut(s) 75, 212, 417, 1175
TfiI GAWTC 7 cut(s) 4, 112, 188, 461, 910, 1009, 1137
Tru1I TTAA 4 cut(s) 861, 872, 1056, 1143
Tru9I TTAA 4 cut(s) 861, 872, 1056, 1143
TscAI CASTG 3 cut(s) 136, 489, 756
TseFI GTSAC 3 cut(s) 45, 538, 1162
TseI GCWGC 3 cut(s) 125, 1069, 1072
Tsp45I GTSAC 3 cut(s) 45, 538, 1162
TspDTI ATGAA 3 cut(s) 717, 728, 975
TspRI CASTG 3 cut(s) 136, 489, 756
VneI GTGCAC 1 cut(s) 428
VpaK11BI GGWCC 1 cut(s) 146
XagI CCTNNNNNAGG 1 cut(s) 647
XapI RAATTY 1 cut(s) 559
XceI RCATGY 2 cut(s) 898, 979
XhoI CTCGAG 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.