MD09G1113400.v1.1

nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004561
Physical Location & Seq
Forward (+)
8612753 .. 8617544
4792 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1113400.v1.1.491

Sequence Viewer

Length: 1563 bp
ATGGCTGACATTACCCACCCTCCCATGGATCAGCTCCAAGACCTGGAGTACTGCATAGACTCCAACCCTCCTTGGGCTGAAACCATACTTCTAGCATTTCAAAACTACATCATGATGCTTGGTACGAGTGTAATGATTCCTTCGGCGCTGGTCCCTGCAATGGGAGGAAGTGATGGTGACAAAGCACGAGTCATACAAACGCTACTCTTTGTTGCTGGAATTAACACCCTTCTCCAAGCGCTTTTCGGAACCAGGTTGCCTGCGGTAGTTGGGGGGTCCTTTTCTTATGTCATTCCCATAGCTTATATTGTGAGCGACCACAAATTGCAACGGATTGCTGAACCTCATGAAAGATTTATACAAACAATGCGTGCCATCCAAGGAGCTCTAATTGTAGCCTCCAGCATACAAATCATTCTGGGTTACAGCCAAGTTTGGGGTCTTTTCTCAAGGTTTTTCAGTCCACTTGGCATGGCACCTGTAGTTGGATTGGTTGGCTTGGGATTGTTTGAACGGGGTTTTCCTGCGTTGGGAAATTGTGTGGAAATTGGAATTCCGATGCTGTTGTTGGTAATTGGGTTGTCTCAATATCTGAAGCATGTGAGACCATTTAGAGATGTCCCTATTTTTGAACGGTTTCCAGTATTGTTTTGTGTCACGATCATTTGGATCTATTCCCTTATCTTAACCGCCGGTGGGGCTTACCGTGACAAGCCTGTTAGAACCCAAATCAGCTGCCGCACAGACAGGGAAAATCTCATATCTACTGCCCCATGGTTCAAGTTCCCATATCCTCTGCAGTGGGGTCCTCCTACATTTTCAGCAGGTCACTCATGTGCAATGATGTCCGCAGTTCTTGTTTCAATGGTTGAGTCAACTGCTGCGTACAAGGCAGCATCAAGATTGGCAATTGCCACTCCTCCTCCTGCTTATGTATTGAGCCGGGGAATTGGCTGGCAGGGCATCGGTATTTTGCTTGACGGACTTTTTGGTACGGGCACTGGTTCTACTGTTTCTGTGGAAAATGTCGGACTTCTTGGACTAACCCGAGTTGGAAGTCGTAGAGTTGTTCAAATTTCTGCTGCATTCATGATATTCTTCTCAACCTTAGGTAAATTTGGGGCTGTATTTGCATCGATACCCTTCCCGATATATGCAGCTTTATACTGTGTTCTCTTCGGCCTCGTGGCTTCGGTAGGGTTATCATTTCTTCAATTCACAAACATGAATTCTATGAGAAACCTCATCATCACCGGGCTCTCTCTCTTCCTTGGGATATCTGTCCCTCGCTTTTTCAACGAATATTGGAACCCCTCGCACCATGGTCTCGTCCATACCAATGCTGGATGGTTCAATGCATTCCTGAACACTATATTCTCATCTCCTCCAACCGTGGGGCTAATCGTGGCGGTGTTTCTTGACAACACTTTAGAGGTGGAAAAATCGAAGAAGGACAGAGGGATGCCGTGGTGGGTTAAGTTCAGAACATTCAGAGGAGACAACAGAAATGAAGAGTTCTACACGTTGCCGTTTAACCTCAACAGATTCTTCCCGCCTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0009506 GO:0030054 GO:0055044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Xan_ur_permease

Protein Analysis

521

Amino Acids

57.29

Weight (kDa)

9.17

Isoelectric Point (pI)

29.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 27 - 431 1.4e-65 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 815
AccB1I GGYRCC 1 cut(s) 475
AccB7I CCANNNNNTGG 1 cut(s) 43
AciI CCGC 6 cut(s) 263, 690, 739, 849, 1409, 1553
AclWI GGATC 2 cut(s) 36, 677
AcsI RAATTY 4 cut(s) 552, 1074, 1115, 1228
AcuI CTGAAG 1 cut(s) 614
AfaI GTAC 4 cut(s) 50, 124, 887, 994
AfeI AGCGCT 1 cut(s) 240
AflIII ACRYGT 1 cut(s) 1521
AgsI TTSAA 9 cut(s) 101, 512, 632, 781, 864, 1073, 1214, 1297, 1354
AjnI CCWGG 2 cut(s) 42, 251
AleI CACNNNNGTG 1 cut(s) 834
AluBI AGCT 5 cut(s) 34, 302, 386, 735, 1160
AluI AGCT 5 cut(s) 34, 302, 386, 735, 1160
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 4 cut(s) 588, 598, 1331, 1491
AlwI GGATC 2 cut(s) 36, 677
Ama87I CYCGRG 1 cut(s) 1047
Aor51HI AGCGCT 1 cut(s) 240
AoxI GGCC 1 cut(s) 1180
ApeKI GCWGC 5 cut(s) 735, 881, 893, 1082, 1157
ApoI RAATTY 4 cut(s) 552, 1074, 1115, 1228
ArsI GACNNNNNNTTYG 4 cut(s) 174, 206, 971, 1003
Asp700I GAANNNNTTC 1 cut(s) 636
AspLEI GCGC 2 cut(s) 148, 241
AspS9I GGNCC 3 cut(s) 151, 276, 806
AsuC2I CCSGG 2 cut(s) 944, 1255
AsuHPI GGTGA 2 cut(s) 188, 1243
AvaI CYCGRG 1 cut(s) 1047
AvaII GGWCC 3 cut(s) 151, 276, 806
AxyI CCTNAGG 1 cut(s) 1108
BaeGI GKGCMC 1 cut(s) 1001
BaeI ACNNNNGTAYC 2 cut(s) 114, 147
BanI GGYRCC 1 cut(s) 475
BanII GRGCYC 2 cut(s) 388, 1260
BauI CACGAG 2 cut(s) 186, 1184
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 5 cut(s) 722, 868, 905, 1069, 1169
BccI CCATC 3 cut(s) 167, 383, 1341
BceAI ACGGC 2 cut(s) 1450, 1513
BciT130I CCWGG 2 cut(s) 44, 253
BcnI CCSGG 2 cut(s) 944, 1255
BcoDI GTCTC 4 cut(s) 588, 598, 1331, 1491
BfaI CTAG 1 cut(s) 92
BfmI CTRYAG 2 cut(s) 480, 797
BfoI RGCGCY 2 cut(s) 149, 242
BfuAI ACCTGC 1 cut(s) 815
BglI GCCNNNNNGGC 1 cut(s) 698
BisI GCNGC 6 cut(s) 736, 739, 882, 894, 1083, 1158
BlsI GCNGC 6 cut(s) 737, 740, 883, 895, 1084, 1159
BmcAI AGTACT 1 cut(s) 50
Bme1390I CCNGG 4 cut(s) 44, 253, 944, 1255
Bme18I GGWCC 3 cut(s) 151, 276, 806
BmeT110I CYCGRG 1 cut(s) 1047
BmgT120I GGNCC 3 cut(s) 151, 276, 806
BmiI GGNNCC 6 cut(s) 153, 250, 277, 477, 807, 1310
BmrFI CCNGG 4 cut(s) 44, 253, 944, 1255
BmsI GCATC 6 cut(s) 105, 549, 905, 972, 1142, 1452
BpmI CTGGAG 2 cut(s) 65, 385
BpuEI CTTGAG 1 cut(s) 433
BpuMI CCSGG 2 cut(s) 944, 1255
Bsa29I ATCGAT 1 cut(s) 1136
BsaI GGTCTC 2 cut(s) 598, 1331
BsaJI CCNNGG 9 cut(s) 24, 71, 379, 773, 943, 1270, 1321, 1392, 1466
BsaXI ACNNNNNCTCC 3 cut(s) 34, 907, 937
Bse118I RCCGGY 1 cut(s) 692
Bse1I ACTGG 2 cut(s) 641, 1006
Bse21I CCTNAGG 1 cut(s) 1108
Bse3DI GCAATG 2 cut(s) 165, 846
BseBI CCWGG 2 cut(s) 44, 253
BseCI ATCGAT 1 cut(s) 1136
BseDI CCNNGG 9 cut(s) 24, 71, 379, 773, 943, 1270, 1321, 1392, 1466
BseGI GGATG 3 cut(s) 375, 1352, 1467
BseMI GCAATG 2 cut(s) 165, 846
BseNI ACTGG 2 cut(s) 641, 1006
BseRI GAGGAG 4 cut(s) 909, 912, 1374, 1509
BseSI GKGCMC 1 cut(s) 1001
BseXI GCAGC 5 cut(s) 722, 868, 905, 1069, 1169
BshFI GGCC 1 cut(s) 1182
BshNI GGYRCC 1 cut(s) 475
BshVI ATCGAT 1 cut(s) 1136
BsiHKAI GWGCWC 1 cut(s) 388
BsiHKCI CYCGRG 1 cut(s) 1047
BsiSI CCGG 3 cut(s) 693, 943, 1254
BslFI GGGAC 3 cut(s) 137, 605, 1268
BsmAI GTCTC 4 cut(s) 588, 598, 1331, 1491
BsmFI GGGAC 3 cut(s) 137, 605, 1268
BsmI GAATGC 2 cut(s) 1085, 1358
BsnI GGCC 1 cut(s) 1182
Bso31I GGTCTC 2 cut(s) 598, 1331
BsoBI CYCGRG 1 cut(s) 1047
Bsp1286I GDGCHC 3 cut(s) 388, 1001, 1260
Bsp143I GATC 3 cut(s) 28, 660, 669
Bsp19I CCATGG 3 cut(s) 24, 773, 1321
BspACI CCGC 6 cut(s) 263, 690, 739, 849, 1409, 1553
BspANI GGCC 1 cut(s) 1182
BspDI ATCGAT 1 cut(s) 1136
BspHI TCATGA 3 cut(s) 111, 346, 1089
BspLI GGNNCC 6 cut(s) 153, 250, 277, 477, 807, 1310
BspMAI CTGCAG 1 cut(s) 801
BspMI ACCTGC 1 cut(s) 815
BspPI GGATC 2 cut(s) 36, 677
BspT107I GGYRCC 1 cut(s) 475
BspTNI GGTCTC 2 cut(s) 598, 1331
BsrDI GCAATG 2 cut(s) 165, 846
BsrFI RCCGGY 1 cut(s) 692
BsrI ACTGG 2 cut(s) 641, 1006
BssAI RCCGGY 1 cut(s) 692
BssECI CCNNGG 9 cut(s) 24, 71, 379, 773, 943, 1270, 1321, 1392, 1466
BssMI GATC 3 cut(s) 28, 660, 669
BssSI CACGAG 2 cut(s) 186, 1184
BssT1I CCWWGG 6 cut(s) 24, 71, 379, 773, 1270, 1321
Bst2BI CACGAG 2 cut(s) 186, 1184
Bst2UI CCWGG 2 cut(s) 44, 253
Bst4CI ACNGT 5 cut(s) 636, 707, 1012, 1169, 1393
Bst6I CTCTTC 3 cut(s) 1181, 1271, 1506
BstC8I GCNNGC 3 cut(s) 261, 372, 956
BstDEI CTNAG 1 cut(s) 1108
BstDSI CCRYGG 5 cut(s) 24, 773, 1321, 1392, 1466
BstF5I GGATG 3 cut(s) 375, 1352, 1467
BstH2I RGCGCY 2 cut(s) 149, 242
BstHHI GCGC 2 cut(s) 148, 241
BstKTI GATC 3 cut(s) 31, 663, 672
BstMAI GTCTC 4 cut(s) 588, 598, 1331, 1491
BstMBI GATC 3 cut(s) 28, 660, 669
BstMWI GCNNNNNNNGC 4 cut(s) 698, 890, 960, 1130
BstNI CCWGG 2 cut(s) 44, 253
BstNSI RCATGY 1 cut(s) 602
BstSCI CCNGG 4 cut(s) 42, 251, 942, 1253
BstSFI CTRYAG 2 cut(s) 480, 797
BstSLI GKGCMC 1 cut(s) 1001
BstV1I GCAGC 5 cut(s) 722, 868, 905, 1069, 1169
BstX2I RGATCY 1 cut(s) 669
BstYI RGATCY 1 cut(s) 669
Bsu15I ATCGAT 1 cut(s) 1136
Bsu36I CCTNAGG 1 cut(s) 1108
BsuRI GGCC 1 cut(s) 1182
BsuTUI ATCGAT 1 cut(s) 1136
BtgI CCRYGG 5 cut(s) 24, 773, 1321, 1392, 1466
BtsCI GGATG 3 cut(s) 375, 1352, 1467
BtsI GCAGTG 1 cut(s) 806
BtsIMutI CAGTG 2 cut(s) 806, 999
BveI ACCTGC 1 cut(s) 815
Cac8I GCNNGC 3 cut(s) 261, 372, 956
CciI TCATGA 3 cut(s) 111, 346, 1089
CfoI GCGC 2 cut(s) 148, 241
Cfr10I RCCGGY 1 cut(s) 692
Cfr13I GGNCC 3 cut(s) 151, 276, 806
ClaI ATCGAT 1 cut(s) 1136
CsiI ACCWGGT 1 cut(s) 251
Csp6I GTAC 4 cut(s) 49, 123, 886, 993
CviQI GTAC 4 cut(s) 49, 123, 886, 993
DdeI CTNAG 1 cut(s) 1108
DpnI GATC 3 cut(s) 30, 662, 671
DpnII GATC 3 cut(s) 28, 660, 669
Eam1104I CTCTTC 3 cut(s) 1181, 1271, 1506
EarI CTCTTC 3 cut(s) 1181, 1271, 1506
Ecl136II GAGCTC 1 cut(s) 386
Eco130I CCWWGG 6 cut(s) 24, 71, 379, 773, 1270, 1321
Eco24I GRGCYC 2 cut(s) 388, 1260
Eco31I GGTCTC 2 cut(s) 598, 1331
Eco32I GATATC 1 cut(s) 1278
Eco47I GGWCC 3 cut(s) 151, 276, 806
Eco47III AGCGCT 1 cut(s) 240
Eco53kI GAGCTC 1 cut(s) 386
Eco57I CTGAAG 1 cut(s) 614
Eco81I CCTNAGG 1 cut(s) 1108
Eco88I CYCGRG 1 cut(s) 1047
EcoICRI GAGCTC 1 cut(s) 386
EcoO109I RGGNCCY 2 cut(s) 276, 806
EcoRI GAATTC 2 cut(s) 552, 1228
EcoRII CCWGG 2 cut(s) 42, 251
EcoRV GATATC 1 cut(s) 1278
EcoT14I CCWWGG 6 cut(s) 24, 71, 379, 773, 1270, 1321
EcoT22I ATGCAT 1 cut(s) 1360
EcoT38I GRGCYC 2 cut(s) 388, 1260
ErhI CCWWGG 6 cut(s) 24, 71, 379, 773, 1270, 1321
FaqI GGGAC 3 cut(s) 137, 605, 1268
FauI CCCGC 1 cut(s) 1560
Fnu4HI GCNGC 6 cut(s) 736, 739, 882, 894, 1083, 1158
FokI GGATG 3 cut(s) 362, 1359, 1474
FriOI GRGCYC 2 cut(s) 388, 1260
Fsp4HI GCNGC 6 cut(s) 736, 739, 882, 894, 1083, 1158
FspBI CTAG 1 cut(s) 92
GlaI GCGC 2 cut(s) 147, 240
GluI GCNGC 6 cut(s) 736, 739, 882, 894, 1083, 1158
GsuI CTGGAG 2 cut(s) 65, 385
HaeII RGCGCY 2 cut(s) 149, 242
HaeIII GGCC 1 cut(s) 1182
HapII CCGG 3 cut(s) 693, 943, 1254
HhaI GCGC 2 cut(s) 148, 241
Hin6I GCGC 2 cut(s) 146, 239
HinP1I GCGC 2 cut(s) 146, 239
HincII GTYRAC 1 cut(s) 876
HindII GTYRAC 1 cut(s) 876
HinfI GANTC 5 cut(s) 59, 136, 189, 872, 1545
HpaII CCGG 3 cut(s) 693, 943, 1254
HphI GGTGA 2 cut(s) 188, 1243
Hpy166II GTNNAC 2 cut(s) 464, 876
Hpy188I TCNGA 6 cut(s) 248, 558, 594, 1031, 1484, 1493
Hpy188III TCNNGA 8 cut(s) 112, 347, 658, 900, 1090, 1147, 1363, 1418
Hpy8I GTNNAC 2 cut(s) 464, 876
HpyAV CCTTC 4 cut(s) 150, 239, 1153, 1444
HpyCH4III ACNGT 5 cut(s) 636, 707, 1012, 1169, 1393
HpyCH4IV ACGT 1 cut(s) 1523
HpyCH4V TGCA 9 cut(s) 54, 158, 328, 799, 839, 1085, 1133, 1157, 1358
HpyF10VI GCNNNNNNNGC 4 cut(s) 698, 890, 960, 1130
HpyF3I CTNAG 1 cut(s) 1108
HpySE526I ACGT 1 cut(s) 1523
HspAI GCGC 2 cut(s) 146, 239
Kzo9I GATC 3 cut(s) 28, 660, 669
LmnI GCTCC 2 cut(s) 39, 383
Lsp1109I GCAGC 5 cut(s) 722, 868, 905, 1069, 1169
LweI GCATC 6 cut(s) 105, 549, 905, 972, 1142, 1452
MabI ACCWGGT 1 cut(s) 251
MaeI CTAG 1 cut(s) 92
MaeII ACGT 1 cut(s) 1523
MaeIII GTNAC 5 cut(s) 176, 422, 655, 707, 827
MalI GATC 3 cut(s) 30, 662, 671
MboI GATC 3 cut(s) 28, 660, 669
MboII GAAGA 7 cut(s) 1090, 1168, 1202, 1258, 1459, 1523, 1540
MfeI CAATTG 1 cut(s) 909
MflI RGATCY 1 cut(s) 669
MhlI GDGCHC 3 cut(s) 388, 1001, 1260
MlyI GAGTC 3 cut(s) 53, 198, 881
MmeI TCCRAC 5 cut(s) 87, 466, 1009, 1033, 1412
Mph1103I ATGCAT 1 cut(s) 1360
MroXI GAANNNNTTC 1 cut(s) 636
MseI TTAA 4 cut(s) 222, 686, 1476, 1533
MslI CAYNNNNRTG 4 cut(s) 113, 834, 1223, 1338
MspA1I CMGCKG 1 cut(s) 735
MspI CCGG 3 cut(s) 693, 943, 1254
MspR9I CCNGG 4 cut(s) 44, 253, 944, 1255
MunI CAATTG 1 cut(s) 909
Mva1269I GAATGC 2 cut(s) 1085, 1358
MvaI CCWGG 2 cut(s) 44, 253
MwoI GCNNNNNNNGC 4 cut(s) 698, 890, 960, 1130
NciI CCSGG 2 cut(s) 944, 1255
NcoI CCATGG 3 cut(s) 24, 773, 1321
NdeII GATC 3 cut(s) 28, 660, 669
NlaIV GGNNCC 6 cut(s) 153, 250, 277, 477, 807, 1310
NmuCI GTSAC 4 cut(s) 176, 655, 707, 827
NsiI ATGCAT 1 cut(s) 1360
NspI RCATGY 1 cut(s) 602
OliI CACNNNNGTG 1 cut(s) 834
PagI TCATGA 3 cut(s) 111, 346, 1089
PctI GAATGC 2 cut(s) 1085, 1358
PdmI GAANNNNTTC 1 cut(s) 636
PfeI GAWTC 2 cut(s) 136, 1545
PflMI CCANNNNNTGG 1 cut(s) 43
PkrI GCNGC 6 cut(s) 737, 740, 883, 895, 1084, 1159
PleI GAGTC 3 cut(s) 53, 197, 880
PpsI GAGTC 3 cut(s) 53, 197, 880
PpuMI RGGWCCY 2 cut(s) 276, 806
Psp124BI GAGCTC 1 cut(s) 388
Psp5II RGGWCCY 2 cut(s) 276, 806
Psp6I CCWGG 2 cut(s) 42, 251
PspGI CCWGG 2 cut(s) 42, 251
PspN4I GGNNCC 6 cut(s) 153, 250, 277, 477, 807, 1310
PspPI GGNCC 3 cut(s) 151, 276, 806
PspPPI RGGWCCY 2 cut(s) 276, 806
PstI CTGCAG 1 cut(s) 801
PsuI RGATCY 1 cut(s) 669
PvuII CAGCTG 1 cut(s) 735
RsaI GTAC 4 cut(s) 50, 124, 887, 994
RsaNI GTAC 4 cut(s) 49, 123, 886, 993
RseI CAYNNNNRTG 4 cut(s) 113, 834, 1223, 1338
SacI GAGCTC 1 cut(s) 388
SaqAI TTAA 4 cut(s) 222, 686, 1476, 1533
SatI GCNGC 6 cut(s) 736, 739, 882, 894, 1083, 1158
Sau3AI GATC 3 cut(s) 28, 660, 669
Sau96I GGNCC 3 cut(s) 151, 276, 806
ScaI AGTACT 1 cut(s) 50
SchI GAGTC 3 cut(s) 53, 198, 881
ScrFI CCNGG 4 cut(s) 44, 253, 944, 1255
SduI GDGCHC 3 cut(s) 388, 1001, 1260
SexAI ACCWGGT 1 cut(s) 251
SfaNI GCATC 6 cut(s) 105, 549, 905, 972, 1142, 1452
SfcI CTRYAG 2 cut(s) 480, 797
SgrAI CRCCGGYG 1 cut(s) 692
SinI GGWCC 3 cut(s) 151, 276, 806
SmiMI CAYNNNNRTG 4 cut(s) 113, 834, 1223, 1338
SmlI CTYRAG 1 cut(s) 448
SmoI CTYRAG 1 cut(s) 448
SsiI CCGC 6 cut(s) 263, 690, 739, 849, 1409, 1553
SspI AATATT 1 cut(s) 1304
SspMI CTAG 1 cut(s) 92
SstI GAGCTC 1 cut(s) 388
StyD4I CCNGG 4 cut(s) 42, 251, 942, 1253
StyI CCWWGG 6 cut(s) 24, 71, 379, 773, 1270, 1321
TaaI ACNGT 5 cut(s) 636, 707, 1012, 1169, 1393
TaiI ACGT 1 cut(s) 1526
TaqI TCGA 2 cut(s) 1136, 1445
TatI WGTACW 1 cut(s) 48
TauI GCSGC 1 cut(s) 741
TfiI GAWTC 2 cut(s) 136, 1545
Tru1I TTAA 4 cut(s) 222, 686, 1476, 1533
Tru9I TTAA 4 cut(s) 222, 686, 1476, 1533
TscAI CASTG 2 cut(s) 806, 1006
TseFI GTSAC 4 cut(s) 176, 655, 707, 827
TseI GCWGC 5 cut(s) 735, 881, 893, 1082, 1157
Tsp45I GTSAC 4 cut(s) 176, 655, 707, 827
TspDTI ATGAA 4 cut(s) 363, 1078, 1241, 1524
TspGWI ACGGA 2 cut(s) 346, 996
TspRI CASTG 2 cut(s) 806, 1006
Van91I CCANNNNNTGG 1 cut(s) 43
VpaK11BI GGWCC 3 cut(s) 151, 276, 806
XapI RAATTY 4 cut(s) 552, 1074, 1115, 1228
XceI RCATGY 1 cut(s) 602
XcmI CCANNNNNNNNNTGG 1 cut(s) 1340
XmnI GAANNNNTTC 1 cut(s) 636
XspI CTAG 1 cut(s) 92
ZrmI AGTACT 1 cut(s) 50
Zsp2I ATGCAT 1 cut(s) 1360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.