Rorug02G0467100

nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004561
Physical Location & Seq
Forward (+)
59843455 .. 59850354
6900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0467100.1

Sequence Viewer

Length: 3789 bp
ATGGATCGCAGCCTCTCTGAGCCTTTATTAGCCAAAACTAAATTAGACCAAGCCAGCTTCCTTAGCAAACTGACATTTTCTTGGGTCAATCCTCTCCTTAAGTTGGGTTCCTCGAGAGCATTAGATCTCGAAGACATCCCTTCTCTGGTTTCTGAAGACGAAGCAAATGTAGCATACCAGAAGTTTTCTGATGCATTGGAGTCCCTCTCGAGGGAGAAGAGCTCAAGTAGTACGTCGAGGAACTTGGTTCTCAGAGCCATGGCAAGAGTTTACATGGAGGAAAATGTGTTGATAATCATTTGTGCATTCCTCAAGACCATTGCTGTTGTAGTTTCTCCTCTTATACTCTATGCTTTTGTAAACTATTCAAATAGTGACAATGAAAGTCTCTCTGAAGGCCTCATTATAGTGGGGTATCTAGTTCTTACCAAAGTAGTTGAATCTTTGAGCCAGAGACACTGGTTTTTTGACTCAAGAAGGTCTGGCATGAGAATGAGGTCAGCCTTGATGGTTGCAGTCTATCAAAAGCAGCTGAAGCTTTCTAGTTTGGGAAGGAGAAGGCACTCAGCTGGAGAGATTGTGAATTACATAGCAGTGGACGCTTATCGAATGGGGGAGTTCCTATGGTGGTTTCATTCGACATGGAGCCTTTCGTTGCAACTATTGCTTTCCATTGTTGTACTTTACGTGGTCGTGGGTGTTGGTGCTCTACCTGGTTTAGTTCCTCTTATCATTTTTGGTCTTCTGAATGTGCCATTTGCTAAGGTACTTCAGAAGTGTCAGTCTGAGTTCATGGTAGCTCAGGATGAAAGGCTTAGAGCCACCTCTGAGATCCTAAACAGCATGAAAATCATTAAGTTACAATCATGGGAAGAGAAGTTCAAAAACTCGGTTGATGCCCTTCGCGAAAGAGAGTTCAAACCATTGGCTGAGGCACAATTTCAAAAGGCTTATGGAACTGTCATGTATTGGATGGCACCAACCATCATCTCTTCGGTTGTTTTTCTAGGATGCATTGTTTTCAACAGTGCTCCTCTAAATGCAAGTACTATATTCACAGTTCTAGCATCATTGAAAAGCATGGGGGAACCTGTCAGGTTTATACCGGAAGCACTTTCAGTTATGATACAAGTCAAGGTCTCGTTTGATCGGCTGAATTTTTTTCTGCTTGATAATGAGCTAAAGGATAGTGAAATAAGGAAAGTCCCATCACAGAATTCAGATGAGAGCTTAAAAATAGAAAGTGGCACGTTCAGCTGGAATCCTGAACTAACAACGATCCCAACTTTGAGAGATGTGAATTTGGAAGTAAAATGGGAGCAGAAAATTGCAGTTTGTGGACCAGTTGGTGCTGGAAAATCATCACTTTTGCATGCTTTACTTGGAGAGATGCCAAAAATGTCAGGAATGGTTGATATATTTGGAAGCATAGCCTATGTTTCTCAGACTTCTTGGATACAAAGTGGGACAGTTCGTGATAACATACTCTTCGGCAAGCCAATGGACAAGAACAGATATGAGAAGGCCATAAAAGCTTGTGCTTTGGATAAAGATATTACTAGCTTTGATCATGGTGATCTTACTGAAATAGGGCAGAGGGGACTTAACATGAGTGGAGGACAGAAGCAAAGGATTCAGCTGGCTCGAGCTGTCTATAATGATGCCGATATCTATCTCCTTGATGACCCCTTTAGTGCAGTGGATGCAAATACAGCTGCAATTCTATTTCATGATTGTGTCATGTCTGCTCTTGCAAAGAAAACTGTAATTCTGGTGACTCATCAAGTTGAATTTCTCTCACAAGTTGACAAGATTTTGGTAATTGAGGATGGACAAATTACTCAATCCGGAAGCTACGAGAGTCTCTTGACAGCAGGAACTGCATTTGCGAAGCTTGTGAATTCTCACAAAGATGCAGTAACAACATTGGGTCCTTCAAACTATCAAGTTCAAGAAGATTCTGAAAAGGAAGATATGATCCAGCCAGAGCAGTCTTATGGGAGTAACCGTAGTGTACCTGGGGTGCAGCTAACAGAAGAAGAAGAGAAGGTGATTGGTGATGTGGGGTTGAAGCCCTTTTGGGATTACATTTTTGTTTCTAAGGCAACGCTTCTTCTTTGCTTAGGCACAATAGCGCAGATTGGTTTTCTTGGTCTGCAGGTTGCTTCAACTTACTGGCTAGCTCTAGCCATTCAAATTCCTAAAATAACTAGCGGCATACTCATCGGAGTTTATACTGCAGTATCAACACTTAGTGTTGTCTTTGTATTTCTAAGGTCATACTTTGCAGCCCTTATGGGCTTGAAGGCGTCTAGAGCATTTTTCTGTAGTTTCATAGATGCTATCTTCAAGGCTCCCATGCTCTTCTTTGACTCAACTCCCGTCGGACGAATTTTGACACGAGCTTCATCAGATTTAAGTATATTGGATCTTGACATACCTTTCGCTACAATCTTTCTGGTGGCTTCTGGTACTGAACTGCTGACAGTGATTGGAATTATGGCTTCTGTCACATGGGAAGTTCTCATTGTAGCCATTCTCGCCATTATTGCTGCACAATATGTTCAGAGTTACTATCTAGCCTCTGCAAGGGAGCTGATAAGAATAAATGGAACAACAAAAGCTCCCGTAATAAATTATACAGCTGAGACATCACTTGGAGTGGTCTCTATAAGAGCTTTTACAATGGTTGACAGGTTCTTCCACAACTTCCTAAAGCTAGTTGACACAGATGCAAGTCTCTTCTTTCACTCCAATGCAACCATGGAGTGGTTAATTTTGAGGACAGAAGCACTTCAAAATGTGACCCTTTTCACCGCTGCTTTTCTCCTTATTCTACTTCCCAAGGGTTATGTTTCTCCAGGACTTGTGGGGCTTTCTCTTTCTTATGCTTTGACATTAACGGGGACGCAAATTTATCAGACTCGATGGTATTGCAATTTATCAAACTACATTATCTCAGTCGAAAGGATAAAACAATTCATGCAGATTTCACCTGAGCCTCTGGAAATTGTGGAGGACAATAGACCACCTTCTTCATGGCCTATGAAGGGTAGGATAGAGCTGTATTCTCTGAATATCAAATATCGTCAGAACGCTCCACTCGTTCTCAAGGGAATCACATGCACATTCAAAGAAGGGACTAGAGTAGGAGTTGTGGGAAGGACAGGAAGTGGCAAAACTACACTCATAAGTGCTTTGTTTCGTTTAGTAGAACCAAGCAGTGGTAAAATTATTATAGATGGACTTGACATCTGCTCTATTGGTCTAAAAGATTTGAGAATGAAGCTCAGCATCATCCCTCAAGAACCAACTCTTTTCAGGGGTAGCATTCGAACCAACTTGGATCCTCTAGGCCTATACTCTGACGATGAAATATGGAGGGCTCTAGAGAAGTGTCAGCTCAAGTTTACCGTCAGCAGCCTACCTAATCTTCTGGACTCGTCTGTGAGTGATGAAGGGGGAAATTGGAGTGCCGGGCAGCGCCAGCTGTTTTGCCTTGGAAGAGTGCTTCTCAAGAGGAACAGGATTCTAGTTCTAGATGAGGCTACTGCTTCCATTGATTCTGCAACAGATGCCATTCTACAAAGAATCATCAGGCTAGAATTTGCAGAATGCACAGTGATAACTGTGGCTCATAGAGTTCCAACAGTGATAGACAGCAACATGGTCATGGTCCTCTCCTATGGGAAGCTGGTGGAGTATGAAGAACCCTCAAAGCTCTTGGATACCAATTCCTATCTCTCCAAGCTTGTAGCTGAGTATTGGTCAAGCTGCAATAGAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0009506 GO:0030054 GO:0055044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Xan_ur_permease

Protein Analysis

1262

Amino Acids

140.26

Weight (kDa)

6.26

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 97 - 357 1.2e-27 ABC transporter transmembrane region
ABC_tran PF00005 430 - 564 2.9e-19 ABC transporter
ABC_membrane PF00664 737 - 970 7.3e-21 ABC transporter transmembrane region
ABC_tran PF00005 1037 - 1185 5.2e-26 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2149
AccB1I GGYRCC 1 cut(s) 976
AccB7I CCANNNNNTGG 2 cut(s) 2769, 3224
AccII CGCG 1 cut(s) 906
AccIII TCCGGA 1 cut(s) 1847
AciI CCGC 2 cut(s) 2214, 2817
AclWI GGATC 7 cut(s) 12, 826, 1273, 1972, 2436, 3341, 3354
AcsI RAATTY 9 cut(s) 1156, 1216, 1300, 1790, 1900, 2196, 2391, 2913, 3605
AcuI CTGAAG 4 cut(s) 174, 414, 554, 755
AcyI GRCGYC 1 cut(s) 2309
AdeI CACNNNGTG 1 cut(s) 2255
AfaI GTAC 6 cut(s) 232, 681, 768, 1048, 2016, 2473
AfiI CCNNNNNNNGG 8 cut(s) 103, 145, 210, 211, 2589, 2769, 3050, 3224
AflII CTTAAG 1 cut(s) 98
AjnI CCWGG 3 cut(s) 712, 2017, 2860
AloI GAACNNNNNNTCC 2 cut(s) 863, 895
Alw21I GWGCWC 3 cut(s) 224, 709, 1033
Alw26I GTCTC 7 cut(s) 392, 448, 1144, 1868, 2642, 2671, 2744
AlwI GGATC 7 cut(s) 12, 826, 1273, 1972, 2436, 3341, 3354
AlwNI CAGNNNCTG 1 cut(s) 1880
Ama87I CYCGRG 3 cut(s) 112, 208, 1644
Aor13HI TCCGGA 1 cut(s) 1847
AoxI GGCC 4 cut(s) 397, 1524, 3041, 3355
ApoI RAATTY 9 cut(s) 1156, 1216, 1300, 1790, 1900, 2196, 2391, 2913, 3605
ArsI GACNNNNNNTTYG 2 cut(s) 2426, 2458
Asp700I GAANNNNTTC 1 cut(s) 2793
AspLEI GCGC 2 cut(s) 2137, 3486
AspS9I GGNCC 3 cut(s) 1340, 1931, 3676
AsuC2I CCSGG 1 cut(s) 3478
AsuHPI GGTGA 6 cut(s) 1586, 1786, 2062, 2069, 2806, 2985
AsuII TTCGAA 1 cut(s) 3334
AsuNHI GCTAGC 1 cut(s) 2179
AvaI CYCGRG 3 cut(s) 112, 208, 1644
AvaII GGWCC 3 cut(s) 1340, 1931, 3676
BaeI ACNNNNGTAYC 2 cut(s) 2226, 2259
BamHI GGATCC 1 cut(s) 3346
BanI GGYRCC 1 cut(s) 976
BanII GRGCYC 2 cut(s) 224, 3388
BauI CACGAG 1 cut(s) 2400
BbsI GAAGAC 3 cut(s) 138, 162, 734
Bbv12I GWGCWC 3 cut(s) 224, 709, 1033
BbvCI CCTCAGC 1 cut(s) 930
BccI CCATC 7 cut(s) 502, 967, 992, 1216, 1823, 2922, 3236
BcgI CGANNNNNNTGC 4 cut(s) 2206, 2240, 2916, 2950
BciT130I CCWGG 3 cut(s) 714, 2019, 2862
BciVI GTATCC 2 cut(s) 1449, 3721
BclI TGATCA 1 cut(s) 1567
BcnI CCSGG 1 cut(s) 3478
BcoDI GTCTC 7 cut(s) 392, 448, 1144, 1868, 2642, 2671, 2744
BfmI CTRYAG 3 cut(s) 2156, 2238, 2326
BfoI RGCGCY 1 cut(s) 3487
BfrI CTTAAG 1 cut(s) 98
BfuAI ACCTGC 1 cut(s) 2149
BfuI GTATCC 2 cut(s) 1449, 3721
BglII AGATCT 1 cut(s) 124
BlpI GCTNAGC 1 cut(s) 3290
BmcAI AGTACT 1 cut(s) 1048
Bme1390I CCNGG 4 cut(s) 714, 2019, 2862, 3478
Bme18I GGWCC 3 cut(s) 1340, 1931, 3676
BmeT110I CYCGRG 3 cut(s) 112, 208, 1644
BmgT120I GGNCC 3 cut(s) 1340, 1931, 3676
BmiI GGNNCC 7 cut(s) 109, 647, 978, 1089, 1932, 2355, 3348
BmrFI CCNGG 4 cut(s) 714, 2019, 2862, 3478
BmtI GCTAGC 1 cut(s) 2183
BpiI GAAGAC 3 cut(s) 138, 162, 734
BplI GAGNNNNNCTC 6 cut(s) 191, 223, 206, 238, 3498, 3530
BpmI CTGGAG 2 cut(s) 591, 2844
Bpu10I CCTNAGC 6 cut(s) 62, 762, 801, 930, 2122, 2997
Bpu1102I GCTNAGC 1 cut(s) 3290
Bpu14I TTCGAA 1 cut(s) 3334
BpuEI CTTGAG 7 cut(s) 208, 296, 457, 3095, 3288, 3389, 3500
BpuMI CCSGG 1 cut(s) 3478
BsaAI YACGTR 1 cut(s) 688
BsaBI GATNNNNATC 1 cut(s) 1671
BsaHI GRCGYC 1 cut(s) 2309
BsaI GGTCTC 2 cut(s) 1144, 2671
BsaJI CCNNGG 5 cut(s) 258, 2018, 2763, 2844, 3499
BsaWI WCCGGW 2 cut(s) 1105, 1847
BsaXI ACNNNNNCTCC 6 cut(s) 564, 594, 1993, 2023, 2608, 2638
Bsc4I CCNNNNNNNGG 8 cut(s) 103, 145, 210, 211, 2589, 2769, 3050, 3224
Bse1I ACTGG 3 cut(s) 464, 1343, 2180
Bse3DI GCAATG 1 cut(s) 318
Bse8I GATNNNNATC 1 cut(s) 1671
BseAI TCCGGA 1 cut(s) 1847
BseBI CCWGG 3 cut(s) 714, 2019, 2862
BseDI CCNNGG 5 cut(s) 258, 2018, 2763, 2844, 3499
BseGI GGATG 7 cut(s) 135, 811, 978, 1016, 1708, 1834, 3297
BseJI GATNNNNATC 1 cut(s) 1671
BseLI CCNNNNNNNGG 8 cut(s) 103, 145, 210, 211, 2589, 2769, 3050, 3224
BseMI GCAATG 1 cut(s) 318
BseNI ACTGG 3 cut(s) 464, 1343, 2180
BseRI GAGGAG 2 cut(s) 327, 1023
BsgI GTGCAG 3 cut(s) 1716, 2045, 2538
Bsh1236I CGCG 1 cut(s) 906
BshFI GGCC 4 cut(s) 399, 1526, 3043, 3357
BshNI GGYRCC 1 cut(s) 976
BsiHKAI GWGCWC 3 cut(s) 224, 709, 1033
BsiHKCI CYCGRG 3 cut(s) 112, 208, 1644
BsiSI CCGG 3 cut(s) 1106, 1848, 3477
BslFI GGGAC 6 cut(s) 187, 1190, 1480, 1614, 2920, 3154
BslI CCNNNNNNNGG 8 cut(s) 103, 145, 210, 211, 2589, 2769, 3050, 3224
BsmAI GTCTC 7 cut(s) 392, 448, 1144, 1868, 2642, 2671, 2744
BsmFI GGGAC 6 cut(s) 187, 1190, 1480, 1614, 2920, 3154
BsmI GAATGC 3 cut(s) 305, 3330, 3620
BsnI GGCC 4 cut(s) 399, 1526, 3043, 3357
Bso31I GGTCTC 2 cut(s) 1144, 2671
BsoBI CYCGRG 3 cut(s) 112, 208, 1644
Bsp119I TTCGAA 1 cut(s) 3334
Bsp1286I GDGCHC 4 cut(s) 224, 709, 1033, 3388
Bsp13I TCCGGA 1 cut(s) 1847
Bsp1720I GCTNAGC 1 cut(s) 3290
Bsp19I CCATGG 2 cut(s) 258, 2763
Bsp68I TCGCGA 1 cut(s) 906
BspACI CCGC 2 cut(s) 2214, 2817
BspANI GGCC 4 cut(s) 399, 1526, 3043, 3357
BspEI TCCGGA 1 cut(s) 1847
BspFNI CGCG 1 cut(s) 906
BspHI TCATGA 1 cut(s) 1729
BspLI GGNNCC 7 cut(s) 109, 647, 978, 1089, 1932, 2355, 3348
BspMAI CTGCAG 2 cut(s) 2160, 2242
BspMI ACCTGC 1 cut(s) 2149
BspOI GCTAGC 1 cut(s) 2183
BspPI GGATC 7 cut(s) 12, 826, 1273, 1972, 2436, 3341, 3354
BspQI GCTCTTC 2 cut(s) 212, 2369
BspT104I TTCGAA 1 cut(s) 3334
BspT107I GGYRCC 1 cut(s) 976
BspTI CTTAAG 1 cut(s) 98
BspTNI GGTCTC 2 cut(s) 1144, 2671
BsrDI GCAATG 1 cut(s) 318
BsrI ACTGG 3 cut(s) 464, 1343, 2180
BssECI CCNNGG 5 cut(s) 258, 2018, 2763, 2844, 3499
BssNI GRCGYC 1 cut(s) 2309
BssSI CACGAG 1 cut(s) 2400
BssT1I CCWWGG 4 cut(s) 258, 2763, 2844, 3499
Bst2BI CACGAG 1 cut(s) 2400
Bst2UI CCWGG 3 cut(s) 714, 2019, 2862
Bst6I CTCTTC 8 cut(s) 212, 867, 997, 1493, 2037, 2369, 2747, 3499
BstACI GRCGYC 1 cut(s) 2309
BstAFI CTTAAG 1 cut(s) 98
BstAPI GCANNNNNTGC 4 cut(s) 664, 1703, 1880, 3575
BstBAI YACGTR 1 cut(s) 688
BstBI TTCGAA 1 cut(s) 3334
BstC8I GCNNGC 6 cut(s) 55, 1374, 1496, 1641, 2181, 3488
BstDSI CCRYGG 2 cut(s) 258, 2763
BstENI CCTNNNNNAGG 1 cut(s) 2587
BstF5I GGATG 7 cut(s) 135, 811, 978, 1016, 1708, 1834, 3297
BstFNI CGCG 1 cut(s) 906
BstH2I RGCGCY 1 cut(s) 3487
BstHHI GCGC 2 cut(s) 2137, 3486
BstMAI GTCTC 7 cut(s) 392, 448, 1144, 1868, 2642, 2671, 2744
BstNI CCWGG 3 cut(s) 714, 2019, 2862
BstNSI RCATGY 2 cut(s) 1376, 3126
BstSCI CCNGG 4 cut(s) 712, 2017, 2860, 3476
BstSFI CTRYAG 3 cut(s) 2156, 2238, 2326
BstUI CGCG 1 cut(s) 906
BstV2I GAAGAC 3 cut(s) 138, 162, 734
BstX2I RGATCY 4 cut(s) 124, 831, 2428, 3346
BstYI RGATCY 4 cut(s) 124, 831, 2428, 3346
BsuI GTATCC 2 cut(s) 1449, 3721
BsuRI GGCC 4 cut(s) 399, 1526, 3043, 3357
BtgI CCRYGG 2 cut(s) 258, 2763
BtsCI GGATG 7 cut(s) 135, 811, 978, 1016, 1708, 1834, 3297
BtsI GCAGTG 3 cut(s) 600, 1704, 3229
BtsIMutI CAGTG 8 cut(s) 457, 600, 1033, 1704, 2493, 3229, 3627, 3657
BtuMI TCGCGA 1 cut(s) 906
BveI ACCTGC 1 cut(s) 2149
Cac8I GCNNGC 6 cut(s) 55, 1374, 1496, 1641, 2181, 3488
CaiI CAGNNNCTG 1 cut(s) 1880
CciI TCATGA 1 cut(s) 1729
CfoI GCGC 2 cut(s) 2137, 3486
Cfr13I GGNCC 3 cut(s) 1340, 1931, 3676
CseI GACGC 3 cut(s) 608, 2298, 2917
CsiI ACCWGGT 1 cut(s) 712
Csp6I GTAC 6 cut(s) 231, 680, 767, 1047, 2015, 2472
CviQI GTAC 6 cut(s) 231, 680, 767, 1047, 2015, 2472
DraIII CACNNNGTG 1 cut(s) 2255
Eam1104I CTCTTC 8 cut(s) 212, 867, 997, 1493, 2037, 2369, 2747, 3499
EarI CTCTTC 8 cut(s) 212, 867, 997, 1493, 2037, 2369, 2747, 3499
Ecl136II GAGCTC 1 cut(s) 222
Eco130I CCWWGG 4 cut(s) 258, 2763, 2844, 3499
Eco147I AGGCCT 2 cut(s) 399, 3357
Eco24I GRGCYC 2 cut(s) 224, 3388
Eco31I GGTCTC 2 cut(s) 1144, 2671
Eco32I GATATC 1 cut(s) 1669
Eco47I GGWCC 3 cut(s) 1340, 1931, 3676
Eco53kI GAGCTC 1 cut(s) 222
Eco57I CTGAAG 4 cut(s) 174, 414, 554, 755
Eco88I CYCGRG 3 cut(s) 112, 208, 1644
EcoICRI GAGCTC 1 cut(s) 222
EcoNI CCTNNNNNAGG 1 cut(s) 2587
EcoO109I RGGNCCY 1 cut(s) 1931
EcoRI GAATTC 2 cut(s) 1216, 1900
EcoRII CCWGG 3 cut(s) 712, 2017, 2860
EcoRV GATATC 1 cut(s) 1669
EcoT14I CCWWGG 4 cut(s) 258, 2763, 2844, 3499
EcoT22I ATGCAT 2 cut(s) 196, 1016
EcoT38I GRGCYC 2 cut(s) 224, 3388
ErhI CCWWGG 4 cut(s) 258, 2763, 2844, 3499
FaqI GGGAC 6 cut(s) 187, 1190, 1480, 1614, 2920, 3154
FbaI TGATCA 1 cut(s) 1567
FokI GGATG 7 cut(s) 122, 818, 985, 1023, 1715, 1841, 3284
FriOI GRGCYC 2 cut(s) 224, 3388
GlaI GCGC 2 cut(s) 2136, 3485
GsuI CTGGAG 2 cut(s) 591, 2844
HaeII RGCGCY 1 cut(s) 3487
HaeIII GGCC 4 cut(s) 399, 1526, 3043, 3357
HapII CCGG 3 cut(s) 1106, 1848, 3477
HgaI GACGC 3 cut(s) 608, 2298, 2917
HhaI GCGC 2 cut(s) 2137, 3486
Hin1I GRCGYC 1 cut(s) 2309
Hin6I GCGC 2 cut(s) 2135, 3484
HinP1I GCGC 2 cut(s) 2135, 3484
HincII GTYRAC 3 cut(s) 1807, 2692, 2725
HindII GTYRAC 3 cut(s) 1807, 2692, 2725
HindIII AAGCTT 4 cut(s) 536, 1533, 1892, 3749
HpaII CCGG 3 cut(s) 1106, 1848, 3477
HphI GGTGA 6 cut(s) 1586, 1786, 2062, 2069, 2806, 2985
Hpy166II GTNNAC 9 cut(s) 271, 361, 598, 1340, 1807, 2015, 2692, 2725, 3411
Hpy8I GTNNAC 9 cut(s) 271, 361, 598, 1340, 1807, 2015, 2692, 2725, 3411
Hpy99I CGWCG 2 cut(s) 238, 2387
HpyCH4IV ACGT 3 cut(s) 233, 687, 1250
HpySE526I ACGT 3 cut(s) 233, 687, 1250
Hsp92I GRCGYC 1 cut(s) 2309
HspAI GCGC 2 cut(s) 2135, 3484
Kpn2I TCCGGA 1 cut(s) 1847
Ksp22I TGATCA 1 cut(s) 1567
LguI GCTCTTC 2 cut(s) 212, 2369
LmnI GCTCC 7 cut(s) 645, 1036, 1318, 2359, 2593, 2629, 3103
MabI ACCWGGT 1 cut(s) 712
MaeII ACGT 3 cut(s) 233, 687, 1250
MaeIII GTNAC 8 cut(s) 374, 858, 1774, 1918, 2003, 2509, 2570, 2803
MflI RGATCY 4 cut(s) 124, 831, 2428, 3346
MhlI GDGCHC 4 cut(s) 224, 709, 1033, 3388
MlyI GAGTC 7 cut(s) 209, 464, 1771, 1870, 2366, 2917, 3434
MmeI TCCRAC 2 cut(s) 2365, 3671
Mph1103I ATGCAT 2 cut(s) 196, 1016
MroI TCCGGA 1 cut(s) 1847
MroXI GAANNNNTTC 1 cut(s) 2793
MseI TTAA 7 cut(s) 99, 855, 1232, 1605, 2417, 2774, 2900
MslI CAYNNNNRTG 7 cut(s) 407, 491, 593, 1734, 1911, 2754, 3671
MspA1I CMGCKG 8 cut(s) 532, 569, 1257, 1639, 1715, 2645, 2819, 3490
MspCI CTTAAG 1 cut(s) 98
MspI CCGG 3 cut(s) 1106, 1848, 3477
MspR9I CCNGG 4 cut(s) 714, 2019, 2862, 3478
Mva1269I GAATGC 3 cut(s) 305, 3330, 3620
MvaI CCWGG 3 cut(s) 714, 2019, 2862
MvnI CGCG 1 cut(s) 906
NciI CCSGG 1 cut(s) 3478
NcoI CCATGG 2 cut(s) 258, 2763
NheI GCTAGC 1 cut(s) 2179
NlaIV GGNNCC 7 cut(s) 109, 647, 978, 1089, 1932, 2355, 3348
NmuCI GTSAC 4 cut(s) 374, 1774, 2509, 2803
NruI TCGCGA 1 cut(s) 906
NsiI ATGCAT 2 cut(s) 196, 1016
NspI RCATGY 2 cut(s) 1376, 3126
NspV TTCGAA 1 cut(s) 3334
PaeI GCATGC 1 cut(s) 1376
PaeR7I CTCGAG 3 cut(s) 112, 208, 1644
PagI TCATGA 1 cut(s) 1729
PceI AGGCCT 2 cut(s) 399, 3357
PciSI GCTCTTC 2 cut(s) 212, 2369
PcsI WCGNNNNNNNCGW 1 cut(s) 3102
PctI GAATGC 3 cut(s) 305, 3330, 3620
PdmI GAANNNNTTC 1 cut(s) 2793
PfeI GAWTC 8 cut(s) 440, 1261, 1633, 1958, 3117, 3529, 3563, 3591
PflMI CCANNNNNTGG 2 cut(s) 2769, 3224
PfoI TCCNGGA 1 cut(s) 2860
PleI GAGTC 7 cut(s) 208, 464, 1771, 1869, 2366, 2917, 3434
PpsI GAGTC 7 cut(s) 208, 464, 1771, 1869, 2366, 2917, 3434
Ppu21I YACGTR 1 cut(s) 688
PpuMI RGGWCCY 1 cut(s) 1931
Psp124BI GAGCTC 1 cut(s) 224
Psp5II RGGWCCY 1 cut(s) 1931
Psp6I CCWGG 3 cut(s) 712, 2017, 2860
PspGI CCWGG 3 cut(s) 712, 2017, 2860
PspN4I GGNNCC 7 cut(s) 109, 647, 978, 1089, 1932, 2355, 3348
PspPI GGNCC 3 cut(s) 1340, 1931, 3676
PspPPI RGGWCCY 1 cut(s) 1931
PspXI VCTCGAGB 1 cut(s) 1644
PstI CTGCAG 2 cut(s) 2160, 2242
PstNI CAGNNNCTG 1 cut(s) 1880
PsuI RGATCY 4 cut(s) 124, 831, 2428, 3346
PvuII CAGCTG 7 cut(s) 532, 569, 1257, 1639, 1715, 2645, 3490
RruI TCGCGA 1 cut(s) 906
RsaI GTAC 6 cut(s) 232, 681, 768, 1048, 2016, 2473
RsaNI GTAC 6 cut(s) 231, 680, 767, 1047, 2015, 2472
RseI CAYNNNNRTG 7 cut(s) 407, 491, 593, 1734, 1911, 2754, 3671
SacI GAGCTC 1 cut(s) 224
SapI GCTCTTC 2 cut(s) 212, 2369
SaqAI TTAA 7 cut(s) 99, 855, 1232, 1605, 2417, 2774, 2900
Sau96I GGNCC 3 cut(s) 1340, 1931, 3676
ScaI AGTACT 1 cut(s) 1048
SchI GAGTC 7 cut(s) 209, 464, 1771, 1870, 2366, 2917, 3434
ScrFI CCNGG 4 cut(s) 714, 2019, 2862, 3478
SduI GDGCHC 4 cut(s) 224, 709, 1033, 3388
SexAI ACCWGGT 1 cut(s) 712
SfcI CTRYAG 3 cut(s) 2156, 2238, 2326
Sfr274I CTCGAG 3 cut(s) 112, 208, 1644
SfuI TTCGAA 1 cut(s) 3334
SinI GGWCC 3 cut(s) 1340, 1931, 3676
SlaI CTCGAG 3 cut(s) 112, 208, 1644
SmiMI CAYNNNNRTG 7 cut(s) 407, 491, 593, 1734, 1911, 2754, 3671
SphI GCATGC 1 cut(s) 1376
SseBI AGGCCT 2 cut(s) 399, 3357
SsiI CCGC 2 cut(s) 2214, 2817
SstI GAGCTC 1 cut(s) 224
StuI AGGCCT 2 cut(s) 399, 3357
StyD4I CCNGG 4 cut(s) 712, 2017, 2860, 3476
StyI CCWWGG 4 cut(s) 258, 2763, 2844, 3499
TaiI ACGT 3 cut(s) 236, 690, 1253
TatI WGTACW 2 cut(s) 679, 1046
TauI GCSGC 1 cut(s) 2217
TfiI GAWTC 8 cut(s) 440, 1261, 1633, 1958, 3117, 3529, 3563, 3591
Tru1I TTAA 7 cut(s) 99, 855, 1232, 1605, 2417, 2774, 2900
Tru9I TTAA 7 cut(s) 99, 855, 1232, 1605, 2417, 2774, 2900
TscAI CASTG 8 cut(s) 464, 600, 1033, 1704, 2493, 3229, 3627, 3657
TseFI GTSAC 4 cut(s) 374, 1774, 2509, 2803
Tsp45I GTSAC 4 cut(s) 374, 1774, 2509, 2803
TspRI CASTG 8 cut(s) 464, 600, 1033, 1704, 2493, 3229, 3627, 3657
Van91I CCANNNNNTGG 2 cut(s) 2769, 3224
Vha464I CTTAAG 1 cut(s) 98
VpaK11BI GGWCC 3 cut(s) 1340, 1931, 3676
XagI CCTNNNNNAGG 1 cut(s) 2587
XapI RAATTY 9 cut(s) 1156, 1216, 1300, 1790, 1900, 2196, 2391, 2913, 3605
XbaI TCTAGA 3 cut(s) 2312, 3388, 3538
XceI RCATGY 2 cut(s) 1376, 3126
XcmI CCANNNNNNNNNTGG 2 cut(s) 2761, 3036
XhoI CTCGAG 3 cut(s) 112, 208, 1644
XmnI GAANNNNTTC 1 cut(s) 2793
ZrmI AGTACT 1 cut(s) 1048
Zsp2I ATGCAT 2 cut(s) 196, 1016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.