Rorug02G0466900

nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004561
Physical Location & Seq
Reverse (-)
59820718 .. 59822197
1480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0466900.1

Sequence Viewer

Length: 1191 bp
ATGGCGGATTCTTCATCAAAAGGACGCACCCTGTTCTCTCCTTACAAGATGGGCAGGTTCAATCTCTCTCACAGGATAGTGCTTGCGCCACTGACGAGGTGTCGAGCACATAACGAAATTCCGCAGCCAGCGATGGTCAAGTATTATTCTCAGAGGTCGACCCCCGGTGGCTTGTTAATCAGCGAAGGCACTTCGCCCTCCGACACAAGCGCTGGGTATCCACGTGTTCCTGGGATTTACACGGATGAACAGGTCGAGGCATGGAAAAAGGTTGTCGACGCCGTTCACGCCAAAGGTGCCATTTTTTTCTGTCAACTTTGGCATGTAGGTCGTGCTTCTCATCAAGTTTACCAACCTGGCGGGGGCTCGCCGATTTCTTCAACCAACAATCCCATTTCAAATGGATGGAAAGTTCCGATGCCAGATGGATCTCAAGAGACTAATTACCCACAGCCTCGACGCCTCGAAACCCACGAAATACCTCAGATTGTTGAGCAGTATCGCCGCGCTGCCTTAAATGCAGTTCTAGCAGGTTTTGATGGAATTGAGATTCATGCGGCACACGGCTACCTCATTGATCAATTCTTGAAAGATGGAATCAACGATCGAACTGATGAGTATGGTGGGTCGCTTGCAAACCGTTGCAAATTCTTGATTCAAATTGTTCAAGCAGTAGTTGAAGCAATTGGTGCTGACAGAGTTGCCGTCAGAATCTCACCAGCTATTGATTTTCATGATGCTACGGACTCCGACCCGATTGGCCTAGGCCTTGCAGTGATTGGCAGGCTCAATGAGCTTCAATACAACGTAGGTTCAAAGCTCGCTTATCTCCATGTGACTCAGCCTCGTTTAGCTACTCAAATGAACTTAGGCAGGGCCGAGATTGATCAAGACAGAGAAGCTCAATTCATGCGGACTTTGCGACAAGCTTATCAAGGTACATTCATGGCTAGTGGAGGGTTTACGAGAGAACTAGGAATGGAAACCGTGGCTTCCGGTGATGCTGATTTAGTGTCGTTTGGCCGAAATTTTATCTCAAACCCTGACTTGGTGTTGAGGTTTAAGTTGAATGCTCCTTTGAACAAGTATATTAGGGAGACTTTCTACACCGATGATCCTGTAGTTGGCTACACAGACTACCCTTTTCTGGAAACAGAAAGTGTGAAAGAGGATGACCTCTCCCCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0009506 GO:0030054 GO:0055044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Xan_ur_permease

Protein Analysis

396

Amino Acids

43.88

Weight (kDa)

5.95

Isoelectric Point (pI)

36.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_FMN PF00724 11 - 360 1.1e-86 NADH:flavin oxidoreductase / NADH oxidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 45, 521
AccB1I GGYRCC 1 cut(s) 296
AccI GTMKAC 2 cut(s) 158, 276
AccII CGCG 1 cut(s) 507
AciI CCGC 6 cut(s) 5, 122, 360, 505, 557, 913
AclWI GGATC 2 cut(s) 436, 1109
AcoI YGGCCR 1 cut(s) 1021
AcsI RAATTY 3 cut(s) 117, 647, 1027
AcvI CACGTG 1 cut(s) 224
AcyI GRCGYC 2 cut(s) 279, 460
AfaI GTAC 1 cut(s) 940
AfeI AGCGCT 1 cut(s) 211
AfiI CCNNNNNNNGG 4 cut(s) 362, 1048, 1124, 1147
AflIII ACRYGT 1 cut(s) 223
AhdI GACNNNNNGTC 1 cut(s) 99
AjnI CCWGG 2 cut(s) 229, 355
AloI GAACNNNNNNTCC 2 cut(s) 396, 428
AluBI AGCT 6 cut(s) 722, 796, 820, 854, 902, 929
AluI AGCT 6 cut(s) 722, 796, 820, 854, 902, 929
Alw21I GWGCWC 1 cut(s) 109
Alw26I GTCTC 2 cut(s) 431, 1091
AlwI GGATC 2 cut(s) 436, 1109
Aor51HI AGCGCT 1 cut(s) 211
AoxI GGCC 4 cut(s) 760, 766, 876, 1021
ApeKI GCWGC 2 cut(s) 124, 509
ApoI RAATTY 3 cut(s) 117, 647, 1027
AspA2I CCTAGG 1 cut(s) 763
AspLEI GCGC 3 cut(s) 88, 212, 509
AspS9I GGNCC 1 cut(s) 876
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 2 cut(s) 708, 1010
AvrII CCTAGG 1 cut(s) 763
BanI GGYRCC 1 cut(s) 296
BanII GRGCYC 1 cut(s) 368
BbrPI CACGTG 1 cut(s) 224
Bbv12I GWGCWC 1 cut(s) 109
BbvI GCAGC 2 cut(s) 136, 496
BccI CCATC 6 cut(s) 43, 127, 399, 419, 533, 587
BceAI ACGGC 3 cut(s) 266, 580, 689
BcgI CGANNNNNNTGC 2 cut(s) 311, 345
BciT130I CCWGG 2 cut(s) 231, 357
BciVI GTATCC 1 cut(s) 228
BclI TGATCA 2 cut(s) 577, 886
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 2 cut(s) 431, 1091
BfaI CTAG 4 cut(s) 527, 764, 951, 974
BfmI CTRYAG 1 cut(s) 1119
BfoI RGCGCY 1 cut(s) 213
BfuAI ACCTGC 2 cut(s) 45, 521
BfuI GTATCC 1 cut(s) 228
BisI GCNGC 4 cut(s) 125, 505, 510, 558
BlnI CCTAGG 1 cut(s) 763
BlsI GCNGC 4 cut(s) 126, 506, 511, 559
Bme1390I CCNGG 3 cut(s) 165, 231, 357
BmeRI GACNNNNNGTC 1 cut(s) 99
BmgT120I GGNCC 1 cut(s) 876
BmiI GGNNCC 1 cut(s) 298
BmrFI CCNGG 3 cut(s) 165, 231, 357
BmsI GCATC 3 cut(s) 408, 727, 991
BpuEI CTTGAG 1 cut(s) 417
BpuMI CCSGG 1 cut(s) 165
BsaAI YACGTR 1 cut(s) 224
BsaHI GRCGYC 2 cut(s) 279, 460
BsaJI CCNNGG 4 cut(s) 163, 230, 763, 987
BsaWI WCCGGW 1 cut(s) 995
Bsc4I CCNNNNNNNGG 4 cut(s) 362, 1048, 1124, 1147
BseBI CCWGG 2 cut(s) 231, 357
BseDI CCNNGG 4 cut(s) 163, 230, 763, 987
BseGI GGATG 3 cut(s) 250, 410, 1177
BseLI CCNNNNNNNGG 4 cut(s) 362, 1048, 1124, 1147
BseMII CTCAG 3 cut(s) 164, 497, 854
BseXI GCAGC 2 cut(s) 136, 496
BseYI CCCAGC 1 cut(s) 212
Bsh1236I CGCG 1 cut(s) 507
Bsh1285I CGRYCG 1 cut(s) 607
BshFI GGCC 4 cut(s) 762, 768, 878, 1023
BshNI GGYRCC 1 cut(s) 296
BsiEI CGRYCG 1 cut(s) 607
BsiHKAI GWGCWC 1 cut(s) 109
BsiSI CCGG 2 cut(s) 165, 996
BslI CCNNNNNNNGG 4 cut(s) 362, 1048, 1124, 1147
BsmAI GTCTC 2 cut(s) 431, 1091
BsmI GAATGC 1 cut(s) 1075
BsnI GGCC 4 cut(s) 762, 768, 878, 1023
Bsp1286I GDGCHC 2 cut(s) 109, 368
Bsp143I GATC 5 cut(s) 428, 577, 604, 886, 1114
BspACI CCGC 6 cut(s) 5, 122, 360, 505, 557, 913
BspANI GGCC 4 cut(s) 762, 768, 878, 1023
BspCNI CTCAG 3 cut(s) 163, 496, 853
BspFNI CGCG 1 cut(s) 507
BspHI TCATGA 1 cut(s) 733
BspLI GGNNCC 1 cut(s) 298
BspMI ACCTGC 2 cut(s) 45, 521
BspPI GGATC 2 cut(s) 436, 1109
BspT107I GGYRCC 1 cut(s) 296
BssECI CCNNGG 4 cut(s) 163, 230, 763, 987
BssMI GATC 5 cut(s) 428, 577, 604, 886, 1114
BssNI GRCGYC 2 cut(s) 279, 460
BssT1I CCWWGG 1 cut(s) 763
Bst2UI CCWGG 2 cut(s) 231, 357
Bst4CI ACNGT 2 cut(s) 641, 988
BstACI GRCGYC 2 cut(s) 279, 460
BstAPI GCANNNNNTGC 1 cut(s) 689
BstBAI YACGTR 1 cut(s) 224
BstC8I GCNNGC 6 cut(s) 84, 129, 368, 633, 785, 822
BstDEI CTNAG 4 cut(s) 150, 483, 840, 868
BstDSI CCRYGG 1 cut(s) 987
BstF5I GGATG 3 cut(s) 250, 410, 1177
BstFNI CGCG 1 cut(s) 507
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 3 cut(s) 88, 212, 509
BstKTI GATC 5 cut(s) 431, 580, 607, 889, 1117
BstMAI GTCTC 2 cut(s) 431, 1091
BstMBI GATC 5 cut(s) 428, 577, 604, 886, 1114
BstMCI CGRYCG 1 cut(s) 607
BstMWI GCNNNNNNNGC 7 cut(s) 287, 296, 518, 527, 689, 793, 919
BstNI CCWGG 2 cut(s) 231, 357
BstNSI RCATGY 1 cut(s) 326
BstSCI CCNGG 3 cut(s) 163, 229, 355
BstSFI CTRYAG 1 cut(s) 1119
BstUI CGCG 1 cut(s) 507
BstV1I GCAGC 2 cut(s) 136, 496
BstX2I RGATCY 1 cut(s) 428
BstYI RGATCY 1 cut(s) 428
BsuI GTATCC 1 cut(s) 228
BsuRI GGCC 4 cut(s) 762, 768, 878, 1023
BtgI CCRYGG 1 cut(s) 987
BtgZI GCGATG 1 cut(s) 146
BtsCI GGATG 3 cut(s) 250, 410, 1177
BtsI GCAGTG 1 cut(s) 780
BtsIMutI CAGTG 2 cut(s) 89, 780
BveI ACCTGC 2 cut(s) 45, 521
Cac8I GCNNGC 6 cut(s) 84, 129, 368, 633, 785, 822
CciI TCATGA 1 cut(s) 733
CfoI GCGC 3 cut(s) 88, 212, 509
Cfr13I GGNCC 1 cut(s) 876
CseI GACGC 3 cut(s) 33, 287, 468
Csp6I GTAC 1 cut(s) 939
CviAII CATG 7 cut(s) 261, 323, 554, 734, 833, 910, 946
CviQI GTAC 1 cut(s) 939
DdeI CTNAG 4 cut(s) 150, 483, 840, 868
DpnI GATC 5 cut(s) 430, 579, 606, 888, 1116
DpnII GATC 5 cut(s) 428, 577, 604, 886, 1114
DriI GACNNNNNGTC 1 cut(s) 99
EaeI YGGCCR 1 cut(s) 1021
Eam1105I GACNNNNNGTC 1 cut(s) 99
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 763
Eco147I AGGCCT 1 cut(s) 768
Eco24I GRGCYC 1 cut(s) 368
Eco47III AGCGCT 1 cut(s) 211
Eco72I CACGTG 1 cut(s) 224
EcoRII CCWGG 2 cut(s) 229, 355
EcoT14I CCWWGG 1 cut(s) 763
EcoT38I GRGCYC 1 cut(s) 368
ErhI CCWWGG 1 cut(s) 763
FaeI CATG 7 cut(s) 264, 326, 557, 737, 836, 913, 949
FatI CATG 7 cut(s) 260, 322, 553, 733, 832, 909, 945
FauI CCCGC 1 cut(s) 353
FbaI TGATCA 2 cut(s) 577, 886
FblI GTMKAC 2 cut(s) 158, 276
Fnu4HI GCNGC 4 cut(s) 125, 505, 510, 558
FokI GGATG 3 cut(s) 257, 417, 1184
FriOI GRGCYC 1 cut(s) 368
Fsp4HI GCNGC 4 cut(s) 125, 505, 510, 558
FspBI CTAG 4 cut(s) 527, 764, 951, 974
GlaI GCGC 3 cut(s) 87, 211, 508
GluI GCNGC 4 cut(s) 125, 505, 510, 558
GsaI CCCAGC 1 cut(s) 216
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 4 cut(s) 762, 768, 878, 1023
HapII CCGG 2 cut(s) 165, 996
HgaI GACGC 3 cut(s) 33, 287, 468
HhaI GCGC 3 cut(s) 88, 212, 509
Hin1I GRCGYC 2 cut(s) 279, 460
Hin1II CATG 7 cut(s) 264, 326, 557, 737, 836, 913, 949
Hin6I GCGC 3 cut(s) 86, 210, 507
HinP1I GCGC 3 cut(s) 86, 210, 507
HincII GTYRAC 3 cut(s) 159, 277, 314
HindII GTYRAC 3 cut(s) 159, 277, 314
HindIII AAGCTT 1 cut(s) 927
HinfI GANTC 7 cut(s) 8, 550, 597, 655, 711, 746, 838
HpaII CCGG 2 cut(s) 165, 996
HphI GGTGA 2 cut(s) 708, 1010
Hpy166II GTNNAC 6 cut(s) 159, 277, 286, 314, 349, 963
Hpy188I TCNGA 6 cut(s) 153, 202, 417, 486, 710, 751
Hpy188III TCNNGA 6 cut(s) 434, 586, 652, 734, 890, 1148
Hpy8I GTNNAC 6 cut(s) 159, 277, 286, 314, 349, 963
Hpy99I CGWCG 2 cut(s) 281, 462
HpyAV CCTTC 1 cut(s) 179
HpyCH4III ACNGT 2 cut(s) 641, 988
HpyCH4IV ACGT 2 cut(s) 223, 807
HpyCH4V TGCA 4 cut(s) 521, 635, 645, 773
HpyF10VI GCNNNNNNNGC 7 cut(s) 287, 296, 518, 527, 689, 793, 919
HpyF3I CTNAG 4 cut(s) 150, 483, 840, 868
HpySE526I ACGT 2 cut(s) 223, 807
Hsp92I GRCGYC 2 cut(s) 279, 460
Hsp92II CATG 7 cut(s) 264, 326, 557, 737, 836, 913, 949
HspAI GCGC 3 cut(s) 86, 210, 507
Ksp22I TGATCA 2 cut(s) 577, 886
Kzo9I GATC 5 cut(s) 428, 577, 604, 886, 1114
LmnI GCTCC 1 cut(s) 1078
Lsp1109I GCAGC 2 cut(s) 136, 496
LweI GCATC 3 cut(s) 408, 727, 991
MaeI CTAG 4 cut(s) 527, 764, 951, 974
MaeII ACGT 2 cut(s) 223, 807
MaeIII GTNAC 1 cut(s) 835
MalI GATC 5 cut(s) 430, 579, 606, 888, 1116
MboI GATC 5 cut(s) 428, 577, 604, 886, 1114
MboII GAAGA 2 cut(s) 3, 369
MfeI CAATTG 1 cut(s) 684
MflI RGATCY 1 cut(s) 428
MhlI GDGCHC 2 cut(s) 109, 368
MluCI AATT 9 cut(s) 117, 442, 543, 581, 647, 660, 684, 905, 1027
MlyI GAGTC 2 cut(s) 740, 832
MmeI TCCRAC 2 cut(s) 225, 774
MseI TTAA 3 cut(s) 176, 515, 1062
MspI CCGG 2 cut(s) 165, 996
MspR9I CCNGG 3 cut(s) 165, 231, 357
MunI CAATTG 1 cut(s) 684
Mva1269I GAATGC 1 cut(s) 1075
MvaI CCWGG 2 cut(s) 231, 357
MvnI CGCG 1 cut(s) 507
MwoI GCNNNNNNNGC 7 cut(s) 287, 296, 518, 527, 689, 793, 919
NciI CCSGG 1 cut(s) 165
NdeII GATC 5 cut(s) 428, 577, 604, 886, 1114
NlaIII CATG 7 cut(s) 264, 326, 557, 737, 836, 913, 949
NlaIV GGNNCC 1 cut(s) 298
NmeAIII GCCGAG 1 cut(s) 904
NmuCI GTSAC 1 cut(s) 835
NspI RCATGY 1 cut(s) 326
PagI TCATGA 1 cut(s) 733
PceI AGGCCT 1 cut(s) 768
PcsI WCGNNNNNNNCGW 1 cut(s) 471
PctI GAATGC 1 cut(s) 1075
PfeI GAWTC 5 cut(s) 8, 550, 597, 655, 711
PkrI GCNGC 4 cut(s) 126, 506, 511, 559
Ple19I CGATCG 1 cut(s) 607
PleI GAGTC 2 cut(s) 740, 832
PmaCI CACGTG 1 cut(s) 224
PmlI CACGTG 1 cut(s) 224
PpsI GAGTC 2 cut(s) 740, 832
Ppu21I YACGTR 1 cut(s) 224
Psp6I CCWGG 2 cut(s) 229, 355
PspCI CACGTG 1 cut(s) 224
PspFI CCCAGC 1 cut(s) 212
PspGI CCWGG 2 cut(s) 229, 355
PspN4I GGNNCC 1 cut(s) 298
PspPI GGNCC 1 cut(s) 876
PsuI RGATCY 1 cut(s) 428
PvuI CGATCG 1 cut(s) 607
RsaI GTAC 1 cut(s) 940
RsaNI GTAC 1 cut(s) 939
SalI GTCGAC 2 cut(s) 157, 275
SaqAI TTAA 3 cut(s) 176, 515, 1062
SatI GCNGC 4 cut(s) 125, 505, 510, 558
Sau3AI GATC 5 cut(s) 428, 577, 604, 886, 1114
Sau96I GGNCC 1 cut(s) 876
SchI GAGTC 2 cut(s) 740, 832
ScrFI CCNGG 3 cut(s) 165, 231, 357
SduI GDGCHC 2 cut(s) 109, 368
SfaNI GCATC 3 cut(s) 408, 727, 991
SfcI CTRYAG 1 cut(s) 1119
SmlI CTYRAG 1 cut(s) 432
SmoI CTYRAG 1 cut(s) 432
Sse9I AATT 9 cut(s) 117, 442, 543, 581, 647, 660, 684, 905, 1027
SseBI AGGCCT 1 cut(s) 768
SsiI CCGC 6 cut(s) 5, 122, 360, 505, 557, 913
SspMI CTAG 4 cut(s) 527, 764, 951, 974
StuI AGGCCT 1 cut(s) 768
StyD4I CCNGG 3 cut(s) 163, 229, 355
StyI CCWWGG 1 cut(s) 763
TaaI ACNGT 2 cut(s) 641, 988
TaiI ACGT 2 cut(s) 226, 810
TaqI TCGA 7 cut(s) 103, 158, 255, 276, 457, 465, 607
TasI AATT 9 cut(s) 117, 442, 543, 581, 647, 660, 684, 905, 1027
TauI GCSGC 2 cut(s) 507, 560
TfiI GAWTC 5 cut(s) 8, 550, 597, 655, 711
Tru1I TTAA 3 cut(s) 176, 515, 1062
Tru9I TTAA 3 cut(s) 176, 515, 1062
TscAI CASTG 2 cut(s) 96, 780
TseFI GTSAC 1 cut(s) 835
TseI GCWGC 2 cut(s) 124, 509
Tsp45I GTSAC 1 cut(s) 835
TspDTI ATGAA 6 cut(s) 261, 542, 722, 878, 898, 934
TspGWI ACGGA 2 cut(s) 257, 758
TspRI CASTG 2 cut(s) 96, 780
XapI RAATTY 3 cut(s) 117, 647, 1027
XceI RCATGY 1 cut(s) 326
XmaJI CCTAGG 1 cut(s) 763
XmiI GTMKAC 2 cut(s) 158, 276
XspI CTAG 4 cut(s) 527, 764, 951, 974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.