MD09G1119500.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014360
Physical Location & Seq
Reverse (-)
9222421 .. 9223662
1242 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1119500.v1.1.491

Sequence Viewer

Length: 1242 bp
ATGGACTCAGACTCTGAATTGCAGCAGAGCAAAAGGAGGAGGAGCCACCAGAGAGAAGATCAATCAGTAACTGGCTTGGAAAATTTACCACATGAAATCATCACTGAGGTAACTTCAAGGCTGCCCTTTTCATCTCTGGTAAAATTCAGGTATGTATGCAGAGCGTGGAAAAATTTAGCACAAGACCAACATCTTCGAATTTTCGATGCTTTGCATAACTCTAACACAGCTGATCACAACAATCTGTGCCTGATTTTTCACTGTGATTACCCCATCAGAAACCATATTTACTTTGTAGATTACCCTTTTCACCATGGTGAAAAGGAGACAATGCTAGTGAAAAAAATTCACCCTCCTTTCTGTGGTTCGATGCCTGAGTTTGATGTGGTAGGATCATGTGATGGTTTGCTGTGCTTATCTGATTCGTTGTACAATGATGCAATATGCATATATAATCCGTTCACTAGGGATCATAGAGAGCTGCCTAAATCAGTTCGATTTCCTAATCAAGAAGTGATTTACGGATTCGGGTTTCATCCGATTACTAAGGAGTACAAGGTAGTGAAGATAGTGTACTATAGTAGACGAGAATCATGGAGCCGGTTTCGAGTGTATCGGCCACAATCAGAAGTTCAAGTTTTCACTCTTGGAACTTCTGATTGGAGAAGTTTAGGAGGAACATCTCACTACCTTCATCACTGGCCAGCTCAGGTCTTGGTCAATGGAAGGCTTCATTGGGTTACTTGGAGAAAGGGTTACCACCCTGGTCGAAAGCTCATTTCTTTTGACTTAGGAGACGAGCAGTTCAGGGAGGTACCGAAACCGGAAGTTGACGGATTGAGCAGGTGGAATTATCATGTGCTGGTTGTAAGAGGTTGTCTTGCTGCATTTTTTTACTGCAGTTATGGGAAATTGGAGATGTGGGTTATGGAGGAGTACGGTGTGAAGGAATCATGGGTTAAAGAGTTGACCATTGGAAGTTATGTGCCAAAGACATTGAAACAAGATGTTGATAGATCATGGAAGATTTCAAAGATTGTTAAAAGAGGAAGACATGTTAGGGTTTTGTGTGTTTTGGGGAGTGGCCAGATCTTGTTGGAGTACAAAAGCAGAGCTCTAGTTGTTTATGAACCAAGTAGTGGAAAGTTTAAGGATCTTTTGTTTCAGGGGATGCCTAAGTGGTTTCAAACCGTTGTTCACGAGGGCACTCTCAATCCGATACATACTCTTGTTAACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0008150 GO:0008284 GO:0009506 GO:0030054 GO:0042127 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0055044 GO:0065007
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1 FBA_3

Protein Analysis

414

Amino Acids

48.42

Weight (kDa)

8.99

Isoelectric Point (pI)

45.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 26 - 66 4.9e-09 F-box domain
F-box-like PF12937 26 - 65 2.8e-08 F-box-like
FBA_3 PF08268 98 - 393 1e-30 F-box associated beta propeller domain
Beta-prop_KIB1-4 PF03478 123 - 320 1.3e-06 KIB1-4 beta-propeller
FBA_1 PF07734 124 - 334 9e-23 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 834
Acc36I ACCTGC 1 cut(s) 834
Acc65I GGTACC 1 cut(s) 814
AccB1I GGYRCC 1 cut(s) 814
AccB7I CCANNNNNTGG 1 cut(s) 1139
AccI GTMKAC 1 cut(s) 583
AclWI GGATC 3 cut(s) 400, 477, 1161
AcoI YGGCCR 3 cut(s) 617, 701, 1084
AcsI RAATTY 5 cut(s) 82, 143, 172, 198, 345
AfaI GTAC 6 cut(s) 431, 554, 575, 816, 938, 1103
AfiI CCNNNNNNNGG 2 cut(s) 362, 1139
AflIII ACRYGT 2 cut(s) 1054, 1236
AgsI TTSAA 5 cut(s) 117, 635, 1000, 1032, 1187
AjnI CCWGG 1 cut(s) 763
AjuI GAANNNNNNNTTGG 4 cut(s) 643, 675, 718, 750
AleI CACNNNNGTG 1 cut(s) 315
AluBI AGCT 5 cut(s) 230, 481, 707, 775, 1115
AluI AGCT 5 cut(s) 230, 481, 707, 775, 1115
Alw21I GWGCWC 1 cut(s) 1117
Alw26I GTCTC 2 cut(s) 320, 789
AlwI GGATC 3 cut(s) 400, 477, 1161
AlwNI CAGNNNCTG 2 cut(s) 14, 71
AoxI GGCC 3 cut(s) 617, 701, 1084
ApeKI GCWGC 4 cut(s) 22, 121, 481, 884
ApoI RAATTY 5 cut(s) 82, 143, 172, 198, 345
Asp718I GGTACC 1 cut(s) 814
AsuHPI GGTGA 3 cut(s) 302, 329, 341
AsuII TTCGAA 1 cut(s) 196
BaeGI GKGCMC 1 cut(s) 1208
BalI TGGCCA 2 cut(s) 703, 1086
BanI GGYRCC 1 cut(s) 814
BanII GRGCYC 1 cut(s) 1117
BarI GAAGNNNNNNTAC 2 cut(s) 557, 589
BauI CACGAG 1 cut(s) 1199
BbsI GAAGAC 1 cut(s) 1057
Bbv12I GWGCWC 1 cut(s) 1117
BbvI GCAGC 4 cut(s) 34, 108, 468, 871
BccI CCATC 2 cut(s) 281, 395
BciT130I CCWGG 1 cut(s) 765
BclI TGATCA 1 cut(s) 232
BcoDI GTCTC 2 cut(s) 320, 789
BfaI CTAG 3 cut(s) 335, 465, 1118
BfmI CTRYAG 2 cut(s) 577, 898
BfuAI ACCTGC 1 cut(s) 834
BglII AGATCT 1 cut(s) 1089
BisI GCNGC 4 cut(s) 23, 122, 482, 885
BlsI GCNGC 4 cut(s) 24, 123, 483, 886
Bme1390I CCNGG 1 cut(s) 765
BmiI GGNNCC 3 cut(s) 44, 599, 816
BmrFI CCNGG 1 cut(s) 765
BmsI GCATC 4 cut(s) 196, 360, 427, 1161
BpiI GAAGAC 1 cut(s) 1057
Bpu10I CCTNAGC 1 cut(s) 708
Bpu14I TTCGAA 1 cut(s) 196
BsaJI CCNNGG 2 cut(s) 313, 763
BsaWI WCCGGW 1 cut(s) 823
BsaXI ACNNNNNCTCC 2 cut(s) 739, 769
Bsc4I CCNNNNNNNGG 2 cut(s) 362, 1139
Bse118I RCCGGY 1 cut(s) 600
Bse1I ACTGG 2 cut(s) 76, 704
BseBI CCWGG 1 cut(s) 765
BseDI CCNNGG 2 cut(s) 313, 763
BseGI GGATG 2 cut(s) 535, 1176
BseLI CCNNNNNNNGG 2 cut(s) 362, 1139
BseMII CTCAG 4 cut(s) 21, 96, 366, 722
BseNI ACTGG 2 cut(s) 76, 704
BseRI GAGGAG 3 cut(s) 52, 55, 947
BseSI GKGCMC 1 cut(s) 1208
BseXI GCAGC 4 cut(s) 34, 108, 468, 871
BshFI GGCC 3 cut(s) 619, 703, 1086
BshNI GGYRCC 1 cut(s) 814
BsiHKAI GWGCWC 1 cut(s) 1117
BsiSI CCGG 2 cut(s) 601, 824
BslI CCNNNNNNNGG 2 cut(s) 362, 1139
BsmAI GTCTC 2 cut(s) 320, 789
BsmBI CGTCTC 1 cut(s) 789
BsnI GGCC 3 cut(s) 619, 703, 1086
Bsp119I TTCGAA 1 cut(s) 196
Bsp1286I GDGCHC 2 cut(s) 1117, 1208
Bsp1407I TGTACA 1 cut(s) 429
Bsp143I GATC 7 cut(s) 58, 232, 392, 469, 1016, 1089, 1153
Bsp19I CCATGG 1 cut(s) 313
BspANI GGCC 3 cut(s) 619, 703, 1086
BspCNI CTCAG 4 cut(s) 20, 97, 367, 721
BspLI GGNNCC 3 cut(s) 44, 599, 816
BspMAI CTGCAG 1 cut(s) 902
BspMI ACCTGC 1 cut(s) 834
BspPI GGATC 3 cut(s) 400, 477, 1161
BspT104I TTCGAA 1 cut(s) 196
BspT107I GGYRCC 1 cut(s) 814
BsrFI RCCGGY 1 cut(s) 600
BsrGI TGTACA 1 cut(s) 429
BsrI ACTGG 2 cut(s) 76, 704
BssAI RCCGGY 1 cut(s) 600
BssECI CCNNGG 2 cut(s) 313, 763
BssMI GATC 7 cut(s) 58, 232, 392, 469, 1016, 1089, 1153
BssSI CACGAG 1 cut(s) 1199
BssT1I CCWWGG 1 cut(s) 313
Bst2BI CACGAG 1 cut(s) 1199
Bst2UI CCWGG 1 cut(s) 765
Bst4CI ACNGT 3 cut(s) 263, 941, 1192
BstAUI TGTACA 1 cut(s) 429
BstBI TTCGAA 1 cut(s) 196
BstC8I GCNNGC 1 cut(s) 705
BstDEI CTNAG 7 cut(s) 7, 105, 375, 546, 708, 790, 1176
BstDSI CCRYGG 1 cut(s) 313
BstEII GGTNACC 1 cut(s) 755
BstF5I GGATG 2 cut(s) 535, 1176
BstKTI GATC 7 cut(s) 61, 235, 395, 472, 1019, 1092, 1156
BstMAI GTCTC 2 cut(s) 320, 789
BstMBI GATC 7 cut(s) 58, 232, 392, 469, 1016, 1089, 1153
BstNI CCWGG 1 cut(s) 765
BstNSI RCATGY 2 cut(s) 1058, 1240
BstPI GGTNACC 1 cut(s) 755
BstSCI CCNGG 1 cut(s) 763
BstSFI CTRYAG 2 cut(s) 577, 898
BstSLI GKGCMC 1 cut(s) 1208
BstV1I GCAGC 4 cut(s) 34, 108, 468, 871
BstV2I GAAGAC 1 cut(s) 1057
BstX2I RGATCY 2 cut(s) 1089, 1153
BstYI RGATCY 2 cut(s) 1089, 1153
BsuRI GGCC 3 cut(s) 619, 703, 1086
BtgI CCRYGG 1 cut(s) 313
BtsCI GGATG 2 cut(s) 535, 1176
BtsIMutI CAGTG 3 cut(s) 102, 259, 697
BveI ACCTGC 1 cut(s) 834
Cac8I GCNNGC 1 cut(s) 705
CaiI CAGNNNCTG 2 cut(s) 14, 71
Cfr10I RCCGGY 1 cut(s) 600
Csp6I GTAC 6 cut(s) 430, 553, 574, 815, 937, 1102
CviAII CATG 9 cut(s) 92, 314, 396, 594, 857, 954, 1020, 1055, 1237
CviQI GTAC 6 cut(s) 430, 553, 574, 815, 937, 1102
DdeI CTNAG 7 cut(s) 7, 105, 375, 546, 708, 790, 1176
DpnI GATC 7 cut(s) 60, 234, 394, 471, 1018, 1091, 1155
DpnII GATC 7 cut(s) 58, 232, 392, 469, 1016, 1089, 1153
EaeI YGGCCR 3 cut(s) 617, 701, 1084
Ecl136II GAGCTC 1 cut(s) 1115
Eco130I CCWWGG 1 cut(s) 313
Eco24I GRGCYC 1 cut(s) 1117
Eco53kI GAGCTC 1 cut(s) 1115
Eco91I GGTNACC 1 cut(s) 755
EcoICRI GAGCTC 1 cut(s) 1115
EcoO65I GGTNACC 1 cut(s) 755
EcoRII CCWGG 1 cut(s) 763
EcoT14I CCWWGG 1 cut(s) 313
EcoT22I ATGCAT 1 cut(s) 449
EcoT38I GRGCYC 1 cut(s) 1117
ErhI CCWWGG 1 cut(s) 313
Esp3I CGTCTC 1 cut(s) 789
FaeI CATG 9 cut(s) 95, 317, 399, 597, 860, 957, 1023, 1058, 1240
FatI CATG 9 cut(s) 91, 313, 395, 593, 856, 953, 1019, 1054, 1236
FbaI TGATCA 1 cut(s) 232
FblI GTMKAC 1 cut(s) 583
Fnu4HI GCNGC 4 cut(s) 23, 122, 482, 885
FokI GGATG 2 cut(s) 522, 1183
FriOI GRGCYC 1 cut(s) 1117
Fsp4HI GCNGC 4 cut(s) 23, 122, 482, 885
FspBI CTAG 3 cut(s) 335, 465, 1118
GluI GCNGC 4 cut(s) 23, 122, 482, 885
HaeIII GGCC 3 cut(s) 619, 703, 1086
HapII CCGG 2 cut(s) 601, 824
Hin1II CATG 9 cut(s) 95, 317, 399, 597, 860, 957, 1023, 1058, 1240
HincII GTYRAC 3 cut(s) 832, 969, 1234
HindII GTYRAC 3 cut(s) 832, 969, 1234
HinfI GANTC 6 cut(s) 5, 11, 422, 525, 590, 950
HpaI GTTAAC 1 cut(s) 1234
HpaII CCGG 2 cut(s) 601, 824
HphI GGTGA 3 cut(s) 302, 329, 341
Hpy166II GTNNAC 7 cut(s) 462, 574, 584, 832, 969, 1198, 1234
Hpy188I TCNGA 8 cut(s) 10, 16, 278, 421, 540, 628, 658, 1218
Hpy188III TCNNGA 2 cut(s) 509, 1199
Hpy8I GTNNAC 7 cut(s) 462, 574, 584, 832, 969, 1198, 1234
HpyAV CCTTC 3 cut(s) 701, 720, 940
HpyCH4III ACNGT 3 cut(s) 263, 941, 1192
HpyCH4V TGCA 7 cut(s) 22, 159, 214, 440, 447, 887, 900
HpyF3I CTNAG 7 cut(s) 7, 105, 375, 546, 708, 790, 1176
Hsp92II CATG 9 cut(s) 95, 317, 399, 597, 860, 957, 1023, 1058, 1240
KpnI GGTACC 1 cut(s) 818
Ksp22I TGATCA 1 cut(s) 232
KspAI GTTAAC 1 cut(s) 1234
Kzo9I GATC 7 cut(s) 58, 232, 392, 469, 1016, 1089, 1153
LmnI GCTCC 2 cut(s) 42, 597
Lsp1109I GCAGC 4 cut(s) 34, 108, 468, 871
LweI GCATC 4 cut(s) 196, 360, 427, 1161
MaeI CTAG 3 cut(s) 335, 465, 1118
MaeIII GTNAC 4 cut(s) 67, 109, 739, 755
MalI GATC 7 cut(s) 60, 234, 394, 471, 1018, 1091, 1155
MboI GATC 7 cut(s) 58, 232, 392, 469, 1016, 1089, 1153
MboII GAAGA 5 cut(s) 68, 185, 577, 1036, 1062
MflI RGATCY 2 cut(s) 1089, 1153
MhlI GDGCHC 2 cut(s) 1117, 1208
MlsI TGGCCA 2 cut(s) 703, 1086
MluCI AATT 8 cut(s) 17, 82, 143, 172, 198, 345, 850, 911
MluNI TGGCCA 2 cut(s) 703, 1086
MlyI GAGTC 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 1077
Mox20I TGGCCA 2 cut(s) 703, 1086
Mph1103I ATGCAT 1 cut(s) 449
MscI TGGCCA 2 cut(s) 703, 1086
MseI TTAA 4 cut(s) 960, 1041, 1149, 1233
MslI CAYNNNNRTG 1 cut(s) 315
Msp20I TGGCCA 2 cut(s) 703, 1086
MspA1I CMGCKG 1 cut(s) 230
MspI CCGG 2 cut(s) 601, 824
MspR9I CCNGG 1 cut(s) 765
MvaI CCWGG 1 cut(s) 765
NcoI CCATGG 1 cut(s) 313
NdeII GATC 7 cut(s) 58, 232, 392, 469, 1016, 1089, 1153
NlaIII CATG 9 cut(s) 95, 317, 399, 597, 860, 957, 1023, 1058, 1240
NlaIV GGNNCC 3 cut(s) 44, 599, 816
NsiI ATGCAT 1 cut(s) 449
NspI RCATGY 2 cut(s) 1058, 1240
NspV TTCGAA 1 cut(s) 196
OliI CACNNNNGTG 1 cut(s) 315
PaqCI CACCTGC 1 cut(s) 834
PciI ACATGT 2 cut(s) 1054, 1236
PfeI GAWTC 4 cut(s) 422, 525, 590, 950
PflMI CCANNNNNTGG 1 cut(s) 1139
PkrI GCNGC 4 cut(s) 24, 123, 483, 886
PleI GAGTC 1 cut(s) 5
PpsI GAGTC 1 cut(s) 5
PscI ACATGT 2 cut(s) 1054, 1236
Psp124BI GAGCTC 1 cut(s) 1117
Psp6I CCWGG 1 cut(s) 763
PspEI GGTNACC 1 cut(s) 755
PspGI CCWGG 1 cut(s) 763
PspN4I GGNNCC 3 cut(s) 44, 599, 816
PstI CTGCAG 1 cut(s) 902
PstNI CAGNNNCTG 2 cut(s) 14, 71
PsuI RGATCY 2 cut(s) 1089, 1153
PvuII CAGCTG 1 cut(s) 230
RsaI GTAC 6 cut(s) 431, 554, 575, 816, 938, 1103
RsaNI GTAC 6 cut(s) 430, 553, 574, 815, 937, 1102
RseI CAYNNNNRTG 1 cut(s) 315
SacI GAGCTC 1 cut(s) 1117
SaqAI TTAA 4 cut(s) 960, 1041, 1149, 1233
SatI GCNGC 4 cut(s) 23, 122, 482, 885
Sau3AI GATC 7 cut(s) 58, 232, 392, 469, 1016, 1089, 1153
SchI GAGTC 1 cut(s) 5
ScrFI CCNGG 1 cut(s) 765
SduI GDGCHC 2 cut(s) 1117, 1208
SfaNI GCATC 4 cut(s) 196, 360, 427, 1161
SfcI CTRYAG 2 cut(s) 577, 898
SfuI TTCGAA 1 cut(s) 196
SmiMI CAYNNNNRTG 1 cut(s) 315
Sse9I AATT 8 cut(s) 17, 82, 143, 172, 198, 345, 850, 911
SspMI CTAG 3 cut(s) 335, 465, 1118
SstI GAGCTC 1 cut(s) 1117
StyD4I CCNGG 1 cut(s) 763
StyI CCWWGG 1 cut(s) 313
TaaI ACNGT 3 cut(s) 263, 941, 1192
TaqI TCGA 6 cut(s) 196, 204, 368, 496, 607, 769
TasI AATT 8 cut(s) 17, 82, 143, 172, 198, 345, 850, 911
TatI WGTACW 4 cut(s) 429, 552, 573, 1101
TfiI GAWTC 4 cut(s) 422, 525, 590, 950
Tru1I TTAA 4 cut(s) 960, 1041, 1149, 1233
Tru9I TTAA 4 cut(s) 960, 1041, 1149, 1233
TscAI CASTG 3 cut(s) 109, 266, 704
TseI GCWGC 4 cut(s) 22, 121, 481, 884
TspDTI ATGAA 6 cut(s) 108, 120, 524, 683, 722, 1143
TspGWI ACGGA 3 cut(s) 447, 537, 849
TspRI CASTG 3 cut(s) 109, 266, 704
Van91I CCANNNNNTGG 1 cut(s) 1139
XapI RAATTY 5 cut(s) 82, 143, 172, 198, 345
XceI RCATGY 2 cut(s) 1058, 1240
XmiI GTMKAC 1 cut(s) 583
XspI CTAG 3 cut(s) 335, 465, 1118
Zsp2I ATGCAT 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.