Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002434

Physical Location & Seq

Genomic Coordinates Reverse (-)

9996855 .. 10002224

Gene Structure 5370 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1129700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2193 bp

ATGGAGGATTCTGAGAAAATTGTGTTTAACTCACCGGTGCAAGACAGTATGGTTGCCGATGATCACTTGTCAGTTCTGAAAGGCTATGGAACATACGGCATTGTTGGTGCTGTTGTTGTTGCCGTACTTATACCTGCATTTCTGGCCACTGTGTTTGGGGGGAAGAAAAAGGGGAAGCAGAGAGGAGTTCCAGTAGAAGTTGGTGGTGAGGCAGGTTATGCAATGCGCAATGCTCGGGAAACTGAGTTGATTGAAGTTCCTCAGAAAGGAGCCACAACCATGGCAGCCCTTTTTGAACAATCTTGTAAAAAGTACTCACATAACCGATTTCTTGGAACAAGGAAGTTTATTGGAAGGGAATTTGTTACAGCTAGCGATGGAAGGAAGTTTGAGAAGGTTCACATGGGAGAGTATGAATGGCAATCTTATGGAGAAGTGTTTGATCGTGCTTGCAACTTTGCATCTGGGCTTATTAAGTTAGGTCATAATGTGGATACCCGTGCAGCAATTTTCTCAGAAACCCGCGCAGAGTGGCTTATTGCCTTTCAGGGATTCTTCCGGCAGAATATTACCGTTGTCACTATTTATGCTTCACTAGGAGAGGATGCGTTAATTCACTCCCTTAATGAGACCCAAGTTTCGACATTGATCTGTGATTCCAAGCAGTTGAAAAAGTTGGCTGCTATAACCTCAAGCCTAAACACTATTCAGAATGTTATTTATTTTGAAGACGAGGGAAGTTCAGATACTAGTGTAACTGGAAGTATGGGCGATTTGAAAGTTGCATCCTTTTCTGAAGTTGAGAAACTTGGAAAAGAGAGTCCTGTTCCTCCAAGTTTACCGTCCAAGAATGGTATTGCTGTTATTATGTATACAAGTGGAAGTACAGGTCTACCGAAGGGAGTCATGATAACTCATGGCAACATTTTAGCCACTACTGCAGCGGTTATGACGGTGGTTCCGAGTCTGGGTAGCGCTGATGTATACCTGGCATACTTGCCCCTGGCTCATGTTTTCGAACTGGCAGCTGAGTCTGTGATGCTGGCTGCAGGTTGTTCAATTGGTTATGGTTCAGCATTGACTTTAACAGACACTTCAAATAAAGTTAAAAAAGGAACCAAGGGAGATGCAACTATGTTACAGCCAACCATCATGACAGCGGTTCCTGCTATTTTAGATCGTGTTCGAGATGGAGTTCTGAAAAAGGTTAATGAGAAAGGGGGCTTTGCTAAGAATCTTTTTAATCTTGGGTTTAGACGGCGACTGGCAGCTGTAGAAGGAAGCTGGTTTGGGGCTTGGGGTTTGGAAAGTATGTTGTGGGATGTCGTTGTCTTCAAGAAAATACGCTCATTACTTGGAGGACGCATTCGTTTTATGCTCTGTGGTGGTGCTCCTTTATCTGGTGATTCACAGCGATTCATCAATATTTGCATGGGGGCTCCTATTGGGCAAGGATATGGTTTGACAGAAACATTTGCTGGAGCTACATTTTCTGAGTGGGATGACACAACTGTGGGCCGTGTTGGTCCTCCCCTTCCTTGTGGGTACATTAAGCTTGTTTCCTGGAAAGAAGGAGGATACTTGCCATCAGACAAGCCTATGCCTCGTGGGGAGATTGTAGTCGGGGGATACAGTGTAACTGCTGGTTACTTTAAGAACCAAGAAAAGACTGACGAGGTGTTTAAGGTTGATGAGAAGGGTATGCGCTGGTTTTATACTGGAGACATTGGAATGTTTCATCCTGATGGATGCCTTGAAATTATTGATAGGAAGAAGGATATCGTTAAACTCCAGCATGGAGAGTACATTTCCCTTGGCAAGGTTGAAGCGGCGTTAGCATCAAGTAATTATGTGGACAACGTCATGGCGTATGCTGATCCCTCGCACAACTACTGCGTAGCTCTAATTGTCCCTGCAAGTCAGGCCCTCGAGAAGTGGGCCCATGAAGCCGAAGTCAAGTTCAATGATTTTGCCGAGCTGTGTGAAAAGGCTGAAACAGTCAGTGAGGTTCAGCAGTCCCTCTCCAAGGTAGCAAAAGCGTCGAAACTGGACAAGTTCGAACTTCCTGCCAAGATTAAGTTGGTGACTGAGCCTTGGACACCGGAGTCCGGATTGGTTACCGCTGCTATGAAGATTAAGAGAGAACAGATCAAGGCCAAGTTCAAAAATGAGCTAAACAAGTTGTATGCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001676 GO:0003674 GO:0003824 GO:0004467 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006082 GO:0006629 GO:0006631 GO:0008150 GO:0008152 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0012505 GO:0015645 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0019752 GO:0031967 GO:0031975 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00061 ko00071 ko01100 ko01212 ko02024 ko03320 ko04146 ko04216 ko04714 ko04920 map00061 map00071 map01100 map01212 map02024 map03320 map04146 map04216 map04714 map04920

Pfam Domains

Protein Families

AMP-binding

Protein Analysis

731

Amino Acids

79.44

Weight (kDa)

7.52

Isoelectric Point (pI)

28.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	131 - 551	1e-82	AMP-binding enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002434)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04350 AT2G04350
fragaria_vesca	FvH4_6g40470 FvH4_6g40470 FvH4_6g40470 FvH4_6g40470 FvH4_6g40470 FvH4_6g40470 FvH4_6g40470 FvH4_6g40470
malus_domestica	MD09G1129700.v1.1 MD17G1118500.v1.1
prunus_persica	Prupe.3G195600_v2.0.a1 Prupe.3G195600_v2.0.a1 Prupe.3G195600_v2.0.a1 Prupe.3G195600_v2.0.a1
pyrus_communis	pycom09g05270 pycom17g10890
rosa_chinensis	RchiOBHm_Chr2g0155701 RchiOBHm_Chr2g0155721
rosa_laevigata	RLG00000020855 RLG00000020857 RLG00000020858
rosa_multiflora	Rmu_co8272995.1_g000001 Rmu_co8427559.1_g000001 Rmu_sc0002712.1_g000001 Rmu_sc0011953.1_g000003 Rmu_sc0011953.1_g000015
rosa_roxburghii	Rroxscaffold_2G00093540
rosa_rugosa	Rorug02G0449500
rosa_samantha	Rh2AG515400 Rh2AG515500 Rh2BG526400 Rh2BG526500 Rh2CG500300 Rh2CG500500 Rh2DG535300 Rh2DG535500
rosa_wichuraiana	Rw2G042360 Rw2G042370 Rw2G042390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2104
Acc16I	TGCGCA	1 cut(s)	227
Acc36I	ACCTGC	3 cut(s)	142, 203, 1040
AccI	GTMKAC	3 cut(s)	872, 892, 984
AccII	CGCG	1 cut(s)	525
AccIII	TCCGGA	1 cut(s)	2108
AciI	CCGC	5 cut(s)	523, 944, 1160, 1829, 2121
AclWI	GGATC	1 cut(s)	1871
AcoI	YGGCCR	1 cut(s)	144
AcsI	RAATTY	1 cut(s)	359
AcuI	CTGAAG	1 cut(s)	816
AfaI	GTAC	5 cut(s)	126, 314, 886, 1547, 1805
AfeI	AGCGCT	1 cut(s)	976
AfiI	CCNNNNNNNGG	6 cut(s)	266, 968, 1400, 1819, 2026, 2108
AgeI	ACCGGT	1 cut(s)	34
AhlI	ACTAGT	1 cut(s)	749
AjnI	CCWGG	3 cut(s)	987, 1002, 1562
AleI	CACNNNNGTG	1 cut(s)	1511
AluBI	AGCT	9 cut(s)	371, 1028, 1271, 1284, 1484, 1555, 1901, 1978, 2173
AluI	AGCT	9 cut(s)	371, 1028, 1271, 1284, 1484, 1555, 1901, 1978, 2173
Alw21I	GWGCWC	1 cut(s)	1393
Alw26I	GTCTC	2 cut(s)	623, 1716
AlwI	GGATC	1 cut(s)	1871
Ama87I	CYCGRG	2 cut(s)	234, 1928
Aor13HI	TCCGGA	1 cut(s)	2108
Aor51HI	AGCGCT	1 cut(s)	976
AoxI	GGCC	5 cut(s)	144, 1516, 1923, 1938, 2154
ApaI	GGGCCC	1 cut(s)	1942
ApeKI	GCWGC	8 cut(s)	284, 503, 680, 941, 1025, 1046, 1268, 2123
ApoI	RAATTY	1 cut(s)	359
ArsI	GACNNNNNNTTYG	2 cut(s)	622, 654
AsiGI	ACCGGT	1 cut(s)	34
AspLEI	GCGC	4 cut(s)	228, 527, 977, 1707
AspS9I	GGNCC	5 cut(s)	1516, 1526, 1924, 1938, 1939
AsuHPI	GGTGA	4 cut(s)	24, 218, 1415, 2095
AsuII	TTCGAA	2 cut(s)	1017, 2058
AsuNHI	GCTAGC	1 cut(s)	371
AvaI	CYCGRG	2 cut(s)	234, 1928
AvaII	GGWCC	1 cut(s)	1526
BaeGI	GKGCMC	1 cut(s)	1942
BaeI	ACNNNNGTAYC	2 cut(s)	738, 771
BalI	TGGCCA	1 cut(s)	146
BanII	GRGCYC	2 cut(s)	1441, 1942
BarI	GAAGNNNNNNTAC	2 cut(s)	730, 762
BauI	CACGAG	1 cut(s)	1605
BbsI	GAAGAC	2 cut(s)	735, 1324
Bbv12I	GWGCWC	1 cut(s)	1393
BbvI	GCAGC	8 cut(s)	296, 515, 667, 953, 1033, 1037, 1280, 2110
BccI	CCATC	5 cut(s)	371, 1157, 1184, 1594, 1739
BceAI	ACGGC	4 cut(s)	107, 112, 1274, 1503
BcgI	CGANNNNNNTGC	4 cut(s)	2022, 2048, 2056, 2082
BciT130I	CCWGG	3 cut(s)	989, 1004, 1564
BciVI	GTATCC	3 cut(s)	487, 1571, 1622
BclI	TGATCA	1 cut(s)	61
BcoDI	GTCTC	2 cut(s)	623, 1716
BcuI	ACTAGT	1 cut(s)	749
BfaI	CTAG	3 cut(s)	372, 596, 750
BfmI	CTRYAG	3 cut(s)	939, 1047, 1272
BfoI	RGCGCY	1 cut(s)	978
BfuAI	ACCTGC	3 cut(s)	142, 203, 1040
BfuI	GTATCC	3 cut(s)	487, 1571, 1622
BisI	GCNGC	9 cut(s)	285, 504, 681, 942, 1026, 1047, 1269, 1830, 2124
BlsI	GCNGC	9 cut(s)	286, 505, 682, 943, 1027, 1048, 1270, 1831, 2125
BmcAI	AGTACT	1 cut(s)	314
Bme1390I	CCNGG	3 cut(s)	989, 1004, 1564
Bme18I	GGWCC	1 cut(s)	1526
BmeT110I	CYCGRG	2 cut(s)	234, 1928
BmgT120I	GGNCC	5 cut(s)	1516, 1526, 1924, 1938, 1939
BmiI	GGNNCC	6 cut(s)	271, 960, 1117, 1164, 1440, 1940
BmrFI	CCNGG	3 cut(s)	989, 1004, 1564
BmsI	GCATC	7 cut(s)	470, 595, 794, 1029, 1117, 1739, 1847
BmtI	GCTAGC	1 cut(s)	375
BpiI	GAAGAC	2 cut(s)	735, 1324
BpmI	CTGGAG	3 cut(s)	1500, 1740, 1775
Bpu14I	TTCGAA	2 cut(s)	1017, 2058
BpuEI	CTTGAG	1 cut(s)	676
BsaI	GGTCTC	1 cut(s)	623
BsaJI	CCNNGG	6 cut(s)	279, 1002, 1119, 1813, 2025, 2093
BsaWI	WCCGGW	3 cut(s)	34, 2101, 2108
BsaXI	ACNNNNNCTCC	2 cut(s)	177, 207
Bsc4I	CCNNNNNNNGG	6 cut(s)	266, 968, 1400, 1819, 2026, 2108
Bse118I	RCCGGY	1 cut(s)	34
Bse1I	ACTGG	6 cut(s)	191, 763, 1026, 1269, 1723, 2052
Bse3DI	GCAATG	2 cut(s)	228, 235
BseAI	TCCGGA	1 cut(s)	2108
BseBI	CCWGG	3 cut(s)	989, 1004, 1564
BseDI	CCNNGG	6 cut(s)	279, 1002, 1119, 1813, 2025, 2093
BseGI	GGATG	6 cut(s)	610, 785, 1327, 1507, 1738, 1754
BseLI	CCNNNNNNNGG	6 cut(s)	266, 968, 1400, 1819, 2026, 2108
BseMI	GCAATG	2 cut(s)	228, 235
BseMII	CTCAG	6 cut(s)	234, 275, 528, 1020, 1485, 2079
BseNI	ACTGG	6 cut(s)	191, 763, 1026, 1269, 1723, 2052
BseRI	GAGGAG	1 cut(s)	198
BseSI	GKGCMC	1 cut(s)	1942
BseXI	GCAGC	8 cut(s)	296, 515, 667, 953, 1033, 1037, 1280, 2110
BsgI	GTGCAG	1 cut(s)	522
Bsh1236I	CGCG	1 cut(s)	525
BshFI	GGCC	5 cut(s)	146, 1518, 1925, 1940, 2156
BshTI	ACCGGT	1 cut(s)	34
BsiHKAI	GWGCWC	1 cut(s)	1393
BsiHKCI	CYCGRG	2 cut(s)	234, 1928
BsiSI	CCGG	4 cut(s)	35, 559, 2102, 2109
BslFI	GGGAC	2 cut(s)	1895, 2002
BslI	CCNNNNNNNGG	6 cut(s)	266, 968, 1400, 1819, 2026, 2108
BsmAI	GTCTC	2 cut(s)	623, 1716
BsmFI	GGGAC	2 cut(s)	1895, 2002
BsmI	GAATGC	1 cut(s)	1365
BsnI	GGCC	5 cut(s)	146, 1518, 1925, 1940, 2156
Bso31I	GGTCTC	1 cut(s)	623
BsoBI	CYCGRG	2 cut(s)	234, 1928
Bsp119I	TTCGAA	2 cut(s)	1017, 2058
Bsp120I	GGGCCC	1 cut(s)	1938
Bsp1286I	GDGCHC	3 cut(s)	1393, 1441, 1942
Bsp13I	TCCGGA	1 cut(s)	2108
Bsp143I	GATC	6 cut(s)	61, 442, 648, 1177, 1876, 2148
Bsp19I	CCATGG	1 cut(s)	279
BspACI	CCGC	5 cut(s)	523, 944, 1160, 1829, 2121
BspANI	GGCC	5 cut(s)	146, 1518, 1925, 1940, 2156
BspCNI	CTCAG	7 cut(s)	4, 235, 274, 527, 1021, 1486, 2080
BspEI	TCCGGA	1 cut(s)	2108
BspFNI	CGCG	1 cut(s)	525
BspHI	TCATGA	2 cut(s)	906, 1152
BspLI	GGNNCC	6 cut(s)	271, 960, 1117, 1164, 1440, 1940
BspMAI	CTGCAG	2 cut(s)	943, 1051
BspMI	ACCTGC	3 cut(s)	142, 203, 1040
BspOI	GCTAGC	1 cut(s)	375
BspPI	GGATC	1 cut(s)	1871
BspT104I	TTCGAA	2 cut(s)	1017, 2058
BspTNI	GGTCTC	1 cut(s)	623
BsrDI	GCAATG	2 cut(s)	228, 235
BsrFI	RCCGGY	1 cut(s)	34
BsrI	ACTGG	6 cut(s)	191, 763, 1026, 1269, 1723, 2052
BssAI	RCCGGY	1 cut(s)	34
BssECI	CCNNGG	6 cut(s)	279, 1002, 1119, 1813, 2025, 2093
BssMI	GATC	6 cut(s)	61, 442, 648, 1177, 1876, 2148
BssNAI	GTATAC	2 cut(s)	873, 985
BssSI	CACGAG	1 cut(s)	1605
BssT1I	CCWWGG	5 cut(s)	279, 1119, 1813, 2025, 2093
Bst1107I	GTATAC	2 cut(s)	873, 985
Bst2BI	CACGAG	1 cut(s)	1605
Bst2UI	CCWGG	3 cut(s)	989, 1004, 1564
Bst4CI	ACNGT	8 cut(s)	47, 151, 574, 843, 955, 1513, 1634, 1999
BstAPI	GCANNNNNTGC	1 cut(s)	218
BstBI	TTCGAA	2 cut(s)	1017, 2058
BstC8I	GCNNGC	3 cut(s)	373, 451, 1044
BstDEI	CTNAG	8 cut(s)	12, 243, 261, 514, 1029, 1230, 1494, 2088
BstDSI	CCRYGG	1 cut(s)	279
BstEII	GGTNACC	1 cut(s)	2116
BstENI	CCTNNNNNAGG	3 cut(s)	264, 1817, 2024
BstF5I	GGATG	6 cut(s)	610, 785, 1327, 1507, 1738, 1754
BstFNI	CGCG	1 cut(s)	525
BstH2I	RGCGCY	1 cut(s)	978
BstHHI	GCGC	4 cut(s)	228, 527, 977, 1707
BstKTI	GATC	6 cut(s)	64, 445, 651, 1180, 1879, 2151
BstMAI	GTCTC	2 cut(s)	623, 1716
BstMBI	GATC	6 cut(s)	61, 442, 648, 1177, 1876, 2148
BstMWI	GCNNNNNNNGC	7 cut(s)	143, 218, 938, 1166, 1835, 1922, 1946
BstNI	CCWGG	3 cut(s)	989, 1004, 1564
BstPI	GGTNACC	1 cut(s)	2116
BstSCI	CCNGG	3 cut(s)	987, 1002, 1562
BstSFI	CTRYAG	3 cut(s)	939, 1047, 1272
BstSLI	GKGCMC	1 cut(s)	1942
BstUI	CGCG	1 cut(s)	525
BstV1I	GCAGC	8 cut(s)	296, 515, 667, 953, 1033, 1037, 1280, 2110
BstV2I	GAAGAC	2 cut(s)	735, 1324
BstXI	CCANNNNNNTGG	1 cut(s)	280
BstZ17I	GTATAC	2 cut(s)	873, 985
BsuI	GTATCC	3 cut(s)	487, 1571, 1622
BsuRI	GGCC	5 cut(s)	146, 1518, 1925, 1940, 2156
BtgI	CCRYGG	1 cut(s)	279
BtgZI	GCGATG	1 cut(s)	390
BtsCI	GGATG	6 cut(s)	610, 785, 1327, 1507, 1738, 1754
BtsIMutI	CAGTG	3 cut(s)	147, 1639, 2008
BveI	ACCTGC	3 cut(s)	142, 203, 1040
Cac8I	GCNNGC	3 cut(s)	373, 451, 1044
CciI	TCATGA	2 cut(s)	906, 1152
CfoI	GCGC	4 cut(s)	228, 527, 977, 1707
Cfr10I	RCCGGY	1 cut(s)	34
Cfr13I	GGNCC	5 cut(s)	1516, 1526, 1924, 1938, 1939
CseI	GACGC	2 cut(s)	1371, 2028
Csp6I	GTAC	5 cut(s)	125, 313, 885, 1546, 1804
CspAI	ACCGGT	1 cut(s)	34
CspCI	CAANNNNNGTGG	2 cut(s)	136, 171
CviQI	GTAC	5 cut(s)	125, 313, 885, 1546, 1804
DdeI	CTNAG	8 cut(s)	12, 243, 261, 514, 1029, 1230, 1494, 2088
DpnI	GATC	6 cut(s)	63, 444, 650, 1179, 1878, 2150
DpnII	GATC	6 cut(s)	61, 442, 648, 1177, 1876, 2148
DrdI	GACNNNNNNGTC	1 cut(s)	2104
DseDI	GACNNNNNNGTC	1 cut(s)	2104
EaeI	YGGCCR	1 cut(s)	144
Eco130I	CCWWGG	5 cut(s)	279, 1119, 1813, 2025, 2093
Eco24I	GRGCYC	2 cut(s)	1441, 1942
Eco31I	GGTCTC	1 cut(s)	623
Eco32I	GATATC	1 cut(s)	1780
Eco47I	GGWCC	1 cut(s)	1526
Eco47III	AGCGCT	1 cut(s)	976
Eco57I	CTGAAG	1 cut(s)	816
Eco88I	CYCGRG	2 cut(s)	234, 1928
Eco91I	GGTNACC	1 cut(s)	2116
EcoNI	CCTNNNNNAGG	3 cut(s)	264, 1817, 2024
EcoO109I	RGGNCCY	1 cut(s)	1924
EcoO65I	GGTNACC	1 cut(s)	2116
EcoRII	CCWGG	3 cut(s)	987, 1002, 1562
EcoRV	GATATC	1 cut(s)	1780
EcoT14I	CCWWGG	5 cut(s)	279, 1119, 1813, 2025, 2093
EcoT38I	GRGCYC	2 cut(s)	1441, 1942
ErhI	CCWWGG	5 cut(s)	279, 1119, 1813, 2025, 2093
FaqI	GGGAC	2 cut(s)	1895, 2002
FauI	CCCGC	1 cut(s)	530
FbaI	TGATCA	1 cut(s)	61
FblI	GTMKAC	3 cut(s)	872, 892, 984
Fnu4HI	GCNGC	9 cut(s)	285, 504, 681, 942, 1026, 1047, 1269, 1830, 2124
FokI	GGATG	6 cut(s)	617, 772, 1334, 1514, 1725, 1761
FriOI	GRGCYC	2 cut(s)	1441, 1942
Fsp4HI	GCNGC	9 cut(s)	285, 504, 681, 942, 1026, 1047, 1269, 1830, 2124
FspBI	CTAG	3 cut(s)	372, 596, 750
FspI	TGCGCA	1 cut(s)	227
GlaI	GCGC	4 cut(s)	227, 526, 976, 1706
GluI	GCNGC	9 cut(s)	285, 504, 681, 942, 1026, 1047, 1269, 1830, 2124
GsuI	CTGGAG	3 cut(s)	1500, 1740, 1775
HaeII	RGCGCY	1 cut(s)	978
HaeIII	GGCC	5 cut(s)	146, 1518, 1925, 1940, 2156
HapII	CCGG	4 cut(s)	35, 559, 2102, 2109
HgaI	GACGC	2 cut(s)	1371, 2028
HhaI	GCGC	4 cut(s)	228, 527, 977, 1707
Hin6I	GCGC	4 cut(s)	226, 525, 975, 1705
HinP1I	GCGC	4 cut(s)	226, 525, 975, 1705
HindIII	AAGCTT	1 cut(s)	1553
HpaII	CCGG	4 cut(s)	35, 559, 2102, 2109
HphI	GGTGA	4 cut(s)	24, 218, 1415, 2095
Hpy166II	GTNNAC	6 cut(s)	400, 839, 873, 893, 985, 1855
Hpy188III	TCNNGA	8 cut(s)	236, 907, 1153, 1187, 1336, 1742, 1930, 2109
Hpy8I	GTNNAC	6 cut(s)	400, 839, 873, 893, 985, 1855
Hpy99I	CGWCG	1 cut(s)	2044
HpyAV	CCTTC	9 cut(s)	348, 375, 388, 892, 1271, 1544, 1565, 1690, 1768
HpyCH4III	ACNGT	8 cut(s)	47, 151, 574, 843, 955, 1513, 1634, 1999
HpyCH4IV	ACGT	1 cut(s)	1860
HpyF10VI	GCNNNNNNNGC	7 cut(s)	143, 218, 938, 1166, 1835, 1922, 1946
HpyF3I	CTNAG	8 cut(s)	12, 243, 261, 514, 1029, 1230, 1494, 2088
HpySE526I	ACGT	1 cut(s)	1860
HspAI	GCGC	4 cut(s)	226, 525, 975, 1705
Kpn2I	TCCGGA	1 cut(s)	2108
Ksp22I	TGATCA	1 cut(s)	61
Kzo9I	GATC	6 cut(s)	61, 442, 648, 1177, 1876, 2148
LmnI	GCTCC	4 cut(s)	269, 1396, 1444, 1481
Lsp1109I	GCAGC	8 cut(s)	296, 515, 667, 953, 1033, 1037, 1280, 2110
LweI	GCATC	7 cut(s)	470, 595, 794, 1029, 1117, 1739, 1847
MaeI	CTAG	3 cut(s)	372, 596, 750
MaeII	ACGT	1 cut(s)	1860
MaeIII	GTNAC	8 cut(s)	364, 577, 754, 1137, 1636, 1646, 2083, 2116
MalI	GATC	6 cut(s)	63, 444, 650, 1179, 1878, 2150
MboI	GATC	6 cut(s)	61, 442, 648, 1177, 1876, 2148
MboII	GAAGA	6 cut(s)	175, 547, 740, 1324, 1783, 2143
MfeI	CAATTG	1 cut(s)	1059
MhlI	GDGCHC	3 cut(s)	1393, 1441, 1942
MlsI	TGGCCA	1 cut(s)	146
MluCI	AATT	8 cut(s)	18, 359, 507, 612, 1059, 1758, 1846, 1905
MluNI	TGGCCA	1 cut(s)	146
MlyI	GAGTC	5 cut(s)	829, 912, 973, 1040, 2114
Mox20I	TGGCCA	1 cut(s)	146
MroI	TCCGGA	1 cut(s)	2108
MscI	TGGCCA	1 cut(s)	146
MslI	CAYNNNNRTG	4 cut(s)	278, 1511, 1730, 1743
Msp20I	TGGCCA	1 cut(s)	146
MspA1I	CMGCKG	5 cut(s)	944, 1028, 1160, 1271, 2123
MspI	CCGG	4 cut(s)	35, 559, 2102, 2109
MspR9I	CCNGG	3 cut(s)	989, 1004, 1564
MunI	CAATTG	1 cut(s)	1059
Mva1269I	GAATGC	1 cut(s)	1365
MvaI	CCWGG	3 cut(s)	989, 1004, 1564
MvnI	CGCG	1 cut(s)	525
MwoI	GCNNNNNNNGC	7 cut(s)	143, 218, 938, 1166, 1835, 1922, 1946
NcoI	CCATGG	1 cut(s)	279
NdeII	GATC	6 cut(s)	61, 442, 648, 1177, 1876, 2148
NheI	GCTAGC	1 cut(s)	371
NlaIV	GGNNCC	6 cut(s)	271, 960, 1117, 1164, 1440, 1940
NmeAIII	GCCGAG	1 cut(s)	1999
NmuCI	GTSAC	2 cut(s)	577, 2083
NsbI	TGCGCA	1 cut(s)	227
NspV	TTCGAA	2 cut(s)	1017, 2058
OliI	CACNNNNGTG	1 cut(s)	1511
PaeR7I	CTCGAG	1 cut(s)	1928
PagI	TCATGA	2 cut(s)	906, 1152
PcsI	WCGNNNNNNNCGW	1 cut(s)	959
PctI	GAATGC	1 cut(s)	1365
PfeI	GAWTC	6 cut(s)	8, 552, 656, 1234, 1406, 1416
PflFI	GACNNNGTC	1 cut(s)	1859
PfoI	TCCNGGA	1 cut(s)	1562
PinAI	ACCGGT	1 cut(s)	34
PkrI	GCNGC	9 cut(s)	286, 505, 682, 943, 1027, 1048, 1270, 1831, 2125
PleI	GAGTC	5 cut(s)	828, 911, 972, 1039, 2113
PpsI	GAGTC	5 cut(s)	828, 911, 972, 1039, 2113
Psp6I	CCWGG	3 cut(s)	987, 1002, 1562
PspEI	GGTNACC	1 cut(s)	2116
PspGI	CCWGG	3 cut(s)	987, 1002, 1562
PspN4I	GGNNCC	6 cut(s)	271, 960, 1117, 1164, 1440, 1940
PspOMI	GGGCCC	1 cut(s)	1938
PspPI	GGNCC	5 cut(s)	1516, 1526, 1924, 1938, 1939
PstI	CTGCAG	2 cut(s)	943, 1051
PsyI	GACNNNGTC	1 cut(s)	1859
PvuII	CAGCTG	2 cut(s)	1028, 1271
RsaI	GTAC	5 cut(s)	126, 314, 886, 1547, 1805
RsaNI	GTAC	5 cut(s)	125, 313, 885, 1546, 1804
RseI	CAYNNNNRTG	4 cut(s)	278, 1511, 1730, 1743
SatI	GCNGC	9 cut(s)	285, 504, 681, 942, 1026, 1047, 1269, 1830, 2124
Sau3AI	GATC	6 cut(s)	61, 442, 648, 1177, 1876, 2148
Sau96I	GGNCC	5 cut(s)	1516, 1526, 1924, 1938, 1939
ScaI	AGTACT	1 cut(s)	314
SchI	GAGTC	5 cut(s)	829, 912, 973, 1040, 2114
ScrFI	CCNGG	3 cut(s)	989, 1004, 1564
SduI	GDGCHC	3 cut(s)	1393, 1441, 1942
SfaNI	GCATC	7 cut(s)	470, 595, 794, 1029, 1117, 1739, 1847
SfcI	CTRYAG	3 cut(s)	939, 1047, 1272
Sfr274I	CTCGAG	1 cut(s)	1928
SfuI	TTCGAA	2 cut(s)	1017, 2058
SgrAI	CRCCGGYG	1 cut(s)	34
SinI	GGWCC	1 cut(s)	1526
SlaI	CTCGAG	1 cut(s)	1928
SmiMI	CAYNNNNRTG	4 cut(s)	278, 1511, 1730, 1743
SmlI	CTYRAG	2 cut(s)	691, 1928
SmoI	CTYRAG	2 cut(s)	691, 1928
SpeI	ACTAGT	1 cut(s)	749
Sse9I	AATT	8 cut(s)	18, 359, 507, 612, 1059, 1758, 1846, 1905
SsiI	CCGC	5 cut(s)	523, 944, 1160, 1829, 2121
SspI	AATATT	2 cut(s)	568, 1426
SspMI	CTAG	3 cut(s)	372, 596, 750
StyD4I	CCNGG	3 cut(s)	987, 1002, 1562
StyI	CCWWGG	5 cut(s)	279, 1119, 1813, 2025, 2093
TaaI	ACNGT	8 cut(s)	47, 151, 574, 843, 955, 1513, 1634, 1999
TaiI	ACGT	1 cut(s)	1863
TaqI	TCGA	6 cut(s)	641, 1017, 1186, 1929, 2042, 2058
TasI	AATT	8 cut(s)	18, 359, 507, 612, 1059, 1758, 1846, 1905
TatI	WGTACW	3 cut(s)	312, 884, 1803
TauI	GCSGC	1 cut(s)	1832
TfiI	GAWTC	6 cut(s)	8, 552, 656, 1234, 1406, 1416
TscAI	CASTG	3 cut(s)	154, 1639, 2008
TseFI	GTSAC	2 cut(s)	577, 2083
TseI	GCWGC	8 cut(s)	284, 503, 680, 941, 1025, 1046, 1268, 2123
Tsp45I	GTSAC	2 cut(s)	577, 2083
TspDTI	ATGAA	5 cut(s)	429, 1408, 1727, 1959, 2144
TspRI	CASTG	3 cut(s)	154, 1639, 2008
Tth111I	GACNNNGTC	1 cut(s)	1859
VpaK11BI	GGWCC	1 cut(s)	1526
XagI	CCTNNNNNAGG	3 cut(s)	264, 1817, 2024
XapI	RAATTY	1 cut(s)	359
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2077
XhoI	CTCGAG	1 cut(s)	1928
XmiI	GTMKAC	3 cut(s)	872, 892, 984
XspI	CTAG	3 cut(s)	372, 596, 750
ZrmI	AGTACT	1 cut(s)	314

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.