Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0002434

Physical Location & Seq

Genomic Coordinates Forward (+)

76537536 .. 76542322

Gene Structure 4787 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG535300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2361 bp

ATGAGTTTGAGTGATTTCAAAGAAGAAGCGAAAATTTCGTTTTCCTTTTGCGCGCGTTTTGTGTTAGTTGCTGTTGAATTGAAAAGCATACGTGGTGCCGCATTGCGGTGGAACTTGATTTTTTTGTTCCATCATCTGTGCACTGCATACTGGATTTTGTTTAGTTCTGTGATGGAGGATTCTGAAGGGAGCGTGTTGAACTCACCGGTTGAAGAAAACTTGGGTGCTAGTGATCTCTTGTCAATTCTGAAAGGCTATGGAGCATTTGGGATATTGGGTGCTGTTATTATTGCCCTACTTGTACCTTTGTTGCTATCAACTGTGTTCCTGGGAAAGAAAAGGAAGCAACGAGGAGTTCCCGTGCAAGTTGGTGGTGAGGAAGGTTATGCAATGCGCAACATTCGTGTTACTGACTTGATTGAAACTCCTCATAAAGAGGCGACAACTGTGCCAGCCTTGTTTGAGCAATCTTGTGAAAAGTACGCACAGAATCAGTTTCTTGGAACCAGGAAGTTGATTGGAAGGGAATTTGTCACAGCTAGCAATGGCAAGAAGTTTGAGAAACTTCACTTAGGAGATTATGAATGGCAAACTTACAGACAAGTCTTTGATCGTGCTTGCAACTTTGCATCTGGCCTTATTAAGTTAGGTCATAATGTGGATACCCGTGCTGCAATTTTTGCTGACACCTGCGCAGAGTGGTTTATAACCTTTCAGGGGTGCTTCCGGCAGAATATTACTGTTGTTACGATCTATTCTTCCCTAGGTGAGGATGCACTAATCCACTCGCTTAATGAGACTCAAGTTTCAACATTGATCTGTGATTCAAAGCAGTTGAAAAAGCTGGCTTCCATAAGCTCAAGGCTAAATAGCATAAAAAATGTAATTTATTTTGAAGACGAGGGAAGTTCAGATGCTAAAGAATTTGAAAGTACTACCAATTGGACGGTCAGATCCATTTCTGAAGTTGAGAAACTTGGACAAGAAAATTCTGTTCATCCACGTTTACCTTCTAAGAATGACATCGCTGTTATCATGTATACGAGCGGCAGTACAGGTCTACCAAAGGGGGTCATGATAACTCATGGCAATATTGTAGCCACTACGGCAGCTGTTACGACACTGATTCCAAGTCTGGGTGGCACTGATGCATACTTAGCATACTTGCCCTTAGCTCATGTTTTTGAATTGGCCGCGGAGTCTGTGATGATGGCTTCAGGTGTTAAAGTTGGTTATGGTTCAGCATTGACTTTAACAGACACTTCAAATAAAATTAAGAAAGGAACCAAGGGAGATGTTTCTATGTTAAAGCCGACTCTCATGACTACAGTTCCTGCTATTATAGATCGTATTCGTGATGCAGTTTTTAAAAAGGTTGAGGAGAAAGGGGGGATAGCGAAGAACCTTTTTGACATTGGATTTAAACGGCGACTGGCTGCTGTTGAAGGAAGCTGGTTTGGTGCTTGGGGTCTGGAAAAATTGTTTTGGGATAATTTTGTCTTTAAAGTTATACGTCTAGGGCTGGGAGGGCGCATTCGCTTTATGATCTGTGGTGGAGCCCCTTTATCTGCTGATTCACAACGATTCATCAATATCTGCATGGGTGCTCCAATCGGGCAAGCATATGGCTTGACAGAAACATTTGCTGGAGCTACGTTTTCTGAGTGGGATGACACAGCAGTGGGCCGTGTTGGCCCACCATTGCCTAGTTGCTACATTAAGCTTGTTTCTTGGAAAGAAGGTGGATACTTGGCATCTGACAAGCCAATGCCTCGTGGGGAGATTGTAATTGGGGGACCCTGTGTAACTGCTGGGTACTTTAAGAATCAAGAAAAGACTGACGAGGTCTACAAGGTTGATGAGAATGGCATGCGCTGGTTTTATACTGGTGACATTGGAATGTTTCATCCTGATGGATGCCTTGAAATTATAGATAGGAAGAAGGATATCGTTAAACTCCAGCATGGAGAATACATCTCCCTCGGAAAGGTTGAGGCAGCATTGATAAACAGTAATTATGTGGACAATATCATGGTATTTGCAGACCCCTTCCACAACTACTGCGTAGCTGTAGTTGTCCCTGTGCGTCAAGCCCTGGAGCAGTGGGCTCAGGGAGCTGGAATCAAGTACAATGATTTTTCTGAGCTGTGTGGAAAAGCTGAAGCAGTTAGTGAGGTCCAGCAATCCCTCTCCAAGGTAGCAAAAGAAGCAAAATTGGACAAGTTTGAACTTCCTGGAAAGATTTATTTGGCGCCTGATCCATGGACACCTGAATCCGGATTGGTTACTGCTGCTCTCAAGATCAAGAGGGAACAGATCAAGTCCAAGTTCAAAAGTGAGATTGAGAAGTTGTATGGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001676 GO:0003674 GO:0003824 GO:0004467 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006082 GO:0006629 GO:0006631 GO:0008150 GO:0008152 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0012505 GO:0015645 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0019752 GO:0031967 GO:0031975 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00061 ko00071 ko01100 ko01212 ko02024 ko03320 ko04146 ko04216 ko04714 ko04920 map00061 map00071 map01100 map01212 map02024 map03320 map04146 map04216 map04714 map04920

Pfam Domains

Protein Families

AMP-binding

Protein Analysis

786

Amino Acids

86.59

Weight (kDa)

7.49

Isoelectric Point (pI)

27.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	187 - 607	6e-86	AMP-binding enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002434)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04350 AT2G04350
fragaria_vesca	FvH4_6g40470 FvH4_6g40470 FvH4_6g40470 FvH4_6g40470 FvH4_6g40470 FvH4_6g40470 FvH4_6g40470 FvH4_6g40470
malus_domestica	MD09G1129700.v1.1 MD17G1118500.v1.1
prunus_persica	Prupe.3G195600_v2.0.a1 Prupe.3G195600_v2.0.a1 Prupe.3G195600_v2.0.a1 Prupe.3G195600_v2.0.a1
pyrus_communis	pycom09g05270 pycom17g10890
rosa_chinensis	RchiOBHm_Chr2g0155701 RchiOBHm_Chr2g0155721
rosa_laevigata	RLG00000020855 RLG00000020857 RLG00000020858
rosa_multiflora	Rmu_co8272995.1_g000001 Rmu_co8427559.1_g000001 Rmu_sc0002712.1_g000001 Rmu_sc0011953.1_g000003 Rmu_sc0011953.1_g000015
rosa_roxburghii	Rroxscaffold_2G00093540
rosa_rugosa	Rorug02G0449500
rosa_samantha	Rh2AG515400 Rh2AG515500 Rh2BG526400 Rh2BG526500 Rh2CG500300 Rh2CG500500 Rh2DG535300 Rh2DG535500
rosa_wichuraiana	Rw2G042360 Rw2G042370 Rw2G042390

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	707
AarI	CACCTGC	1 cut(s)	698
Acc16I	TGCGCA	2 cut(s)	395, 694
Acc36I	ACCTGC	1 cut(s)	698
AccB1I	GGYRCC	2 cut(s)	95, 2251
AccBSI	CCGCTC	1 cut(s)	1047
AccI	GTMKAC	3 cut(s)	1040, 1060, 1848
AccII	CGCG	3 cut(s)	53, 55, 1196
AccIII	TCCGGA	1 cut(s)	2276
AciI	CCGC	5 cut(s)	99, 106, 1047, 1194, 1196
AclWI	GGATC	2 cut(s)	948, 2252
AcoI	YGGCCR	1 cut(s)	1191
AcsI	RAATTY	4 cut(s)	33, 527, 923, 988
AcuI	CTGAAG	4 cut(s)	204, 984, 1200, 2181
AcyI	GRCGYC	1 cut(s)	2252
AfaI	GTAC	6 cut(s)	303, 482, 934, 1054, 1817, 2129
AfiI	CCNNNNNNNGG	7 cut(s)	105, 717, 769, 1136, 1987, 2194, 2276
AgeI	ACCGGT	1 cut(s)	205
AjnI	CCWGG	4 cut(s)	327, 506, 2094, 2233
AleI	CACNNNNGTG	1 cut(s)	1679
Alw21I	GWGCWC	2 cut(s)	143, 1609
Alw26I	GTCTC	1 cut(s)	791
Alw44I	GTGCAC	1 cut(s)	139
AlwI	GGATC	2 cut(s)	948, 2252
AlwNI	CAGNNNCTG	1 cut(s)	1334
Aor13HI	TCCGGA	1 cut(s)	2276
AoxI	GGCC	4 cut(s)	634, 1191, 1684, 1693
ApaLI	GTGCAC	1 cut(s)	139
ApeKI	GCWGC	5 cut(s)	671, 1109, 1436, 1997, 2291
ApoI	RAATTY	4 cut(s)	33, 527, 923, 988
AsiGI	ACCGGT	1 cut(s)	205
AspA2I	CCTAGG	1 cut(s)	763
AspLEI	GCGC	7 cut(s)	53, 55, 396, 695, 1533, 1875, 2254
AspS9I	GGNCC	4 cut(s)	1684, 1694, 1796, 2176
AsuHPI	GGTGA	4 cut(s)	195, 386, 779, 1901
AsuNHI	GCTAGC	1 cut(s)	539
AvaII	GGWCC	2 cut(s)	1796, 2176
AvrII	CCTAGG	1 cut(s)	763
BaeGI	GKGCMC	1 cut(s)	143
BanI	GGYRCC	2 cut(s)	95, 2251
BanII	GRGCYC	2 cut(s)	1561, 2110
BauI	CACGAG	1 cut(s)	1773
BbsI	GAAGAC	1 cut(s)	903
Bbv12I	GWGCWC	2 cut(s)	143, 1609
BbvI	GCAGC	5 cut(s)	658, 1121, 1423, 2009, 2278
BccI	CCATC	4 cut(s)	138, 166, 1204, 1907
BceAI	ACGGC	3 cut(s)	1122, 1442, 1671
BcgI	CGANNNNNNTGC	2 cut(s)	430, 464
BciT130I	CCWGG	4 cut(s)	329, 508, 2096, 2235
BciVI	GTATCC	2 cut(s)	655, 1739
BcoDI	GTCTC	1 cut(s)	791
BfaI	CTAG	5 cut(s)	228, 540, 764, 1517, 1707
BfmI	CTRYAG	2 cut(s)	1326, 2070
BfoI	RGCGCY	1 cut(s)	2255
BfuAI	ACCTGC	1 cut(s)	698
BfuI	GTATCC	2 cut(s)	655, 1739
BglI	GCCNNNNNGGC	2 cut(s)	1106, 1692
BisI	GCNGC	8 cut(s)	99, 672, 1048, 1110, 1194, 1437, 1998, 2292
BlnI	CCTAGG	1 cut(s)	763
BlsI	GCNGC	8 cut(s)	100, 673, 1049, 1111, 1195, 1438, 1999, 2293
BmcAI	AGTACT	1 cut(s)	934
Bme1390I	CCNGG	4 cut(s)	329, 508, 2096, 2235
Bme18I	GGWCC	2 cut(s)	1796, 2176
BmgT120I	GGNCC	4 cut(s)	1684, 1694, 1796, 2176
BmiI	GGNNCC	7 cut(s)	97, 505, 1285, 1558, 1797, 1798, 2253
BmrFI	CCNGG	4 cut(s)	329, 508, 2096, 2235
BmsI	GCATC	7 cut(s)	638, 763, 904, 1138, 1348, 1763, 1907
BmtI	GCTAGC	1 cut(s)	543
BpiI	GAAGAC	1 cut(s)	903
BpmI	CTGGAG	3 cut(s)	1668, 1943, 2117
Bpu10I	CCTNAGC	2 cut(s)	1171, 2109
BpuEI	CTTGAG	3 cut(s)	786, 844, 2282
BsaAI	YACGTR	1 cut(s)	92
BsaHI	GRCGYC	1 cut(s)	2252
BsaJI	CCNNGG	8 cut(s)	328, 763, 1194, 1287, 1981, 2094, 2193, 2261
BsaWI	WCCGGW	2 cut(s)	205, 2276
BsaXI	ACNNNNNCTCC	2 cut(s)	345, 375
Bsc4I	CCNNNNNNNGG	7 cut(s)	105, 717, 769, 1136, 1987, 2194, 2276
Bse118I	RCCGGY	1 cut(s)	205
Bse1I	ACTGG	3 cut(s)	155, 1437, 1891
Bse3DI	GCAATG	4 cut(s)	101, 396, 550, 1700
BseAI	TCCGGA	1 cut(s)	2276
BseBI	CCWGG	4 cut(s)	329, 508, 2096, 2235
BseDI	CCNNGG	8 cut(s)	328, 763, 1194, 1287, 1981, 2094, 2193, 2261
BseGI	GGATG	5 cut(s)	778, 997, 1675, 1906, 1922
BseLI	CCNNNNNNNGG	7 cut(s)	105, 717, 769, 1136, 1987, 2194, 2276
BseMI	GCAATG	4 cut(s)	101, 396, 550, 1700
BseMII	CTCAG	3 cut(s)	1653, 2123, 2133
BseNI	ACTGG	3 cut(s)	155, 1437, 1891
BsePI	GCGCGC	1 cut(s)	51
BseRI	GAGGAG	3 cut(s)	366, 417, 1394
BseSI	GKGCMC	1 cut(s)	143
BseXI	GCAGC	5 cut(s)	658, 1121, 1423, 2009, 2278
BseYI	CCCAGC	2 cut(s)	1522, 1811
Bsh1236I	CGCG	3 cut(s)	53, 55, 1196
BshFI	GGCC	4 cut(s)	636, 1193, 1686, 1695
BshNI	GGYRCC	2 cut(s)	95, 2251
BshTI	ACCGGT	1 cut(s)	205
BsiHKAI	GWGCWC	2 cut(s)	143, 1609
BsiSI	CCGG	3 cut(s)	206, 727, 2277
BslFI	GGGAC	2 cut(s)	1809, 2063
BslI	CCNNNNNNNGG	7 cut(s)	105, 717, 769, 1136, 1987, 2194, 2276
BsmAI	GTCTC	1 cut(s)	791
BsmFI	GGGAC	2 cut(s)	1809, 2063
BsmI	GAATGC	1 cut(s)	1533
BsnI	GGCC	4 cut(s)	636, 1193, 1686, 1695
Bsp1286I	GDGCHC	4 cut(s)	143, 1561, 1609, 2110
Bsp13I	TCCGGA	1 cut(s)	2276
Bsp19I	CCATGG	1 cut(s)	2261
BspACI	CCGC	5 cut(s)	99, 106, 1047, 1194, 1196
BspANI	GGCC	4 cut(s)	636, 1193, 1686, 1695
BspCNI	CTCAG	3 cut(s)	1654, 2122, 2134
BspEI	TCCGGA	1 cut(s)	2276
BspFNI	CGCG	3 cut(s)	53, 55, 1196
BspHI	TCATGA	2 cut(s)	1074, 1320
BspLI	GGNNCC	7 cut(s)	97, 505, 1285, 1558, 1797, 1798, 2253
BspMI	ACCTGC	1 cut(s)	698
BspOI	GCTAGC	1 cut(s)	543
BspPI	GGATC	2 cut(s)	948, 2252
BspT107I	GGYRCC	2 cut(s)	95, 2251
BsrBI	CCGCTC	1 cut(s)	1047
BsrDI	GCAATG	4 cut(s)	101, 396, 550, 1700
BsrFI	RCCGGY	1 cut(s)	205
BsrI	ACTGG	3 cut(s)	155, 1437, 1891
BssAI	RCCGGY	1 cut(s)	205
BssECI	CCNNGG	8 cut(s)	328, 763, 1194, 1287, 1981, 2094, 2193, 2261
BssHII	GCGCGC	1 cut(s)	51
BssNAI	GTATAC	1 cut(s)	1041
BssNI	GRCGYC	1 cut(s)	2252
BssSI	CACGAG	1 cut(s)	1773
BssT1I	CCWWGG	4 cut(s)	763, 1287, 2193, 2261
Bst1107I	GTATAC	1 cut(s)	1041
Bst2BI	CACGAG	1 cut(s)	1773
Bst2UI	CCWGG	4 cut(s)	329, 508, 2096, 2235
Bst4CI	ACNGT	6 cut(s)	322, 448, 742, 949, 1330, 2012
BstACI	GRCGYC	1 cut(s)	2252
BstAPI	GCANNNNNTGC	1 cut(s)	680
BstBAI	YACGTR	1 cut(s)	92
BstC8I	GCNNGC	7 cut(s)	53, 453, 541, 619, 846, 1620, 1871
BstDEI	CTNAG	7 cut(s)	571, 1014, 1156, 1171, 1662, 2109, 2142
BstDSI	CCRYGG	2 cut(s)	1194, 2261
BstENI	CCTNNNNNAGG	3 cut(s)	767, 1985, 2192
BstF5I	GGATG	5 cut(s)	778, 997, 1675, 1906, 1922
BstFNI	CGCG	3 cut(s)	53, 55, 1196
BstH2I	RGCGCY	1 cut(s)	2255
BstHHI	GCGC	7 cut(s)	53, 55, 396, 695, 1533, 1875, 2254
BstMAI	GTCTC	1 cut(s)	791
BstMWI	GCNNNNNNNGC	7 cut(s)	680, 1106, 1157, 1528, 1692, 2114, 2207
BstNI	CCWGG	4 cut(s)	329, 508, 2096, 2235
BstNSI	RCATGY	1 cut(s)	1873
BstSCI	CCNGG	4 cut(s)	327, 506, 2094, 2233
BstSFI	CTRYAG	2 cut(s)	1326, 2070
BstSLI	GKGCMC	1 cut(s)	143
BstUI	CGCG	3 cut(s)	53, 55, 1196
BstV1I	GCAGC	5 cut(s)	658, 1121, 1423, 2009, 2278
BstV2I	GAAGAC	1 cut(s)	903
BstX2I	RGATCY	1 cut(s)	953
BstYI	RGATCY	1 cut(s)	953
BstZ17I	GTATAC	1 cut(s)	1041
BsuI	GTATCC	2 cut(s)	655, 1739
BsuRI	GGCC	4 cut(s)	636, 1193, 1686, 1695
BtgI	CCRYGG	2 cut(s)	1194, 2261
BtgZI	GCGATG	1 cut(s)	1009
BtsCI	GGATG	5 cut(s)	778, 997, 1675, 1906, 1922
BtsI	GCAGTG	3 cut(s)	141, 1686, 2108
BtsIMutI	CAGTG	5 cut(s)	141, 1121, 1143, 1686, 2108
BveI	ACCTGC	1 cut(s)	698
Cac8I	GCNNGC	7 cut(s)	53, 453, 541, 619, 846, 1620, 1871
CaiI	CAGNNNCTG	1 cut(s)	1334
CciI	TCATGA	2 cut(s)	1074, 1320
CfoI	GCGC	7 cut(s)	53, 55, 396, 695, 1533, 1875, 2254
Cfr10I	RCCGGY	1 cut(s)	205
Cfr13I	GGNCC	4 cut(s)	1684, 1694, 1796, 2176
Cfr42I	CCGCGG	1 cut(s)	1197
CseI	GACGC	1 cut(s)	2075
Csp6I	GTAC	6 cut(s)	302, 481, 933, 1053, 1816, 2128
CspAI	ACCGGT	1 cut(s)	205
CviQI	GTAC	6 cut(s)	302, 481, 933, 1053, 1816, 2128
DdeI	CTNAG	7 cut(s)	571, 1014, 1156, 1171, 1662, 2109, 2142
DinI	GGCGCC	1 cut(s)	2253
DraI	TTTAAA	3 cut(s)	1369, 1423, 1504
EaeI	YGGCCR	1 cut(s)	1191
Eco130I	CCWWGG	4 cut(s)	763, 1287, 2193, 2261
Eco24I	GRGCYC	2 cut(s)	1561, 2110
Eco32I	GATATC	1 cut(s)	1948
Eco47I	GGWCC	2 cut(s)	1796, 2176
Eco57I	CTGAAG	4 cut(s)	204, 984, 1200, 2181
EcoNI	CCTNNNNNAGG	3 cut(s)	767, 1985, 2192
EcoO109I	RGGNCCY	1 cut(s)	1796
EcoRII	CCWGG	4 cut(s)	327, 506, 2094, 2233
EcoRV	GATATC	1 cut(s)	1948
EcoT14I	CCWWGG	4 cut(s)	763, 1287, 2193, 2261
EcoT22I	ATGCAT	1 cut(s)	1153
EcoT38I	GRGCYC	2 cut(s)	1561, 2110
EgeI	GGCGCC	1 cut(s)	2253
EheI	GGCGCC	1 cut(s)	2253
ErhI	CCWWGG	4 cut(s)	763, 1287, 2193, 2261
FaqI	GGGAC	2 cut(s)	1809, 2063
FauNDI	CATATG	1 cut(s)	1624
FblI	GTMKAC	3 cut(s)	1040, 1060, 1848
Fnu4HI	GCNGC	8 cut(s)	99, 672, 1048, 1110, 1194, 1437, 1998, 2292
FokI	GGATG	5 cut(s)	785, 984, 1682, 1893, 1929
FriOI	GRGCYC	2 cut(s)	1561, 2110
Fsp4HI	GCNGC	8 cut(s)	99, 672, 1048, 1110, 1194, 1437, 1998, 2292
FspBI	CTAG	5 cut(s)	228, 540, 764, 1517, 1707
FspI	TGCGCA	2 cut(s)	395, 694
GlaI	GCGC	7 cut(s)	52, 54, 395, 694, 1532, 1874, 2253
GluI	GCNGC	8 cut(s)	99, 672, 1048, 1110, 1194, 1437, 1998, 2292
GsaI	CCCAGC	2 cut(s)	1526, 1815
GsuI	CTGGAG	3 cut(s)	1668, 1943, 2117
HaeII	RGCGCY	1 cut(s)	2255
HaeIII	GGCC	4 cut(s)	636, 1193, 1686, 1695
HapII	CCGG	3 cut(s)	206, 727, 2277
HgaI	GACGC	1 cut(s)	2075
HhaI	GCGC	7 cut(s)	53, 55, 396, 695, 1533, 1875, 2254
Hin1I	GRCGYC	1 cut(s)	2252
Hin6I	GCGC	7 cut(s)	51, 53, 394, 693, 1531, 1873, 2252
HinP1I	GCGC	7 cut(s)	51, 53, 394, 693, 1531, 1873, 2252
HindIII	AAGCTT	1 cut(s)	1721
HpaII	CCGG	3 cut(s)	206, 727, 2277
HphI	GGTGA	4 cut(s)	195, 386, 779, 1901
Hpy166II	GTNNAC	6 cut(s)	141, 1007, 1041, 1061, 1849, 2023
Hpy188I	TCNGA	9 cut(s)	184, 249, 913, 953, 964, 1663, 1759, 1985, 2143
Hpy188III	TCNNGA	9 cut(s)	1075, 1321, 1355, 1472, 1829, 1910, 2277, 2299, 2305
Hpy8I	GTNNAC	6 cut(s)	141, 1007, 1041, 1061, 1849, 2023
HpyAV	CCTTC	8 cut(s)	179, 374, 516, 1020, 1439, 1733, 1936, 2059
HpyCH4III	ACNGT	6 cut(s)	322, 448, 742, 949, 1330, 2012
HpyCH4IV	ACGT	4 cut(s)	91, 1003, 1513, 1655
HpyF10VI	GCNNNNNNNGC	7 cut(s)	680, 1106, 1157, 1528, 1692, 2114, 2207
HpyF3I	CTNAG	7 cut(s)	571, 1014, 1156, 1171, 1662, 2109, 2142
HpySE526I	ACGT	4 cut(s)	91, 1003, 1513, 1655
Hsp92I	GRCGYC	1 cut(s)	2252
HspAI	GCGC	7 cut(s)	51, 53, 394, 693, 1531, 1873, 2252
KasI	GGCGCC	1 cut(s)	2251
KflI	GGGWCCC	1 cut(s)	1796
Kpn2I	TCCGGA	1 cut(s)	2276
KspI	CCGCGG	1 cut(s)	1197
LmnI	GCTCC	7 cut(s)	189, 260, 1556, 1612, 1649, 2098, 2114
Lsp1109I	GCAGC	5 cut(s)	658, 1121, 1423, 2009, 2278
LweI	GCATC	7 cut(s)	638, 763, 904, 1138, 1348, 1763, 1907
MaeI	CTAG	5 cut(s)	228, 540, 764, 1517, 1707
MaeII	ACGT	4 cut(s)	91, 1003, 1513, 1655
MaeIII	GTNAC	7 cut(s)	406, 532, 745, 1114, 1804, 1889, 2284
MbiI	CCGCTC	1 cut(s)	1047
MboII	GAAGA	6 cut(s)	35, 224, 750, 908, 1411, 1951
MfeI	CAATTG	1 cut(s)	940
MflI	RGATCY	1 cut(s)	953
MhlI	GDGCHC	4 cut(s)	143, 1561, 1609, 2110
Mly113I	GGCGCC	1 cut(s)	2252
MlyI	GAGTC	3 cut(s)	793, 1208, 1309
Mph1103I	ATGCAT	1 cut(s)	1153
MroI	TCCGGA	1 cut(s)	2276
MslI	CAYNNNNRTG	4 cut(s)	106, 1679, 1898, 1911
MspA1I	CMGCKG	2 cut(s)	1112, 1196
MspI	CCGG	3 cut(s)	206, 727, 2277
MspR9I	CCNGG	4 cut(s)	329, 508, 2096, 2235
MunI	CAATTG	1 cut(s)	940
Mva1269I	GAATGC	1 cut(s)	1533
MvaI	CCWGG	4 cut(s)	329, 508, 2096, 2235
MvnI	CGCG	3 cut(s)	53, 55, 1196
MwoI	GCNNNNNNNGC	7 cut(s)	680, 1106, 1157, 1528, 1692, 2114, 2207
NarI	GGCGCC	1 cut(s)	2252
NcoI	CCATGG	1 cut(s)	2261
NdeI	CATATG	1 cut(s)	1624
NheI	GCTAGC	1 cut(s)	539
NlaIV	GGNNCC	7 cut(s)	97, 505, 1285, 1558, 1797, 1798, 2253
NmuCI	GTSAC	2 cut(s)	532, 1889
NsbI	TGCGCA	2 cut(s)	395, 694
NsiI	ATGCAT	1 cut(s)	1153
NspI	RCATGY	1 cut(s)	1873
OliI	CACNNNNGTG	1 cut(s)	1679
PaeI	GCATGC	1 cut(s)	1873
PagI	TCATGA	2 cut(s)	1074, 1320
PaqCI	CACCTGC	1 cut(s)	698
PauI	GCGCGC	1 cut(s)	51
PctI	GAATGC	1 cut(s)	1533
PfeI	GAWTC	9 cut(s)	179, 490, 824, 1126, 1574, 1584, 1825, 2121, 2273
PflFI	GACNNNGTC	1 cut(s)	1844
PfoI	TCCNGGA	1 cut(s)	2233
PinAI	ACCGGT	1 cut(s)	205
PkrI	GCNGC	8 cut(s)	100, 673, 1049, 1111, 1195, 1438, 1999, 2293
PleI	GAGTC	3 cut(s)	793, 1207, 1309
PluTI	GGCGCC	1 cut(s)	2255
PpsI	GAGTC	3 cut(s)	793, 1207, 1309
Ppu21I	YACGTR	1 cut(s)	92
PpuMI	RGGWCCY	1 cut(s)	1796
PsiI	TTATAA	1 cut(s)	707
Psp5II	RGGWCCY	1 cut(s)	1796
Psp6I	CCWGG	4 cut(s)	327, 506, 2094, 2233
PspFI	CCCAGC	2 cut(s)	1522, 1811
PspGI	CCWGG	4 cut(s)	327, 506, 2094, 2233
PspN4I	GGNNCC	7 cut(s)	97, 505, 1285, 1558, 1797, 1798, 2253
PspPI	GGNCC	4 cut(s)	1684, 1694, 1796, 2176
PspPPI	RGGWCCY	1 cut(s)	1796
PstNI	CAGNNNCTG	1 cut(s)	1334
PsuI	RGATCY	1 cut(s)	953
PsyI	GACNNNGTC	1 cut(s)	1844
PteI	GCGCGC	1 cut(s)	51
PvuII	CAGCTG	1 cut(s)	1112
RsaI	GTAC	6 cut(s)	303, 482, 934, 1054, 1817, 2129
RsaNI	GTAC	6 cut(s)	302, 481, 933, 1053, 1816, 2128
RseI	CAYNNNNRTG	4 cut(s)	106, 1679, 1898, 1911
SacII	CCGCGG	1 cut(s)	1197
SatI	GCNGC	8 cut(s)	99, 672, 1048, 1110, 1194, 1437, 1998, 2292
Sau96I	GGNCC	4 cut(s)	1684, 1694, 1796, 2176
ScaI	AGTACT	1 cut(s)	934
SchI	GAGTC	3 cut(s)	793, 1208, 1309
ScrFI	CCNGG	4 cut(s)	329, 508, 2096, 2235
SduI	GDGCHC	4 cut(s)	143, 1561, 1609, 2110
SfaNI	GCATC	7 cut(s)	638, 763, 904, 1138, 1348, 1763, 1907
SfcI	CTRYAG	2 cut(s)	1326, 2070
SfiI	GGCCNNNNNGGCC	1 cut(s)	1692
SfoI	GGCGCC	1 cut(s)	2253
Sfr303I	CCGCGG	1 cut(s)	1197
SgrBI	CCGCGG	1 cut(s)	1197
SinI	GGWCC	2 cut(s)	1796, 2176
SmiMI	CAYNNNNRTG	4 cut(s)	106, 1679, 1898, 1911
SmlI	CTYRAG	3 cut(s)	801, 859, 2297
SmoI	CTYRAG	3 cut(s)	801, 859, 2297
SphI	GCATGC	1 cut(s)	1873
SsiI	CCGC	5 cut(s)	99, 106, 1047, 1194, 1196
SspDI	GGCGCC	1 cut(s)	2251
SspI	AATATT	2 cut(s)	736, 1093
SspMI	CTAG	5 cut(s)	228, 540, 764, 1517, 1707
StyD4I	CCNGG	4 cut(s)	327, 506, 2094, 2233
StyI	CCWWGG	4 cut(s)	763, 1287, 2193, 2261
TaaI	ACNGT	6 cut(s)	322, 448, 742, 949, 1330, 2012
TaiI	ACGT	4 cut(s)	94, 1006, 1516, 1658
TatI	WGTACW	3 cut(s)	932, 1052, 2127
TauI	GCSGC	3 cut(s)	101, 1050, 1196
TfiI	GAWTC	9 cut(s)	179, 490, 824, 1126, 1574, 1584, 1825, 2121, 2273
TscAI	CASTG	5 cut(s)	148, 1128, 1150, 1686, 2108
TseFI	GTSAC	2 cut(s)	532, 1889
TseI	GCWGC	5 cut(s)	671, 1109, 1436, 1997, 2291
Tsp45I	GTSAC	2 cut(s)	532, 1889
TspDTI	ATGAA	4 cut(s)	597, 986, 1576, 1895
TspRI	CASTG	5 cut(s)	148, 1128, 1150, 1686, 2108
Tth111I	GACNNNGTC	1 cut(s)	1844
VneI	GTGCAC	1 cut(s)	139
VpaK11BI	GGWCC	2 cut(s)	1796, 2176
XagI	CCTNNNNNAGG	3 cut(s)	767, 1985, 2192
XapI	RAATTY	4 cut(s)	33, 527, 923, 988
XceI	RCATGY	1 cut(s)	1873
XmaJI	CCTAGG	1 cut(s)	763
XmiI	GTMKAC	3 cut(s)	1040, 1060, 1848
XspI	CTAG	5 cut(s)	228, 540, 764, 1517, 1707
ZrmI	AGTACT	1 cut(s)	934
Zsp2I	ATGCAT	1 cut(s)	1153

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.