MD09G1149000.v1.1

Persulfide dioxygenase ETHE1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012170
Physical Location & Seq
Forward (+)
11714888 .. 11718825
3938 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1149000.v1.1.491

Sequence Viewer

Length: 930 bp
ATGCTTCGAATCGGCGTCCTCAGATTCGTCGCTCTCCCTCGCTCCTCCTCCTCCTCCTCGTCTTCTCTTTTGTTCAATCCCTTTTCTCCCAAATCCCTCCCTCCTCCTCCTGTAATCCGCACCAAGCTCGTTTCCCGTTACCGAACCATGGCTTATACGGCGTCGTCTGGTTCTTCTTCCAAGCTCCTCTTCCGCCAGCTCTTCGAGAAGGAGTCCTCCACCTACACTTACTTGCTCGCCGATGTCTCTCACCCCGACAAACCCGCTCTGCTGATTGACCCGGTGGACAAGACGGTGGATAGGGATATCTCCCTTGTCAAAGAACTGGGGTTGAAGTTGATCTACGCCATGAACACTCATGTTCATGCTGATCACGTCACTGGCACTGGCCTCATCAAGACTAAGCTTCCTGGTGTGAAATCTGTCATTTCCAAGGCGAGCAAATCAAAAGCGGACCTTTTGATTGAAGCCGGCGATAAAATATATTTTGGAGATCTGTTTTTGGAGGTTCGAGCTACTCCTGGGCATACATTAGGTTGTCTTACCTATGTTACAGGAGATGGGCCTGATCAACCCCAACCGAGGATGGCTTTCACTGGGGATGCACTACTCATACGTGGATGTGGGAGAACCGATTTTCAGGGTGGCAGTTCACATCAACTCTACAACTCAGTTCATTCACAGATTTTCACATTGCCAAAGGATACATTTATCTATCCTGCTCATGATTACAAGGGATTCACTGTTAGTACTGTGGGGGAGGAGATGCTTTATAATCCTCGGCTCACAAAAGATGAGGAGGGATTTAAAAGTATCATGGAAAATCTGAAACTCTCATACCCAAAAATGATTGACATAGCAGTGCCTGCAAATATGGTTTGTGGATTGCAAGATTTGTCTGAAAGGCCTGCCGAGTCTGTGGCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00920 map00920
Pfam Domains
Protein Families
Lactamase_B

Protein Analysis

310

Amino Acids

33.94

Weight (kDa)

8.93

Isoelectric Point (pI)

33.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lactamase_B PF00753 74 - 242 1.6e-11 Metallo-beta-lactamase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53580 AT1G53580
fragaria_vesca FvH4_6g38610
malus_domestica MD09G1149000.v1.1 MD17G1135300.v1.1
prunus_persica Prupe.3G177200_v2.0.a1
pyrus_communis pycom09g06850 pycom17g12930
rosa_chinensis RchiOBHm_Chr2g0152061
rosa_laevigata RLG00000020603
rosa_multiflora Rmu_sc0000760.1_g000022
rosa_roxburghii Rroxscaffold_2G00096020
rosa_rugosa Rorug02G0430500 Rorug02G0430500
rosa_samantha Rh2AG491300 Rh2BG503700 Rh2CG477700 Rh2DG514900
rosa_wichuraiana Rw2G040390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 774
AccBSI CCGCTC 1 cut(s) 266
AciI CCGC 4 cut(s) 118, 193, 264, 452
AcyI GRCGYC 2 cut(s) 15, 161
AfaI GTAC 1 cut(s) 751
AfiI CCNNNNNNNGG 2 cut(s) 148, 582
AgsI TTSAA 3 cut(s) 76, 334, 467
AjiI CACGTC 1 cut(s) 376
AjnI CCWGG 2 cut(s) 409, 520
AjuI GAANNNNNNNTTGG 2 cut(s) 173, 205
AluBI AGCT 5 cut(s) 127, 184, 199, 406, 515
AluI AGCT 5 cut(s) 127, 184, 199, 406, 515
Alw26I GTCTC 1 cut(s) 250
AlwNI CAGNNNCTG 1 cut(s) 866
AoxI GGCC 3 cut(s) 388, 563, 905
AspS9I GGNCC 2 cut(s) 454, 563
AsuC2I CCSGG 1 cut(s) 281
AsuHPI GGTGA 1 cut(s) 242
AsuII TTCGAA 1 cut(s) 7
AvaII GGWCC 1 cut(s) 454
BarI GAAGNNNNNNTAC 2 cut(s) 326, 358
BbsI GAAGAC 1 cut(s) 54
BccI CCATC 2 cut(s) 554, 580
BceAI ACGGC 1 cut(s) 174
BcgI CGANNNNNNTGC 2 cut(s) 109, 143
BciT130I CCWGG 2 cut(s) 411, 522
BciVI GTATCC 1 cut(s) 697
BclI TGATCA 2 cut(s) 370, 568
BcnI CCSGG 1 cut(s) 281
BcoDI GTCTC 1 cut(s) 250
BfuI GTATCC 1 cut(s) 697
BglII AGATCT 1 cut(s) 493
BmcAI AGTACT 1 cut(s) 751
Bme1390I CCNGG 3 cut(s) 281, 411, 522
Bme18I GGWCC 1 cut(s) 454
BmgBI CACGTC 1 cut(s) 376
BmgT120I GGNCC 2 cut(s) 454, 563
BmrFI CCNGG 3 cut(s) 281, 411, 522
BmrI ACTGGG 2 cut(s) 335, 606
BmsI GCATC 2 cut(s) 592, 756
BmuI ACTGGG 2 cut(s) 335, 606
BpiI GAAGAC 1 cut(s) 54
Bpu14I TTCGAA 1 cut(s) 7
BpuMI CCSGG 1 cut(s) 281
BsaAI YACGTR 1 cut(s) 617
BsaHI GRCGYC 2 cut(s) 15, 161
BsaJI CCNNGG 5 cut(s) 147, 432, 521, 581, 779
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 582
Bse118I RCCGGY 1 cut(s) 470
Bse1I ACTGG 4 cut(s) 330, 385, 391, 601
Bse3DI GCAATG 1 cut(s) 692
BseBI CCWGG 2 cut(s) 411, 522
BseDI CCNNGG 5 cut(s) 147, 432, 521, 581, 779
BseGI GGATG 3 cut(s) 591, 607, 626
BseLI CCNNNNNNNGG 2 cut(s) 148, 582
BseMI GCAATG 1 cut(s) 692
BseMII CTCAG 2 cut(s) 34, 684
BseNI ACTGG 4 cut(s) 330, 385, 391, 601
BshFI GGCC 3 cut(s) 390, 565, 907
BsiSI CCGG 2 cut(s) 281, 471
BslI CCNNNNNNNGG 2 cut(s) 148, 582
BsmAI GTCTC 1 cut(s) 250
BsnI GGCC 3 cut(s) 390, 565, 907
Bsp119I TTCGAA 1 cut(s) 7
Bsp143I GATC 4 cut(s) 339, 370, 493, 568
Bsp19I CCATGG 1 cut(s) 147
BspACI CCGC 4 cut(s) 118, 193, 264, 452
BspANI GGCC 3 cut(s) 390, 565, 907
BspCNI CTCAG 2 cut(s) 33, 683
BspHI TCATGA 1 cut(s) 724
BspQI GCTCTTC 1 cut(s) 206
BspT104I TTCGAA 1 cut(s) 7
BsrBI CCGCTC 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 692
BsrFI RCCGGY 1 cut(s) 470
BsrI ACTGG 4 cut(s) 330, 385, 391, 601
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 5 cut(s) 147, 432, 521, 581, 779
BssMI GATC 4 cut(s) 339, 370, 493, 568
BssNI GRCGYC 2 cut(s) 15, 161
BssT1I CCWWGG 2 cut(s) 147, 432
Bst2UI CCWGG 2 cut(s) 411, 522
Bst4CI ACNGT 3 cut(s) 295, 745, 754
Bst6I CTCTTC 2 cut(s) 194, 206
BstACI GRCGYC 2 cut(s) 15, 161
BstAPI GCANNNNNTGC 1 cut(s) 866
BstBAI YACGTR 1 cut(s) 617
BstBI TTCGAA 1 cut(s) 7
BstC8I GCNNGC 6 cut(s) 197, 237, 439, 472, 867, 909
BstDEI CTNAG 3 cut(s) 20, 402, 670
BstDSI CCRYGG 1 cut(s) 147
BstF5I GGATG 3 cut(s) 591, 607, 626
BstKTI GATC 4 cut(s) 342, 373, 496, 571
BstMAI GTCTC 1 cut(s) 250
BstMBI GATC 4 cut(s) 339, 370, 493, 568
BstMWI GCNNNNNNNGC 2 cut(s) 158, 866
BstNI CCWGG 2 cut(s) 411, 522
BstSCI CCNGG 3 cut(s) 279, 409, 520
BstV2I GAAGAC 1 cut(s) 54
BstX2I RGATCY 1 cut(s) 493
BstYI RGATCY 1 cut(s) 493
BsuI GTATCC 1 cut(s) 697
BsuRI GGCC 3 cut(s) 390, 565, 907
BtgI CCRYGG 1 cut(s) 147
BtrI CACGTC 1 cut(s) 376
BtsCI GGATG 3 cut(s) 591, 607, 626
BtsI GCAGTG 1 cut(s) 867
BtsIMutI CAGTG 5 cut(s) 378, 384, 594, 741, 867
Cac8I GCNNGC 6 cut(s) 197, 237, 439, 472, 867, 909
CaiI CAGNNNCTG 1 cut(s) 866
CciI TCATGA 1 cut(s) 724
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 2 cut(s) 454, 563
CseI GACGC 2 cut(s) 4, 150
Csp6I GTAC 1 cut(s) 750
CviAII CATG 6 cut(s) 148, 349, 359, 365, 725, 817
CviQI GTAC 1 cut(s) 750
DdeI CTNAG 3 cut(s) 20, 402, 670
DpnI GATC 4 cut(s) 341, 372, 495, 570
DpnII GATC 4 cut(s) 339, 370, 493, 568
DraI TTTAAA 1 cut(s) 808
Eam1104I CTCTTC 2 cut(s) 194, 206
EarI CTCTTC 2 cut(s) 194, 206
EciI GGCGGA 1 cut(s) 182
Eco130I CCWWGG 2 cut(s) 147, 432
Eco147I AGGCCT 1 cut(s) 907
Eco32I GATATC 1 cut(s) 307
Eco47I GGWCC 1 cut(s) 454
EcoRII CCWGG 2 cut(s) 409, 520
EcoRV GATATC 1 cut(s) 307
EcoT14I CCWWGG 2 cut(s) 147, 432
ErhI CCWWGG 2 cut(s) 147, 432
FaeI CATG 6 cut(s) 151, 352, 362, 368, 728, 820
FalI AAGNNNNNCTT 4 cut(s) 173, 205, 441, 473
FatI CATG 6 cut(s) 147, 348, 358, 364, 724, 816
FauI CCCGC 1 cut(s) 271
FbaI TGATCA 2 cut(s) 370, 568
FokI GGATG 3 cut(s) 598, 614, 633
HaeIII GGCC 3 cut(s) 390, 565, 907
HapII CCGG 2 cut(s) 281, 471
HgaI GACGC 2 cut(s) 4, 150
Hin1I GRCGYC 2 cut(s) 15, 161
Hin1II CATG 6 cut(s) 151, 352, 362, 368, 728, 820
HindIII AAGCTT 1 cut(s) 404
HinfI GANTC 5 cut(s) 9, 24, 212, 738, 914
HpaII CCGG 2 cut(s) 281, 471
HphI GGTGA 1 cut(s) 242
Hpy166II GTNNAC 2 cut(s) 286, 653
Hpy188I TCNGA 3 cut(s) 23, 828, 901
Hpy188III TCNNGA 3 cut(s) 205, 397, 725
Hpy8I GTNNAC 2 cut(s) 286, 653
Hpy99I CGWCG 2 cut(s) 32, 166
HpyAV CCTTC 1 cut(s) 202
HpyCH4III ACNGT 3 cut(s) 295, 745, 754
HpyCH4IV ACGT 2 cut(s) 375, 616
HpyCH4V TGCA 3 cut(s) 605, 869, 889
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 866
HpyF3I CTNAG 3 cut(s) 20, 402, 670
HpySE526I ACGT 2 cut(s) 375, 616
Hsp92I GRCGYC 2 cut(s) 15, 161
Hsp92II CATG 6 cut(s) 151, 352, 362, 368, 728, 820
KroI GCCGGC 1 cut(s) 470
KroNI GCCGGC 1 cut(s) 472
Ksp22I TGATCA 2 cut(s) 370, 568
Kzo9I GATC 4 cut(s) 339, 370, 493, 568
LguI GCTCTTC 1 cut(s) 206
LmnI GCTCC 2 cut(s) 47, 189
LweI GCATC 2 cut(s) 592, 756
MaeII ACGT 2 cut(s) 375, 616
MaeIII GTNAC 3 cut(s) 137, 376, 550
MalI GATC 4 cut(s) 341, 372, 495, 570
MbiI CCGCTC 1 cut(s) 266
MboI GATC 4 cut(s) 339, 370, 493, 568
MboII GAAGA 5 cut(s) 54, 165, 168, 181, 193
MflI RGATCY 1 cut(s) 493
MlyI GAGTC 2 cut(s) 221, 923
MroNI GCCGGC 1 cut(s) 470
MseI TTAA 1 cut(s) 807
MslI CAYNNNNRTG 2 cut(s) 363, 860
MspI CCGG 2 cut(s) 281, 471
MspR9I CCNGG 3 cut(s) 281, 411, 522
MvaI CCWGG 2 cut(s) 411, 522
MwoI GCNNNNNNNGC 2 cut(s) 158, 866
NaeI GCCGGC 1 cut(s) 472
NciI CCSGG 1 cut(s) 281
NcoI CCATGG 1 cut(s) 147
NdeII GATC 4 cut(s) 339, 370, 493, 568
NgoMIV GCCGGC 1 cut(s) 470
NlaIII CATG 6 cut(s) 151, 352, 362, 368, 728, 820
NmeAIII GCCGAG 1 cut(s) 760
NmuCI GTSAC 1 cut(s) 376
NspV TTCGAA 1 cut(s) 7
PagI TCATGA 1 cut(s) 724
PceI AGGCCT 1 cut(s) 907
PciSI GCTCTTC 1 cut(s) 206
PdiI GCCGGC 1 cut(s) 472
PfeI GAWTC 3 cut(s) 9, 24, 738
PleI GAGTC 2 cut(s) 220, 922
PpsI GAGTC 2 cut(s) 220, 922
Ppu21I YACGTR 1 cut(s) 617
PsiI TTATAA 1 cut(s) 774
Psp6I CCWGG 2 cut(s) 409, 520
PspGI CCWGG 2 cut(s) 409, 520
PspPI GGNCC 2 cut(s) 454, 563
PstNI CAGNNNCTG 1 cut(s) 866
PsuI RGATCY 1 cut(s) 493
RsaI GTAC 1 cut(s) 751
RsaNI GTAC 1 cut(s) 750
RseI CAYNNNNRTG 2 cut(s) 363, 860
SapI GCTCTTC 1 cut(s) 206
SaqAI TTAA 1 cut(s) 807
Sau3AI GATC 4 cut(s) 339, 370, 493, 568
Sau96I GGNCC 2 cut(s) 454, 563
ScaI AGTACT 1 cut(s) 751
SchI GAGTC 2 cut(s) 221, 923
ScrFI CCNGG 3 cut(s) 281, 411, 522
SfaNI GCATC 2 cut(s) 592, 756
SfuI TTCGAA 1 cut(s) 7
SinI GGWCC 1 cut(s) 454
SmiMI CAYNNNNRTG 2 cut(s) 363, 860
SseBI AGGCCT 1 cut(s) 907
SsiI CCGC 4 cut(s) 118, 193, 264, 452
StuI AGGCCT 1 cut(s) 907
StyD4I CCNGG 3 cut(s) 279, 409, 520
StyI CCWWGG 2 cut(s) 147, 432
TaaI ACNGT 3 cut(s) 295, 745, 754
TaiI ACGT 2 cut(s) 378, 619
TaqI TCGA 3 cut(s) 7, 204, 511
TatI WGTACW 1 cut(s) 749
TfiI GAWTC 3 cut(s) 9, 24, 738
Tru1I TTAA 1 cut(s) 807
Tru9I TTAA 1 cut(s) 807
TscAI CASTG 5 cut(s) 385, 391, 601, 748, 867
TseFI GTSAC 1 cut(s) 376
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 3 cut(s) 353, 365, 665
TspRI CASTG 5 cut(s) 385, 391, 601, 748, 867
VpaK11BI GGWCC 1 cut(s) 454
ZrmI AGTACT 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.