MD17G1135300.v1.1

Persulfide dioxygenase ETHE1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0012170
Physical Location & Seq
Forward (+)
12124274 .. 12128211
3938 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1135300.v1.1.491

Sequence Viewer

Length: 912 bp
ATGCTTCGAATCGGCGTCCTCAGATTCGTCGCTCTCCCTCGCTCCTCTTCCTTTTTCTTCAGTACCACTTCTCCCAAATCCCTCAATCCTTGTACAATTCGCACCAAGTTCGCTTCCCATTCCCGAACGATGGCTTATACGACGTCGTCGGGTTCTTCCTCCAAGCTCCTCTTCCGCCAGCTCTTCGAGAAGGAGTCCTCCACCTACACTTACTTGCTCGCCGATGTCTCTCACCCCGACAAACCCGCTCTGTTGATTGACCCGGTGGACAAAACGGTGGATAGGGATATCTCCCTTGTCAAAGAGTTGGGGCTGAAGTTGATCTACGCCATGAACACTCATGTTCATGCTGATCACGTGACCGGAACCGGTCTCATGAAGACTAAGCTTCCTGGCGTAAAATCTGTCATTTCGAAGGCGAGCAAATCAAAAGCGGACCTTCTTATTGAATCCGGCGATAAAATATATTTTGGCGATCTGTTTTTGGAGGTTAGAGCTACTCCTGGGCATACATTAGGTTGTGTTACGTATGTTACAGGAGATGGGCCTGATCAGCCCCAACCAAGGATGGCTTTTACTGGAGATGCACTACTCATACGTGGATGTGGGAGAACTGATTTTCAGGGTGGCAGTTCACAACAGCTGTACAAGTCAGTTCATTCACAGATTTTTACATTGCCAAAGGATACATATATCTATCCTGCTCATGATTACAAGGGATTCACTGTTAGTACCGTGGGGGAGGAGATGCTCTATAATCCTCGGCTCACAAAAGATGAGGAGGAATTTCAAAGTATCATGGAAAATCTAAAACTCGCATATCCAAAAATGATTGACATAGCTGTACCTGCAAATATGGTTTGTGGATTGCAAGACTTGTCTGAAAGACCTGCCGAGTCCATGGCCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00920 map00920
Pfam Domains
Protein Families
Lactamase_B

Protein Analysis

304

Amino Acids

33.48

Weight (kDa)

8.22

Isoelectric Point (pI)

32.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lactamase_B PF00753 68 - 236 1.9e-11 Metallo-beta-lactamase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53580 AT1G53580
fragaria_vesca FvH4_6g38610
malus_domestica MD09G1149000.v1.1 MD17G1135300.v1.1
prunus_persica Prupe.3G177200_v2.0.a1
pyrus_communis pycom09g06850 pycom17g12930
rosa_chinensis RchiOBHm_Chr2g0152061
rosa_laevigata RLG00000020603
rosa_multiflora Rmu_sc0000760.1_g000022
rosa_roxburghii Rroxscaffold_2G00096020
rosa_rugosa Rorug02G0430500 Rorug02G0430500
rosa_samantha Rh2AG491300 Rh2BG503700 Rh2CG477700 Rh2DG514900
rosa_wichuraiana Rw2G040390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 146
Acc36I ACCTGC 2 cut(s) 856, 898
AccBSI CCGCTC 1 cut(s) 248
AciI CCGC 3 cut(s) 175, 246, 434
AcoI YGGCCR 1 cut(s) 903
AcsI RAATTY 1 cut(s) 785
AcuI CTGAAG 2 cut(s) 43, 335
AcvI CACGTG 1 cut(s) 358
AcyI GRCGYC 2 cut(s) 15, 143
AfaI GTAC 5 cut(s) 64, 94, 647, 733, 846
AfiI CCNNNNNNNGG 2 cut(s) 130, 564
AgeI ACCGGT 1 cut(s) 368
AgsI TTSAA 2 cut(s) 449, 791
AjnI CCWGG 2 cut(s) 391, 502
AjuI GAANNNNNNNTTGG 2 cut(s) 155, 187
AluBI AGCT 6 cut(s) 166, 181, 388, 497, 643, 842
AluI AGCT 6 cut(s) 166, 181, 388, 497, 643, 842
Alw26I GTCTC 2 cut(s) 232, 377
AoxI GGCC 2 cut(s) 545, 903
ApoI RAATTY 1 cut(s) 785
AsiGI ACCGGT 1 cut(s) 368
AspS9I GGNCC 2 cut(s) 436, 545
AsuC2I CCSGG 1 cut(s) 263
AsuHPI GGTGA 1 cut(s) 224
AsuII TTCGAA 2 cut(s) 7, 413
AvaII GGWCC 1 cut(s) 436
BalI TGGCCA 1 cut(s) 905
BarI GAAGNNNNNNTAC 2 cut(s) 308, 340
BbrPI CACGTG 1 cut(s) 358
BbsI GAAGAC 1 cut(s) 386
BccI CCATC 3 cut(s) 124, 536, 562
BcgI CGANNNNNNTGC 2 cut(s) 91, 125
BciT130I CCWGG 2 cut(s) 393, 504
BciVI GTATCC 1 cut(s) 679
BclI TGATCA 2 cut(s) 352, 550
BcnI CCSGG 1 cut(s) 263
BcoDI GTCTC 2 cut(s) 232, 377
BfaI CTAG 1 cut(s) 910
BfuAI ACCTGC 2 cut(s) 856, 898
BfuI GTATCC 1 cut(s) 679
Bme1390I CCNGG 3 cut(s) 263, 393, 504
Bme18I GGWCC 1 cut(s) 436
BmgT120I GGNCC 2 cut(s) 436, 545
BmiI GGNNCC 1 cut(s) 367
BmrFI CCNGG 3 cut(s) 263, 393, 504
BmsI GCATC 2 cut(s) 574, 738
BpiI GAAGAC 1 cut(s) 386
BpmI CTGGAG 1 cut(s) 600
Bpu14I TTCGAA 2 cut(s) 7, 413
BpuMI CCSGG 1 cut(s) 263
BsaAI YACGTR 3 cut(s) 358, 528, 599
BsaHI GRCGYC 2 cut(s) 15, 143
BsaI GGTCTC 1 cut(s) 377
BsaJI CCNNGG 5 cut(s) 503, 563, 735, 761, 900
BsaWI WCCGGW 2 cut(s) 362, 368
BsaXI ACNNNNNCTCC 2 cut(s) 55, 85
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 564
Bse118I RCCGGY 1 cut(s) 368
Bse1I ACTGG 1 cut(s) 583
Bse3DI GCAATG 1 cut(s) 674
BseBI CCWGG 2 cut(s) 393, 504
BseDI CCNNGG 5 cut(s) 503, 563, 735, 761, 900
BseGI GGATG 2 cut(s) 573, 608
BseLI CCNNNNNNNGG 2 cut(s) 130, 564
BseMI GCAATG 1 cut(s) 674
BseMII CTCAG 1 cut(s) 34
BseNI ACTGG 1 cut(s) 583
BseRI GAGGAG 4 cut(s) 34, 158, 758, 794
BshFI GGCC 2 cut(s) 547, 905
BshTI ACCGGT 1 cut(s) 368
BsiSI CCGG 4 cut(s) 263, 363, 369, 453
BslI CCNNNNNNNGG 2 cut(s) 130, 564
BsmAI GTCTC 2 cut(s) 232, 377
BsnI GGCC 2 cut(s) 547, 905
Bso31I GGTCTC 1 cut(s) 377
Bsp119I TTCGAA 2 cut(s) 7, 413
Bsp1407I TGTACA 2 cut(s) 92, 645
Bsp143I GATC 4 cut(s) 321, 352, 475, 550
Bsp19I CCATGG 1 cut(s) 900
BspACI CCGC 3 cut(s) 175, 246, 434
BspANI GGCC 2 cut(s) 547, 905
BspCNI CTCAG 1 cut(s) 33
BspHI TCATGA 2 cut(s) 375, 706
BspLI GGNNCC 1 cut(s) 367
BspMI ACCTGC 2 cut(s) 856, 898
BspQI GCTCTTC 1 cut(s) 188
BspT104I TTCGAA 2 cut(s) 7, 413
BspTNI GGTCTC 1 cut(s) 377
BsrBI CCGCTC 1 cut(s) 248
BsrDI GCAATG 1 cut(s) 674
BsrFI RCCGGY 1 cut(s) 368
BsrGI TGTACA 2 cut(s) 92, 645
BsrI ACTGG 1 cut(s) 583
BssAI RCCGGY 1 cut(s) 368
BssECI CCNNGG 5 cut(s) 503, 563, 735, 761, 900
BssMI GATC 4 cut(s) 321, 352, 475, 550
BssNI GRCGYC 2 cut(s) 15, 143
BssT1I CCWWGG 2 cut(s) 563, 900
Bst2UI CCWGG 2 cut(s) 393, 504
Bst4CI ACNGT 3 cut(s) 277, 727, 736
Bst6I CTCTTC 3 cut(s) 52, 176, 188
BstACI GRCGYC 2 cut(s) 15, 143
BstAUI TGTACA 2 cut(s) 92, 645
BstBAI YACGTR 3 cut(s) 358, 528, 599
BstBI TTCGAA 2 cut(s) 7, 413
BstC8I GCNNGC 3 cut(s) 179, 219, 421
BstDEI CTNAG 2 cut(s) 20, 384
BstDSI CCRYGG 2 cut(s) 735, 900
BstF5I GGATG 2 cut(s) 573, 608
BstKTI GATC 4 cut(s) 324, 355, 478, 553
BstMAI GTCTC 2 cut(s) 232, 377
BstMBI GATC 4 cut(s) 321, 352, 475, 550
BstMWI GCNNNNNNNGC 2 cut(s) 553, 848
BstNI CCWGG 2 cut(s) 393, 504
BstSCI CCNGG 3 cut(s) 261, 391, 502
BstSNI TACGTA 1 cut(s) 528
BstV2I GAAGAC 1 cut(s) 386
BsuI GTATCC 1 cut(s) 679
BsuRI GGCC 2 cut(s) 547, 905
BtgI CCRYGG 2 cut(s) 735, 900
BtsCI GGATG 2 cut(s) 573, 608
BtsIMutI CAGTG 1 cut(s) 723
BveI ACCTGC 2 cut(s) 856, 898
Cac8I GCNNGC 3 cut(s) 179, 219, 421
CciI TCATGA 2 cut(s) 375, 706
Cfr10I RCCGGY 1 cut(s) 368
Cfr13I GGNCC 2 cut(s) 436, 545
CseI GACGC 1 cut(s) 4
Csp6I GTAC 5 cut(s) 63, 93, 646, 732, 845
CspAI ACCGGT 1 cut(s) 368
CviAII CATG 7 cut(s) 331, 341, 347, 376, 707, 799, 901
CviQI GTAC 5 cut(s) 63, 93, 646, 732, 845
DdeI CTNAG 2 cut(s) 20, 384
DpnI GATC 4 cut(s) 323, 354, 477, 552
DpnII GATC 4 cut(s) 321, 352, 475, 550
EaeI YGGCCR 1 cut(s) 903
Eam1104I CTCTTC 3 cut(s) 52, 176, 188
EarI CTCTTC 3 cut(s) 52, 176, 188
EciI GGCGGA 1 cut(s) 164
Eco105I TACGTA 1 cut(s) 528
Eco130I CCWWGG 2 cut(s) 563, 900
Eco31I GGTCTC 1 cut(s) 377
Eco32I GATATC 1 cut(s) 289
Eco47I GGWCC 1 cut(s) 436
Eco57I CTGAAG 2 cut(s) 43, 335
Eco72I CACGTG 1 cut(s) 358
EcoRII CCWGG 2 cut(s) 391, 502
EcoRV GATATC 1 cut(s) 289
EcoT14I CCWWGG 2 cut(s) 563, 900
ErhI CCWWGG 2 cut(s) 563, 900
FaeI CATG 7 cut(s) 334, 344, 350, 379, 710, 802, 904
FalI AAGNNNNNCTT 6 cut(s) 155, 187, 423, 455, 556, 588
FatI CATG 7 cut(s) 330, 340, 346, 375, 706, 798, 900
FauI CCCGC 1 cut(s) 253
FbaI TGATCA 2 cut(s) 352, 550
FokI GGATG 2 cut(s) 580, 615
FspBI CTAG 1 cut(s) 910
GsuI CTGGAG 1 cut(s) 600
HaeIII GGCC 2 cut(s) 547, 905
HapII CCGG 4 cut(s) 263, 363, 369, 453
HgaI GACGC 1 cut(s) 4
Hin1I GRCGYC 2 cut(s) 15, 143
Hin1II CATG 7 cut(s) 334, 344, 350, 379, 710, 802, 904
HindIII AAGCTT 1 cut(s) 386
HinfI GANTC 6 cut(s) 9, 24, 194, 449, 720, 896
HpaII CCGG 4 cut(s) 263, 363, 369, 453
HphI GGTGA 1 cut(s) 224
Hpy166II GTNNAC 2 cut(s) 268, 635
Hpy188I TCNGA 2 cut(s) 23, 883
Hpy188III TCNNGA 4 cut(s) 123, 187, 376, 707
Hpy8I GTNNAC 2 cut(s) 268, 635
Hpy99I CGWCG 4 cut(s) 32, 145, 148, 151
HpyAV CCTTC 3 cut(s) 184, 409, 449
HpyCH4III ACNGT 3 cut(s) 277, 727, 736
HpyCH4IV ACGT 4 cut(s) 143, 357, 527, 598
HpyCH4V TGCA 3 cut(s) 587, 851, 871
HpyF10VI GCNNNNNNNGC 2 cut(s) 553, 848
HpyF3I CTNAG 2 cut(s) 20, 384
HpySE526I ACGT 4 cut(s) 143, 357, 527, 598
Hsp92I GRCGYC 2 cut(s) 15, 143
Hsp92II CATG 7 cut(s) 334, 344, 350, 379, 710, 802, 904
Ksp22I TGATCA 2 cut(s) 352, 550
Kzo9I GATC 4 cut(s) 321, 352, 475, 550
LguI GCTCTTC 1 cut(s) 188
LmnI GCTCC 2 cut(s) 47, 171
LweI GCATC 2 cut(s) 574, 738
MaeI CTAG 1 cut(s) 910
MaeII ACGT 4 cut(s) 143, 357, 527, 598
MaeIII GTNAC 3 cut(s) 358, 523, 532
MalI GATC 4 cut(s) 323, 354, 477, 552
MbiI CCGCTC 1 cut(s) 248
MboI GATC 4 cut(s) 321, 352, 475, 550
MboII GAAGA 6 cut(s) 39, 49, 147, 163, 175, 391
MlsI TGGCCA 1 cut(s) 905
MluCI AATT 2 cut(s) 96, 785
MluNI TGGCCA 1 cut(s) 905
MlyI GAGTC 2 cut(s) 203, 905
Mox20I TGGCCA 1 cut(s) 905
MscI TGGCCA 1 cut(s) 905
MslI CAYNNNNRTG 1 cut(s) 345
Msp20I TGGCCA 1 cut(s) 905
MspA1I CMGCKG 1 cut(s) 643
MspI CCGG 4 cut(s) 263, 363, 369, 453
MspR9I CCNGG 3 cut(s) 263, 393, 504
MvaI CCWGG 2 cut(s) 393, 504
MwoI GCNNNNNNNGC 2 cut(s) 553, 848
NciI CCSGG 1 cut(s) 263
NcoI CCATGG 1 cut(s) 900
NdeII GATC 4 cut(s) 321, 352, 475, 550
NlaIII CATG 7 cut(s) 334, 344, 350, 379, 710, 802, 904
NlaIV GGNNCC 1 cut(s) 367
NmeAIII GCCGAG 1 cut(s) 742
NmuCI GTSAC 1 cut(s) 358
NspV TTCGAA 2 cut(s) 7, 413
PagI TCATGA 2 cut(s) 375, 706
PciSI GCTCTTC 1 cut(s) 188
PfeI GAWTC 4 cut(s) 9, 24, 449, 720
PflFI GACNNNGTC 1 cut(s) 145
PinAI ACCGGT 1 cut(s) 368
PleI GAGTC 2 cut(s) 202, 904
PmaCI CACGTG 1 cut(s) 358
PmlI CACGTG 1 cut(s) 358
PpsI GAGTC 2 cut(s) 202, 904
Ppu21I YACGTR 3 cut(s) 358, 528, 599
Psp6I CCWGG 2 cut(s) 391, 502
PspCI CACGTG 1 cut(s) 358
PspGI CCWGG 2 cut(s) 391, 502
PspN4I GGNNCC 1 cut(s) 367
PspPI GGNCC 2 cut(s) 436, 545
PsyI GACNNNGTC 1 cut(s) 145
PvuII CAGCTG 1 cut(s) 643
RsaI GTAC 5 cut(s) 64, 94, 647, 733, 846
RsaNI GTAC 5 cut(s) 63, 93, 646, 732, 845
RseI CAYNNNNRTG 1 cut(s) 345
SapI GCTCTTC 1 cut(s) 188
Sau3AI GATC 4 cut(s) 321, 352, 475, 550
Sau96I GGNCC 2 cut(s) 436, 545
SchI GAGTC 2 cut(s) 203, 905
ScrFI CCNGG 3 cut(s) 263, 393, 504
SfaNI GCATC 2 cut(s) 574, 738
SfuI TTCGAA 2 cut(s) 7, 413
SinI GGWCC 1 cut(s) 436
SmiMI CAYNNNNRTG 1 cut(s) 345
SnaBI TACGTA 1 cut(s) 528
Sse9I AATT 2 cut(s) 96, 785
SsiI CCGC 3 cut(s) 175, 246, 434
SspMI CTAG 1 cut(s) 910
StyD4I CCNGG 3 cut(s) 261, 391, 502
StyI CCWWGG 2 cut(s) 563, 900
TaaI ACNGT 3 cut(s) 277, 727, 736
TaiI ACGT 4 cut(s) 146, 360, 530, 601
TaqI TCGA 3 cut(s) 7, 186, 413
TasI AATT 2 cut(s) 96, 785
TatI WGTACW 2 cut(s) 92, 645
TfiI GAWTC 4 cut(s) 9, 24, 449, 720
TscAI CASTG 1 cut(s) 730
TseFI GTSAC 1 cut(s) 358
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 4 cut(s) 335, 347, 392, 647
TspRI CASTG 1 cut(s) 730
Tth111I GACNNNGTC 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 436
XapI RAATTY 1 cut(s) 785
XspI CTAG 1 cut(s) 910
ZraI GACGTC 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.