MD09G1151900.v1.1

mitochondrion organization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012364
Physical Location & Seq
Forward (+)
12040029 .. 12041614
1586 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1151900.v1.1.491

Sequence Viewer

Length: 738 bp
ATGGAGTTAGAGATTGAACGGACCGAGGAGGGGAAAGATGTGCTGGAATCGGTTGCGAATAGAGCGATGGAGCTTGAGACTGAGGTAGCGAGGCTCCAACACGATCTGATCTCTGCCATGGCAGAAGGGGAGGATGCAAATAACGAAGTGGCGGAGCTGAAGCGTGTGTTGGGCGAGAGGGGGGAGAAAATTGATAGCTTGGAGAAGGAGCTCGAGAGCCTGAAGAAGGCGAAGGCTGATAGTGAGAAGAGGATTAGGGAATTGGAGAGGAAGATTGGGGTTTTGGAAGTGAAGGAGACTGAGGAGAAGAGCAAGAAAATTAGGGTTGAGGAGGAGATGAGAGAGAGACTTGATGAGAAAGAGAGTGAGCTTAGCTTGTTCAAGAAGAAAGTGGAGGATTTGGAGTCGGTGATTGTGAAGAACAATGCTGAATTGGGGAAGAGAGTGAGTGAGAAGCTCAATGTCGAGGCGGCGCTGAGGGAATCAGAGGATAAGTGCAGAGGCATGGAAATGAAGTTGGGAAAATTGCAGAAGGACGTGCTGGATGCTGAAAAGGTTATTAATGGATTGAAGGAGAGGACTGTTGGGGAAATTAATGGGACTGTGAATGAAGTGAAGGAGATTTCTAGAGAGACAGAGTCGAAAGGATTGAGCTTGCCAGTTGTGGCAGGGTCCACTGGAGTCGTTGTTGCCACAGCAGCTGCTGTTGTCTATGTGTTGTATGTAAGGCAGAGGTGA

Protein Analysis

246

Amino Acids

27.59

Weight (kDa)

5.06

Isoelectric Point (pI)

33.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC39 PF24161 18 - 190 6.9e-08 Coiled-coil domain-containing protein 39
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 152, 470
AcuI CTGAAG 2 cut(s) 179, 242
AfiI CCNNNNNNNGG 2 cut(s) 30, 226
AgsI TTSAA 3 cut(s) 17, 382, 571
AjiI CACGTC 1 cut(s) 538
AjuI GAANNNNNNNTTGG 2 cut(s) 152, 184
AluBI AGCT 9 cut(s) 73, 157, 198, 211, 370, 375, 457, 654, 701
AluI AGCT 9 cut(s) 73, 157, 198, 211, 370, 375, 457, 654, 701
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 4 cut(s) 71, 290, 340, 626
AlwNI CAGNNNCTG 2 cut(s) 701, 704
Ama87I CYCGRG 1 cut(s) 212
ApeKI GCWGC 2 cut(s) 698, 701
AseI ATTAAT 2 cut(s) 561, 594
AspLEI GCGC 1 cut(s) 475
AspS9I GGNCC 2 cut(s) 21, 672
AsuHPI GGTGA 1 cut(s) 421
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 2 cut(s) 21, 672
BanII GRGCYC 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 213
BbvCI CCTCAGC 1 cut(s) 476
BbvI GCAGC 2 cut(s) 688, 710
BccI CCATC 1 cut(s) 61
BcoDI GTCTC 4 cut(s) 71, 290, 340, 626
BfaI CTAG 1 cut(s) 627
BfoI RGCGCY 1 cut(s) 476
BisI GCNGC 3 cut(s) 471, 699, 702
BlpI GCTNAGC 1 cut(s) 371
BlsI GCNGC 3 cut(s) 472, 700, 703
Bme18I GGWCC 2 cut(s) 21, 672
BmeT110I CYCGRG 1 cut(s) 212
BmgBI CACGTC 1 cut(s) 538
BmgT120I GGNCC 2 cut(s) 21, 672
BmiI GGNNCC 2 cut(s) 95, 673
BmsI GCATC 2 cut(s) 124, 535
BpmI CTGGAG 1 cut(s) 699
Bpu10I CCTNAGC 1 cut(s) 476
Bpu1102I GCTNAGC 1 cut(s) 371
BpuEI CTTGAG 1 cut(s) 95
BsaJI CCNNGG 2 cut(s) 24, 117
BsaXI ACNNNNNCTCC 2 cut(s) 672, 702
Bsc4I CCNNNNNNNGG 2 cut(s) 30, 226
Bse1I ACTGG 2 cut(s) 659, 682
BseDI CCNNGG 2 cut(s) 24, 117
BseGI GGATG 2 cut(s) 139, 550
BseLI CCNNNNNNNGG 2 cut(s) 30, 226
BseMII CTCAG 3 cut(s) 72, 291, 467
BseNI ACTGG 2 cut(s) 659, 682
BseRI GAGGAG 4 cut(s) 41, 317, 344, 347
BseXI GCAGC 2 cut(s) 688, 710
BsgI GTGCAG 1 cut(s) 517
BsiHKAI GWGCWC 1 cut(s) 213
BsiHKCI CYCGRG 1 cut(s) 212
BslFI GGGAC 1 cut(s) 613
BslI CCNNNNNNNGG 2 cut(s) 30, 226
BsmAI GTCTC 4 cut(s) 71, 290, 340, 626
BsmFI GGGAC 1 cut(s) 613
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 2 cut(s) 103, 108
Bsp1720I GCTNAGC 1 cut(s) 371
Bsp19I CCATGG 1 cut(s) 117
BspACI CCGC 2 cut(s) 152, 470
BspCNI CTCAG 3 cut(s) 73, 292, 468
BspLI GGNNCC 2 cut(s) 95, 673
BspQI GCTCTTC 1 cut(s) 302
BsrI ACTGG 2 cut(s) 659, 682
BssECI CCNNGG 2 cut(s) 24, 117
BssMI GATC 2 cut(s) 103, 108
BssT1I CCWWGG 1 cut(s) 117
Bst4CI ACNGT 2 cut(s) 583, 604
Bst6I CTCTTC 3 cut(s) 242, 302, 434
BstC8I GCNNGC 1 cut(s) 656
BstDEI CTNAG 4 cut(s) 81, 300, 371, 476
BstDSI CCRYGG 1 cut(s) 117
BstENI CCTNNNNNAGG 1 cut(s) 224
BstF5I GGATG 2 cut(s) 139, 550
BstH2I RGCGCY 1 cut(s) 476
BstHHI GCGC 1 cut(s) 475
BstKTI GATC 2 cut(s) 106, 111
BstMAI GTCTC 4 cut(s) 71, 290, 340, 626
BstMBI GATC 2 cut(s) 103, 108
BstMWI GCNNNNNNNGC 2 cut(s) 62, 698
BstV1I GCAGC 2 cut(s) 688, 710
BtgI CCRYGG 1 cut(s) 117
BtgZI GCGATG 1 cut(s) 80
BtrI CACGTC 1 cut(s) 538
BtsCI GGATG 2 cut(s) 139, 550
BtsIMutI CAGTG 1 cut(s) 675
Cac8I GCNNGC 1 cut(s) 656
CaiI CAGNNNCTG 2 cut(s) 701, 704
CfoI GCGC 1 cut(s) 475
Cfr13I GGNCC 2 cut(s) 21, 672
CpoI CGGWCCG 1 cut(s) 21
CspI CGGWCCG 1 cut(s) 21
CviAII CATG 2 cut(s) 118, 505
DdeI CTNAG 4 cut(s) 81, 300, 371, 476
DpnI GATC 2 cut(s) 105, 110
DpnII GATC 2 cut(s) 103, 108
Eam1104I CTCTTC 3 cut(s) 242, 302, 434
EarI CTCTTC 3 cut(s) 242, 302, 434
EciI GGCGGA 1 cut(s) 167
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 117
Eco24I GRGCYC 1 cut(s) 213
Eco47I GGWCC 2 cut(s) 21, 672
Eco53kI GAGCTC 1 cut(s) 211
Eco57I CTGAAG 2 cut(s) 179, 242
Eco88I CYCGRG 1 cut(s) 212
EcoICRI GAGCTC 1 cut(s) 211
EcoNI CCTNNNNNAGG 1 cut(s) 224
EcoT14I CCWWGG 1 cut(s) 117
EcoT38I GRGCYC 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 117
FaeI CATG 2 cut(s) 121, 508
FaiI YATR 4 cut(s) 119, 506, 714, 723
FaqI GGGAC 1 cut(s) 613
FatI CATG 2 cut(s) 117, 504
Fnu4HI GCNGC 3 cut(s) 471, 699, 702
FokI GGATG 2 cut(s) 146, 557
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 3 cut(s) 471, 699, 702
FspBI CTAG 1 cut(s) 627
GlaI GCGC 1 cut(s) 474
GluI GCNGC 3 cut(s) 471, 699, 702
GsuI CTGGAG 1 cut(s) 699
HaeII RGCGCY 1 cut(s) 476
HhaI GCGC 1 cut(s) 475
Hin1II CATG 2 cut(s) 121, 508
Hin6I GCGC 1 cut(s) 473
HinP1I GCGC 1 cut(s) 473
HinfI GANTC 5 cut(s) 47, 404, 482, 638, 681
HphI GGTGA 1 cut(s) 421
Hpy166II GTNNAC 1 cut(s) 675
Hpy188I TCNGA 2 cut(s) 108, 487
Hpy188III TCNNGA 3 cut(s) 214, 382, 627
Hpy8I GTNNAC 1 cut(s) 675
HpyAV CCTTC 8 cut(s) 119, 199, 220, 226, 286, 526, 565, 610
HpyCH4III ACNGT 2 cut(s) 583, 604
HpyCH4IV ACGT 1 cut(s) 537
HpyCH4V TGCA 3 cut(s) 137, 498, 529
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 698
HpyF3I CTNAG 4 cut(s) 81, 300, 371, 476
HpySE526I ACGT 1 cut(s) 537
Hsp92II CATG 2 cut(s) 121, 508
HspAI GCGC 1 cut(s) 473
Kzo9I GATC 2 cut(s) 103, 108
LguI GCTCTTC 1 cut(s) 302
LmnI GCTCC 4 cut(s) 70, 99, 154, 208
LpnPI CCDG 6 cut(s) 29, 233, 527, 654, 663, 672
Lsp1109I GCAGC 2 cut(s) 688, 710
LweI GCATC 2 cut(s) 124, 535
MaeI CTAG 1 cut(s) 627
MaeII ACGT 1 cut(s) 537
MalI GATC 2 cut(s) 105, 110
MboI GATC 2 cut(s) 103, 108
MboII GAAGA 7 cut(s) 235, 259, 283, 319, 397, 430, 451
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 6 cut(s) 189, 260, 318, 431, 524, 591
MlyI GAGTC 3 cut(s) 413, 647, 690
MmeI TCCRAC 1 cut(s) 121
MseI TTAA 2 cut(s) 561, 594
MslI CAYNNNNRTG 1 cut(s) 509
MspA1I CMGCKG 1 cut(s) 701
MwoI GCNNNNNNNGC 2 cut(s) 62, 698
NcoI CCATGG 1 cut(s) 117
NdeII GATC 2 cut(s) 103, 108
NlaIII CATG 2 cut(s) 121, 508
NlaIV GGNNCC 2 cut(s) 95, 673
PaeR7I CTCGAG 1 cut(s) 212
PciSI GCTCTTC 1 cut(s) 302
PfeI GAWTC 2 cut(s) 47, 482
PflFI GACNNNGTC 1 cut(s) 637
PkrI GCNGC 3 cut(s) 472, 700, 703
PleI GAGTC 3 cut(s) 412, 646, 689
PpsI GAGTC 3 cut(s) 412, 646, 689
PshBI ATTAAT 2 cut(s) 561, 594
Psp124BI GAGCTC 1 cut(s) 213
PspN4I GGNNCC 2 cut(s) 95, 673
PspPI GGNCC 2 cut(s) 21, 672
PstNI CAGNNNCTG 2 cut(s) 701, 704
PsyI GACNNNGTC 1 cut(s) 637
PvuII CAGCTG 1 cut(s) 701
RseI CAYNNNNRTG 1 cut(s) 509
Rsr2I CGGWCCG 1 cut(s) 21
RsrII CGGWCCG 1 cut(s) 21
SacI GAGCTC 1 cut(s) 213
SapI GCTCTTC 1 cut(s) 302
SaqAI TTAA 2 cut(s) 561, 594
SatI GCNGC 3 cut(s) 471, 699, 702
Sau3AI GATC 2 cut(s) 103, 108
Sau96I GGNCC 2 cut(s) 21, 672
SchI GAGTC 3 cut(s) 413, 647, 690
SduI GDGCHC 1 cut(s) 213
SfaNI GCATC 2 cut(s) 124, 535
Sfr274I CTCGAG 1 cut(s) 212
SinI GGWCC 2 cut(s) 21, 672
SlaI CTCGAG 1 cut(s) 212
SmiMI CAYNNNNRTG 1 cut(s) 509
SmlI CTYRAG 2 cut(s) 74, 212
SmoI CTYRAG 2 cut(s) 74, 212
Sse9I AATT 6 cut(s) 189, 260, 318, 431, 524, 591
SsiI CCGC 2 cut(s) 152, 470
SspMI CTAG 1 cut(s) 627
SstI GAGCTC 1 cut(s) 213
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 2 cut(s) 583, 604
TaiI ACGT 1 cut(s) 540
TaqI TCGA 3 cut(s) 213, 465, 641
TaqII GACCGA 1 cut(s) 38
TasI AATT 6 cut(s) 189, 260, 318, 431, 524, 591
TauI GCSGC 1 cut(s) 473
TfiI GAWTC 2 cut(s) 47, 482
Tru1I TTAA 2 cut(s) 561, 594
Tru9I TTAA 2 cut(s) 561, 594
TscAI CASTG 1 cut(s) 682
TseI GCWGC 2 cut(s) 698, 701
TspDTI ATGAA 2 cut(s) 527, 624
TspGWI ACGGA 1 cut(s) 34
TspRI CASTG 1 cut(s) 682
Tth111I GACNNNGTC 1 cut(s) 637
VpaK11BI GGWCC 2 cut(s) 21, 672
VspI ATTAAT 2 cut(s) 561, 594
XagI CCTNNNNNAGG 1 cut(s) 224
XbaI TCTAGA 1 cut(s) 626
XhoI CTCGAG 1 cut(s) 212
XspI CTAG 1 cut(s) 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.