MD09G1169400.v1.1

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0009746
Physical Location & Seq
Forward (+)
14072720 .. 14073777
1058 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1169400.v1.1.491

Sequence Viewer

Length: 894 bp
ATGTCAGCCTGGGCTTCACGAGTGGAGGGAAAAAGGGTAGCGGATTTAGTTGAGGGTCGCTCTTTCTGGACTGGAGCTGAGATGGTCCTGAACGCAGCTATGCCTCTCATTGGCATTCTAAACTGGATCTTTGAGGCTGACGAGCCGCTTATTGGGTACATATATGAAACAATGGATCAAGTAAAGGAGACAATTAAGGAAGAGTTCAACAAAAAGAAACCAGATTACATGCCAATCTGGGAAGTTGTTGATGAGATATGGAACAATGTTCTCCACAGTCCTCTCCACGCGGCTGGCTATTATCTTAACCCGAGTCTCTTCTATTCAAGTGACTTTTATCCTGACTTCGAGGTTTCTTCTGGCCTTCTGAGCTGCCTTGGACAACTGGTCCAGAGTCAGCCAATCAAAGACTTAATTATCCGTCAATTGGAGGAGTATGCACACGGCAAAGGTAGTTATCAGGAGGGGAGTTCCAGAACTCGAAGAAGAAACGTTCCACCAGCTATGTGGTGGTCTAGTTATGGAGAAAATCATCCTGAACTGCAGAGGTTTGCGGTTCGAATTTTGAGTCAGAACTGCGATGGCGCTTTAAGATATGTGCTCAAACGGACTATGGCGGAGAAACTGCTAACAAATGGAAGGAATCCTGTTGAGCAACAGCGGCTTAAATATCTTACATTTGTTCATTATAATTTGCAGTTACAGCAGTTTCATGCAGGTATGAAGAGCGGAGTTGAGGCTGAGGAGATTGATCCAATGGATGATTGGATCATTGATGAAGCACCAGACGTTGTGCCCCAAAATCTTGAATCGTCGTGGATGAACTTGGACTGTGCTAGAGCTCTCAGCGAGGAAGGTTGTTCCAGGTTTGCTGCAAAGACGGAAACCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

298

Amino Acids

34.11

Weight (kDa)

4.99

Isoelectric Point (pI)

55.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009746)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13010 AT3G13020 AT3G13030 AT3G13030 AT3G13030 AT3G13030 AT3G13030
fragaria_vesca FvH4_6g36422
malus_domestica MD09G1169400.v1.1
pyrus_communis pycom09g08680
rosa_chinensis RchiOBHm_Chr2g0148401
rosa_laevigata RLG00000020348
rosa_roxburghii Rroxscaffold_2G00098760
rosa_rugosa Rorug02G0406800
rosa_samantha Rh2AG465500 Rh2BG478400 Rh2BG478500 Rh2CG452000 Rh2DG487200 Rh2DG487300
rosa_wichuraiana Rw2G037940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 690
Acc36I ACCTGC 1 cut(s) 707
AccBSI CCGCTC 1 cut(s) 729
AccII CGCG 1 cut(s) 290
AciI CCGC 7 cut(s) 41, 146, 290, 554, 617, 661, 729
AclI AACGTT 1 cut(s) 492
AclWI GGATC 4 cut(s) 134, 183, 746, 776
AcsI RAATTY 1 cut(s) 561
AfaI GTAC 1 cut(s) 158
AfiI CCNNNNNNNGG 3 cut(s) 110, 152, 427
AgsI TTSAA 3 cut(s) 208, 327, 809
AhdI GACNNNNNGTC 1 cut(s) 386
AjnI CCWGG 2 cut(s) 8, 863
AluBI AGCT 5 cut(s) 77, 98, 372, 503, 842
AluI AGCT 5 cut(s) 77, 98, 372, 503, 842
Alw21I GWGCWC 2 cut(s) 603, 844
Alw26I GTCTC 2 cut(s) 182, 320
AlwI GGATC 4 cut(s) 134, 183, 746, 776
Ama87I CYCGRG 1 cut(s) 310
AoxI GGCC 1 cut(s) 361
ApeKI GCWGC 3 cut(s) 95, 372, 872
ApoI RAATTY 1 cut(s) 561
ArsI GACNNNNNNTTYG 2 cut(s) 553, 585
AspLEI GCGC 1 cut(s) 587
AspS9I GGNCC 2 cut(s) 85, 388
AsuII TTCGAA 1 cut(s) 559
AvaI CYCGRG 1 cut(s) 310
AvaII GGWCC 2 cut(s) 85, 388
BaeGI GKGCMC 1 cut(s) 798
BanII GRGCYC 1 cut(s) 844
BauI CACGAG 1 cut(s) 18
Bbv12I GWGCWC 2 cut(s) 603, 844
BbvCI CCTCAGC 1 cut(s) 741
BbvI GCAGC 3 cut(s) 107, 359, 859
BccI CCATC 2 cut(s) 76, 575
BceAI ACGGC 1 cut(s) 460
BciT130I CCWGG 2 cut(s) 10, 865
BcoDI GTCTC 2 cut(s) 182, 320
BfaI CTAG 2 cut(s) 516, 837
BfmI CTRYAG 1 cut(s) 542
BfoI RGCGCY 1 cut(s) 588
BfuAI ACCTGC 1 cut(s) 707
BisI GCNGC 6 cut(s) 96, 146, 291, 373, 662, 873
BlsI GCNGC 6 cut(s) 97, 147, 292, 374, 663, 874
Bme1390I CCNGG 2 cut(s) 10, 865
Bme18I GGWCC 2 cut(s) 85, 388
BmeRI GACNNNNNGTC 1 cut(s) 386
BmeT110I CYCGRG 1 cut(s) 310
BmgT120I GGNCC 2 cut(s) 85, 388
BmrFI CCNGG 2 cut(s) 10, 865
BplI GAGNNNNNCTC 2 cut(s) 44, 76
BpmI CTGGAG 1 cut(s) 93
Bpu10I CCTNAGC 1 cut(s) 741
Bpu14I TTCGAA 1 cut(s) 559
BsaJI CCNNGG 2 cut(s) 9, 376
Bsc4I CCNNNNNNNGG 3 cut(s) 110, 152, 427
Bse1I ACTGG 3 cut(s) 76, 128, 390
BseBI CCWGG 2 cut(s) 10, 865
BseDI CCNNGG 2 cut(s) 9, 376
BseGI GGATG 3 cut(s) 532, 766, 825
BseLI CCNNNNNNNGG 3 cut(s) 110, 152, 427
BseMII CTCAG 4 cut(s) 69, 359, 732, 859
BseNI ACTGG 3 cut(s) 76, 128, 390
BseRI GAGGAG 2 cut(s) 446, 758
BseSI GKGCMC 1 cut(s) 798
BseXI GCAGC 3 cut(s) 107, 359, 859
Bsh1236I CGCG 1 cut(s) 290
BshFI GGCC 1 cut(s) 363
BsiHKAI GWGCWC 2 cut(s) 603, 844
BsiHKCI CYCGRG 1 cut(s) 310
BslI CCNNNNNNNGG 3 cut(s) 110, 152, 427
BsmAI GTCTC 2 cut(s) 182, 320
BsmI GAATGC 1 cut(s) 114
BsnI GGCC 1 cut(s) 363
BsoBI CYCGRG 1 cut(s) 310
Bsp119I TTCGAA 1 cut(s) 559
Bsp1286I GDGCHC 3 cut(s) 603, 798, 844
Bsp143I GATC 4 cut(s) 126, 175, 751, 768
BspACI CCGC 7 cut(s) 41, 146, 290, 554, 617, 661, 729
BspANI GGCC 1 cut(s) 363
BspCNI CTCAG 4 cut(s) 70, 360, 733, 858
BspFNI CGCG 1 cut(s) 290
BspMAI CTGCAG 1 cut(s) 546
BspMI ACCTGC 1 cut(s) 707
BspPI GGATC 4 cut(s) 134, 183, 746, 776
BspQI GCTCTTC 1 cut(s) 719
BspT104I TTCGAA 1 cut(s) 559
BsrBI CCGCTC 1 cut(s) 729
BsrI ACTGG 3 cut(s) 76, 128, 390
BssECI CCNNGG 2 cut(s) 9, 376
BssMI GATC 4 cut(s) 126, 175, 751, 768
BssSI CACGAG 1 cut(s) 18
BssT1I CCWWGG 1 cut(s) 376
Bst2BI CACGAG 1 cut(s) 18
Bst2UI CCWGG 2 cut(s) 10, 865
Bst4CI ACNGT 2 cut(s) 278, 833
Bst6I CTCTTC 3 cut(s) 195, 323, 719
BstBI TTCGAA 1 cut(s) 559
BstC8I GCNNGC 1 cut(s) 295
BstDEI CTNAG 4 cut(s) 78, 368, 741, 845
BstF5I GGATG 3 cut(s) 532, 766, 825
BstFNI CGCG 1 cut(s) 290
BstH2I RGCGCY 1 cut(s) 588
BstHHI GCGC 1 cut(s) 587
BstKTI GATC 4 cut(s) 129, 178, 754, 771
BstMAI GTCTC 2 cut(s) 182, 320
BstMBI GATC 4 cut(s) 126, 175, 751, 768
BstMWI GCNNNNNNNGC 3 cut(s) 369, 661, 703
BstNI CCWGG 2 cut(s) 10, 865
BstNSI RCATGY 1 cut(s) 232
BstSCI CCNGG 2 cut(s) 8, 863
BstSFI CTRYAG 1 cut(s) 542
BstSLI GKGCMC 1 cut(s) 798
BstUI CGCG 1 cut(s) 290
BstV1I GCAGC 3 cut(s) 107, 359, 859
BstX2I RGATCY 1 cut(s) 126
BstXI CCANNNNNNTGG 2 cut(s) 293, 507
BstYI RGATCY 1 cut(s) 126
BsuRI GGCC 1 cut(s) 363
BtgZI GCGATG 1 cut(s) 594
BtsCI GGATG 3 cut(s) 532, 766, 825
BveI ACCTGC 1 cut(s) 707
Cac8I GCNNGC 1 cut(s) 295
CfoI GCGC 1 cut(s) 587
Cfr13I GGNCC 2 cut(s) 85, 388
Csp6I GTAC 1 cut(s) 157
CviAII CATG 2 cut(s) 229, 713
CviQI GTAC 1 cut(s) 157
DdeI CTNAG 4 cut(s) 78, 368, 741, 845
DpnI GATC 4 cut(s) 128, 177, 753, 770
DpnII GATC 4 cut(s) 126, 175, 751, 768
DriI GACNNNNNGTC 1 cut(s) 386
Eam1104I CTCTTC 3 cut(s) 195, 323, 719
Eam1105I GACNNNNNGTC 1 cut(s) 386
EarI CTCTTC 3 cut(s) 195, 323, 719
EciI GGCGGA 1 cut(s) 632
Ecl136II GAGCTC 1 cut(s) 842
Eco130I CCWWGG 1 cut(s) 376
Eco24I GRGCYC 1 cut(s) 844
Eco47I GGWCC 2 cut(s) 85, 388
Eco53kI GAGCTC 1 cut(s) 842
Eco88I CYCGRG 1 cut(s) 310
EcoICRI GAGCTC 1 cut(s) 842
EcoRII CCWGG 2 cut(s) 8, 863
EcoT14I CCWWGG 1 cut(s) 376
EcoT38I GRGCYC 1 cut(s) 844
ErhI CCWWGG 1 cut(s) 376
FaeI CATG 2 cut(s) 232, 716
FatI CATG 2 cut(s) 228, 712
Fnu4HI GCNGC 6 cut(s) 96, 146, 291, 373, 662, 873
FokI GGATG 3 cut(s) 519, 773, 832
FriOI GRGCYC 1 cut(s) 844
Fsp4HI GCNGC 6 cut(s) 96, 146, 291, 373, 662, 873
FspBI CTAG 2 cut(s) 516, 837
GlaI GCGC 1 cut(s) 586
GluI GCNGC 6 cut(s) 96, 146, 291, 373, 662, 873
GsuI CTGGAG 1 cut(s) 93
HaeII RGCGCY 1 cut(s) 588
HaeIII GGCC 1 cut(s) 363
HhaI GCGC 1 cut(s) 587
Hin1II CATG 2 cut(s) 232, 716
Hin6I GCGC 1 cut(s) 585
HinP1I GCGC 1 cut(s) 585
HinfI GANTC 5 cut(s) 313, 394, 568, 643, 809
Hpy188I TCNGA 2 cut(s) 369, 573
Hpy188III TCNNGA 9 cut(s) 18, 67, 88, 341, 391, 461, 474, 536, 806
Hpy99I CGWCG 1 cut(s) 817
HpyAV CCTTC 3 cut(s) 374, 633, 848
HpyCH4III ACNGT 2 cut(s) 278, 833
HpyCH4IV ACGT 2 cut(s) 492, 789
HpyCH4V TGCA 5 cut(s) 440, 544, 697, 716, 875
HpyF10VI GCNNNNNNNGC 3 cut(s) 369, 661, 703
HpyF3I CTNAG 4 cut(s) 78, 368, 741, 845
HpySE526I ACGT 2 cut(s) 492, 789
Hsp92II CATG 2 cut(s) 232, 716
HspAI GCGC 1 cut(s) 585
Kzo9I GATC 4 cut(s) 126, 175, 751, 768
LguI GCTCTTC 1 cut(s) 719
LmnI GCTCC 1 cut(s) 74
Lsp1109I GCAGC 3 cut(s) 107, 359, 859
MaeI CTAG 2 cut(s) 516, 837
MaeII ACGT 2 cut(s) 492, 789
MaeIII GTNAC 2 cut(s) 329, 699
MalI GATC 4 cut(s) 128, 177, 753, 770
MbiI CCGCTC 1 cut(s) 729
MboI GATC 4 cut(s) 126, 175, 751, 768
MboII GAAGA 6 cut(s) 212, 310, 348, 495, 498, 736
MfeI CAATTG 1 cut(s) 425
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 3 cut(s) 603, 798, 844
MluCI AATT 5 cut(s) 192, 414, 425, 561, 691
MlyI GAGTC 3 cut(s) 322, 403, 577
MseI TTAA 5 cut(s) 195, 306, 413, 590, 666
MspA1I CMGCKG 1 cut(s) 661
MspR9I CCNGG 2 cut(s) 10, 865
MunI CAATTG 1 cut(s) 425
Mva1269I GAATGC 1 cut(s) 114
MvaI CCWGG 2 cut(s) 10, 865
MvnI CGCG 1 cut(s) 290
MwoI GCNNNNNNNGC 3 cut(s) 369, 661, 703
NdeII GATC 4 cut(s) 126, 175, 751, 768
NlaIII CATG 2 cut(s) 232, 716
NmuCI GTSAC 1 cut(s) 329
NspI RCATGY 1 cut(s) 232
NspV TTCGAA 1 cut(s) 559
PciSI GCTCTTC 1 cut(s) 719
PctI GAATGC 1 cut(s) 114
PfeI GAWTC 2 cut(s) 643, 809
PkrI GCNGC 6 cut(s) 97, 147, 292, 374, 663, 874
PleI GAGTC 3 cut(s) 321, 402, 576
PpsI GAGTC 3 cut(s) 321, 402, 576
PsiI TTATAA 1 cut(s) 690
Psp124BI GAGCTC 1 cut(s) 844
Psp1406I AACGTT 1 cut(s) 492
Psp6I CCWGG 2 cut(s) 8, 863
PspGI CCWGG 2 cut(s) 8, 863
PspPI GGNCC 2 cut(s) 85, 388
PstI CTGCAG 1 cut(s) 546
PsuI RGATCY 1 cut(s) 126
RsaI GTAC 1 cut(s) 158
RsaNI GTAC 1 cut(s) 157
SacI GAGCTC 1 cut(s) 844
SapI GCTCTTC 1 cut(s) 719
SaqAI TTAA 5 cut(s) 195, 306, 413, 590, 666
SatI GCNGC 6 cut(s) 96, 146, 291, 373, 662, 873
Sau3AI GATC 4 cut(s) 126, 175, 751, 768
Sau96I GGNCC 2 cut(s) 85, 388
SchI GAGTC 3 cut(s) 322, 403, 577
ScrFI CCNGG 2 cut(s) 10, 865
SduI GDGCHC 3 cut(s) 603, 798, 844
SfcI CTRYAG 1 cut(s) 542
SfuI TTCGAA 1 cut(s) 559
SinI GGWCC 2 cut(s) 85, 388
Sse9I AATT 5 cut(s) 192, 414, 425, 561, 691
SsiI CCGC 7 cut(s) 41, 146, 290, 554, 617, 661, 729
SspMI CTAG 2 cut(s) 516, 837
SstI GAGCTC 1 cut(s) 844
StyD4I CCNGG 2 cut(s) 8, 863
StyI CCWWGG 1 cut(s) 376
TaaI ACNGT 2 cut(s) 278, 833
TaiI ACGT 2 cut(s) 495, 792
TaqI TCGA 3 cut(s) 348, 481, 559
TasI AATT 5 cut(s) 192, 414, 425, 561, 691
TauI GCSGC 3 cut(s) 148, 293, 664
TfiI GAWTC 2 cut(s) 643, 809
Tru1I TTAA 5 cut(s) 195, 306, 413, 590, 666
Tru9I TTAA 5 cut(s) 195, 306, 413, 590, 666
TseFI GTSAC 1 cut(s) 329
TseI GCWGC 3 cut(s) 95, 372, 872
Tsp45I GTSAC 1 cut(s) 329
TspDTI ATGAA 6 cut(s) 180, 674, 701, 737, 792, 836
TspGWI ACGGA 2 cut(s) 410, 622
VpaK11BI GGWCC 2 cut(s) 85, 388
XapI RAATTY 1 cut(s) 561
XceI RCATGY 1 cut(s) 232
XcmI CCANNNNNNNNNTGG 3 cut(s) 504, 507, 762
XspI CTAG 2 cut(s) 516, 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.