RLG00000020348

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009746
Physical Location & Seq
Reverse (-)
63948949 .. 63951155
2207 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020348

Sequence Viewer

Length: 1572 bp
ATGAACACATTGGGCATTTTCACTAACATTTCTCGAATCACTAATATTCATGATCATGGAAGAGTTGTTGATAGTAAAAAGGGTAGATTTCAATGCAACTACTGTGATAAAGAGGTGAGCGGTATCACCCGTTTGAAGTGCCATTTAGGAGGAATAGGGCCGGAAGTAAAACCATGTTTAGAGGTTCCTGCTGAGGTGAAGGAGCTGTGGAGAAACAAGTTCCTTGAAAGTCGTGGCAAGAAAGTTAACCACTCTTCTAGCTCAAATGGTGTTAAGCGTAAGAAGCGTGAGACTTCTCAAACTTCTAGTTATCAGAATGGAAGCGAAGAAGATACAGACTCTATGTCAGAAGAAAATGCAACAGATTATGTCAGCAGTCCCAATGGAAATATAGGCCTTCAAAGAGGAGAAAGTAATGACATGAGAAATGTTTCAATATCAATGAACCCCCAAAGATGCATTGGTAGATTCTTTTATGAAACAGGCTTAGAATTTACTGCTGCAAATTCTCCTAGCTTCAGAATAATGATAAATTCTGTTGCTGGTCCTGGTGTGAATTACAAGATTCCTAGCTGTGAAGAGCTTAAAGGTTGCATCTTTCAAGATGAAGTGAAGGAGATGCAAGAATATGTGAGAAAGATTAAGGAATCCTGGGAACTAAAAGACATGTTTGCCTCATCTGAGTGGAACATGTCAATCTGGGGTTCGAGAGTGGAGAGCAAGATGGTAGCAGATATAGTGGAGGATCAGTCCTTTTGGACTGGAGCTGAGATGGTCTCGAAAGCAACTATGCCGCTGGTTCGTGCTATTGAATTGATCTTTGAGGCTGACAAGCCACTTGTGGGGCACATATATGAAACCATGGATCAAGTGAAGGAGAGAATTAAAGAAGAATTAATAAACAAAAAGTCCCAATACATGCCTTTTTGGGAAGTTATTGATGACATTTGGGACAATATTCTCCATATCCCTCTCCACGGGGCTGGTTATTATCTTAATCCAAGTCTCAAATATTCGAATGACTTCTATTGTGATGATGAGGTTTACCTTGGGCTTATGTGCTGCATTGCACAGATGGTGTGGGATCCTTCAATTCAAGATGTAATACACAATCAACTCAAGGAGTACATACGTGTTGAAGGCAGTTTCAAGGAGGGGAGTTCCTCTAGCCGAAGAATAACTCATCCAGCTATATGGTGGTCTTCTTATGGGAAACAATGTCCTGAACTCCAGAGGTTTGCCATTCGAGTTTTGAGCCAGAACTGCGATGGTGCTTCAAGGTATGGACTCAGAAGGACTCTAGCTGAGAAGCTGGTTACAAATGGAAGGAATCCTATCGAGCAGCAACGGTTGAGTGATCTGACTTTTCTTCATTATAATTTGCAATTACAGAAGTTTTGCTCAGGTGTGAAGAGCGGCATTGAAGCTGAGGATATTCATCCAATGGATGACTGGATCATCGATGAAGCACAAGATACTGTTTCTCAGAATGGGGCTGTGAAGGTGGAGCCTGCTAATGGAGCTACCAGTGAAGGATGTTCAAGATTTCAGCTTAAAAGAGAATCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

524

Amino Acids

59.6

Weight (kDa)

5.71

Isoelectric Point (pI)

48.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009746)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13010 AT3G13020 AT3G13030 AT3G13030 AT3G13030 AT3G13030 AT3G13030
fragaria_vesca FvH4_6g36422
malus_domestica MD09G1169400.v1.1
pyrus_communis pycom09g08680
rosa_chinensis RchiOBHm_Chr2g0148401
rosa_laevigata RLG00000020348
rosa_roxburghii Rroxscaffold_2G00098760
rosa_rugosa Rorug02G0406800
rosa_samantha Rh2AG465500 Rh2BG478400 Rh2BG478500 Rh2CG452000 Rh2DG487200 Rh2DG487300
rosa_wichuraiana Rw2G037940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1377
AccBSI CCGCTC 2 cut(s) 120, 1416
AciI CCGC 3 cut(s) 120, 794, 1416
AclWI GGATC 5 cut(s) 753, 873, 1079, 1092, 1463
AcsI RAATTY 3 cut(s) 491, 505, 532
AcuI CTGAAG 1 cut(s) 502
AfaI GTAC 1 cut(s) 1127
AfiI CCNNNNNNNGG 3 cut(s) 842, 977, 1517
AflIII ACRYGT 3 cut(s) 666, 690, 1132
AhdI GACNNNNNGTC 1 cut(s) 343
AjnI CCWGG 2 cut(s) 547, 650
Alw26I GTCTC 3 cut(s) 284, 781, 1010
AlwI GGATC 5 cut(s) 753, 873, 1079, 1092, 1463
AoxI GGCC 2 cut(s) 158, 394
ApeKI GCWGC 3 cut(s) 500, 1062, 1342
ApoI RAATTY 3 cut(s) 491, 505, 532
AseI ATTAAT 1 cut(s) 896
Asp700I GAANNNNTTC 2 cut(s) 430, 1022
AspS9I GGNCC 2 cut(s) 158, 545
AsuHPI GGTGA 3 cut(s) 118, 127, 208
AsuII TTCGAA 1 cut(s) 1016
AvaII GGWCC 1 cut(s) 545
BaeGI GKGCMC 1 cut(s) 849
BamHI GGATCC 1 cut(s) 1084
BbsI GAAGAC 1 cut(s) 1194
BbvCI CCTCAGC 2 cut(s) 192, 1428
BbvI GCAGC 3 cut(s) 487, 1049, 1354
BccI CCATC 4 cut(s) 718, 766, 1069, 1262
BciT130I CCWGG 2 cut(s) 549, 652
BclI TGATCA 1 cut(s) 52
BcoDI GTCTC 3 cut(s) 284, 781, 1010
BfaI CTAG 6 cut(s) 258, 306, 513, 570, 1167, 1301
BisI GCNGC 5 cut(s) 501, 794, 1063, 1343, 1417
BlsI GCNGC 5 cut(s) 502, 795, 1064, 1344, 1418
Bme1390I CCNGG 2 cut(s) 549, 652
Bme18I GGWCC 1 cut(s) 545
BmeRI GACNNNNNGTC 1 cut(s) 343
BmgT120I GGNCC 2 cut(s) 158, 545
BmiI GGNNCC 3 cut(s) 186, 1086, 1509
BmrFI CCNGG 2 cut(s) 549, 652
BmsI GCATC 3 cut(s) 446, 603, 609
BpiI GAAGAC 1 cut(s) 1194
BplI GAGNNNNNCTC 2 cut(s) 761, 793
BpmI CTGGAG 2 cut(s) 783, 1214
Bpu10I CCTNAGC 3 cut(s) 192, 1402, 1428
Bpu14I TTCGAA 1 cut(s) 1016
BpuEI CTTGAG 1 cut(s) 1103
Bsa29I ATCGAT 1 cut(s) 1461
BsaAI YACGTR 1 cut(s) 1133
BsaBI GATNNNNATC 1 cut(s) 1437
BsaI GGTCTC 1 cut(s) 781
BsaJI CCNNGG 4 cut(s) 651, 861, 976, 1048
BsaXI ACNNNNNCTCC 2 cut(s) 734, 764
Bsc4I CCNNNNNNNGG 3 cut(s) 842, 977, 1517
Bse1I ACTGG 3 cut(s) 766, 1457, 1527
Bse3DI GCAATG 1 cut(s) 1065
Bse8I GATNNNNATC 1 cut(s) 1437
BseBI CCWGG 2 cut(s) 549, 652
BseCI ATCGAT 1 cut(s) 1461
BseDI CCNNGG 4 cut(s) 651, 861, 976, 1048
BseGI GGATG 4 cut(s) 1183, 1438, 1453, 1541
BseJI GATNNNNATC 1 cut(s) 1437
BseLI CCNNNNNNNGG 3 cut(s) 842, 977, 1517
BseMI GCAATG 1 cut(s) 1065
BseMII CTCAG 8 cut(s) 183, 672, 759, 1296, 1303, 1416, 1419, 1499
BseNI ACTGG 3 cut(s) 766, 1457, 1527
BseRI GAGGAG 1 cut(s) 420
BseSI GKGCMC 1 cut(s) 849
BseXI GCAGC 3 cut(s) 487, 1049, 1354
BshFI GGCC 2 cut(s) 160, 396
BshVI ATCGAT 1 cut(s) 1461
BsiSI CCGG 1 cut(s) 161
BslFI GGGAC 3 cut(s) 363, 895, 965
BslI CCNNNNNNNGG 3 cut(s) 842, 977, 1517
BsmAI GTCTC 3 cut(s) 284, 781, 1010
BsmFI GGGAC 3 cut(s) 363, 895, 965
BsnI GGCC 2 cut(s) 160, 396
Bso31I GGTCTC 1 cut(s) 781
Bsp119I TTCGAA 1 cut(s) 1016
Bsp1286I GDGCHC 1 cut(s) 849
Bsp143I GATC 7 cut(s) 52, 745, 816, 865, 1084, 1357, 1455
Bsp19I CCATGG 1 cut(s) 861
BspACI CCGC 3 cut(s) 120, 794, 1416
BspANI GGCC 2 cut(s) 160, 396
BspCNI CTCAG 8 cut(s) 184, 673, 760, 1297, 1302, 1415, 1420, 1498
BspDI ATCGAT 1 cut(s) 1461
BspHI TCATGA 1 cut(s) 49
BspLI GGNNCC 3 cut(s) 186, 1086, 1509
BspPI GGATC 5 cut(s) 753, 873, 1079, 1092, 1463
BspQI GCTCTTC 2 cut(s) 573, 1406
BspT104I TTCGAA 1 cut(s) 1016
BspTNI GGTCTC 1 cut(s) 781
BsrBI CCGCTC 2 cut(s) 120, 1416
BsrDI GCAATG 1 cut(s) 1065
BsrI ACTGG 3 cut(s) 766, 1457, 1527
BssECI CCNNGG 4 cut(s) 651, 861, 976, 1048
BssMI GATC 7 cut(s) 52, 745, 816, 865, 1084, 1357, 1455
BssT1I CCWWGG 2 cut(s) 861, 1048
Bst2UI CCWGG 2 cut(s) 549, 652
Bst4CI ACNGT 3 cut(s) 104, 1350, 1480
Bst6I CTCTTC 4 cut(s) 55, 259, 573, 1406
BstBAI YACGTR 1 cut(s) 1133
BstBI TTCGAA 1 cut(s) 1016
BstC8I GCNNGC 1 cut(s) 1512
BstDEI CTNAG 9 cut(s) 192, 487, 681, 768, 1289, 1305, 1402, 1428, 1485
BstDSI CCRYGG 2 cut(s) 861, 976
BstF5I GGATG 4 cut(s) 1183, 1438, 1453, 1541
BstKTI GATC 7 cut(s) 55, 748, 819, 868, 1087, 1360, 1458
BstMAI GTCTC 3 cut(s) 284, 781, 1010
BstMBI GATC 7 cut(s) 52, 745, 816, 865, 1084, 1357, 1455
BstMWI GCNNNNNNNGC 3 cut(s) 283, 1263, 1520
BstNI CCWGG 2 cut(s) 549, 652
BstNSI RCATGY 3 cut(s) 670, 694, 922
BstSCI CCNGG 2 cut(s) 547, 650
BstSLI GKGCMC 1 cut(s) 849
BstV1I GCAGC 3 cut(s) 487, 1049, 1354
BstV2I GAAGAC 1 cut(s) 1194
BstX2I RGATCY 1 cut(s) 1084
BstXI CCANNNNNNTGG 2 cut(s) 983, 1194
BstYI RGATCY 1 cut(s) 1084
Bsu15I ATCGAT 1 cut(s) 1461
BsuRI GGCC 2 cut(s) 160, 396
BsuTUI ATCGAT 1 cut(s) 1461
BtgI CCRYGG 2 cut(s) 861, 976
BtgZI GCGATG 1 cut(s) 1281
BtsCI GGATG 4 cut(s) 1183, 1438, 1453, 1541
BtsIMutI CAGTG 1 cut(s) 1534
Cac8I GCNNGC 1 cut(s) 1512
CciI TCATGA 1 cut(s) 49
Cfr13I GGNCC 2 cut(s) 158, 545
ClaI ATCGAT 1 cut(s) 1461
Csp6I GTAC 1 cut(s) 1126
CviAII CATG 9 cut(s) 50, 56, 174, 421, 667, 691, 862, 919, 1567
CviQI GTAC 1 cut(s) 1126
DdeI CTNAG 9 cut(s) 192, 487, 681, 768, 1289, 1305, 1402, 1428, 1485
DpnI GATC 7 cut(s) 54, 747, 818, 867, 1086, 1359, 1457
DpnII GATC 7 cut(s) 52, 745, 816, 865, 1084, 1357, 1455
DriI GACNNNNNGTC 1 cut(s) 343
Eam1104I CTCTTC 4 cut(s) 55, 259, 573, 1406
Eam1105I GACNNNNNGTC 1 cut(s) 343
EarI CTCTTC 4 cut(s) 55, 259, 573, 1406
Eco130I CCWWGG 2 cut(s) 861, 1048
Eco147I AGGCCT 1 cut(s) 396
Eco31I GGTCTC 1 cut(s) 781
Eco47I GGWCC 1 cut(s) 545
Eco57I CTGAAG 1 cut(s) 502
EcoRII CCWGG 2 cut(s) 547, 650
EcoT14I CCWWGG 2 cut(s) 861, 1048
EcoT22I ATGCAT 1 cut(s) 461
ErhI CCWWGG 2 cut(s) 861, 1048
FaeI CATG 9 cut(s) 53, 59, 177, 424, 670, 694, 865, 922, 1570
FaqI GGGAC 3 cut(s) 363, 895, 965
FatI CATG 9 cut(s) 49, 55, 173, 420, 666, 690, 861, 918, 1566
FbaI TGATCA 1 cut(s) 52
Fnu4HI GCNGC 5 cut(s) 501, 794, 1063, 1343, 1417
FokI GGATG 4 cut(s) 1170, 1425, 1460, 1548
Fsp4HI GCNGC 5 cut(s) 501, 794, 1063, 1343, 1417
FspBI CTAG 6 cut(s) 258, 306, 513, 570, 1167, 1301
GluI GCNGC 5 cut(s) 501, 794, 1063, 1343, 1417
GsuI CTGGAG 2 cut(s) 783, 1214
HaeIII GGCC 2 cut(s) 160, 396
HapII CCGG 1 cut(s) 161
Hin1II CATG 9 cut(s) 53, 59, 177, 424, 670, 694, 865, 922, 1570
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 9 cut(s) 36, 338, 468, 565, 647, 1287, 1297, 1330, 1562
HpaI GTTAAC 1 cut(s) 247
HpaII CCGG 1 cut(s) 161
HphI GGTGA 3 cut(s) 118, 127, 208
Hpy166II GTNNAC 2 cut(s) 247, 1045
Hpy188I TCNGA 7 cut(s) 315, 349, 521, 682, 1292, 1362, 1488
Hpy188III TCNNGA 9 cut(s) 33, 50, 602, 708, 778, 1097, 1223, 1231, 1542
Hpy8I GTNNAC 2 cut(s) 247, 1045
HpyCH4III ACNGT 3 cut(s) 104, 1350, 1480
HpyCH4IV ACGT 1 cut(s) 1132
HpyCH4V TGCA 9 cut(s) 96, 359, 459, 503, 594, 622, 1065, 1070, 1384
HpyF10VI GCNNNNNNNGC 3 cut(s) 283, 1263, 1520
HpyF3I CTNAG 9 cut(s) 192, 487, 681, 768, 1289, 1305, 1402, 1428, 1485
HpySE526I ACGT 1 cut(s) 1132
Hsp92II CATG 9 cut(s) 53, 59, 177, 424, 670, 694, 865, 922, 1570
Ksp22I TGATCA 1 cut(s) 52
KspAI GTTAAC 1 cut(s) 247
Kzo9I GATC 7 cut(s) 52, 745, 816, 865, 1084, 1357, 1455
LguI GCTCTTC 2 cut(s) 573, 1406
LmnI GCTCC 4 cut(s) 202, 764, 1507, 1520
Lsp1109I GCAGC 3 cut(s) 487, 1049, 1354
LweI GCATC 3 cut(s) 446, 603, 609
MaeI CTAG 6 cut(s) 258, 306, 513, 570, 1167, 1301
MaeII ACGT 1 cut(s) 1132
MaeIII GTNAC 1 cut(s) 1315
MalI GATC 7 cut(s) 54, 747, 818, 867, 1086, 1359, 1457
MbiI CCGCTC 2 cut(s) 120, 1416
MboI GATC 7 cut(s) 52, 745, 816, 865, 1084, 1357, 1455
MflI RGATCY 1 cut(s) 1084
MhlI GDGCHC 1 cut(s) 849
MlyI GAGTC 3 cut(s) 332, 1281, 1291
Mph1103I ATGCAT 1 cut(s) 461
MroXI GAANNNNTTC 2 cut(s) 430, 1022
MseI TTAA 8 cut(s) 246, 273, 585, 642, 885, 896, 996, 1554
MslI CAYNNNNRTG 3 cut(s) 54, 682, 852
MspA1I CMGCKG 1 cut(s) 796
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 2 cut(s) 549, 652
MvaI CCWGG 2 cut(s) 549, 652
MwoI GCNNNNNNNGC 3 cut(s) 283, 1263, 1520
NcoI CCATGG 1 cut(s) 861
NdeII GATC 7 cut(s) 52, 745, 816, 865, 1084, 1357, 1455
NlaIII CATG 9 cut(s) 53, 59, 177, 424, 670, 694, 865, 922, 1570
NlaIV GGNNCC 3 cut(s) 186, 1086, 1509
NsiI ATGCAT 1 cut(s) 461
NspI RCATGY 3 cut(s) 670, 694, 922
NspV TTCGAA 1 cut(s) 1016
PagI TCATGA 1 cut(s) 49
PceI AGGCCT 1 cut(s) 396
PciI ACATGT 2 cut(s) 666, 690
PciSI GCTCTTC 2 cut(s) 573, 1406
PdmI GAANNNNTTC 2 cut(s) 430, 1022
PfeI GAWTC 6 cut(s) 36, 468, 565, 647, 1330, 1562
PkrI GCNGC 5 cut(s) 502, 795, 1064, 1344, 1418
PleI GAGTC 3 cut(s) 332, 1281, 1291
PpsI GAGTC 3 cut(s) 332, 1281, 1291
Ppu21I YACGTR 1 cut(s) 1133
PscI ACATGT 2 cut(s) 666, 690
PshBI ATTAAT 1 cut(s) 896
PsiI TTATAA 1 cut(s) 1377
Psp6I CCWGG 2 cut(s) 547, 650
PspGI CCWGG 2 cut(s) 547, 650
PspN4I GGNNCC 3 cut(s) 186, 1086, 1509
PspPI GGNCC 2 cut(s) 158, 545
PsuI RGATCY 1 cut(s) 1084
RsaI GTAC 1 cut(s) 1127
RsaNI GTAC 1 cut(s) 1126
RseI CAYNNNNRTG 3 cut(s) 54, 682, 852
SapI GCTCTTC 2 cut(s) 573, 1406
SaqAI TTAA 8 cut(s) 246, 273, 585, 642, 885, 896, 996, 1554
SatI GCNGC 5 cut(s) 501, 794, 1063, 1343, 1417
Sau3AI GATC 7 cut(s) 52, 745, 816, 865, 1084, 1357, 1455
Sau96I GGNCC 2 cut(s) 158, 545
SchI GAGTC 3 cut(s) 332, 1281, 1291
ScrFI CCNGG 2 cut(s) 549, 652
SduI GDGCHC 1 cut(s) 849
SfaNI GCATC 3 cut(s) 446, 603, 609
SfuI TTCGAA 1 cut(s) 1016
SinI GGWCC 1 cut(s) 545
SmiMI CAYNNNNRTG 3 cut(s) 54, 682, 852
SmlI CTYRAG 1 cut(s) 1118
SmoI CTYRAG 1 cut(s) 1118
SseBI AGGCCT 1 cut(s) 396
SsiI CCGC 3 cut(s) 120, 794, 1416
SspI AATATT 3 cut(s) 46, 958, 1013
SspMI CTAG 6 cut(s) 258, 306, 513, 570, 1167, 1301
StuI AGGCCT 1 cut(s) 396
StyD4I CCNGG 2 cut(s) 547, 650
StyI CCWWGG 2 cut(s) 861, 1048
TaaI ACNGT 3 cut(s) 104, 1350, 1480
TaiI ACGT 1 cut(s) 1135
TaqI TCGA 7 cut(s) 34, 707, 779, 1016, 1246, 1338, 1461
TatI WGTACW 1 cut(s) 1125
TauI GCSGC 2 cut(s) 796, 1419
TfiI GAWTC 6 cut(s) 36, 468, 565, 647, 1330, 1562
Tru1I TTAA 8 cut(s) 246, 273, 585, 642, 885, 896, 996, 1554
Tru9I TTAA 8 cut(s) 246, 273, 585, 642, 885, 896, 996, 1554
TscAI CASTG 1 cut(s) 1534
TseI GCWGC 3 cut(s) 500, 1062, 1342
TspDTI ATGAA 9 cut(s) 17, 38, 458, 492, 621, 870, 1361, 1427, 1479
TspRI CASTG 1 cut(s) 1534
VpaK11BI GGWCC 1 cut(s) 545
VspI ATTAAT 1 cut(s) 896
XapI RAATTY 3 cut(s) 491, 505, 532
XceI RCATGY 3 cut(s) 670, 694, 922
XcmI CCANNNNNNNNNTGG 4 cut(s) 458, 1194, 1265, 1449
XmnI GAANNNNTTC 2 cut(s) 430, 1022
XspI CTAG 6 cut(s) 258, 306, 513, 570, 1167, 1301
Zsp2I ATGCAT 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.