Rroxscaffold_2G00098760

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009746
Physical Location & Seq
Forward (+)
20222151 .. 20224527
2377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00098760.1

Sequence Viewer

Length: 1281 bp
ATGAACACATTGGGCATTTTCACTAACATTTCTCGAATCACTAATATTCATGATCATGGAAGAGTTGTTGATAGTAAAAAGGGTAGATTTCAATGCAACTACTGTGATAAAGAGGTGAGCGGTATCACCCGTTTGAAGTGCCATTTAGGAGGAGTAGGGCCGGAAGTAAAACCATGTTTAGAGGTTCCTGTTGAGGTGAAGGAGCTGTGGAGAAACAAGTTGCTAGAAAGTCGCGGCGAGAAAGTTAACCACTCTTCCAGCTCAAATGGTGTTAAGCGTAAGAGGCGTGAGACTTTTCAAACTTCTAGTTATCAAGATGAAAGTGAAGAAGATACAGACTCTATGTCAGAAGAAAATGCAACAGATCATGTCAGCAGTCCCAATGGAAATATAGGCCTTCAAAGAGGAGAAAGTAATGACATGAGAAATGTTTCGATATCAATGAACCCCCAAAGATGCATTGGTAGATTCTTTTATGAAACAGGCTTAGAATTTACTGCTGCAAATTCTCCTAGCTTCAAAAGAATGATAAATTCTGTTGCTGGTCCTGGTGTGAATTACAAGATTCCTAGCTGTGAAGAGCTTAAAGGTTGCATCTTTCAAGATGAAGTGAAGGAGATGCAAGAATATGTGAGAAAGATTAAGGAATCCTGGGCTACTACTGGATGTAGCATCTTGTTGGACAGTTGGATTGATGAAAAGGGTAGAAACCTCGTTAATTTTTTGGTCAACTGCCCTCAAGGCCCTGTTTATCTCTGTACCCATGATGTTTCATCTTCCATTGGTAATGTCGATGCCATACGTGCACTAGTGGAGGGAATTATTAAGGATGTTGGGGCTGACAATGTTGTTCAGATTGTGGCAGATTCTACAACAGGCTGGGTTGGTGCTCTAGGCAAGGAATTCAGTAAGCGCAAAGGAGTGTTTTGGGCAGTGAGTGGATCTCATTGCATTGAACTGATGCTAGAGAAGATTGGAATGAGTACCTCCACTAAGGGGATTTTGGATAAAGCGAAGACTATTACAAAGTTCATTCATGGGCATGAAGAAGTTTTGAAGATTTTGAAGAAACATACTTGTGGCCGTGGCCTGATTAAGCCCTCCAAGATAAGAGGAGCAATGCCCTTTATGACTTTAGATAGAATTGTATACGAAAAGCAGGAACTAAAAGACATGTTTGCCTCATCTGAGTGGAACATGTCAATCTGGGGTTCGAGAGTGGAGAGCAAGATGGTAGCAGATATAGTAGAGGATCAGTCTTTTTTTTTGGACTGGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

426

Amino Acids

47.67

Weight (kDa)

7.12

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 190 - 341 4.4e-48 Domain of unknown function (DUF659)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009746)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13010 AT3G13020 AT3G13030 AT3G13030 AT3G13030 AT3G13030 AT3G13030
fragaria_vesca FvH4_6g36422
malus_domestica MD09G1169400.v1.1
pyrus_communis pycom09g08680
rosa_chinensis RchiOBHm_Chr2g0148401
rosa_laevigata RLG00000020348
rosa_roxburghii Rroxscaffold_2G00098760
rosa_rugosa Rorug02G0406800
rosa_samantha Rh2AG465500 Rh2BG478400 Rh2BG478500 Rh2CG452000 Rh2DG487200 Rh2DG487300
rosa_wichuraiana Rw2G037940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 120
AccI GTMKAC 1 cut(s) 1149
AccII CGCG 1 cut(s) 234
AciI CCGC 2 cut(s) 120, 234
AclWI GGATC 2 cut(s) 949, 1260
AcoI YGGCCR 1 cut(s) 1081
AcsI RAATTY 4 cut(s) 491, 505, 532, 902
AfaI GTAC 2 cut(s) 760, 985
AfiI CCNNNNNNNGG 1 cut(s) 996
AflIII ACRYGT 2 cut(s) 1173, 1197
AgsI TTSAA 9 cut(s) 92, 136, 299, 401, 520, 602, 956, 1057, 1066
AhdI GACNNNNNGTC 1 cut(s) 343
AhlI ACTAGT 1 cut(s) 808
AjnI CCWGG 2 cut(s) 547, 650
AluBI AGCT 6 cut(s) 205, 261, 516, 573, 583, 1278
AluI AGCT 6 cut(s) 205, 261, 516, 573, 583, 1278
Alw21I GWGCWC 2 cut(s) 808, 892
Alw26I GTCTC 1 cut(s) 284
Alw44I GTGCAC 1 cut(s) 804
AlwI GGATC 2 cut(s) 949, 1260
AoxI GGCC 5 cut(s) 158, 394, 742, 1081, 1087
ApaLI GTGCAC 1 cut(s) 804
ApeKI GCWGC 1 cut(s) 500
ApoI RAATTY 4 cut(s) 491, 505, 532, 902
Asp700I GAANNNNTTC 1 cut(s) 430
AspLEI GCGC 1 cut(s) 915
AspS9I GGNCC 3 cut(s) 158, 545, 743
AsuHPI GGTGA 3 cut(s) 118, 127, 208
AvaII GGWCC 1 cut(s) 545
BaeGI GKGCMC 1 cut(s) 808
BbsI GAAGAC 1 cut(s) 1022
Bbv12I GWGCWC 2 cut(s) 808, 892
BbvI GCAGC 1 cut(s) 487
BccI CCATC 1 cut(s) 1225
BceAI ACGGC 1 cut(s) 1068
BciT130I CCWGG 2 cut(s) 549, 652
BclI TGATCA 1 cut(s) 52
BcoDI GTCTC 1 cut(s) 284
BcuI ACTAGT 1 cut(s) 808
BfaI CTAG 7 cut(s) 224, 306, 513, 570, 809, 893, 965
BglI GCCNNNNNGGC 1 cut(s) 741
BisI GCNGC 2 cut(s) 235, 501
BlsI GCNGC 2 cut(s) 236, 502
Bme1390I CCNGG 2 cut(s) 549, 652
Bme18I GGWCC 1 cut(s) 545
BmeRI GACNNNNNGTC 1 cut(s) 343
BmgT120I GGNCC 3 cut(s) 158, 545, 743
BmiI GGNNCC 1 cut(s) 186
BmrFI CCNGG 2 cut(s) 549, 652
BmsI GCATC 6 cut(s) 446, 603, 609, 681, 784, 951
BpiI GAAGAC 1 cut(s) 1022
BplI GAGNNNNNCTC 2 cut(s) 928, 960
BpuEI CTTGAG 1 cut(s) 723
BsaAI YACGTR 1 cut(s) 803
BsaJI CCNNGG 2 cut(s) 651, 1084
Bsc4I CCNNNNNNNGG 1 cut(s) 996
Bse1I ACTGG 2 cut(s) 667, 1277
Bse3DI GCAATG 2 cut(s) 946, 1125
BseBI CCWGG 2 cut(s) 549, 652
BseDI CCNNGG 2 cut(s) 651, 1084
BseGI GGATG 2 cut(s) 671, 835
BseLI CCNNNNNNNGG 1 cut(s) 996
BseMI GCAATG 2 cut(s) 946, 1125
BseMII CTCAG 1 cut(s) 1179
BseNI ACTGG 2 cut(s) 667, 1277
BseRI GAGGAG 3 cut(s) 165, 420, 1128
BseSI GKGCMC 1 cut(s) 808
BseXI GCAGC 1 cut(s) 487
BseYI CCCAGC 1 cut(s) 879
Bsh1236I CGCG 1 cut(s) 234
BshFI GGCC 5 cut(s) 160, 396, 744, 1083, 1089
BsiHKAI GWGCWC 2 cut(s) 808, 892
BsiSI CCGG 1 cut(s) 161
BslFI GGGAC 1 cut(s) 363
BslI CCNNNNNNNGG 1 cut(s) 996
BsmAI GTCTC 1 cut(s) 284
BsmFI GGGAC 1 cut(s) 363
BsnI GGCC 5 cut(s) 160, 396, 744, 1083, 1089
Bsp1286I GDGCHC 2 cut(s) 808, 892
Bsp143I GATC 4 cut(s) 52, 364, 941, 1252
BspACI CCGC 2 cut(s) 120, 234
BspANI GGCC 5 cut(s) 160, 396, 744, 1083, 1089
BspCNI CTCAG 1 cut(s) 1180
BspFNI CGCG 1 cut(s) 234
BspHI TCATGA 1 cut(s) 49
BspLI GGNNCC 1 cut(s) 186
BspPI GGATC 2 cut(s) 949, 1260
BspQI GCTCTTC 1 cut(s) 573
BsrBI CCGCTC 1 cut(s) 120
BsrDI GCAATG 2 cut(s) 946, 1125
BsrI ACTGG 2 cut(s) 667, 1277
BssECI CCNNGG 2 cut(s) 651, 1084
BssMI GATC 4 cut(s) 52, 364, 941, 1252
BssNAI GTATAC 1 cut(s) 1150
Bst1107I GTATAC 1 cut(s) 1150
Bst2UI CCWGG 2 cut(s) 549, 652
Bst4CI ACNGT 2 cut(s) 104, 686
Bst6I CTCTTC 3 cut(s) 55, 259, 573
BstBAI YACGTR 1 cut(s) 803
BstDEI CTNAG 3 cut(s) 487, 993, 1188
BstDSI CCRYGG 1 cut(s) 1084
BstF5I GGATG 2 cut(s) 671, 835
BstFNI CGCG 1 cut(s) 234
BstHHI GCGC 1 cut(s) 915
BstKTI GATC 4 cut(s) 55, 367, 944, 1255
BstMAI GTCTC 1 cut(s) 284
BstMBI GATC 4 cut(s) 52, 364, 941, 1252
BstMWI GCNNNNNNNGC 3 cut(s) 283, 741, 803
BstNI CCWGG 2 cut(s) 549, 652
BstNSI RCATGY 2 cut(s) 1177, 1201
BstSCI CCNGG 2 cut(s) 547, 650
BstSLI GKGCMC 1 cut(s) 808
BstUI CGCG 1 cut(s) 234
BstV1I GCAGC 1 cut(s) 487
BstV2I GAAGAC 1 cut(s) 1022
BstX2I RGATCY 1 cut(s) 941
BstYI RGATCY 1 cut(s) 941
BstZ17I GTATAC 1 cut(s) 1150
BsuRI GGCC 5 cut(s) 160, 396, 744, 1083, 1089
BtgI CCRYGG 1 cut(s) 1084
BtsCI GGATG 2 cut(s) 671, 835
BtsI GCAGTG 1 cut(s) 939
BtsIMutI CAGTG 1 cut(s) 939
CciI TCATGA 1 cut(s) 49
CfoI GCGC 1 cut(s) 915
Cfr13I GGNCC 3 cut(s) 158, 545, 743
Csp6I GTAC 2 cut(s) 759, 984
CviQI GTAC 2 cut(s) 759, 984
DdeI CTNAG 3 cut(s) 487, 993, 1188
DpnI GATC 4 cut(s) 54, 366, 943, 1254
DpnII GATC 4 cut(s) 52, 364, 941, 1252
DriI GACNNNNNGTC 1 cut(s) 343
EaeI YGGCCR 1 cut(s) 1081
Eam1104I CTCTTC 3 cut(s) 55, 259, 573
Eam1105I GACNNNNNGTC 1 cut(s) 343
EarI CTCTTC 3 cut(s) 55, 259, 573
Eco147I AGGCCT 1 cut(s) 396
Eco32I GATATC 1 cut(s) 438
Eco47I GGWCC 1 cut(s) 545
EcoO109I RGGNCCY 1 cut(s) 743
EcoRI GAATTC 1 cut(s) 902
EcoRII CCWGG 2 cut(s) 547, 650
EcoRV GATATC 1 cut(s) 438
EcoT22I ATGCAT 1 cut(s) 461
FaqI GGGAC 1 cut(s) 363
FbaI TGATCA 1 cut(s) 52
FblI GTMKAC 1 cut(s) 1149
Fnu4HI GCNGC 2 cut(s) 235, 501
FokI GGATG 2 cut(s) 678, 842
Fsp4HI GCNGC 2 cut(s) 235, 501
FspBI CTAG 7 cut(s) 224, 306, 513, 570, 809, 893, 965
GlaI GCGC 1 cut(s) 914
GluI GCNGC 2 cut(s) 235, 501
GsaI CCCAGC 1 cut(s) 883
HaeIII GGCC 5 cut(s) 160, 396, 744, 1083, 1089
HapII CCGG 1 cut(s) 161
HhaI GCGC 1 cut(s) 915
Hin6I GCGC 1 cut(s) 913
HinP1I GCGC 1 cut(s) 913
HincII GTYRAC 2 cut(s) 247, 730
HindII GTYRAC 2 cut(s) 247, 730
HinfI GANTC 6 cut(s) 36, 338, 468, 565, 647, 866
HpaI GTTAAC 1 cut(s) 247
HpaII CCGG 1 cut(s) 161
HphI GGTGA 3 cut(s) 118, 127, 208
Hpy166II GTNNAC 4 cut(s) 247, 730, 806, 1150
Hpy188I TCNGA 3 cut(s) 349, 855, 1189
Hpy188III TCNNGA 5 cut(s) 33, 50, 314, 602, 1215
Hpy8I GTNNAC 4 cut(s) 247, 730, 806, 1150
HpyAV CCTTC 3 cut(s) 193, 407, 607
HpyCH4III ACNGT 2 cut(s) 104, 686
HpyCH4IV ACGT 1 cut(s) 802
HpyCH4V TGCA 8 cut(s) 96, 359, 459, 503, 594, 622, 806, 951
HpyF10VI GCNNNNNNNGC 3 cut(s) 283, 741, 803
HpyF3I CTNAG 3 cut(s) 487, 993, 1188
HpySE526I ACGT 1 cut(s) 802
HspAI GCGC 1 cut(s) 913
Ksp22I TGATCA 1 cut(s) 52
KspAI GTTAAC 1 cut(s) 247
Kzo9I GATC 4 cut(s) 52, 364, 941, 1252
LguI GCTCTTC 1 cut(s) 573
LmnI GCTCC 3 cut(s) 202, 1115, 1275
Lsp1109I GCAGC 1 cut(s) 487
LweI GCATC 6 cut(s) 446, 603, 609, 681, 784, 951
MaeI CTAG 7 cut(s) 224, 306, 513, 570, 809, 893, 965
MaeII ACGT 1 cut(s) 802
MalI GATC 4 cut(s) 54, 366, 943, 1254
MbiI CCGCTC 1 cut(s) 120
MboI GATC 4 cut(s) 52, 364, 941, 1252
MflI RGATCY 1 cut(s) 941
MhlI GDGCHC 2 cut(s) 808, 892
MluCI AATT 8 cut(s) 491, 505, 532, 556, 718, 819, 902, 1143
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 2 cut(s) 660, 668
Mph1103I ATGCAT 1 cut(s) 461
MroXI GAANNNNTTC 1 cut(s) 430
MseI TTAA 7 cut(s) 246, 273, 585, 642, 717, 825, 1095
MslI CAYNNNNRTG 4 cut(s) 54, 1041, 1077, 1189
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 2 cut(s) 549, 652
MvaI CCWGG 2 cut(s) 549, 652
MvnI CGCG 1 cut(s) 234
MwoI GCNNNNNNNGC 3 cut(s) 283, 741, 803
NdeII GATC 4 cut(s) 52, 364, 941, 1252
NlaIV GGNNCC 1 cut(s) 186
NsiI ATGCAT 1 cut(s) 461
NspI RCATGY 2 cut(s) 1177, 1201
PagI TCATGA 1 cut(s) 49
PceI AGGCCT 1 cut(s) 396
PciI ACATGT 2 cut(s) 1173, 1197
PciSI GCTCTTC 1 cut(s) 573
PdmI GAANNNNTTC 1 cut(s) 430
PfeI GAWTC 5 cut(s) 36, 468, 565, 647, 866
PkrI GCNGC 2 cut(s) 236, 502
PleI GAGTC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 332
Ppu21I YACGTR 1 cut(s) 803
PscI ACATGT 2 cut(s) 1173, 1197
Psp6I CCWGG 2 cut(s) 547, 650
PspFI CCCAGC 1 cut(s) 879
PspGI CCWGG 2 cut(s) 547, 650
PspN4I GGNNCC 1 cut(s) 186
PspPI GGNCC 3 cut(s) 158, 545, 743
PsuI RGATCY 1 cut(s) 941
RsaI GTAC 2 cut(s) 760, 985
RsaNI GTAC 2 cut(s) 759, 984
RseI CAYNNNNRTG 4 cut(s) 54, 1041, 1077, 1189
SapI GCTCTTC 1 cut(s) 573
SaqAI TTAA 7 cut(s) 246, 273, 585, 642, 717, 825, 1095
SatI GCNGC 2 cut(s) 235, 501
Sau3AI GATC 4 cut(s) 52, 364, 941, 1252
Sau96I GGNCC 3 cut(s) 158, 545, 743
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 2 cut(s) 549, 652
SduI GDGCHC 2 cut(s) 808, 892
SfaNI GCATC 6 cut(s) 446, 603, 609, 681, 784, 951
SinI GGWCC 1 cut(s) 545
SmiMI CAYNNNNRTG 4 cut(s) 54, 1041, 1077, 1189
SmlI CTYRAG 1 cut(s) 738
SmoI CTYRAG 1 cut(s) 738
SpeI ACTAGT 1 cut(s) 808
Sse9I AATT 8 cut(s) 491, 505, 532, 556, 718, 819, 902, 1143
SseBI AGGCCT 1 cut(s) 396
SsiI CCGC 2 cut(s) 120, 234
SspI AATATT 1 cut(s) 46
SspMI CTAG 7 cut(s) 224, 306, 513, 570, 809, 893, 965
StuI AGGCCT 1 cut(s) 396
StyD4I CCNGG 2 cut(s) 547, 650
TaaI ACNGT 2 cut(s) 104, 686
TaiI ACGT 1 cut(s) 805
TaqI TCGA 4 cut(s) 34, 434, 792, 1214
TasI AATT 8 cut(s) 491, 505, 532, 556, 718, 819, 902, 1143
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 5 cut(s) 36, 468, 565, 647, 866
Tru1I TTAA 7 cut(s) 246, 273, 585, 642, 717, 825, 1095
Tru9I TTAA 7 cut(s) 246, 273, 585, 642, 717, 825, 1095
TscAI CASTG 1 cut(s) 939
TseI GCWGC 1 cut(s) 500
TspRI CASTG 1 cut(s) 939
VneI GTGCAC 1 cut(s) 804
VpaK11BI GGWCC 1 cut(s) 545
XapI RAATTY 4 cut(s) 491, 505, 532, 902
XceI RCATGY 2 cut(s) 1177, 1201
XcmI CCANNNNNNNNNTGG 1 cut(s) 458
XmiI GTMKAC 1 cut(s) 1149
XmnI GAANNNNTTC 1 cut(s) 430
XspI CTAG 7 cut(s) 224, 306, 513, 570, 809, 893, 965
Zsp2I ATGCAT 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.