Rh2CG452000

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0009746
Physical Location & Seq
Reverse (-)
61334930 .. 61338457
3528 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG452000.1

Sequence Viewer

Length: 1719 bp
ATGAACACATTGGGCATTTTCACTAACATTTCTCGAATCACTAATATTCATGATCATGGAAGAGTTATTGATAGTAAAAAGGGTAGATTTCAATGCAACTACTGTGATAAAGAGGTGAGCGGTATCACCCGTTTGAAGTGCCATTTAGGAGGAATAGGGCCGGAAGTAAAACCATGTTTAGAGGTTCCTGCTGAGGTGAAGGAGCTGTGGAGAAACAAGTTGCTTGAAAGTCGTGGCAAGAAAGTTAACCACTCTTCCAGCTCAAATGGTGTTAAGCGTAAGTTACGTGAGACTTCTCAAACTTCTAGTTATCAGGATGGAAGCGAAGAAAATACAGACTCTATGTCAGAAGAAAATGCAACAGATTATGTCAACAGTCCCAATGGAAATATAGGCCTTCAAAGAGGAGAAAGTAATGACATGAGAAATGTTTCGATATCAATGAACCCCCAAAGATGCATTGGTAGATTCTTTTATGAAACAGGCTTAGAATTTACTGCTGCAAATTCTCCTAGCTTCAAAAGAATGATAAATTCTGTTGCTGGTCCTGGTGTGAATTACAAGATTCCTAGCTGTGAAGAGCTTAAAGGTTGCATCTTTCAAGATGAAGTGAAGGAGATGCAAGAATATGTGAGAAAGATTAAGGAATCCTGGGCTACTACTGGATGTAGCATCTTGTTGGACAGTTGGATTGATGAAAAGGGTAGAAACCTCGTTAACTTTTTGGTCAACTGCCCTCAAGGCCCTGTTTATCTCTGTACTCATGATGTTTCATCTTCCATTGGTAATGTCGATGCCATACGTGCACTATTGGAGGGGATTATTAAGGATGTTGGGGCTGACAATGTTGTTCAGATTGTGGCAGATTCTACAACAGGCTGGGTTGGTGCTCTAGGCAAGGAGTTCAGTAAGCACAAAGGAGTGTTTTGGGCAGTGAGTGGATCTCATTGCATTGAGCTGATGCTAGAGAAGATTGGAATGAGTACCTCCACTAAGGGGATTTTGGATAAAGCGAAGACTATTACAAAATTCATTCATGGGCATGAAGAAGTTTTGAAGATTTTGAAGAAACATACTTGTGGCCGTGACCTGATTAAGCCCTCCAAGATAAGAGGAGCAATGCCCTTTATGACTTTAGATAGTATTGTATACGAAAAGCAGGAACTAAAAGACATGTTTGCCTCATCTGAGTGGAACATGTCAATCTGGGGTTCGAGAGTGGAGAGCAAGATGGTAGCAGATATAGTGGAAGATCAGTCCTTTTGGACTAGAGCTGAGATGGTCTCGAAAGCAACTATGCCGCTGGTTCGTGCTATTGAATTGATCTTTGAGGCTGACAAGCCACTTGTGGGGCACATATACGAAACCATGGATCAAGTGAAGGAGAGAATTAAAGAAGAATTAATAAACAAAAAGTCCCAATACAGGCCTTTTTGGGAAGTTATCGATGACATTTGGGACAATATTCTCCATATCCCTCTCCACGGGGCTGGTTATTATCTTAATCCAAGTCTCAAATATTCTAATGACTTCTATTGCGATGATGAGGTTTACCTTGGGCTTATGTGCTGCATTGCACAGATGGTGCGGGATCCTTCAATTCAAGATGTAATACACAATCAACTCAAGGAGTACATACGTGCTGAAGGCAGTTTCAAGGAGGGGAGTTCCTCTAGCCGAAGAATAACTCATCCAGGTAAAGAAGATAAAAATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

572

Amino Acids

64.49

Weight (kDa)

6.46

Isoelectric Point (pI)

38.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 190 - 341 5.5e-48 Domain of unknown function (DUF659)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009746)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13010 AT3G13020 AT3G13030 AT3G13030 AT3G13030 AT3G13030 AT3G13030
fragaria_vesca FvH4_6g36422
malus_domestica MD09G1169400.v1.1
pyrus_communis pycom09g08680
rosa_chinensis RchiOBHm_Chr2g0148401
rosa_laevigata RLG00000020348
rosa_roxburghii Rroxscaffold_2G00098760
rosa_rugosa Rorug02G0406800
rosa_samantha Rh2AG465500 Rh2BG478400 Rh2BG478500 Rh2CG452000 Rh2DG487200 Rh2DG487300
rosa_wichuraiana Rw2G037940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 120
AccI GTMKAC 1 cut(s) 1149
AciI CCGC 3 cut(s) 120, 1301, 1588
AclWI GGATC 4 cut(s) 949, 1380, 1586, 1599
AcoI YGGCCR 1 cut(s) 1081
AcsI RAATTY 4 cut(s) 491, 505, 532, 1028
AcuI CTGAAG 1 cut(s) 1665
AfaI GTAC 3 cut(s) 760, 985, 1634
AfiI CCNNNNNNNGG 4 cut(s) 996, 1349, 1425, 1484
AflIII ACRYGT 2 cut(s) 1173, 1197
AhdI GACNNNNNGTC 1 cut(s) 343
AjnI CCWGG 3 cut(s) 547, 650, 1693
AluBI AGCT 7 cut(s) 205, 261, 516, 573, 583, 958, 1274
AluI AGCT 7 cut(s) 205, 261, 516, 573, 583, 958, 1274
Alw21I GWGCWC 2 cut(s) 808, 892
Alw26I GTCTC 3 cut(s) 284, 1288, 1517
Alw44I GTGCAC 1 cut(s) 804
AlwI GGATC 4 cut(s) 949, 1380, 1586, 1599
AoxI GGCC 5 cut(s) 158, 394, 742, 1081, 1427
ApaLI GTGCAC 1 cut(s) 804
ApeKI GCWGC 2 cut(s) 500, 1569
ApoI RAATTY 4 cut(s) 491, 505, 532, 1028
AseI ATTAAT 1 cut(s) 1403
Asp700I GAANNNNTTC 1 cut(s) 430
AspS9I GGNCC 3 cut(s) 158, 545, 743
AsuHPI GGTGA 3 cut(s) 118, 127, 208
AvaII GGWCC 1 cut(s) 545
BaeGI GKGCMC 2 cut(s) 808, 1356
BamHI GGATCC 1 cut(s) 1591
BbsI GAAGAC 1 cut(s) 1022
Bbv12I GWGCWC 2 cut(s) 808, 892
BbvCI CCTCAGC 1 cut(s) 192
BbvI GCAGC 2 cut(s) 487, 1556
BccI CCATC 4 cut(s) 311, 1225, 1273, 1576
BceAI ACGGC 1 cut(s) 1068
BciT130I CCWGG 3 cut(s) 549, 652, 1695
BclI TGATCA 1 cut(s) 52
BcoDI GTCTC 3 cut(s) 284, 1288, 1517
BfaI CTAG 7 cut(s) 306, 513, 570, 893, 965, 1269, 1674
BglI GCCNNNNNGGC 1 cut(s) 741
BisI GCNGC 3 cut(s) 501, 1301, 1570
BlsI GCNGC 3 cut(s) 502, 1302, 1571
Bme1390I CCNGG 3 cut(s) 549, 652, 1695
Bme18I GGWCC 1 cut(s) 545
BmeRI GACNNNNNGTC 1 cut(s) 343
BmgT120I GGNCC 3 cut(s) 158, 545, 743
BmiI GGNNCC 2 cut(s) 186, 1593
BmrFI CCNGG 3 cut(s) 549, 652, 1695
BmsI GCATC 6 cut(s) 446, 603, 609, 681, 784, 951
BpiI GAAGAC 1 cut(s) 1022
BplI GAGNNNNNCTC 4 cut(s) 928, 960, 1268, 1300
Bpu10I CCTNAGC 1 cut(s) 192
BpuEI CTTGAG 2 cut(s) 723, 1610
Bsa29I ATCGAT 1 cut(s) 1446
BsaAI YACGTR 3 cut(s) 287, 803, 1640
BsaI GGTCTC 1 cut(s) 1288
BsaJI CCNNGG 4 cut(s) 651, 1368, 1483, 1555
Bsc4I CCNNNNNNNGG 4 cut(s) 996, 1349, 1425, 1484
Bse1I ACTGG 1 cut(s) 667
Bse3DI GCAATG 3 cut(s) 946, 1125, 1572
BseBI CCWGG 3 cut(s) 549, 652, 1695
BseCI ATCGAT 1 cut(s) 1446
BseDI CCNNGG 4 cut(s) 651, 1368, 1483, 1555
BseGI GGATG 4 cut(s) 322, 671, 835, 1690
BseLI CCNNNNNNNGG 4 cut(s) 996, 1349, 1425, 1484
BseMI GCAATG 3 cut(s) 946, 1125, 1572
BseMII CTCAG 3 cut(s) 183, 1179, 1266
BseNI ACTGG 1 cut(s) 667
BseRI GAGGAG 2 cut(s) 420, 1128
BseSI GKGCMC 2 cut(s) 808, 1356
BseXI GCAGC 2 cut(s) 487, 1556
BseYI CCCAGC 1 cut(s) 879
BshFI GGCC 5 cut(s) 160, 396, 744, 1083, 1429
BshVI ATCGAT 1 cut(s) 1446
BsiHKAI GWGCWC 2 cut(s) 808, 892
BsiSI CCGG 1 cut(s) 161
BslFI GGGAC 3 cut(s) 363, 1402, 1472
BslI CCNNNNNNNGG 4 cut(s) 996, 1349, 1425, 1484
BsmAI GTCTC 3 cut(s) 284, 1288, 1517
BsmFI GGGAC 3 cut(s) 363, 1402, 1472
BsnI GGCC 5 cut(s) 160, 396, 744, 1083, 1429
Bso31I GGTCTC 1 cut(s) 1288
Bsp1286I GDGCHC 3 cut(s) 808, 892, 1356
Bsp143I GATC 6 cut(s) 52, 941, 1252, 1323, 1372, 1591
Bsp19I CCATGG 1 cut(s) 1368
BspACI CCGC 3 cut(s) 120, 1301, 1588
BspANI GGCC 5 cut(s) 160, 396, 744, 1083, 1429
BspCNI CTCAG 3 cut(s) 184, 1180, 1267
BspDI ATCGAT 1 cut(s) 1446
BspHI TCATGA 2 cut(s) 49, 763
BspLI GGNNCC 2 cut(s) 186, 1593
BspPI GGATC 4 cut(s) 949, 1380, 1586, 1599
BspQI GCTCTTC 1 cut(s) 573
BspTNI GGTCTC 1 cut(s) 1288
BsrBI CCGCTC 1 cut(s) 120
BsrDI GCAATG 3 cut(s) 946, 1125, 1572
BsrI ACTGG 1 cut(s) 667
BssECI CCNNGG 4 cut(s) 651, 1368, 1483, 1555
BssMI GATC 6 cut(s) 52, 941, 1252, 1323, 1372, 1591
BssNAI GTATAC 1 cut(s) 1150
BssT1I CCWWGG 2 cut(s) 1368, 1555
Bst1107I GTATAC 1 cut(s) 1150
Bst2UI CCWGG 3 cut(s) 549, 652, 1695
Bst4CI ACNGT 3 cut(s) 104, 377, 686
Bst6I CTCTTC 3 cut(s) 55, 259, 573
BstBAI YACGTR 3 cut(s) 287, 803, 1640
BstDEI CTNAG 5 cut(s) 192, 487, 993, 1188, 1275
BstDSI CCRYGG 2 cut(s) 1368, 1483
BstF5I GGATG 4 cut(s) 322, 671, 835, 1690
BstKTI GATC 6 cut(s) 55, 944, 1255, 1326, 1375, 1594
BstMAI GTCTC 3 cut(s) 284, 1288, 1517
BstMBI GATC 6 cut(s) 52, 941, 1252, 1323, 1372, 1591
BstMWI GCNNNNNNNGC 2 cut(s) 741, 803
BstNI CCWGG 3 cut(s) 549, 652, 1695
BstNSI RCATGY 2 cut(s) 1177, 1201
BstSCI CCNGG 3 cut(s) 547, 650, 1693
BstSLI GKGCMC 2 cut(s) 808, 1356
BstV1I GCAGC 2 cut(s) 487, 1556
BstV2I GAAGAC 1 cut(s) 1022
BstX2I RGATCY 2 cut(s) 941, 1591
BstXI CCANNNNNNTGG 1 cut(s) 1490
BstYI RGATCY 2 cut(s) 941, 1591
BstZ17I GTATAC 1 cut(s) 1150
Bsu15I ATCGAT 1 cut(s) 1446
BsuRI GGCC 5 cut(s) 160, 396, 744, 1083, 1429
BsuTUI ATCGAT 1 cut(s) 1446
BtgI CCRYGG 2 cut(s) 1368, 1483
BtgZI GCGATG 1 cut(s) 1554
BtsCI GGATG 4 cut(s) 322, 671, 835, 1690
BtsI GCAGTG 1 cut(s) 939
BtsIMutI CAGTG 1 cut(s) 939
CciI TCATGA 2 cut(s) 49, 763
Cfr13I GGNCC 3 cut(s) 158, 545, 743
ClaI ATCGAT 1 cut(s) 1446
Csp6I GTAC 3 cut(s) 759, 984, 1633
CviQI GTAC 3 cut(s) 759, 984, 1633
DdeI CTNAG 5 cut(s) 192, 487, 993, 1188, 1275
DpnI GATC 6 cut(s) 54, 943, 1254, 1325, 1374, 1593
DpnII GATC 6 cut(s) 52, 941, 1252, 1323, 1372, 1591
DriI GACNNNNNGTC 1 cut(s) 343
EaeI YGGCCR 1 cut(s) 1081
Eam1104I CTCTTC 3 cut(s) 55, 259, 573
Eam1105I GACNNNNNGTC 1 cut(s) 343
EarI CTCTTC 3 cut(s) 55, 259, 573
Eco130I CCWWGG 2 cut(s) 1368, 1555
Eco147I AGGCCT 2 cut(s) 396, 1429
Eco31I GGTCTC 1 cut(s) 1288
Eco32I GATATC 1 cut(s) 438
Eco47I GGWCC 1 cut(s) 545
Eco57I CTGAAG 1 cut(s) 1665
EcoO109I RGGNCCY 1 cut(s) 743
EcoRII CCWGG 3 cut(s) 547, 650, 1693
EcoRV GATATC 1 cut(s) 438
EcoT14I CCWWGG 2 cut(s) 1368, 1555
EcoT22I ATGCAT 1 cut(s) 461
ErhI CCWWGG 2 cut(s) 1368, 1555
FaqI GGGAC 3 cut(s) 363, 1402, 1472
FauI CCCGC 1 cut(s) 1581
FbaI TGATCA 1 cut(s) 52
FblI GTMKAC 1 cut(s) 1149
Fnu4HI GCNGC 3 cut(s) 501, 1301, 1570
FokI GGATG 4 cut(s) 329, 678, 842, 1677
Fsp4HI GCNGC 3 cut(s) 501, 1301, 1570
FspBI CTAG 7 cut(s) 306, 513, 570, 893, 965, 1269, 1674
GluI GCNGC 3 cut(s) 501, 1301, 1570
GsaI CCCAGC 1 cut(s) 883
HaeIII GGCC 5 cut(s) 160, 396, 744, 1083, 1429
HapII CCGG 1 cut(s) 161
HincII GTYRAC 4 cut(s) 247, 373, 718, 730
HindII GTYRAC 4 cut(s) 247, 373, 718, 730
HinfI GANTC 6 cut(s) 36, 338, 468, 565, 647, 866
HpaI GTTAAC 2 cut(s) 247, 718
HpaII CCGG 1 cut(s) 161
HphI GGTGA 3 cut(s) 118, 127, 208
Hpy166II GTNNAC 7 cut(s) 247, 373, 718, 730, 806, 1150, 1552
Hpy188I TCNGA 3 cut(s) 349, 855, 1189
Hpy188III TCNNGA 8 cut(s) 33, 50, 314, 602, 764, 1215, 1285, 1604
Hpy8I GTNNAC 7 cut(s) 247, 373, 718, 730, 806, 1150, 1552
HpyAV CCTTC 6 cut(s) 193, 407, 607, 1375, 1605, 1640
HpyCH4III ACNGT 3 cut(s) 104, 377, 686
HpyCH4IV ACGT 3 cut(s) 286, 802, 1639
HpyF10VI GCNNNNNNNGC 2 cut(s) 741, 803
HpyF3I CTNAG 5 cut(s) 192, 487, 993, 1188, 1275
HpySE526I ACGT 3 cut(s) 286, 802, 1639
Ksp22I TGATCA 1 cut(s) 52
KspAI GTTAAC 2 cut(s) 247, 718
Kzo9I GATC 6 cut(s) 52, 941, 1252, 1323, 1372, 1591
LguI GCTCTTC 1 cut(s) 573
LmnI GCTCC 2 cut(s) 202, 1115
Lsp1109I GCAGC 2 cut(s) 487, 1556
LweI GCATC 6 cut(s) 446, 603, 609, 681, 784, 951
MaeI CTAG 7 cut(s) 306, 513, 570, 893, 965, 1269, 1674
MaeII ACGT 3 cut(s) 286, 802, 1639
MaeIII GTNAC 2 cut(s) 282, 1085
MalI GATC 6 cut(s) 54, 943, 1254, 1325, 1374, 1593
MbiI CCGCTC 1 cut(s) 120
MboI GATC 6 cut(s) 52, 941, 1252, 1323, 1372, 1591
MflI RGATCY 2 cut(s) 941, 1591
MhlI GDGCHC 3 cut(s) 808, 892, 1356
MluCI AATT 9 cut(s) 491, 505, 532, 556, 1028, 1319, 1389, 1400, 1599
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 2 cut(s) 660, 668
Mph1103I ATGCAT 1 cut(s) 461
MroXI GAANNNNTTC 1 cut(s) 430
MslI CAYNNNNRTG 4 cut(s) 54, 1041, 1077, 1189
MspA1I CMGCKG 1 cut(s) 1303
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 3 cut(s) 549, 652, 1695
MvaI CCWGG 3 cut(s) 549, 652, 1695
MwoI GCNNNNNNNGC 2 cut(s) 741, 803
NcoI CCATGG 1 cut(s) 1368
NdeII GATC 6 cut(s) 52, 941, 1252, 1323, 1372, 1591
NlaIV GGNNCC 2 cut(s) 186, 1593
NmuCI GTSAC 1 cut(s) 1085
NsiI ATGCAT 1 cut(s) 461
NspI RCATGY 2 cut(s) 1177, 1201
PagI TCATGA 2 cut(s) 49, 763
PceI AGGCCT 2 cut(s) 396, 1429
PciI ACATGT 2 cut(s) 1173, 1197
PciSI GCTCTTC 1 cut(s) 573
PdmI GAANNNNTTC 1 cut(s) 430
PfeI GAWTC 5 cut(s) 36, 468, 565, 647, 866
PkrI GCNGC 3 cut(s) 502, 1302, 1571
PleI GAGTC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 332
Ppu21I YACGTR 3 cut(s) 287, 803, 1640
PscI ACATGT 2 cut(s) 1173, 1197
PshBI ATTAAT 1 cut(s) 1403
Psp6I CCWGG 3 cut(s) 547, 650, 1693
PspFI CCCAGC 1 cut(s) 879
PspGI CCWGG 3 cut(s) 547, 650, 1693
PspN4I GGNNCC 2 cut(s) 186, 1593
PspPI GGNCC 3 cut(s) 158, 545, 743
PsuI RGATCY 2 cut(s) 941, 1591
RsaI GTAC 3 cut(s) 760, 985, 1634
RsaNI GTAC 3 cut(s) 759, 984, 1633
RseI CAYNNNNRTG 4 cut(s) 54, 1041, 1077, 1189
SapI GCTCTTC 1 cut(s) 573
SatI GCNGC 3 cut(s) 501, 1301, 1570
Sau3AI GATC 6 cut(s) 52, 941, 1252, 1323, 1372, 1591
Sau96I GGNCC 3 cut(s) 158, 545, 743
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 3 cut(s) 549, 652, 1695
SduI GDGCHC 3 cut(s) 808, 892, 1356
SfaNI GCATC 6 cut(s) 446, 603, 609, 681, 784, 951
SinI GGWCC 1 cut(s) 545
SmiMI CAYNNNNRTG 4 cut(s) 54, 1041, 1077, 1189
SmlI CTYRAG 2 cut(s) 738, 1625
SmoI CTYRAG 2 cut(s) 738, 1625
Sse9I AATT 9 cut(s) 491, 505, 532, 556, 1028, 1319, 1389, 1400, 1599
SseBI AGGCCT 2 cut(s) 396, 1429
SsiI CCGC 3 cut(s) 120, 1301, 1588
SspI AATATT 3 cut(s) 46, 1465, 1520
SspMI CTAG 7 cut(s) 306, 513, 570, 893, 965, 1269, 1674
StuI AGGCCT 2 cut(s) 396, 1429
StyD4I CCNGG 3 cut(s) 547, 650, 1693
StyI CCWWGG 2 cut(s) 1368, 1555
TaaI ACNGT 3 cut(s) 104, 377, 686
TaiI ACGT 3 cut(s) 289, 805, 1642
TaqI TCGA 6 cut(s) 34, 434, 792, 1214, 1286, 1446
TasI AATT 9 cut(s) 491, 505, 532, 556, 1028, 1319, 1389, 1400, 1599
TatI WGTACW 2 cut(s) 758, 1632
TauI GCSGC 1 cut(s) 1303
TfiI GAWTC 5 cut(s) 36, 468, 565, 647, 866
TscAI CASTG 1 cut(s) 939
TseFI GTSAC 1 cut(s) 1085
TseI GCWGC 2 cut(s) 500, 1569
Tsp45I GTSAC 1 cut(s) 1085
TspRI CASTG 1 cut(s) 939
VneI GTGCAC 1 cut(s) 804
VpaK11BI GGWCC 1 cut(s) 545
VspI ATTAAT 1 cut(s) 1403
XapI RAATTY 4 cut(s) 491, 505, 532, 1028
XceI RCATGY 2 cut(s) 1177, 1201
XcmI CCANNNNNNNNNTGG 1 cut(s) 458
XmiI GTMKAC 1 cut(s) 1149
XmnI GAANNNNTTC 1 cut(s) 430
XspI CTAG 7 cut(s) 306, 513, 570, 893, 965, 1269, 1674
Zsp2I ATGCAT 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.