Rw2G037940

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0009746
Physical Location & Seq
Reverse (-)
62593621 .. 62595705
2085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G037940.1

Sequence Viewer

Length: 1926 bp
ATGAACACATTGGGCATTTTCACTAACATTTCTCGAATCACTAATATTCATGATCATGGAAGAGTTGTTGATAGTAAAAAGGGTAGATTTCAATGCAACTACTGTGATAAAGAGGTGAGCGGTATCACCCGTTTGAAGTGCCATTTAGGAGGAATAGGGCCGGAAGTAAAACCATGTTTAGAGGTTCCTGCTGAGGTGAAGGAGCTGTGGAGAAACAAGTTGCTTGAAAGTCGTGGCAAGAAAGTTAACCACTCTTCCAGCTCAAATGGTGTTAAGCGTAAGAAGCGTGAGACTTCTCAAACTTCTAGTTATCAGGATGGAAGTGAAGAAGATACAGACTCTATGTCAGAAGAAAATGCAACAGATTATGTCAACAGTCCCAATGTAAATCAAAGAGGAGAAAGTAATGACATGAGAAATGTTTCGATATCAATGAACCCCCAAAGATGCATTGGTAGATTCTTTTATGAAACAGGCTTAGAATTTACTGCTGCAAATTCTCCTAGCTTCAAAAGAATGATAAATTCTGTTGCTGGTCCTGGTGTGAATTACAAGATTCCTAGTTGTGAAGAGCTTAAAGGTTGCATCTTTCAAGATGAAGTAAAGGAGATGCAAGAATATGTGAGAAAGATTAAGGAATCCTGGGCTACTACTGGATGTAGCATCTTGTTGGACAGTTGGATTGATGAAAAGGGTAGAAACCTTGTTAACTTTTTGGTCAACTGCCCTCAAGGCCCTGTTTATCTCTGTACTCATGATGTTTCATTTTCCATTGGTAATGTCGATGCCATACGTGCACTATTGGAGGGGATTATTAAGGATGTTGGGGCTGACAATGTTGTTCAGATTGTGGCAGATTCTACAACAGGCTGGGTTGGTGCTCTAGGCAAGGAGTTCATTTTGAAGATTTTGAAGAAACATACTTGTGGCCGTGACCTGATTAAGCCCTCCAAGATAAGAGCAGCAATGCCCTTTATGACTTTAGATAGTATTGTATACGAAAAGCAGGAACTAAAAGACATGTTTGCCTCATCTGAGTGGAACATGTCAATCTGGGGTTCGAGAGTGGAGAGCAAGATGGTAGCAGATATAGTGGAAGATCAGTCCTTTTGGACTAGAGCTGAGATGGTCTCGAAAGCAACTATGCCGCTGGTTCGTGCTATTGAATTGATCTTTGAGGCTGACAAGCCACTTGTGGGGCACATATACGAAACCATGGATCAAGTGAAGGAGAGAATTAAAGAAGAATTAATAAACAAAAAGTCCCAATACTGGCCTTTTTGGGAAGTTATCGATGACATTTGGGACAATATTCTCCATATCCCTCTCCACGGGGCTGGTTATTATCTTAATCCAAGTCTCAAATATTCTAATGACTTCTATTGCGATGATGAGGTTTACCTTGGGCTTATGTGCTGCATTGCACAGATGGTGCGGGATCCTTCAATTCAAGATGTAATACACAATCAACTCAAGGAGTACATACGTGCTGAAGGCAGTTTCAAGGAGGGGAGTTCCTCTAGCCGAAGAATAACTCATCCAGCTATGTGGTGGTCTTCTTATGGGAAACAATGTCCTGAACTCCAGAGGTTTGCCATTAGAGTTTTGAGCCAGAACTGCGATGGTGCTACAAGATATGGACTCAGAAGGACTCTAGCTGAGAAGCTGGTTACAAATGGAAGGAATCCTATCGAGCAGCAACGGTTGAGTGATATGACTTTTCTTCATTATAATTTGCAATTACAGAAGTTTTTCTCAGGTGTGAAGAGCGGCATTGAAGCTGAGGATATTCATCCAATGGATGACTGGATCATCGATGAAGCACAAGATATTGTTTCTCAGAATGGGGATGTGAAGGTGGAGCCTGCTAATGGAGCTACCAATGAAGGATGTTCAAGATTTCAGCTTAAAATAGAATCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

641

Amino Acids

72.77

Weight (kDa)

5.84

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 187 - 298 3e-35 Domain of unknown function (DUF659)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009746)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13010 AT3G13020 AT3G13030 AT3G13030 AT3G13030 AT3G13030 AT3G13030
fragaria_vesca FvH4_6g36422
malus_domestica MD09G1169400.v1.1
pyrus_communis pycom09g08680
rosa_chinensis RchiOBHm_Chr2g0148401
rosa_laevigata RLG00000020348
rosa_roxburghii Rroxscaffold_2G00098760
rosa_rugosa Rorug02G0406800
rosa_samantha Rh2AG465500 Rh2BG478400 Rh2BG478500 Rh2CG452000 Rh2DG487200 Rh2DG487300
rosa_wichuraiana Rw2G037940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1731
AccBSI CCGCTC 2 cut(s) 120, 1770
AccI GTMKAC 1 cut(s) 996
AciI CCGC 4 cut(s) 120, 1148, 1435, 1770
AclWI GGATC 4 cut(s) 1227, 1433, 1446, 1817
AcoI YGGCCR 1 cut(s) 928
AcsI RAATTY 3 cut(s) 482, 496, 523
AcuI CTGAAG 1 cut(s) 1512
AfaI GTAC 2 cut(s) 751, 1481
AfiI CCNNNNNNNGG 4 cut(s) 1196, 1272, 1331, 1871
AflIII ACRYGT 2 cut(s) 1020, 1044
AhdI GACNNNNNGTC 1 cut(s) 343
AjnI CCWGG 2 cut(s) 538, 641
Alw21I GWGCWC 2 cut(s) 799, 883
Alw26I GTCTC 3 cut(s) 284, 1135, 1364
Alw44I GTGCAC 1 cut(s) 795
AlwI GGATC 4 cut(s) 1227, 1433, 1446, 1817
AoxI GGCC 4 cut(s) 158, 733, 928, 1274
ApaLI GTGCAC 1 cut(s) 795
ApeKI GCWGC 4 cut(s) 491, 962, 1416, 1696
ApoI RAATTY 3 cut(s) 482, 496, 523
AseI ATTAAT 1 cut(s) 1250
Asp700I GAANNNNTTC 2 cut(s) 421, 1751
AspS9I GGNCC 3 cut(s) 158, 536, 734
AsuHPI GGTGA 3 cut(s) 118, 127, 208
AvaII GGWCC 1 cut(s) 536
BaeGI GKGCMC 2 cut(s) 799, 1203
BamHI GGATCC 1 cut(s) 1438
BbsI GAAGAC 1 cut(s) 1548
Bbv12I GWGCWC 2 cut(s) 799, 883
BbvCI CCTCAGC 2 cut(s) 192, 1782
BbvI GCAGC 4 cut(s) 478, 974, 1403, 1708
BccI CCATC 5 cut(s) 311, 1072, 1120, 1423, 1616
BceAI ACGGC 1 cut(s) 915
BciT130I CCWGG 2 cut(s) 540, 643
BclI TGATCA 1 cut(s) 52
BcoDI GTCTC 3 cut(s) 284, 1135, 1364
BfaI CTAG 7 cut(s) 306, 504, 561, 884, 1116, 1521, 1655
BglI GCCNNNNNGGC 1 cut(s) 732
BisI GCNGC 6 cut(s) 492, 963, 1148, 1417, 1697, 1771
BlsI GCNGC 6 cut(s) 493, 964, 1149, 1418, 1698, 1772
Bme1390I CCNGG 2 cut(s) 540, 643
Bme18I GGWCC 1 cut(s) 536
BmeRI GACNNNNNGTC 1 cut(s) 343
BmgT120I GGNCC 3 cut(s) 158, 536, 734
BmiI GGNNCC 3 cut(s) 186, 1440, 1863
BmrFI CCNGG 2 cut(s) 540, 643
BmsI GCATC 5 cut(s) 437, 594, 600, 672, 775
BpiI GAAGAC 1 cut(s) 1548
BplI GAGNNNNNCTC 2 cut(s) 1115, 1147
BpmI CTGGAG 1 cut(s) 1568
Bpu10I CCTNAGC 2 cut(s) 192, 1782
BpuEI CTTGAG 2 cut(s) 714, 1457
Bsa29I ATCGAT 2 cut(s) 1293, 1815
BsaAI YACGTR 2 cut(s) 794, 1487
BsaBI GATNNNNATC 1 cut(s) 1791
BsaI GGTCTC 1 cut(s) 1135
BsaJI CCNNGG 4 cut(s) 642, 1215, 1330, 1402
Bsc4I CCNNNNNNNGG 4 cut(s) 1196, 1272, 1331, 1871
Bse1I ACTGG 3 cut(s) 658, 1277, 1811
Bse3DI GCAATG 2 cut(s) 972, 1419
Bse8I GATNNNNATC 1 cut(s) 1791
BseBI CCWGG 2 cut(s) 540, 643
BseCI ATCGAT 2 cut(s) 1293, 1815
BseDI CCNNGG 4 cut(s) 642, 1215, 1330, 1402
BseGI GGATG 8 cut(s) 322, 662, 826, 1537, 1792, 1807, 1855, 1895
BseJI GATNNNNATC 1 cut(s) 1791
BseLI CCNNNNNNNGG 4 cut(s) 1196, 1272, 1331, 1871
BseMI GCAATG 2 cut(s) 972, 1419
BseMII CTCAG 8 cut(s) 183, 1026, 1113, 1650, 1657, 1770, 1773, 1853
BseNI ACTGG 3 cut(s) 658, 1277, 1811
BseRI GAGGAG 1 cut(s) 411
BseSI GKGCMC 2 cut(s) 799, 1203
BseXI GCAGC 4 cut(s) 478, 974, 1403, 1708
BseYI CCCAGC 1 cut(s) 870
BshFI GGCC 4 cut(s) 160, 735, 930, 1276
BshVI ATCGAT 2 cut(s) 1293, 1815
BsiHKAI GWGCWC 2 cut(s) 799, 883
BsiSI CCGG 1 cut(s) 161
BslFI GGGAC 3 cut(s) 363, 1249, 1319
BslI CCNNNNNNNGG 4 cut(s) 1196, 1272, 1331, 1871
BsmAI GTCTC 3 cut(s) 284, 1135, 1364
BsmFI GGGAC 3 cut(s) 363, 1249, 1319
BsnI GGCC 4 cut(s) 160, 735, 930, 1276
Bso31I GGTCTC 1 cut(s) 1135
Bsp1286I GDGCHC 3 cut(s) 799, 883, 1203
Bsp143I GATC 6 cut(s) 52, 1099, 1170, 1219, 1438, 1809
Bsp19I CCATGG 1 cut(s) 1215
BspACI CCGC 4 cut(s) 120, 1148, 1435, 1770
BspANI GGCC 4 cut(s) 160, 735, 930, 1276
BspCNI CTCAG 8 cut(s) 184, 1027, 1114, 1651, 1656, 1769, 1774, 1852
BspDI ATCGAT 2 cut(s) 1293, 1815
BspHI TCATGA 2 cut(s) 49, 754
BspLI GGNNCC 3 cut(s) 186, 1440, 1863
BspPI GGATC 4 cut(s) 1227, 1433, 1446, 1817
BspQI GCTCTTC 2 cut(s) 564, 1760
BspTNI GGTCTC 1 cut(s) 1135
BsrBI CCGCTC 2 cut(s) 120, 1770
BsrDI GCAATG 2 cut(s) 972, 1419
BsrI ACTGG 3 cut(s) 658, 1277, 1811
BssECI CCNNGG 4 cut(s) 642, 1215, 1330, 1402
BssMI GATC 6 cut(s) 52, 1099, 1170, 1219, 1438, 1809
BssNAI GTATAC 1 cut(s) 997
BssT1I CCWWGG 2 cut(s) 1215, 1402
Bst1107I GTATAC 1 cut(s) 997
Bst2UI CCWGG 2 cut(s) 540, 643
Bst4CI ACNGT 4 cut(s) 104, 377, 677, 1704
Bst6I CTCTTC 4 cut(s) 55, 259, 564, 1760
BstBAI YACGTR 2 cut(s) 794, 1487
BstC8I GCNNGC 1 cut(s) 1866
BstDEI CTNAG 9 cut(s) 192, 478, 1035, 1122, 1643, 1659, 1756, 1782, 1839
BstDSI CCRYGG 2 cut(s) 1215, 1330
BstF5I GGATG 8 cut(s) 322, 662, 826, 1537, 1792, 1807, 1855, 1895
BstKTI GATC 6 cut(s) 55, 1102, 1173, 1222, 1441, 1812
BstMAI GTCTC 3 cut(s) 284, 1135, 1364
BstMBI GATC 6 cut(s) 52, 1099, 1170, 1219, 1438, 1809
BstMWI GCNNNNNNNGC 5 cut(s) 283, 732, 794, 1617, 1874
BstNI CCWGG 2 cut(s) 540, 643
BstNSI RCATGY 2 cut(s) 1024, 1048
BstSCI CCNGG 2 cut(s) 538, 641
BstSLI GKGCMC 2 cut(s) 799, 1203
BstV1I GCAGC 4 cut(s) 478, 974, 1403, 1708
BstV2I GAAGAC 1 cut(s) 1548
BstX2I RGATCY 1 cut(s) 1438
BstXI CCANNNNNNTGG 2 cut(s) 1337, 1548
BstYI RGATCY 1 cut(s) 1438
BstZ17I GTATAC 1 cut(s) 997
Bsu15I ATCGAT 2 cut(s) 1293, 1815
BsuRI GGCC 4 cut(s) 160, 735, 930, 1276
BsuTUI ATCGAT 2 cut(s) 1293, 1815
BtgI CCRYGG 2 cut(s) 1215, 1330
BtgZI GCGATG 2 cut(s) 1401, 1635
BtsCI GGATG 8 cut(s) 322, 662, 826, 1537, 1792, 1807, 1855, 1895
Cac8I GCNNGC 1 cut(s) 1866
CciI TCATGA 2 cut(s) 49, 754
Cfr13I GGNCC 3 cut(s) 158, 536, 734
ClaI ATCGAT 2 cut(s) 1293, 1815
Csp6I GTAC 2 cut(s) 750, 1480
CviAII CATG 9 cut(s) 50, 56, 174, 412, 755, 1021, 1045, 1216, 1921
CviQI GTAC 2 cut(s) 750, 1480
DdeI CTNAG 9 cut(s) 192, 478, 1035, 1122, 1643, 1659, 1756, 1782, 1839
DpnI GATC 6 cut(s) 54, 1101, 1172, 1221, 1440, 1811
DpnII GATC 6 cut(s) 52, 1099, 1170, 1219, 1438, 1809
DriI GACNNNNNGTC 1 cut(s) 343
EaeI YGGCCR 1 cut(s) 928
Eam1104I CTCTTC 4 cut(s) 55, 259, 564, 1760
Eam1105I GACNNNNNGTC 1 cut(s) 343
EarI CTCTTC 4 cut(s) 55, 259, 564, 1760
Eco130I CCWWGG 2 cut(s) 1215, 1402
Eco31I GGTCTC 1 cut(s) 1135
Eco32I GATATC 1 cut(s) 429
Eco47I GGWCC 1 cut(s) 536
Eco57I CTGAAG 1 cut(s) 1512
EcoO109I RGGNCCY 1 cut(s) 734
EcoRII CCWGG 2 cut(s) 538, 641
EcoRV GATATC 1 cut(s) 429
EcoT14I CCWWGG 2 cut(s) 1215, 1402
EcoT22I ATGCAT 1 cut(s) 452
ErhI CCWWGG 2 cut(s) 1215, 1402
FaeI CATG 9 cut(s) 53, 59, 177, 415, 758, 1024, 1048, 1219, 1924
FaqI GGGAC 3 cut(s) 363, 1249, 1319
FatI CATG 9 cut(s) 49, 55, 173, 411, 754, 1020, 1044, 1215, 1920
FauI CCCGC 1 cut(s) 1428
FbaI TGATCA 1 cut(s) 52
FblI GTMKAC 1 cut(s) 996
Fnu4HI GCNGC 6 cut(s) 492, 963, 1148, 1417, 1697, 1771
FokI GGATG 8 cut(s) 329, 669, 833, 1524, 1779, 1814, 1862, 1902
Fsp4HI GCNGC 6 cut(s) 492, 963, 1148, 1417, 1697, 1771
FspBI CTAG 7 cut(s) 306, 504, 561, 884, 1116, 1521, 1655
GluI GCNGC 6 cut(s) 492, 963, 1148, 1417, 1697, 1771
GsaI CCCAGC 1 cut(s) 874
GsuI CTGGAG 1 cut(s) 1568
HaeIII GGCC 4 cut(s) 160, 735, 930, 1276
HapII CCGG 1 cut(s) 161
Hin1II CATG 9 cut(s) 53, 59, 177, 415, 758, 1024, 1048, 1219, 1924
HincII GTYRAC 4 cut(s) 247, 373, 709, 721
HindII GTYRAC 4 cut(s) 247, 373, 709, 721
HpaI GTTAAC 2 cut(s) 247, 709
HpaII CCGG 1 cut(s) 161
HphI GGTGA 3 cut(s) 118, 127, 208
Hpy166II GTNNAC 7 cut(s) 247, 373, 709, 721, 797, 997, 1399
Hpy188I TCNGA 5 cut(s) 349, 846, 1036, 1646, 1842
Hpy8I GTNNAC 7 cut(s) 247, 373, 709, 721, 797, 997, 1399
HpyAV CCTTC 8 cut(s) 193, 1222, 1452, 1487, 1641, 1674, 1849, 1880
HpyCH4III ACNGT 4 cut(s) 104, 377, 677, 1704
HpyCH4IV ACGT 2 cut(s) 793, 1486
HpyF10VI GCNNNNNNNGC 5 cut(s) 283, 732, 794, 1617, 1874
HpyF3I CTNAG 9 cut(s) 192, 478, 1035, 1122, 1643, 1659, 1756, 1782, 1839
HpySE526I ACGT 2 cut(s) 793, 1486
Hsp92II CATG 9 cut(s) 53, 59, 177, 415, 758, 1024, 1048, 1219, 1924
Ksp22I TGATCA 1 cut(s) 52
KspAI GTTAAC 2 cut(s) 247, 709
Kzo9I GATC 6 cut(s) 52, 1099, 1170, 1219, 1438, 1809
LguI GCTCTTC 2 cut(s) 564, 1760
LmnI GCTCC 3 cut(s) 202, 1861, 1874
Lsp1109I GCAGC 4 cut(s) 478, 974, 1403, 1708
LweI GCATC 5 cut(s) 437, 594, 600, 672, 775
MaeI CTAG 7 cut(s) 306, 504, 561, 884, 1116, 1521, 1655
MaeII ACGT 2 cut(s) 793, 1486
MaeIII GTNAC 2 cut(s) 932, 1669
MalI GATC 6 cut(s) 54, 1101, 1172, 1221, 1440, 1811
MbiI CCGCTC 2 cut(s) 120, 1770
MboI GATC 6 cut(s) 52, 1099, 1170, 1219, 1438, 1809
MflI RGATCY 1 cut(s) 1438
MhlI GDGCHC 3 cut(s) 799, 883, 1203
MlyI GAGTC 3 cut(s) 332, 1635, 1645
MmeI TCCRAC 2 cut(s) 651, 659
Mph1103I ATGCAT 1 cut(s) 452
MroXI GAANNNNTTC 2 cut(s) 421, 1751
MslI CAYNNNNRTG 3 cut(s) 54, 924, 1036
MspA1I CMGCKG 1 cut(s) 1150
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 2 cut(s) 540, 643
MvaI CCWGG 2 cut(s) 540, 643
MwoI GCNNNNNNNGC 5 cut(s) 283, 732, 794, 1617, 1874
NcoI CCATGG 1 cut(s) 1215
NdeII GATC 6 cut(s) 52, 1099, 1170, 1219, 1438, 1809
NlaIII CATG 9 cut(s) 53, 59, 177, 415, 758, 1024, 1048, 1219, 1924
NlaIV GGNNCC 3 cut(s) 186, 1440, 1863
NmuCI GTSAC 1 cut(s) 932
NsiI ATGCAT 1 cut(s) 452
NspI RCATGY 2 cut(s) 1024, 1048
PagI TCATGA 2 cut(s) 49, 754
PciI ACATGT 2 cut(s) 1020, 1044
PciSI GCTCTTC 2 cut(s) 564, 1760
PdmI GAANNNNTTC 2 cut(s) 421, 1751
PfeI GAWTC 7 cut(s) 36, 459, 556, 638, 857, 1684, 1916
PkrI GCNGC 6 cut(s) 493, 964, 1149, 1418, 1698, 1772
PleI GAGTC 3 cut(s) 332, 1635, 1645
PpsI GAGTC 3 cut(s) 332, 1635, 1645
Ppu21I YACGTR 2 cut(s) 794, 1487
PscI ACATGT 2 cut(s) 1020, 1044
PshBI ATTAAT 1 cut(s) 1250
PsiI TTATAA 1 cut(s) 1731
Psp6I CCWGG 2 cut(s) 538, 641
PspFI CCCAGC 1 cut(s) 870
PspGI CCWGG 2 cut(s) 538, 641
PspN4I GGNNCC 3 cut(s) 186, 1440, 1863
PspPI GGNCC 3 cut(s) 158, 536, 734
PsuI RGATCY 1 cut(s) 1438
RsaI GTAC 2 cut(s) 751, 1481
RsaNI GTAC 2 cut(s) 750, 1480
RseI CAYNNNNRTG 3 cut(s) 54, 924, 1036
SapI GCTCTTC 2 cut(s) 564, 1760
SatI GCNGC 6 cut(s) 492, 963, 1148, 1417, 1697, 1771
Sau3AI GATC 6 cut(s) 52, 1099, 1170, 1219, 1438, 1809
Sau96I GGNCC 3 cut(s) 158, 536, 734
SchI GAGTC 3 cut(s) 332, 1635, 1645
ScrFI CCNGG 2 cut(s) 540, 643
SduI GDGCHC 3 cut(s) 799, 883, 1203
SfaNI GCATC 5 cut(s) 437, 594, 600, 672, 775
SinI GGWCC 1 cut(s) 536
SmiMI CAYNNNNRTG 3 cut(s) 54, 924, 1036
SmlI CTYRAG 2 cut(s) 729, 1472
SmoI CTYRAG 2 cut(s) 729, 1472
SsiI CCGC 4 cut(s) 120, 1148, 1435, 1770
SspI AATATT 3 cut(s) 46, 1312, 1367
SspMI CTAG 7 cut(s) 306, 504, 561, 884, 1116, 1521, 1655
StyD4I CCNGG 2 cut(s) 538, 641
StyI CCWWGG 2 cut(s) 1215, 1402
TaaI ACNGT 4 cut(s) 104, 377, 677, 1704
TaiI ACGT 2 cut(s) 796, 1489
TaqI TCGA 8 cut(s) 34, 425, 783, 1061, 1133, 1293, 1692, 1815
TatI WGTACW 2 cut(s) 749, 1479
TauI GCSGC 2 cut(s) 1150, 1773
TfiI GAWTC 7 cut(s) 36, 459, 556, 638, 857, 1684, 1916
TseFI GTSAC 1 cut(s) 932
TseI GCWGC 4 cut(s) 491, 962, 1416, 1696
Tsp45I GTSAC 1 cut(s) 932
VneI GTGCAC 1 cut(s) 795
VpaK11BI GGWCC 1 cut(s) 536
VspI ATTAAT 1 cut(s) 1250
XapI RAATTY 3 cut(s) 482, 496, 523
XceI RCATGY 2 cut(s) 1024, 1048
XcmI CCANNNNNNNNNTGG 4 cut(s) 449, 1548, 1619, 1803
XmiI GTMKAC 1 cut(s) 996
XmnI GAANNNNTTC 2 cut(s) 421, 1751
XspI CTAG 7 cut(s) 306, 504, 561, 884, 1116, 1521, 1655
Zsp2I ATGCAT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.